Prupe.4G194100_v2.0.a1

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000053
Physical Location & Seq
Forward (+)
11704060 .. 11707409
3350 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G194100.1

Sequence Viewer

Length: 1686 bp
ATGTCTATCCAAGTTTGTGCAGCAGCTCAATCACCAAATGCCAAGCCTGAAATTATTCGGCGGACAGCAAACTTCCCACCAAGCATTTGGGGTGATCGGTTTCTCAACTATGATTCCCAAGACGTTATAACAAATGCCCGGAACCAACAAGAAGTTGACAACCTGAAAGAAGTAGTGAGGAGGGAAGTATTCACAACTAGTGCAGGTGACTTTTCACATCAGCTCAAGTTGATTGATGCAATCCAGCGCCTTGGCGTGGCATACCATTTTGAAAGTGAAATTGAAGATGCATTGGAGCGTACGCATGCTACATTTCGTGACCATGCCTTTAGTGATGACGGAGATCTATACAACGTTGCTCTTGGTTTCCGGCTACTAAGACAACATGGATATAAGGTTTCATGTGATGTGTTCAACATGTTCAAAGACAAAAATGGCAGCTTCAAGGAATGCTTAATTGCCGATGTTCCGGGGATGCTAAGCCTTTATGAAGCCGGGCATCTAGGAGTACGGGGAGAAGAAATACTGGATGAAGCTCTTGCTTTCACCACCACTCACCTTGACTCTGCTGCAAAAGCTCATGTAAGCTACGAACATGCAGAACAAATCACTCAAGCCTTGGAGAGACCTTTGCAAAAAGATCTTGAGAGGTTATGTGCCAGGCGTTACATGTCAATCTACCAACATGAAGCTTCACACAATGAAGCTTTGTTGAAACTTGCAAAGTTAGATTTCAATCTTGTTCAGTGTTTGCACAAAAAGGAGCTCAGTGAGATTACTAGGTGGTGGAAAGAAGTGGACTTTGAAAAGACGCTGCCTTTTGCAAGAGACAGGATAGTGGAAATATACTTTTGGGTAATGGCAGTATATTTTGAACCTCACTACTCTGCCGCAAGAAATTTTTTAAACAAACTCCTTGCCCTGGGAACACTGATGGATGATATATACGATGCATTTGGTACCTTCGACGAACTTGAGATCTTTACAGAAGCAATTGGGAGGTGGGATGTCAATTGCATGGATGAACTACCAGACTATATGAAAATATTTTATCAGACACTTATGAATCTTTTCAATGAAATTGAGGAAGAGATGGTGAAGAAAGGGAATTCATACCGAGTTTACTATGCAAAAGAAGTTTGGAAAGCTACAGCCCGAGCTTACTTTGATGAGGCCAGATGGTTACACGAAGGATGCATCCCAAGCATGGAGGAGTATATGCATGTTGCCACAGCTTCTGTTGGTAACAGCCCACTTTCAACCATATCTTTACTTGGCATGGGAGATATTGTAACCAAGGAGGCATTTGAGTGGTTGCTCAATAACCCTAAAATCCTTAGAGCTTCGAATATCATTTTTAGGCTCATGGATGACGTTGCTGGGTACAAGTTTGACAAGGAGAGAGGGCATGTTGCTTCTAGTATTGATTGCTACATGAAGCAACATGGAGTCTCTGAGCAAGAGACACTTGATGTTTTCAACAAACAAGTTGTGGATTTGTGGAAGGATATAAACGAGGAGCTCCTTATAAAGCCAACTGTTGTGCCAAGGCCTGTCCTCATGCGCATTCTTAATTTAACAAGAGTTGTGGATCTGCTTTATAAAAGGGGAGATGGCTTCACACATGTTGGCAAACTTATGAAAGATATTGTTGCTTCACTTTTTCTAGATCCTGTTCCACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

562

Amino Acids

64.69

Weight (kDa)

5.33

Isoelectric Point (pI)

44.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 128, 1529, 1602
AarI CACCTGC 1 cut(s) 194
