Rorug03G0295400

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000053
Physical Location & Seq
Reverse (-)
30441199 .. 30444763
3565 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0295400.1

Sequence Viewer

Length: 2598 bp
ATGGTACCTCCTAATGATCCTATTAGTCCAGTTTTCAAGGCGTTCCGGTCCGAGAGTTCTGGAAAATACTTTGACCTCTCTAATGGAAAGCTTAGTGTCAAAGGTGTTCCATTGCTCTCTGAAGTTCCAAGCAATGTAACTTTCAGGGACTTCCACTCAGCCTGCCAATCCTCTGATGCTCCACTTCCTCTGCTCCAACGGGTTCGTGCTTGGTCCCACAAGGGTGCCTTCCTGGGATTCAACAAGGAGGTTCCCTCAGATAGGCTGCTCAACTCCTTGGGGAAACTCAGTAACAGGGATTTCCTTAGCATCTTTAGGTTCAAGACATGGTGGTCTACCATGTGGGTGGGAAACTCTGGTTCAAACTTGCAAATGGAAACCCAATGGGTGCTCTTAGATGTCCCGGAGATCAAGTCCTATGTTATAATCATACCCATCATTGAAGCCAACTTCAGGTCTGCTCTTCATCCTGGCAGTGATGACCATGTCATGATCTGCGCCGAGAGTGGTTCTAGTCAAGTAAAAGCGTCGAATTTCGATGCCATTGCTTATGTTCATGCTTCTGACAATCCCTACAACTTGATGAAAGAAGCCTATAGTGCTGTTAGAGTCCATCTCAATAGTTTTAGGCTCTTGGAAGAGAAGACGGTCCCAAATCTAGTTAACAAATTTGGTTGGTGCACTTGGGATGCATTCTACTTGACTGTAGAACCTGTTGGTGTCTGGCATGGTGTGAAAGAATTTGTTGAAGGCGGTGTGTCCCCGCGGTTTCTCATAATTGATGATGGTTGGCAAAGCATCAATAAGGATGATGAGGACCTGAAGGAGGACACAAAAAATCTAGTTCTTGGTGGGACTCAAATGACTGCTAGGCTTCACAGTTTTGAGGAGAACAAAAAGTTCAGGAACTACAGAGGTGGCTCTATGTTGGGTCCTGATGCTCCTTCATTTGATCCCAAAAAGCCAAAACTGCTGATTGCCAAGGCAATTGAAATAGAGCATGCTGAGAAGGAACACGACAAGGCCCTTCATTCTGGAGTCACTGAGTTGTCTGTGTTCGAAAAAAAACTTCAGAAGCTGAAGACAGAGTTGAATGATATCATTGGTGAAGGAGAGAGCAGTGCTTCTAATGAATTCCGTGGAAGCAGTTCTGGCAGTGACAAAAACTATGGAATGAAGGCCTTCACAGGTGACTTGAGAACCAAGTTTAAAGGTTTGGATGACATATATGTATGGCATGCTCTTTGTGGTGCGTGGGGTGGTGTTAGGCCAGGTTCAACTCATCTCAGTTCAAAGATCATTCCTTGCAAAGTCTCACCTGGACTAGACGGGACGATGAATGATCTGGCTGTGGTGAAAATCATTGAGGGCGGGATTGGGCTTGTTCATCCTGATCAAGCTGATAGTTTCTATGACTCCATGCACTCTTACCTTTCTGAAGTTGGTATCACTGGAGTCAAAGTGGATGTAATTCATACACTTGAATATGTATCCGAGGAATACGGAGGCCGTGTGGAGCTTGCAAAGGCTTATTACAAGGGGCTGACTCATTCTCTTCAGAAGAACTTCAATGGGACTGGACTTATTGCTAGCATGCAGCAATGCAATGACTTCTTCTTTCTTGGCACAAAGCAAATCTCTATTGGAAGAGTCGGTGACGATTTTTGGTTTCAGGATCCGAATGGAGATCCAATGGGAGTCTACTGGTTACAAGGAGTTCATATGATCCACTGCGCTTACAACAGCATGTGGATGGGCCAGATGATACAGCCTGATTGGGACATGTTTCAATCAGACCATTTATGTGCCAAATTTCATGCAGGATCAAGAGCAATCTGTGGGGGTCCGGTTTATGTAAGTGACTCAGTTTCAGGGCATGATTTCGATCTCATTAAGAAGCTTGTTTACCCGGATGGTACCATTCCCAAGTGCCAGCATTTCGCTCTCCCAACCAGGGACTGCCTCTTCAAAAACCCTTTATTTGATGATAAGACTGTCCTGAAAATTTGGAACTTTAACAAGTATGGCGGTGTCATTGGAGCTTTCAATTGTCAAGGGGCTGGTTGGGACCCCAAGGAACAGAGGATCAAAGGCCACTCAGAGTGCTACAAGCCAATCTCTTGTTCATTACATGTTCGTGAGATTGAATGGGACCAAAAGTCTGAAGCAGCCCATTTGGGCGAGGCTGAAGAGTACGTTGTCTACCTCAGCGAGGTTGATGAGCTCCGCCTGATGACCCCAAAGTCCGATGCAATTCAAATCGTCATCCAGCCATCTTCATTTGAGATTTTGAGCTTTGTGCCGGTTAGAAAGCTAGGCCACAGCATTAAGTTCGCGCCAATTGGACTTACAAACATGTTCAACTGTGGAGGCACACTCCAAGAGTTGGAGTTCAGAAGTACCGCGGTTGAGTTTAGTGCCATGATCAAAGTCAAGGGTGGAGGCATGTTCCTGGCCTACTCAAGTGAGTCACCAAAGAAATGTTGTTTGAATGGTGCTGAGGTAGCTTTTGAGTGGTCTGCTGATGGCAAATTGAACTTGAGTGTTCCTTGGGTTGAACAAGCTGCTGGCATTTCTGATGTGCTTTTTGCATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

865

Amino Acids

96.25

Weight (kDa)

5.91

Isoelectric Point (pI)

32.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 24 - 848 0.0 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 425
AasI GACNNNNNNGTC 2 cut(s) 331, 2242
Acc65I GGTACC 2 cut(s) 4, 1916
AccB1I GGYRCC 3 cut(s) 4, 224, 1916
AccB7I CCANNNNNTGG 1 cut(s) 2386
AccI GTMKAC 3 cut(s) 335, 1701, 2202
AccII CGCG 3 cut(s) 766, 2336, 2405
AciI CCGC 8 cut(s) 753, 764, 766, 1371, 2028, 2227, 2403, 2405
AclWI GGATC 8 cut(s) 11, 947, 1670, 1682, 1683, 1720, 1831, 2093
AcsI RAATTY 6 cut(s) 532, 668, 740, 1133, 1811, 2004
AcuI CTGAAG 9 cut(s) 141, 436, 842, 1055, 1100, 1458, 1541, 2184, 2208
AfaI GTAC 4 cut(s) 6, 1918, 2195, 2401
AfiI CCNNNNNNNGG 6 cut(s) 261, 453, 826, 1954, 2212, 2386
AflIII ACRYGT 3 cut(s) 1782, 2131, 2355
AhdI GACNNNNNGTC 1 cut(s) 2158
AjnI CCWGG 6 cut(s) 231, 469, 1270, 1318, 1952, 2451
AjuI GAANNNNNNNTTGG 2 cut(s) 2107, 2139
AleI CACNNNNGTG 1 cut(s) 222
Alw21I GWGCWC 3 cut(s) 393, 683, 2226
Alw26I GTCTC 1 cut(s) 1318
Alw44I GTGCAC 1 cut(s) 679
AlwI GGATC 8 cut(s) 11, 947, 1670, 1682, 1683, 1720, 1831, 2093
AlwNI CAGNNNCTG 2 cut(s) 1078, 1959
