FvH4_1g04000

(-)-germacrene D synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000053
Physical Location & Seq
Forward (+)
2124380 .. 2127362
2983 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g04000.t1

Sequence Viewer

Length: 1683 bp
ATGTCTCTCACCTCCGTCATTTTAGCTTCCCAATCCCCTCAAACCCTGAATAATGCCGATACTCGACGTTCGGCTAAATACCATCCCAGTATTTGGGGAGATCGGTTTCTGTCTTATAACAACACTATGGAAACTAACATGCAAGGTAAGCAACAAGTAATCCAACAACTGAAGGAAGAAGTGAAGAGGATGCTGATGGCTCCGCCGGTTGAAACTGGTTTGGGAAAACTGGAGTTGATTGATGACATTCAACGCTTAGGCGTGTCTTACCACTTCGAATACGAGATTGACCAAACTATGCAGCAAATTCACGAAAACCTTAATGGCACCTATGATGATGATGACTTGCACACTTGTGCTCTTCGTTTTCGCTTGCTAAGGCAGCATGGTTATAATGTTTCTTGTGATATGTTCAACAAGTTCAAAGATTGTGATGGGAAATTCGACGAATCGCTTCACCACGATGTAGCAGGACTCCAAAGCTTGTACGAAGCCACACATCTTAGGGTGCGAGGAGAGGATTTTCTAGAAGAAGCACTAGCCTTCACAACCACACATCTACAGTCGGCAGCAAATCGTTTGAGCCCCCCGCTTTCGAAACAAGTAAGGCATGCCTTGAATCAGCCACTTCGGAAGGGCTTACCGAGGCTAGAAGCGAGGCACTACATGTCTCTTCACCAGGAACTGCATGGATCACGTAACCATGTTTTGCTGAGATTTGCAAAGCTGGATTTCAACCTGCTCCAACAAGTGCATCAGAAAGAACTAAGTGATATTGCAAGGTGGTGGAAGAAGTTGGACTTTGCTAGCAAGCTTCCTTTTGCAAGAGACAGAGTGATTGAGTGCTACTTCTGGATTTTGGGTGTCTACTTTGAACCCAAGTATTACTTTGCTAGGAGAACCCTTACCAAAGTTATTGCCATGACATCCATTATTGATGACATTTACGATGTGTATGGCACTCTTGAAGAACTAGATCTCTTTACTAGAGCAATTGAGAGATGGGACATCTCAGCCATGGATGTACTGCCGGAGTATATGAAATTCTGTTACGAGGCACTCTTAGATGTTTATGCTGAAGCAGAACAGGGGCTTGCAAGCGAGGGAAAATCATACCGTATCGATTATGCAAAAGAAGCGATGAAGAGACAAGTTAGGGCATACCATGCTGAAGCCAAATGGTTCCACAACAACTACACACCAACAATGGACGAATACATGGAAGTAGCCCTCGTCACTTCAGCCTACTCAATGCTAGCAACCACATCCTTTGTTGGAATGGGAGATATTGTTACTAAAGACTCTTTCGAGTGGATCTTCAGTGACCCTAAGATGGTAAAGGCTTCAGCAGTAGTTTGCAGACTCATGGATGACATTGTATCCCACAAGTTTGAGCAAAAGAGAGGACATGTTGCTTCGGCCGTGGAATGCTACATGATACAATACGGTGCGACAGAAGAAGAAACCATAATCGAATTTCGAAAACAAGTGAGTGATGCATGGAAGGACATAAATGAAGAGTGCCTCCACCCTACCTCTGTCAACATGCCTCTCTTGATGCGAGTTCTGAATCTCACACGCGTGATAGATGTACTGTACAAGTCTGAAGATGGCTACACTCATGCTGGAACCATACTCAAAGATTTTGTAGCATCTCTACTGGTAGATCCTGTGACTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

561

Amino Acids

64.89

Weight (kDa)

5.74

Isoelectric Point (pI)