Acc16I TGCGCA 1 cut(s) 1565
Acc36I ACCTGC 1 cut(s) 194
Acc65I GGTACC 1 cut(s) 959
AccB1I GGYRCC 1 cut(s) 959
AciI CCGC 2 cut(s) 61, 891
AclI AACGTT 1 cut(s) 354
AclWI GGATC 2 cut(s) 1599, 1664
AcsI RAATTY 2 cut(s) 898, 1108
AfaI GTAC 4 cut(s) 301, 510, 961, 1385
AfiI CCNNNNNNNGG 3 cut(s) 256, 922, 1207
AflIII ACRYGT 3 cut(s) 417, 669, 1624
AhlI ACTAGT 1 cut(s) 197
AjnI CCWGG 2 cut(s) 659, 921
Alw21I GWGCWC 2 cut(s) 768, 1524
Alw26I GTCTC 4 cut(s) 619, 822, 1456, 1457
AlwI GGATC 2 cut(s) 1599, 1664
AlwNI CAGNNNCTG 1 cut(s) 1238
Ama87I CYCGRG 1 cut(s) 1155
AoxI GGCC 2 cut(s) 1173, 1550
ApeKI GCWGC 5 cut(s) 20, 23, 438, 569, 814
ApoI RAATTY 2 cut(s) 898, 1108
Asp700I GAANNNNTTC 2 cut(s) 54, 1070
Asp718I GGTACC 1 cut(s) 959
AspLEI GCGC 2 cut(s) 249, 1566
AsuC2I CCSGG 3 cut(s) 139, 471, 496
AsuHPI GGTGA 6 cut(s) 24, 104, 218, 538, 548, 1108
AsuII TTCGAA 1 cut(s) 1346
AvaI CYCGRG 1 cut(s) 1155
BanI GGYRCC 1 cut(s) 959
BanII GRGCYC 2 cut(s) 768, 1524
Bbv12I GWGCWC 2 cut(s) 768, 1524
BbvI GCAGC 5 cut(s) 32, 35, 450, 556, 801
BccI CCATC 4 cut(s) 928, 1087, 1173, 1607
BciT130I CCWGG 2 cut(s) 661, 923
BcnI CCSGG 3 cut(s) 139, 471, 496
BcoDI GTCTC 4 cut(s) 619, 822, 1456, 1457
BcuI ACTAGT 1 cut(s) 197
BfaI CTAG 5 cut(s) 198, 503, 780, 1419, 1667
BfmI CTRYAG 1 cut(s) 1149
BfoI RGCGCY 1 cut(s) 250
BfuAI ACCTGC 1 cut(s) 194
BglII AGATCT 3 cut(s) 343, 640, 978
BisI GCNGC 6 cut(s) 21, 24, 439, 570, 815, 891
BlpI GCTNAGC 1 cut(s) 479
BlsI GCNGC 6 cut(s) 22, 25, 440, 571, 816, 892
Bme1390I CCNGG 5 cut(s) 139, 471, 496, 661, 923
BmeT110I CYCGRG 1 cut(s) 1155
BmiI GGNNCC 2 cut(s) 143, 961
BmrFI CCNGG 5 cut(s) 139, 471, 496, 661, 923
BmsI GCATC 7 cut(s) 226, 277, 465, 508, 940, 1184, 1206
Bpu1102I GCTNAGC 1 cut(s) 479
Bpu14I TTCGAA 1 cut(s) 1346
BpuEI CTTGAG 4 cut(s) 209, 597, 665, 995
BpuMI CCSGG 3 cut(s) 139, 471, 496
BsaBI GATNNNNATC 1 cut(s) 735
BsaI GGTCTC 1 cut(s) 619
BsaJI CCNNGG 7 cut(s) 250, 470, 618, 921, 922, 1296, 1547
BsaXI ACNNNNNCTCC 2 cut(s) 1275, 1305
Bsc4I CCNNNNNNNGG 3 cut(s) 256, 922, 1207
Bse1I ACTGG 1 cut(s) 531
Bse8I GATNNNNATC 1 cut(s) 735
BseBI CCWGG 2 cut(s) 661, 923
