MD11G1236200.v1.1

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000053
Physical Location & Seq
Reverse (-)
34346632 .. 34349128
2497 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1236200.v1.1.491

Sequence Viewer

Length: 1746 bp
ATGATCGTCCACGCATTGTTCTTCAAATTGCCCAAAATTTTCCAACAGGAAAGCATGGAAGACATATCATTAATCCAAGTTTCAGCAGAAGCTCAATCACAAAATCCCAAGCCTGAAGTTGCTCGTCGGACGGCAAATTTTCACCCAAGCATTTGGGGAGATCAGTTCATAAATTATGATGATTCCCAAGACATTATTACTCAAGCTCACAGTCAACAACAGGTTGATGAACTCAAAAAAATTGTGAGGGAAGTATTGATAACCAGTGCAACTGATTTTTCACTTCAGCTAAAGTTGGTTGATGCAATCCAGCGCCTTGGCGTGGCATACCATTTTGAAAGAGAAATCGAGAAAGCACTTGAACGTATGCATGCTACATTTGATGGTGACCATGGCAACGATCATGTTGATGTATACAATGTTGCTCTTGGTTTCCGGCTGCTAAGACAACACGGGTATAATGTTTCATGTGCCATATTCACCAATTTCAAAGATAAAAGTAGTAGCTTCAAGGAAAGTTTGATTGATGACGTGTCAGGTATGCTAAGCTTTTATGAAGCAACACATCTAAGGGTGCATGGAGAAGACATATTAGAAGAGGCTCTTGTATTCACCACCACTCACCTTGGGTCAGCAGCAACCCGTGTAAGCAATCCACTCGCTACACAAATAACTCAAGCCCTAGAGAGACCCCTGCGAAAAAGTCTAGAGAGATTATCTGCGCGACGTTATATTTCAATATACCAAGAACAAGCTTCACATAATGTCTCTCTTCTGAAACTTGCAAAGTTAGACTTCAATCTTGTTCAGCTTTTGCACAAAAAGGAGCTCCAAGAGATTACTAGGTGGTGGAGAGCACTAGATTTTGAAGGGAAGCTGCCATTTGCAAGAGATAGGATGGTAGAGTTGTACTTTTGGATCGTCGGAGTGTATTTTGAACCCCGGTACTCTGTTGGGAGAAAAATTATGACAAAAGCAAGTGTCTTGCTGACAATATTGGATGATATCTACGATGCATTTGGTACATTTGAAGAGCTCGTAGTCTTTACTGAAGCGATTGACAGGTGGGATGTCAATTGCATTGATGAACTCCCGGAATATATGCAAACATTTTATCGTGCACTTTTAAATCTCTACAATGATATTGAGGCAGAGATGGCGAAGGAAGGAAGATCATACCGAGTTCCATATGCAATACAAGCTATGAAAGATCAAGCTCGATCTTATTTTAATGAGGCCCGATGGTTGCACGAGGGACGAGTTCCAAGTATGGAGGAGTATATGCGCGTTGCAACAGTTTCGATTAGTTACACCTTTCTTACAACCATATATTTACTTGGCATGGGAGATGTTGTGACAAAGGAAGCATTTGAGTGGTTGTTCACTGACCCCAAAATTGTTAGAGCTGCAAATACCATTTTTAGGCTCATGAATGACATTGTTTCGACAAACTTTGAGAAAGAGAGAGGGCATGCTGCTTCTAGTGTTGATTGCTACATGAAGCAATATGGGGTGTCAGAGCAAGAGACAGTTGATGTTTTTAAGAAACAAATTATGGATTTGTGGAAGGATATAAATGCGGAGTTCTTTAGACCAACTTCTGTGTCAATGCATGTTCTTATGCGCGTTCTCAATTTAACAAGAGTAGTTGATCTTCTTTACAAAAAGGAAGATGGATTCACACATGTGGGGAAGGTGATGAAAGATAGTGTTGCTTCAATTTGTATCGATCCATTGCCACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

582

Amino Acids

67.25

Weight (kDa)

5.65

Isoelectric Point (pI)

