Rw5G027380

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000053
Physical Location & Seq
Reverse (-)
40389033 .. 40392057
3025 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G027380.1

Sequence Viewer

Length: 1611 bp
ATGTCTATGGCTACAGATCCTTCCCCATCAAGTCAAGTACCACAAATTGCTAAGCCTGAAATTGTTCGCCAGACGGCGAATTTTCAACCAAGCATTTGGGGAGATCAGTTCATCAACTATGATTCTCAAGACATTAAGACTGAGGCCCTTTGGCAACAAGAAGTCGAGGAACTGAAAGTAGTAGTGAAGAGAGAAGTATTTACAAATGGGAAAGGTGATTTTTCCCATCAACTCAAGTTGATCGATGCAATCCAACGCCTTGGCGTCGCATACCATTTTGAACAAGAAATAGAATATGCAATGCAACACATCCATGCTACATATCATGATCAGGATCACTACCGTGATAATGGCGATCTTTACACTACTGCGCTATGTTTTCGATTGCTAAGGCAACATGGATATGATATCTCATCTGATATATTCAACAAGTTCAAAGATGCAAATGGTAACTTCAAAGAAGGCTTAGTTGTCGATGGGTCAGCTATGCTGAGCCTCTACGAAGCTGCACATCTTAAGGTTCATGGAGAAGATATACTAGAAGAGGCTCTTGTTTTCACCACTACTCACCTCGAGTCCGCAAAATCCAATGCGAGCTATGGAGTACAAATGACTGATCAAATCACTCAAGCCTTGGCGAGACCTCTGCGAAAAAGTCTAGAGAGGATATGTGCCAAGCGTCACATGTCAATTTACCAACATGAAGATGAAGCCTCACTCATCAACGACAATGAAGCTTTATTGAGACTTGCAAAGTTGGATTTCAATCTTGTTCAGTCTTTACACAAAAAAGAGTTGTGTGAAATTAGTAGGTGGTGGAAAGAACTAGACTTTGAAAGGAAATTGCCTTTTGCAAGAGACAGGAAGGTGGAGTTGTTCTTTTGGATAGTGGGAGTATATTTTGAACCCCAGTACTCTATTGGCAGAACCATTATGACAAAAGTGTCTATCCTCCTAACTGTTTTGGATGATATATATGACGCATATGGTACATTTGAAGAACTCGTGATCTTTACTGAAGCAATTGACAGTTCATTGGCTTATAATATATATGTGTATATTTCTCAGATGAAAAATCAAGCTCGATCATATTTCAACGAGGCCCAATGGTTGCACGAAGGACGCACTCCAAGCATTGAGGAGTATATGAGGGTTGCAACAGTTTCTATTAGTTACACTTTTCTAACAACCATTTCATTACTCGGCATGGGAGATATTGTAACAAAGGATGCATTTGAGTGGTTGTTGAATGACCCGAAAATTGTTAGAGCTTCCAATATCATCTTTAGGCTTATGGATGACATTGTTTCCACCAAGTTTGAGAAAGAGAGAGAGCATGCACCTTCTAGCATTGATTGCTATATGAAGCAATATAGTGTTTCAGAGCAAGAGACAATTGATATTTTCAACAAACAAATTGTGGAATCATGGAAGGATATAAACGAGGAGTTTCGTAAACCAACCGCCGTGCCAATATCGGTTCTTATGCGCGTTCTCAATTTAACAAGAGTTGCGGATCTCCTTTATAAGGGAGAAGATGGATTCACACGGGTTGGGAAGATGACCAAAGCTAGTGTTGCTGCAGTTATTATCGATCAAGTGCCACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

536

Amino Acids

61.87

Weight (kDa)

5.28

Isoelectric Point (pI)

