Rroxscaffold_3G00235280

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000053
Physical Location & Seq
Forward (+)
22195830 .. 22204010
8181 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00235280.1

Sequence Viewer

Length: 1440 bp
ATGTCTGAAATTCCTGCTGTGACAGCAAACTATGAACCGGGCATTTGCGGAGATGGGTTCACCAACTGTGATTCTATATGCAACATAAGTCGTGCTCATATGCAACAGCAAATTGACCAACAGAAAGTATTAGTGAGGGAAGTTTTTACATCATCTTCATCTGGTTTATCTCATCAGCTGAAATTAATTGATGATATCCAGCGCTTAGGAGTGTCATACCATTTTAAAACAGAAATAGAAGAAGCACTGGAACGTATTCATGTTCAGTTGCATGATGGTGATCTTGGCCTATACAATGACTCTCTTCGTTTTCGTCTATTAAGACAACATGGATATAACGTTTCATCTGGAGATATATTCAACAAATTCAAAGATGCAATCGGTTACTTCAAGGTGAGCTTAATTCCCGATGTTCTGGGGATGCTAAGCTTGTATGAAGCAACGCATCTTAGGGTTCATGGAGAAGAAATACTAGAAGAGGCTCTTGTTTTCACCACTAATCATCTTGAGTTGGCGATAAGCCAGGTAAGCTATCCACTAACTGCACAAATATCTCAAGCTCTAGAACGACCTGTGTGGAGAGGTCTAGAGAGACTATCTGCCAGGAATTATATGCCAATCTACCAAGCCACTACTTCGCATAATGAAGCATTACTGAAACTTGCAAAGTTAGATTTCAATTTGGTTCAATCTTTGCACAAAGAGGAGCTTAGCGAGGTTACCAGGTGGTGGAAAGCACTAGATTTTGAAAGCAATATCCCTTTTGCAAGGCATAGAGTTGTGGAGTGTTTCTTTTGGATGGTGGGAATGTATTTTGAACCTCAATACTCGGTTGGGAGAAAGATCGGAACAAAACTGTGTTCTATGGCTACAATGTTGGATGATATCTACGATTCATTTGGTACATTTGAACAACTCAAGATATTTCGTGAAGCAATCGACAGGTGGGATGTCAACTTTTGCATGAATGGCCTACCAGGATACATGAAAGTATTTTATCATTCACTTCTGAACCTTATGAATGAAATTGAGGAAGAGCTGGAAAAGCAAGGACTATCATACCGAGTTGAATATGCAAAACAAGTTCGGCGAATGGCCGAACGAGGTGCAGATCTGATTTGCGCTTGTATCCGACTTCTTTCAAATGATTGTAGGCCGAGGAAGGAACCCTCTCAGCCGTTAGGTTCTGATGCCTCTGTTGCAAGGACTTCGTACGACCCGGAGTTGGCTCTTCTCAACCAAACTAAAGCAAATCCCCCTATGTTCGGAACGCTCACTTCTCTTCCTATCGAAGGGATGTCCACAAGTGAGCAAGCTACAGTTTGCAATCCATCATCAGCCGAGCTTGTGTCTTTTGACAATAAGAGAGACTTCTTGAAGCGGAAAGTTAATCGCGGCATGCAAGGCTTGGAAAATGATAGAGAAGCCATTGAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

479

Amino Acids

54.76

Weight (kDa)

5.56

Isoelectric Point (pI)

