Rw6G017470

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000053
Physical Location & Seq
Reverse (-)
34641222 .. 34644883
3662 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G017470.1

Sequence Viewer

Length: 1539 bp
ATGACCAAAGTGAAGTTTTTAAAAAAAGAAATTGGAGAACTTAAAGAAGTGGTGAGAATGGATTTCTTAGCAGCGGCTACAGGTCATGATATTTCTGCAAAACTAAAGTTGATCGAAGCAGTTGAGCGCCTAGGTCTGGCTTACCACTTTGAAACAGAGATAGAAGAAGCATTGGAGGGCATATATGCTACTACCGGCTATAATTCTCATGAGGATGGTGATCTTCACAATGTCACCCTGCGATTTCGGTTGCTTAGACAACATGGATACAATGTCTCAACTGAAATGTTTAACAAGTTCAGAGATGACAAAGGCAACTTCAAGGAAAGCTTGATTACTGACATACAGGGCTTGATAAGTTTGTACGAAGCTGCACATCTTGCTGTCCATGGGGAAGATATATTAGATGAAGCTTTGGCTTTCACTACTGCTCATCTTAATGCAATGGTAAACAACCCTAACTTGGCAGCAGAAGTAACTCATGTTTTGAAGCAACTACCATATAAGGGCATACCAAGGCTAGAGGCCAAGAAATACATCTCCATCTACCAGGATATGGCCTCCCATAACCCAGCTCTCTTAAAGCTTGCAAAGTTGGATTTCAATCTAGTGCAGTCATTGCACAAAAAGGAGCTTAGTATTGTTGCCAGGTGGTGGAAAGATTTAGGGTTTGCCACAAAGCTTACATTTGCAAGAGACAGACTTGTGGAGTGCTATTTTTGGTCTGTGGCAACTTATTTCGAACCCCAATACTTGCTTGCCAGAAGAATTTTAACAAAAGTAATTGCCATCACTGTTATCATCGATGATATCTATGATGCATACGGCACCATTGATGAACTCACTCTCTTCACTGAAGCAATTCAAAGGTGGGATATCAACTGCATGAATCAGCTTCCAGAAAAACTGCAACTCTGTTACAAGGCACTTATCGAGTTTTTTGAAGAAATTGAAGATGAAATGGCAAAGGAAGGAAGATCATACCGAGTTCATTATGCAAAAGAGACGATGAAAGCTCTATGCCGAGGTTACTTAAAAGAAGCTCAATGTTTCCATGAAGACTACATCCCAAGTGTGGAGGAGCATATGGAACTTGCACTTGTAACCTGCACCTATCCAATGCTTCTAACTCTAGCTTTAGTTGGCATGGGCAGCAATGTAACAAGGGAGACCTTTGAGTGGATGTCCCAGAGGCCTAAGATTCTTACAGCTTCAGCAACCATTTCGAGGTGCATGGACGACATTGTTGGACACAAGAGGGGGCACGCAGCGTCAGGAGTTGAATGCTACATGAAACAATATGGTGTGTCGGAAAAAGAAGCATGTGATGTGCTTCAAAAGAGAGTCGATAGTGCCTGGAAGGATATAAACTCAGAGATGCTGAAACCAACAGAAATGCCAATTCCAATTCTTCTACCGATTCTGAACTTTGCACGAGTCATGGATGTCCTGTACAAGGTGGAAGATAGCTTCACGCATGTCGCACAACCAGTAAAAGATGGCATTTCTTCATTACTGATAGACCCCGTTCAGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

512

Amino Acids

58.56

Weight (kDa)

5.64

Isoelectric Point (pI)

