MD11G1236600.v1.1

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000053
Physical Location & Seq
Forward (+)
34412423 .. 34414541
2119 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1236600.v1.1.491

Sequence Viewer

Length: 1566 bp
ATGAACAAAACCGAGCGCAATGGCATTACTCAAGCTCACAGTCAACAACAGGTTGATGAACTCAAAAAAGTTGTGAGGGAAGTATTGATAACCAGTGCAACTGATTTTTCACTTCAGCTAAAGTTGATTGATGCAATCCAGCGCCTTGGCGTGGCATACCATTTTGAAAGAGAAATCGAGAAAGCACTTGAACGTATGCATGCTACATTTGATGGTGACCATGGCAACAATGATGTTGATCTATACACTGTTGCTCTTGGTTTCCGGCTGCTAAGACAACACGGGTATAATGTTTCATGTGCCATATTCACCAATTTCAAAGATAAAAGTAGTAGCTTCAAGGAAAGCTTGATTGATGACGTGTCTGGTATGCTAAGCTTTTATGAAGCAACACATCTGAGGGCGCATGGAGAAGACATATTAGAAGAGGCTCTTGTATTCACCACCACTCACCTTGAGTCAGCAGCAACCCGTGTAAGCAATCCACTCGCTACACAAATAACTCAAGCCCTAGAGAGACCCCTGCGAAAAAGTCTAGAGAGATTATCTGCGCGACGTTATACTTCAATCTACCAAGAAGAAGCTTCACATAATGTCTCTCTTCTGAAACTTGCAAAGTTAGACTTCAATCTTGTTCAGCTTTTGCACAAAAAGGAGCTGCAAGAGATTACTAGAGCACTAGACTTTGAAAGGAAGCTGCCATTTGCAAGAGATAGGATGGTAGAGTTGTACTTTTGGATCGTCGGAGTGTATTTTGAACCCCAGTACTCTGTTGGGAGAAAAATTATGACAAAAGCAAGTGTCTTGCTGACAACATTGGATGATATCTACGATGCATTTGGTACATTTGAAGAGCTCGTAGTCTTTACTGAAGCGATTGACAGGTGGGATGTCAATTACATTGATGAACTCCCGGAATATATGCAAACATTTTATCATGCACTTTTAAATCTCTACAATGATATTGAGGCAGAGATGGCGAAGGAAGGAAGATCATACCGAGTTCCATATGCAATACAAGCTATGAAAGATCAAGCTCGATCTTATTTTAATGAGGCCCGATGGTTGCACGAGGGACGAGTTCCAAGTATGGAGGAGTATATGCGCGTTGCAACAGTTTCGATTAGTTACACCTTTCTTACAACCATATCTTTACTTGGCATGGGAGATGTTGTGACAAAGGAAGCATTTGAGTGGTTGTTCACTGACCCCAAAATTGTTAGAGCTGCAAATACCATTTTTAGGCTCATGAATGACATTGTTTCGACAAACTTTGAGAAAGAGAGAGGGCATGCTGCTTCTAGTGTTGATTGCTACATGAAGCAATATGGGGTGTCAGAGCAAGAGACAGTTGATGTTTTTAAGAAACAAATTATGGATTTGTGGAAGGATATAAATGCGGAGTTCTTTAGACCAACTTCTGTGTCAATGCATGTTCTTATGCGCGTTCTCAATTTAACAAGAGTAGTTGATCTTCTTTACAAAAAGGAAGATGGATTCACACATGTGGGGAAGGTGATGAAAGATAGTGTTGCTTCAATTTGTATCGATCCATTGCCACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

522

Amino Acids

60.19

Weight (kDa)

5.62

Isoelectric Point (pI)