48.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 31 - 206 5.7e-61 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 264 - 503 1.2e-104 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 304 - 503 4e-62 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 117, 393
Acc36I ACCTGC 1 cut(s) 747
AccB1I GGYRCC 1 cut(s) 326
AccB7I CCANNNNNTGG 1 cut(s) 93
AccI GTMKAC 1 cut(s) 867
AccII CGCG 1 cut(s) 1581
AciI CCGC 2 cut(s) 203, 590
AclWI GGATC 3 cut(s) 700, 1322, 1661
AcoI YGGCCR 1 cut(s) 1419
AcsI RAATTY 4 cut(s) 306, 440, 1043, 1475
AcuI CTGAAG 7 cut(s) 191, 1098, 1191, 1224, 1303, 1329, 1626
AfaI GTAC 4 cut(s) 488, 1026, 1593, 1598
AfiI CCNNNNNNNGG 2 cut(s) 93, 1333
AflIII ACRYGT 3 cut(s) 666, 1408, 1579
AgsI TTSAA 8 cut(s) 212, 251, 415, 424, 619, 736, 875, 968
AjnI CCWGG 1 cut(s) 678
AleI CACNNNNGTG 2 cut(s) 354, 1580
AluBI AGCT 4 cut(s) 26, 483, 727, 814
AluI AGCT 4 cut(s) 26, 483, 727, 814
Alw21I GWGCWC 1 cut(s) 361
Alw26I GTCTC 4 cut(s) 9, 675, 822, 1141
AlwI GGATC 3 cut(s) 700, 1322, 1661
AlwNI CAGNNNCTG 1 cut(s) 685
AoxI GGCC 1 cut(s) 1419
ApeKI GCWGC 3 cut(s) 301, 382, 569
ApoI RAATTY 4 cut(s) 306, 440, 1043, 1475
Asp700I GAANNNNTTC 1 cut(s) 453
AsuHPI GGTGA 2 cut(s) 449, 668
AsuII TTCGAA 3 cut(s) 276, 596, 1480
AsuNHI GCTAGC 2 cut(s) 806, 1255
BanI GGYRCC 1 cut(s) 326
BanII GRGCYC 1 cut(s) 587
Bbv12I GWGCWC 1 cut(s) 361
BbvI GCAGC 3 cut(s) 313, 394, 581
BccI CCATC 6 cut(s) 90, 190, 428, 996, 1327, 1604
BceAI ACGGC 1 cut(s) 1406
BciT130I CCWGG 1 cut(s) 680
BciVI GTATCC 1 cut(s) 1390
BcoDI GTCTC 4 cut(s) 9, 675, 822, 1141
BfaI CTAG 8 cut(s) 527, 539, 650, 807, 894, 974, 987, 1256
BfmI CTRYAG 1 cut(s) 560
BfuAI ACCTGC 1 cut(s) 747
BfuI GTATCC 1 cut(s) 1390
BglII AGATCT 1 cut(s) 976
BisI GCNGC 3 cut(s) 302, 383, 570
BlsI GCNGC 3 cut(s) 303, 384, 571
Bme1390I CCNGG 1 cut(s) 680
BmiI GGNNCC 4 cut(s) 201, 328, 1184, 1630
BmrFI CCNGG 1 cut(s) 680
BmrI ACTGGG 1 cut(s) 81
BmsI GCATC 5 cut(s) 180, 763, 1486, 1548, 1661
BmtI GCTAGC 2 cut(s) 810, 1259
BmuI ACTGGG 1 cut(s) 81
BpmI CTGGAG 1 cut(s) 251
Bpu10I CCTNAGC 2 cut(s) 256, 377
Bpu14I TTCGAA 3 cut(s) 276, 596, 1480
Bsa29I ATCGAT 1 cut(s) 1122
BsaAI YACGTR 1 cut(s) 698