40.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 6 - 163 1e-53 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 220 - 455 3.6e-98 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 260 - 455 3.3e-57 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1115
AccB1I GGYRCC 1 cut(s) 827
AccB7I CCANNNNNTGG 2 cut(s) 556, 654
AciI CCGC 1 cut(s) 74
AcsI RAATTY 1 cut(s) 768
AcuI CTGAAG 2 cut(s) 876, 1197
AfaI GTAC 2 cut(s) 365, 1454
AfiI CCNNNNNNNGG 9 cut(s) 136, 463, 506, 556, 654, 1075, 1179, 1227, 1456
AgsI TTSAA 9 cut(s) 152, 322, 490, 604, 866, 944, 953, 1283, 1337
AjnI CCWGG 3 cut(s) 549, 647, 1355
Alw26I GTCTC 4 cut(s) 280, 690, 998, 1163
AoxI GGCC 3 cut(s) 525, 558, 1193
ApeKI GCWGC 5 cut(s) 71, 371, 467, 1152, 1268
ApoI RAATTY 1 cut(s) 768
Asp700I GAANNNNTTC 1 cut(s) 861
AspA2I CCTAGG 1 cut(s) 130
AspLEI GCGC 1 cut(s) 129
AsuHPI GGTGA 3 cut(s) 64, 226, 230
AsuII TTCGAA 1 cut(s) 741
AvrII CCTAGG 1 cut(s) 130
BaeGI GKGCMC 1 cut(s) 1266
BanI GGYRCC 1 cut(s) 827
BauI CACGAG 1 cut(s) 1434
BbsI GAAGAC 1 cut(s) 1065
BbvI GCAGC 5 cut(s) 83, 358, 479, 1164, 1280
BccI CCATC 4 cut(s) 209, 551, 797, 1493
BceAI ACGGC 1 cut(s) 841
BcgI CGANNNNNNTGC 2 cut(s) 1206, 1240
BciT130I CCWGG 3 cut(s) 551, 649, 1357
BciVI GTATCC 1 cut(s) 260
BcoDI GTCTC 4 cut(s) 280, 690, 998, 1163
BfaI CTAG 4 cut(s) 131, 521, 608, 1133
BfmI CTRYAG 1 cut(s) 78
BfoI RGCGCY 1 cut(s) 130
BfuAI ACCTGC 1 cut(s) 1115
BfuI GTATCC 1 cut(s) 260
BisI GCNGC 6 cut(s) 72, 75, 372, 468, 1153, 1269
BlnI CCTAGG 1 cut(s) 130
BlsI GCNGC 6 cut(s) 73, 76, 373, 469, 1154, 1270
Bme1390I CCNGG 3 cut(s) 551, 649, 1357
BmiI GGNNCC 1 cut(s) 829
BmrFI CCNGG 3 cut(s) 551, 649, 1357
BmsI GCATC 2 cut(s) 808, 1368
BpiI GAAGAC 1 cut(s) 1065
Bpu14I TTCGAA 1 cut(s) 741
Bsa29I ATCGAT 1 cut(s) 804
BsaBI GATNNNNATC 2 cut(s) 219, 603
BsaI GGTCTC 1 cut(s) 1163
BsaJI CCNNGG 4 cut(s) 130, 388, 515, 1024
Bsc4I CCNNNNNNNGG 9 cut(s) 136, 463, 506, 556, 654, 1075, 1179, 1227, 1456
Bse118I RCCGGY 1 cut(s) 194
Bse1I ACTGG 1 cut(s) 1490
Bse3DI GCAATG 3 cut(s) 450, 617, 1162
Bse8I GATNNNNATC 2 cut(s) 219, 603
BseBI CCWGG 3 cut(s) 551, 649, 1357
BseCI ATCGAT 1 cut(s) 804
BseDI CCNNGG 4 cut(s) 130, 388, 515, 1024
BseGI GGATG 4 cut(s) 220, 1065, 1188, 1450
BseJI GATNNNNATC 2 cut(s) 219, 603
BseLI CCNNNNNNNGG 9 cut(s) 136, 463, 506, 556, 654, 1075, 1179, 1227, 1456
BseMI GCAATG 3 cut(s) 450, 617, 1162
BseMII CTCAG 1 cut(s) 1386
BseNI ACTGG 1 cut(s) 1490
