RchiOBHm_Chr6g0245691

(-)-germacrene D synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000053
Physical Location & Seq
Forward (+)
1462710 .. 1464790
2081 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22020

Sequence Viewer

Length: 765 bp
ATGGCATCTATTATTGATGACATTTATGATGCTCATGGCACACTCGAAGAATTAGAGCTTTTTACTAAAGCTATTCAGAGGTGGGACGTCTGTGTCATAGATCTGTTACCAGATTATATGAAAGTCTGTTATAAGGCACTATTAGAAGTTTATACTGAAATTGAAGAACAGCTTGCAAAAGAAGGAAAGTTGTATCAAATCCAGTACGCCAGAGAACCTATGAAAAAGCTATCTGAAAATTACTTCCTTGAAGCTAAATGGTTGCACCAGAAGCACATACCAACAATGGATGATTATATGTCCCTAGCATTAATGACCACAGGCTACCCCCTGCTAATAACAACAGCCTTTGTTGGAATGGGAAATATTGCTACACAAGACTCCTTCGATTGGTTGGCCACTTATCCCAAGGCTGTAAAAGGGGCTGCAATAATTTGCCGACTCATGGATGACATAGCGGACCATAAGTTTGAGCAAAAGAGAGGACAAGTTACCTTAGCTGTAGAGTGCTACATGAAAGAATATGGTGCCACAGAAGAAGAAGCAATAATCGGATTGCGTAGAGAAGTGAGTGATGCATGGAAGGACATAAATGAATCGTTCCTCCTCCCTAATGTTCCTCCGAGGCCATTGCTAACTCGAATTATTAACTTTGCATGTATCATGGATGTTGCATACAAGTACGCAGATGGTTACACTACTCATCATGATACTGTGCTTAAGGATTTGATAGTTTCTACACTTGTCCAACCCGTGCTTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

254

Amino Acids

29.13

Weight (kDa)

4.89

Isoelectric Point (pI)

