FvH4_4g27943

(-)-germacrene D synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000053
Physical Location & Seq
Reverse (-)
28736947 .. 28739608
2662 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27943.t1

Sequence Viewer

Length: 1695 bp
ATGGCTCTTCATCAAGTTTTAGCAACTCCAGCTCAAACCCCGCATGCTGCTTTCAATGTTGAACCACGTCGTGCTATTTTTGCAGCTCCTTGCTTATTGGGCGATCATTTTCTCTCATATTCTACTATGGATGTAGACGTCAAACTCGAGCAACGTGTACAAGGACTGAAAGAAGAAGTGAAAGGAATGCTAATGACTGCAGTTAACCAGCCTGCTTCGCACATGTTAGACATGGTTGATAACATTCAGCGTTTAGGCTTGTTGTACTGTTTTGAAAATGAGATTGATACAATCTTGAAACATGTTCATGATCATTCTTATGGACGTAAAGATCTTGAAAATAATGGTGACCTTTACACTACTGCTCTCCGTTTTCGATTGCTTAGGCAACAAGGCTATAATGTTTCATGCGATGTGTTCGACAAGTTCAAGGAAAGTAATGGGACATTTAAGGAACATCTTACGAGCGATGTGGTAGGACTACTAAGCCTGTATGAAGCCACTCACCTTAGGGTTCACGGAGAAGACATACTTGAAGAGGCACTGACCTTCACCACGAGCCATCTCGAATCTGCAGTACCCCGGTTGAGCTCCCCTTTGTCGAAACAAGTAACTCATGCCTTGTATCAGCCACTTTGGAAGGGATTTTCAAGGCTAGAAGCCAGGCATTACATGTCTGTCTATGAGGAAGATGATTCACACAACCAAACTCTGTTAACTCTTGCAAAGTTGGATTTCAATTTTGCACAGAAAGTCCATCAGAAAGAACTAAGTGATATGACAAGGTGGTGGAAGGACTTAGACTTTGTAAACAAGCTACCTTTTGCAAGAGACAGGTTAGTTGAGGGCTACTTCTGGGGTTTGGCGACATGTTATGAACCTGAATATTACTTTGCTAGATTTATGGCGTGCAAATATATTGCTTTAACGGCAGTCCTTGACGACGTTTACGATGTCTACGGCACATACGAAGAATTAGAGCGCTTTACAGAAGCTATTGAGCGGTGGGACATTTCTGCTGTTGATCAACTGCAATTGCCCCAGTGTATGGAGGTTTGCTATAAGGGATTGTTGGATAGCTACTCAGGATATGAAGAAAAGCTTGCAGATGAAGGAAACTTGTATCGCATTGAGTTTGCTAAAGAAGCAATCAAAATTCAACTCAGGGGTTACTTCCAGGAAGCCAAATGGTTGAAGGAAAAATACACGCCAACAATGGATGAATATATTCCAAATGGACTTAATTCATCCTTCTATCCGATGGCAATCACAGCCTTTGTTGGAATGGGAGGTCTTGTTACAAAAGACACCATGGATTGGGTGTTAAATCACCCTAAGATTGTTAAAGCTACAGCGCTAATTGGCAGACTCCTCAACGACATTGCTGGCCACAAGTTTGAGCAAAACAACGAGCAGGGTGCCACATCAGTAGAATGCTACATGAACCAATATGGCGTCGGAGAAGAAGAAGCGAAAATCGCGCTAACTAAGCAAACAGATGCTGCATGGAAAGACCTAAACCAAGAGTTGCTCGATCTCCACTCCAGCAATAGTATCCCCAAGCCCCTCCTGCAGATTATTTTGAATCTTGCACGTTCTGGTGAAGTTGTTTACAGGAATAACGAAGGGTTTACCAACTCATCTGCGCTCAAGGGTTTCATAGATTCTCTCCTTATAGAACCAGTGCCCGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

565

Amino Acids

64.53

Weight (kDa)

5.18

Isoelectric Point (pI)

36.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 34 - 208 3.8e-59 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 265 - 505 1e-86 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 305 - 505 1.6e-52 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 141