39.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 32 - 212 1.4e-57 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 274 - 343 8.3e-28 Terpene synthase family, metal binding domain
Terpene_synth_C PF03936 349 - 479 3.5e-44 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 353 - 479 8.3e-37 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1044, 1527
AasI GACNNNNNNGTC 1 cut(s) 943
AccII CGCG 1 cut(s) 1491
AciI CCGC 3 cut(s) 579, 1464, 1514
AclWI GGATC 3 cut(s) 11, 342, 1524
AcsI RAATTY 1 cut(s) 79
AcuI CTGAAG 1 cut(s) 1038
AcyI GRCGYC 1 cut(s) 264
AfaI GTAC 4 cut(s) 39, 606, 914, 991
AfiI CCNNNNNNNGG 1 cut(s) 1528
AflII CTTAAG 1 cut(s) 515
AflIII ACRYGT 1 cut(s) 684
AleI CACNNNNGTG 1 cut(s) 342
AluBI AGCT 7 cut(s) 485, 506, 597, 737, 1082, 1271, 1571
AluI AGCT 7 cut(s) 485, 506, 597, 737, 1082, 1271, 1571
Alw26I GTCTC 4 cut(s) 634, 739, 852, 1385
AlwI GGATC 3 cut(s) 11, 342, 1524
Ama87I CYCGRG 1 cut(s) 572
AoxI GGCC 2 cut(s) 144, 1101
ApeKI GCWGC 2 cut(s) 506, 1580
ApoI RAATTY 1 cut(s) 79
Asp700I GAANNNNTTC 1 cut(s) 63
AspLEI GCGC 2 cut(s) 373, 1491
AspS9I GGNCC 2 cut(s) 145, 1102
AsuHPI GGTGA 3 cut(s) 227, 550, 560
AvaI CYCGRG 1 cut(s) 572
BauI CACGAG 1 cut(s) 1004
BbvI GCAGC 2 cut(s) 493, 1567
BccI CCATC 4 cut(s) 34, 234, 470, 1532
BceAI ACGGC 2 cut(s) 90, 1451
BcgI CGANNNNNNTGC 2 cut(s) 628, 662
BclI TGATCA 2 cut(s) 328, 616
BcoDI GTCTC 4 cut(s) 634, 739, 852, 1385
BfaI CTAG 5 cut(s) 539, 659, 827, 1347, 1572
BfmI CTRYAG 2 cut(s) 12, 1581
BfrI CTTAAG 1 cut(s) 515
BisI GCNGC 2 cut(s) 507, 1581
BlpI GCTNAGC 2 cut(s) 51, 491
BlsI GCNGC 2 cut(s) 508, 1582
BmcAI AGTACT 1 cut(s) 914
BmeT110I CYCGRG 1 cut(s) 572
BmgT120I GGNCC 2 cut(s) 145, 1102
BmrI ACTGGG 1 cut(s) 904
BmsI GCATC 3 cut(s) 235, 430, 1219
BmuI ACTGGG 1 cut(s) 904
Bpu10I CCTNAGC 1 cut(s) 389
Bpu1102I GCTNAGC 2 cut(s) 51, 491
BpuEI CTTGAG 3 cut(s) 111, 218, 612
Bsa29I ATCGAT 2 cut(s) 243, 1593
BsaBI GATNNNNATC 2 cut(s) 333, 765
BsaHI GRCGYC 1 cut(s) 264
BsaI GGTCTC 1 cut(s) 634
BsaJI CCNNGG 2 cut(s) 259, 633
BsaXI ACNNNNNCTCC 2 cut(s) 1203, 1233
Bsc4I CCNNNNNNNGG 1 cut(s) 1528
Bse1I ACTGG 1 cut(s) 910
Bse3DI GCAATG 1 cut(s) 306
Bse8I GATNNNNATC 2 cut(s) 333, 765
BseCI ATCGAT 2 cut(s) 243, 1593
BseDI CCNNGG 2 cut(s) 259, 633
BseGI GGATG 4 cut(s) 309, 973, 1234, 1303