44.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth_C PF03936 1 - 196 8.3e-77 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 2 - 196 1.6e-58 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 132
AasI GACNNNNNNGTC 1 cut(s) 92
AatII GACGTC 1 cut(s) 90
AccB1I GGYRCC 1 cut(s) 527
AciI CCGC 1 cut(s) 458
AcoI YGGCCR 1 cut(s) 396
AcyI GRCGYC 1 cut(s) 87
AfaI GTAC 2 cut(s) 206, 683
AfiI CCNNNNNNNGG 2 cut(s) 390, 445
AflII CTTAAG 1 cut(s) 719
AgsI TTSAA 2 cut(s) 164, 251
AluBI AGCT 6 cut(s) 58, 71, 172, 229, 254, 500
AluI AGCT 6 cut(s) 58, 71, 172, 229, 254, 500
AoxI GGCC 2 cut(s) 396, 626
ApeKI GCWGC 1 cut(s) 425
ArsI GACNNNNNNTTYG 2 cut(s) 452, 484
AseI ATTAAT 1 cut(s) 311
AspS9I GGNCC 1 cut(s) 460
AvaII GGWCC 1 cut(s) 460
BalI TGGCCA 1 cut(s) 398
BanI GGYRCC 1 cut(s) 527
BbvI GCAGC 1 cut(s) 412
BccI CCATC 1 cut(s) 683
BcgI CGANNNNNNTGC 2 cut(s) 613, 647
BfaI CTAG 1 cut(s) 305
BfmI CTRYAG 1 cut(s) 501
BfrI CTTAAG 1 cut(s) 719
BglII AGATCT 1 cut(s) 100
BisI GCNGC 1 cut(s) 426
BlsI GCNGC 1 cut(s) 427
Bme18I GGWCC 1 cut(s) 460
BmgT120I GGNCC 1 cut(s) 460
BmiI GGNNCC 1 cut(s) 529
BmsI GCATC 3 cut(s) 14, 19, 565
Bpu10I CCTNAGC 1 cut(s) 496
BsaHI GRCGYC 1 cut(s) 87
BsaJI CCNNGG 2 cut(s) 408, 623
Bsc4I CCNNNNNNNGG 2 cut(s) 390, 445
Bse1I ACTGG 1 cut(s) 202
Bse3DI GCAATG 1 cut(s) 629
BseDI CCNNGG 2 cut(s) 408, 623
BseGI GGATG 3 cut(s) 295, 454, 673
BseLI CCNNNNNNNGG 2 cut(s) 390, 445
BseMI GCAATG 1 cut(s) 629
BseNI ACTGG 1 cut(s) 202
BseRI GAGGAG 1 cut(s) 596
BseXI GCAGC 1 cut(s) 412
BshFI GGCC 2 cut(s) 398, 628
BshNI GGYRCC 1 cut(s) 527
BslFI GGGAC 2 cut(s) 98, 286
BslI CCNNNNNNNGG 2 cut(s) 390, 445
BsmFI GGGAC 2 cut(s) 98, 286
BsnI GGCC 2 cut(s) 398, 628
Bsp143I GATC 1 cut(s) 100
BspACI CCGC 1 cut(s) 458
BspANI GGCC 2 cut(s) 398, 628
BspHI TCATGA 1 cut(s) 706
BspLI GGNNCC 1 cut(s) 529
BspT107I GGYRCC 1 cut(s) 527
BspTI CTTAAG 1 cut(s) 719
BsrDI GCAATG 1 cut(s) 629
BsrI ACTGG 1 cut(s) 202
BssECI CCNNGG 2 cut(s) 408, 623
BssMI GATC 1 cut(s) 100
BssNI GRCGYC 1 cut(s) 87
BssT1I CCWWGG 1 cut(s) 408
Bst4CI ACNGT 1 cut(s) 715
BstACI GRCGYC 1 cut(s) 87
BstAFI CTTAAG 1 cut(s) 719
BstC8I GCNNGC 1 cut(s) 174
BstDEI CTNAG 1 cut(s) 496
BstF5I GGATG 3 cut(s) 295, 454, 673
BstKTI GATC 1 cut(s) 103
BstMBI GATC 1 cut(s) 100
BstMWI GCNNNNNNNGC 1 cut(s) 271
BstNSI RCATGY 1 cut(s) 660
BstSFI CTRYAG 1 cut(s) 501
BstV1I GCAGC 1 cut(s) 412
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
BsuRI GGCC 2 cut(s) 398, 628
BtsCI GGATG 3 cut(s) 295, 454, 673
Cac8I GCNNGC 1 cut(s) 174
CciI TCATGA 1 cut(s) 706
Cfr13I GGNCC 1 cut(s) 460
Csp6I GTAC 2 cut(s) 205, 682
CviAII CATG 7 cut(s) 35, 445, 514, 579, 657, 664, 707
CviQI GTAC 2 cut(s) 205, 682
DdeI CTNAG 1 cut(s) 496
DpnI GATC 1 cut(s) 102
DpnII GATC 1 cut(s) 100
DrdI GACNNNNNNGTC 1 cut(s) 92
DseDI GACNNNNNNGTC 1 cut(s) 92
EaeI YGGCCR 1 cut(s) 396
Eco130I CCWWGG 1 cut(s) 408
Eco47I GGWCC 1 cut(s) 460
EcoT14I CCWWGG 1 cut(s) 408
EcoT22I ATGCAT 1 cut(s) 580