AccB1I GGYRCC 1 cut(s) 1418
AccB7I CCANNNNNTGG 2 cut(s) 1048, 1317
AccBSI CCGCTC 1 cut(s) 1003
AccI GTMKAC 2 cut(s) 135, 957
AccII CGCG 1 cut(s) 1481
AciI CCGC 2 cut(s) 41, 1003
AcoI YGGCCR 1 cut(s) 1387
AcsI RAATTY 1 cut(s) 1155
AcyI GRCGYC 2 cut(s) 138, 1455
AdeI CACNNNGTG 1 cut(s) 71
AfaI GTAC 3 cut(s) 159, 266, 579
AfeI AGCGCT 2 cut(s) 983, 1356
AfiI CCNNNNNNNGG 2 cut(s) 1048, 1317
AflIII ACRYGT 5 cut(s) 154, 222, 301, 672, 869
AjiI CACGTC 1 cut(s) 68
AjnI CCWGG 2 cut(s) 662, 1176
AluBI AGCT 8 cut(s) 32, 86, 591, 817, 995, 1080, 1102, 1349
AluI AGCT 8 cut(s) 32, 86, 591, 817, 995, 1080, 1102, 1349
Alw21I GWGCWC 1 cut(s) 593
Alw26I GTCTC 1 cut(s) 825
AlwNI CAGNNNCTG 1 cut(s) 1502
Ama87I CYCGRG 1 cut(s) 146
Aor51HI AGCGCT 2 cut(s) 983, 1356
AoxI GGCC 1 cut(s) 1387
ApeKI GCWGC 3 cut(s) 47, 83, 1502
ApoI RAATTY 1 cut(s) 1155
ArsI GACNNNNNNTTYG 2 cut(s) 788, 820
Asp700I GAANNNNTTC 1 cut(s) 1227
AspLEI GCGC 4 cut(s) 984, 1357, 1483, 1648
AsuC2I CCSGG 1 cut(s) 583
AsuHPI GGTGA 5 cut(s) 359, 497, 544, 1322, 1613
AvaI CYCGRG 1 cut(s) 146
AxyI CCTNAGG 1 cut(s) 509
BaeGI GKGCMC 1 cut(s) 1689
BalI TGGCCA 1 cut(s) 1389
BanI GGYRCC 1 cut(s) 1418
BanII GRGCYC 1 cut(s) 593
BarI GAAGNNNNNNTAC 2 cut(s) 1187, 1219
BauI CACGAG 1 cut(s) 556
BbsI GAAGAC 1 cut(s) 531
Bbv12I GWGCWC 1 cut(s) 593
BbvI GCAGC 3 cut(s) 34, 95, 1489
BccI CCATC 3 cut(s) 570, 765, 1255
BceAI ACGGC 2 cut(s) 945, 976
BcgI CGANNNNNNTGC 2 cut(s) 1400, 1434
BciT130I CCWGG 2 cut(s) 664, 1178
BciVI GTATCC 1 cut(s) 1565
BclI TGATCA 2 cut(s) 310, 1024
BcnI CCSGG 1 cut(s) 583
BcoDI GTCTC 1 cut(s) 825
BfaI CTAG 2 cut(s) 656, 897
BfmI CTRYAG 4 cut(s) 198, 573, 1350, 1571
BfoI RGCGCY 2 cut(s) 985, 1358
BfuI GTATCC 1 cut(s) 1565
BglII AGATCT 1 cut(s) 331
BisI GCNGC 3 cut(s) 48, 84, 1503
BlsI GCNGC 3 cut(s) 49, 85, 1504
Bme1390I CCNGG 3 cut(s) 583, 664, 1178
BmeT110I CYCGRG 1 cut(s) 146
BmgBI CACGTC 1 cut(s) 68
BmiI GGNNCC 1 cut(s) 1420
BmrFI CCNGG 3 cut(s) 583, 664, 1178
BmrI ACTGGG 1 cut(s) 1036
BmsI GCATC 1 cut(s) 1489
BmuI ACTGGG 1 cut(s) 1036
BpiI GAAGAC 1 cut(s) 531
BpmI CTGGAG 2 cut(s) 12, 1528
Bpu10I CCTNAGC 1 cut(s) 383
BpuEI CTTGAG 1 cut(s) 1634
BpuMI CCSGG 1 cut(s) 583
BsaBI GATNNNNATC 1 cut(s) 1265
