Rw6G014890

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000053
Physical Location & Seq
Forward (+)
27891962 .. 27896962
5001 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G014890.1

Sequence Viewer

Length: 1677 bp
ATGTCGTCTGTGCCAACTAGTTATGTATCATTTCCAGTACCACAAATTGCAAAGCCTGAAATCATTCGCCGGATAGCAAATTTTCAACCAAGCATTTGGGGAGATCAGTTCATCAACTATGATTCCCAAAAAACTCAGCAAGTTGACAAATTGAAAACAGTAGTAAAGAGGGAACTCTTTACTAATGAAGGTGATTTTTCACATCGACTCAAGTTAATTGATGCAATCCAACGAATTGGCGTTGCATATCATTTTGAACGCGAAATAGAAGAATCATTGCAACATATCCATGCGACATATCGTGATCGGGATCTGTACCGTGATGATGGCCATCTTTACGATGTTGCCCTATTGTTTCGGCTTCTGAGGCAACATGGATATCAAATTTCGTCTGGTATATTCAACAAGTTTAAAGATTCAAATGGTAGCTTCAAAGAATGCTTAATTTCTGATGCCTCCGGTATGCTAAGCCTCTATGAAGCAACACATCTTAGGGTTAATGGAGAAGATATACTTGAAGAGGCTCTTTTGTTCACCACCACTCAACTCAAGTACGTGCTCAAAAGCAATGTAAGCTATCCACCAGTATTGAAGGAACAAATAACTGAAGCTTTGACGAGACCTCTTCGAAAAACTTTGGAGAGATTATGCACCAAGCGTTATATTCCAATCTACCAAGATGAAGCCTCACACAGTGAATCTCTATTGAAACTTGCAAAGTTGGATTTCAATCTTGTTCAGTCTTTACACCAAAAGGAGCTCTCTGATATTACTAGGTGGTGGAAAGAACTAGACTTTGAAAGGAAATTGCCTTTTGCAAGAGACAGGGTGGTGGAGGTGTTCTTTTGGATGGTGGGAGTATATTTTGAACCCCAGTACTCTATTGGCAGAATCATTATGACAAAAGTGGGTATCCTCCTAACTATTTTGGATGATATCTATGATGCATATGGTACATTTGAAGAACTCAAGATCTTCACTCAAGCAATTGACAGGTGGAATGTCAAATGCATAGATGAACTCCCAGACTATTTGAAAATATTCTATCATGAACTTTTGAATCTTTTCGATGAAATTGACAAAGTGATGTCAAAGGAAGGAAGATCATATCGAACTTCATATGCAATAGAAGCTATGAAAGTTCAGGCTCGATCATTTTTCAACGAGGCCCAATGGTTGCAAGAAGGACGCACTCCGAGCATGGAAGAATATATGAGTGTTGCTACAGTTTCTGTTACTTACCCTTTTATAACAACCATTTCATTACTTGGCATGGGAGATATTGTAACGAAGGATGCATTTGAGTGGTTGTTGGATGACCCGAAAATTGTTAGCGCTTCCAATATCATTTTTAGGCTCATGGATGACATTGTTTCCACCAAGTTTGAGAAAGAGAGAGGGCATGCACCTTCTAGCATTGATTGCTATATGAAGCAATATGGTGTTTCAGAGCAAGAGACGATTGACGTTTTCGGGAAACAAATTGCGAAATCATGGAAGGATATAAATGAGGAGTTTCTTAAACCAACCGCTATGCCAATGCCGGTTCTTATGCGTGTTCTCAATTTAACAAGAGTAGTGGATCTTCTTTACAAGGGAGAAGATGGATTCACACGAGTTGGGAAGATGACCAAAGATAGTGTTGCTGCAGTTATTATCGACTCAGTGCCACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

558

Amino Acids

64.8

Weight (kDa)

5.64

Isoelectric Point (pI)