ErhI CCWWGG 1 cut(s) 408
FaeI CATG 7 cut(s) 38, 448, 517, 582, 660, 667, 710
FalI AAGNNNNNCTT 2 cut(s) 156, 188
FaqI GGGAC 2 cut(s) 98, 286
FatI CATG 7 cut(s) 34, 444, 513, 578, 656, 663, 706
Fnu4HI GCNGC 1 cut(s) 426
FokI GGATG 3 cut(s) 302, 461, 680
Fsp4HI GCNGC 1 cut(s) 426
FspBI CTAG 1 cut(s) 305
GluI GCNGC 1 cut(s) 426
HaeIII GGCC 2 cut(s) 398, 628
Hin1I GRCGYC 1 cut(s) 87
Hin1II CATG 7 cut(s) 38, 448, 517, 582, 660, 667, 710
HinfI GANTC 3 cut(s) 380, 441, 596
Hpy188I TCNGA 4 cut(s) 78, 235, 554, 624
Hpy188III TCNNGA 1 cut(s) 707
HpyAV CCTTC 3 cut(s) 176, 394, 577
HpyCH4III ACNGT 1 cut(s) 715
HpyCH4IV ACGT 1 cut(s) 87
HpyCH4V TGCA 6 cut(s) 176, 265, 428, 578, 656, 674
HpyF10VI GCNNNNNNNGC 1 cut(s) 271
HpyF3I CTNAG 1 cut(s) 496
HpySE526I ACGT 1 cut(s) 87
Hsp92I GRCGYC 1 cut(s) 87
Hsp92II CATG 7 cut(s) 38, 448, 517, 582, 660, 667, 710
Kzo9I GATC 1 cut(s) 100
LpnPI CCDG 6 cut(s) 123, 215, 223, 281, 306, 344
Lsp1109I GCAGC 1 cut(s) 412
LweI GCATC 3 cut(s) 14, 19, 565
MaeI CTAG 1 cut(s) 305
MaeII ACGT 1 cut(s) 87
MaeIII GTNAC 3 cut(s) 105, 490, 692
MalI GATC 1 cut(s) 102
MboI GATC 1 cut(s) 100
MboII GAAGA 4 cut(s) 59, 176, 548, 551
MflI RGATCY 1 cut(s) 100
MlsI TGGCCA 1 cut(s) 398
MluCI AATT 5 cut(s) 50, 159, 238, 432, 642
MluNI TGGCCA 1 cut(s) 398
MlyI GAGTC 2 cut(s) 374, 435
MmeI TCCRAC 1 cut(s) 334
MnlI CCTC 6 cut(s) 72, 476, 614, 617, 618, 630
Mox20I TGGCCA 1 cut(s) 398
Mph1103I ATGCAT 1 cut(s) 580
MscI TGGCCA 1 cut(s) 398
MseI TTAA 3 cut(s) 311, 648, 720
Msp20I TGGCCA 1 cut(s) 398
MspCI CTTAAG 1 cut(s) 719
MwoI GCNNNNNNNGC 1 cut(s) 271
NdeII GATC 1 cut(s) 100
NlaIII CATG 7 cut(s) 38, 448, 517, 582, 660, 667, 710
NlaIV GGNNCC 1 cut(s) 529
NsiI ATGCAT 1 cut(s) 580
NspI RCATGY 1 cut(s) 660
PagI TCATGA 1 cut(s) 706
PfeI GAWTC 1 cut(s) 596
PkrI GCNGC 1 cut(s) 427
PleI GAGTC 2 cut(s) 374, 435
PpsI GAGTC 2 cut(s) 374, 435
PshBI ATTAAT 1 cut(s) 311
PsiI TTATAA 1 cut(s) 132
PspN4I GGNNCC 1 cut(s) 529
PspPI GGNCC 1 cut(s) 460
PsuI RGATCY 1 cut(s) 100
RsaI GTAC 2 cut(s) 206, 683
RsaNI GTAC 2 cut(s) 205, 682
SaqAI TTAA 3 cut(s) 311, 648, 720
SatI GCNGC 1 cut(s) 426
Sau3AI GATC 1 cut(s) 100
Sau96I GGNCC 1 cut(s) 460
SchI GAGTC 2 cut(s) 374, 435
SfaNI GCATC 3 cut(s) 14, 19, 565
SfcI CTRYAG 1 cut(s) 501
SinI GGWCC 1 cut(s) 460
SmlI CTYRAG 1 cut(s) 719
SmoI CTYRAG 1 cut(s) 719
Sse9I AATT 5 cut(s) 50, 159, 238, 432, 642
SsiI CCGC 1 cut(s) 458
SspI AATATT 1 cut(s) 367
SspMI CTAG 1 cut(s) 305
StyI CCWWGG 1 cut(s) 408
TaaI ACNGT 1 cut(s) 715
TaiI ACGT 1 cut(s) 90
TaqI TCGA 3 cut(s) 45, 387, 640
TasI AATT 5 cut(s) 50, 159, 238, 432, 642
TfiI GAWTC 1 cut(s) 596
Tru1I TTAA 3 cut(s) 311, 648, 720
Tru9I TTAA 3 cut(s) 311, 648, 720
TseI GCWGC 1 cut(s) 425
TspDTI ATGAA 4 cut(s) 134, 236, 530, 609
Vha464I CTTAAG 1 cut(s) 719
VpaK11BI GGWCC 1 cut(s) 460
VspI ATTAAT 1 cut(s) 311
XceI RCATGY 1 cut(s) 660
XspI CTAG 1 cut(s) 305
ZraI GACGTC 1 cut(s) 88
Zsp2I ATGCAT 1 cut(s) 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.