Prupe.4G194300_v2.0.a1

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000053
Physical Location & Seq
Forward (+)
11769339 .. 11772113
2775 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G194300.1

Sequence Viewer

Length: 1674 bp
ATGTCTATCCAAGTTTGTGCAGCAGCTCAATCACCAAATGCCAAGCCTGAAATTATTCGGCGGACAGCAAACTTCCCACCAAGCATTTGGGGTGATCGGTTTCTCAATTATGATTCCCAAGACATTATAACAAATGCCCGGAACCAACAAGAAGTTAACGAGCTGAAAGAAGTAGTGAGGAGGGAAGTATTAACAACTAGTGCAGGTGACTTTTCACATCAGCTCAAGTTGATTGATGCAATCCAGCGCCTTGGCGTGGCATACCATTTTGAAAGTGAAATTGAAGAAGCATTGGAGCGTAGGCATGCTGCATTTCGTGACCATGATGGAGATCTATACAACGTTGCTCTTGGTTTCCGGCTACTAAGACAACATGGATATAAGGTTTCATGTGATGTGTTCAACAAGTTCAAAGACGAAAATGGCAGCTTCAAGGAATGCTTAATTGCCGATGTTCCGGGGATGCTAAGCCTTTATGAAGCCGGGCATCTAGGCATACGGGGAGAAGAAATACTAGATGAAGCTCTTGCTTTCACCACCACTCACCTTGATTCTGCTGCAAAAGCTCATGTAAGCTACGAACATGCAGAACAAATCACTCAAGCCTTGGAGAGACCTTTGCGAAAAGATCTAGAAAGGGTATGTGCCAGGCGTTACATGTCAATCTACCAAGATGAAGCTTCACACAACGAAGCTTTGTTGAAACTTGCGAAGTTAGACTTCAATCTTGTTCAGTCTTTGCACAAAAAGGAGCTCAGTGAGATTACTAGGTGGTGGAAAGAAGTAGACTTTGAAAAGAAGCTGCCTTTCGCAAGAAATAGGATCGTGGAGTTGTACTTTTGGGTAGTCGGAGTATACTTTGAACCCCAAAACGTCGAAGCAAGAAAATTTCTTACAAAAGTGATTGCGCTGGTATCAGTGATGGATGATATCTACGACGCATATGCTACGTTTGAAGAACTTGAGATCTTTACTGGAGCAATTGAGAGGTGGGATATGAGTTGCATGGATGAACTTCCAGACTATATGCAAATATTTTATCGTACCCTTTTGACTGTTGTCGATGAAATTGAGGAAGAGATTGCAAAGGATGGAAGATCGTACCGAGTTTATTATGCAAAGGAATCTTTGAAAGCTTTAGCCCGAGCTTACTTTGAGGAGGCCAGATGGTTCAATGAAGGATACACCCCAACCATGGATGAGTATCTACCTGCTGCAATAGTTTCTAGTGGCTACCCCATGCTTTCAACTGTATCCTTACTTGGCATGGGAGATATTGTAACAAAGGAGACATTTGAGTGGCTGTTCAATGACCAAAAAATTGTTAGAGCTTCCGCGACCTTGTGCAGGTTCATGGATGATATTGTCTCAGGCCAGTTTGAGAAAGAGAGAGGCCATGTTGCCTGTAGTATTGATTGCTACGTGAGGCAATATGGGGTGTCGGAGCAAGAGGCACTTGATGCTTTGAACAAGCAAGTGGTGGATCTGTGGAAGGACATAAACGAGGAGTTTCTCAGACCAACTGCAGCGCCAATGGCTGTCCTAATGAGAGTTCTTAATTTAACAAAAGTGATAGATCTCCTTTACAAAGGTGACGATGGCTACACGCGTGTTGGTAAAGTGGTGAAGGATAAGATTGCTTCACATTTTATTGATCCAGTGCCAATTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

558

Amino Acids

64.14

Weight (kDa)

5.1

Isoelectric Point (pI)

44.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 128
AarI CACCTGC 1 cut(s) 194