42.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 33 - 205 3.7e-54 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 262 - 501 1.2e-97 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 302 - 501 2.4e-57 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1250
AccII CGCG 1 cut(s) 261
AciI CCGC 1 cut(s) 1530
AclWI GGATC 2 cut(s) 318, 1590
AcoI YGGCCR 1 cut(s) 328
AcsI RAATTY 2 cut(s) 79, 384
AcuI CTGAAG 1 cut(s) 627
AdeI CACNNNGTG 1 cut(s) 695
AfaI GTAC 5 cut(s) 39, 317, 554, 878, 955
AfeI AGCGCT 1 cut(s) 1336
AhlI ACTAGT 1 cut(s) 17
AluBI AGCT 5 cut(s) 429, 576, 611, 760, 1133
AluI AGCT 5 cut(s) 429, 576, 611, 760, 1133
Alw21I GWGCWC 2 cut(s) 561, 762
Alw26I GTCTC 3 cut(s) 613, 816, 1451
AlwI GGATC 2 cut(s) 318, 1590
AlwNI CAGNNNCTG 1 cut(s) 1232
Aor51HI AGCGCT 1 cut(s) 1336
AoxI GGCC 2 cut(s) 328, 1167
ApeKI GCWGC 1 cut(s) 1646
ApoI RAATTY 2 cut(s) 79, 384
Asp700I GAANNNNTTC 3 cut(s) 63, 1040, 1064
AspLEI GCGC 1 cut(s) 1337
AspS9I GGNCC 1 cut(s) 1168
AsuHPI GGTGA 2 cut(s) 203, 526
AsuII TTCGAA 1 cut(s) 628
BalI TGGCCA 1 cut(s) 330
BanII GRGCYC 1 cut(s) 762
BarI GAAGNNNNNNTAC 2 cut(s) 1569, 1601
BauI CACGAG 1 cut(s) 1614
Bbv12I GWGCWC 2 cut(s) 561, 762
BbvI GCAGC 1 cut(s) 1633
BccI CCATC 4 cut(s) 320, 339, 844, 1598
BciVI GTATCC 1 cut(s) 923
BcoDI GTCTC 3 cut(s) 613, 816, 1451
BcuI ACTAGT 1 cut(s) 17
BfaI CTAG 4 cut(s) 18, 774, 791, 1413
BfmI CTRYAG 2 cut(s) 1224, 1647
BfoI RGCGCY 1 cut(s) 1338
BfuI GTATCC 1 cut(s) 923
BglII AGATCT 1 cut(s) 972
BisI GCNGC 1 cut(s) 1647
BlpI GCTNAGC 1 cut(s) 467
BlsI GCNGC 1 cut(s) 1648
BmcAI AGTACT 1 cut(s) 878
BmgT120I GGNCC 1 cut(s) 1168
BmrI ACTGGG 1 cut(s) 868
BmsI GCATC 4 cut(s) 211, 442, 934, 1285
BmuI ACTGGG 1 cut(s) 868
Bpu1102I GCTNAGC 1 cut(s) 467
Bpu14I TTCGAA 1 cut(s) 628
BpuEI CTTGAG 4 cut(s) 194, 533, 953, 966
BsaAI YACGTR 1 cut(s) 556
BsaBI GATNNNNATC 3 cut(s) 309, 330, 729
BsaI GGTCTC 1 cut(s) 613
BsaWI WCCGGW 1 cut(s) 458
BsaXI ACNNNNNCTCC 2 cut(s) 1269, 1299
Bse118I RCCGGY 1 cut(s) 1543
Bse1I ACTGG 3 cut(s) 35, 584, 874
Bse3DI GCAATG 2 cut(s) 275, 574
Bse8I GATNNNNATC 3 cut(s) 309, 330, 729
BseGI GGATG 5 cut(s) 855, 937, 1300, 1321, 1369
BseJI GATNNNNATC 3 cut(s) 309, 330, 729
BseMI GCAATG 2 cut(s) 275, 574
BseMII CTCAG 3 cut(s) 149, 356, 1677
BseNI ACTGG 3 cut(s) 35, 584, 874
BseRI GAGGAG 1 cut(s) 1526
BseXI GCAGC 1 cut(s) 1633
Bsh1236I CGCG 1 cut(s) 261
BshFI GGCC 2 cut(s) 330, 1169
BsiHKAI GWGCWC 2 cut(s) 561, 762
BsiSI CCGG 3 cut(s) 70, 459, 1544
