RchiOBHm_Chr6g0245751

(-)-germacrene D synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000053
Physical Location & Seq
Forward (+)
1517199 .. 1521030
3832 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22026

Sequence Viewer

Length: 1674 bp
ATGTCAGTCCAAGTTTTGGCATCTCAGGCTCAAAGGCCAGATAGTGTGGCTGATGTTAAGCGACCGTCAGCTAATTTTGCCCCAGGCATTTGGGGTGATCATTTTCTCTCCTATGCTACCACGGAAGTTGACACTAAACTTGAGCAACATGTTCGAGAACTGAAAGAAGAGGTGAAGAGGATGCTAATGGACTCCTTAAAAAACCCTTCACAACAATTGGACTTGGTTGATGACATTCAACGCCTAGGCGTGTCCTATCATTTTGAGAATGAGATTGAAGAAATTTTGAAACAAATTCACCACAACTACTCTTATGGTAGTGAAGATGACCTTTACACTACTGCTCTTTGTTTTCGATTGTTGAGACAACAAGGTTACAATGTTTCATGCGATATGTTCAACAAGTACATGGAAGGAAATGATAAAAAATTTAAGGCATCTCTCCTAAGTGATGTATCCGGACTATTAAGCTTGTATGAAGCCGCGCATCTTAGGATACACGAAGAAGACATACTTGAAGAGGCTCTAGCCTTCACCACCACTCATCTTGAGTCGATCAAACACAGTTTAAACCCTCCACTTTCGAAACAAGTAGCTCATTCCTTAACTCAGCCACTTCGAAAGGGCAACACTAGGGTGGAAGCTAAATATTACTTGTCAGTCTATCATGAACGCGATTCACATAACGAGACTCTCCTTATTTTTGCAAAGTTGGATTTCAACCTACTGCAGCAAGTCCATCAAAAGGAACTATGTGAAATTACACAGTGGTGGAAGGACTTGGACGTGAGAAACACGTTGCCGTTTACAAGAGATAGAGTTACCGAAGTATACTTCGGCTGGGCTTTGTCAGTGTACTTCGAACCTCAATATTCCTTTGCTAGGAGGACATCATGCAAAGTCACCGCCATGGCATCTATTATTGATGACATTTATGATGCTCATGGCACACTCGAAGAATTAGAGCTTTTTACTAAAGCTATTCAGAGGTGGGACGTCTGTGTCATAGATCTGTTACCAGATTATATGAAAGTCTGTTATAAGGCACTATTAGAAGTTTATACTGAAATTGAAGAACAGCTTGCAAAAGAAGGAAAGTTGTATCAAATCCAGTACGCCAGAGAACCTATGAAAAAGCTATTTGAAAATTACTTCCTTGAAGCTAAATGGTTGCACCAAAAGCACATACCAACAATGGATGATTATATGTCCCTAGCATTAATGACCACAGGCTACCCCCTGCTAATAACAACAGCCTTTGTTGGAATGGGAAATATTGCTACACAAGACTCCTTCGATTGGTTGGCCACTTATCCCAAGGCTGTAAAAGGGGCTGCAATAATTTGCCGACTCATGGATGACATAGCGGACCATAAGTTTGAGCAAAAGAGAGGACAAGTTACCTCAGCTGTAGAGTGCTACATGAAAGAATATGGTGCCACAGAAGAAGAAGCAATAATCGGATTGCGTAGAGAAGTGAGTGATGCATGGAAGGACATAAATGAATCGTTCCTCCTCCCTAATGTTCCTCCGAGGCCATTGCTAACTCGAATTATTAACTTTGCATGTATCATGGATGTTGCATACAAGTACGCAGATGGTTACACTACTCATCATGATACTGTGCTTAAGGATTTGATAGTTTCTACACTTGTCCAACCCGTGCCTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

557

Amino Acids

64.36

Weight (kDa)

5.35

Isoelectric Point (pI)