33.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 9 - 171 1.6e-55 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 227 - 464 2e-98 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 267 - 464 2.4e-61 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 553, 1107, 1446
AciI CCGC 1 cut(s) 1400
AclWI GGATC 2 cut(s) 746, 1544
AcuI CTGAAG 2 cut(s) 98, 891
AfaI GTAC 3 cut(s) 731, 767, 844
AfiI CCNNNNNNNGG 2 cut(s) 151, 1242
AflIII ACRYGT 2 cut(s) 360, 1504
AjiI CACGTC 1 cut(s) 361
AleI CACNNNNGTG 1 cut(s) 1505
Alw21I GWGCWC 2 cut(s) 679, 858
Alw26I GTCTC 3 cut(s) 511, 601, 1340
AlwI GGATC 2 cut(s) 746, 1544
AoxI GGCC 1 cut(s) 1056
ApeKI GCWGC 6 cut(s) 268, 464, 658, 697, 1226, 1295
AspLEI GCGC 6 cut(s) 18, 144, 406, 553, 1107, 1446
AspS9I GGNCC 1 cut(s) 1057
AsuC2I CCSGG 1 cut(s) 914
AsuHPI GGTGA 5 cut(s) 227, 301, 433, 443, 1528
BanII GRGCYC 1 cut(s) 858
BarI GAAGNNNNNNTAC 2 cut(s) 1458, 1490
BauI CACGAG 1 cut(s) 1070
BbsI GAAGAC 1 cut(s) 420
Bbv12I GWGCWC 2 cut(s) 679, 858
BbvI GCAGC 6 cut(s) 255, 476, 645, 684, 1213, 1282
BccI CCATC 5 cut(s) 206, 712, 970, 1056, 1487
BcgI CGANNNNNNTGC 4 cut(s) 469, 503, 1101, 1135
BcnI CCSGG 1 cut(s) 914
BcoDI GTCTC 3 cut(s) 511, 601, 1340
BfaI CTAG 5 cut(s) 512, 536, 672, 680, 1302
BfoI RGCGCY 1 cut(s) 145
BisI GCNGC 6 cut(s) 269, 465, 659, 698, 1227, 1296
BlpI GCTNAGC 1 cut(s) 374
BlsI GCNGC 6 cut(s) 270, 466, 660, 699, 1228, 1297
BmcAI AGTACT 1 cut(s) 767
Bme1390I CCNGG 1 cut(s) 914
BmgBI CACGTC 1 cut(s) 361
BmgT120I GGNCC 1 cut(s) 1057
BmrFI CCNGG 1 cut(s) 914
BmrI ACTGGG 1 cut(s) 757
BmsI GCATC 2 cut(s) 121, 823
BmuI ACTGGG 1 cut(s) 757
BpiI GAAGAC 1 cut(s) 420
Bpu1102I GCTNAGC 1 cut(s) 374
BpuEI CTTGAG 3 cut(s) 15, 476, 489
BpuMI CCSGG 1 cut(s) 914
Bsa29I ATCGAT 1 cut(s) 1548
BsaBI GATNNNNATC 1 cut(s) 237
BsaI GGTCTC 1 cut(s) 511
BsaJI CCNNGG 2 cut(s) 145, 220
BsaXI ACNNNNNCTCC 2 cut(s) 1158, 1188
Bsc4I CCNNNNNNNGG 2 cut(s) 151, 1242
Bse1I ACTGG 2 cut(s) 93, 763
Bse3DI GCAATG 2 cut(s) 25, 1553
Bse8I GATNNNNATC 1 cut(s) 237
BseCI ATCGAT 1 cut(s) 1548
BseDI CCNNGG 2 cut(s) 145, 220
BseGI GGATG 3 cut(s) 723, 826, 895
BseJI GATNNNNATC 1 cut(s) 237
BseLI CCNNNNNNNGG 2 cut(s) 151, 1242
BseMI GCAATG 2 cut(s) 25, 1553
BseMII CTCAG 1 cut(s) 389
BseNI ACTGG 2 cut(s) 93, 763
BseRI GAGGAG 1 cut(s) 1109
BseXI GCAGC 6 cut(s) 255, 476, 645, 684, 1213, 1282
Bsh1236I CGCG 3 cut(s) 553, 1107, 1446
BshFI GGCC 1 cut(s) 1058
BshVI ATCGAT 1 cut(s) 1548
BsiHKAI GWGCWC 2 cut(s) 679, 858
