Rroxscaffold_7G00197820

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000053
Physical Location & Seq
Reverse (-)
42408999 .. 42412084
3086 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00197820.1

Sequence Viewer

Length: 1644 bp
ATGTCGTCTACACCAACTAGTTCTGTATCATTTCCGGTACCACAAATTGCAAAGCCTGAAATCATTCGCCGGAGAGCAAATTTTCAACCTAGCATTTGGGGAGATCAATTCATCAACTATGATTCCAAAAAAACTAAGGCTGATGCCCTTTGGCAGCAGCAAGTTGACAAATTGAAAACAGTAGTGAAGAGGGAACTCTTTACTAATGAAGGTGATCTTTCACATCGACTCAAGTTAATTGATGCAATCCAGCGACTTGGGGTGGCATATCATTTTGAACGCGAAATAGAAGAATCACTGCAACATATCCATGCGACATATCGTGATCATGATCTGTACCGTGATGATGGCCATCTTTACGATGTTGCCCTATTATTTCGGCTTCTAAGACAACATGGATATCAAATTTCATCGGATATATTCAACAAGTTCAAAGATTCAAATGGTAGCTTCAAAGAATGCTTAATTTCTGATGTCTCCGGTATGCTAAGCTTCTATGAAGCAACACATCTTAGGGTTAATGGGGAAGATATACTTGAAGAGGCTCTTCTGTTCACCACCACTCAACTTAAGTACGTGCTCAAAAGCAATGTAATAAGCTATCCACCAGTATTGAAGGAACAAATAACTGAAGCTTTGACGAGACCTCTTCGAAAAACTTTGGAGAGATTATGCGCCAAGCGTTATATTTCAATCTACCAAGATGACGCCTCACACAGTGAATCTTTGTTGAAACTTGCAAAGTGGTGGAAAGAACTAGACTTTGAAAGGAAATTGCCTTTTGCAAGAGACAGGGTGGTGGAGGTGTTCTTTTGGATGGTGGGAGTATATTTTGAACCCCAGTACTCTATTGGCAGAATCATTATGACAAAAGTGGGTATCCTCCTAACTATTTTGGATGATATCTATGATGCATATGGTACATTTGAAGAACTCATGATCTTCACTCAAGCAATTGACAGGTGGAATGTCAAATGCATAGATGAACTCCCAGACTATTTGAAAATATTCTATCATGAACTTTTGAATCTTTTTGATGAAATTGACAAATTGATGTCAAAGGAAGGAAGACCATATCGAACTTCATATGCAATAGAAGCTATGAAAGTTCAGGCTCGATCATTTTTCAACGAGACCCAATGGTTGCACGAAGGACGCACTCCGAGCATGGAGGAATACATGAGTGTTGCTACAGTTTCTGTTACTTACACTTTTATAACAACCATTTCATTACTTGGCATGGGAGATATTGTAACGAAGGATGCATTTGAGTGGTTGTTGGATGACCCGAAAATTGTTAGAGCTTCCAATATCATTTTTAGGCTCATGGATGACATTGTTTCCACCAAGTTTGAGAAAGAGAGAGGGCATGCACCTTCTAGCATTGATTGCTATATGAAGCAATATGGTGTTTTAGAACAAGAGACGATTGACGTTTTTGAGAAGCAAATTGTGAAATCATGGAAGGATATAAATGAGGAGTTTCTTAAACCAACCGCTATGCCAATGCCGGTTCTTATGCGTGTTCTCAATTTAACAAGAGTAGTGGATCTTCTTTACAAGGGAGAAGATGGATTCACACGAGTTGGGAAGATGACCAAAGATAGTGTTGCTGCAGTTATTATCGACTCGGTGCCACCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

547

Amino Acids

63.72

Weight (kDa)

5.69

Isoelectric Point (pI)