BseDI CCNNGG 7 cut(s) 250, 470, 618, 921, 922, 1296, 1547
BseGI GGATG 8 cut(s) 480, 535, 943, 1012, 1027, 1197, 1199, 1375
BseJI GATNNNNATC 1 cut(s) 735
BseLI CCNNNNNNNGG 3 cut(s) 256, 922, 1207
BseMII CTCAG 2 cut(s) 781, 1446
BseNI ACTGG 1 cut(s) 531
BseRI GAGGAG 3 cut(s) 193, 1226, 1532
BseXI GCAGC 5 cut(s) 32, 35, 450, 556, 801
BseYI CCCAGC 1 cut(s) 1379
BsgI GTGCAG 2 cut(s) 39, 222
BshFI GGCC 2 cut(s) 1175, 1552
BshNI GGYRCC 1 cut(s) 959
BsiHKAI GWGCWC 2 cut(s) 768, 1524
BsiHKCI CYCGRG 1 cut(s) 1155
BsiSI CCGG 4 cut(s) 139, 370, 470, 495
BsiWI CGTACG 1 cut(s) 299
BslI CCNNNNNNNGG 3 cut(s) 256, 922, 1207
BsmAI GTCTC 4 cut(s) 619, 822, 1456, 1457
BsmI GAATGC 2 cut(s) 455, 1566
BsnI GGCC 2 cut(s) 1175, 1552
Bso31I GGTCTC 1 cut(s) 619
BsoBI CYCGRG 1 cut(s) 1155
Bsp119I TTCGAA 1 cut(s) 1346
Bsp1286I GDGCHC 2 cut(s) 768, 1524
Bsp143I GATC 6 cut(s) 94, 343, 640, 978, 1591, 1669
Bsp1720I GCTNAGC 1 cut(s) 479
BspACI CCGC 2 cut(s) 61, 891
BspANI GGCC 2 cut(s) 1175, 1552
BspCNI CTCAG 2 cut(s) 780, 1447
BspLI GGNNCC 2 cut(s) 143, 961
BspMI ACCTGC 1 cut(s) 194
BspPI GGATC 2 cut(s) 1599, 1664
BspT104I TTCGAA 1 cut(s) 1346
BspT107I GGYRCC 1 cut(s) 959
BspTNI GGTCTC 1 cut(s) 619
BsrI ACTGG 1 cut(s) 531
BssECI CCNNGG 7 cut(s) 250, 470, 618, 921, 922, 1296, 1547
BssMI GATC 6 cut(s) 94, 343, 640, 978, 1591, 1669
BssT1I CCWWGG 4 cut(s) 250, 618, 1296, 1547
Bst2UI CCWGG 2 cut(s) 661, 923
Bst4CI ACNGT 1 cut(s) 1540
Bst6I CTCTTC 1 cut(s) 1083
BstBI TTCGAA 1 cut(s) 1346
BstC8I GCNNGC 1 cut(s) 306
BstDEI CTNAG 5 cut(s) 377, 479, 767, 1337, 1455
BstF5I GGATG 8 cut(s) 480, 535, 943, 1012, 1027, 1197, 1199, 1375
BstH2I RGCGCY 1 cut(s) 250
BstHHI GCGC 2 cut(s) 249, 1566
BstKTI GATC 6 cut(s) 97, 346, 643, 981, 1594, 1672
BstMAI GTCTC 4 cut(s) 619, 822, 1456, 1457
BstMBI GATC 6 cut(s) 94, 343, 640, 978, 1591, 1669
BstMWI GCNNNNNNNGC 2 cut(s) 575, 1203
BstNI CCWGG 2 cut(s) 661, 923
BstNSI RCATGY 7 cut(s) 308, 421, 599, 673, 1226, 1412, 1628
BstSCI CCNGG 5 cut(s) 137, 469, 494, 659, 921
BstSFI CTRYAG 1 cut(s) 1149
BstV1I GCAGC 5 cut(s) 32, 35, 450, 556, 801
BstX2I RGATCY 5 cut(s) 343, 640, 978, 1591, 1669
BstXI CCANNNNNNTGG 2 cut(s) 87, 251