43.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 30 - 202 2.1e-60 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 259 - 499 5.7e-95 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 300 - 499 9.8e-61 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1041
AasI GACNNNNNNGTC 1 cut(s) 1001
AatII GACGTC 1 cut(s) 999
AccB1I GGYRCC 1 cut(s) 1436
AccB7I CCANNNNNTGG 1 cut(s) 16
AccI GTMKAC 1 cut(s) 831
AccII CGCG 2 cut(s) 485, 675
AccIII TCCGGA 1 cut(s) 458
AciI CCGC 3 cut(s) 483, 906, 1367
AcoI YGGCCR 1 cut(s) 1305
AcsI RAATTY 3 cut(s) 282, 294, 428
AcyI GRCGYC 1 cut(s) 996
AfaI GTAC 4 cut(s) 407, 857, 1115, 1592
AfiI CCNNNNNNNGG 5 cut(s) 16, 745, 882, 1299, 1354
AflII CTTAAG 1 cut(s) 1628
AflIII ACRYGT 2 cut(s) 148, 795
AgsI TTSAA 9 cut(s) 239, 278, 289, 400, 518, 721, 1073, 1145, 1160
AjiI CACGTC 1 cut(s) 787
AjnI CCWGG 1 cut(s) 82
AleI CACNNNNGTG 2 cut(s) 635, 769
Alw26I GTCTC 2 cut(s) 358, 683
Aor13HI TCCGGA 1 cut(s) 458
AoxI GGCC 3 cut(s) 35, 1305, 1535
ApeKI GCWGC 2 cut(s) 730, 1334
ApoI RAATTY 3 cut(s) 282, 294, 428
ArsI GACNNNNNNTTYG 2 cut(s) 1361, 1393
AseI ATTAAT 1 cut(s) 1220
AspA2I CCTAGG 1 cut(s) 244
AspLEI GCGC 1 cut(s) 487
AspS9I GGNCC 1 cut(s) 1369
AsuHPI GGTGA 5 cut(s) 107, 184, 290, 526, 895
AsuII TTCGAA 3 cut(s) 584, 619, 861
AvaII GGWCC 1 cut(s) 1369
AvrII CCTAGG 1 cut(s) 244
BalI TGGCCA 1 cut(s) 1307
BanI GGYRCC 1 cut(s) 1436
BarI GAAGNNNNNNTAC 2 cut(s) 495, 527
BbsI GAAGAC 1 cut(s) 513
BbvCI CCTCAGC 1 cut(s) 1405
BbvI GCAGC 2 cut(s) 742, 1321
BccI CCATC 2 cut(s) 747, 1592
BceAI ACGGC 1 cut(s) 787
BcgI CGANNNNNNTGC 4 cut(s) 134, 168, 1522, 1556
BciT130I CCWGG 1 cut(s) 84
BciVI GTATCC 2 cut(s) 466, 489
BclI TGATCA 1 cut(s) 97
BcoDI GTCTC 2 cut(s) 358, 683
BfaI CTAG 5 cut(s) 245, 527, 633, 882, 1214
BfmI CTRYAG 2 cut(s) 728, 1410
BfrI CTTAAG 1 cut(s) 1628
BfuI GTATCC 2 cut(s) 466, 489
BglII AGATCT 1 cut(s) 1009
BisI GCNGC 3 cut(s) 483, 731, 1335
BlnI CCTAGG 1 cut(s) 244
BlsI GCNGC 3 cut(s) 484, 732, 1336
Bme1390I CCNGG 1 cut(s) 84
Bme18I GGWCC 1 cut(s) 1369
BmgBI CACGTC 1 cut(s) 787
BmgT120I GGNCC 1 cut(s) 1369
BmiI GGNNCC 1 cut(s) 1438
BmrFI CCNGG 1 cut(s) 84
BmsI GCATC 7 cut(s) 29, 171, 446, 496, 923, 928, 1474
BpiI GAAGAC 1 cut(s) 513
Bpu10I CCTNAGC 1 cut(s) 1405