BsiSI CCGG 2 cut(s) 265, 914
BslFI GGGAC 1 cut(s) 1089
BslI CCNNNNNNNGG 2 cut(s) 151, 1242
BsmAI GTCTC 3 cut(s) 511, 601, 1340
BsmFI GGGAC 1 cut(s) 1089
BsnI GGCC 1 cut(s) 1058
Bso31I GGTCTC 1 cut(s) 511
Bsp1286I GDGCHC 2 cut(s) 679, 858
Bsp143I GATC 7 cut(s) 238, 738, 992, 1030, 1040, 1471, 1549
Bsp1720I GCTNAGC 1 cut(s) 374
Bsp19I CCATGG 1 cut(s) 220
BspACI CCGC 1 cut(s) 1400
BspANI GGCC 1 cut(s) 1058
BspCNI CTCAG 1 cut(s) 390
BspDI ATCGAT 1 cut(s) 1548
BspFNI CGCG 3 cut(s) 553, 1107, 1446
BspHI TCATGA 1 cut(s) 1248
BspPI GGATC 2 cut(s) 746, 1544
BspQI GCTCTTC 1 cut(s) 846
BspTNI GGTCTC 1 cut(s) 511
BsrDI GCAATG 2 cut(s) 25, 1553
BsrI ACTGG 2 cut(s) 93, 763
BssECI CCNNGG 2 cut(s) 145, 220
BssMI GATC 7 cut(s) 238, 738, 992, 1030, 1040, 1471, 1549
BssSI CACGAG 1 cut(s) 1070
BssT1I CCWWGG 2 cut(s) 145, 220
Bst2BI CACGAG 1 cut(s) 1070
Bst4CI ACNGT 4 cut(s) 41, 250, 1117, 1351
Bst6I CTCTTC 3 cut(s) 420, 606, 846
BstC8I GCNNGC 2 cut(s) 201, 1293
BstDEI CTNAG 3 cut(s) 272, 374, 398
BstDSI CCRYGG 1 cut(s) 220
BstEII GGTNACC 1 cut(s) 215
BstF5I GGATG 3 cut(s) 723, 826, 895
BstFNI CGCG 3 cut(s) 553, 1107, 1446
BstH2I RGCGCY 1 cut(s) 145
BstHHI GCGC 6 cut(s) 18, 144, 406, 553, 1107, 1446
BstKTI GATC 7 cut(s) 241, 741, 995, 1033, 1043, 1474, 1552
BstMAI GTCTC 3 cut(s) 511, 601, 1340
BstMBI GATC 7 cut(s) 238, 738, 992, 1030, 1040, 1471, 1549
BstMWI GCNNNNNNNGC 2 cut(s) 977, 1019
BstNSI RCATGY 4 cut(s) 203, 1295, 1436, 1508
BstPI GGTNACC 1 cut(s) 215
BstSCI CCNGG 1 cut(s) 912
BstUI CGCG 3 cut(s) 553, 1107, 1446
BstV1I GCAGC 6 cut(s) 255, 476, 645, 684, 1213, 1282
BstV2I GAAGAC 1 cut(s) 420
BstXI CCANNNNNNTGG 1 cut(s) 146
Bsu15I ATCGAT 1 cut(s) 1548
BsuRI GGCC 1 cut(s) 1058
BsuTUI ATCGAT 1 cut(s) 1548
BtgI CCRYGG 1 cut(s) 220
BtrI CACGTC 1 cut(s) 361
BtsCI GGATG 3 cut(s) 723, 826, 895
BtsIMutI CAGTG 3 cut(s) 100, 246, 1203
Cac8I GCNNGC 2 cut(s) 201, 1293
CciI TCATGA 1 cut(s) 1248
CfoI GCGC 6 cut(s) 18, 144, 406, 553, 1107, 1446
Cfr13I GGNCC 1 cut(s) 1057
ClaI ATCGAT 1 cut(s) 1548
Csp6I GTAC 3 cut(s) 730, 766, 843
CspCI CAANNNNNGTGG 2 cut(s) 436, 471
CviQI GTAC 3 cut(s) 730, 766, 843
DdeI CTNAG 3 cut(s) 272, 374, 398
DpnI GATC 7 cut(s) 240, 740, 994, 1032, 1042, 1473, 1551
DpnII GATC 7 cut(s) 238, 738, 992, 1030, 1040, 1471, 1549
DraI TTTAAA 1 cut(s) 948
Eam1104I CTCTTC 3 cut(s) 420, 606, 846
EarI CTCTTC 3 cut(s) 420, 606, 846
Ecl136II GAGCTC 1 cut(s) 856
Eco130I CCWWGG 2 cut(s) 145, 220
Eco24I GRGCYC 1 cut(s) 858
Eco31I GGTCTC 1 cut(s) 511
