Rw3G017500

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000053
Physical Location & Seq
Forward (+)
19034779 .. 19037186
2408 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G017500.1

Sequence Viewer

Length: 1653 bp
ATGTCTATCCAAACTATTCCAGCAGCTGAATCCCAGATCAATGCTAAGCCAGAAGTTGTTCGGCGCACAGCAAACTATGAACCTAGCATTTGGGGAGATCGGTTTGCTAACTATGCTGAAGACATTGTAACTCAAGCTCATATGCAAAAACAAGTTGAGGAGCTGAAACAAGTAGTGAGGAGGGAAGTATTCACTGATGCTGCTGATGATTTTTCACATCAACTGAAGCTAATTGATGCAATCCAGCGCCTCGGTGTGGCTTACCATTTTGAAACTGAAATAGAACAAGCCCTGGAACGTATACATGCTACCTACCAAGATATTAACGATGATGATGGTGATCTGTTCAATGTTGCTCTTCGTTTTCGACTACTCAGAAGACATGGATATAATGTTTCCTGCGATGTATTCAACAAGTTCAAAGATGCTAATGGTGCCTTCAAGGAAAGCTTAGTTACTGATGTTTCTGGTATGCTAAGCTTCTATGAGGCAGCCCATCTAATGGTGCATGGAGAAAAATTACTAGAAGAGGCTCTCGTTTTTACCACCACTCATCTCCAGACTGCAACAACTTCAACCGGTGCAAGCTCTCTGCTGAAAGCACAAATAACTGAAGCCCTAGAGAGACCTCTATTAAAAACTGTAGAGAGGTTAGGTGCTCGGTGTTACATGTCAATATACCAAGATGAAGCTTCACACAATGAAGATTTACTGAAACTTGCCAAGTTAGATTTCATTGTTGTTCAGTGTTTACACAAAAAGGAACTCAGTGACATTATAAGATGGTATAAGGGACTAGACTTTGCAAGGAGGTTGCCTTTTGTTCGAGATAGGCTCGTGGAGATGTTCTTTTGGATAACGGGAGTATATTTTGAACCCCAATACGTGTTCGGGAGAAAAATTCTGACGAAGCTGCTTGAGTTAGTAACAGTAATGGATGATATATATGATGCATTCGGTACATTCGAAGAACTCGTGATATTAACTGAAGCAATTGAGAGATGGGATGCTAGTTGCATGGATCAACTGCCAGACTATATGCAACCATTTTATCAAACACTTCTGGATGTTGTCAATGAAGTTGGGGAGGAACTGATAAAGCACGGAAGATCATACCAACTTTACTATGCAAAAGAAATTACGAAGAACCAAGCCCGGCTCTACTTTGCTGAGGCCAGATGGTTCCACGAAGGATGCACCCCAAGAATGGATGAATATATGCGTGTTGCGGCATCTTCTTTCGTAGACATGGGAGACATTATAACAAAAGTAACATTTGAGTGGTTGACCAATGATCCTAAAATTCTTCGAGCTACGAATATCATGTCTAGGCTTATGGATGACATTGCTGGGCACAAGTTTGAGAAAGAGAGAGGGCATGTTGCTTCTAGCATCGATTGCTACATGAATGAATATGGGGTCTCAGAGCAAGAGACAATTGATGTCTTTAACAAACGAATTGTGGTTTCGTGGAAGGATATAAATAAAGAGTTTATGAGACCCACTGCTGCGCCAATGCCTGTGCTTAATCGTGTTCTTAACGCAGCTCGAGTGGTTGATCTCCTTTACAAAAGGGGAGATGGCTTCACGCATGTTGGAAAACAGATGGAAGACAATATTGCTTCACTGTTTATTGATCCAGTGCCACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

550

Amino Acids

63.56

Weight (kDa)

5.25

Isoelectric Point (pI)

45.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 30 - 207 4.8e-58 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 264 - 493 6.7e-85 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 304 - 493 3.5e-47 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 779, 1262