34.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 51 - 228 1.7e-56 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 287 - 524 4.9e-98 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 327 - 524 8.7e-62 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 414
AccII CGCG 3 cut(s) 724, 1287, 1626
AciI CCGC 1 cut(s) 1580
AclWI GGATC 2 cut(s) 926, 1724
AcsI RAATTY 2 cut(s) 36, 136
AcuI CTGAAG 3 cut(s) 135, 269, 1071
AfaI GTAC 3 cut(s) 911, 947, 1024
AfiI CCNNNNNNNGG 2 cut(s) 322, 1422
AflIII ACRYGT 2 cut(s) 531, 1684
AjiI CACGTC 1 cut(s) 532
AleI CACNNNNGTG 1 cut(s) 1685
Alw21I GWGCWC 4 cut(s) 831, 859, 1038, 1123
Alw26I GTCTC 3 cut(s) 682, 772, 1520
Alw44I GTGCAC 1 cut(s) 1119
AlwI GGATC 2 cut(s) 926, 1724
AoxI GGCC 1 cut(s) 1236
ApaLI GTGCAC 1 cut(s) 1119
ApeKI GCWGC 5 cut(s) 439, 635, 877, 1406, 1475
ApoI RAATTY 2 cut(s) 36, 136
AseI ATTAAT 1 cut(s) 71
AspLEI GCGC 4 cut(s) 315, 724, 1287, 1626
AspS9I GGNCC 1 cut(s) 1237
AsuC2I CCSGG 2 cut(s) 943, 1094
AsuHPI GGTGA 6 cut(s) 134, 398, 472, 604, 614, 1708
BaeGI GKGCMC 1 cut(s) 1123
BanII GRGCYC 2 cut(s) 831, 1038
BarI GAAGNNNNNNTAC 2 cut(s) 1638, 1670
BauI CACGAG 1 cut(s) 1250
BbsI GAAGAC 2 cut(s) 66, 591
Bbv12I GWGCWC 4 cut(s) 831, 859, 1038, 1123
BbvI GCAGC 5 cut(s) 426, 647, 864, 1393, 1462
BccI CCATC 5 cut(s) 377, 892, 1150, 1236, 1667
BceAI ACGGC 1 cut(s) 147
BcgI CGANNNNNNTGC 4 cut(s) 640, 674, 1281, 1315
BcnI CCSGG 2 cut(s) 943, 1094
BcoDI GTCTC 3 cut(s) 682, 772, 1520
BfaI CTAG 5 cut(s) 683, 707, 843, 860, 1482
BfoI RGCGCY 1 cut(s) 316
BisI GCNGC 5 cut(s) 440, 636, 878, 1407, 1476
BlpI GCTNAGC 1 cut(s) 545
BlsI GCNGC 5 cut(s) 441, 637, 879, 1408, 1477
Bme1390I CCNGG 2 cut(s) 943, 1094
BmgBI CACGTC 1 cut(s) 532
BmgT120I GGNCC 1 cut(s) 1237
BmrFI CCNGG 2 cut(s) 943, 1094
BmsI GCATC 2 cut(s) 292, 1003
BpiI GAAGAC 2 cut(s) 66, 591
Bpu1102I GCTNAGC 1 cut(s) 545
BpuEI CTTGAG 2 cut(s) 186, 660
BpuMI CCSGG 2 cut(s) 943, 1094
Bsa29I ATCGAT 1 cut(s) 1728
BsaI GGTCTC 1 cut(s) 682
BsaJI CCNNGG 4 cut(s) 316, 391, 625, 941
BsaXI ACNNNNNCTCC 2 cut(s) 1338, 1368
Bsc4I CCNNNNNNNGG 2 cut(s) 322, 1422
Bse1I ACTGG 1 cut(s) 264
Bse3DI GCAATG 1 cut(s) 1733
BseCI ATCGAT 1 cut(s) 1728
BseDI CCNNGG 4 cut(s) 316, 391, 625, 941
BseGI GGATG 3 cut(s) 903, 1006, 1075
BseLI CCNNNNNNNGG 2 cut(s) 322, 1422
BseMI GCAATG 1 cut(s) 1733
BseNI ACTGG 1 cut(s) 264
BseRI GAGGAG 1 cut(s) 1289
BseSI GKGCMC 1 cut(s) 1123
BseXI GCAGC 5 cut(s) 426, 647, 864, 1393, 1462
Bsh1236I CGCG 3 cut(s) 724, 1287, 1626
BshFI GGCC 1 cut(s) 1238
BshVI ATCGAT 1 cut(s) 1728