BsaHI GRCGYC 2 cut(s) 138, 1455
BsaJI CCNNGG 2 cut(s) 581, 1311
BsaXI ACNNNNNCTCC 2 cut(s) 1281, 1311
Bsc4I CCNNNNNNNGG 2 cut(s) 1048, 1317
Bse1I ACTGG 2 cut(s) 1042, 1682
Bse21I CCTNAGG 1 cut(s) 509
Bse3DI GCAATG 1 cut(s) 1380
Bse8I GATNNNNATC 1 cut(s) 1265
BseBI CCWGG 2 cut(s) 664, 1178
BseDI CCNNGG 2 cut(s) 581, 1311
BseGI GGATG 3 cut(s) 136, 1225, 1247
BseJI GATNNNNATC 1 cut(s) 1265
BseLI CCNNNNNNNGG 2 cut(s) 1048, 1317
BseMI GCAATG 1 cut(s) 1380
BseMII CTCAG 2 cut(s) 1098, 1177
BseNI ACTGG 2 cut(s) 1042, 1682
BseRI GAGGAG 1 cut(s) 1361
BseSI GKGCMC 1 cut(s) 1689
BseXI GCAGC 3 cut(s) 34, 95, 1489
Bsh1236I CGCG 1 cut(s) 1481
BshFI GGCC 1 cut(s) 1389
BshNI GGYRCC 1 cut(s) 1418
BsiHKAI GWGCWC 1 cut(s) 593
BsiHKCI CYCGRG 1 cut(s) 146
BsiSI CCGG 1 cut(s) 583
BslFI GGGAC 2 cut(s) 457, 1022
BslI CCNNNNNNNGG 2 cut(s) 1048, 1317
BsmAI GTCTC 1 cut(s) 825
BsmFI GGGAC 2 cut(s) 457, 1022
BsmI GAATGC 2 cut(s) 192, 1439
BsnI GGCC 1 cut(s) 1389
BsoBI CYCGRG 1 cut(s) 146
Bsp1286I GDGCHC 2 cut(s) 593, 1689
Bsp1407I TGTACA 1 cut(s) 157
Bsp143I GATC 5 cut(s) 103, 310, 331, 1024, 1534
Bsp19I CCATGG 1 cut(s) 1311
BspACI CCGC 2 cut(s) 41, 1003
BspANI GGCC 1 cut(s) 1389
BspCNI CTCAG 2 cut(s) 1097, 1176
BspFNI CGCG 1 cut(s) 1481
BspHI TCATGA 1 cut(s) 307
BspLI GGNNCC 1 cut(s) 1420
BspMAI CTGCAG 3 cut(s) 202, 577, 1575
BspQI GCTCTTC 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 1418
BsrBI CCGCTC 1 cut(s) 1003
BsrDI GCAATG 1 cut(s) 1380
BsrGI TGTACA 1 cut(s) 157
BsrI ACTGG 2 cut(s) 1042, 1682
BssECI CCNNGG 2 cut(s) 581, 1311
BssMI GATC 5 cut(s) 103, 310, 331, 1024, 1534
BssNI GRCGYC 2 cut(s) 138, 1455
BssSI CACGAG 1 cut(s) 556
BssT1I CCWWGG 1 cut(s) 1311
Bst2BI CACGAG 1 cut(s) 556
Bst2UI CCWGG 2 cut(s) 664, 1178
Bst4CI ACNGT 1 cut(s) 269
Bst6I CTCTTC 2 cut(s) 12, 531
BstACI GRCGYC 2 cut(s) 138, 1455
BstAUI TGTACA 1 cut(s) 157
BstC8I GCNNGC 5 cut(s) 45, 213, 910, 1104, 1387
BstDEI CTNAG 9 cut(s) 383, 485, 509, 770, 799, 1084, 1163, 1335, 1488
BstDSI CCRYGG 1 cut(s) 1311
BstEII GGTNACC 1 cut(s) 347
BstF5I GGATG 3 cut(s) 136, 1225, 1247
BstFNI CGCG 1 cut(s) 1481
BstH2I RGCGCY 2 cut(s) 985, 1358
BstHHI GCGC 4 cut(s) 984, 1357, 1483, 1648
BstKTI GATC 5 cut(s) 106, 313, 334, 1027, 1537
BstMAI GTCTC 1 cut(s) 825
BstMBI GATC 5 cut(s) 103, 310, 331, 1024, 1534