48.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 29 - 188 7.9e-49 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 245 - 361 4.8e-42 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 285 - 357 1.4e-12 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 729
AccII CGCG 1 cut(s) 1395
AciI CCGC 3 cut(s) 48, 1381, 1395
AclI AACGTT 1 cut(s) 339
AcoI YGGCCR 1 cut(s) 1095
AcsI RAATTY 2 cut(s) 9, 365
AfaI GTAC 2 cut(s) 904, 1214
AfeI AGCGCT 1 cut(s) 203
AfiI CCNNNNNNNGG 4 cut(s) 729, 1225, 1265, 1292
AjnI CCWGG 4 cut(s) 522, 602, 722, 976
AluBI AGCT 9 cut(s) 178, 399, 429, 531, 560, 709, 1039, 1316, 1345
AluI AGCT 9 cut(s) 178, 399, 429, 531, 560, 709, 1039, 1316, 1345
Alw21I GWGCWC 1 cut(s) 97
Alw26I GTCTC 2 cut(s) 586, 1362
Aor51HI AGCGCT 1 cut(s) 203
AoxI GGCC 4 cut(s) 286, 970, 1095, 1154
ApoI RAATTY 2 cut(s) 9, 365
AseI ATTAAT 1 cut(s) 185
Asp700I GAANNNNTTC 1 cut(s) 255
AspLEI GCGC 2 cut(s) 204, 1124
AsuC2I CCSGG 2 cut(s) 39, 1220
AsuHPI GGTGA 4 cut(s) 52, 290, 406, 484
Bbv12I GWGCWC 1 cut(s) 97
BccI CCATC 4 cut(s) 47, 269, 793, 1339
BceAI ACGGC 1 cut(s) 1162
BcgI CGANNNNNNTGC 4 cut(s) 1088, 1122, 1191, 1225
BciT130I CCWGG 4 cut(s) 524, 604, 724, 978
BciVI GTATCC 2 cut(s) 974, 1139
BcnI CCSGG 2 cut(s) 39, 1220
BcoDI GTCTC 2 cut(s) 586, 1362
BfaI CTAG 4 cut(s) 473, 563, 587, 740
BfmI CTRYAG 1 cut(s) 1317
BfoI RGCGCY 1 cut(s) 205
BfuI GTATCC 2 cut(s) 974, 1139
BglII AGATCT 1 cut(s) 1111
BisI GCNGC 1 cut(s) 1396
BlpI GCTNAGC 2 cut(s) 425, 710
BlsI GCNGC 1 cut(s) 1397
Bme1390I CCNGG 6 cut(s) 39, 524, 604, 724, 978, 1220
BmiI GGNNCC 1 cut(s) 1167
BmrFI CCNGG 6 cut(s) 39, 524, 604, 724, 978, 1220
BmsI GCATC 4 cut(s) 364, 411, 454, 1180
BpmI CTGGAG 1 cut(s) 369
Bpu10I CCTNAGC 1 cut(s) 205
Bpu1102I GCTNAGC 2 cut(s) 425, 710
BpuEI CTTGAG 3 cut(s) 527, 540, 902
BpuMI CCSGG 2 cut(s) 39, 1220
BsaBI GATNNNNATC 1 cut(s) 279
BsaJI CCNNGG 1 cut(s) 1157
Bsc4I CCNNNNNNNGG 4 cut(s) 729, 1225, 1265, 1292
Bse1I ACTGG 1 cut(s) 252
Bse8I GATNNNNATC 1 cut(s) 279
BseBI CCWGG 4 cut(s) 524, 604, 724, 978
BseDI CCNNGG 1 cut(s) 1157
BseGI GGATG 5 cut(s) 426, 804, 886, 955, 1302
BseJI GATNNNNATC 1 cut(s) 279
BseLI CCNNNNNNNGG 4 cut(s) 729, 1225, 1265, 1292
BseMII CTCAG 1 cut(s) 1187
BseNI ACTGG 1 cut(s) 252
BseRI GAGGAG 1 cut(s) 719
