MD16G1070800.v1.1

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000053
Physical Location & Seq
Forward (+)
4972251 .. 4974896
2646 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1070800.v1.1.491

Sequence Viewer

Length: 1722 bp
ATGGTTCCTCGTACTTGCATAGCAATCTTAAAGCTACAGAAAGAAAGCATGGAAAGCACAATGGTTCAGGGTTCAGCAGCTTCATCACAAAATGAAAGTGAAATTGTTCGGCCTTCAAAATATTATCACCCAAGCATTTGGAGAGATCAATTCATCAATTATGACGACTCTCAAGAAATTAGGACTTGTGCCGACATTCAACACCAAGTTAACCACCTCAAAGAAGCAGTGAGGAGAAAAATATTCACAGCAATTGCAAGTGACTTTTCCCATCAACTAAAGTTAATTGATATTATTCAGCGCCTTGGCGTGGCATACCATTTTGAGAGAGAAATCGAAGAAGCACTGGATAATATGCATGCTACAATATTTGACTACCATGGCGACAATTATGGCGATCTATATAGTGTTGCTCTTGGTTTTCGGATACTAAGACAACATGGATATAACGCTACATGTGCTATGTTCAACAGGTTCAAAGATGATGAGAATGGTAGTTTCAAGGAAAGCTTGACTGATGACCTGTCGGGCATGCTAAGCTTATATGAAGCATCCCACCTGAGGGTGCATGGAGAAGATATACTAGAGGAGGCTTTTAATTTCACCACCACTCACCTCGAGTCGGCAGCAACCAAAGCAAGCTATCCGCTATCAGCACAAATAACTCAAGCTTTAGAGAGACCTCTGCGAAGAAGTCTAGAGAGATTATCTGCCAGGCATTACACGTCAATCTACCAAGATGAAGCTTCCCATGACGAAGCTTTACTGAAACTTGCAAAGTTAGATTTCAATCTTGTTCAGGCTTTACACAAAAAGGAACTCCAAGAGATTACAAGGTGGTGGAGAGCACTAGAGTTTGACAGGAAGCTGCCATTTGCAAGAGATAGGGTGGTGGAGTTGTACTTTTGGACAGTGGGAGTATATTTTGAACCCCAGTACTCTGTTGGGAGAAAAATTCTGGCAAAAGTGAACGTCTTATTAACTGTGATGGATGATATTTACGATTCATTTGGTACATTTGAAGAACTCGTGACCTTTACTGAGGCAATCGACAGGTGGGATGTCAATGGCCTTGATGAACTGCAAGACTATATGCAAATATTTTACTATGCACTTCTGAATCTTTTTAATGAAATTGAGGAAGAGATGATAAAGAAAGGAAATTCATACCGGGTTCCCTATGCAATAGAAGCTATGAAAGGTCAAGCTCATTCCTATTTGGACGAGGCCCGATGGTTACACGAAGGACGAATTCCAAGCATGGAGGAGTATATGCGTGTTGCAACAGTTTCTATTAGCTACACCTTCAATACAGCCATCACTTTACTTGGCATGGGAGATATTGTAACAAAGCAGTCATTTGACTGGTTGTTTAATGACCCCAAAATTATTAGGGCTGCAAATACCATCTTCAGAATCATGGATGACATTGTTTCGACCGACGCTGATGCTATTACTTGTGTTCATTGCTACATGAAGCAATACGGGGTCTCAAAGCAAGAGGCAGTTGATGTTTTTGAGAAACAAATTATGGATTTGTGGAAGGATATAAACGAGGAGTTCCTTAGATCAACAGTTGTTCCAATGCCCGTCCTCACGCGCGTCCTCAATCTAGTACGAGTACCTGATCTCCTTTACAAAGGGGACGATGGATTCACACGAGTGGGAAAGGTGACAAAAGATGGTGTCGCTTCAATCTGTATCGATCCAGTCCCTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

574

Amino Acids

66.27

Weight (kDa)

5.2

Isoelectric Point (pI)