BstNI CCWGG 2 cut(s) 664, 1178
BstNSI RCATGY 5 cut(s) 47, 226, 305, 676, 873
BstPI GGTNACC 1 cut(s) 347
BstSCI CCNGG 3 cut(s) 581, 662, 1176
BstSFI CTRYAG 4 cut(s) 198, 573, 1350, 1571
BstSLI GKGCMC 1 cut(s) 1689
BstUI CGCG 1 cut(s) 1481
BstV1I GCAGC 3 cut(s) 34, 95, 1489
BstV2I GAAGAC 1 cut(s) 531
BstX2I RGATCY 1 cut(s) 331
BstYI RGATCY 1 cut(s) 331
Bsu36I CCTNAGG 1 cut(s) 509
BsuI GTATCC 1 cut(s) 1565
BsuRI GGCC 1 cut(s) 1389
BtgI CCRYGG 1 cut(s) 1311
BtgZI GCGATG 2 cut(s) 426, 483
BtrI CACGTC 1 cut(s) 68
BtsCI GGATG 3 cut(s) 136, 1225, 1247
BtsIMutI CAGTG 3 cut(s) 542, 1049, 1689
Cac8I GCNNGC 5 cut(s) 45, 213, 910, 1104, 1387
CaiI CAGNNNCTG 1 cut(s) 1502
CciI TCATGA 1 cut(s) 307
CfoI GCGC 4 cut(s) 984, 1357, 1483, 1648
CseI GACGC 1 cut(s) 1444
Csp6I GTAC 3 cut(s) 158, 265, 578
CviQI GTAC 3 cut(s) 158, 265, 578
DdeI CTNAG 9 cut(s) 383, 485, 509, 770, 799, 1084, 1163, 1335, 1488
DpnI GATC 5 cut(s) 105, 312, 333, 1026, 1536
DpnII GATC 5 cut(s) 103, 310, 331, 1024, 1534
DraIII CACNNNGTG 1 cut(s) 71
EaeI YGGCCR 1 cut(s) 1387
Eam1104I CTCTTC 2 cut(s) 12, 531
EarI CTCTTC 2 cut(s) 12, 531
Ecl136II GAGCTC 1 cut(s) 591
Eco130I CCWWGG 1 cut(s) 1311
Eco24I GRGCYC 1 cut(s) 593
Eco47III AGCGCT 2 cut(s) 983, 1356
Eco53kI GAGCTC 1 cut(s) 591
Eco81I CCTNAGG 1 cut(s) 509
Eco88I CYCGRG 1 cut(s) 146
Eco91I GGTNACC 1 cut(s) 347
EcoICRI GAGCTC 1 cut(s) 591
EcoO65I GGTNACC 1 cut(s) 347
EcoRII CCWGG 2 cut(s) 662, 1176
EcoT14I CCWWGG 1 cut(s) 1311
EcoT38I GRGCYC 1 cut(s) 593
ErhI CCWWGG 1 cut(s) 1311
FalI AAGNNNNNCTT 4 cut(s) 516, 548, 1086, 1118
FaqI GGGAC 2 cut(s) 457, 1022
FauI CCCGC 1 cut(s) 48
FbaI TGATCA 2 cut(s) 310, 1024
FblI GTMKAC 2 cut(s) 135, 957
Fnu4HI GCNGC 3 cut(s) 48, 84, 1503
FokI GGATG 3 cut(s) 143, 1232, 1234
FriOI GRGCYC 1 cut(s) 593
Fsp4HI GCNGC 3 cut(s) 48, 84, 1503
FspBI CTAG 2 cut(s) 656, 897
GlaI GCGC 4 cut(s) 983, 1356, 1482, 1647
GluI GCNGC 3 cut(s) 48, 84, 1503
GsuI CTGGAG 2 cut(s) 12, 1528
HaeII RGCGCY 2 cut(s) 985, 1358
HaeIII GGCC 1 cut(s) 1389
HapII CCGG 1 cut(s) 583
HgaI GACGC 1 cut(s) 1444
HhaI GCGC 4 cut(s) 984, 1357, 1483, 1648
Hin1I GRCGYC 2 cut(s) 138, 1455
Hin6I GCGC 4 cut(s) 982, 1355, 1481, 1646
HinP1I GCGC 4 cut(s) 982, 1355, 1481, 1646
HincII GTYRAC 2 cut(s) 205, 717
HindII GTYRAC 2 cut(s) 205, 717
