RchiOBHm_Chr6g0305391

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000053
Physical Location & Seq
Reverse (-)
64418757 .. 64421276
2520 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27445

Sequence Viewer

Length: 1713 bp
ATGTACGGCAAATCAACCATGCCTGTCCAAGCTACTCCAGCAGAAGCTGAATCCCATATCAATAATGCTAAGCCAGAAGTTGTTCGGCGCACAGCAAATTTTCAATCTAGCGTATGGGGAGATCAGTTTGCTAATTATGCCCATGACATTATAACTCAAGCTCATATGCAACAACAAGTTGAGGAGCTGAAACTAGTAGTGAGGAAGGAAGTATTCACTAATGCTGCTGATGATGATTTTTCACATCAACTGAAGCTAATTGATGCAATCCAGCGCCTCGGTGTGGCTTACCATTTTGAAACTGAAATAGAACAAGCCCTGGAACGTATACATGCTACCTATCAAGATATTCATGATGATGATGGTGGTGATCTATACAATGTTGCTCTTCGTTTTCGACTTCTCAGACAACATGGATATAGTGTTTCCTGCGATGTATTCAACAAGTTCAAAGATGCTAACGGTGACTTCAAGGAAAGCTTAGTTACTGATGTTTCAGGTATGCTAAGCTTCTATGAGGCATCCCATCAAATGATGCATGGAGAAAAATTACTTGAAGAGGCTCTCGATTTTACCACCACTCATCTCAAGACTGCAACAACTTTAACCAGTGCAAGCTCTTTACTAAAGGCACAAATAACTGAAGCCCTAGAGAGACCTCTACTAAAAACTATAGAGAGGTTAGGTGCTCGGCGTTACATGTCAATATACCAAGATGAAGCTTCACACAACGAGGAGTTACTGAAACTTGCAAAGTTAGATTTTAATTTTGTACAGTGTTTACACAAAAAGGAACTCAGTGACATTATGAGATGGTATAAGGAACTAGACTTTGCAAGGAGGATGCCTTTTGCTCGAGATAGGATCGTGGAGTTGTTCTTTTGGGAAGTGGGAATATATTTTGAACCCCAATACGTGTTCGGGAGAAACATTCTCACCAAACTGGGTGAGATAGTAACAGTAATGGATGATGTGTATGATGCATTTGGTACATTTGAAGAACTAGTCAGATTAACTGAAGCAATCGAGAGGTGGGATGCTAGTTGCATGGATCAACTGCCAGACTATATGCAACCACTTTATCAAACACTTCTGGATGTTTTCAATGAAGTTGAGGAGGAATTGATACAGCAAGGAAGATCATACCGACTTTACTATGCAAAAGAAATTATGAAGAATCAAGCCCGGCTCTACTTCGCTGAGGCCAGATGGTTCCACGAAGGATGCACCCCAAGAATGGATGAGTATATGCGTGTTGCGGCAGATTCTGTCGGTAACACGATGATTGCAGTCGTATCTTTAGTAGGCATGGGAGACATTATAACAAAAGAGGCATTTGAGTGGTTGACCAATAATCCTAAAATCCTTAGAGCTTCGAATACCATATTTAGGCTTATGGATGATATTGCAGGGCACAAGTTTGAGAAAGAGAGAGGGCATGTTGCTTCTAGTATTGATTGCTACATGAATGAATATGGGGTCTCAGAGCAAGAGACGATTGATGTCTTTAACAAACGAATTGCGGATTCGTGGAAGGATATAAATGAAGAGTTTATGAGACCCACTGCAGTGCCAATGGCTGTGCTTAATCTTGTTCTTAACCTCACTCAAGTGGTTGATCTCCTTTACAAAAGGGGTGATGCCTTCACGCATGTTGGAAAACTGATGAAAGATTGTATTGCTGCACTGTTTATTGATCCAGTGCCCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

570

Amino Acids

65.88

Weight (kDa)

5.07

Isoelectric Point (pI)