42.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 46 - 225 9.8e-56 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 284 - 516 1.4e-88 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 322 - 516 9.1e-51 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 1600, 1602
AciI CCGC 1 cut(s) 647
AclWI GGATC 1 cut(s) 1700
AcsI RAATTY 3 cut(s) 954, 1162, 1251
AcuI CTGAAG 1 cut(s) 1396
AfaI GTAC 6 cut(s) 13, 902, 938, 1015, 1617, 1623
AfiI CCNNNNNNNGG 4 cut(s) 310, 561, 562, 622
AflIII ACRYGT 2 cut(s) 455, 723
AjiI CACGTC 1 cut(s) 726
AjnI CCWGG 1 cut(s) 713
AleI CACNNNNGTG 1 cut(s) 1661
Alw21I GWGCWC 1 cut(s) 850
Alw26I GTCTC 2 cut(s) 673, 1495
AlwI GGATC 1 cut(s) 1700
Ama87I CYCGRG 1 cut(s) 617
AoxI GGCC 3 cut(s) 110, 1069, 1227
ApeKI GCWGC 4 cut(s) 77, 626, 868, 1397
ApoI RAATTY 3 cut(s) 954, 1162, 1251
Asp700I GAANNNNTTC 1 cut(s) 105
AspLEI GCGC 2 cut(s) 303, 1602
AspS9I GGNCC 1 cut(s) 1228
AsuC2I CCSGG 1 cut(s) 1172
AsuHPI GGTGA 4 cut(s) 119, 595, 605, 1684
AvaI CYCGRG 1 cut(s) 617
AxyI CCTNAGG 1 cut(s) 560
BauI CACGAG 2 cut(s) 1028, 1659
Bbv12I GWGCWC 1 cut(s) 850
BbvI GCAGC 4 cut(s) 89, 638, 855, 1384
BccI CCATC 7 cut(s) 279, 982, 1227, 1325, 1415, 1643, 1676
BcgI CGANNNNNNTGC 2 cut(s) 1430, 1464
BciT130I CCWGG 1 cut(s) 715
BciVI GTATCC 1 cut(s) 420
BcnI CCSGG 1 cut(s) 1172
BcoDI GTCTC 2 cut(s) 673, 1495
BfaI CTAG 4 cut(s) 584, 698, 851, 1613
BfmI CTRYAG 1 cut(s) 35
BfoI RGCGCY 1 cut(s) 304
BfuI GTATCC 1 cut(s) 420
BisI GCNGC 4 cut(s) 78, 627, 869, 1398
BlpI GCTNAGC 1 cut(s) 536
BlsI GCNGC 4 cut(s) 79, 628, 870, 1399
BmcAI AGTACT 1 cut(s) 938
Bme1390I CCNGG 2 cut(s) 715, 1172
BmeT110I CYCGRG 1 cut(s) 617
BmgBI CACGTC 1 cut(s) 726
BmgT120I GGNCC 1 cut(s) 1228
BmiI GGNNCC 2 cut(s) 6, 1176
BmrFI CCNGG 2 cut(s) 715, 1172
BmrI ACTGGG 1 cut(s) 928
BmsI GCATC 2 cut(s) 560, 1438
BmuI ACTGGG 1 cut(s) 928
Bpu1102I GCTNAGC 1 cut(s) 536
BpuEI CTTGAG 2 cut(s) 156, 651
BpuMI CCSGG 1 cut(s) 1172
Bsa29I ATCGAT 1 cut(s) 1704
BsaBI GATNNNNATC 1 cut(s) 789
BsaI GGTCTC 2 cut(s) 673, 1495
BsaJI CCNNGG 2 cut(s) 304, 379
BsaXI ACNNNNNCTCC 4 cut(s) 1329, 1359, 1614, 1644
Bsc4I CCNNNNNNNGG 4 cut(s) 310, 561, 562, 622
Bse1I ACTGG 4 cut(s) 351, 934, 1370, 1709
Bse21I CCTNAGG 1 cut(s) 560
Bse3DI GCAATG 1 cut(s) 1465
Bse8I GATNNNNATC 1 cut(s) 789
BseBI CCWGG 1 cut(s) 715
BseCI ATCGAT 1 cut(s) 1704
BseDI CCNNGG 2 cut(s) 304, 379
BseGI GGATG 4 cut(s) 551, 997, 1066, 1429
BseJI GATNNNNATC 1 cut(s) 789
BseLI CCNNNNNNNGG 4 cut(s) 310, 561, 562, 622