BsgI GTGCAG 2 cut(s) 528, 1128
Bsh1236I CGCG 1 cut(s) 1395
BshFI GGCC 4 cut(s) 288, 972, 1097, 1156
BsiHKAI GWGCWC 1 cut(s) 97
BsiSI CCGG 2 cut(s) 38, 1220
BsiWI CGTACG 1 cut(s) 1212
BslI CCNNNNNNNGG 4 cut(s) 729, 1225, 1265, 1292
BsmAI GTCTC 2 cut(s) 586, 1362
BsnI GGCC 4 cut(s) 288, 972, 1097, 1156
Bsp1286I GDGCHC 1 cut(s) 97
Bsp143I GATC 3 cut(s) 280, 843, 1111
Bsp1720I GCTNAGC 2 cut(s) 425, 710
BspACI CCGC 3 cut(s) 48, 1381, 1395
BspANI GGCC 4 cut(s) 288, 972, 1097, 1156
BspCNI CTCAG 1 cut(s) 1186
BspFNI CGCG 1 cut(s) 1395
BspLI GGNNCC 1 cut(s) 1167
BspQI GCTCTTC 2 cut(s) 1029, 1236
BsrI ACTGG 1 cut(s) 252
BssECI CCNNGG 1 cut(s) 1157
BssMI GATC 3 cut(s) 280, 843, 1111
Bst2UI CCWGG 4 cut(s) 524, 604, 724, 978
Bst4CI ACNGT 3 cut(s) 68, 858, 1321
Bst6I CTCTTC 5 cut(s) 309, 471, 1029, 1236, 1287
BstC8I GCNNGC 2 cut(s) 1314, 1400
BstDEI CTNAG 5 cut(s) 205, 425, 449, 710, 1173
BstEII GGTNACC 1 cut(s) 718
BstENI CCTNNNNNAGG 1 cut(s) 1290
BstF5I GGATG 5 cut(s) 426, 804, 886, 955, 1302
BstFNI CGCG 1 cut(s) 1395
BstH2I RGCGCY 1 cut(s) 205
BstHHI GCGC 2 cut(s) 204, 1124
BstKTI GATC 3 cut(s) 283, 846, 1114
BstMAI GTCTC 2 cut(s) 586, 1362
BstMBI GATC 3 cut(s) 280, 843, 1111
BstMWI GCNNNNNNNGC 6 cut(s) 23, 528, 969, 1045, 1199, 1404
BstNI CCWGG 4 cut(s) 524, 604, 724, 978
BstNSI RCATGY 1 cut(s) 1402
BstPI GGTNACC 1 cut(s) 718
BstSCI CCNGG 6 cut(s) 37, 522, 602, 722, 976, 1218
BstSFI CTRYAG 1 cut(s) 1317
BstUI CGCG 1 cut(s) 1395
BstX2I RGATCY 1 cut(s) 1111
BstYI RGATCY 1 cut(s) 1111
BsuI GTATCC 2 cut(s) 974, 1139
BsuRI GGCC 4 cut(s) 288, 972, 1097, 1156
BtsCI GGATG 5 cut(s) 426, 804, 886, 955, 1302
BtsIMutI CAGTG 1 cut(s) 245
Cac8I GCNNGC 2 cut(s) 1314, 1400
CfoI GCGC 2 cut(s) 204, 1124
CsiI ACCWGGT 1 cut(s) 722
Csp6I GTAC 2 cut(s) 903, 1213
CviAII CATG 7 cut(s) 260, 272, 329, 458, 964, 985, 1399
CviQI GTAC 2 cut(s) 903, 1213
DdeI CTNAG 5 cut(s) 205, 425, 449, 710, 1173
DpnI GATC 3 cut(s) 282, 845, 1113
DpnII GATC 3 cut(s) 280, 843, 1111
DraI TTTAAA 1 cut(s) 226
EaeI YGGCCR 1 cut(s) 1095
Eam1104I CTCTTC 5 cut(s) 309, 471, 1029, 1236, 1287
EarI CTCTTC 5 cut(s) 309, 471, 1029, 1236, 1287
Eco32I GATATC 2 cut(s) 196, 886
Eco47III AGCGCT 1 cut(s) 203
Eco91I GGTNACC 1 cut(s) 718
EcoNI CCTNNNNNAGG 1 cut(s) 1290
EcoO65I GGTNACC 1 cut(s) 718