42.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 38 - 217 6.3e-58 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 274 - 513 2.1e-95 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 315 - 513 3.1e-59 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 152, 1322
AccI GTMKAC 1 cut(s) 328
AciI CCGC 2 cut(s) 1259, 1523
AclWI GGATC 3 cut(s) 872, 1059, 1691
AcsI RAATTY 1 cut(s) 97
AcuI CTGAAG 3 cut(s) 272, 663, 1038
AfaI GTAC 3 cut(s) 5, 774, 991
AfiI CCNNNNNNNGG 3 cut(s) 283, 1237, 1389
AflIII ACRYGT 2 cut(s) 699, 915
AgsI TTSAA 9 cut(s) 104, 299, 442, 451, 472, 557, 905, 998, 1105
AhlI ACTAGT 2 cut(s) 193, 1003
AjnI CCWGG 1 cut(s) 318
AleI CACNNNNGTG 2 cut(s) 1568, 1610
Alw21I GWGCWC 1 cut(s) 691
Alw26I GTCTC 5 cut(s) 649, 1308, 1486, 1487, 1552
AlwI GGATC 3 cut(s) 872, 1059, 1691
AlwNI CAGNNNCTG 2 cut(s) 47, 1268
Ama87I CYCGRG 1 cut(s) 855
AoxI GGCC 1 cut(s) 1203
ApeKI GCWGC 2 cut(s) 224, 1682
ApoI RAATTY 1 cut(s) 97
Asp700I GAANNNNTTC 1 cut(s) 81
AspLEI GCGC 2 cut(s) 90, 276
AsuC2I CCSGG 1 cut(s) 1186
AsuHPI GGTGA 5 cut(s) 380, 476, 928, 959, 1649
AsuII TTCGAA 1 cut(s) 1376
AvaI CYCGRG 1 cut(s) 855
BaeGI GKGCMC 2 cut(s) 1416, 1707
Bbv12I GWGCWC 1 cut(s) 691
BbvCI CCTCAGC 1 cut(s) 1200
BbvI GCAGC 2 cut(s) 211, 1669
BccI CCATC 4 cut(s) 356, 534, 807, 1203
BceAI ACGGC 1 cut(s) 22
BciT130I CCWGG 1 cut(s) 320
BcnI CCSGG 1 cut(s) 1186
BcoDI GTCTC 5 cut(s) 649, 1308, 1486, 1487, 1552
BcuI ACTAGT 2 cut(s) 193, 1003
BfaI CTAG 7 cut(s) 108, 194, 650, 827, 1004, 1041, 1449
BfmI CTRYAG 2 cut(s) 672, 1566
BfoI RGCGCY 1 cut(s) 277
BisI GCNGC 3 cut(s) 225, 1260, 1683
BlpI GCTNAGC 2 cut(s) 69, 506
BlsI GCNGC 3 cut(s) 226, 1261, 1684
Bme1390I CCNGG 2 cut(s) 320, 1186
BmeT110I CYCGRG 1 cut(s) 855
BmiI GGNNCC 1 cut(s) 1214
BmrFI CCNGG 2 cut(s) 320, 1186
BmrI ACTGGG 1 cut(s) 953
BmsI GCATC 9 cut(s) 253, 445, 525, 530, 834, 970, 1027, 1214, 1630
BmuI ACTGGG 1 cut(s) 953
BpmI CTGGAG 1 cut(s) 21
Bpu10I CCTNAGC 1 cut(s) 1200
Bpu1102I GCTNAGC 2 cut(s) 69, 506
Bpu14I TTCGAA 1 cut(s) 1376
BpuEI CTTGAG 3 cut(s) 141, 572, 1593
BpuMI CCSGG 1 cut(s) 1186
BsaAI YACGTR 1 cut(s) 916
BsaI GGTCTC 3 cut(s) 649, 1486, 1552
BsaJI CCNNGG 2 cut(s) 277, 318
Bsc4I CCNNNNNNNGG 3 cut(s) 283, 1237, 1389
Bse1I ACTGG 3 cut(s) 609, 948, 1700
BseBI CCWGG 1 cut(s) 320
BseDI CCNNGG 2 cut(s) 277, 318
BseGI GGATG 8 cut(s) 521, 849, 973, 1042, 1102, 1229, 1246, 1405
BseLI CCNNNNNNNGG 3 cut(s) 283, 1237, 1389
BseMII CTCAG 4 cut(s) 418, 811, 1191, 1497
BseNI ACTGG 3 cut(s) 609, 948, 1700