BstYI RGATCY 5 cut(s) 343, 640, 978, 1591, 1669
BsuRI GGCC 2 cut(s) 1175, 1552
BtsCI GGATG 8 cut(s) 480, 535, 943, 1012, 1027, 1197, 1199, 1375
BtsIMutI CAGTG 3 cut(s) 752, 775, 929
BveI ACCTGC 1 cut(s) 194
Cac8I GCNNGC 1 cut(s) 306
CaiI CAGNNNCTG 1 cut(s) 1238
CfoI GCGC 2 cut(s) 249, 1566
CseI GACGC 1 cut(s) 820
Csp6I GTAC 4 cut(s) 300, 509, 960, 1384
CspCI CAANNNNNGTGG 4 cut(s) 541, 576, 1569, 1604
CviQI GTAC 4 cut(s) 300, 509, 960, 1384
DdeI CTNAG 5 cut(s) 377, 479, 767, 1337, 1455
DpnI GATC 6 cut(s) 96, 345, 642, 980, 1593, 1671
DpnII GATC 6 cut(s) 94, 343, 640, 978, 1591, 1669
DraI TTTAAA 1 cut(s) 906
Eam1104I CTCTTC 1 cut(s) 1083
EarI CTCTTC 1 cut(s) 1083
EciI GGCGGA 1 cut(s) 76
Ecl136II GAGCTC 2 cut(s) 766, 1522
Eco130I CCWWGG 4 cut(s) 250, 618, 1296, 1547
Eco147I AGGCCT 1 cut(s) 1552
Eco24I GRGCYC 2 cut(s) 768, 1524
Eco31I GGTCTC 1 cut(s) 619
Eco53kI GAGCTC 2 cut(s) 766, 1522
Eco88I CYCGRG 1 cut(s) 1155
EcoICRI GAGCTC 2 cut(s) 766, 1522
EcoRI GAATTC 1 cut(s) 1108
EcoRII CCWGG 2 cut(s) 659, 921
EcoT14I CCWWGG 4 cut(s) 250, 618, 1296, 1547
EcoT22I ATGCAT 4 cut(s) 292, 955, 1199, 1224
EcoT38I GRGCYC 2 cut(s) 768, 1524
ErhI CCWWGG 4 cut(s) 250, 618, 1296, 1547
FalI AAGNNNNNCTT 4 cut(s) 437, 469, 1452, 1484
Fnu4HI GCNGC 6 cut(s) 21, 24, 439, 570, 815, 891
FokI GGATG 8 cut(s) 487, 542, 950, 1019, 1034, 1184, 1206, 1382
FriOI GRGCYC 2 cut(s) 768, 1524
Fsp4HI GCNGC 6 cut(s) 21, 24, 439, 570, 815, 891
FspAI RTGCGCAY 1 cut(s) 1565
FspBI CTAG 5 cut(s) 198, 503, 780, 1419, 1667
FspI TGCGCA 1 cut(s) 1565
GlaI GCGC 2 cut(s) 248, 1565
GluI GCNGC 6 cut(s) 21, 24, 439, 570, 815, 891
GsaI CCCAGC 1 cut(s) 1383
HaeII RGCGCY 1 cut(s) 250
HaeIII GGCC 2 cut(s) 1175, 1552
HapII CCGG 4 cut(s) 139, 370, 470, 495
HgaI GACGC 1 cut(s) 820
HhaI GCGC 2 cut(s) 249, 1566
Hin6I GCGC 2 cut(s) 247, 1564
HinP1I GCGC 2 cut(s) 247, 1564
HincII GTYRAC 1 cut(s) 157
HindII GTYRAC 1 cut(s) 157
HindIII AAGCTT 2 cut(s) 690, 705
HinfI GANTC 4 cut(s) 113, 563, 1066, 1449
HpaII CCGG 4 cut(s) 139, 370, 470, 495
HphI GGTGA 6 cut(s) 24, 104, 218, 538, 548, 1108
Hpy166II GTNNAC 3 cut(s) 157, 799, 1123
Hpy188I TCNGA 2 cut(s) 1056, 1456