Bpu14I TTCGAA 3 cut(s) 584, 619, 861
BpuEI CTTGAG 2 cut(s) 161, 569
BsaHI GRCGYC 1 cut(s) 996
BsaJI CCNNGG 6 cut(s) 82, 120, 244, 909, 1317, 1532
BsaWI WCCGGW 1 cut(s) 458
Bsc4I CCNNNNNNNGG 5 cut(s) 16, 745, 882, 1299, 1354
Bse1I ACTGG 1 cut(s) 1111
Bse3DI GCAATG 1 cut(s) 1538
BseAI TCCGGA 1 cut(s) 458
BseBI CCWGG 1 cut(s) 84
BseDI CCNNGG 6 cut(s) 82, 120, 244, 909, 1317, 1532
BseGI GGATG 4 cut(s) 186, 1204, 1363, 1582
BseLI CCNNNNNNNGG 5 cut(s) 16, 745, 882, 1299, 1354
BseMI GCAATG 1 cut(s) 1538
BseMII CTCAG 3 cut(s) 38, 623, 1419
BseNI ACTGG 1 cut(s) 1111
BseRI GAGGAG 1 cut(s) 1505
BseXI GCAGC 2 cut(s) 742, 1321
BseYI CCCAGC 1 cut(s) 840
Bsh1236I CGCG 2 cut(s) 485, 675
Bsh1285I CGRYCG 1 cut(s) 65
BshFI GGCC 3 cut(s) 37, 1307, 1537
BshNI GGYRCC 1 cut(s) 1436
BsiEI CGRYCG 1 cut(s) 65
BsiSI CCGG 1 cut(s) 459
BslFI GGGAC 2 cut(s) 1007, 1195
BslI CCNNNNNNNGG 5 cut(s) 16, 745, 882, 1299, 1354
BsmAI GTCTC 2 cut(s) 358, 683
BsmFI GGGAC 2 cut(s) 1007, 1195
BsnI GGCC 3 cut(s) 37, 1307, 1537
Bsp119I TTCGAA 3 cut(s) 584, 619, 861
Bsp13I TCCGGA 1 cut(s) 458
Bsp143I GATC 3 cut(s) 97, 555, 1009
Bsp19I CCATGG 1 cut(s) 909
BspACI CCGC 3 cut(s) 483, 906, 1367
BspANI GGCC 3 cut(s) 37, 1307, 1537
BspCNI CTCAG 3 cut(s) 37, 622, 1418
BspEI TCCGGA 1 cut(s) 458
BspFNI CGCG 2 cut(s) 485, 675
BspHI TCATGA 2 cut(s) 667, 1615
BspLI GGNNCC 1 cut(s) 1438
BspMAI CTGCAG 1 cut(s) 732
BspT104I TTCGAA 3 cut(s) 584, 619, 861
BspT107I GGYRCC 1 cut(s) 1436
BspTI CTTAAG 1 cut(s) 1628
BsrDI GCAATG 1 cut(s) 1538
BsrI ACTGG 1 cut(s) 1111
BssECI CCNNGG 6 cut(s) 82, 120, 244, 909, 1317, 1532
BssMI GATC 3 cut(s) 97, 555, 1009
BssNAI GTATAC 1 cut(s) 832
BssNI GRCGYC 1 cut(s) 996
BssT1I CCWWGG 3 cut(s) 244, 909, 1317
Bst1107I GTATAC 1 cut(s) 832
Bst2UI CCWGG 1 cut(s) 84
Bst4CI ACNGT 4 cut(s) 66, 566, 768, 1624
Bst6I CTCTTC 3 cut(s) 162, 170, 513
BstACI GRCGYC 1 cut(s) 996
BstAFI CTTAAG 1 cut(s) 1628
BstBI TTCGAA 3 cut(s) 584, 619, 861
BstC8I GCNNGC 1 cut(s) 1083
BstDEI CTNAG 5 cut(s) 24, 446, 491, 609, 1405
BstDSI CCRYGG 2 cut(s) 120, 909
BstENI CCTNNNNNAGG 1 cut(s) 880
BstF5I GGATG 4 cut(s) 186, 1204, 1363, 1582
BstFNI CGCG 2 cut(s) 485, 675