AccB1I GGYRCC 1 cut(s) 434
AccB7I CCANNNNNTGG 1 cut(s) 502
AccI GTMKAC 2 cut(s) 301, 1245
AciI CCGC 1 cut(s) 1229
AclWI GGATC 3 cut(s) 1029, 1289, 1631
AcsI RAATTY 2 cut(s) 900, 1302
AcuI CTGAAG 4 cut(s) 138, 245, 633, 1008
AfaI GTAC 1 cut(s) 961
AfiI CCNNNNNNNGG 3 cut(s) 256, 502, 1207
AflIII ACRYGT 2 cut(s) 669, 885
AgeI ACCGGT 1 cut(s) 578
AgsI TTSAA 7 cut(s) 272, 349, 412, 421, 442, 576, 875
AjnI CCWGG 1 cut(s) 291
AjuI GAANNNNNNNTTGG 2 cut(s) 716, 748
Alw21I GWGCWC 1 cut(s) 661
Alw26I GTCTC 5 cut(s) 619, 1248, 1426, 1427, 1492
AlwI GGATC 3 cut(s) 1029, 1289, 1631
AlwNI CAGNNNCTG 1 cut(s) 26
Ama87I CYCGRG 1 cut(s) 1548
AoxI GGCC 1 cut(s) 1173
ApeKI GCWGC 6 cut(s) 23, 200, 491, 913, 1508, 1544
ApoI RAATTY 2 cut(s) 900, 1302
ArsI GACNNNNNNTTYG 2 cut(s) 1310, 1342
AsiGI ACCGGT 1 cut(s) 578
Asp700I GAANNNNTTC 1 cut(s) 57
AspLEI GCGC 3 cut(s) 66, 249, 1513
AsuC2I CCSGG 1 cut(s) 1156
AsuHPI GGTGA 1 cut(s) 350
AsuII TTCGAA 1 cut(s) 966
AvaI CYCGRG 1 cut(s) 1548
BaeGI GKGCMC 1 cut(s) 1356
BanI GGYRCC 1 cut(s) 434
BauI CACGAG 2 cut(s) 836, 974
BbsI GAAGAC 3 cut(s) 126, 385, 1617
Bbv12I GWGCWC 1 cut(s) 661
BbvCI CCTCAGC 1 cut(s) 1170
BbvI GCAGC 6 cut(s) 35, 187, 503, 900, 1495, 1556
BccI CCATC 7 cut(s) 329, 504, 777, 996, 1173, 1574, 1600
BciT130I CCWGG 1 cut(s) 293
BcnI CCSGG 1 cut(s) 1156
BcoDI GTCTC 5 cut(s) 619, 1248, 1426, 1427, 1492
BfaI CTAG 7 cut(s) 84, 524, 620, 797, 1011, 1329, 1389
BfmI CTRYAG 1 cut(s) 642
BfoI RGCGCY 1 cut(s) 250
BisI GCNGC 7 cut(s) 24, 201, 492, 914, 1230, 1509, 1545
BlpI GCTNAGC 2 cut(s) 45, 476
BlsI GCNGC 7 cut(s) 25, 202, 493, 915, 1231, 1510, 1546
Bme1390I CCNGG 2 cut(s) 293, 1156
BmeT110I CYCGRG 1 cut(s) 1548
BmiI GGNNCC 2 cut(s) 436, 1184
BmrFI CCNGG 2 cut(s) 293, 1156
BmsI GCATC 8 cut(s) 187, 226, 415, 940, 997, 1184, 1241, 1401
BpiI GAAGAC 3 cut(s) 126, 385, 1617
BplI GAGNNNNNCTC 2 cut(s) 819, 851
BpmI CTGGAG 1 cut(s) 542
Bpu10I CCTNAGC 1 cut(s) 1170
Bpu1102I GCTNAGC 2 cut(s) 45, 476
Bpu14I TTCGAA 1 cut(s) 966
BpuEI CTTGAG 2 cut(s) 117, 938
BpuMI CCSGG 1 cut(s) 1156