BseRI GAGGAG 3 cut(s) 197, 749, 1130
BseSI GKGCMC 2 cut(s) 1416, 1707
BseXI GCAGC 2 cut(s) 211, 1669
BsgI GTGCAG 1 cut(s) 1668
BshFI GGCC 1 cut(s) 1205
BsiHKAI GWGCWC 1 cut(s) 691
BsiHKCI CYCGRG 1 cut(s) 855
BsiSI CCGG 1 cut(s) 1186
BslI CCNNNNNNNGG 3 cut(s) 283, 1237, 1389
BsmAI GTCTC 5 cut(s) 649, 1308, 1486, 1487, 1552
BsmBI CGTCTC 1 cut(s) 1487
BsnI GGCC 1 cut(s) 1205
Bso31I GGTCTC 3 cut(s) 649, 1486, 1552
BsoBI CYCGRG 1 cut(s) 855
Bsp119I TTCGAA 1 cut(s) 1376
Bsp1286I GDGCHC 3 cut(s) 691, 1416, 1707
Bsp1407I TGTACA 1 cut(s) 772
Bsp143I GATC 7 cut(s) 121, 370, 864, 1051, 1139, 1618, 1696
Bsp1720I GCTNAGC 2 cut(s) 69, 506
BspACI CCGC 2 cut(s) 1259, 1523
BspANI GGCC 1 cut(s) 1205
BspCNI CTCAG 4 cut(s) 417, 810, 1192, 1496
BspHI TCATGA 1 cut(s) 352
BspLI GGNNCC 1 cut(s) 1214
BspMAI CTGCAG 1 cut(s) 1570
BspPI GGATC 3 cut(s) 872, 1059, 1691
BspQI GCTCTTC 1 cut(s) 393
BspT104I TTCGAA 1 cut(s) 1376
BspTNI GGTCTC 3 cut(s) 649, 1486, 1552
BsrGI TGTACA 1 cut(s) 772
BsrI ACTGG 3 cut(s) 609, 948, 1700
BssECI CCNNGG 2 cut(s) 277, 318
BssMI GATC 7 cut(s) 121, 370, 864, 1051, 1139, 1618, 1696
BssNAI GTATAC 1 cut(s) 329
Bst1107I GTATAC 1 cut(s) 329
Bst2UI CCWGG 1 cut(s) 320
Bst4CI ACNGT 4 cut(s) 464, 777, 961, 1689
Bst6I CTCTTC 3 cut(s) 393, 552, 1542
BstAUI TGTACA 1 cut(s) 772
BstBAI YACGTR 1 cut(s) 916
BstBI TTCGAA 1 cut(s) 1376
BstC8I GCNNGC 1 cut(s) 616
BstDEI CTNAG 8 cut(s) 69, 404, 481, 506, 797, 1200, 1367, 1483
BstF5I GGATG 8 cut(s) 521, 849, 973, 1042, 1102, 1229, 1246, 1405
BstH2I RGCGCY 1 cut(s) 277
BstHHI GCGC 2 cut(s) 90, 276
BstKTI GATC 7 cut(s) 124, 373, 867, 1054, 1142, 1621, 1699
BstMAI GTCTC 5 cut(s) 649, 1308, 1486, 1487, 1552
BstMBI GATC 7 cut(s) 121, 370, 864, 1051, 1139, 1618, 1696
BstMWI GCNNNNNNNGC 2 cut(s) 38, 137
BstNI CCWGG 1 cut(s) 320
BstNSI RCATGY 4 cut(s) 335, 703, 1442, 1655
BstSCI CCNGG 2 cut(s) 318, 1184
BstSFI CTRYAG 2 cut(s) 672, 1566
BstSLI GKGCMC 2 cut(s) 1416, 1707
BstV1I GCAGC 2 cut(s) 211, 1669
BstZ17I GTATAC 1 cut(s) 329
BsuRI GGCC 1 cut(s) 1205
BtgZI GCGATG 1 cut(s) 447
BtsCI GGATG 8 cut(s) 521, 849, 973, 1042, 1102, 1229, 1246, 1405
BtsI GCAGTG 2 cut(s) 1563, 1575
BtsIMutI CAGTG 7 cut(s) 616, 782, 805, 1563, 1575, 1685, 1707
Cac8I GCNNGC 1 cut(s) 616
CaiI CAGNNNCTG 2 cut(s) 47, 1268
CciI TCATGA 1 cut(s) 352
CfoI GCGC 2 cut(s) 90, 276
Csp6I GTAC 3 cut(s) 4, 773, 990
CviQI GTAC 3 cut(s) 4, 773, 990
DdeI CTNAG 8 cut(s) 69, 404, 481, 506, 797, 1200, 1367, 1483
DpnI GATC 7 cut(s) 123, 372, 866, 1053, 1141, 1620, 1698