BseRI GAGGAG 1 cut(s) 1094
BseSI GKGCMC 1 cut(s) 1266
BseXI GCAGC 5 cut(s) 83, 358, 479, 1164, 1280
BseYI CCCAGC 1 cut(s) 571
BsgI GTGCAG 3 cut(s) 357, 632, 1093
BshFI GGCC 3 cut(s) 527, 560, 1195
BshNI GGYRCC 1 cut(s) 827
BshVI ATCGAT 1 cut(s) 804
BsiSI CCGG 1 cut(s) 195
BslFI GGGAC 1 cut(s) 1171
BslI CCNNNNNNNGG 9 cut(s) 136, 463, 506, 556, 654, 1075, 1179, 1227, 1456
BsmAI GTCTC 4 cut(s) 280, 690, 998, 1163
BsmBI CGTCTC 1 cut(s) 998
BsmFI GGGAC 1 cut(s) 1171
BsmI GAATGC 1 cut(s) 1289
BsnI GGCC 3 cut(s) 527, 560, 1195
Bso31I GGTCTC 1 cut(s) 1163
Bsp119I TTCGAA 1 cut(s) 741
Bsp1286I GDGCHC 1 cut(s) 1266
Bsp1407I TGTACA 1 cut(s) 1452
Bsp143I GATC 3 cut(s) 111, 220, 977
Bsp19I CCATGG 1 cut(s) 388
BspACI CCGC 1 cut(s) 74
BspANI GGCC 3 cut(s) 527, 560, 1195
BspCNI CTCAG 1 cut(s) 1385
BspDI ATCGAT 1 cut(s) 804
BspHI TCATGA 2 cut(s) 85, 208
BspLI GGNNCC 1 cut(s) 829
BspMI ACCTGC 1 cut(s) 1115
BspT104I TTCGAA 1 cut(s) 741
BspT107I GGYRCC 1 cut(s) 827
BspTNI GGTCTC 1 cut(s) 1163
BsrDI GCAATG 3 cut(s) 450, 617, 1162
BsrFI RCCGGY 1 cut(s) 194
BsrGI TGTACA 1 cut(s) 1452
BsrI ACTGG 1 cut(s) 1490
BssAI RCCGGY 1 cut(s) 194
BssECI CCNNGG 4 cut(s) 130, 388, 515, 1024
BssMI GATC 3 cut(s) 111, 220, 977
BssSI CACGAG 1 cut(s) 1434
BssT1I CCWWGG 3 cut(s) 130, 388, 515
Bst2BI CACGAG 1 cut(s) 1434
Bst2UI CCWGG 3 cut(s) 551, 649, 1357
Bst4CI ACNGT 1 cut(s) 796
Bst6I CTCTTC 1 cut(s) 854
BstAPI GCANNNNNTGC 2 cut(s) 380, 619
BstAUI TGTACA 1 cut(s) 1452
BstBI TTCGAA 1 cut(s) 741
BstC8I GCNNGC 3 cut(s) 588, 759, 1266
BstDEI CTNAG 5 cut(s) 67, 254, 635, 1197, 1372
BstDSI CCRYGG 1 cut(s) 388
BstENI CCTNNNNNAGG 1 cut(s) 1454
BstF5I GGATG 4 cut(s) 220, 1065, 1188, 1450
BstH2I RGCGCY 1 cut(s) 130
BstHHI GCGC 1 cut(s) 129
BstKTI GATC 3 cut(s) 114, 223, 980
BstMAI GTCTC 4 cut(s) 280, 690, 998, 1163
BstMBI GATC 3 cut(s) 111, 220, 977
BstMWI GCNNNNNNNGC 3 cut(s) 380, 619, 1152
BstNI CCWGG 3 cut(s) 551, 649, 1357
BstNSI RCATGY 2 cut(s) 1326, 1481
BstSCI CCNGG 3 cut(s) 549, 647, 1355
BstSFI CTRYAG 1 cut(s) 78
BstSLI GKGCMC 1 cut(s) 1266
BstV1I GCAGC 5 cut(s) 83, 358, 479, 1164, 1280
BstV2I GAAGAC 1 cut(s) 1065
Bsu15I ATCGAT 1 cut(s) 804
BsuI GTATCC 1 cut(s) 260
BsuRI GGCC 3 cut(s) 527, 560, 1195
BsuTUI ATCGAT 1 cut(s) 804
BtgI CCRYGG 1 cut(s) 388
BtsCI GGATG 4 cut(s) 220, 1065, 1188, 1450
BtsIMutI CAGTG 2 cut(s) 792, 852
BveI ACCTGC 1 cut(s) 1115
Cac8I GCNNGC 3 cut(s) 588, 759, 1266
CciI TCATGA 2 cut(s) 85, 208