BsiHKAI GWGCWC 4 cut(s) 831, 859, 1038, 1123
BsiSI CCGG 3 cut(s) 436, 943, 1094
BslFI GGGAC 1 cut(s) 1269
BslI CCNNNNNNNGG 2 cut(s) 322, 1422
BsmAI GTCTC 3 cut(s) 682, 772, 1520
BsmFI GGGAC 1 cut(s) 1269
BsnI GGCC 1 cut(s) 1238
Bso31I GGTCTC 1 cut(s) 682
Bsp1286I GDGCHC 4 cut(s) 831, 859, 1038, 1123
Bsp143I GATC 9 cut(s) 3, 160, 400, 918, 1172, 1210, 1220, 1651, 1729
Bsp1720I GCTNAGC 1 cut(s) 545
Bsp19I CCATGG 1 cut(s) 391
BspACI CCGC 1 cut(s) 1580
BspANI GGCC 1 cut(s) 1238
BspDI ATCGAT 1 cut(s) 1728
BspFNI CGCG 3 cut(s) 724, 1287, 1626
BspHI TCATGA 1 cut(s) 1428
BspPI GGATC 2 cut(s) 926, 1724
BspQI GCTCTTC 1 cut(s) 1026
BspTNI GGTCTC 1 cut(s) 682
BsrDI GCAATG 1 cut(s) 1733
BsrI ACTGG 1 cut(s) 264
BssECI CCNNGG 4 cut(s) 316, 391, 625, 941
BssMI GATC 9 cut(s) 3, 160, 400, 918, 1172, 1210, 1220, 1651, 1729
BssNAI GTATAC 1 cut(s) 415
BssSI CACGAG 1 cut(s) 1250
BssT1I CCWWGG 3 cut(s) 316, 391, 625
Bst1107I GTATAC 1 cut(s) 415
Bst2BI CACGAG 1 cut(s) 1250
Bst4CI ACNGT 3 cut(s) 212, 1297, 1531
Bst6I CTCTTC 3 cut(s) 591, 777, 1026
BstC8I GCNNGC 2 cut(s) 372, 1473
BstDEI CTNAG 3 cut(s) 443, 545, 569
BstDSI CCRYGG 1 cut(s) 391
BstEII GGTNACC 1 cut(s) 386
BstF5I GGATG 3 cut(s) 903, 1006, 1075
BstFNI CGCG 3 cut(s) 724, 1287, 1626
BstH2I RGCGCY 1 cut(s) 316
BstHHI GCGC 4 cut(s) 315, 724, 1287, 1626
BstKTI GATC 9 cut(s) 6, 163, 403, 921, 1175, 1213, 1223, 1654, 1732
BstMAI GTCTC 3 cut(s) 682, 772, 1520
BstMBI GATC 9 cut(s) 3, 160, 400, 918, 1172, 1210, 1220, 1651, 1729
BstMWI GCNNNNNNNGC 2 cut(s) 1157, 1199
BstNSI RCATGY 4 cut(s) 374, 1475, 1616, 1688
BstPI GGTNACC 1 cut(s) 386
BstSCI CCNGG 2 cut(s) 941, 1092
BstSLI GKGCMC 1 cut(s) 1123
BstUI CGCG 3 cut(s) 724, 1287, 1626
BstV1I GCAGC 5 cut(s) 426, 647, 864, 1393, 1462
BstV2I GAAGAC 2 cut(s) 66, 591
BstXI CCANNNNNNTGG 2 cut(s) 153, 317
BstZ17I GTATAC 1 cut(s) 415
Bsu15I ATCGAT 1 cut(s) 1728
BsuRI GGCC 1 cut(s) 1238
BsuTUI ATCGAT 1 cut(s) 1728
BtgI CCRYGG 1 cut(s) 391
BtrI CACGTC 1 cut(s) 532
BtsCI GGATG 3 cut(s) 903, 1006, 1075
BtsIMutI CAGTG 2 cut(s) 271, 1383
Cac8I GCNNGC 2 cut(s) 372, 1473
CciI TCATGA 1 cut(s) 1428
CfoI GCGC 4 cut(s) 315, 724, 1287, 1626
Cfr13I GGNCC 1 cut(s) 1237
ClaI ATCGAT 1 cut(s) 1728
Csp6I GTAC 3 cut(s) 910, 946, 1023
CspCI CAANNNNNGTGG 2 cut(s) 607, 642
CviQI GTAC 3 cut(s) 910, 946, 1023
DdeI CTNAG 3 cut(s) 443, 545, 569
DpnI GATC 9 cut(s) 5, 162, 402, 920, 1174, 1212, 1222, 1653, 1731
DpnII GATC 9 cut(s) 3, 160, 400, 918, 1172, 1210, 1220, 1651, 1729
DraI TTTAAA 1 cut(s) 1128
Eam1104I CTCTTC 3 cut(s) 591, 777, 1026