Eco32I GATATC 1 cut(s) 826
Eco53kI GAGCTC 1 cut(s) 856
Eco57I CTGAAG 2 cut(s) 98, 891
Eco91I GGTNACC 1 cut(s) 215
EcoICRI GAGCTC 1 cut(s) 856
EcoO65I GGTNACC 1 cut(s) 215
EcoRV GATATC 1 cut(s) 826
EcoT14I CCWWGG 2 cut(s) 145, 220
EcoT22I ATGCAT 3 cut(s) 201, 838, 1434
EcoT38I GRGCYC 1 cut(s) 858
ErhI CCWWGG 2 cut(s) 145, 220
FalI AAGNNNNNCTT 6 cut(s) 332, 364, 417, 449, 608, 640
FaqI GGGAC 1 cut(s) 1089
FauNDI CATATG 1 cut(s) 1009
Fnu4HI GCNGC 6 cut(s) 269, 465, 659, 698, 1227, 1296
FokI GGATG 3 cut(s) 730, 833, 902
FriOI GRGCYC 1 cut(s) 858
Fsp4HI GCNGC 6 cut(s) 269, 465, 659, 698, 1227, 1296
FspBI CTAG 5 cut(s) 512, 536, 672, 680, 1302
GlaI GCGC 6 cut(s) 17, 143, 405, 552, 1106, 1445
GluI GCNGC 6 cut(s) 269, 465, 659, 698, 1227, 1296
HaeII RGCGCY 1 cut(s) 145
HaeIII GGCC 1 cut(s) 1058
HapII CCGG 2 cut(s) 265, 914
HhaI GCGC 6 cut(s) 18, 144, 406, 553, 1107, 1446
Hin6I GCGC 6 cut(s) 16, 142, 404, 551, 1105, 1444
HinP1I GCGC 6 cut(s) 16, 142, 404, 551, 1105, 1444
HincII GTYRAC 1 cut(s) 44
HindII GTYRAC 1 cut(s) 44
HindIII AAGCTT 3 cut(s) 346, 376, 582
HinfI GANTC 2 cut(s) 458, 1497
HpaII CCGG 2 cut(s) 265, 914
HphI GGTGA 5 cut(s) 227, 301, 433, 443, 1528
Hpy166II GTNNAC 2 cut(s) 44, 1203
Hpy188I TCNGA 4 cut(s) 399, 606, 746, 1339
Hpy188III TCNNGA 3 cut(s) 178, 536, 1249
Hpy8I GTNNAC 2 cut(s) 44, 1203
Hpy99I CGWCG 2 cut(s) 558, 746
HpyAV CCTTC 4 cut(s) 976, 980, 1381, 1507
HpyCH4III ACNGT 4 cut(s) 41, 250, 1117, 1351
HpyCH4IV ACGT 3 cut(s) 193, 360, 556
HpyF10VI GCNNNNNNNGC 2 cut(s) 977, 1019
HpyF3I CTNAG 3 cut(s) 272, 374, 398
HpySE526I ACGT 3 cut(s) 193, 360, 556
HspAI GCGC 6 cut(s) 16, 142, 404, 551, 1105, 1444
Kzo9I GATC 7 cut(s) 238, 738, 992, 1030, 1040, 1471, 1549
LguI GCTCTTC 1 cut(s) 846
LmnI GCTCC 1 cut(s) 655
LpnPI CCDG 9 cut(s) 35, 106, 152, 278, 351, 536, 776, 868, 927
Lsp1109I GCAGC 6 cut(s) 255, 476, 645, 684, 1213, 1282
LweI GCATC 2 cut(s) 121, 823
MaeI CTAG 5 cut(s) 512, 536, 672, 680, 1302
MaeII ACGT 3 cut(s) 193, 360, 556
MaeIII GTNAC 3 cut(s) 215, 1127, 1174
MalI GATC 7 cut(s) 240, 740, 994, 1032, 1042, 1473, 1551
MboI GATC 7 cut(s) 238, 738, 992, 1030, 1040, 1471, 1549
MboII GAAGA 8 cut(s) 425, 437, 590, 593, 863, 1002, 1466, 1502
MhlI GDGCHC 2 cut(s) 679, 858
MluCI AATT 7 cut(s) 313, 783, 895, 1215, 1371, 1453, 1539
MlyI GAGTC 1 cut(s) 467
MmeI TCCRAC 1 cut(s) 724
MnlI CCTC 8 cut(s) 69, 393, 421, 961, 1048, 1066, 1087, 1280
Mph1103I ATGCAT 3 cut(s) 201, 838, 1434
MseI TTAA 4 cut(s) 947, 1050, 1362, 1457