AoxI GGCC 8 cut(s) 1023, 1179, 1268, 1507, 1756, 2092, 2317, 2454
ApaLI GTGCAC 1 cut(s) 679
ApeKI GCWGC 4 cut(s) 265, 1597, 2168, 2564
ApoI RAATTY 6 cut(s) 532, 668, 740, 1133, 1811, 2004
Asp700I GAANNNNTTC 3 cut(s) 1147, 1181, 1565
Asp718I GGTACC 2 cut(s) 4, 1916
AspLEI GCGC 3 cut(s) 500, 1736, 2338
AsuC2I CCSGG 2 cut(s) 404, 1910
AsuHPI GGTGA 6 cut(s) 1118, 1202, 1308, 1366, 1667, 2463
AsuII TTCGAA 1 cut(s) 1059
AsuNHI GCTAGC 1 cut(s) 1589
AvaII GGWCC 8 cut(s) 48, 213, 649, 817, 932, 1844, 2068, 2152
BaeGI GKGCMC 1 cut(s) 683
BamHI GGATCC 1 cut(s) 1675
BanI GGYRCC 3 cut(s) 4, 224, 1916
BanII GRGCYC 1 cut(s) 2226
BarI GAAGNNNNNNTAC 2 cut(s) 1889, 1921
BbsI GAAGAC 2 cut(s) 650, 1088
Bbv12I GWGCWC 3 cut(s) 393, 683, 2226
BbvCI CCTCAGC 2 cut(s) 2207, 2499
BbvI GCAGC 4 cut(s) 252, 1609, 2180, 2551
BccI CCATC 7 cut(s) 443, 621, 779, 1747, 1907, 2281, 2519
BceAI ACGGC 1 cut(s) 1494
BcgI CGANNNNNNTGC 4 cut(s) 527, 561, 2314, 2348
BciT130I CCWGG 6 cut(s) 233, 471, 1272, 1320, 1954, 2453
BciVI GTATCC 1 cut(s) 1501
BclI TGATCA 2 cut(s) 1393, 2424
BcnI CCSGG 2 cut(s) 404, 1910
BcoDI GTCTC 1 cut(s) 1318
BfaI CTAG 7 cut(s) 513, 659, 842, 870, 1325, 1590, 2315
BfmI CTRYAG 3 cut(s) 595, 705, 910
BfuI GTATCC 1 cut(s) 1501
BisI GCNGC 4 cut(s) 266, 1598, 2169, 2565
BlsI GCNGC 4 cut(s) 267, 1599, 2170, 2566
Bme1390I CCNGG 8 cut(s) 233, 404, 471, 1272, 1320, 1910, 1954, 2453
Bme18I GGWCC 8 cut(s) 48, 213, 649, 817, 932, 1844, 2068, 2152
BmeRI GACNNNNNGTC 1 cut(s) 2158
BmrFI CCNGG 8 cut(s) 233, 404, 471, 1272, 1320, 1910, 1954, 2453
BmsI GCATC 7 cut(s) 166, 318, 529, 679, 807, 928, 2239
BmtI GCTAGC 1 cut(s) 1593
BpiI GAAGAC 2 cut(s) 650, 1088
BplI GAGNNNNNCTC 6 cut(s) 239, 271, 600, 632, 2361, 2393
BpmI CTGGAG 2 cut(s) 1056, 1473
Bpu10I CCTNAGC 3 cut(s) 305, 2207, 2499
Bpu14I TTCGAA 1 cut(s) 1059
BpuEI CTTGAG 3 cut(s) 1216, 2446, 2560
BpuMI CCSGG 2 cut(s) 404, 1910
BsaBI GATNNNNATC 1 cut(s) 1884
BsaWI WCCGGW 2 cut(s) 45, 1846
BsaXI ACNNNNNCTCC 6 cut(s) 398, 428, 1446, 1476, 1497, 1527
Bsc4I CCNNNNNNNGG 6 cut(s) 261, 453, 826, 1954, 2212, 2386
