Prupe.4G198200_v2.0.a1

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000053
Physical Location & Seq
Forward (+)
12162746 .. 12165509
2764 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G198200.2

Sequence Viewer

Length: 1749 bp
ATGAACAATACTTGCAGAGCAAAAAAGATCTCTGACATTTTTACACAAGTACAAATCATGGCTCTAGAAGCTGGTTCAACAGCTCAATCACAAAATTCTAAGGCTGATCAAATTGTTCGACGGACAGCAAATTACCGCCCGAGCATTTGGGGGGATCAGTTTATGAATTATGTTTCAGAAGACATTATAACTTATGCCCATAAGCAACAAGAAGTTGAAGAACTGAAACTAGCAGTGAGGAGGGAAGTGTTCACAACCAGTGCAGATGATTTTTCAAATCAGATGAAGCTCATTGATGCAATCCAGCGCCTTGGCGTGGCGTACCACTTTGAAAAGGAAATAGAAGAAGCACTGGAACATATATATGCTGCAAATAATTTCCATGATGATGATGGTGATGGTGACCTATACAATGTTTCCCTTGGTTTTCGGCTACTAAGACAACATGGACATAATGCTTCATGCAGAATATTCAACAAGTTCAAAGAAAGTAAAAATGGAGGCTTCAAGGAAAGCTTAATCGCTGATGTGCCGGGAATGCTAAGCTTTTATGAAGCAACGCATTTGAGGGTGCATGGCGAAGATATACTAGAAGAGGCTCTTGTTTTCACGACAAAACATCTGGAGTCGGCAACAACCCATGTAAGCTATCAATTGGCAGAACAAATAGCTCAAGCCCTAGAGAGACCGCTCCGAAAGAGTCTCGAGAGGTTATGCGCCCGTCGGTTCATGTCCATCTACCAAGATGAAGCTTCACATAATGAAGCTCTATTAAAACTTGCCAAGTCAGATTTCAATCTTGTTCAGTCTTTACACAAACAAGAGCTCAGTGAAATTATTAGGTGGTGGAAAGAACTAGACTTTGGAAGGAAGCTGCCTTTTGCAAGAAACAGAATTGCGGAGTTATACTGCTGGATTTTGGGGGTCTATTTTGAGCCCCAATACCTTGTTGGAAGAAAATTTCTCACAAAAATTATTGCCCTGATGTCTGTAATGGATGATATCTATGATGCATTCGGTACATTTGAAGAACTTGAGATCTTTACGGAAGCAATTCATCAAAGATGGCATGCCAATTGCATGGATGGACTGCCAGACTACATGCAAACATTCTTCCATGCGCTTTTGAATGTTTTCAATGAAATTGAGGAGGAGATGGTGAAGGAAGGAAGAGCATACCGAGCTCACTATGCAAAAGAAGCGTGGAAAACTATTACCAAAGCTTACTTTGATGAGGCCAAATGGTTCCACGAAGGATGCATCCCAAGCATGGAGGAGTATATGCGTGTTGCTGCAACTTCTGCTGCTAGCTTCGCACTTTCAGCTACATCTTTAGTTGGCATGGGAGATATTGTAACCAAGGAGTCCTTTGAGTGGTTGTTCAATGACCCTAAAATTCTTAGAGCTTCCAATATCATTGTTAGGCTCATGGATGACATCGTGTCGAGCAAGTTTGAAAAAGAGAGAGGCCATGTTGCTTGTGCTATTGATTGTTACATGAAGCAATATGGGGTCTCAAATGAGCAAAAGATAATTGATGTCTTCAACAAACAAATTGTAGATTCGTGGAAAGATATCAACGAGGAGTTCCTTAGACCAACTTCTATGCCGATGCCTATCCTTGAACGTATTGTTAATTTAACAAGAGTGGTGGATCTCCTCTACAAAAAAAATGATGGCTACACACATGCTGGAAAAGTGATGAACGATTGCGTCGCTTCGTATTTTATTGATCCGGCACCAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

583

Amino Acids

67.26

Weight (kDa)

5.58

Isoelectric Point (pI)