42.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 32 - 213 8.3e-58 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 251 - 490 1.9e-95 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 291 - 490 8.9e-55 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1217
Acc65I GGTACC 1 cut(s) 37
AccB1I GGYRCC 2 cut(s) 37, 1633
AccI GTMKAC 1 cut(s) 8
AccII CGCG 1 cut(s) 282
AciI CCGC 1 cut(s) 1497
AclWI GGATC 1 cut(s) 1557
AcoI YGGCCR 1 cut(s) 349
AcsI RAATTY 2 cut(s) 79, 405
AcuI CTGAAG 1 cut(s) 651
AcyI GRCGYC 1 cut(s) 708
AdeI CACNNNGTG 1 cut(s) 719
AfaI GTAC 5 cut(s) 39, 338, 575, 845, 922
AflII CTTAAG 1 cut(s) 569
AhlI ACTAGT 1 cut(s) 17
AluBI AGCT 6 cut(s) 450, 492, 600, 635, 1100, 1304
AluI AGCT 6 cut(s) 450, 492, 600, 635, 1100, 1304
Alw21I GWGCWC 1 cut(s) 582
Alw26I GTCTC 5 cut(s) 481, 637, 783, 1127, 1418
AlwI GGATC 1 cut(s) 1557
AlwNI CAGNNNCTG 1 cut(s) 1199
AoxI GGCC 1 cut(s) 349
ApeKI GCWGC 3 cut(s) 154, 157, 1613
ApoI RAATTY 2 cut(s) 79, 405
Asp700I GAANNNNTTC 2 cut(s) 63, 1007
Asp718I GGTACC 1 cut(s) 37
AspLEI GCGC 1 cut(s) 677
AsuHPI GGTGA 2 cut(s) 224, 547
AsuII TTCGAA 1 cut(s) 652
BalI TGGCCA 1 cut(s) 351
BanI GGYRCC 2 cut(s) 37, 1633
BarI GAAGNNNNNNTAC 2 cut(s) 1536, 1568
BauI CACGAG 1 cut(s) 1581
BbsI GAAGAC 1 cut(s) 1075
Bbv12I GWGCWC 1 cut(s) 582
BbvI GCAGC 3 cut(s) 166, 169, 1600
BccI CCATC 4 cut(s) 341, 360, 811, 1565
BciVI GTATCC 1 cut(s) 890
BclI TGATCA 1 cut(s) 325
BcoDI GTCTC 5 cut(s) 481, 637, 783, 1127, 1418
BcuI ACTAGT 1 cut(s) 17
BfaI CTAG 4 cut(s) 18, 90, 758, 1380
BfmI CTRYAG 2 cut(s) 1191, 1614
BfrI CTTAAG 1 cut(s) 569
BfuI GTATCC 1 cut(s) 890
BisI GCNGC 3 cut(s) 155, 158, 1614
BlpI GCTNAGC 1 cut(s) 488
BlsI GCNGC 3 cut(s) 156, 159, 1615
BmcAI AGTACT 1 cut(s) 845
BmiI GGNNCC 2 cut(s) 39, 1635
BmrI ACTGGG 1 cut(s) 835
BmsI GCATC 4 cut(s) 133, 232, 901, 1252
BmuI ACTGGG 1 cut(s) 835
BpiI GAAGAC 1 cut(s) 1075
Bpu1102I GCTNAGC 1 cut(s) 488
Bpu14I TTCGAA 1 cut(s) 652
BpuEI CTTGAG 2 cut(s) 215, 933
BsaAI YACGTR 1 cut(s) 577
BsaBI GATNNNNATC 2 cut(s) 330, 351
BsaHI GRCGYC 1 cut(s) 708
BsaI GGTCTC 2 cut(s) 637, 1127
BsaWI WCCGGW 2 cut(s) 34, 479
BsaXI ACNNNNNCTCC 2 cut(s) 1236, 1266
Bse118I RCCGGY 1 cut(s) 1510
Bse1I ACTGG 2 cut(s) 608, 841
Bse3DI GCAATG 1 cut(s) 595
Bse8I GATNNNNATC 2 cut(s) 330, 351
BseGI GGATG 5 cut(s) 822, 904, 1267, 1288, 1336
BseJI GATNNNNATC 2 cut(s) 330, 351
BseMI GCAATG 1 cut(s) 595
BseNI ACTGG 2 cut(s) 608, 841
BseRI GAGGAG 1 cut(s) 1493