BseMI GCAATG 1 cut(s) 1465
BseMII CTCAG 2 cut(s) 551, 1032
BseNI ACTGG 4 cut(s) 351, 934, 1370, 1709
BseRI GAGGAG 4 cut(s) 247, 602, 1280, 1571
BseXI GCAGC 4 cut(s) 89, 638, 855, 1384
Bsh1236I CGCG 2 cut(s) 1600, 1602
Bsh1285I CGRYCG 1 cut(s) 1440
BshFI GGCC 3 cut(s) 112, 1071, 1229
BshVI ATCGAT 1 cut(s) 1704
BsiEI CGRYCG 1 cut(s) 1440
BsiHKAI GWGCWC 1 cut(s) 850
BsiHKCI CYCGRG 1 cut(s) 617
BsiSI CCGG 1 cut(s) 1171
BslFI GGGAC 2 cut(s) 1658, 1697
BslI CCNNNNNNNGG 4 cut(s) 310, 561, 562, 622
BsmAI GTCTC 2 cut(s) 673, 1495
BsmFI GGGAC 2 cut(s) 1658, 1697
BsnI GGCC 3 cut(s) 112, 1071, 1229
Bso31I GGTCTC 2 cut(s) 673, 1495
BsoBI CYCGRG 1 cut(s) 617
Bsp1286I GDGCHC 1 cut(s) 850
Bsp143I GATC 5 cut(s) 145, 397, 1568, 1627, 1705
Bsp1720I GCTNAGC 1 cut(s) 536
Bsp19I CCATGG 1 cut(s) 379
BspACI CCGC 1 cut(s) 647
BspANI GGCC 3 cut(s) 112, 1071, 1229
BspCNI CTCAG 2 cut(s) 552, 1033
BspDI ATCGAT 1 cut(s) 1704
BspFNI CGCG 2 cut(s) 1600, 1602
BspLI GGNNCC 2 cut(s) 6, 1176
BspPI GGATC 1 cut(s) 1700
BspTNI GGTCTC 2 cut(s) 673, 1495
BsrDI GCAATG 1 cut(s) 1465
BsrI ACTGG 4 cut(s) 351, 934, 1370, 1709
BssECI CCNNGG 2 cut(s) 304, 379
BssMI GATC 5 cut(s) 145, 397, 1568, 1627, 1705
BssSI CACGAG 2 cut(s) 1028, 1659
BssT1I CCWWGG 2 cut(s) 304, 379
Bst2BI CACGAG 2 cut(s) 1028, 1659
Bst2UI CCWGG 1 cut(s) 715
Bst4CI ACNGT 4 cut(s) 913, 985, 1288, 1576
Bst6I CTCTTC 1 cut(s) 1137
BstC8I GCNNGC 3 cut(s) 360, 533, 640
BstDEI CTNAG 5 cut(s) 431, 536, 560, 1041, 1565
BstDSI CCRYGG 1 cut(s) 379
BstF5I GGATG 4 cut(s) 551, 997, 1066, 1429
BstFNI CGCG 2 cut(s) 1600, 1602
BstH2I RGCGCY 1 cut(s) 304
BstHHI GCGC 2 cut(s) 303, 1602
BstKTI GATC 5 cut(s) 148, 400, 1571, 1630, 1708
BstMAI GTCTC 2 cut(s) 673, 1495
BstMBI GATC 5 cut(s) 145, 397, 1568, 1627, 1705
BstMCI CGRYCG 1 cut(s) 1440
BstMWI GCNNNNNNNGC 5 cut(s) 54, 458, 537, 635, 1190
BstNI CCWGG 1 cut(s) 715
BstNSI RCATGY 3 cut(s) 362, 459, 535
BstSCI CCNGG 2 cut(s) 713, 1170
BstSFI CTRYAG 1 cut(s) 35
BstUI CGCG 2 cut(s) 1600, 1602
BstV1I GCAGC 4 cut(s) 89, 638, 855, 1384
BstXI CCANNNNNNTGG 1 cut(s) 138
Bsu15I ATCGAT 1 cut(s) 1704
Bsu36I CCTNAGG 1 cut(s) 560
BsuI GTATCC 1 cut(s) 420
BsuRI GGCC 3 cut(s) 112, 1071, 1229
BsuTUI ATCGAT 1 cut(s) 1704
BtgI CCRYGG 1 cut(s) 379
BtrI CACGTC 1 cut(s) 726
BtsCI GGATG 4 cut(s) 551, 997, 1066, 1429
BtsI GCAGTG 1 cut(s) 234
BtsIMutI CAGTG 3 cut(s) 234, 344, 918
Cac8I GCNNGC 3 cut(s) 360, 533, 640
CfoI GCGC 2 cut(s) 303, 1602
Cfr13I GGNCC 1 cut(s) 1228
ClaI ATCGAT 1 cut(s) 1704