45.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 188
AccB1I GGYRCC 1 cut(s) 1738
AccBSI CCGCTC 1 cut(s) 691
AciI CCGC 3 cut(s) 136, 689, 899
AclWI GGATC 3 cut(s) 162, 1662, 1727
AcsI RAATTY 3 cut(s) 94, 959, 1395
AfaI GTAC 3 cut(s) 51, 323, 1021
AfiI CCNNNNNNNGG 3 cut(s) 316, 1270, 1374
AhdI GACNNNNNGTC 1 cut(s) 1441
AjuI GAANNNNNNNTTGG 4 cut(s) 776, 808, 846, 878
Alw21I GWGCWC 2 cut(s) 828, 1186
Alw26I GTCTC 3 cut(s) 679, 707, 1519
AlwI GGATC 3 cut(s) 162, 1662, 1727
Ama87I CYCGRG 2 cut(s) 139, 704
AoxI GGCC 2 cut(s) 1236, 1468
ApeKI GCWGC 4 cut(s) 368, 874, 1292, 1304
ApoI RAATTY 3 cut(s) 94, 959, 1395
Asp700I GAANNNNTTC 2 cut(s) 1053, 1133
AspLEI GCGC 3 cut(s) 309, 719, 1123
AsuC2I CCSGG 1 cut(s) 534
AsuHPI GGTGA 3 cut(s) 407, 413, 1171
AsuNHI GCTAGC 1 cut(s) 1307
AvaI CYCGRG 2 cut(s) 139, 704
BanI GGYRCC 1 cut(s) 1738
BanII GRGCYC 3 cut(s) 828, 939, 1186
BbsI GAAGAC 2 cut(s) 186, 1534
Bbv12I GWGCWC 2 cut(s) 828, 1186
BbvI GCAGC 4 cut(s) 355, 861, 1279, 1291
BccI CCATC 7 cut(s) 386, 392, 743, 1059, 1079, 1150, 1670
BclI TGATCA 1 cut(s) 106
BcnI CCSGG 1 cut(s) 534
BcoDI GTCTC 3 cut(s) 679, 707, 1519
BfaI CTAG 6 cut(s) 65, 230, 590, 680, 857, 1308
BfoI RGCGCY 1 cut(s) 310
BglII AGATCT 2 cut(s) 27, 1038
BisI GCNGC 4 cut(s) 369, 875, 1293, 1305
BlpI GCTNAGC 1 cut(s) 542
BlsI GCNGC 4 cut(s) 370, 876, 1294, 1306
Bme1390I CCNGG 1 cut(s) 534
BmeRI GACNNNNNGTC 1 cut(s) 1441
BmeT110I CYCGRG 2 cut(s) 139, 704
BmiI GGNNCC 2 cut(s) 1247, 1740
BmrFI CCNGG 1 cut(s) 534
BmsI GCATC 5 cut(s) 286, 1000, 1247, 1269, 1602
BmtI GCTAGC 1 cut(s) 1311
BpiI GAAGAC 2 cut(s) 186, 1534
BpmI CTGGAG 1 cut(s) 644
Bpu1102I GCTNAGC 1 cut(s) 542
BpuEI CTTGAG 2 cut(s) 657, 1055
BpuMI CCSGG 1 cut(s) 534
BsaBI GATNNNNATC 2 cut(s) 795, 1616
BsaI GGTCTC 2 cut(s) 679, 1519
BsaJI CCNNGG 3 cut(s) 310, 421, 1359
BsaXI ACNNNNNCTCC 2 cut(s) 1338, 1368
Bsc4I CCNNNNNNNGG 3 cut(s) 316, 1270, 1374
Bse1I ACTGG 3 cut(s) 258, 357, 1742
Bse8I GATNNNNATC 2 cut(s) 795, 1616
BseDI CCNNGG 3 cut(s) 310, 421, 1359
BseGI GGATG 5 cut(s) 1003, 1090, 1260, 1262, 1438
BseJI GATNNNNATC 2 cut(s) 795, 1616
BseLI CCNNNNNNNGG 3 cut(s) 316, 1270, 1374
BseMII CTCAG 1 cut(s) 841
BseNI ACTGG 3 cut(s) 258, 357, 1742
BseRI GAGGAG 6 cut(s) 253, 1163, 1166, 1289, 1598, 1649
BseXI GCAGC 4 cut(s) 355, 861, 1279, 1291
BsgI GTGCAG 1 cut(s) 282
BshFI GGCC 2 cut(s) 1238, 1470
BshNI GGYRCC 1 cut(s) 1738
BsiHKAI GWGCWC 2 cut(s) 828, 1186