Acc36I ACCTGC 3 cut(s) 194, 1219, 1338
AccI GTMKAC 2 cut(s) 786, 855
AccII CGCG 2 cut(s) 1337, 1609
AciI CCGC 2 cut(s) 61, 1335
AclI AACGTT 1 cut(s) 342
AclWI GGATC 3 cut(s) 830, 1491, 1649
AcsI RAATTY 1 cut(s) 887
AfaI GTAC 3 cut(s) 836, 1045, 1103
AfiI CCNNNNNNNGG 3 cut(s) 256, 1195, 1347
AflIII ACRYGT 2 cut(s) 657, 1607
AhlI ACTAGT 1 cut(s) 197
AjnI CCWGG 1 cut(s) 647
Alw21I GWGCWC 1 cut(s) 756
Alw26I GTCTC 3 cut(s) 607, 1283, 1372
AlwI GGATC 3 cut(s) 830, 1491, 1649
Ama87I CYCGRG 1 cut(s) 1143
AoxI GGCC 3 cut(s) 1161, 1372, 1393
ApeKI GCWGC 8 cut(s) 20, 23, 308, 426, 557, 802, 1214, 1526
ApoI RAATTY 1 cut(s) 887
Asp700I GAANNNNTTC 1 cut(s) 54
AspLEI GCGC 3 cut(s) 249, 910, 1531
AsuC2I CCSGG 3 cut(s) 139, 459, 484
AsuHPI GGTGA 7 cut(s) 24, 104, 218, 526, 536, 1604, 1636
AvaI CYCGRG 1 cut(s) 1143
BanII GRGCYC 1 cut(s) 756
Bbv12I GWGCWC 1 cut(s) 756
BbvI GCAGC 8 cut(s) 32, 35, 295, 438, 544, 789, 1201, 1538
BccI CCATC 5 cut(s) 320, 916, 1085, 1161, 1592
BcgI CGANNNNNNTGC 2 cut(s) 926, 960
BciT130I CCWGG 1 cut(s) 649
BciVI GTATCC 2 cut(s) 1175, 1264
BcnI CCSGG 3 cut(s) 139, 459, 484
BcoDI GTCTC 3 cut(s) 607, 1283, 1372
BcuI ACTAGT 1 cut(s) 197
BfaI CTAG 6 cut(s) 198, 491, 515, 632, 768, 1227
BfmI CTRYAG 2 cut(s) 1405, 1524
BfoI RGCGCY 2 cut(s) 250, 1532
BfuAI ACCTGC 3 cut(s) 194, 1219, 1338
BfuI GTATCC 2 cut(s) 1175, 1264
BglII AGATCT 4 cut(s) 331, 628, 966, 1576
BisI GCNGC 8 cut(s) 21, 24, 309, 427, 558, 803, 1215, 1527
BlpI GCTNAGC 1 cut(s) 467
BlsI GCNGC 8 cut(s) 22, 25, 310, 428, 559, 804, 1216, 1528
Bme1390I CCNGG 4 cut(s) 139, 459, 484, 649
BmeT110I CYCGRG 1 cut(s) 1143
BmiI GGNNCC 1 cut(s) 143
BmrFI CCNGG 4 cut(s) 139, 459, 484, 649
BmsI GCATC 4 cut(s) 226, 453, 496, 1450
BoxI GACNNNNGTC 1 cut(s) 1058
BpmI CTGGAG 1 cut(s) 996
Bpu1102I GCTNAGC 1 cut(s) 467
BpuEI CTTGAG 3 cut(s) 209, 585, 983
BpuMI CCSGG 3 cut(s) 139, 459, 484
BsaAI YACGTR 1 cut(s) 1423
BsaBI GATNNNNATC 2 cut(s) 330, 1203
BsaI GGTCTC 1 cut(s) 607
BsaJI CCNNGG 4 cut(s) 250, 458, 606, 1194
BsaXI ACNNNNNCTCC 2 cut(s) 1263, 1293
Bsc4I CCNNNNNNNGG 3 cut(s) 256, 1195, 1347
Bse1I ACTGG 3 cut(s) 979, 1375, 1658
Bse8I GATNNNNATC 2 cut(s) 330, 1203