EcoRII CCWGG 4 cut(s) 522, 602, 722, 976
EcoRV GATATC 2 cut(s) 196, 886
FaeI CATG 7 cut(s) 263, 275, 332, 461, 967, 988, 1402
FalI AAGNNNNNCTT 8 cut(s) 383, 415, 468, 500, 693, 725, 1355, 1387
FatI CATG 7 cut(s) 259, 271, 328, 457, 963, 984, 1398
FauNDI CATATG 1 cut(s) 99
Fnu4HI GCNGC 1 cut(s) 1396
FokI GGATG 5 cut(s) 433, 811, 893, 962, 1309
Fsp4HI GCNGC 1 cut(s) 1396
FspBI CTAG 4 cut(s) 473, 563, 587, 740
GlaI GCGC 2 cut(s) 203, 1123
GluI GCNGC 1 cut(s) 1396
GsuI CTGGAG 1 cut(s) 369
HaeII RGCGCY 1 cut(s) 205
HaeIII GGCC 4 cut(s) 288, 972, 1097, 1156
HapII CCGG 2 cut(s) 38, 1220
HhaI GCGC 2 cut(s) 204, 1124
Hin1II CATG 7 cut(s) 263, 275, 332, 461, 967, 988, 1402
Hin6I GCGC 2 cut(s) 202, 1122
HinP1I GCGC 2 cut(s) 202, 1122
HincII GTYRAC 1 cut(s) 955
HindII GTYRAC 1 cut(s) 955
HindIII AAGCTT 1 cut(s) 427
HinfI GANTC 3 cut(s) 71, 299, 893
HpaII CCGG 2 cut(s) 38, 1220
HphI GGTGA 4 cut(s) 52, 290, 406, 484
Hpy166II GTNNAC 3 cut(s) 60, 955, 1302
Hpy188I TCNGA 7 cut(s) 7, 848, 1011, 1116, 1133, 1189, 1268
Hpy188III TCNNGA 8 cut(s) 348, 407, 506, 563, 587, 919, 929, 1375
Hpy8I GTNNAC 3 cut(s) 60, 955, 1302
HpyAV CCTTC 2 cut(s) 1156, 1286
HpyCH4III ACNGT 3 cut(s) 68, 858, 1321
HpyCH4IV ACGT 2 cut(s) 253, 339
HpyF10VI GCNNNNNNNGC 6 cut(s) 23, 528, 969, 1045, 1199, 1404
HpyF3I CTNAG 5 cut(s) 205, 425, 449, 710, 1173
HpySE526I ACGT 2 cut(s) 253, 339
Hsp92II CATG 7 cut(s) 263, 275, 332, 461, 967, 988, 1402
HspAI GCGC 2 cut(s) 202, 1122
Kzo9I GATC 3 cut(s) 280, 843, 1111
LguI GCTCTTC 2 cut(s) 1029, 1236
LmnI GCTCC 1 cut(s) 706
LweI GCATC 4 cut(s) 364, 411, 454, 1180
MabI ACCWGGT 1 cut(s) 722
MaeI CTAG 4 cut(s) 473, 563, 587, 740
MaeII ACGT 2 cut(s) 253, 339
MaeIII GTNAC 3 cut(s) 19, 383, 718
MalI GATC 3 cut(s) 282, 845, 1113
MboI GATC 3 cut(s) 280, 843, 1111
MboII GAAGA 8 cut(s) 147, 251, 296, 476, 488, 1046, 1223, 1274
MflI RGATCY 1 cut(s) 1111
MhlI GDGCHC 1 cut(s) 97
MluCI AATT 9 cut(s) 9, 111, 182, 186, 365, 402, 607, 679, 1026
MlyI GAGTC 1 cut(s) 293
MmeI TCCRAC 2 cut(s) 858, 1156
MroXI GAANNNNTTC 1 cut(s) 255
MseI TTAA 5 cut(s) 185, 225, 320, 401, 1389
MslI CAYNNNNRTG 1 cut(s) 276
MspA1I CMGCKG 1 cut(s) 178
MspI CCGG 2 cut(s) 38, 1220
MspR9I CCNGG 6 cut(s) 39, 524, 604, 724, 978, 1220
MvaI CCWGG 4 cut(s) 524, 604, 724, 978