BsiHKCI CYCGRG 2 cut(s) 139, 704
BsiSI CCGG 2 cut(s) 533, 1736
BslI CCNNNNNNNGG 3 cut(s) 316, 1270, 1374
BsmAI GTCTC 3 cut(s) 679, 707, 1519
BsmI GAATGC 2 cut(s) 543, 1013
BsnI GGCC 2 cut(s) 1238, 1470
Bso31I GGTCTC 2 cut(s) 679, 1519
BsoBI CYCGRG 2 cut(s) 139, 704
Bsp1286I GDGCHC 3 cut(s) 828, 939, 1186
Bsp143I GATC 6 cut(s) 27, 106, 154, 1038, 1654, 1732
Bsp1720I GCTNAGC 1 cut(s) 542
BspACI CCGC 3 cut(s) 136, 689, 899
BspANI GGCC 2 cut(s) 1238, 1470
BspCNI CTCAG 1 cut(s) 840
BspLI GGNNCC 2 cut(s) 1247, 1740
BspOI GCTAGC 1 cut(s) 1311
BspPI GGATC 3 cut(s) 162, 1662, 1727
BspQI GCTCTTC 1 cut(s) 1165
BspT107I GGYRCC 1 cut(s) 1738
BspTNI GGTCTC 2 cut(s) 679, 1519
BsrBI CCGCTC 1 cut(s) 691
BsrI ACTGG 3 cut(s) 258, 357, 1742
BssECI CCNNGG 3 cut(s) 310, 421, 1359
BssMI GATC 6 cut(s) 27, 106, 154, 1038, 1654, 1732
BssT1I CCWWGG 3 cut(s) 310, 421, 1359
Bst6I CTCTTC 2 cut(s) 588, 1165
BstAPI GCANNNNNTGC 1 cut(s) 1301
BstC8I GCNNGC 2 cut(s) 1071, 1309
BstDEI CTNAG 6 cut(s) 99, 437, 542, 827, 1400, 1592
BstEII GGTNACC 1 cut(s) 401
BstF5I GGATG 5 cut(s) 1003, 1090, 1260, 1262, 1438
BstH2I RGCGCY 1 cut(s) 310
BstHHI GCGC 3 cut(s) 309, 719, 1123
BstKTI GATC 6 cut(s) 30, 109, 157, 1041, 1657, 1735
BstMAI GTCTC 3 cut(s) 679, 707, 1519
BstMBI GATC 6 cut(s) 27, 106, 154, 1038, 1654, 1732
BstMWI GCNNNNNNNGC 9 cut(s) 68, 538, 1181, 1190, 1199, 1266, 1301, 1313, 1322
BstNSI RCATGY 3 cut(s) 1073, 1105, 1691
BstPI GGTNACC 1 cut(s) 401
BstSCI CCNGG 1 cut(s) 532
BstV1I GCAGC 4 cut(s) 355, 861, 1279, 1291
BstV2I GAAGAC 2 cut(s) 186, 1534
BstX2I RGATCY 3 cut(s) 27, 1038, 1654
BstXI CCANNNNNNTGG 2 cut(s) 311, 1081
BstYI RGATCY 3 cut(s) 27, 1038, 1654
BsuRI GGCC 2 cut(s) 1238, 1470
BtsCI GGATG 5 cut(s) 1003, 1090, 1260, 1262, 1438
BtsI GCAGTG 1 cut(s) 240
BtsIMutI CAGTG 4 cut(s) 240, 265, 350, 835
Cac8I GCNNGC 2 cut(s) 1071, 1309
CfoI GCGC 3 cut(s) 309, 719, 1123
CseI GACGC 1 cut(s) 1702
Csp6I GTAC 3 cut(s) 50, 322, 1020
CspCI CAANNNNNGTGG 2 cut(s) 1632, 1667
CviQI GTAC 3 cut(s) 50, 322, 1020
DdeI CTNAG 6 cut(s) 99, 437, 542, 827, 1400, 1592
DpnI GATC 6 cut(s) 29, 108, 156, 1040, 1656, 1734
DpnII GATC 6 cut(s) 27, 106, 154, 1038, 1654, 1732
DriI GACNNNNNGTC 1 cut(s) 1441
Eam1104I CTCTTC 2 cut(s) 588, 1165
Eam1105I GACNNNNNGTC 1 cut(s) 1441
EarI CTCTTC 2 cut(s) 588, 1165
Ecl136II GAGCTC 2 cut(s) 826, 1184
Eco130I CCWWGG 3 cut(s) 310, 421, 1359
Eco24I GRGCYC 3 cut(s) 828, 939, 1186