Hpy188III TCNNGA 3 cut(s) 317, 644, 1667
Hpy8I GTNNAC 3 cut(s) 157, 799, 1123
Hpy99I CGWCG 1 cut(s) 971
HpyAV CCTTC 3 cut(s) 973, 1184, 1498
HpyCH4III ACNGT 1 cut(s) 1540
HpyCH4IV ACGT 3 cut(s) 123, 354, 1374
HpyF10VI GCNNNNNNNGC 2 cut(s) 575, 1203
HpyF3I CTNAG 5 cut(s) 377, 479, 767, 1337, 1455
HpySE526I ACGT 3 cut(s) 123, 354, 1374
HspAI GCGC 2 cut(s) 247, 1564
KpnI GGTACC 1 cut(s) 963
Kzo9I GATC 6 cut(s) 94, 343, 640, 978, 1591, 1669
LmnI GCTCC 4 cut(s) 295, 763, 1519, 1527
Lsp1109I GCAGC 5 cut(s) 32, 35, 450, 556, 801
LweI GCATC 7 cut(s) 226, 277, 465, 508, 940, 1184, 1206
MaeI CTAG 5 cut(s) 198, 503, 780, 1419, 1667
MaeII ACGT 3 cut(s) 123, 354, 1374
MaeIII GTNAC 6 cut(s) 206, 317, 665, 1182, 1244, 1291
MalI GATC 6 cut(s) 96, 345, 642, 980, 1593, 1671
MboI GATC 6 cut(s) 94, 343, 640, 978, 1591, 1669
MboII GAAGA 4 cut(s) 296, 530, 1100, 1111
MfeI CAATTG 2 cut(s) 993, 1012
MflI RGATCY 5 cut(s) 343, 640, 978, 1591, 1669
MhlI GDGCHC 2 cut(s) 768, 1524
MluCI AATT 9 cut(s) 51, 279, 456, 898, 993, 1012, 1080, 1108, 1573
MlyI GAGTC 2 cut(s) 557, 1458
Mph1103I ATGCAT 4 cut(s) 292, 955, 1199, 1224
MroXI GAANNNNTTC 2 cut(s) 54, 1070
MseI TTAA 5 cut(s) 455, 905, 1572, 1577, 1684
MslI CAYNNNNRTG 1 cut(s) 1309
MspI CCGG 4 cut(s) 139, 370, 470, 495
MspR9I CCNGG 5 cut(s) 139, 471, 496, 661, 923
MunI CAATTG 2 cut(s) 993, 1012
Mva1269I GAATGC 2 cut(s) 455, 1566
MvaI CCWGG 2 cut(s) 661, 923
MwoI GCNNNNNNNGC 2 cut(s) 575, 1203
NciI CCSGG 3 cut(s) 139, 471, 496
NdeII GATC 6 cut(s) 94, 343, 640, 978, 1591, 1669
NlaIV GGNNCC 2 cut(s) 143, 961
NmuCI GTSAC 2 cut(s) 206, 317
NsbI TGCGCA 1 cut(s) 1565
NsiI ATGCAT 4 cut(s) 292, 955, 1199, 1224
NspI RCATGY 7 cut(s) 308, 421, 599, 673, 1226, 1412, 1628
NspV TTCGAA 1 cut(s) 1346
PaeI GCATGC 1 cut(s) 308
PaqCI CACCTGC 1 cut(s) 194
PasI CCCWGGG 1 cut(s) 922
PceI AGGCCT 1 cut(s) 1552
PciI ACATGT 3 cut(s) 417, 669, 1624
PctI GAATGC 2 cut(s) 455, 1566
PdmI GAANNNNTTC 2 cut(s) 54, 1070
PfeI GAWTC 2 cut(s) 113, 1066
Pfl23II CGTACG 1 cut(s) 299
PkrI GCNGC 6 cut(s) 22, 25, 440, 571, 816, 892
PleI GAGTC 2 cut(s) 557, 1457
PpsI GAGTC 2 cut(s) 557, 1457
PscI ACATGT 3 cut(s) 417, 669, 1624