BsmAI GTCTC 3 cut(s) 613, 816, 1451
BsmBI CGTCTC 1 cut(s) 1451
BsmI GAATGC 1 cut(s) 443
BsnI GGCC 2 cut(s) 330, 1169
Bso31I GGTCTC 1 cut(s) 613
Bsp119I TTCGAA 1 cut(s) 628
Bsp1286I GDGCHC 2 cut(s) 561, 762
Bsp143I GATC 7 cut(s) 103, 304, 310, 972, 1103, 1151, 1582
Bsp1720I GCTNAGC 1 cut(s) 467
BspACI CCGC 1 cut(s) 1530
BspANI GGCC 2 cut(s) 330, 1169
BspCNI CTCAG 3 cut(s) 148, 357, 1676
BspFNI CGCG 1 cut(s) 261
BspHI TCATGA 1 cut(s) 1048
BspMAI CTGCAG 1 cut(s) 1651
BspPI GGATC 2 cut(s) 318, 1590
BspT104I TTCGAA 1 cut(s) 628
BspTNI GGTCTC 1 cut(s) 613
BsrDI GCAATG 2 cut(s) 275, 574
BsrFI RCCGGY 1 cut(s) 1543
BsrI ACTGG 3 cut(s) 35, 584, 874
BssAI RCCGGY 1 cut(s) 1543
BssMI GATC 7 cut(s) 103, 304, 310, 972, 1103, 1151, 1582
BssSI CACGAG 1 cut(s) 1614
Bst2BI CACGAG 1 cut(s) 1614
Bst4CI ACNGT 5 cut(s) 160, 320, 695, 1228, 1674
Bst6I CTCTTC 2 cut(s) 513, 630
BstAPI GCANNNNNTGC 1 cut(s) 1422
BstBAI YACGTR 1 cut(s) 556
BstBI TTCGAA 1 cut(s) 628
BstC8I GCNNGC 1 cut(s) 1404
BstDEI CTNAG 5 cut(s) 135, 365, 467, 491, 1663
BstF5I GGATG 5 cut(s) 855, 937, 1300, 1321, 1369
BstFNI CGCG 1 cut(s) 261
BstH2I RGCGCY 1 cut(s) 1338
BstHHI GCGC 1 cut(s) 1337
BstKTI GATC 7 cut(s) 106, 307, 313, 975, 1106, 1154, 1585
BstMAI GTCTC 3 cut(s) 613, 816, 1451
BstMBI GATC 7 cut(s) 103, 304, 310, 972, 1103, 1151, 1582
BstMWI GCNNNNNNNGC 5 cut(s) 367, 573, 1130, 1197, 1422
BstNSI RCATGY 1 cut(s) 1406
BstSFI CTRYAG 2 cut(s) 1224, 1647
BstUI CGCG 1 cut(s) 261
BstV1I GCAGC 1 cut(s) 1633
BstX2I RGATCY 3 cut(s) 310, 972, 1582
BstXI CCANNNNNNTGG 2 cut(s) 96, 236
BstYI RGATCY 3 cut(s) 310, 972, 1582
BsuI GTATCC 1 cut(s) 923
BsuRI GGCC 2 cut(s) 330, 1169
BtsCI GGATG 5 cut(s) 855, 937, 1300, 1321, 1369
BtsIMutI CAGTG 3 cut(s) 700, 1670, 1671
Cac8I GCNNGC 1 cut(s) 1404
CaiI CAGNNNCTG 1 cut(s) 1232
CciI TCATGA 1 cut(s) 1048
CfoI GCGC 1 cut(s) 1337
Cfr10I RCCGGY 1 cut(s) 1543
Cfr13I GGNCC 1 cut(s) 1168
CseI GACGC 1 cut(s) 1197
Csp6I GTAC 5 cut(s) 38, 316, 553, 877, 954
CspCI CAANNNNNGTGG 6 cut(s) 570, 605, 1366, 1401, 1560, 1595
CviAII CATG 8 cut(s) 290, 374, 1049, 1201, 1273, 1360, 1403, 1494
CviQI GTAC 5 cut(s) 38, 316, 553, 877, 954
DdeI CTNAG 5 cut(s) 135, 365, 467, 491, 1663
DpnI GATC 7 cut(s) 105, 306, 312, 974, 1105, 1153, 1584
DpnII GATC 7 cut(s) 103, 304, 310, 972, 1103, 1151, 1582
DraI TTTAAA 1 cut(s) 412
DraIII CACNNNGTG 1 cut(s) 695
EaeI YGGCCR 1 cut(s) 328
Eam1104I CTCTTC 2 cut(s) 513, 630
EarI CTCTTC 2 cut(s) 513, 630
Ecl136II GAGCTC 1 cut(s) 760