Eco31I GGTCTC 2 cut(s) 679, 1519
Eco32I GATATC 2 cut(s) 1003, 1576
Eco53kI GAGCTC 2 cut(s) 826, 1184
Eco88I CYCGRG 2 cut(s) 139, 704
Eco91I GGTNACC 1 cut(s) 401
EcoICRI GAGCTC 2 cut(s) 826, 1184
EcoO65I GGTNACC 1 cut(s) 401
EcoRV GATATC 2 cut(s) 1003, 1576
EcoT14I CCWWGG 3 cut(s) 310, 421, 1359
EcoT22I ATGCAT 2 cut(s) 1015, 1262
EcoT38I GRGCYC 3 cut(s) 828, 939, 1186
ErhI CCWWGG 3 cut(s) 310, 421, 1359
FalI AAGNNNNNCTT 6 cut(s) 500, 532, 585, 617, 1352, 1384
FbaI TGATCA 1 cut(s) 106
Fnu4HI GCNGC 4 cut(s) 369, 875, 1293, 1305
FokI GGATG 5 cut(s) 1010, 1097, 1247, 1269, 1445
FriOI GRGCYC 3 cut(s) 828, 939, 1186
Fsp4HI GCNGC 4 cut(s) 369, 875, 1293, 1305
FspBI CTAG 6 cut(s) 65, 230, 590, 680, 857, 1308
GlaI GCGC 3 cut(s) 308, 718, 1122
GluI GCNGC 4 cut(s) 369, 875, 1293, 1305
GsuI CTGGAG 1 cut(s) 644
HaeII RGCGCY 1 cut(s) 310
HaeIII GGCC 2 cut(s) 1238, 1470
HapII CCGG 2 cut(s) 533, 1736
HgaI GACGC 1 cut(s) 1702
HhaI GCGC 3 cut(s) 309, 719, 1123
Hin6I GCGC 3 cut(s) 307, 717, 1121
HinP1I GCGC 3 cut(s) 307, 717, 1121
HindIII AAGCTT 4 cut(s) 514, 544, 750, 1221
HinfI GANTC 4 cut(s) 626, 700, 1364, 1562
HpaII CCGG 2 cut(s) 533, 1736
HphI GGTGA 3 cut(s) 407, 413, 1171
Hpy166II GTNNAC 1 cut(s) 252
Hpy188I TCNGA 5 cut(s) 34, 178, 282, 695, 790
Hpy188III TCNNGA 5 cut(s) 65, 610, 623, 704, 706
Hpy8I GTNNAC 1 cut(s) 252
Hpy99I CGWCG 3 cut(s) 123, 726, 1718
HpyAV CCTTC 4 cut(s) 861, 1156, 1160, 1247
HpyCH4IV ACGT 1 cut(s) 1627
HpyF10VI GCNNNNNNNGC 9 cut(s) 68, 538, 1181, 1190, 1199, 1266, 1301, 1313, 1322
HpyF3I CTNAG 6 cut(s) 99, 437, 542, 827, 1400, 1592
HpySE526I ACGT 1 cut(s) 1627
HspAI GCGC 3 cut(s) 307, 717, 1121
Ksp22I TGATCA 1 cut(s) 106
Kzo9I GATC 6 cut(s) 27, 106, 154, 1038, 1654, 1732
LguI GCTCTTC 1 cut(s) 1165
LmnI GCTCC 1 cut(s) 696
Lsp1109I GCAGC 4 cut(s) 355, 861, 1279, 1291
LweI GCATC 5 cut(s) 286, 1000, 1247, 1269, 1602
MaeI CTAG 6 cut(s) 65, 230, 590, 680, 857, 1308
MaeII ACGT 1 cut(s) 1627
MaeIII GTNAC 3 cut(s) 401, 1354, 1493
MalI GATC 6 cut(s) 29, 108, 156, 1040, 1656, 1734
MbiI CCGCTC 1 cut(s) 691
MboI GATC 6 cut(s) 27, 106, 154, 1038, 1654, 1732
MfeI CAATTG 2 cut(s) 653, 1075
MflI RGATCY 3 cut(s) 27, 1038, 1654
MhlI GDGCHC 3 cut(s) 828, 939, 1186
MlyI GAGTC 3 cut(s) 635, 709, 1373
MmeI TCCRAC 1 cut(s) 931
Mph1103I ATGCAT 2 cut(s) 1015, 1262
MroXI GAANNNNTTC 2 cut(s) 1053, 1133
MseI TTAA 4 cut(s) 518, 773, 1635, 1640
MslI CAYNNNNRTG 3 cut(s) 363, 387, 1744
MspI CCGG 2 cut(s) 533, 1736