Bse118I RCCGGY 1 cut(s) 2302
Bse1I ACTGG 4 cut(s) 29, 1456, 1582, 1709
Bse3DI GCAATG 5 cut(s) 110, 139, 543, 1607, 1612
Bse8I GATNNNNATC 1 cut(s) 1884
BseBI CCWGG 6 cut(s) 233, 471, 1272, 1320, 1954, 2453
BseGI GGATG 9 cut(s) 466, 694, 814, 1225, 1387, 1471, 1758, 1918, 2265
BseJI GATNNNNATC 1 cut(s) 1884
BseLI CCNNNNNNNGG 6 cut(s) 261, 453, 826, 1954, 2212, 2386
BseMI GCAATG 5 cut(s) 110, 139, 543, 1607, 1612
BseNI ACTGG 4 cut(s) 29, 1456, 1582, 1709
BseRI GAGGAG 1 cut(s) 902
BseSI GKGCMC 1 cut(s) 683
BseXI GCAGC 4 cut(s) 252, 1609, 2180, 2551
Bsh1236I CGCG 3 cut(s) 766, 2336, 2405
BshFI GGCC 8 cut(s) 1025, 1181, 1270, 1509, 1758, 2094, 2319, 2456
BshNI GGYRCC 3 cut(s) 4, 224, 1916
BsiHKAI GWGCWC 3 cut(s) 393, 683, 2226
BsiSI CCGG 5 cut(s) 46, 404, 1847, 1910, 2303
BslI CCNNNNNNNGG 6 cut(s) 261, 453, 826, 1954, 2212, 2386
BsmAI GTCTC 1 cut(s) 1318
BsmI GAATGC 1 cut(s) 692
BsnI GGCC 8 cut(s) 1025, 1181, 1270, 1509, 1758, 2094, 2319, 2456
Bsp119I TTCGAA 1 cut(s) 1059
Bsp1286I GDGCHC 3 cut(s) 393, 683, 2226
BspACI CCGC 8 cut(s) 753, 764, 766, 1371, 2028, 2227, 2403, 2405
BspANI GGCC 8 cut(s) 1025, 1181, 1270, 1509, 1758, 2094, 2319, 2456
BspFNI CGCG 3 cut(s) 766, 2336, 2405
BspHI TCATGA 1 cut(s) 489
BspOI GCTAGC 1 cut(s) 1593
BspPI GGATC 8 cut(s) 11, 947, 1670, 1682, 1683, 1720, 1831, 2093
BspQI GCTCTTC 1 cut(s) 468
BspT104I TTCGAA 1 cut(s) 1059
BspT107I GGYRCC 3 cut(s) 4, 224, 1916
BsrDI GCAATG 5 cut(s) 110, 139, 543, 1607, 1612
BsrFI RCCGGY 1 cut(s) 2302
BsrI ACTGG 4 cut(s) 29, 1456, 1582, 1709
BssAI RCCGGY 1 cut(s) 2302
BssT1I CCWWGG 4 cut(s) 276, 981, 2073, 2549
Bst2UI CCWGG 6 cut(s) 233, 471, 1272, 1320, 1954, 2453
Bst4CI ACNGT 5 cut(s) 649, 706, 881, 1996, 2366
Bst6I CTCTTC 6 cut(s) 468, 633, 1560, 1642, 1970, 2184
BstBI TTCGAA 1 cut(s) 1059
BstC8I GCNNGC 8 cut(s) 163, 1002, 1239, 1521, 1591, 1595, 1934, 2569
BstDSI CCRYGG 3 cut(s) 764, 1138, 2403
BstENI CCTNNNNNAGG 3 cut(s) 259, 824, 2210
BstF5I GGATG 9 cut(s) 466, 694, 814, 1225, 1387, 1471, 1758, 1918, 2265
BstFNI CGCG 3 cut(s) 766, 2336, 2405