BseBI CCWGG 1 cut(s) 649
BseDI CCNNGG 4 cut(s) 250, 458, 606, 1194
BseGI GGATG 6 cut(s) 468, 931, 1015, 1096, 1204, 1363
BseJI GATNNNNATC 2 cut(s) 330, 1203
BseLI CCNNNNNNNGG 3 cut(s) 256, 1195, 1347
BseMII CTCAG 3 cut(s) 769, 1383, 1528
BseNI ACTGG 3 cut(s) 979, 1375, 1658
BseRI GAGGAG 3 cut(s) 193, 1172, 1520
BseXI GCAGC 8 cut(s) 32, 35, 295, 438, 544, 789, 1201, 1538
BsgI GTGCAG 3 cut(s) 39, 222, 1366
Bsh1236I CGCG 2 cut(s) 1337, 1609
BshFI GGCC 3 cut(s) 1163, 1374, 1395
BsiHKAI GWGCWC 1 cut(s) 756
BsiHKCI CYCGRG 1 cut(s) 1143
BsiSI CCGG 4 cut(s) 139, 358, 458, 483
BslI CCNNNNNNNGG 3 cut(s) 256, 1195, 1347
BsmAI GTCTC 3 cut(s) 607, 1283, 1372
BsmI GAATGC 1 cut(s) 443
BsnI GGCC 3 cut(s) 1163, 1374, 1395
Bso31I GGTCTC 1 cut(s) 607
BsoBI CYCGRG 1 cut(s) 1143
Bsp1286I GDGCHC 1 cut(s) 756
Bsp143I GATC 9 cut(s) 94, 331, 628, 822, 966, 1097, 1483, 1576, 1654
Bsp1720I GCTNAGC 1 cut(s) 467
Bsp19I CCATGG 1 cut(s) 1194
BspACI CCGC 2 cut(s) 61, 1335
BspANI GGCC 3 cut(s) 1163, 1374, 1395
BspCNI CTCAG 3 cut(s) 768, 1382, 1527
BspFNI CGCG 2 cut(s) 1337, 1609
BspLI GGNNCC 1 cut(s) 143
BspMAI CTGCAG 1 cut(s) 1528
BspMI ACCTGC 3 cut(s) 194, 1219, 1338
BspPI GGATC 3 cut(s) 830, 1491, 1649
BspTNI GGTCTC 1 cut(s) 607
BsrI ACTGG 3 cut(s) 979, 1375, 1658
BssECI CCNNGG 4 cut(s) 250, 458, 606, 1194
BssMI GATC 9 cut(s) 94, 331, 628, 822, 966, 1097, 1483, 1576, 1654
BssNAI GTATAC 1 cut(s) 856
BssT1I CCWWGG 3 cut(s) 250, 606, 1194
Bst1107I GTATAC 1 cut(s) 856
Bst2UI CCWGG 1 cut(s) 649
Bst4CI ACNGT 2 cut(s) 1057, 1252
Bst6I CTCTTC 1 cut(s) 1071
BstAPI GCANNNNNTGC 1 cut(s) 1460
BstBAI YACGTR 1 cut(s) 1423
BstC8I GCNNGC 1 cut(s) 306
BstDEI CTNAG 5 cut(s) 365, 467, 755, 1369, 1514
BstDSI CCRYGG 1 cut(s) 1194
BstENI CCTNNNNNAGG 1 cut(s) 1345
BstF5I GGATG 6 cut(s) 468, 931, 1015, 1096, 1204, 1363
BstFNI CGCG 2 cut(s) 1337, 1609
BstH2I RGCGCY 2 cut(s) 250, 1532
BstHHI GCGC 3 cut(s) 249, 910, 1531
BstKTI GATC 9 cut(s) 97, 334, 631, 825, 969, 1100, 1486, 1579, 1657
BstMAI GTCTC 3 cut(s) 607, 1283, 1372
BstMBI GATC 9 cut(s) 94, 331, 628, 822, 966, 1097, 1483, 1576, 1654
BstMWI GCNNNNNNNGC 3 cut(s) 563, 1460, 1535
BstNI CCWGG 1 cut(s) 649