BsaJI CCNNGG 3 cut(s) 644, 1017, 1422
BsaXI ACNNNNNCTCC 2 cut(s) 1275, 1305
Bsc4I CCNNNNNNNGG 2 cut(s) 93, 1333
Bse118I RCCGGY 1 cut(s) 205
Bse1I ACTGG 4 cut(s) 87, 220, 234, 1665
BseBI CCWGG 1 cut(s) 680
BseCI ATCGAT 1 cut(s) 1122
BseDI CCNNGG 3 cut(s) 644, 1017, 1422
BseGI GGATG 6 cut(s) 82, 195, 926, 1027, 1265, 1375
BseLI CCNNNNNNNGG 2 cut(s) 93, 1333
BseMII CTCAG 2 cut(s) 704, 1026
BseNI ACTGG 4 cut(s) 87, 220, 234, 1665
BseRI GAGGAG 1 cut(s) 528
BseX3I CGGCCG 1 cut(s) 1419
BseXI GCAGC 3 cut(s) 313, 394, 581
Bsh1236I CGCG 1 cut(s) 1581
Bsh1285I CGRYCG 1 cut(s) 1422
BshFI GGCC 1 cut(s) 1421
BshNI GGYRCC 1 cut(s) 326
BshVI ATCGAT 1 cut(s) 1122
BsiEI CGRYCG 1 cut(s) 1422
BsiHKAI GWGCWC 1 cut(s) 361
BsiSI CCGG 2 cut(s) 206, 1031
BslFI GGGAC 1 cut(s) 1019
BslI CCNNNNNNNGG 2 cut(s) 93, 1333
BsmAI GTCTC 4 cut(s) 9, 675, 822, 1141
BsmFI GGGAC 1 cut(s) 1019
BsmI GAATGC 1 cut(s) 1433
BsnI GGCC 1 cut(s) 1421
Bsp119I TTCGAA 3 cut(s) 276, 596, 1480
Bsp1286I GDGCHC 2 cut(s) 361, 587
Bsp1407I TGTACA 1 cut(s) 1596
Bsp143I GATC 5 cut(s) 100, 692, 976, 1314, 1666
Bsp19I CCATGG 1 cut(s) 1017
BspACI CCGC 2 cut(s) 203, 590
BspANI GGCC 1 cut(s) 1421
BspCNI CTCAG 2 cut(s) 705, 1025
BspDI ATCGAT 1 cut(s) 1122
BspFNI CGCG 1 cut(s) 1581
BspLI GGNNCC 4 cut(s) 201, 328, 1184, 1630
BspMI ACCTGC 1 cut(s) 747
BspOI GCTAGC 2 cut(s) 810, 1259
BspPI GGATC 3 cut(s) 700, 1322, 1661
BspQI GCTCTTC 1 cut(s) 366
BspT104I TTCGAA 3 cut(s) 276, 596, 1480
BspT107I GGYRCC 1 cut(s) 326
BsrFI RCCGGY 1 cut(s) 205
BsrGI TGTACA 1 cut(s) 1596
BsrI ACTGG 4 cut(s) 87, 220, 234, 1665
BssAI RCCGGY 1 cut(s) 205
BssECI CCNNGG 3 cut(s) 644, 1017, 1422
BssMI GATC 5 cut(s) 100, 692, 976, 1314, 1666
BssT1I CCWWGG 1 cut(s) 1017
Bst2UI CCWGG 1 cut(s) 680
Bst4CI ACNGT 5 cut(s) 564, 1118, 1448, 1596, 1678
Bst6I CTCTTC 5 cut(s) 179, 366, 678, 1139, 1512
BstAPI GCANNNNNTGC 1 cut(s) 1166
BstAUI TGTACA 1 cut(s) 1596
BstBAI YACGTR 1 cut(s) 698
BstBI TTCGAA 3 cut(s) 276, 596, 1480
BstC8I GCNNGC 7 cut(s) 374, 612, 808, 812, 1095, 1099, 1257
BstDEI CTNAG 8 cut(s) 256, 377, 503, 713, 767, 1012, 1063, 1329
BstDSI CCRYGG 2 cut(s) 1017, 1422