Eco24I GRGCYC 1 cut(s) 762
Eco31I GGTCTC 1 cut(s) 613
Eco32I GATATC 2 cut(s) 380, 937
Eco47III AGCGCT 1 cut(s) 1336
Eco53kI GAGCTC 1 cut(s) 760
Eco57I CTGAAG 1 cut(s) 627
EcoICRI GAGCTC 1 cut(s) 760
EcoRV GATATC 2 cut(s) 380, 937
EcoT22I ATGCAT 3 cut(s) 949, 1013, 1300
EcoT38I GRGCYC 1 cut(s) 762
Esp3I CGTCTC 1 cut(s) 1451
FaeI CATG 8 cut(s) 293, 377, 1052, 1204, 1276, 1363, 1406, 1497
FalI AAGNNNNNCTT 4 cut(s) 498, 530, 510, 542
FatI CATG 8 cut(s) 289, 373, 1048, 1200, 1272, 1359, 1402, 1493
FauNDI CATATG 2 cut(s) 949, 1120
Fnu4HI GCNGC 1 cut(s) 1647
FokI GGATG 5 cut(s) 862, 944, 1307, 1328, 1376
FriOI GRGCYC 1 cut(s) 762
Fsp4HI GCNGC 1 cut(s) 1647
FspBI CTAG 4 cut(s) 18, 774, 791, 1413
GlaI GCGC 1 cut(s) 1336
GluI GCNGC 1 cut(s) 1647
HaeII RGCGCY 1 cut(s) 1338
HaeIII GGCC 2 cut(s) 330, 1169
HapII CCGG 3 cut(s) 70, 459, 1544
HgaI GACGC 1 cut(s) 1197
HhaI GCGC 1 cut(s) 1337
Hin1II CATG 8 cut(s) 293, 377, 1052, 1204, 1276, 1363, 1406, 1497
Hin6I GCGC 1 cut(s) 1335
HinP1I GCGC 1 cut(s) 1335
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HindIII AAGCTT 1 cut(s) 609
HinfI GANTC 9 cut(s) 122, 207, 272, 416, 698, 891, 1060, 1608, 1661
HpaII CCGG 3 cut(s) 70, 459, 1544
HphI GGTGA 2 cut(s) 203, 526
Hpy166II GTNNAC 2 cut(s) 145, 534
Hpy188I TCNGA 5 cut(s) 366, 451, 766, 1197, 1450
Hpy188III TCNNGA 5 cut(s) 302, 308, 970, 1049, 1474
Hpy8I GTNNAC 2 cut(s) 145, 534
HpyAV CCTTC 7 cut(s) 182, 586, 1091, 1178, 1285, 1419, 1492
HpyCH4III ACNGT 5 cut(s) 160, 320, 695, 1228, 1674
HpyCH4IV ACGT 2 cut(s) 555, 1467
HpyF10VI GCNNNNNNNGC 5 cut(s) 367, 573, 1130, 1197, 1422
HpyF3I CTNAG 5 cut(s) 135, 365, 467, 491, 1663
HpySE526I ACGT 2 cut(s) 555, 1467
Hsp92II CATG 8 cut(s) 293, 377, 1052, 1204, 1276, 1363, 1406, 1497
HspAI GCGC 1 cut(s) 1335
Kzo9I GATC 7 cut(s) 103, 304, 310, 972, 1103, 1151, 1582
LmnI GCTCC 1 cut(s) 757
Lsp1109I GCAGC 1 cut(s) 1633
LweI GCATC 4 cut(s) 211, 442, 934, 1285
MaeI CTAG 4 cut(s) 18, 774, 791, 1413
MaeII ACGT 2 cut(s) 555, 1467
MaeIII GTNAC 2 cut(s) 1234, 1285
MalI GATC 7 cut(s) 105, 306, 312, 974, 1105, 1153, 1584
MboI GATC 7 cut(s) 103, 304, 310, 972, 1103, 1151, 1582
MfeI CAATTG 1 cut(s) 987
MflI RGATCY 3 cut(s) 310, 972, 1582
MhlI GDGCHC 2 cut(s) 561, 762
MlsI TGGCCA 1 cut(s) 330
MluNI TGGCCA 1 cut(s) 330
MlyI GAGTC 2 cut(s) 201, 1655
MmeI TCCRAC 3 cut(s) 253, 702, 1293
Mox20I TGGCCA 1 cut(s) 330
Mph1103I ATGCAT 3 cut(s) 949, 1013, 1300
MroXI GAANNNNTTC 3 cut(s) 63, 1040, 1064