BseXI GCAGC 3 cut(s) 166, 169, 1600
Bsh1236I CGCG 1 cut(s) 282
BshFI GGCC 1 cut(s) 351
BshNI GGYRCC 2 cut(s) 37, 1633
BsiHKAI GWGCWC 1 cut(s) 582
BsiSI CCGG 4 cut(s) 35, 70, 480, 1511
BsmAI GTCTC 5 cut(s) 481, 637, 783, 1127, 1418
BsmBI CGTCTC 1 cut(s) 1418
BsmI GAATGC 1 cut(s) 464
BsnI GGCC 1 cut(s) 351
Bso31I GGTCTC 2 cut(s) 637, 1127
Bsp119I TTCGAA 1 cut(s) 652
Bsp1286I GDGCHC 1 cut(s) 582
Bsp143I GATC 7 cut(s) 103, 214, 325, 331, 939, 1118, 1549
Bsp1720I GCTNAGC 1 cut(s) 488
BspACI CCGC 1 cut(s) 1497
BspANI GGCC 1 cut(s) 351
BspFNI CGCG 1 cut(s) 282
BspHI TCATGA 3 cut(s) 328, 936, 1015
BspLI GGNNCC 2 cut(s) 39, 1635
BspMAI CTGCAG 1 cut(s) 1618
BspPI GGATC 1 cut(s) 1557
BspQI GCTCTTC 1 cut(s) 552
BspT104I TTCGAA 1 cut(s) 652
BspT107I GGYRCC 2 cut(s) 37, 1633
BspTI CTTAAG 1 cut(s) 569
BspTNI GGTCTC 2 cut(s) 637, 1127
BsrDI GCAATG 1 cut(s) 595
BsrFI RCCGGY 1 cut(s) 1510
BsrI ACTGG 2 cut(s) 608, 841
BssAI RCCGGY 1 cut(s) 1510
BssMI GATC 7 cut(s) 103, 214, 325, 331, 939, 1118, 1549
BssNI GRCGYC 1 cut(s) 708
BssSI CACGAG 1 cut(s) 1581
Bst2BI CACGAG 1 cut(s) 1581
Bst4CI ACNGT 5 cut(s) 181, 341, 719, 1195, 1641
Bst6I CTCTTC 4 cut(s) 182, 534, 552, 654
BstACI GRCGYC 1 cut(s) 708
BstAFI CTTAAG 1 cut(s) 569
BstAPI GCANNNNNTGC 1 cut(s) 1389
BstBAI YACGTR 1 cut(s) 577
BstBI TTCGAA 1 cut(s) 652
BstC8I GCNNGC 1 cut(s) 1371
BstDEI CTNAG 4 cut(s) 135, 386, 488, 512
BstF5I GGATG 5 cut(s) 822, 904, 1267, 1288, 1336
BstFNI CGCG 1 cut(s) 282
BstHHI GCGC 1 cut(s) 677
BstKTI GATC 7 cut(s) 106, 217, 328, 334, 942, 1121, 1552
BstMAI GTCTC 5 cut(s) 481, 637, 783, 1127, 1418
BstMBI GATC 7 cut(s) 103, 214, 325, 331, 939, 1118, 1549
BstMWI GCNNNNNNNGC 3 cut(s) 1097, 1164, 1389
BstNSI RCATGY 1 cut(s) 1373
BstSFI CTRYAG 2 cut(s) 1191, 1614
BstUI CGCG 1 cut(s) 282
BstV1I GCAGC 3 cut(s) 166, 169, 1600
BstV2I GAAGAC 1 cut(s) 1075
BstX2I RGATCY 1 cut(s) 1549
BstXI CCANNNNNNTGG 1 cut(s) 257
BstYI RGATCY 1 cut(s) 1549
BsuI GTATCC 1 cut(s) 890
BsuRI GGCC 1 cut(s) 351
BtsCI GGATG 5 cut(s) 822, 904, 1267, 1288, 1336
BtsI GCAGTG 1 cut(s) 296
BtsIMutI CAGTG 2 cut(s) 296, 724
Cac8I GCNNGC 1 cut(s) 1371
CaiI CAGNNNCTG 1 cut(s) 1199
CciI TCATGA 3 cut(s) 328, 936, 1015
CfoI GCGC 1 cut(s) 677
Cfr10I RCCGGY 1 cut(s) 1510
CseI GACGC 2 cut(s) 716, 1164
Csp6I GTAC 5 cut(s) 38, 337, 574, 844, 921
CspCI CAANNNNNGTGG 6 cut(s) 594, 629, 1333, 1368, 1527, 1562
CviQI GTAC 5 cut(s) 38, 337, 574, 844, 921
DdeI CTNAG 4 cut(s) 135, 386, 488, 512