BstHHI GCGC 3 cut(s) 500, 1736, 2338
BstMAI GTCTC 1 cut(s) 1318
BstMWI GCNNNNNNNGC 4 cut(s) 599, 970, 1152, 2504
BstNI CCWGG 6 cut(s) 233, 471, 1272, 1320, 1954, 2453
BstNSI RCATGY 8 cut(s) 1004, 1241, 1597, 1750, 1786, 2135, 2359, 2449
BstSCI CCNGG 8 cut(s) 231, 402, 469, 1270, 1318, 1908, 1952, 2451
BstSFI CTRYAG 3 cut(s) 595, 705, 910
BstSLI GKGCMC 1 cut(s) 683
BstUI CGCG 3 cut(s) 766, 2336, 2405
BstV1I GCAGC 4 cut(s) 252, 1609, 2180, 2551
BstV2I GAAGAC 2 cut(s) 650, 1088
BstX2I RGATCY 2 cut(s) 1675, 1687
BstXI CCANNNNNNTGG 1 cut(s) 346
BstYI RGATCY 2 cut(s) 1675, 1687
BsuI GTATCC 1 cut(s) 1501
BsuRI GGCC 8 cut(s) 1025, 1181, 1270, 1509, 1758, 2094, 2319, 2456
BtgI CCRYGG 3 cut(s) 764, 1138, 2403
BtsCI GGATG 9 cut(s) 466, 694, 814, 1225, 1387, 1471, 1758, 1918, 2265
BtsI GCAGTG 4 cut(s) 481, 1126, 1162, 1729
BtsIMutI CAGTG 6 cut(s) 481, 1041, 1126, 1162, 1449, 1729
Cac8I GCNNGC 8 cut(s) 163, 1002, 1239, 1521, 1591, 1595, 1934, 2569
CaiI CAGNNNCTG 2 cut(s) 1078, 1959
CciI TCATGA 1 cut(s) 489
CfoI GCGC 3 cut(s) 500, 1736, 2338
Cfr10I RCCGGY 1 cut(s) 2302
Cfr42I CCGCGG 2 cut(s) 767, 2406
CpoI CGGWCCG 1 cut(s) 48
CseI GACGC 1 cut(s) 516
Csp6I GTAC 4 cut(s) 5, 1917, 2194, 2400
CspI CGGWCCG 1 cut(s) 48
CviQI GTAC 4 cut(s) 5, 1917, 2194, 2400
DraI TTTAAA 1 cut(s) 1210
DrdI GACNNNNNNGTC 2 cut(s) 331, 2242
DriI GACNNNNNGTC 1 cut(s) 2158
DseDI GACNNNNNNGTC 2 cut(s) 331, 2242
Eam1104I CTCTTC 6 cut(s) 468, 633, 1560, 1642, 1970, 2184
Eam1105I GACNNNNNGTC 1 cut(s) 2158
EarI CTCTTC 6 cut(s) 468, 633, 1560, 1642, 1970, 2184
EciI GGCGGA 1 cut(s) 2216
Ecl136II GAGCTC 1 cut(s) 2224
Eco130I CCWWGG 4 cut(s) 276, 981, 2073, 2549
Eco147I AGGCCT 1 cut(s) 1181
Eco24I GRGCYC 1 cut(s) 2226
Eco32I GATATC 1 cut(s) 1099
Eco47I GGWCC 8 cut(s) 48, 213, 649, 817, 932, 1844, 2068, 2152
Eco53kI GAGCTC 1 cut(s) 2224
Eco57I CTGAAG 9 cut(s) 141, 436, 842, 1055, 1100, 1458, 1541, 2184, 2208
EcoICRI GAGCTC 1 cut(s) 2224
EcoNI CCTNNNNNAGG 3 cut(s) 259, 824, 2210
EcoO109I RGGNCCY 4 cut(s) 817, 932, 1024, 2068