PsiI TTATAA 3 cut(s) 128, 1529, 1602
Psp124BI GAGCTC 2 cut(s) 768, 1524
Psp1406I AACGTT 1 cut(s) 354
Psp6I CCWGG 2 cut(s) 659, 921
PspFI CCCAGC 1 cut(s) 1379
PspGI CCWGG 2 cut(s) 659, 921
PspLI CGTACG 1 cut(s) 299
PspN4I GGNNCC 2 cut(s) 143, 961
PstNI CAGNNNCTG 1 cut(s) 1238
PsuI RGATCY 5 cut(s) 343, 640, 978, 1591, 1669
RsaI GTAC 4 cut(s) 301, 510, 961, 1385
RsaNI GTAC 4 cut(s) 300, 509, 960, 1384
RseI CAYNNNNRTG 1 cut(s) 1309
SacI GAGCTC 2 cut(s) 768, 1524
SaqAI TTAA 5 cut(s) 455, 905, 1572, 1577, 1684
SatI GCNGC 6 cut(s) 21, 24, 439, 570, 815, 891
Sau3AI GATC 6 cut(s) 94, 343, 640, 978, 1591, 1669
SchI GAGTC 2 cut(s) 557, 1458
ScrFI CCNGG 5 cut(s) 139, 471, 496, 661, 923
SduI GDGCHC 2 cut(s) 768, 1524
SfaNI GCATC 7 cut(s) 226, 277, 465, 508, 940, 1184, 1206
SfcI CTRYAG 1 cut(s) 1149
SfuI TTCGAA 1 cut(s) 1346
SmiMI CAYNNNNRTG 1 cut(s) 1309
SmlI CTYRAG 4 cut(s) 224, 612, 644, 974
SmoI CTYRAG 4 cut(s) 224, 612, 644, 974
SpeI ACTAGT 1 cut(s) 197
SphI GCATGC 1 cut(s) 308
Sse9I AATT 9 cut(s) 51, 279, 456, 898, 993, 1012, 1080, 1108, 1573
SseBI AGGCCT 1 cut(s) 1552
SsiI CCGC 2 cut(s) 61, 891
SspI AATATT 1 cut(s) 1047
SspMI CTAG 5 cut(s) 198, 503, 780, 1419, 1667
SstI GAGCTC 2 cut(s) 768, 1524
StuI AGGCCT 1 cut(s) 1552
StyD4I CCNGG 5 cut(s) 137, 469, 494, 659, 921
StyI CCWWGG 4 cut(s) 250, 618, 1296, 1547
TaaI ACNGT 1 cut(s) 1540
TaiI ACGT 3 cut(s) 126, 357, 1377
TaqI TCGA 2 cut(s) 966, 1346
TasI AATT 9 cut(s) 51, 279, 456, 898, 993, 1012, 1080, 1108, 1573
TauI GCSGC 1 cut(s) 893
TfiI GAWTC 2 cut(s) 113, 1066
Tru1I TTAA 5 cut(s) 455, 905, 1572, 1577, 1684
Tru9I TTAA 5 cut(s) 455, 905, 1572, 1577, 1684
TscAI CASTG 3 cut(s) 752, 775, 936
TseFI GTSAC 2 cut(s) 206, 317
TseI GCWGC 5 cut(s) 20, 23, 438, 569, 814
Tsp45I GTSAC 2 cut(s) 206, 317
TspGWI ACGGA 1 cut(s) 354
TspRI CASTG 3 cut(s) 752, 775, 936
XapI RAATTY 2 cut(s) 898, 1108
XbaI TCTAGA 1 cut(s) 1666
XceI RCATGY 7 cut(s) 308, 421, 599, 673, 1226, 1412, 1628
XcmI CCANNNNNNNNNTGG 1 cut(s) 84
XmnI GAANNNNTTC 2 cut(s) 54, 1070
XspI CTAG 5 cut(s) 198, 503, 780, 1419, 1667
Zsp2I ATGCAT 4 cut(s) 292, 955, 1199, 1224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.