CseI GACGC 2 cut(s) 1451, 1591
Csp6I GTAC 6 cut(s) 12, 901, 937, 1014, 1616, 1622
CviQI GTAC 6 cut(s) 12, 901, 937, 1014, 1616, 1622
DdeI CTNAG 5 cut(s) 431, 536, 560, 1041, 1565
DpnI GATC 5 cut(s) 147, 399, 1570, 1629, 1707
DpnII GATC 5 cut(s) 145, 397, 1568, 1627, 1705
Eam1104I CTCTTC 1 cut(s) 1137
EarI CTCTTC 1 cut(s) 1137
Eco130I CCWWGG 2 cut(s) 304, 379
Eco31I GGTCTC 2 cut(s) 673, 1495
Eco57I CTGAAG 1 cut(s) 1396
Eco81I CCTNAGG 1 cut(s) 560
Eco88I CYCGRG 1 cut(s) 617
EcoRI GAATTC 1 cut(s) 1251
EcoRII CCWGG 1 cut(s) 713
EcoT14I CCWWGG 2 cut(s) 304, 379
EcoT22I ATGCAT 1 cut(s) 360
ErhI CCWWGG 2 cut(s) 304, 379
FalI AAGNNNNNCTT 2 cut(s) 494, 526
FaqI GGGAC 2 cut(s) 1658, 1697
Fnu4HI GCNGC 4 cut(s) 78, 627, 869, 1398
FokI GGATG 4 cut(s) 538, 1004, 1073, 1436
Fsp4HI GCNGC 4 cut(s) 78, 627, 869, 1398
FspBI CTAG 4 cut(s) 584, 698, 851, 1613
GlaI GCGC 2 cut(s) 302, 1601
GluI GCNGC 4 cut(s) 78, 627, 869, 1398
HaeII RGCGCY 1 cut(s) 304
HaeIII GGCC 3 cut(s) 112, 1071, 1229
HapII CCGG 1 cut(s) 1171
HgaI GACGC 2 cut(s) 1451, 1591
HhaI GCGC 2 cut(s) 303, 1602
Hin6I GCGC 2 cut(s) 301, 1600
HinP1I GCGC 2 cut(s) 301, 1600
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HindIII AAGCTT 5 cut(s) 508, 538, 669, 744, 759
HinfI GANTC 6 cut(s) 167, 620, 1004, 1120, 1416, 1653
HpaI GTTAAC 1 cut(s) 211
HpaII CCGG 1 cut(s) 1171
HphI GGTGA 4 cut(s) 119, 595, 605, 1684
Hpy166II GTNNAC 2 cut(s) 211, 970
Hpy188I TCNGA 3 cut(s) 426, 1119, 1415
Hpy188III TCNNGA 3 cut(s) 173, 698, 1030
Hpy8I GTNNAC 2 cut(s) 211, 970
Hpy99I CGWCG 1 cut(s) 1445
HpyAV CCTTC 4 cut(s) 123, 1238, 1315, 1537
HpyCH4III ACNGT 4 cut(s) 913, 985, 1288, 1576
HpyCH4IV ACGT 2 cut(s) 725, 972
HpyF10VI GCNNNNNNNGC 5 cut(s) 54, 458, 537, 635, 1190
HpyF3I CTNAG 5 cut(s) 431, 536, 560, 1041, 1565
HpySE526I ACGT 2 cut(s) 725, 972
HspAI GCGC 2 cut(s) 301, 1600
KspAI GTTAAC 1 cut(s) 211
Kzo9I GATC 5 cut(s) 145, 397, 1568, 1627, 1705
Lsp1109I GCAGC 4 cut(s) 89, 638, 855, 1384
LweI GCATC 2 cut(s) 560, 1438
MaeI CTAG 4 cut(s) 584, 698, 851, 1613
MaeII ACGT 2 cut(s) 725, 972
MaeIII GTNAC 5 cut(s) 260, 1030, 1236, 1345, 1672
MalI GATC 5 cut(s) 147, 399, 1570, 1629, 1707
MboI GATC 5 cut(s) 145, 397, 1568, 1627, 1705
MboII GAAGA 6 cut(s) 350, 587, 702, 1034, 1154, 1402
MfeI CAATTG 1 cut(s) 252
MhlI GDGCHC 1 cut(s) 850
MlyI GAGTC 2 cut(s) 161, 629
Mph1103I ATGCAT 1 cut(s) 360
MroXI GAANNNNTTC 1 cut(s) 105
MseI TTAA 7 cut(s) 29, 210, 284, 597, 980, 1128, 1374
MslI CAYNNNNRTG 1 cut(s) 1661
MspI CCGG 1 cut(s) 1171