BstHHI GCGC 1 cut(s) 487
BstKTI GATC 3 cut(s) 100, 558, 1012
BstMAI GTCTC 2 cut(s) 358, 683
BstMBI GATC 3 cut(s) 97, 555, 1009
BstMCI CGRYCG 1 cut(s) 65
BstMWI GCNNNNNNNGC 3 cut(s) 26, 77, 1180
BstNI CCWGG 1 cut(s) 84
BstNSI RCATGY 2 cut(s) 152, 1569
BstSCI CCNGG 1 cut(s) 82
BstSFI CTRYAG 2 cut(s) 728, 1410
BstUI CGCG 2 cut(s) 485, 675
BstV1I GCAGC 2 cut(s) 742, 1321
BstV2I GAAGAC 1 cut(s) 513
BstX2I RGATCY 1 cut(s) 1009
BstXI CCANNNNNNTGG 1 cut(s) 90
BstYI RGATCY 1 cut(s) 1009
BstZ17I GTATAC 1 cut(s) 832
BsuI GTATCC 2 cut(s) 466, 489
BsuRI GGCC 3 cut(s) 37, 1307, 1537
BtgI CCRYGG 2 cut(s) 120, 909
BtrI CACGTC 1 cut(s) 787
BtsCI GGATG 4 cut(s) 186, 1204, 1363, 1582
BtsIMutI CAGTG 2 cut(s) 773, 858
Cac8I GCNNGC 1 cut(s) 1083
CciI TCATGA 2 cut(s) 667, 1615
CfoI GCGC 1 cut(s) 487
Cfr13I GGNCC 1 cut(s) 1369
Csp6I GTAC 4 cut(s) 406, 856, 1114, 1591
CspCI CAANNNNNGTGG 4 cut(s) 109, 144, 529, 564
CviQI GTAC 4 cut(s) 406, 856, 1114, 1591
DdeI CTNAG 5 cut(s) 24, 446, 491, 609, 1405
DpnI GATC 3 cut(s) 99, 557, 1011
DpnII GATC 3 cut(s) 97, 555, 1009
DraI TTTAAA 1 cut(s) 570
DrdI GACNNNNNNGTC 1 cut(s) 1001
DseDI GACNNNNNNGTC 1 cut(s) 1001
EaeI YGGCCR 1 cut(s) 1305
Eam1104I CTCTTC 3 cut(s) 162, 170, 513
EarI CTCTTC 3 cut(s) 162, 170, 513
Eco130I CCWWGG 3 cut(s) 244, 909, 1317
Eco47I GGWCC 1 cut(s) 1369
EcoNI CCTNNNNNAGG 1 cut(s) 880
EcoRII CCWGG 1 cut(s) 82
EcoT14I CCWWGG 3 cut(s) 244, 909, 1317
EcoT22I ATGCAT 1 cut(s) 1489
ErhI CCWWGG 3 cut(s) 244, 909, 1317
FalI AAGNNNNNCTT 6 cut(s) 315, 347, 498, 530, 1065, 1097
FaqI GGGAC 2 cut(s) 1007, 1195
FbaI TGATCA 1 cut(s) 97
FblI GTMKAC 1 cut(s) 831
Fnu4HI GCNGC 3 cut(s) 483, 731, 1335
FokI GGATG 4 cut(s) 193, 1211, 1370, 1589
Fsp4HI GCNGC 3 cut(s) 483, 731, 1335
FspBI CTAG 5 cut(s) 245, 527, 633, 882, 1214
GlaI GCGC 1 cut(s) 486
GluI GCNGC 3 cut(s) 483, 731, 1335
GsaI CCCAGC 1 cut(s) 844
HaeIII GGCC 3 cut(s) 37, 1307, 1537
HapII CCGG 1 cut(s) 459
HhaI GCGC 1 cut(s) 487
Hin1I GRCGYC 1 cut(s) 996
Hin6I GCGC 1 cut(s) 485
HinP1I GCGC 1 cut(s) 485
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HindIII AAGCTT 1 cut(s) 469
HinfI GANTC 7 cut(s) 191, 551, 677, 691, 1289, 1350, 1505