MslI CAYNNNNRTG 2 cut(s) 1192, 1505
MspI CCGG 2 cut(s) 265, 914
MspR9I CCNGG 1 cut(s) 914
MvnI CGCG 3 cut(s) 553, 1107, 1446
MwoI GCNNNNNNNGC 2 cut(s) 977, 1019
NciI CCSGG 1 cut(s) 914
NcoI CCATGG 1 cut(s) 220
NdeI CATATG 1 cut(s) 1009
NdeII GATC 7 cut(s) 238, 738, 992, 1030, 1040, 1471, 1549
NmuCI GTSAC 2 cut(s) 215, 1174
NsiI ATGCAT 3 cut(s) 201, 838, 1434
NspI RCATGY 4 cut(s) 203, 1295, 1436, 1508
OliI CACNNNNGTG 1 cut(s) 1505
PaeI GCATGC 2 cut(s) 203, 1295
PagI TCATGA 1 cut(s) 1248
PciI ACATGT 1 cut(s) 1504
PciSI GCTCTTC 1 cut(s) 846
PfeI GAWTC 1 cut(s) 1497
PfoI TCCNGGA 1 cut(s) 912
PkrI GCNGC 6 cut(s) 270, 466, 660, 699, 1228, 1297
PleI GAGTC 1 cut(s) 466
PpsI GAGTC 1 cut(s) 466
PscI ACATGT 1 cut(s) 1504
Psp124BI GAGCTC 1 cut(s) 858
PspEI GGTNACC 1 cut(s) 215
PspPI GGNCC 1 cut(s) 1057
RsaI GTAC 3 cut(s) 731, 767, 844
RsaNI GTAC 3 cut(s) 730, 766, 843
RseI CAYNNNNRTG 2 cut(s) 1192, 1505
SacI GAGCTC 1 cut(s) 858
SapI GCTCTTC 1 cut(s) 846
SaqAI TTAA 4 cut(s) 947, 1050, 1362, 1457
SatI GCNGC 6 cut(s) 269, 465, 659, 698, 1227, 1296
Sau3AI GATC 7 cut(s) 238, 738, 992, 1030, 1040, 1471, 1549
Sau96I GGNCC 1 cut(s) 1057
ScaI AGTACT 1 cut(s) 767
SchI GAGTC 1 cut(s) 467
ScrFI CCNGG 1 cut(s) 914
SduI GDGCHC 2 cut(s) 679, 858
SfaNI GCATC 2 cut(s) 121, 823
SmiMI CAYNNNNRTG 2 cut(s) 1192, 1505
SmlI CTYRAG 3 cut(s) 30, 455, 504
SmoI CTYRAG 3 cut(s) 30, 455, 504
SphI GCATGC 2 cut(s) 203, 1295
Sse9I AATT 7 cut(s) 313, 783, 895, 1215, 1371, 1453, 1539
SsiI CCGC 1 cut(s) 1400
SspMI CTAG 5 cut(s) 512, 536, 672, 680, 1302
SstI GAGCTC 1 cut(s) 858
StyD4I CCNGG 1 cut(s) 912
StyI CCWWGG 2 cut(s) 145, 220
TaaI ACNGT 4 cut(s) 41, 250, 1117, 1351
TaiI ACGT 3 cut(s) 196, 363, 559
TaqI TCGA 5 cut(s) 177, 1039, 1121, 1265, 1548
TasI AATT 7 cut(s) 313, 783, 895, 1215, 1371, 1453, 1539
TatI WGTACW 2 cut(s) 729, 765
TfiI GAWTC 1 cut(s) 1497
Tru1I TTAA 4 cut(s) 947, 1050, 1362, 1457
Tru9I TTAA 4 cut(s) 947, 1050, 1362, 1457
TscAI CASTG 3 cut(s) 100, 253, 1210
TseFI GTSAC 2 cut(s) 215, 1174
TseI GCWGC 6 cut(s) 268, 464, 658, 697, 1226, 1295
Tsp45I GTSAC 2 cut(s) 215, 1174
TspDTI ATGAA 9 cut(s) 17, 72, 285, 399, 921, 1040, 1265, 1334, 1535
TspRI CASTG 3 cut(s) 100, 253, 1210
XbaI TCTAGA 1 cut(s) 535
XceI RCATGY 4 cut(s) 203, 1295, 1436, 1508
XcmI CCANNNNNNNNNTGG 1 cut(s) 770
XspI CTAG 5 cut(s) 512, 536, 672, 680, 1302
ZrmI AGTACT 1 cut(s) 767
Zsp2I ATGCAT 3 cut(s) 201, 838, 1434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.