EarI CTCTTC 3 cut(s) 591, 777, 1026
Ecl136II GAGCTC 2 cut(s) 829, 1036
Eco130I CCWWGG 3 cut(s) 316, 391, 625
Eco24I GRGCYC 2 cut(s) 831, 1038
Eco31I GGTCTC 1 cut(s) 682
Eco32I GATATC 1 cut(s) 1006
Eco53kI GAGCTC 2 cut(s) 829, 1036
Eco57I CTGAAG 3 cut(s) 135, 269, 1071
Eco91I GGTNACC 1 cut(s) 386
EcoICRI GAGCTC 2 cut(s) 829, 1036
EcoO65I GGTNACC 1 cut(s) 386
EcoRV GATATC 1 cut(s) 1006
EcoT14I CCWWGG 3 cut(s) 316, 391, 625
EcoT22I ATGCAT 3 cut(s) 372, 1018, 1614
EcoT38I GRGCYC 2 cut(s) 831, 1038
ErhI CCWWGG 3 cut(s) 316, 391, 625
FalI AAGNNNNNCTT 4 cut(s) 588, 620, 779, 811
FaqI GGGAC 1 cut(s) 1269
FauNDI CATATG 1 cut(s) 1189
FblI GTMKAC 1 cut(s) 414
Fnu4HI GCNGC 5 cut(s) 440, 636, 878, 1407, 1476
FokI GGATG 3 cut(s) 910, 1013, 1082
FriOI GRGCYC 2 cut(s) 831, 1038
Fsp4HI GCNGC 5 cut(s) 440, 636, 878, 1407, 1476
FspBI CTAG 5 cut(s) 683, 707, 843, 860, 1482
GlaI GCGC 4 cut(s) 314, 723, 1286, 1625
GluI GCNGC 5 cut(s) 440, 636, 878, 1407, 1476
HaeII RGCGCY 1 cut(s) 316
HaeIII GGCC 1 cut(s) 1238
HapII CCGG 3 cut(s) 436, 943, 1094
HhaI GCGC 4 cut(s) 315, 724, 1287, 1626
Hin6I GCGC 4 cut(s) 313, 722, 1285, 1624
HinP1I GCGC 4 cut(s) 313, 722, 1285, 1624
HincII GTYRAC 1 cut(s) 215
HindII GTYRAC 1 cut(s) 215
HindIII AAGCTT 2 cut(s) 547, 753
HinfI GANTC 2 cut(s) 182, 1677
HpaII CCGG 3 cut(s) 436, 943, 1094
HphI GGTGA 6 cut(s) 134, 398, 472, 604, 614, 1708
Hpy166II GTNNAC 5 cut(s) 10, 215, 415, 1121, 1383
Hpy188I TCNGA 4 cut(s) 129, 777, 926, 1519
Hpy188III TCNNGA 3 cut(s) 349, 707, 1429
Hpy8I GTNNAC 5 cut(s) 10, 215, 415, 1121, 1383
Hpy99I CGWCG 3 cut(s) 129, 729, 926
HpyAV CCTTC 5 cut(s) 863, 1156, 1160, 1561, 1687
HpyCH4III ACNGT 3 cut(s) 212, 1297, 1531
HpyCH4IV ACGT 3 cut(s) 364, 531, 727
HpyF10VI GCNNNNNNNGC 2 cut(s) 1157, 1199
HpyF3I CTNAG 3 cut(s) 443, 545, 569
HpySE526I ACGT 3 cut(s) 364, 531, 727
HspAI GCGC 4 cut(s) 313, 722, 1285, 1624
Kzo9I GATC 9 cut(s) 3, 160, 400, 918, 1172, 1210, 1220, 1651, 1729
LguI GCTCTTC 1 cut(s) 1026
LmnI GCTCC 2 cut(s) 826, 834
Lsp1109I GCAGC 5 cut(s) 426, 647, 864, 1393, 1462
LweI GCATC 2 cut(s) 292, 1003
MaeI CTAG 5 cut(s) 683, 707, 843, 860, 1482
MaeII ACGT 3 cut(s) 364, 531, 727
MaeIII GTNAC 3 cut(s) 386, 1307, 1354
MalI GATC 9 cut(s) 5, 162, 402, 920, 1174, 1212, 1222, 1653, 1731
MboI GATC 9 cut(s) 3, 160, 400, 918, 1172, 1210, 1220, 1651, 1729
MboII GAAGA 9 cut(s) 13, 71, 596, 608, 764, 1043, 1182, 1646, 1682
MfeI CAATTG 1 cut(s) 1075
MhlI GDGCHC 4 cut(s) 831, 859, 1038, 1123
MmeI TCCRAC 3 cut(s) 67, 107, 904
MnlI CCTC 7 cut(s) 240, 592, 1141, 1228, 1246, 1267, 1460