Bsa29I ATCGAT 1 cut(s) 1395
BsaAI YACGTR 1 cut(s) 886
BsaBI GATNNNNATC 1 cut(s) 339
BsaI GGTCTC 3 cut(s) 619, 1426, 1492
BsaJI CCNNGG 2 cut(s) 250, 291
BsaWI WCCGGW 1 cut(s) 578
Bsc4I CCNNNNNNNGG 3 cut(s) 256, 502, 1207
Bse118I RCCGGY 1 cut(s) 578
Bse1I ACTGG 1 cut(s) 1640
Bse3DI GCAATG 1 cut(s) 1344
Bse8I GATNNNNATC 1 cut(s) 339
BseBI CCWGG 1 cut(s) 293
BseCI ATCGAT 1 cut(s) 1395
BseDI CCNNGG 2 cut(s) 250, 291
BseGI GGATG 6 cut(s) 943, 1012, 1072, 1199, 1216, 1345
BseJI GATNNNNATC 1 cut(s) 339
BseLI CCNNNNNNNGG 3 cut(s) 256, 502, 1207
BseMI GCAATG 1 cut(s) 1344
BseMII CTCAG 4 cut(s) 388, 781, 1161, 1437
BseNI ACTGG 1 cut(s) 1640
BseRI GAGGAG 2 cut(s) 173, 193
BseSI GKGCMC 1 cut(s) 1356
BseXI GCAGC 6 cut(s) 35, 187, 503, 900, 1495, 1556
BseYI CCCAGC 1 cut(s) 1349
BshFI GGCC 1 cut(s) 1175
BshNI GGYRCC 1 cut(s) 434
BshTI ACCGGT 1 cut(s) 578
BshVI ATCGAT 1 cut(s) 1395
BsiHKAI GWGCWC 1 cut(s) 661
BsiHKCI CYCGRG 1 cut(s) 1548
BsiSI CCGG 2 cut(s) 579, 1156
BslFI GGGAC 1 cut(s) 807
BslI CCNNNNNNNGG 3 cut(s) 256, 502, 1207
BsmAI GTCTC 5 cut(s) 619, 1248, 1426, 1427, 1492
BsmFI GGGAC 1 cut(s) 807
BsmI GAATGC 1 cut(s) 953
BsnI GGCC 1 cut(s) 1175
Bso31I GGTCTC 3 cut(s) 619, 1426, 1492
BsoBI CYCGRG 1 cut(s) 1548
Bsp119I TTCGAA 1 cut(s) 966
Bsp1286I GDGCHC 2 cut(s) 661, 1356
Bsp143I GATC 8 cut(s) 36, 97, 340, 1021, 1109, 1294, 1558, 1636
Bsp1720I GCTNAGC 2 cut(s) 45, 476
BspACI CCGC 1 cut(s) 1229
BspANI GGCC 1 cut(s) 1175
BspCNI CTCAG 4 cut(s) 387, 780, 1162, 1436
BspDI ATCGAT 1 cut(s) 1395
BspLI GGNNCC 2 cut(s) 436, 1184
BspPI GGATC 3 cut(s) 1029, 1289, 1631
BspQI GCTCTTC 1 cut(s) 363
BspT104I TTCGAA 1 cut(s) 966
BspT107I GGYRCC 1 cut(s) 434
BspTNI GGTCTC 3 cut(s) 619, 1426, 1492
BsrDI GCAATG 1 cut(s) 1344
BsrFI RCCGGY 1 cut(s) 578
BsrI ACTGG 1 cut(s) 1640
BssAI RCCGGY 1 cut(s) 578
BssECI CCNNGG 2 cut(s) 250, 291
BssMI GATC 8 cut(s) 36, 97, 340, 1021, 1109, 1294, 1558, 1636
BssNAI GTATAC 1 cut(s) 302
BssSI CACGAG 2 cut(s) 836, 974
Bst1107I GTATAC 1 cut(s) 302
Bst2BI CACGAG 2 cut(s) 836, 974
Bst2UI CCWGG 1 cut(s) 293
Bst4CI ACNGT 3 cut(s) 643, 931, 1629
Bst6I CTCTTC 2 cut(s) 363, 522
BstAPI GCANNNNNTGC 1 cut(s) 1398
BstBAI YACGTR 1 cut(s) 886