BseJI GATNNNNATC 2 cut(s) 333, 765
BseLI CCNNNNNNNGG 1 cut(s) 1528
BseMI GCAATG 1 cut(s) 306
BseMII CTCAG 3 cut(s) 132, 482, 1079
BseNI ACTGG 1 cut(s) 910
BseRI GAGGAG 2 cut(s) 1154, 1460
BseXI GCAGC 2 cut(s) 493, 1567
BsgI GTGCAG 1 cut(s) 492
Bsh1236I CGCG 1 cut(s) 1491
BshFI GGCC 2 cut(s) 146, 1103
BshVI ATCGAT 2 cut(s) 243, 1593
BsiHKCI CYCGRG 1 cut(s) 572
BslI CCNNNNNNNGG 1 cut(s) 1528
BsmAI GTCTC 4 cut(s) 634, 739, 852, 1385
BsnI GGCC 2 cut(s) 146, 1103
Bso31I GGTCTC 1 cut(s) 634
BsoBI CYCGRG 1 cut(s) 572
Bsp1720I GCTNAGC 2 cut(s) 51, 491
BspACI CCGC 3 cut(s) 579, 1464, 1514
BspANI GGCC 2 cut(s) 146, 1103
BspCNI CTCAG 3 cut(s) 133, 483, 1078
BspDI ATCGAT 2 cut(s) 243, 1593
BspFNI CGCG 1 cut(s) 1491
BspHI TCATGA 1 cut(s) 325
BspMAI CTGCAG 1 cut(s) 1585
BspPI GGATC 3 cut(s) 11, 342, 1524
BspTI CTTAAG 1 cut(s) 515
BspTNI GGTCTC 1 cut(s) 634
BsrDI GCAATG 1 cut(s) 306
BsrI ACTGG 1 cut(s) 910
BssECI CCNNGG 2 cut(s) 259, 633
BssNI GRCGYC 1 cut(s) 264
BssSI CACGAG 1 cut(s) 1004
BssT1I CCWWGG 2 cut(s) 259, 633
Bst2BI CACGAG 1 cut(s) 1004
Bst4CI ACNGT 5 cut(s) 344, 961, 1031, 1162, 1608
Bst6I CTCTTC 2 cut(s) 182, 537
BstACI GRCGYC 1 cut(s) 264
BstAFI CTTAAG 1 cut(s) 515
BstAPI GCANNNNNTGC 1 cut(s) 1356
BstC8I GCNNGC 2 cut(s) 595, 1338
BstDEI CTNAG 6 cut(s) 51, 141, 389, 466, 491, 1065
BstENI CCTNNNNNAGG 1 cut(s) 1526
BstF5I GGATG 4 cut(s) 309, 973, 1234, 1303
BstFNI CGCG 1 cut(s) 1491
BstHHI GCGC 2 cut(s) 373, 1491
BstMAI GTCTC 4 cut(s) 634, 739, 852, 1385
BstMWI GCNNNNNNNGC 3 cut(s) 1131, 1356, 1577
BstNSI RCATGY 2 cut(s) 688, 1340
BstSFI CTRYAG 2 cut(s) 12, 1581
BstUI CGCG 1 cut(s) 1491
BstV1I GCAGC 2 cut(s) 493, 1567
BstX2I RGATCY 2 cut(s) 16, 1516
BstXI CCANNNNNNTGG 2 cut(s) 96, 260
BstYI RGATCY 2 cut(s) 16, 1516
Bsu15I ATCGAT 2 cut(s) 243, 1593
BsuRI GGCC 2 cut(s) 146, 1103
BsuTUI ATCGAT 2 cut(s) 243, 1593
BtsCI GGATG 4 cut(s) 309, 973, 1234, 1303
BtsIMutI CAGTG 1 cut(s) 1604
Cac8I GCNNGC 2 cut(s) 595, 1338
CciI TCATGA 1 cut(s) 325
CfoI GCGC 2 cut(s) 373, 1491
Cfr13I GGNCC 2 cut(s) 145, 1102
ClaI ATCGAT 2 cut(s) 243, 1593
CseI GACGC 4 cut(s) 253, 668, 989, 1131
Csp6I GTAC 4 cut(s) 38, 605, 913, 990
CspCI CAANNNNNGTGG 2 cut(s) 1300, 1335
CviAII CATG 9 cut(s) 314, 326, 398, 524, 685, 701, 1207, 1337, 1428
CviQI GTAC 4 cut(s) 38, 605, 913, 990
DdeI CTNAG 6 cut(s) 51, 141, 389, 466, 491, 1065