HindIII AAGCTT 1 cut(s) 1100
HinfI GANTC 5 cut(s) 569, 695, 1368, 1585, 1664
HpaI GTTAAC 2 cut(s) 205, 717
HpaII CCGG 1 cut(s) 583
HphI GGTGA 5 cut(s) 359, 497, 544, 1322, 1613
Hpy188I TCNGA 3 cut(s) 762, 1260, 1460
Hpy188III TCNNGA 5 cut(s) 295, 308, 335, 566, 1086
Hpy99I CGWCG 3 cut(s) 72, 947, 1460
HpyAV CCTTC 7 cut(s) 559, 634, 787, 1106, 1189, 1261, 1619
HpyCH4III ACNGT 1 cut(s) 269
HpyCH4IV ACGT 6 cut(s) 67, 138, 154, 325, 945, 1594
HpyF3I CTNAG 9 cut(s) 383, 485, 509, 770, 799, 1084, 1163, 1335, 1488
HpySE526I ACGT 6 cut(s) 67, 138, 154, 325, 945, 1594
Hsp92I GRCGYC 2 cut(s) 138, 1455
HspAI GCGC 4 cut(s) 982, 1355, 1481, 1646
Ksp22I TGATCA 2 cut(s) 310, 1024
KspAI GTTAAC 2 cut(s) 205, 717
Kzo9I GATC 5 cut(s) 103, 310, 331, 1024, 1534
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 2 cut(s) 91, 596
Lsp1109I GCAGC 3 cut(s) 34, 95, 1489
LweI GCATC 1 cut(s) 1489
MaeI CTAG 2 cut(s) 656, 897
MaeII ACGT 6 cut(s) 67, 138, 154, 325, 945, 1594
MaeIII GTNAC 4 cut(s) 347, 610, 1169, 1297
MalI GATC 5 cut(s) 105, 312, 333, 1026, 1536
MbiI CCGCTC 1 cut(s) 1003
MboI GATC 5 cut(s) 103, 310, 331, 1024, 1534
MboII GAAGA 8 cut(s) 185, 536, 548, 701, 983, 1106, 1475, 1478
MfeI CAATTG 1 cut(s) 1034
MflI RGATCY 1 cut(s) 331
MhlI GDGCHC 2 cut(s) 593, 1689
MlsI TGGCCA 1 cut(s) 1389
MluCI AATT 6 cut(s) 739, 974, 1034, 1155, 1243, 1359
MluNI TGGCCA 1 cut(s) 1389
MlyI GAGTC 1 cut(s) 1362
MmeI TCCRAC 4 cut(s) 711, 1053, 1261, 1438
MnlI CCTC 7 cut(s) 532, 679, 838, 1045, 1283, 1382, 1577
Mox20I TGGCCA 1 cut(s) 1389
MroXI GAANNNNTTC 1 cut(s) 1227
MscI TGGCCA 1 cut(s) 1389
MseI TTAA 8 cut(s) 204, 450, 716, 926, 1242, 1325, 1344, 1693
MslI CAYNNNNRTG 2 cut(s) 306, 318
Msp20I TGGCCA 1 cut(s) 1389
MspI CCGG 1 cut(s) 583
MspR9I CCNGG 3 cut(s) 583, 664, 1178
MunI CAATTG 1 cut(s) 1034
Mva1269I GAATGC 2 cut(s) 192, 1439
MvaI CCWGG 2 cut(s) 664, 1178
MvnI CGCG 1 cut(s) 1481
NciI CCSGG 1 cut(s) 583
NcoI CCATGG 1 cut(s) 1311
NdeII GATC 5 cut(s) 103, 310, 331, 1024, 1534
NlaIV GGNNCC 1 cut(s) 1420
NmuCI GTSAC 1 cut(s) 347
NspI RCATGY 5 cut(s) 47, 226, 305, 676, 873
PaeI GCATGC 1 cut(s) 47
PaeR7I CTCGAG 1 cut(s) 146
PagI TCATGA 1 cut(s) 307
PciI ACATGT 4 cut(s) 222, 301, 672, 869
PciSI GCTCTTC 1 cut(s) 12
PcsI WCGNNNNNNNCGW 3 cut(s) 144, 948, 966