BstBI TTCGAA 1 cut(s) 966
BstC8I GCNNGC 1 cut(s) 586
BstDEI CTNAG 7 cut(s) 45, 374, 451, 476, 767, 1170, 1423
BstF5I GGATG 6 cut(s) 943, 1012, 1072, 1199, 1216, 1345
BstH2I RGCGCY 1 cut(s) 250
BstHHI GCGC 3 cut(s) 66, 249, 1513
BstKTI GATC 8 cut(s) 39, 100, 343, 1024, 1112, 1297, 1561, 1639
BstMAI GTCTC 5 cut(s) 619, 1248, 1426, 1427, 1492
BstMBI GATC 8 cut(s) 36, 97, 340, 1021, 1109, 1294, 1558, 1636
BstMWI GCNNNNNNNGC 3 cut(s) 113, 434, 1398
BstNI CCWGG 1 cut(s) 293
BstNSI RCATGY 4 cut(s) 308, 673, 1382, 1595
BstSCI CCNGG 2 cut(s) 291, 1154
BstSFI CTRYAG 1 cut(s) 642
BstSLI GKGCMC 1 cut(s) 1356
BstV1I GCAGC 6 cut(s) 35, 187, 503, 900, 1495, 1556
BstV2I GAAGAC 3 cut(s) 126, 385, 1617
BstZ17I GTATAC 1 cut(s) 302
Bsu15I ATCGAT 1 cut(s) 1395
BsuRI GGCC 1 cut(s) 1175
BsuTUI ATCGAT 1 cut(s) 1395
BtgZI GCGATG 1 cut(s) 417
BtsCI GGATG 6 cut(s) 943, 1012, 1072, 1199, 1216, 1345
BtsI GCAGTG 1 cut(s) 1503
BtsIMutI CAGTG 6 cut(s) 192, 752, 775, 1503, 1625, 1647
Cac8I GCNNGC 1 cut(s) 586
CaiI CAGNNNCTG 1 cut(s) 26
CfoI GCGC 3 cut(s) 66, 249, 1513
Cfr10I RCCGGY 1 cut(s) 578
ClaI ATCGAT 1 cut(s) 1395
Csp6I GTAC 1 cut(s) 960
CspAI ACCGGT 1 cut(s) 578
CviQI GTAC 1 cut(s) 960
DdeI CTNAG 7 cut(s) 45, 374, 451, 476, 767, 1170, 1423
DpnI GATC 8 cut(s) 38, 99, 342, 1023, 1111, 1296, 1560, 1638
DpnII GATC 8 cut(s) 36, 97, 340, 1021, 1109, 1294, 1558, 1636
Eam1104I CTCTTC 2 cut(s) 363, 522
EarI CTCTTC 2 cut(s) 363, 522
Eco31I GGTCTC 3 cut(s) 619, 1426, 1492
Eco57I CTGAAG 4 cut(s) 138, 245, 633, 1008
Eco88I CYCGRG 1 cut(s) 1548
EcoRII CCWGG 1 cut(s) 291
EcoT22I ATGCAT 1 cut(s) 955
FalI AAGNNNNNCTT 2 cut(s) 434, 466
FaqI GGGAC 1 cut(s) 807
FauNDI CATATG 1 cut(s) 141
FblI GTMKAC 2 cut(s) 301, 1245
Fnu4HI GCNGC 7 cut(s) 24, 201, 492, 914, 1230, 1509, 1545
FokI GGATG 6 cut(s) 950, 1019, 1079, 1206, 1223, 1352
Fsp4HI GCNGC 7 cut(s) 24, 201, 492, 914, 1230, 1509, 1545
FspBI CTAG 7 cut(s) 84, 524, 620, 797, 1011, 1329, 1389
GlaI GCGC 3 cut(s) 65, 248, 1512
GluI GCNGC 7 cut(s) 24, 201, 492, 914, 1230, 1509, 1545
GsaI CCCAGC 1 cut(s) 1353
GsuI CTGGAG 1 cut(s) 542
HaeII RGCGCY 1 cut(s) 250
HaeIII GGCC 1 cut(s) 1175
HapII CCGG 2 cut(s) 579, 1156