HpaII CCGG 1 cut(s) 459
HphI GGTGA 5 cut(s) 107, 184, 290, 526, 895
Hpy166II GTNNAC 4 cut(s) 130, 807, 832, 856
Hpy188I TCNGA 3 cut(s) 987, 1463, 1533
Hpy188III TCNNGA 5 cut(s) 155, 459, 548, 668, 1616
Hpy8I GTNNAC 4 cut(s) 130, 807, 832, 856
HpyAV CCTTC 7 cut(s) 216, 407, 541, 769, 1085, 1303, 1486
HpyCH4III ACNGT 4 cut(s) 66, 566, 768, 1624
HpyCH4IV ACGT 3 cut(s) 786, 797, 996
HpyCH4V TGCA 9 cut(s) 707, 730, 897, 1085, 1174, 1337, 1487, 1565, 1583
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 77, 1180
HpyF3I CTNAG 5 cut(s) 24, 446, 491, 609, 1405
HpySE526I ACGT 3 cut(s) 786, 797, 996
Hsp92I GRCGYC 1 cut(s) 996
HspAI GCGC 1 cut(s) 485
Kpn2I TCCGGA 1 cut(s) 458
Ksp22I TGATCA 1 cut(s) 97
Kzo9I GATC 3 cut(s) 97, 555, 1009
Lsp1109I GCAGC 2 cut(s) 742, 1321
LweI GCATC 7 cut(s) 29, 171, 446, 496, 923, 928, 1474
MaeI CTAG 5 cut(s) 245, 527, 633, 882, 1214
MaeII ACGT 3 cut(s) 786, 797, 996
MaeIII GTNAC 6 cut(s) 374, 820, 901, 1014, 1399, 1601
MalI GATC 3 cut(s) 99, 557, 1011
MboI GATC 3 cut(s) 97, 555, 1009
MfeI CAATTG 1 cut(s) 215
MflI RGATCY 1 cut(s) 1009
MlsI TGGCCA 1 cut(s) 1307
MluNI TGGCCA 1 cut(s) 1307
MlyI GAGTC 5 cut(s) 185, 560, 685, 1283, 1344
MmeI TCCRAC 2 cut(s) 693, 1243
Mox20I TGGCCA 1 cut(s) 1307
Mph1103I ATGCAT 1 cut(s) 1489
MroI TCCGGA 1 cut(s) 458
MscI TGGCCA 1 cut(s) 1307
MseI TTAA 9 cut(s) 57, 197, 432, 467, 569, 605, 1220, 1557, 1629
MslI CAYNNNNRTG 3 cut(s) 635, 769, 908
Msp20I TGGCCA 1 cut(s) 1307
MspA1I CMGCKG 1 cut(s) 1409
MspCI CTTAAG 1 cut(s) 1628
MspI CCGG 1 cut(s) 459
MspR9I CCNGG 1 cut(s) 84
MssI GTTTAAAC 1 cut(s) 570
MunI CAATTG 1 cut(s) 215
MvaI CCWGG 1 cut(s) 84
MvnI CGCG 2 cut(s) 485, 675
MwoI GCNNNNNNNGC 3 cut(s) 26, 77, 1180
NcoI CCATGG 1 cut(s) 909
NdeII GATC 3 cut(s) 97, 555, 1009
NlaIV GGNNCC 1 cut(s) 1438
NmuCI GTSAC 1 cut(s) 901
NsiI ATGCAT 1 cut(s) 1489
NspI RCATGY 2 cut(s) 152, 1569
NspV TTCGAA 3 cut(s) 584, 619, 861
OliI CACNNNNGTG 2 cut(s) 635, 769
PagI TCATGA 2 cut(s) 667, 1615
PciI ACATGT 1 cut(s) 148
PfeI GAWTC 2 cut(s) 677, 1505
PflMI CCANNNNNTGG 1 cut(s) 16
PkrI GCNGC 3 cut(s) 484, 732, 1336
PleI GAGTC 5 cut(s) 185, 559, 685, 1283, 1344