DpnII GATC 7 cut(s) 121, 370, 864, 1051, 1139, 1618, 1696
Eam1104I CTCTTC 3 cut(s) 393, 552, 1542
EarI CTCTTC 3 cut(s) 393, 552, 1542
Eco31I GGTCTC 3 cut(s) 649, 1486, 1552
Eco57I CTGAAG 3 cut(s) 272, 663, 1038
Eco88I CYCGRG 1 cut(s) 855
EcoRII CCWGG 1 cut(s) 318
EcoT22I ATGCAT 2 cut(s) 540, 985
Esp3I CGTCTC 1 cut(s) 1487
FalI AAGNNNNNCTT 2 cut(s) 464, 496
FauNDI CATATG 1 cut(s) 165
FblI GTMKAC 1 cut(s) 328
Fnu4HI GCNGC 3 cut(s) 225, 1260, 1683
FokI GGATG 8 cut(s) 508, 856, 980, 1049, 1109, 1236, 1253, 1412
Fsp4HI GCNGC 3 cut(s) 225, 1260, 1683
FspBI CTAG 7 cut(s) 108, 194, 650, 827, 1004, 1041, 1449
GlaI GCGC 2 cut(s) 89, 275
GluI GCNGC 3 cut(s) 225, 1260, 1683
GsuI CTGGAG 1 cut(s) 21
HaeII RGCGCY 1 cut(s) 277
HaeIII GGCC 1 cut(s) 1205
HapII CCGG 1 cut(s) 1186
HhaI GCGC 2 cut(s) 90, 276
Hin6I GCGC 2 cut(s) 88, 274
HinP1I GCGC 2 cut(s) 88, 274
HincII GTYRAC 1 cut(s) 1347
HindII GTYRAC 1 cut(s) 1347
HindIII AAGCTT 3 cut(s) 478, 508, 720
HinfI GANTC 4 cut(s) 50, 1177, 1265, 1526
HpaII CCGG 1 cut(s) 1186
HphI GGTGA 5 cut(s) 380, 476, 928, 959, 1649
Hpy166II GTNNAC 3 cut(s) 329, 782, 1347
Hpy188I TCNGA 4 cut(s) 407, 1010, 1486, 1712
Hpy188III TCNNGA 8 cut(s) 344, 353, 566, 589, 857, 922, 1027, 1094
Hpy8I GTNNAC 3 cut(s) 329, 782, 1347
HpyAV CCTTC 4 cut(s) 199, 1214, 1528, 1654
HpyCH4III ACNGT 4 cut(s) 464, 777, 961, 1689
HpyCH4IV ACGT 2 cut(s) 325, 915
HpyF10VI GCNNNNNNNGC 2 cut(s) 38, 137
HpyF3I CTNAG 8 cut(s) 69, 404, 481, 506, 797, 1200, 1367, 1483
HpySE526I ACGT 2 cut(s) 325, 915
HspAI GCGC 2 cut(s) 88, 274
Kzo9I GATC 7 cut(s) 121, 370, 864, 1051, 1139, 1618, 1696
LguI GCTCTTC 1 cut(s) 393
LmnI GCTCC 1 cut(s) 184
Lsp1109I GCAGC 2 cut(s) 211, 1669
LweI GCATC 9 cut(s) 253, 445, 525, 530, 834, 970, 1027, 1214, 1630
MaeI CTAG 7 cut(s) 108, 194, 650, 827, 1004, 1041, 1449
MaeII ACGT 2 cut(s) 325, 915
MaeIII GTNAC 7 cut(s) 464, 484, 695, 738, 800, 955, 1274
MalI GATC 7 cut(s) 123, 372, 866, 1053, 1141, 1620, 1698
MboI GATC 7 cut(s) 121, 370, 864, 1051, 1139, 1618, 1696
MboII GAAGA 6 cut(s) 380, 569, 1010, 1149, 1186, 1559
MhlI GDGCHC 3 cut(s) 691, 1416, 1707
MluCI AATT 8 cut(s) 97, 133, 258, 548, 766, 1121, 1167, 1518
MmeI TCCRAC 1 cut(s) 1636
Mph1103I ATGCAT 2 cut(s) 540, 985
MroXI GAANNNNTTC 1 cut(s) 81
MseI TTAA 6 cut(s) 605, 765, 1013, 1509, 1587, 1599
MslI CAYNNNNRTG 4 cut(s) 357, 1339, 1568, 1610
MspI CCGG 1 cut(s) 1186
MspR9I CCNGG 2 cut(s) 320, 1186
MvaI CCWGG 1 cut(s) 320
MwoI GCNNNNNNNGC 2 cut(s) 38, 137
NciI CCSGG 1 cut(s) 1186
NdeI CATATG 1 cut(s) 165