MscI TGGCCA 1 cut(s) 330
MseI TTAA 6 cut(s) 215, 411, 443, 498, 1521, 1568
MslI CAYNNNNRTG 2 cut(s) 288, 1303
Msp20I TGGCCA 1 cut(s) 330
MspI CCGG 3 cut(s) 70, 459, 1544
MunI CAATTG 1 cut(s) 987
Mva1269I GAATGC 1 cut(s) 443
MvnI CGCG 1 cut(s) 261
MwoI GCNNNNNNNGC 5 cut(s) 367, 573, 1130, 1197, 1422
NdeI CATATG 2 cut(s) 949, 1120
NdeII GATC 7 cut(s) 103, 304, 310, 972, 1103, 1151, 1582
NlaIII CATG 8 cut(s) 293, 377, 1052, 1204, 1276, 1363, 1406, 1497
NsiI ATGCAT 3 cut(s) 949, 1013, 1300
NspI RCATGY 1 cut(s) 1406
NspV TTCGAA 1 cut(s) 628
PaeI GCATGC 1 cut(s) 1406
PagI TCATGA 1 cut(s) 1048
PctI GAATGC 1 cut(s) 443
PdmI GAANNNNTTC 3 cut(s) 63, 1040, 1064
PfeI GAWTC 7 cut(s) 122, 272, 416, 698, 891, 1060, 1608
PkrI GCNGC 1 cut(s) 1648
PleI GAGTC 2 cut(s) 201, 1655
PpsI GAGTC 2 cut(s) 201, 1655
Ppu21I YACGTR 1 cut(s) 556
PsiI TTATAA 1 cut(s) 1250
Psp124BI GAGCTC 1 cut(s) 762
PspPI GGNCC 1 cut(s) 1168
PstI CTGCAG 1 cut(s) 1651
PstNI CAGNNNCTG 1 cut(s) 1232
PsuI RGATCY 3 cut(s) 310, 972, 1582
RsaI GTAC 5 cut(s) 39, 317, 554, 878, 955
RsaNI GTAC 5 cut(s) 38, 316, 553, 877, 954
RseI CAYNNNNRTG 2 cut(s) 288, 1303
SacI GAGCTC 1 cut(s) 762
SaqAI TTAA 6 cut(s) 215, 411, 443, 498, 1521, 1568
SatI GCNGC 1 cut(s) 1647
Sau3AI GATC 7 cut(s) 103, 304, 310, 972, 1103, 1151, 1582
Sau96I GGNCC 1 cut(s) 1168
ScaI AGTACT 1 cut(s) 878
SchI GAGTC 2 cut(s) 201, 1655
SduI GDGCHC 2 cut(s) 561, 762
SfaNI GCATC 4 cut(s) 211, 442, 934, 1285
SfcI CTRYAG 2 cut(s) 1224, 1647
SfuI TTCGAA 1 cut(s) 628
SmiMI CAYNNNNRTG 2 cut(s) 288, 1303
SmlI CTYRAG 4 cut(s) 209, 548, 968, 981
SmoI CTYRAG 4 cut(s) 209, 548, 968, 981
SpeI ACTAGT 1 cut(s) 17
SphI GCATGC 1 cut(s) 1406
SsiI CCGC 1 cut(s) 1530
SspI AATATT 1 cut(s) 1041
SspMI CTAG 4 cut(s) 18, 774, 791, 1413
SstI GAGCTC 1 cut(s) 762
TaaI ACNGT 5 cut(s) 160, 320, 695, 1228, 1674
TaiI ACGT 2 cut(s) 558, 1470
TaqI TCGA 6 cut(s) 205, 628, 1068, 1111, 1150, 1659
TatI WGTACW 1 cut(s) 876
TfiI GAWTC 7 cut(s) 122, 272, 416, 698, 891, 1060, 1608
Tru1I TTAA 6 cut(s) 215, 411, 443, 498, 1521, 1568
Tru9I TTAA 6 cut(s) 215, 411, 443, 498, 1521, 1568
TscAI CASTG 3 cut(s) 700, 1671, 1677
TseI GCWGC 1 cut(s) 1646
TspRI CASTG 3 cut(s) 700, 1671, 1677
XapI RAATTY 2 cut(s) 79, 384
XceI RCATGY 1 cut(s) 1406
XcmI CCANNNNNNNNNTGG 1 cut(s) 881
XmnI GAANNNNTTC 3 cut(s) 63, 1040, 1064
XspI CTAG 4 cut(s) 18, 774, 791, 1413
ZrmI AGTACT 1 cut(s) 878
Zsp2I ATGCAT 3 cut(s) 949, 1013, 1300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.