BstF5I GGATG 6 cut(s) 82, 195, 926, 1027, 1265, 1375
BstFNI CGCG 1 cut(s) 1581
BstKTI GATC 5 cut(s) 103, 695, 979, 1317, 1669
BstMAI GTCTC 4 cut(s) 9, 675, 822, 1141
BstMBI GATC 5 cut(s) 100, 692, 976, 1314, 1666
BstMCI CGRYCG 1 cut(s) 1422
BstMWI GCNNNNNNNGC 4 cut(s) 148, 382, 1136, 1166
BstNI CCWGG 1 cut(s) 680
BstNSI RCATGY 5 cut(s) 142, 614, 670, 1412, 1549
BstSCI CCNGG 1 cut(s) 678
BstSFI CTRYAG 1 cut(s) 560
BstUI CGCG 1 cut(s) 1581
BstV1I GCAGC 3 cut(s) 313, 394, 581
BstX2I RGATCY 3 cut(s) 976, 1314, 1666
BstYI RGATCY 3 cut(s) 976, 1314, 1666
BstZI CGGCCG 1 cut(s) 1419
Bsu15I ATCGAT 1 cut(s) 1122
BsuI GTATCC 1 cut(s) 1390
BsuRI GGCC 1 cut(s) 1421
BsuTUI ATCGAT 1 cut(s) 1122
BtgI CCRYGG 2 cut(s) 1017, 1422
BtgZI GCGATG 1 cut(s) 1154
BtsCI GGATG 6 cut(s) 82, 195, 926, 1027, 1265, 1375
BtsIMutI CAGTG 1 cut(s) 1327
BveI ACCTGC 1 cut(s) 747
Cac8I GCNNGC 7 cut(s) 374, 612, 808, 812, 1095, 1099, 1257
CaiI CAGNNNCTG 1 cut(s) 685
Cfr10I RCCGGY 1 cut(s) 205
ClaI ATCGAT 1 cut(s) 1122
Csp6I GTAC 4 cut(s) 487, 1025, 1592, 1597
CspCI CAANNNNNGTGG 2 cut(s) 1252, 1287
CviQI GTAC 4 cut(s) 487, 1025, 1592, 1597
DdeI CTNAG 8 cut(s) 256, 377, 503, 713, 767, 1012, 1063, 1329
DpnI GATC 5 cut(s) 102, 694, 978, 1316, 1668
DpnII GATC 5 cut(s) 100, 692, 976, 1314, 1666
EaeI YGGCCR 1 cut(s) 1419
EagI CGGCCG 1 cut(s) 1419
Eam1104I CTCTTC 5 cut(s) 179, 366, 678, 1139, 1512
EarI CTCTTC 5 cut(s) 179, 366, 678, 1139, 1512
EciI GGCGGA 1 cut(s) 192
EclXI CGGCCG 1 cut(s) 1419
Eco130I CCWWGG 1 cut(s) 1017
Eco24I GRGCYC 1 cut(s) 587
Eco52I CGGCCG 1 cut(s) 1419
Eco57I CTGAAG 7 cut(s) 191, 1098, 1191, 1224, 1303, 1329, 1626
EcoRII CCWGG 1 cut(s) 678
EcoT14I CCWWGG 1 cut(s) 1017
EcoT22I ATGCAT 1 cut(s) 1501
EcoT38I GRGCYC 1 cut(s) 587
ErhI CCWWGG 1 cut(s) 1017
FalI AAGNNNNNCTT 4 cut(s) 785, 817, 872, 904
FaqI GGGAC 1 cut(s) 1019
FauI CCCGC 1 cut(s) 597
FblI GTMKAC 1 cut(s) 867
Fnu4HI GCNGC 3 cut(s) 302, 383, 570
FokI GGATG 6 cut(s) 69, 202, 913, 1034, 1252, 1382
FriOI GRGCYC 1 cut(s) 587
Fsp4HI GCNGC 3 cut(s) 302, 383, 570
FspBI CTAG 8 cut(s) 527, 539, 650, 807, 894, 974, 987, 1256
GluI GCNGC 3 cut(s) 302, 383, 570