EcoRI GAATTC 1 cut(s) 1133
EcoRII CCWGG 6 cut(s) 231, 469, 1270, 1318, 1952, 2451
EcoRV GATATC 1 cut(s) 1099
EcoT14I CCWWGG 4 cut(s) 276, 981, 2073, 2549
EcoT22I ATGCAT 1 cut(s) 694
EcoT38I GRGCYC 1 cut(s) 2226
ErhI CCWWGG 4 cut(s) 276, 981, 2073, 2549
FalI AAGNNNNNCTT 2 cut(s) 212, 244
FauI CCCGC 2 cut(s) 771, 1364
FauNDI CATATG 1 cut(s) 1722
FbaI TGATCA 2 cut(s) 1393, 2424
FblI GTMKAC 3 cut(s) 335, 1701, 2202
Fnu4HI GCNGC 4 cut(s) 266, 1598, 2169, 2565
FokI GGATG 9 cut(s) 453, 701, 821, 1232, 1374, 1478, 1765, 1925, 2252
FriOI GRGCYC 1 cut(s) 2226
Fsp4HI GCNGC 4 cut(s) 266, 1598, 2169, 2565
FspBI CTAG 7 cut(s) 513, 659, 842, 870, 1325, 1590, 2315
GlaI GCGC 3 cut(s) 499, 1735, 2337
GluI GCNGC 4 cut(s) 266, 1598, 2169, 2565
GsuI CTGGAG 2 cut(s) 1056, 1473
HaeIII GGCC 8 cut(s) 1025, 1181, 1270, 1509, 1758, 2094, 2319, 2456
HapII CCGG 5 cut(s) 46, 404, 1847, 1910, 2303
HgaI GACGC 1 cut(s) 516
HhaI GCGC 3 cut(s) 500, 1736, 2338
Hin6I GCGC 3 cut(s) 498, 1734, 2336
HinP1I GCGC 3 cut(s) 498, 1734, 2336
HincII GTYRAC 1 cut(s) 664
HindII GTYRAC 1 cut(s) 664
HindIII AAGCTT 2 cut(s) 89, 1898
HpaI GTTAAC 1 cut(s) 664
HpaII CCGG 5 cut(s) 46, 404, 1847, 1910, 2303
HphI GGTGA 6 cut(s) 1118, 1202, 1308, 1366, 1667, 2463
Hpy166II GTNNAC 6 cut(s) 336, 664, 681, 1702, 1906, 2203
Hpy8I GTNNAC 6 cut(s) 336, 664, 681, 1702, 1906, 2203
Hpy99I CGWCG 1 cut(s) 532
HpyAV CCTTC 9 cut(s) 238, 743, 817, 954, 1003, 1037, 1103, 1171, 1192
HpyCH4III ACNGT 5 cut(s) 649, 706, 881, 1996, 2366
HpyCH4IV ACGT 1 cut(s) 2196
HpyF10VI GCNNNNNNNGC 4 cut(s) 599, 970, 1152, 2504
HpySE526I ACGT 1 cut(s) 2196
HspAI GCGC 3 cut(s) 498, 1734, 2336
KflI GGGWCCC 1 cut(s) 2068
KpnI GGTACC 2 cut(s) 8, 1920
Ksp22I TGATCA 2 cut(s) 1393, 2424
KspAI GTTAAC 1 cut(s) 664
KspI CCGCGG 2 cut(s) 767, 2406
LguI GCTCTTC 1 cut(s) 468
LmnI GCTCC 6 cut(s) 184, 198, 946, 1516, 2039, 2229
Lsp1109I GCAGC 4 cut(s) 252, 1609, 2180, 2551
LweI GCATC 7 cut(s) 166, 318, 529, 679, 807, 928, 2239
MaeI CTAG 7 cut(s) 513, 659, 842, 870, 1325, 1590, 2315
MaeII ACGT 1 cut(s) 2196