DpnI GATC 7 cut(s) 105, 216, 327, 333, 941, 1120, 1551
DpnII GATC 7 cut(s) 103, 214, 325, 331, 939, 1118, 1549
DraIII CACNNNGTG 1 cut(s) 719
EaeI YGGCCR 1 cut(s) 349
Eam1104I CTCTTC 4 cut(s) 182, 534, 552, 654
EarI CTCTTC 4 cut(s) 182, 534, 552, 654
Eco31I GGTCTC 2 cut(s) 637, 1127
Eco32I GATATC 2 cut(s) 401, 904
Eco57I CTGAAG 1 cut(s) 651
EcoRV GATATC 2 cut(s) 401, 904
EcoT22I ATGCAT 3 cut(s) 916, 980, 1267
Esp3I CGTCTC 1 cut(s) 1418
FalI AAGNNNNNCTT 6 cut(s) 201, 233, 519, 551, 531, 563
FauNDI CATATG 2 cut(s) 916, 1087
FbaI TGATCA 1 cut(s) 325
FblI GTMKAC 1 cut(s) 8
Fnu4HI GCNGC 3 cut(s) 155, 158, 1614
FokI GGATG 5 cut(s) 829, 911, 1274, 1295, 1343
Fsp4HI GCNGC 3 cut(s) 155, 158, 1614
FspBI CTAG 4 cut(s) 18, 90, 758, 1380
GlaI GCGC 1 cut(s) 676
GluI GCNGC 3 cut(s) 155, 158, 1614
HaeIII GGCC 1 cut(s) 351
HapII CCGG 4 cut(s) 35, 70, 480, 1511
HgaI GACGC 2 cut(s) 716, 1164
HhaI GCGC 1 cut(s) 677
Hin1I GRCGYC 1 cut(s) 708
Hin6I GCGC 1 cut(s) 675
HinP1I GCGC 1 cut(s) 675
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HindIII AAGCTT 2 cut(s) 490, 633
HinfI GANTC 9 cut(s) 122, 228, 293, 437, 722, 858, 1027, 1575, 1628
HpaII CCGG 4 cut(s) 35, 70, 480, 1511
HphI GGTGA 2 cut(s) 224, 547
Hpy166II GTNNAC 3 cut(s) 9, 166, 555
Hpy188I TCNGA 3 cut(s) 415, 472, 1164
Hpy188III TCNNGA 4 cut(s) 323, 329, 937, 1016
Hpy8I GTNNAC 3 cut(s) 9, 166, 555
HpyAV CCTTC 7 cut(s) 203, 610, 1058, 1145, 1252, 1386, 1459
HpyCH4III ACNGT 5 cut(s) 181, 341, 719, 1195, 1641
HpyCH4IV ACGT 2 cut(s) 576, 1434
HpyF10VI GCNNNNNNNGC 3 cut(s) 1097, 1164, 1389
HpyF3I CTNAG 4 cut(s) 135, 386, 488, 512
HpySE526I ACGT 2 cut(s) 576, 1434
Hsp92I GRCGYC 1 cut(s) 708
HspAI GCGC 1 cut(s) 675
KpnI GGTACC 1 cut(s) 41
Ksp22I TGATCA 1 cut(s) 325
Kzo9I GATC 7 cut(s) 103, 214, 325, 331, 939, 1118, 1549
LguI GCTCTTC 1 cut(s) 552
Lsp1109I GCAGC 3 cut(s) 166, 169, 1600
LweI GCATC 4 cut(s) 133, 232, 901, 1252
MaeI CTAG 4 cut(s) 18, 90, 758, 1380
MaeII ACGT 2 cut(s) 576, 1434
MaeIII GTNAC 2 cut(s) 1201, 1252
MalI GATC 7 cut(s) 105, 216, 327, 333, 941, 1120, 1551
MboI GATC 7 cut(s) 103, 214, 325, 331, 939, 1118, 1549
MfeI CAATTG 1 cut(s) 954
MflI RGATCY 1 cut(s) 1549
MhlI GDGCHC 1 cut(s) 582
MlsI TGGCCA 1 cut(s) 351
MluNI TGGCCA 1 cut(s) 351
MlyI GAGTC 2 cut(s) 222, 1622
MmeI TCCRAC 1 cut(s) 1260
MnlI CCTC 9 cut(s) 183, 535, 657, 721, 796, 893, 1165, 1358, 1471
Mox20I TGGCCA 1 cut(s) 351
Mph1103I ATGCAT 3 cut(s) 916, 980, 1267
MroXI GAANNNNTTC 2 cut(s) 63, 1007
MscI TGGCCA 1 cut(s) 351