BstNSI RCATGY 3 cut(s) 308, 587, 661
BstPAI GACNNNNGTC 1 cut(s) 1058
BstSCI CCNGG 4 cut(s) 137, 457, 482, 647
BstSFI CTRYAG 2 cut(s) 1405, 1524
BstUI CGCG 2 cut(s) 1337, 1609
BstV1I GCAGC 8 cut(s) 32, 35, 295, 438, 544, 789, 1201, 1538
BstX2I RGATCY 5 cut(s) 331, 628, 966, 1483, 1576
BstXI CCANNNNNNTGG 2 cut(s) 87, 251
BstYI RGATCY 5 cut(s) 331, 628, 966, 1483, 1576
BstZ17I GTATAC 1 cut(s) 856
BsuI GTATCC 2 cut(s) 1175, 1264
BsuRI GGCC 3 cut(s) 1163, 1374, 1395
BtgI CCRYGG 1 cut(s) 1194
BtsCI GGATG 6 cut(s) 468, 931, 1015, 1096, 1204, 1363
BtsIMutI CAGTG 3 cut(s) 763, 924, 1665
BveI ACCTGC 3 cut(s) 194, 1219, 1338
Cac8I GCNNGC 1 cut(s) 306
CfoI GCGC 3 cut(s) 249, 910, 1531
CseI GACGC 1 cut(s) 947
Csp6I GTAC 3 cut(s) 835, 1044, 1102
CspCI CAANNNNNGTGG 2 cut(s) 529, 564
CviQI GTAC 3 cut(s) 835, 1044, 1102
DdeI CTNAG 5 cut(s) 365, 467, 755, 1369, 1514
DpnI GATC 9 cut(s) 96, 333, 630, 824, 968, 1099, 1485, 1578, 1656
DpnII GATC 9 cut(s) 94, 331, 628, 822, 966, 1097, 1483, 1576, 1654
Eam1104I CTCTTC 1 cut(s) 1071
EarI CTCTTC 1 cut(s) 1071
EciI GGCGGA 1 cut(s) 76
Ecl136II GAGCTC 1 cut(s) 754
Eco130I CCWWGG 3 cut(s) 250, 606, 1194
Eco24I GRGCYC 1 cut(s) 756
Eco31I GGTCTC 1 cut(s) 607
Eco32I GATATC 1 cut(s) 931
Eco53kI GAGCTC 1 cut(s) 754
Eco88I CYCGRG 1 cut(s) 1143
EcoICRI GAGCTC 1 cut(s) 754
EcoNI CCTNNNNNAGG 1 cut(s) 1345
EcoRII CCWGG 1 cut(s) 647
EcoRV GATATC 1 cut(s) 931
EcoT14I CCWWGG 3 cut(s) 250, 606, 1194
EcoT38I GRGCYC 1 cut(s) 756
ErhI CCWWGG 3 cut(s) 250, 606, 1194
FalI AAGNNNNNCTT 6 cut(s) 425, 457, 704, 736, 1440, 1472
FauNDI CATATG 1 cut(s) 943
FblI GTMKAC 2 cut(s) 786, 855
Fnu4HI GCNGC 8 cut(s) 21, 24, 309, 427, 558, 803, 1215, 1527
FokI GGATG 6 cut(s) 475, 938, 1022, 1103, 1211, 1370
FriOI GRGCYC 1 cut(s) 756
Fsp4HI GCNGC 8 cut(s) 21, 24, 309, 427, 558, 803, 1215, 1527
FspBI CTAG 6 cut(s) 198, 491, 515, 632, 768, 1227
GlaI GCGC 3 cut(s) 248, 909, 1530
GluI GCNGC 8 cut(s) 21, 24, 309, 427, 558, 803, 1215, 1527
GsuI CTGGAG 1 cut(s) 996
HaeII RGCGCY 2 cut(s) 250, 1532
HaeIII GGCC 3 cut(s) 1163, 1374, 1395
HapII CCGG 4 cut(s) 139, 358, 458, 483
HgaI GACGC 1 cut(s) 947