MspR9I CCNGG 1 cut(s) 534
MunI CAATTG 2 cut(s) 653, 1075
Mva1269I GAATGC 2 cut(s) 543, 1013
MwoI GCNNNNNNNGC 9 cut(s) 68, 538, 1181, 1190, 1199, 1266, 1301, 1313, 1322
NciI CCSGG 1 cut(s) 534
NdeII GATC 6 cut(s) 27, 106, 154, 1038, 1654, 1732
NheI GCTAGC 1 cut(s) 1307
NlaIV GGNNCC 2 cut(s) 1247, 1740
NmuCI GTSAC 1 cut(s) 401
NsiI ATGCAT 2 cut(s) 1015, 1262
NspI RCATGY 3 cut(s) 1073, 1105, 1691
PaeI GCATGC 1 cut(s) 1073
PaeR7I CTCGAG 1 cut(s) 704
PciSI GCTCTTC 1 cut(s) 1165
PctI GAATGC 2 cut(s) 543, 1013
PdmI GAANNNNTTC 2 cut(s) 1053, 1133
PfeI GAWTC 1 cut(s) 1562
PkrI GCNGC 4 cut(s) 370, 876, 1294, 1306
PleI GAGTC 3 cut(s) 634, 708, 1372
PpsI GAGTC 3 cut(s) 634, 708, 1372
PsiI TTATAA 1 cut(s) 188
Psp124BI GAGCTC 2 cut(s) 828, 1186
PspEI GGTNACC 1 cut(s) 401
PspN4I GGNNCC 2 cut(s) 1247, 1740
PsuI RGATCY 3 cut(s) 27, 1038, 1654
RsaI GTAC 3 cut(s) 51, 323, 1021
RsaNI GTAC 3 cut(s) 50, 322, 1020
RseI CAYNNNNRTG 3 cut(s) 363, 387, 1744
SacI GAGCTC 2 cut(s) 828, 1186
SapI GCTCTTC 1 cut(s) 1165
SaqAI TTAA 4 cut(s) 518, 773, 1635, 1640
SatI GCNGC 4 cut(s) 369, 875, 1293, 1305
Sau3AI GATC 6 cut(s) 27, 106, 154, 1038, 1654, 1732
SchI GAGTC 3 cut(s) 635, 709, 1373
ScrFI CCNGG 1 cut(s) 534
SduI GDGCHC 3 cut(s) 828, 939, 1186
SfaNI GCATC 5 cut(s) 286, 1000, 1247, 1269, 1602
Sfr274I CTCGAG 1 cut(s) 704
SlaI CTCGAG 1 cut(s) 704
SmiMI CAYNNNNRTG 3 cut(s) 363, 387, 1744
SmlI CTYRAG 3 cut(s) 672, 704, 1034
SmoI CTYRAG 3 cut(s) 672, 704, 1034
SphI GCATGC 1 cut(s) 1073
SsiI CCGC 3 cut(s) 136, 689, 899
SspI AATATT 1 cut(s) 471
SspMI CTAG 6 cut(s) 65, 230, 590, 680, 857, 1308
SstI GAGCTC 2 cut(s) 828, 1186
StyD4I CCNGG 1 cut(s) 532
StyI CCWWGG 3 cut(s) 310, 421, 1359
TaiI ACGT 1 cut(s) 1630
TaqI TCGA 3 cut(s) 118, 705, 1445
TatI WGTACW 1 cut(s) 49
TfiI GAWTC 1 cut(s) 1562
Tru1I TTAA 4 cut(s) 518, 773, 1635, 1640
Tru9I TTAA 4 cut(s) 518, 773, 1635, 1640
TscAI CASTG 4 cut(s) 240, 265, 357, 835
TseFI GTSAC 1 cut(s) 401
TseI GCWGC 4 cut(s) 368, 874, 1292, 1304
Tsp45I GTSAC 1 cut(s) 401
TspGWI ACGGA 2 cut(s) 136, 1061
TspRI CASTG 4 cut(s) 240, 265, 357, 835
XapI RAATTY 3 cut(s) 94, 959, 1395
XbaI TCTAGA 1 cut(s) 64
XceI RCATGY 3 cut(s) 1073, 1105, 1691
XcmI CCANNNNNNNNNTGG 2 cut(s) 389, 947
XhoI CTCGAG 1 cut(s) 704
XmnI GAANNNNTTC 2 cut(s) 1053, 1133
XspI CTAG 6 cut(s) 65, 230, 590, 680, 857, 1308
Zsp2I ATGCAT 2 cut(s) 1015, 1262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.