(-)-germacrene D synthase-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 171 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 131 |
| AatII | GACGTC | 1 cut(s) | 129 |
| AccB1I | GGYRCC | 1 cut(s) | 566 |
| AccI | GTMKAC | 1 cut(s) | 105 |
| AciI | CCGC | 2 cut(s) | 20, 497 |
| AcoI | YGGCCR | 1 cut(s) | 435 |
| AcyI | GRCGYC | 1 cut(s) | 126 |
| AfaI | GTAC | 2 cut(s) | 245, 722 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 429, 484 |
| AflII | CTTAAG | 1 cut(s) | 758 |
| AgsI | TTSAA | 4 cut(s) | 99, 203, 275, 290 |
| AluBI | AGCT | 5 cut(s) | 89, 211, 268, 293, 539 |
| AluI | AGCT | 5 cut(s) | 89, 211, 268, 293, 539 |
| AoxI | GGCC | 2 cut(s) | 435, 665 |
| ApeKI | GCWGC | 1 cut(s) | 464 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 491, 523 |
| AseI | ATTAAT | 1 cut(s) | 350 |
| AspS9I | GGNCC | 1 cut(s) | 499 |
| AvaII | GGWCC | 1 cut(s) | 499 |
| BalI | TGGCCA | 1 cut(s) | 437 |
| BanI | GGYRCC | 1 cut(s) | 566 |
| BbvCI | CCTCAGC | 1 cut(s) | 535 |
| BbvI | GCAGC | 1 cut(s) | 451 |
| BccI | CCATC | 1 cut(s) | 722 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 652, 686 |
| BciVI | GTATCC | 1 cut(s) | 27 |
| BfaI | CTAG | 1 cut(s) | 344 |
| BfmI | CTRYAG | 2 cut(s) | 465, 540 |
| BfrI | CTTAAG | 1 cut(s) | 758 |
| BfuI | GTATCC | 1 cut(s) | 27 |
| BglII | AGATCT | 1 cut(s) | 139 |
| BisI | GCNGC | 1 cut(s) | 465 |
| BlsI | GCNGC | 1 cut(s) | 466 |
| Bme18I | GGWCC | 1 cut(s) | 499 |
| BmgT120I | GGNCC | 1 cut(s) | 499 |
| BmiI | GGNNCC | 1 cut(s) | 568 |
| Bpu10I | CCTNAGC | 1 cut(s) | 535 |
| BsaHI | GRCGYC | 1 cut(s) | 126 |
| BsaJI | CCNNGG | 2 cut(s) | 447, 662 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 429, 484 |
| Bse1I | ACTGG | 1 cut(s) | 241 |
| Bse3DI | GCAATG | 1 cut(s) | 668 |
| BseDI | CCNNGG | 2 cut(s) | 447, 662 |
| BseGI | GGATG | 3 cut(s) | 334, 493, 712 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 429, 484 |
| BseMI | GCAATG | 1 cut(s) | 668 |
| BseMII | CTCAG | 1 cut(s) | 549 |
| BseNI | ACTGG | 1 cut(s) | 241 |
| BseRI | GAGGAG | 1 cut(s) | 635 |
| BseXI | GCAGC | 1 cut(s) | 451 |
| BshFI | GGCC | 2 cut(s) | 437, 667 |
| BshNI | GGYRCC | 1 cut(s) | 566 |
| BslFI | GGGAC | 2 cut(s) | 137, 325 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 429, 484 |
| BsmFI | GGGAC | 2 cut(s) | 137, 325 |
| BsnI | GGCC | 2 cut(s) | 437, 667 |
| Bsp143I | GATC | 1 cut(s) | 139 |
| BspACI | CCGC | 2 cut(s) | 20, 497 |
| BspANI | GGCC | 2 cut(s) | 437, 667 |
| BspCNI | CTCAG | 1 cut(s) | 548 |
| BspHI | TCATGA | 1 cut(s) | 745 |
| BspLI | GGNNCC | 1 cut(s) | 568 |
| BspMAI | CTGCAG | 1 cut(s) | 469 |
| BspT107I | GGYRCC | 1 cut(s) | 566 |
| BspTI | CTTAAG | 1 cut(s) | 758 |
| BsrDI | GCAATG | 1 cut(s) | 668 |
| BsrI | ACTGG | 1 cut(s) | 241 |
| BssECI | CCNNGG | 2 cut(s) | 447, 662 |
| BssMI | GATC | 1 cut(s) | 139 |
| BssNI | GRCGYC | 1 cut(s) | 126 |
| BssT1I | CCWWGG | 1 cut(s) | 447 |
| Bst4CI | ACNGT | 2 cut(s) | 180, 754 |
| BstACI | GRCGYC | 1 cut(s) | 126 |
| BstAFI | CTTAAG | 1 cut(s) | 758 |
| BstC8I | GCNNGC | 1 cut(s) | 213 |
| BstDEI | CTNAG | 1 cut(s) | 535 |