HhaI GCGC 3 cut(s) 249, 910, 1531
Hin6I GCGC 3 cut(s) 247, 908, 1529
HinP1I GCGC 3 cut(s) 247, 908, 1529
HincII GTYRAC 1 cut(s) 157
HindII GTYRAC 1 cut(s) 157
HindIII AAGCTT 3 cut(s) 678, 693, 1134
HinfI GANTC 3 cut(s) 113, 551, 1124
HpaI GTTAAC 1 cut(s) 157
HpaII CCGG 4 cut(s) 139, 358, 458, 483
HphI GGTGA 7 cut(s) 24, 104, 218, 526, 536, 1604, 1636
Hpy166II GTNNAC 3 cut(s) 157, 787, 856
Hpy188I TCNGA 3 cut(s) 851, 1444, 1517
Hpy188III TCNNGA 3 cut(s) 317, 632, 1019
Hpy8I GTNNAC 3 cut(s) 157, 787, 856
Hpy99I CGWCG 2 cut(s) 878, 941
HpyAV CCTTC 3 cut(s) 1172, 1486, 1621
HpyCH4III ACNGT 2 cut(s) 1057, 1252
HpyCH4IV ACGT 4 cut(s) 342, 873, 950, 1422
HpyF10VI GCNNNNNNNGC 3 cut(s) 563, 1460, 1535
HpyF3I CTNAG 5 cut(s) 365, 467, 755, 1369, 1514
HpySE526I ACGT 4 cut(s) 342, 873, 950, 1422
HspAI GCGC 3 cut(s) 247, 908, 1529
KspAI GTTAAC 1 cut(s) 157
Kzo9I GATC 9 cut(s) 94, 331, 628, 822, 966, 1097, 1483, 1576, 1654
LmnI GCTCC 4 cut(s) 295, 751, 977, 1444
Lsp1109I GCAGC 8 cut(s) 32, 35, 295, 438, 544, 789, 1201, 1538
LweI GCATC 4 cut(s) 226, 453, 496, 1450
MaeI CTAG 6 cut(s) 198, 491, 515, 632, 768, 1227
MaeII ACGT 4 cut(s) 342, 873, 950, 1422
MaeIII GTNAC 5 cut(s) 206, 317, 653, 1279, 1592
MalI GATC 9 cut(s) 96, 333, 630, 824, 968, 1099, 1485, 1578, 1656
MboI GATC 9 cut(s) 94, 331, 628, 822, 966, 1097, 1483, 1576, 1654
MboII GAAGA 5 cut(s) 296, 518, 968, 1088, 1107
MfeI CAATTG 1 cut(s) 981
MflI RGATCY 5 cut(s) 331, 628, 966, 1483, 1576
MhlI GDGCHC 1 cut(s) 756
MluI ACGCGT 1 cut(s) 1607
MmeI TCCRAC 2 cut(s) 829, 1422
MroXI GAANNNNTTC 1 cut(s) 54
MseI TTAA 5 cut(s) 156, 191, 443, 1557, 1562
MslI CAYNNNNRTG 1 cut(s) 1297
MspI CCGG 4 cut(s) 139, 358, 458, 483
MspR9I CCNGG 4 cut(s) 139, 459, 484, 649
MunI CAATTG 1 cut(s) 981
Mva1269I GAATGC 1 cut(s) 443
MvaI CCWGG 1 cut(s) 649
MvnI CGCG 2 cut(s) 1337, 1609
MwoI GCNNNNNNNGC 3 cut(s) 563, 1460, 1535
NciI CCSGG 3 cut(s) 139, 459, 484
NcoI CCATGG 1 cut(s) 1194
NdeI CATATG 1 cut(s) 943
NdeII GATC 9 cut(s) 94, 331, 628, 822, 966, 1097, 1483, 1576, 1654
NlaIV GGNNCC 1 cut(s) 143
NmuCI GTSAC 3 cut(s) 206, 317, 1592
NspI RCATGY 3 cut(s) 308, 587, 661
PaeI GCATGC 1 cut(s) 308