MaeIII GTNAC 9 cut(s) 136, 290, 1039, 1157, 1190, 1655, 1707, 1859, 2469
MfeI CAATTG 3 cut(s) 987, 2047, 2340
MflI RGATCY 2 cut(s) 1675, 1687
MhlI GDGCHC 3 cut(s) 393, 683, 2226
MmeI TCCRAC 2 cut(s) 220, 2367
Mph1103I ATGCAT 1 cut(s) 694
MroXI GAANNNNTTC 3 cut(s) 1147, 1181, 1565
MseI TTAA 5 cut(s) 663, 1209, 1893, 2016, 2328
MslI CAYNNNNRTG 5 cut(s) 222, 344, 1751, 1803, 2136
MspA1I CMGCKG 2 cut(s) 766, 2405
MspI CCGG 5 cut(s) 46, 404, 1847, 1910, 2303
MspR9I CCNGG 8 cut(s) 233, 404, 471, 1272, 1320, 1910, 1954, 2453
MunI CAATTG 3 cut(s) 987, 2047, 2340
Mva1269I GAATGC 1 cut(s) 692
MvaI CCWGG 6 cut(s) 233, 471, 1272, 1320, 1954, 2453
MvnI CGCG 3 cut(s) 766, 2336, 2405
MwoI GCNNNNNNNGC 4 cut(s) 599, 970, 1152, 2504
NciI CCSGG 2 cut(s) 404, 1910
NdeI CATATG 1 cut(s) 1722
NheI GCTAGC 1 cut(s) 1589
NmeAIII GCCGAG 1 cut(s) 526
NmuCI GTSAC 6 cut(s) 1039, 1157, 1190, 1655, 1859, 2469
NsiI ATGCAT 1 cut(s) 694
NspI RCATGY 8 cut(s) 1004, 1241, 1597, 1750, 1786, 2135, 2359, 2449
NspV TTCGAA 1 cut(s) 1059
OliI CACNNNNGTG 1 cut(s) 222
PaeI GCATGC 3 cut(s) 1004, 1241, 1597
PagI TCATGA 1 cut(s) 489
PceI AGGCCT 1 cut(s) 1181
PciI ACATGT 3 cut(s) 1782, 2131, 2355
PciSI GCTCTTC 1 cut(s) 468
PctI GAATGC 1 cut(s) 692
PdmI GAANNNNTTC 3 cut(s) 1147, 1181, 1565
PfeI GAWTC 1 cut(s) 237
PflFI GACNNNGTC 1 cut(s) 485
PflMI CCANNNNNTGG 1 cut(s) 2386
PfoI TCCNGGA 1 cut(s) 402
PkrI GCNGC 4 cut(s) 267, 1599, 2170, 2566
PpuMI RGGWCCY 3 cut(s) 817, 932, 2068
PscI ACATGT 3 cut(s) 1782, 2131, 2355
PsiI TTATAA 1 cut(s) 425
Psp124BI GAGCTC 1 cut(s) 2226
Psp5II RGGWCCY 3 cut(s) 817, 932, 2068
Psp6I CCWGG 6 cut(s) 231, 469, 1270, 1318, 1952, 2451
PspGI CCWGG 6 cut(s) 231, 469, 1270, 1318, 1952, 2451
PspPPI RGGWCCY 3 cut(s) 817, 932, 2068
PstNI CAGNNNCTG 2 cut(s) 1078, 1959
PsuI RGATCY 2 cut(s) 1675, 1687
PsyI GACNNNGTC 1 cut(s) 485
RsaI GTAC 4 cut(s) 6, 1918, 2195, 2401
RsaNI GTAC 4 cut(s) 5, 1917, 2194, 2400
RseI CAYNNNNRTG 5 cut(s) 222, 344, 1751, 1803, 2136
Rsr2I CGGWCCG 1 cut(s) 48
RsrII CGGWCCG 1 cut(s) 48
SacI GAGCTC 1 cut(s) 2226