DrdI GACNNNNNNGTC 1 cut(s) 943
DseDI GACNNNNNNGTC 1 cut(s) 943
Eam1104I CTCTTC 2 cut(s) 182, 537
EarI CTCTTC 2 cut(s) 182, 537
Eco130I CCWWGG 2 cut(s) 259, 633
Eco31I GGTCTC 1 cut(s) 634
Eco32I GATATC 1 cut(s) 409
Eco57I CTGAAG 1 cut(s) 1038
Eco88I CYCGRG 1 cut(s) 572
EcoNI CCTNNNNNAGG 1 cut(s) 1526
EcoO109I RGGNCCY 1 cut(s) 145
EcoRV GATATC 1 cut(s) 409
EcoT14I CCWWGG 2 cut(s) 259, 633
EcoT22I ATGCAT 1 cut(s) 1234
ErhI CCWWGG 2 cut(s) 259, 633
FaeI CATG 9 cut(s) 317, 329, 401, 527, 688, 704, 1210, 1340, 1431
FalI AAGNNNNNCTT 2 cut(s) 534, 566
FatI CATG 9 cut(s) 313, 325, 397, 523, 684, 700, 1206, 1336, 1427
FauNDI CATATG 1 cut(s) 985
FbaI TGATCA 2 cut(s) 328, 616
Fnu4HI GCNGC 2 cut(s) 507, 1581
FokI GGATG 4 cut(s) 296, 980, 1241, 1310
Fsp4HI GCNGC 2 cut(s) 507, 1581
FspBI CTAG 5 cut(s) 539, 659, 827, 1347, 1572
GlaI GCGC 2 cut(s) 372, 1490
GluI GCNGC 2 cut(s) 507, 1581
HaeIII GGCC 2 cut(s) 146, 1103
HgaI GACGC 4 cut(s) 253, 668, 989, 1131
HhaI GCGC 2 cut(s) 373, 1491
Hin1I GRCGYC 1 cut(s) 264
Hin1II CATG 9 cut(s) 317, 329, 401, 527, 688, 704, 1210, 1340, 1431
Hin6I GCGC 2 cut(s) 371, 1489
HinP1I GCGC 2 cut(s) 371, 1489
HindIII AAGCTT 1 cut(s) 735
HinfI GANTC 4 cut(s) 122, 575, 1424, 1542
HphI GGTGA 3 cut(s) 227, 550, 560
Hpy166II GTNNAC 1 cut(s) 1457
Hpy188I TCNGA 3 cut(s) 418, 1068, 1384
Hpy188III TCNNGA 5 cut(s) 128, 326, 332, 659, 1006
Hpy8I GTNNAC 1 cut(s) 1457
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 6 cut(s) 30, 455, 859, 1112, 1353, 1426
HpyCH4III ACNGT 5 cut(s) 344, 961, 1031, 1162, 1608
HpyF10VI GCNNNNNNNGC 3 cut(s) 1131, 1356, 1577
HpyF3I CTNAG 6 cut(s) 51, 141, 389, 466, 491, 1065
Hsp92I GRCGYC 1 cut(s) 264
Hsp92II CATG 9 cut(s) 317, 329, 401, 527, 688, 704, 1210, 1340, 1431
HspAI GCGC 2 cut(s) 371, 1489
Ksp22I TGATCA 2 cut(s) 328, 616
LpnPI CCDG 5 cut(s) 69, 83, 317, 847, 923
Lsp1109I GCAGC 2 cut(s) 493, 1567
LweI GCATC 3 cut(s) 235, 430, 1219
MaeI CTAG 5 cut(s) 539, 659, 827, 1347, 1572
MaeIII GTNAC 4 cut(s) 449, 680, 1172, 1219
MboII GAAGA 7 cut(s) 199, 542, 554, 716, 1010, 1547, 1570
MfeI CAATTG 2 cut(s) 1023, 1395
MflI RGATCY 2 cut(s) 16, 1516
MlyI GAGTC 1 cut(s) 584
MmeI TCCRAC 2 cut(s) 277, 738
Mph1103I ATGCAT 1 cut(s) 1234
MroXI GAANNNNTTC 1 cut(s) 63
MseI TTAA 3 cut(s) 135, 516, 1502