MspR9I CCNGG 2 cut(s) 715, 1172
MunI CAATTG 1 cut(s) 252
MvaI CCWGG 1 cut(s) 715
MvnI CGCG 2 cut(s) 1600, 1602
MwoI GCNNNNNNNGC 5 cut(s) 54, 458, 537, 635, 1190
NciI CCSGG 1 cut(s) 1172
NcoI CCATGG 1 cut(s) 379
NdeII GATC 5 cut(s) 145, 397, 1568, 1627, 1705
NlaIV GGNNCC 2 cut(s) 6, 1176
NmuCI GTSAC 3 cut(s) 260, 1030, 1672
NsiI ATGCAT 1 cut(s) 360
NspI RCATGY 3 cut(s) 362, 459, 535
OliI CACNNNNGTG 1 cut(s) 1661
PaeI GCATGC 2 cut(s) 362, 535
PaeR7I CTCGAG 1 cut(s) 617
PciI ACATGT 1 cut(s) 455
PdmI GAANNNNTTC 1 cut(s) 105
PfeI GAWTC 4 cut(s) 1004, 1120, 1416, 1653
PkrI GCNGC 4 cut(s) 79, 628, 870, 1399
PleI GAGTC 2 cut(s) 161, 628
PpsI GAGTC 2 cut(s) 161, 628
PscI ACATGT 1 cut(s) 455
Psp6I CCWGG 1 cut(s) 713
PspGI CCWGG 1 cut(s) 713
PspN4I GGNNCC 2 cut(s) 6, 1176
PspPI GGNCC 1 cut(s) 1228
PspXI VCTCGAGB 1 cut(s) 617
RsaI GTAC 6 cut(s) 13, 902, 938, 1015, 1617, 1623
RsaNI GTAC 6 cut(s) 12, 901, 937, 1014, 1616, 1622
RseI CAYNNNNRTG 1 cut(s) 1661
SaqAI TTAA 7 cut(s) 29, 210, 284, 597, 980, 1128, 1374
SatI GCNGC 4 cut(s) 78, 627, 869, 1398
Sau3AI GATC 5 cut(s) 145, 397, 1568, 1627, 1705
Sau96I GGNCC 1 cut(s) 1228
ScaI AGTACT 1 cut(s) 938
SchI GAGTC 2 cut(s) 161, 629
ScrFI CCNGG 2 cut(s) 715, 1172
SduI GDGCHC 1 cut(s) 850
SfaNI GCATC 2 cut(s) 560, 1438
SfcI CTRYAG 1 cut(s) 35
Sfr274I CTCGAG 1 cut(s) 617
SlaI CTCGAG 1 cut(s) 617
SmiMI CAYNNNNRTG 1 cut(s) 1661
SmlI CTYRAG 3 cut(s) 171, 617, 666
SmoI CTYRAG 3 cut(s) 171, 617, 666
SphI GCATGC 2 cut(s) 362, 535
SsiI CCGC 1 cut(s) 647
SspI AATATT 4 cut(s) 122, 243, 369, 1101
SspMI CTAG 4 cut(s) 584, 698, 851, 1613
StyD4I CCNGG 2 cut(s) 713, 1170
StyI CCWWGG 2 cut(s) 304, 379
TaaI ACNGT 4 cut(s) 913, 985, 1288, 1576
TaiI ACGT 2 cut(s) 728, 975
TaqI TCGA 5 cut(s) 336, 618, 1050, 1436, 1704
TaqII GACCGA 1 cut(s) 1454
TatI WGTACW 2 cut(s) 900, 936
TfiI GAWTC 4 cut(s) 1004, 1120, 1416, 1653
Tru1I TTAA 7 cut(s) 29, 210, 284, 597, 980, 1128, 1374
Tru9I TTAA 7 cut(s) 29, 210, 284, 597, 980, 1128, 1374
TscAI CASTG 3 cut(s) 234, 351, 918
TseFI GTSAC 3 cut(s) 260, 1030, 1672
TseI GCWGC 4 cut(s) 77, 626, 868, 1397
Tsp45I GTSAC 3 cut(s) 260, 1030, 1672
TspRI CASTG 3 cut(s) 234, 351, 918
XapI RAATTY 3 cut(s) 954, 1162, 1251
XbaI TCTAGA 1 cut(s) 697
XceI RCATGY 3 cut(s) 362, 459, 535
XcmI CCANNNNNNNNNTGG 1 cut(s) 941
XhoI CTCGAG 1 cut(s) 617
XmnI GAANNNNTTC 1 cut(s) 105
XspI CTAG 4 cut(s) 584, 698, 851, 1613
ZrmI AGTACT 1 cut(s) 938
Zsp2I ATGCAT 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.