GsuI CTGGAG 1 cut(s) 251
HaeIII GGCC 1 cut(s) 1421
HapII CCGG 2 cut(s) 206, 1031
HincII GTYRAC 1 cut(s) 1543
HindII GTYRAC 1 cut(s) 1543
HindIII AAGCTT 2 cut(s) 481, 812
HinfI GANTC 6 cut(s) 449, 474, 619, 1301, 1362, 1570
HpaII CCGG 2 cut(s) 206, 1031
HphI GGTGA 2 cut(s) 449, 668
Hpy166II GTNNAC 2 cut(s) 868, 1543
Hpy188I TCNGA 4 cut(s) 633, 759, 1569, 1606
Hpy188III TCNNGA 5 cut(s) 311, 527, 853, 965, 1555
Hpy8I GTNNAC 2 cut(s) 868, 1543
Hpy99I CGWCG 2 cut(s) 69, 449
HpyAV CCTTC 4 cut(s) 166, 553, 628, 1498
HpyCH4III ACNGT 5 cut(s) 564, 1118, 1448, 1596, 1678
HpyCH4IV ACGT 2 cut(s) 67, 697
HpyF10VI GCNNNNNNNGC 4 cut(s) 148, 382, 1136, 1166
HpyF3I CTNAG 8 cut(s) 256, 377, 503, 713, 767, 1012, 1063, 1329
HpySE526I ACGT 2 cut(s) 67, 697
Kzo9I GATC 5 cut(s) 100, 692, 976, 1314, 1666
LguI GCTCTTC 1 cut(s) 366
LmnI GCTCC 2 cut(s) 205, 747
Lsp1109I GCAGC 3 cut(s) 313, 394, 581
LweI GCATC 5 cut(s) 180, 763, 1486, 1548, 1661
MaeI CTAG 8 cut(s) 527, 539, 650, 807, 894, 974, 987, 1256
MaeII ACGT 2 cut(s) 67, 697
MaeIII GTNAC 6 cut(s) 698, 1049, 1234, 1291, 1322, 1672
MalI GATC 5 cut(s) 102, 694, 978, 1316, 1668
MboI GATC 5 cut(s) 100, 692, 976, 1314, 1666
MfeI CAATTG 1 cut(s) 993
MflI RGATCY 3 cut(s) 976, 1314, 1666
MhlI GDGCHC 2 cut(s) 361, 587
MluCI AATT 5 cut(s) 306, 440, 993, 1043, 1475
MluI ACGCGT 1 cut(s) 1579
MlyI GAGTC 3 cut(s) 468, 1295, 1356
MmeI TCCRAC 4 cut(s) 187, 769, 777, 1255
Mph1103I ATGCAT 1 cut(s) 1501
MroXI GAANNNNTTC 1 cut(s) 453
MseI TTAA 1 cut(s) 321
MslI CAYNNNNRTG 3 cut(s) 354, 462, 1580
MspI CCGG 2 cut(s) 206, 1031
MspR9I CCNGG 1 cut(s) 680
MunI CAATTG 1 cut(s) 993
Mva1269I GAATGC 1 cut(s) 1433
MvaI CCWGG 1 cut(s) 680
MvnI CGCG 1 cut(s) 1581
MwoI GCNNNNNNNGC 4 cut(s) 148, 382, 1136, 1166
NcoI CCATGG 1 cut(s) 1017
NdeII GATC 5 cut(s) 100, 692, 976, 1314, 1666
NheI GCTAGC 2 cut(s) 806, 1255
NlaIV GGNNCC 4 cut(s) 201, 328, 1184, 1630
NmuCI GTSAC 3 cut(s) 1234, 1322, 1672
NsiI ATGCAT 1 cut(s) 1501
NspI RCATGY 5 cut(s) 142, 614, 670, 1412, 1549
NspV TTCGAA 3 cut(s) 276, 596, 1480
OliI CACNNNNGTG 2 cut(s) 354, 1580
PaeI GCATGC 1 cut(s) 614
PciI ACATGT 2 cut(s) 666, 1408