MslI CAYNNNNRTG 5 cut(s) 312, 342, 402, 705, 1237
MspCI CTTAAG 1 cut(s) 515
MunI CAATTG 2 cut(s) 1023, 1395
MvnI CGCG 1 cut(s) 1491
MwoI GCNNNNNNNGC 3 cut(s) 1131, 1356, 1577
NdeI CATATG 1 cut(s) 985
NlaIII CATG 9 cut(s) 317, 329, 401, 527, 688, 704, 1210, 1340, 1431
NmeAIII GCCGAG 1 cut(s) 1182
NmuCI GTSAC 1 cut(s) 680
NsiI ATGCAT 1 cut(s) 1234
NspI RCATGY 2 cut(s) 688, 1340
OliI CACNNNNGTG 1 cut(s) 342
PaeI GCATGC 1 cut(s) 1340
PaeR7I CTCGAG 1 cut(s) 572
PagI TCATGA 1 cut(s) 325
PciI ACATGT 1 cut(s) 684
PdmI GAANNNNTTC 1 cut(s) 63
PfeI GAWTC 3 cut(s) 122, 1424, 1542
PkrI GCNGC 2 cut(s) 508, 1582
PleI GAGTC 1 cut(s) 583
PpsI GAGTC 1 cut(s) 583
PscI ACATGT 1 cut(s) 684
PsiI TTATAA 2 cut(s) 1044, 1527
PspPI GGNCC 2 cut(s) 145, 1102
PspXI VCTCGAGB 1 cut(s) 572
PstI CTGCAG 1 cut(s) 1585
PsuI RGATCY 2 cut(s) 16, 1516
RsaI GTAC 4 cut(s) 39, 606, 914, 991
RsaNI GTAC 4 cut(s) 38, 605, 913, 990
RseI CAYNNNNRTG 5 cut(s) 312, 342, 402, 705, 1237
SaqAI TTAA 3 cut(s) 135, 516, 1502
SatI GCNGC 2 cut(s) 507, 1581
Sau96I GGNCC 2 cut(s) 145, 1102
ScaI AGTACT 1 cut(s) 914
SchI GAGTC 1 cut(s) 584
SfaNI GCATC 3 cut(s) 235, 430, 1219
SfcI CTRYAG 2 cut(s) 12, 1581
Sfr274I CTCGAG 1 cut(s) 572
SlaI CTCGAG 1 cut(s) 572
SmiMI CAYNNNNRTG 5 cut(s) 312, 342, 402, 705, 1237
SmlI CTYRAG 5 cut(s) 126, 233, 515, 572, 627
SmoI CTYRAG 5 cut(s) 126, 233, 515, 572, 627
SphI GCATGC 1 cut(s) 1340
SsiI CCGC 3 cut(s) 579, 1464, 1514
SspMI CTAG 5 cut(s) 539, 659, 827, 1347, 1572
StyI CCWWGG 2 cut(s) 259, 633
TaaI ACNGT 5 cut(s) 344, 961, 1031, 1162, 1608
TaqI TCGA 7 cut(s) 165, 243, 382, 474, 573, 1084, 1593
TatI WGTACW 2 cut(s) 604, 912
TfiI GAWTC 3 cut(s) 122, 1424, 1542
Tru1I TTAA 3 cut(s) 135, 516, 1502
Tru9I TTAA 3 cut(s) 135, 516, 1502
TscAI CASTG 1 cut(s) 1611
TseFI GTSAC 1 cut(s) 680
TseI GCWGC 2 cut(s) 506, 1580
Tsp45I GTSAC 1 cut(s) 680
TspDTI ATGAA 9 cut(s) 100, 512, 717, 723, 747, 1023, 1085, 1185, 1379
TspRI CASTG 1 cut(s) 1611
Vha464I CTTAAG 1 cut(s) 515
XagI CCTNNNNNAGG 1 cut(s) 1526
XapI RAATTY 1 cut(s) 79
XbaI TCTAGA 1 cut(s) 658
XceI RCATGY 2 cut(s) 688, 1340
XcmI CCANNNNNNNNNTGG 1 cut(s) 917
XhoI CTCGAG 1 cut(s) 572
XmnI GAANNNNTTC 1 cut(s) 63
XspI CTAG 5 cut(s) 539, 659, 827, 1347, 1572
ZrmI AGTACT 1 cut(s) 914
Zsp2I ATGCAT 1 cut(s) 1234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.