SacII CCGCGG 2 cut(s) 767, 2406
SapI GCTCTTC 1 cut(s) 468
SaqAI TTAA 5 cut(s) 663, 1209, 1893, 2016, 2328
SatI GCNGC 4 cut(s) 266, 1598, 2169, 2565
ScrFI CCNGG 8 cut(s) 233, 404, 471, 1272, 1320, 1910, 1954, 2453
SduI GDGCHC 3 cut(s) 393, 683, 2226
SfaNI GCATC 7 cut(s) 166, 318, 529, 679, 807, 928, 2239
SfcI CTRYAG 3 cut(s) 595, 705, 910
Sfr303I CCGCGG 2 cut(s) 767, 2406
SfuI TTCGAA 1 cut(s) 1059
SgrBI CCGCGG 2 cut(s) 767, 2406
SinI GGWCC 8 cut(s) 48, 213, 649, 817, 932, 1844, 2068, 2152
SmiMI CAYNNNNRTG 5 cut(s) 222, 344, 1751, 1803, 2136
SmlI CTYRAG 3 cut(s) 1195, 2461, 2539
SmoI CTYRAG 3 cut(s) 1195, 2461, 2539
SphI GCATGC 3 cut(s) 1004, 1241, 1597
SseBI AGGCCT 1 cut(s) 1181
SsiI CCGC 8 cut(s) 753, 764, 766, 1371, 2028, 2227, 2403, 2405
SspMI CTAG 7 cut(s) 513, 659, 842, 870, 1325, 1590, 2315
SstI GAGCTC 1 cut(s) 2226
StuI AGGCCT 1 cut(s) 1181
StyD4I CCNGG 8 cut(s) 231, 402, 469, 1270, 1318, 1908, 1952, 2451
StyI CCWWGG 4 cut(s) 276, 981, 2073, 2549
TaaI ACNGT 5 cut(s) 649, 706, 881, 1996, 2366
TaiI ACGT 1 cut(s) 2199
TaqI TCGA 4 cut(s) 530, 537, 1059, 1884
TfiI GAWTC 1 cut(s) 237
Tru1I TTAA 5 cut(s) 663, 1209, 1893, 2016, 2328
Tru9I TTAA 5 cut(s) 663, 1209, 1893, 2016, 2328
TscAI CASTG 6 cut(s) 481, 1048, 1126, 1162, 1456, 1736
TseFI GTSAC 6 cut(s) 1039, 1157, 1190, 1655, 1859, 2469
TseI GCWGC 4 cut(s) 265, 1597, 2168, 2564
Tsp45I GTSAC 6 cut(s) 1039, 1157, 1190, 1655, 1859, 2469
TspGWI ACGGA 2 cut(s) 1127, 1518
TspRI CASTG 6 cut(s) 481, 1048, 1126, 1162, 1456, 1736
Tth111I GACNNNGTC 1 cut(s) 485
Van91I CCANNNNNTGG 1 cut(s) 2386
VneI GTGCAC 1 cut(s) 679
VpaK11BI GGWCC 8 cut(s) 48, 213, 649, 817, 932, 1844, 2068, 2152
XagI CCTNNNNNAGG 3 cut(s) 259, 824, 2210
XapI RAATTY 6 cut(s) 532, 668, 740, 1133, 1811, 2004
XceI RCATGY 8 cut(s) 1004, 1241, 1597, 1750, 1786, 2135, 2359, 2449
XmiI GTMKAC 3 cut(s) 335, 1701, 2202
XmnI GAANNNNTTC 3 cut(s) 1147, 1181, 1565
XspI CTAG 7 cut(s) 513, 659, 842, 870, 1325, 1590, 2315
Zsp2I ATGCAT 1 cut(s) 694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.