PciSI GCTCTTC 1 cut(s) 366
PctI GAATGC 1 cut(s) 1433
PdmI GAANNNNTTC 1 cut(s) 453
PfeI GAWTC 3 cut(s) 449, 619, 1570
PflMI CCANNNNNTGG 1 cut(s) 93
PkrI GCNGC 3 cut(s) 303, 384, 571
PleI GAGTC 3 cut(s) 468, 1295, 1356
PpsI GAGTC 3 cut(s) 468, 1295, 1356
Ppu21I YACGTR 1 cut(s) 698
PscI ACATGT 2 cut(s) 666, 1408
PsiI TTATAA 2 cut(s) 117, 393
Psp6I CCWGG 1 cut(s) 678
PspGI CCWGG 1 cut(s) 678
PspN4I GGNNCC 4 cut(s) 201, 328, 1184, 1630
PstNI CAGNNNCTG 1 cut(s) 685
PsuI RGATCY 3 cut(s) 976, 1314, 1666
RsaI GTAC 4 cut(s) 488, 1026, 1593, 1598
RsaNI GTAC 4 cut(s) 487, 1025, 1592, 1597
RseI CAYNNNNRTG 3 cut(s) 354, 462, 1580
SapI GCTCTTC 1 cut(s) 366
SaqAI TTAA 1 cut(s) 321
SatI GCNGC 3 cut(s) 302, 383, 570
Sau3AI GATC 5 cut(s) 100, 692, 976, 1314, 1666
SchI GAGTC 3 cut(s) 468, 1295, 1356
ScrFI CCNGG 1 cut(s) 680
SduI GDGCHC 2 cut(s) 361, 587
SfaNI GCATC 5 cut(s) 180, 763, 1486, 1548, 1661
SfcI CTRYAG 1 cut(s) 560
SfuI TTCGAA 3 cut(s) 276, 596, 1480
SmiMI CAYNNNNRTG 3 cut(s) 354, 462, 1580
SphI GCATGC 1 cut(s) 614
Sse9I AATT 5 cut(s) 306, 440, 993, 1043, 1475
SsiI CCGC 2 cut(s) 203, 590
SspMI CTAG 8 cut(s) 527, 539, 650, 807, 894, 974, 987, 1256
StyD4I CCNGG 1 cut(s) 678
StyI CCWWGG 1 cut(s) 1017
TaaI ACNGT 5 cut(s) 564, 1118, 1448, 1596, 1678
TaiI ACGT 2 cut(s) 70, 700
TaqI TCGA 8 cut(s) 64, 276, 444, 596, 1122, 1308, 1473, 1480
TasI AATT 5 cut(s) 306, 440, 993, 1043, 1475
TatI WGTACW 3 cut(s) 1024, 1591, 1596
TfiI GAWTC 3 cut(s) 449, 619, 1570
Tru1I TTAA 1 cut(s) 321
Tru9I TTAA 1 cut(s) 321
TscAI CASTG 1 cut(s) 1327
TseFI GTSAC 3 cut(s) 1234, 1322, 1672
TseI GCWGC 3 cut(s) 301, 382, 569
Tsp45I GTSAC 3 cut(s) 1234, 1322, 1672
TspDTI ATGAA 3 cut(s) 1055, 1157, 1530
TspGWI ACGGA 1 cut(s) 4
TspRI CASTG 1 cut(s) 1327
Van91I CCANNNNNTGG 1 cut(s) 93
XapI RAATTY 4 cut(s) 306, 440, 1043, 1475
XbaI TCTAGA 1 cut(s) 526
XceI RCATGY 5 cut(s) 142, 614, 670, 1412, 1549
XcmI CCANNNNNNNNNTGG 1 cut(s) 686
XmiI GTMKAC 1 cut(s) 867
XmnI GAANNNNTTC 1 cut(s) 453
XspI CTAG 8 cut(s) 527, 539, 650, 807, 894, 974, 987, 1256
Zsp2I ATGCAT 1 cut(s) 1501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.