HhaI GCGC 3 cut(s) 66, 249, 1513
Hin6I GCGC 3 cut(s) 64, 247, 1511
HinP1I GCGC 3 cut(s) 64, 247, 1511
HincII GTYRAC 1 cut(s) 1287
HindII GTYRAC 1 cut(s) 1287
HindIII AAGCTT 3 cut(s) 448, 478, 690
HinfI GANTC 1 cut(s) 29
HpaII CCGG 2 cut(s) 579, 1156
HphI GGTGA 1 cut(s) 350
Hpy166II GTNNAC 4 cut(s) 302, 752, 1246, 1287
Hpy188I TCNGA 4 cut(s) 377, 906, 1426, 1652
Hpy188III TCNNGA 5 cut(s) 559, 827, 892, 976, 1064
Hpy8I GTNNAC 4 cut(s) 302, 752, 1246, 1287
HpyAV CCTTC 3 cut(s) 448, 1184, 1468
HpyCH4III ACNGT 3 cut(s) 643, 931, 1629
HpyCH4IV ACGT 2 cut(s) 298, 885
HpyF10VI GCNNNNNNNGC 3 cut(s) 113, 434, 1398
HpyF3I CTNAG 7 cut(s) 45, 374, 451, 476, 767, 1170, 1423
HpySE526I ACGT 2 cut(s) 298, 885
HspAI GCGC 3 cut(s) 64, 247, 1511
Kzo9I GATC 8 cut(s) 36, 97, 340, 1021, 1109, 1294, 1558, 1636
LguI GCTCTTC 1 cut(s) 363
LmnI GCTCC 1 cut(s) 160
Lsp1109I GCAGC 6 cut(s) 35, 187, 503, 900, 1495, 1556
LweI GCATC 8 cut(s) 187, 226, 415, 940, 997, 1184, 1241, 1401
MaeI CTAG 7 cut(s) 84, 524, 620, 797, 1011, 1329, 1389
MaeII ACGT 2 cut(s) 298, 885
MaeIII GTNAC 6 cut(s) 127, 454, 665, 770, 925, 1270
MalI GATC 8 cut(s) 38, 99, 342, 1023, 1111, 1296, 1560, 1638
MboI GATC 8 cut(s) 36, 97, 340, 1021, 1109, 1294, 1558, 1636
MfeI CAATTG 2 cut(s) 993, 1437
MhlI GDGCHC 2 cut(s) 661, 1356
MluCI AATT 8 cut(s) 231, 518, 900, 993, 1137, 1302, 1437, 1458
MmeI TCCRAC 1 cut(s) 1576
Mph1103I ATGCAT 1 cut(s) 955
MroXI GAANNNNTTC 1 cut(s) 57
MseI TTAA 6 cut(s) 324, 635, 983, 1449, 1527, 1539
MslI CAYNNNNRTG 1 cut(s) 1279
MspA1I CMGCKG 1 cut(s) 26
MspI CCGG 2 cut(s) 579, 1156
MspR9I CCNGG 2 cut(s) 293, 1156
MunI CAATTG 2 cut(s) 993, 1437
Mva1269I GAATGC 1 cut(s) 953
MvaI CCWGG 1 cut(s) 293
MwoI GCNNNNNNNGC 3 cut(s) 113, 434, 1398
NciI CCSGG 1 cut(s) 1156
NdeI CATATG 1 cut(s) 141
NdeII GATC 8 cut(s) 36, 97, 340, 1021, 1109, 1294, 1558, 1636
NlaIV GGNNCC 2 cut(s) 436, 1184
NmuCI GTSAC 1 cut(s) 770
NsiI ATGCAT 1 cut(s) 955
NspI RCATGY 4 cut(s) 308, 673, 1382, 1595
NspV TTCGAA 1 cut(s) 966
PaeR7I CTCGAG 1 cut(s) 1548
PciI ACATGT 1 cut(s) 669
PciSI GCTCTTC 1 cut(s) 363
PcsI WCGNNNNNNNCGW 2 cut(s) 963, 972
PctI GAATGC 1 cut(s) 953
PdmI GAANNNNTTC 1 cut(s) 57