Mph1103I ATGCAT 3 cut(s) 372, 1018, 1614
MseI TTAA 5 cut(s) 71, 1127, 1230, 1542, 1637
MslI CAYNNNNRTG 3 cut(s) 408, 1372, 1685
MspI CCGG 3 cut(s) 436, 943, 1094
MspR9I CCNGG 2 cut(s) 943, 1094
MunI CAATTG 1 cut(s) 1075
MvnI CGCG 3 cut(s) 724, 1287, 1626
MwoI GCNNNNNNNGC 2 cut(s) 1157, 1199
NciI CCSGG 2 cut(s) 943, 1094
NcoI CCATGG 1 cut(s) 391
NdeI CATATG 1 cut(s) 1189
NdeII GATC 9 cut(s) 3, 160, 400, 918, 1172, 1210, 1220, 1651, 1729
NmuCI GTSAC 2 cut(s) 386, 1354
NsiI ATGCAT 3 cut(s) 372, 1018, 1614
NspI RCATGY 4 cut(s) 374, 1475, 1616, 1688
OliI CACNNNNGTG 1 cut(s) 1685
PaeI GCATGC 2 cut(s) 374, 1475
PagI TCATGA 1 cut(s) 1428
PciI ACATGT 1 cut(s) 1684
PciSI GCTCTTC 1 cut(s) 1026
PfeI GAWTC 2 cut(s) 182, 1677
PfoI TCCNGGA 1 cut(s) 1092
PkrI GCNGC 5 cut(s) 441, 637, 879, 1408, 1477
PscI ACATGT 1 cut(s) 1684
PshBI ATTAAT 1 cut(s) 71
Psp124BI GAGCTC 2 cut(s) 831, 1038
PspEI GGTNACC 1 cut(s) 386
PspPI GGNCC 1 cut(s) 1237
RsaI GTAC 3 cut(s) 911, 947, 1024
RsaNI GTAC 3 cut(s) 910, 946, 1023
RseI CAYNNNNRTG 3 cut(s) 408, 1372, 1685
SacI GAGCTC 2 cut(s) 831, 1038
SapI GCTCTTC 1 cut(s) 1026
SaqAI TTAA 5 cut(s) 71, 1127, 1230, 1542, 1637
SatI GCNGC 5 cut(s) 440, 636, 878, 1407, 1476
Sau3AI GATC 9 cut(s) 3, 160, 400, 918, 1172, 1210, 1220, 1651, 1729
Sau96I GGNCC 1 cut(s) 1237
ScrFI CCNGG 2 cut(s) 943, 1094
SduI GDGCHC 4 cut(s) 831, 859, 1038, 1123
SfaNI GCATC 2 cut(s) 292, 1003
SmiMI CAYNNNNRTG 3 cut(s) 408, 1372, 1685
SmlI CTYRAG 2 cut(s) 201, 675
SmoI CTYRAG 2 cut(s) 201, 675
SphI GCATGC 2 cut(s) 374, 1475
SsiI CCGC 1 cut(s) 1580
SspI AATATT 1 cut(s) 996
SspMI CTAG 5 cut(s) 683, 707, 843, 860, 1482
SstI GAGCTC 2 cut(s) 831, 1038
StyD4I CCNGG 2 cut(s) 941, 1092
StyI CCWWGG 3 cut(s) 316, 391, 625
TaaI ACNGT 3 cut(s) 212, 1297, 1531
TaiI ACGT 3 cut(s) 367, 534, 730
TaqI TCGA 5 cut(s) 348, 1219, 1301, 1445, 1728
TatI WGTACW 1 cut(s) 909
TfiI GAWTC 2 cut(s) 182, 1677
Tru1I TTAA 5 cut(s) 71, 1127, 1230, 1542, 1637
Tru9I TTAA 5 cut(s) 71, 1127, 1230, 1542, 1637
TscAI CASTG 2 cut(s) 271, 1390
TseFI GTSAC 2 cut(s) 386, 1354
TseI GCWGC 5 cut(s) 439, 635, 877, 1406, 1475
Tsp45I GTSAC 2 cut(s) 386, 1354
TspDTI ATGAA 9 cut(s) 157, 243, 456, 570, 1101, 1220, 1445, 1514, 1715
TspRI CASTG 2 cut(s) 271, 1390
VneI GTGCAC 1 cut(s) 1119
VspI ATTAAT 1 cut(s) 71
XapI RAATTY 2 cut(s) 36, 136
XbaI TCTAGA 1 cut(s) 706
XceI RCATGY 4 cut(s) 374, 1475, 1616, 1688
XmiI GTMKAC 1 cut(s) 414
XspI CTAG 5 cut(s) 683, 707, 843, 860, 1482
Zsp2I ATGCAT 3 cut(s) 372, 1018, 1614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.