CfoI GCGC 1 cut(s) 129
Cfr10I RCCGGY 1 cut(s) 194
ClaI ATCGAT 1 cut(s) 804
CseI GACGC 1 cut(s) 1260
Csp6I GTAC 2 cut(s) 364, 1453
CviQI GTAC 2 cut(s) 364, 1453
DdeI CTNAG 5 cut(s) 67, 254, 635, 1197, 1372
DpnI GATC 3 cut(s) 113, 222, 979
DpnII GATC 3 cut(s) 111, 220, 977
DraI TTTAAA 1 cut(s) 21
Eam1104I CTCTTC 1 cut(s) 854
EarI CTCTTC 1 cut(s) 854
Eco130I CCWWGG 3 cut(s) 130, 388, 515
Eco147I AGGCCT 1 cut(s) 1195
Eco31I GGTCTC 1 cut(s) 1163
Eco32I GATATC 2 cut(s) 811, 877
Eco57I CTGAAG 2 cut(s) 876, 1197
EcoNI CCTNNNNNAGG 1 cut(s) 1454
EcoRII CCWGG 3 cut(s) 549, 647, 1355
EcoRV GATATC 2 cut(s) 811, 877
EcoT14I CCWWGG 3 cut(s) 130, 388, 515
EcoT22I ATGCAT 1 cut(s) 823
ErhI CCWWGG 3 cut(s) 130, 388, 515
Esp3I CGTCTC 1 cut(s) 998
FalI AAGNNNNNCTT 2 cut(s) 314, 346
FaqI GGGAC 1 cut(s) 1171
FauNDI CATATG 1 cut(s) 1086
Fnu4HI GCNGC 6 cut(s) 72, 75, 372, 468, 1153, 1269
FokI GGATG 4 cut(s) 227, 1052, 1195, 1457
Fsp4HI GCNGC 6 cut(s) 72, 75, 372, 468, 1153, 1269
FspBI CTAG 4 cut(s) 131, 521, 608, 1133
GlaI GCGC 1 cut(s) 128
GluI GCNGC 6 cut(s) 72, 75, 372, 468, 1153, 1269
GsaI CCCAGC 1 cut(s) 575
HaeII RGCGCY 1 cut(s) 130
HaeIII GGCC 3 cut(s) 527, 560, 1195
HapII CCGG 1 cut(s) 195
HgaI GACGC 1 cut(s) 1260
HhaI GCGC 1 cut(s) 129
Hin6I GCGC 1 cut(s) 127
HinP1I GCGC 1 cut(s) 127
HindIII AAGCTT 4 cut(s) 328, 411, 584, 680
HinfI GANTC 5 cut(s) 889, 1201, 1344, 1420, 1437
HpaII CCGG 1 cut(s) 195
HphI GGTGA 3 cut(s) 64, 226, 230
Hpy166II GTNNAC 1 cut(s) 451
Hpy188I TCNGA 5 cut(s) 302, 1312, 1375, 1425, 1533
Hpy188III TCNNGA 4 cut(s) 86, 209, 899, 1275
Hpy8I GTNNAC 1 cut(s) 451
HpyAV CCTTC 2 cut(s) 965, 1354
HpyCH4III ACNGT 1 cut(s) 796
HpyF10VI GCNNNNNNNGC 3 cut(s) 380, 619, 1152
HpyF3I CTNAG 5 cut(s) 67, 254, 635, 1197, 1372
HspAI GCGC 1 cut(s) 127
Kzo9I GATC 3 cut(s) 111, 220, 977
LmnI GCTCC 2 cut(s) 631, 1081
Lsp1109I GCAGC 5 cut(s) 83, 358, 479, 1164, 1280
LweI GCATC 2 cut(s) 808, 1368
MaeI CTAG 4 cut(s) 131, 521, 608, 1133
MaeIII GTNAC 6 cut(s) 232, 475, 917, 1028, 1102, 1159
MalI GATC 3 cut(s) 113, 222, 979
MboI GATC 3 cut(s) 111, 220, 977
MhlI GDGCHC 1 cut(s) 1266
MluCI AATT 8 cut(s) 30, 202, 768, 783, 861, 948, 1401, 1407
MlyI GAGTC 2 cut(s) 1353, 1446
MmeI TCCRAC 3 cut(s) 576, 1228, 1290
MnlI CCTC 9 cut(s) 169, 205, 517, 571, 1019, 1072, 1185, 1221, 1251
Mph1103I ATGCAT 1 cut(s) 823
MroXI GAANNNNTTC 1 cut(s) 861
MseI TTAA 7 cut(s) 20, 42, 291, 438, 581, 773, 1034