PfeI GAWTC 1 cut(s) 29
PflMI CCANNNNNTGG 1 cut(s) 502
PinAI ACCGGT 1 cut(s) 578
PkrI GCNGC 7 cut(s) 25, 202, 493, 915, 1231, 1510, 1546
Ppu21I YACGTR 1 cut(s) 886
PscI ACATGT 1 cut(s) 669
PsiI TTATAA 2 cut(s) 779, 1262
Psp6I CCWGG 1 cut(s) 291
PspFI CCCAGC 1 cut(s) 1349
PspGI CCWGG 1 cut(s) 291
PspN4I GGNNCC 2 cut(s) 436, 1184
PspXI VCTCGAGB 1 cut(s) 1548
PstNI CAGNNNCTG 1 cut(s) 26
PvuII CAGCTG 1 cut(s) 26
RsaI GTAC 1 cut(s) 961
RsaNI GTAC 1 cut(s) 960
RseI CAYNNNNRTG 1 cut(s) 1279
SapI GCTCTTC 1 cut(s) 363
SaqAI TTAA 6 cut(s) 324, 635, 983, 1449, 1527, 1539
SatI GCNGC 7 cut(s) 24, 201, 492, 914, 1230, 1509, 1545
Sau3AI GATC 8 cut(s) 36, 97, 340, 1021, 1109, 1294, 1558, 1636
ScrFI CCNGG 2 cut(s) 293, 1156
SduI GDGCHC 2 cut(s) 661, 1356
SfaNI GCATC 8 cut(s) 187, 226, 415, 940, 997, 1184, 1241, 1401
SfcI CTRYAG 1 cut(s) 642
Sfr274I CTCGAG 1 cut(s) 1548
SfuI TTCGAA 1 cut(s) 966
SlaI CTCGAG 1 cut(s) 1548
SmiMI CAYNNNNRTG 1 cut(s) 1279
SmlI CTYRAG 3 cut(s) 132, 917, 1548
SmoI CTYRAG 3 cut(s) 132, 917, 1548
Sse9I AATT 8 cut(s) 231, 518, 900, 993, 1137, 1302, 1437, 1458
SsiI CCGC 1 cut(s) 1229
SspI AATATT 1 cut(s) 1618
SspMI CTAG 7 cut(s) 84, 524, 620, 797, 1011, 1329, 1389
StyD4I CCNGG 2 cut(s) 291, 1154
TaaI ACNGT 3 cut(s) 643, 931, 1629
TaiI ACGT 2 cut(s) 301, 888
TaqI TCGA 6 cut(s) 367, 826, 966, 1309, 1395, 1549
TasI AATT 8 cut(s) 231, 518, 900, 993, 1137, 1302, 1437, 1458
TauI GCSGC 1 cut(s) 1232
TfiI GAWTC 1 cut(s) 29
Tru1I TTAA 6 cut(s) 324, 635, 983, 1449, 1527, 1539
Tru9I TTAA 6 cut(s) 324, 635, 983, 1449, 1527, 1539
TscAI CASTG 6 cut(s) 199, 752, 775, 1510, 1632, 1647
TseFI GTSAC 1 cut(s) 770
TseI GCWGC 6 cut(s) 23, 200, 491, 913, 1508, 1544
Tsp45I GTSAC 1 cut(s) 770
TspDTI ATGAA 8 cut(s) 93, 702, 717, 724, 1092, 1227, 1421, 1425
TspGWI ACGGA 1 cut(s) 1119
TspRI CASTG 6 cut(s) 199, 752, 775, 1510, 1632, 1647
Van91I CCANNNNNTGG 1 cut(s) 502
XapI RAATTY 2 cut(s) 900, 1302
XceI RCATGY 4 cut(s) 308, 673, 1382, 1595
XhoI CTCGAG 1 cut(s) 1548
XmiI GTMKAC 2 cut(s) 301, 1245
XmnI GAANNNNTTC 1 cut(s) 57
XspI CTAG 7 cut(s) 84, 524, 620, 797, 1011, 1329, 1389
Zsp2I ATGCAT 1 cut(s) 955
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.