NdeII GATC 7 cut(s) 121, 370, 864, 1051, 1139, 1618, 1696
NlaIV GGNNCC 1 cut(s) 1214
NmeAIII GCCGAG 1 cut(s) 670
NmuCI GTSAC 2 cut(s) 464, 800
NsiI ATGCAT 2 cut(s) 540, 985
NspI RCATGY 4 cut(s) 335, 703, 1442, 1655
NspV TTCGAA 1 cut(s) 1376
OliI CACNNNNGTG 2 cut(s) 1568, 1610
PaeR7I CTCGAG 1 cut(s) 855
PagI TCATGA 1 cut(s) 352
PciI ACATGT 1 cut(s) 699
PciSI GCTCTTC 1 cut(s) 393
PdmI GAANNNNTTC 1 cut(s) 81
PfeI GAWTC 4 cut(s) 50, 1177, 1265, 1526
PkrI GCNGC 3 cut(s) 226, 1261, 1684
Ppu21I YACGTR 1 cut(s) 916
PscI ACATGT 1 cut(s) 699
PsiI TTATAA 2 cut(s) 152, 1322
Psp6I CCWGG 1 cut(s) 318
PspGI CCWGG 1 cut(s) 318
PspN4I GGNNCC 1 cut(s) 1214
PstI CTGCAG 1 cut(s) 1570
PstNI CAGNNNCTG 2 cut(s) 47, 1268
RsaI GTAC 3 cut(s) 5, 774, 991
RsaNI GTAC 3 cut(s) 4, 773, 990
RseI CAYNNNNRTG 4 cut(s) 357, 1339, 1568, 1610
SapI GCTCTTC 1 cut(s) 393
SaqAI TTAA 6 cut(s) 605, 765, 1013, 1509, 1587, 1599
SatI GCNGC 3 cut(s) 225, 1260, 1683
Sau3AI GATC 7 cut(s) 121, 370, 864, 1051, 1139, 1618, 1696
ScrFI CCNGG 2 cut(s) 320, 1186
SduI GDGCHC 3 cut(s) 691, 1416, 1707
SfaNI GCATC 9 cut(s) 253, 445, 525, 530, 834, 970, 1027, 1214, 1630
SfcI CTRYAG 2 cut(s) 672, 1566
Sfr274I CTCGAG 1 cut(s) 855
SfuI TTCGAA 1 cut(s) 1376
SlaI CTCGAG 1 cut(s) 855
SmiMI CAYNNNNRTG 4 cut(s) 357, 1339, 1568, 1610
SmlI CTYRAG 4 cut(s) 156, 587, 855, 1608
SmoI CTYRAG 4 cut(s) 156, 587, 855, 1608
SpeI ACTAGT 2 cut(s) 193, 1003
Sse9I AATT 8 cut(s) 97, 133, 258, 548, 766, 1121, 1167, 1518
SsiI CCGC 2 cut(s) 1259, 1523
SspMI CTAG 7 cut(s) 108, 194, 650, 827, 1004, 1041, 1449
StyD4I CCNGG 2 cut(s) 318, 1184
TaaI ACNGT 4 cut(s) 464, 777, 961, 1689
TaiI ACGT 2 cut(s) 328, 918
TaqI TCGA 5 cut(s) 397, 567, 856, 1026, 1376
TasI AATT 8 cut(s) 97, 133, 258, 548, 766, 1121, 1167, 1518
TatI WGTACW 1 cut(s) 772
TauI GCSGC 1 cut(s) 1262
TfiI GAWTC 4 cut(s) 50, 1177, 1265, 1526
Tru1I TTAA 6 cut(s) 605, 765, 1013, 1509, 1587, 1599
Tru9I TTAA 6 cut(s) 605, 765, 1013, 1509, 1587, 1599
TscAI CASTG 7 cut(s) 616, 782, 805, 1570, 1575, 1692, 1707
TseFI GTSAC 2 cut(s) 464, 800
TseI GCWGC 2 cut(s) 224, 1682
Tsp45I GTSAC 2 cut(s) 464, 800
TspDTI ATGAA 8 cut(s) 341, 732, 1122, 1187, 1481, 1485, 1560, 1682
TspRI CASTG 7 cut(s) 616, 782, 805, 1570, 1575, 1692, 1707
XapI RAATTY 1 cut(s) 97
XceI RCATGY 4 cut(s) 335, 703, 1442, 1655
XhoI CTCGAG 1 cut(s) 855
XmiI GTMKAC 1 cut(s) 328
XmnI GAANNNNTTC 1 cut(s) 81
XspI CTAG 7 cut(s) 108, 194, 650, 827, 1004, 1041, 1449
Zsp2I ATGCAT 2 cut(s) 540, 985
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.