PmeI GTTTAAAC 1 cut(s) 570
PpsI GAGTC 5 cut(s) 185, 559, 685, 1283, 1344
PscI ACATGT 1 cut(s) 148
PshBI ATTAAT 1 cut(s) 1220
PsiI TTATAA 1 cut(s) 1041
Psp6I CCWGG 1 cut(s) 82
PspFI CCCAGC 1 cut(s) 840
PspGI CCWGG 1 cut(s) 82
PspN4I GGNNCC 1 cut(s) 1438
PspPI GGNCC 1 cut(s) 1369
PstI CTGCAG 1 cut(s) 732
PsuI RGATCY 1 cut(s) 1009
PvuII CAGCTG 1 cut(s) 1409
RsaI GTAC 4 cut(s) 407, 857, 1115, 1592
RsaNI GTAC 4 cut(s) 406, 856, 1114, 1591
RseI CAYNNNNRTG 3 cut(s) 635, 769, 908
SaqAI TTAA 9 cut(s) 57, 197, 432, 467, 569, 605, 1220, 1557, 1629
SatI GCNGC 3 cut(s) 483, 731, 1335
Sau3AI GATC 3 cut(s) 97, 555, 1009
Sau96I GGNCC 1 cut(s) 1369
SchI GAGTC 5 cut(s) 185, 560, 685, 1283, 1344
ScrFI CCNGG 1 cut(s) 84
SfaNI GCATC 7 cut(s) 29, 171, 446, 496, 923, 928, 1474
SfcI CTRYAG 2 cut(s) 728, 1410
SfuI TTCGAA 3 cut(s) 584, 619, 861
SinI GGWCC 1 cut(s) 1369
SmiMI CAYNNNNRTG 3 cut(s) 635, 769, 908
SmlI CTYRAG 3 cut(s) 140, 548, 1628
SmoI CTYRAG 3 cut(s) 140, 548, 1628
SsiI CCGC 3 cut(s) 483, 906, 1367
SspI AATATT 3 cut(s) 650, 872, 1276
SspMI CTAG 5 cut(s) 245, 527, 633, 882, 1214
StyD4I CCNGG 1 cut(s) 82
StyI CCWWGG 3 cut(s) 244, 909, 1317
TaaI ACNGT 4 cut(s) 66, 566, 768, 1624
TaiI ACGT 3 cut(s) 789, 800, 999
TaqI TCGA 9 cut(s) 154, 355, 554, 584, 619, 861, 954, 1296, 1549
TatI WGTACW 2 cut(s) 405, 855
TauI GCSGC 1 cut(s) 485
TfiI GAWTC 2 cut(s) 677, 1505
Tru1I TTAA 9 cut(s) 57, 197, 432, 467, 569, 605, 1220, 1557, 1629
Tru9I TTAA 9 cut(s) 57, 197, 432, 467, 569, 605, 1220, 1557, 1629
TscAI CASTG 2 cut(s) 773, 858
TseFI GTSAC 1 cut(s) 901
TseI GCWGC 2 cut(s) 730, 1334
Tsp45I GTSAC 1 cut(s) 901
TspDTI ATGAA 7 cut(s) 375, 492, 684, 1043, 1145, 1439, 1518
TspGWI ACGGA 1 cut(s) 137
TspRI CASTG 2 cut(s) 773, 858
Van91I CCANNNNNTGG 1 cut(s) 16
Vha464I CTTAAG 1 cut(s) 1628
VpaK11BI GGWCC 1 cut(s) 1369
VspI ATTAAT 1 cut(s) 1220
XagI CCTNNNNNAGG 1 cut(s) 880
XapI RAATTY 3 cut(s) 282, 294, 428
XceI RCATGY 2 cut(s) 152, 1569
XmaJI CCTAGG 1 cut(s) 244
XmiI GTMKAC 1 cut(s) 831
XspI CTAG 5 cut(s) 245, 527, 633, 882, 1214
ZraI GACGTC 1 cut(s) 997
Zsp2I ATGCAT 1 cut(s) 1489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.