PaqCI CACCTGC 1 cut(s) 194
PciI ACATGT 1 cut(s) 657
PctI GAATGC 1 cut(s) 443
PdmI GAANNNNTTC 1 cut(s) 54
PfeI GAWTC 3 cut(s) 113, 551, 1124
PkrI GCNGC 8 cut(s) 22, 25, 310, 428, 559, 804, 1216, 1528
Ppu21I YACGTR 1 cut(s) 1423
PscI ACATGT 1 cut(s) 657
PshAI GACNNNNGTC 1 cut(s) 1058
PsiI TTATAA 1 cut(s) 128
Psp124BI GAGCTC 1 cut(s) 756
Psp1406I AACGTT 1 cut(s) 342
Psp6I CCWGG 1 cut(s) 647
PspGI CCWGG 1 cut(s) 647
PspN4I GGNNCC 1 cut(s) 143
PstI CTGCAG 1 cut(s) 1528
PsuI RGATCY 5 cut(s) 331, 628, 966, 1483, 1576
RsaI GTAC 3 cut(s) 836, 1045, 1103
RsaNI GTAC 3 cut(s) 835, 1044, 1102
RseI CAYNNNNRTG 1 cut(s) 1297
SacI GAGCTC 1 cut(s) 756
SaqAI TTAA 5 cut(s) 156, 191, 443, 1557, 1562
SatI GCNGC 8 cut(s) 21, 24, 309, 427, 558, 803, 1215, 1527
Sau3AI GATC 9 cut(s) 94, 331, 628, 822, 966, 1097, 1483, 1576, 1654
ScrFI CCNGG 4 cut(s) 139, 459, 484, 649
SduI GDGCHC 1 cut(s) 756
SfaNI GCATC 4 cut(s) 226, 453, 496, 1450
SfcI CTRYAG 2 cut(s) 1405, 1524
SmiMI CAYNNNNRTG 1 cut(s) 1297
SmlI CTYRAG 3 cut(s) 224, 600, 962
SmoI CTYRAG 3 cut(s) 224, 600, 962
SpeI ACTAGT 1 cut(s) 197
SphI GCATGC 1 cut(s) 308
SsiI CCGC 2 cut(s) 61, 1335
SspI AATATT 1 cut(s) 1035
SspMI CTAG 6 cut(s) 198, 491, 515, 632, 768, 1227
SstI GAGCTC 1 cut(s) 756
StyD4I CCNGG 4 cut(s) 137, 457, 482, 647
StyI CCWWGG 3 cut(s) 250, 606, 1194
TaaI ACNGT 2 cut(s) 1057, 1252
TaiI ACGT 4 cut(s) 345, 876, 953, 1425
TaqI TCGA 2 cut(s) 876, 1062
TatI WGTACW 1 cut(s) 834
TfiI GAWTC 3 cut(s) 113, 551, 1124
Tru1I TTAA 5 cut(s) 156, 191, 443, 1557, 1562
Tru9I TTAA 5 cut(s) 156, 191, 443, 1557, 1562
TscAI CASTG 3 cut(s) 763, 924, 1665
TseFI GTSAC 3 cut(s) 206, 317, 1592
TseI GCWGC 8 cut(s) 20, 23, 308, 426, 557, 802, 1214, 1526
Tsp45I GTSAC 3 cut(s) 206, 317, 1592
TspDTI ATGAA 8 cut(s) 378, 492, 534, 690, 1026, 1080, 1191, 1342
TspRI CASTG 3 cut(s) 763, 924, 1665
XagI CCTNNNNNAGG 1 cut(s) 1345
XapI RAATTY 1 cut(s) 887
XbaI TCTAGA 1 cut(s) 631
XceI RCATGY 3 cut(s) 308, 587, 661
XcmI CCANNNNNNNNNTGG 1 cut(s) 84
XmiI GTMKAC 2 cut(s) 786, 855
XmnI GAANNNNTTC 1 cut(s) 54
XspI CTAG 6 cut(s) 198, 491, 515, 632, 768, 1227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.