MvnI CGCG 1 cut(s) 1395
MwoI GCNNNNNNNGC 6 cut(s) 23, 528, 969, 1045, 1199, 1404
NciI CCSGG 2 cut(s) 39, 1220
NdeI CATATG 1 cut(s) 99
NdeII GATC 3 cut(s) 280, 843, 1111
NlaIII CATG 7 cut(s) 263, 275, 332, 461, 967, 988, 1402
NlaIV GGNNCC 1 cut(s) 1167
NmeAIII GCCGAG 2 cut(s) 1182, 1366
NmuCI GTSAC 1 cut(s) 19
NspI RCATGY 1 cut(s) 1402
PaeI GCATGC 1 cut(s) 1402
PciSI GCTCTTC 2 cut(s) 1029, 1236
PdmI GAANNNNTTC 1 cut(s) 255
PfeI GAWTC 2 cut(s) 71, 893
Pfl23II CGTACG 1 cut(s) 1212
PflMI CCANNNNNTGG 1 cut(s) 729
PkrI GCNGC 1 cut(s) 1397
PleI GAGTC 1 cut(s) 293
PpsI GAGTC 1 cut(s) 293
PshBI ATTAAT 1 cut(s) 185
Psp1406I AACGTT 1 cut(s) 339
Psp6I CCWGG 4 cut(s) 522, 602, 722, 976
PspEI GGTNACC 1 cut(s) 718
PspGI CCWGG 4 cut(s) 522, 602, 722, 976
PspLI CGTACG 1 cut(s) 1212
PspN4I GGNNCC 1 cut(s) 1167
PsuI RGATCY 1 cut(s) 1111
PvuII CAGCTG 1 cut(s) 178
RsaI GTAC 2 cut(s) 904, 1214
RsaNI GTAC 2 cut(s) 903, 1213
RseI CAYNNNNRTG 1 cut(s) 276
SapI GCTCTTC 2 cut(s) 1029, 1236
SaqAI TTAA 5 cut(s) 185, 225, 320, 401, 1389
SatI GCNGC 1 cut(s) 1396
Sau3AI GATC 3 cut(s) 280, 843, 1111
SchI GAGTC 1 cut(s) 293
ScrFI CCNGG 6 cut(s) 39, 524, 604, 724, 978, 1220
SduI GDGCHC 1 cut(s) 97
SexAI ACCWGGT 1 cut(s) 722
SfaNI GCATC 4 cut(s) 364, 411, 454, 1180
SfcI CTRYAG 1 cut(s) 1317
SmiMI CAYNNNNRTG 1 cut(s) 276
SmlI CTYRAG 3 cut(s) 506, 555, 917
SmoI CTYRAG 3 cut(s) 506, 555, 917
SphI GCATGC 1 cut(s) 1402
Sse9I AATT 9 cut(s) 9, 111, 182, 186, 365, 402, 607, 679, 1026
SsiI CCGC 3 cut(s) 48, 1381, 1395
SspMI CTAG 4 cut(s) 473, 563, 587, 740
StyD4I CCNGG 6 cut(s) 37, 522, 602, 722, 976, 1218
TaaI ACNGT 3 cut(s) 68, 858, 1321
TaiI ACGT 2 cut(s) 256, 342
TaqI TCGA 2 cut(s) 939, 1290
TasI AATT 9 cut(s) 9, 111, 182, 186, 365, 402, 607, 679, 1026
TauI GCSGC 1 cut(s) 1398
TfiI GAWTC 2 cut(s) 71, 893
Tru1I TTAA 5 cut(s) 185, 225, 320, 401, 1389
Tru9I TTAA 5 cut(s) 185, 225, 320, 401, 1389
TscAI CASTG 1 cut(s) 252
TseFI GTSAC 1 cut(s) 19
Tsp45I GTSAC 1 cut(s) 19
TspRI CASTG 1 cut(s) 252
Van91I CCANNNNNTGG 1 cut(s) 729
VspI ATTAAT 1 cut(s) 185
XagI CCTNNNNNAGG 1 cut(s) 1290
XapI RAATTY 2 cut(s) 9, 365
XbaI TCTAGA 2 cut(s) 562, 586
XceI RCATGY 1 cut(s) 1402
XmnI GAANNNNTTC 1 cut(s) 255
XspI CTAG 4 cut(s) 473, 563, 587, 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.