PctI GAATGC 2 cut(s) 192, 1439
PdmI GAANNNNTTC 1 cut(s) 1227
PfeI GAWTC 4 cut(s) 569, 695, 1585, 1664
PflMI CCANNNNNTGG 2 cut(s) 1048, 1317
PfoI TCCNGGA 1 cut(s) 1176
PkrI GCNGC 3 cut(s) 49, 85, 1504
PleI GAGTC 1 cut(s) 1362
PpsI GAGTC 1 cut(s) 1362
PscI ACATGT 4 cut(s) 222, 301, 672, 869
Psp124BI GAGCTC 1 cut(s) 593
Psp6I CCWGG 2 cut(s) 662, 1176
PspEI GGTNACC 1 cut(s) 347
PspGI CCWGG 2 cut(s) 662, 1176
PspN4I GGNNCC 1 cut(s) 1420
PspXI VCTCGAGB 1 cut(s) 146
PstI CTGCAG 3 cut(s) 202, 577, 1575
PstNI CAGNNNCTG 1 cut(s) 1502
PsuI RGATCY 1 cut(s) 331
RsaI GTAC 3 cut(s) 159, 266, 579
RsaNI GTAC 3 cut(s) 158, 265, 578
RseI CAYNNNNRTG 2 cut(s) 306, 318
SacI GAGCTC 1 cut(s) 593
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 8 cut(s) 204, 450, 716, 926, 1242, 1325, 1344, 1693
SatI GCNGC 3 cut(s) 48, 84, 1503
Sau3AI GATC 5 cut(s) 103, 310, 331, 1024, 1534
SchI GAGTC 1 cut(s) 1362
ScrFI CCNGG 3 cut(s) 583, 664, 1178
SduI GDGCHC 2 cut(s) 593, 1689
SfaNI GCATC 1 cut(s) 1489
SfcI CTRYAG 4 cut(s) 198, 573, 1350, 1571
Sfr274I CTCGAG 1 cut(s) 146
SlaI CTCGAG 1 cut(s) 146
SmiMI CAYNNNNRTG 2 cut(s) 306, 318
SmlI CTYRAG 2 cut(s) 146, 1649
SmoI CTYRAG 2 cut(s) 146, 1649
SphI GCATGC 1 cut(s) 47
Sse9I AATT 6 cut(s) 739, 974, 1034, 1155, 1243, 1359
SsiI CCGC 2 cut(s) 41, 1003
SspI AATATT 1 cut(s) 887
SspMI CTAG 2 cut(s) 656, 897
SstI GAGCTC 1 cut(s) 593
StyD4I CCNGG 3 cut(s) 581, 662, 1176
StyI CCWWGG 1 cut(s) 1311
TaaI ACNGT 1 cut(s) 269
TaiI ACGT 6 cut(s) 70, 141, 157, 328, 948, 1597
TaqI TCGA 6 cut(s) 147, 376, 420, 567, 602, 1533
TasI AATT 6 cut(s) 739, 974, 1034, 1155, 1243, 1359
TatI WGTACW 2 cut(s) 157, 264
TfiI GAWTC 4 cut(s) 569, 695, 1585, 1664
Tru1I TTAA 8 cut(s) 204, 450, 716, 926, 1242, 1325, 1344, 1693
Tru9I TTAA 8 cut(s) 204, 450, 716, 926, 1242, 1325, 1344, 1693
TscAI CASTG 3 cut(s) 549, 1049, 1689
TseFI GTSAC 1 cut(s) 347
TseI GCWGC 3 cut(s) 47, 83, 1502
Tsp45I GTSAC 1 cut(s) 347
TspGWI ACGGA 2 cut(s) 359, 534
TspRI CASTG 3 cut(s) 549, 1049, 1689
Van91I CCANNNNNTGG 2 cut(s) 1048, 1317
XapI RAATTY 1 cut(s) 1155
XceI RCATGY 5 cut(s) 47, 226, 305, 676, 873
XhoI CTCGAG 1 cut(s) 146
XmiI GTMKAC 2 cut(s) 135, 957
XmnI GAANNNNTTC 1 cut(s) 1227
XspI CTAG 2 cut(s) 656, 897
ZraI GACGTC 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.