MseI TTAA 6 cut(s) 236, 464, 519, 570, 1488, 1535
MslI CAYNNNNRTG 2 cut(s) 309, 1270
Msp20I TGGCCA 1 cut(s) 351
MspCI CTTAAG 1 cut(s) 569
MspI CCGG 4 cut(s) 35, 70, 480, 1511
MunI CAATTG 1 cut(s) 954
Mva1269I GAATGC 1 cut(s) 464
MvnI CGCG 1 cut(s) 282
MwoI GCNNNNNNNGC 3 cut(s) 1097, 1164, 1389
NdeI CATATG 2 cut(s) 916, 1087
NdeII GATC 7 cut(s) 103, 214, 325, 331, 939, 1118, 1549
NlaIV GGNNCC 2 cut(s) 39, 1635
NsiI ATGCAT 3 cut(s) 916, 980, 1267
NspI RCATGY 1 cut(s) 1373
NspV TTCGAA 1 cut(s) 652
PaeI GCATGC 1 cut(s) 1373
PagI TCATGA 3 cut(s) 328, 936, 1015
PciSI GCTCTTC 1 cut(s) 552
PcsI WCGNNNNNNNCGW 1 cut(s) 1637
PctI GAATGC 1 cut(s) 464
PdmI GAANNNNTTC 2 cut(s) 63, 1007
PfeI GAWTC 7 cut(s) 122, 293, 437, 722, 858, 1027, 1575
PkrI GCNGC 3 cut(s) 156, 159, 1615
PleI GAGTC 2 cut(s) 222, 1622
PpsI GAGTC 2 cut(s) 222, 1622
Ppu21I YACGTR 1 cut(s) 577
PsiI TTATAA 1 cut(s) 1217
PspN4I GGNNCC 2 cut(s) 39, 1635
PstI CTGCAG 1 cut(s) 1618
PstNI CAGNNNCTG 1 cut(s) 1199
PsuI RGATCY 1 cut(s) 1549
RsaI GTAC 5 cut(s) 39, 338, 575, 845, 922
RsaNI GTAC 5 cut(s) 38, 337, 574, 844, 921
RseI CAYNNNNRTG 2 cut(s) 309, 1270
SapI GCTCTTC 1 cut(s) 552
SaqAI TTAA 6 cut(s) 236, 464, 519, 570, 1488, 1535
SatI GCNGC 3 cut(s) 155, 158, 1614
Sau3AI GATC 7 cut(s) 103, 214, 325, 331, 939, 1118, 1549
ScaI AGTACT 1 cut(s) 845
SchI GAGTC 2 cut(s) 222, 1622
SduI GDGCHC 1 cut(s) 582
SfaNI GCATC 4 cut(s) 133, 232, 901, 1252
SfcI CTRYAG 2 cut(s) 1191, 1614
SfuI TTCGAA 1 cut(s) 652
SmiMI CAYNNNNRTG 2 cut(s) 309, 1270
SmlI CTYRAG 3 cut(s) 230, 569, 948
SmoI CTYRAG 3 cut(s) 230, 569, 948
SpeI ACTAGT 1 cut(s) 17
SphI GCATGC 1 cut(s) 1373
SsiI CCGC 1 cut(s) 1497
SspI AATATT 1 cut(s) 1008
SspMI CTAG 4 cut(s) 18, 90, 758, 1380
TaaI ACNGT 5 cut(s) 181, 341, 719, 1195, 1641
TaiI ACGT 2 cut(s) 579, 1437
TaqI TCGA 5 cut(s) 226, 652, 1078, 1117, 1626
TatI WGTACW 1 cut(s) 843
TfiI GAWTC 7 cut(s) 122, 293, 437, 722, 858, 1027, 1575
Tru1I TTAA 6 cut(s) 236, 464, 519, 570, 1488, 1535
Tru9I TTAA 6 cut(s) 236, 464, 519, 570, 1488, 1535
TscAI CASTG 2 cut(s) 303, 724
TseI GCWGC 3 cut(s) 154, 157, 1613
TspRI CASTG 2 cut(s) 303, 724
Vha464I CTTAAG 1 cut(s) 569
XapI RAATTY 2 cut(s) 79, 405
XceI RCATGY 1 cut(s) 1373
XcmI CCANNNNNNNNNTGG 1 cut(s) 848
XmiI GTMKAC 1 cut(s) 8
XmnI GAANNNNTTC 2 cut(s) 63, 1007
XspI CTAG 4 cut(s) 18, 90, 758, 1380
ZrmI AGTACT 1 cut(s) 845
Zsp2I ATGCAT 3 cut(s) 916, 980, 1267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.