MslI CAYNNNNRTG 2 cut(s) 213, 438
MspA1I CMGCKG 1 cut(s) 74
MspI CCGG 1 cut(s) 195
MspR9I CCNGG 3 cut(s) 551, 649, 1357
Mva1269I GAATGC 1 cut(s) 1289
MvaI CCWGG 3 cut(s) 551, 649, 1357
MwoI GCNNNNNNNGC 3 cut(s) 380, 619, 1152
NcoI CCATGG 1 cut(s) 388
NdeI CATATG 1 cut(s) 1086
NdeII GATC 3 cut(s) 111, 220, 977
NlaIV GGNNCC 1 cut(s) 829
NmeAIII GCCGAG 1 cut(s) 1049
NmuCI GTSAC 1 cut(s) 232
NsiI ATGCAT 1 cut(s) 823
NspI RCATGY 2 cut(s) 1326, 1481
NspV TTCGAA 1 cut(s) 741
PagI TCATGA 2 cut(s) 85, 208
PceI AGGCCT 1 cut(s) 1195
PctI GAATGC 1 cut(s) 1289
PdmI GAANNNNTTC 1 cut(s) 861
PfeI GAWTC 3 cut(s) 889, 1201, 1420
PflMI CCANNNNNTGG 2 cut(s) 556, 654
PkrI GCNGC 6 cut(s) 73, 76, 373, 469, 1154, 1270
PleI GAGTC 2 cut(s) 1352, 1445
PpsI GAGTC 2 cut(s) 1352, 1445
Psp6I CCWGG 3 cut(s) 549, 647, 1355
PspFI CCCAGC 1 cut(s) 571
PspGI CCWGG 3 cut(s) 549, 647, 1355
PspN4I GGNNCC 1 cut(s) 829
RsaI GTAC 2 cut(s) 365, 1454
RsaNI GTAC 2 cut(s) 364, 1453
RseI CAYNNNNRTG 2 cut(s) 213, 438
SaqAI TTAA 7 cut(s) 20, 42, 291, 438, 581, 773, 1034
SatI GCNGC 6 cut(s) 72, 75, 372, 468, 1153, 1269
Sau3AI GATC 3 cut(s) 111, 220, 977
SchI GAGTC 2 cut(s) 1353, 1446
ScrFI CCNGG 3 cut(s) 551, 649, 1357
SduI GDGCHC 1 cut(s) 1266
SfaNI GCATC 2 cut(s) 808, 1368
SfcI CTRYAG 1 cut(s) 78
SfuI TTCGAA 1 cut(s) 741
SmiMI CAYNNNNRTG 2 cut(s) 213, 438
Sse9I AATT 8 cut(s) 30, 202, 768, 783, 861, 948, 1401, 1407
SseBI AGGCCT 1 cut(s) 1195
SsiI CCGC 1 cut(s) 74
SspMI CTAG 4 cut(s) 131, 521, 608, 1133
StuI AGGCCT 1 cut(s) 1195
StyD4I CCNGG 3 cut(s) 549, 647, 1355
StyI CCWWGG 3 cut(s) 130, 388, 515
TaaI ACNGT 1 cut(s) 796
TaqI TCGA 6 cut(s) 114, 741, 804, 933, 1226, 1347
TasI AATT 8 cut(s) 30, 202, 768, 783, 861, 948, 1401, 1407
TatI WGTACW 1 cut(s) 1452
TauI GCSGC 1 cut(s) 77
TfiI GAWTC 3 cut(s) 889, 1201, 1420
Tru1I TTAA 7 cut(s) 20, 42, 291, 438, 581, 773, 1034
Tru9I TTAA 7 cut(s) 20, 42, 291, 438, 581, 773, 1034
TscAI CASTG 2 cut(s) 799, 859
TseFI GTSAC 1 cut(s) 232
TseI GCWGC 5 cut(s) 71, 371, 467, 1152, 1268
Tsp45I GTSAC 1 cut(s) 232
TspDTI ATGAA 9 cut(s) 423, 852, 902, 972, 980, 1025, 1071, 1307, 1500
TspRI CASTG 2 cut(s) 799, 859
Van91I CCANNNNNTGG 2 cut(s) 556, 654
XagI CCTNNNNNAGG 1 cut(s) 1454
XapI RAATTY 1 cut(s) 768
XceI RCATGY 2 cut(s) 1326, 1481
XmaJI CCTAGG 1 cut(s) 130
XmnI GAANNNNTTC 1 cut(s) 861
XspI CTAG 4 cut(s) 131, 521, 608, 1133
Zsp2I ATGCAT 1 cut(s) 823
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.