| BstF5I | GGATG | 3 cut(s) | 334, 493, 712 |
| BstKTI | GATC | 1 cut(s) | 142 |
| BstMBI | GATC | 1 cut(s) | 139 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 310 |
| BstNSI | RCATGY | 1 cut(s) | 699 |
| BstSFI | CTRYAG | 2 cut(s) | 465, 540 |
| BstV1I | GCAGC | 1 cut(s) | 451 |
| BstX2I | RGATCY | 1 cut(s) | 139 |
| BstYI | RGATCY | 1 cut(s) | 139 |
| BsuI | GTATCC | 1 cut(s) | 27 |
| BsuRI | GGCC | 2 cut(s) | 437, 667 |
| BtsCI | GGATG | 3 cut(s) | 334, 493, 712 |
| BtsIMutI | CAGTG | 1 cut(s) | 176 |
| Cac8I | GCNNGC | 1 cut(s) | 213 |
| CciI | TCATGA | 1 cut(s) | 745 |
| Cfr13I | GGNCC | 1 cut(s) | 499 |
| CseI | GACGC | 1 cut(s) | 623 |
| Csp6I | GTAC | 2 cut(s) | 244, 721 |
| CviAII | CATG | 6 cut(s) | 484, 553, 618, 696, 703, 746 |
| CviQI | GTAC | 2 cut(s) | 244, 721 |
| DdeI | CTNAG | 1 cut(s) | 535 |
| DpnI | GATC | 1 cut(s) | 141 |
| DpnII | GATC | 1 cut(s) | 139 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 131 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 131 |
| EaeI | YGGCCR | 1 cut(s) | 435 |
| Eco130I | CCWWGG | 1 cut(s) | 447 |
| Eco47I | GGWCC | 1 cut(s) | 499 |
| EcoT14I | CCWWGG | 1 cut(s) | 447 |
| ErhI | CCWWGG | 1 cut(s) | 447 |
| FaeI | CATG | 6 cut(s) | 487, 556, 621, 699, 706, 749 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 195, 227 |
| FaqI | GGGAC | 2 cut(s) | 137, 325 |
| FatI | CATG | 6 cut(s) | 483, 552, 617, 695, 702, 745 |
| FblI | GTMKAC | 1 cut(s) | 105 |
| Fnu4HI | GCNGC | 1 cut(s) | 465 |
| FokI | GGATG | 3 cut(s) | 341, 500, 719 |
| Fsp4HI | GCNGC | 1 cut(s) | 465 |
| FspBI | CTAG | 1 cut(s) | 344 |
| GluI | GCNGC | 1 cut(s) | 465 |
| HaeIII | GGCC | 2 cut(s) | 437, 667 |
| HgaI | GACGC | 1 cut(s) | 623 |
| Hin1I | GRCGYC | 1 cut(s) | 126 |
| Hin1II | CATG | 6 cut(s) | 487, 556, 621, 699, 706, 749 |
| HinfI | GANTC | 3 cut(s) | 419, 480, 635 |
| Hpy166II | GTNNAC | 1 cut(s) | 106 |
| Hpy188I | TCNGA | 2 cut(s) | 593, 663 |
| Hpy188III | TCNNGA | 1 cut(s) | 746 |
| Hpy8I | GTNNAC | 1 cut(s) | 106 |
| HpyAV | CCTTC | 3 cut(s) | 215, 433, 616 |
| HpyCH4III | ACNGT | 2 cut(s) | 180, 754 |
| HpyCH4IV | ACGT | 1 cut(s) | 126 |
| HpyCH4V | TGCA | 6 cut(s) | 84, 215, 304, 467, 695, 713 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 310 |
| HpyF3I | CTNAG | 1 cut(s) | 535 |
| HpySE526I | ACGT | 1 cut(s) | 126 |
| Hsp92I | GRCGYC | 1 cut(s) | 126 |
| Hsp92II | CATG | 6 cut(s) | 487, 556, 621, 699, 706, 749 |
| Kzo9I | GATC | 1 cut(s) | 139 |
| LmnI | GCTCC | 1 cut(s) | 664 |
| LpnPI | CCDG | 6 cut(s) | 162, 254, 262, 320, 345, 383 |
| Lsp1109I | GCAGC | 1 cut(s) | 451 |
| MaeI | CTAG | 1 cut(s) | 344 |
| MaeII | ACGT | 1 cut(s) | 126 |
| MaeIII | GTNAC | 4 cut(s) | 144, 529, 611, 731 |
| MalI | GATC | 1 cut(s) | 141 |
| MboI | GATC | 1 cut(s) | 139 |
| MboII | GAAGA | 3 cut(s) | 215, 587, 590 |
| MfeI | CAATTG | 1 cut(s) | 99 |
| MflI | RGATCY | 1 cut(s) | 139 |
| MlsI | TGGCCA | 1 cut(s) | 437 |
| MluCI | AATT | 6 cut(s) | 99, 198, 227, 277, 471, 681 |
| MluNI | TGGCCA | 1 cut(s) | 437 |
| MlyI | GAGTC | 2 cut(s) | 413, 474 |
| MmeI | TCCRAC | 1 cut(s) | 373 |
| MnlI | CCTC | 5 cut(s) | 515, 544, 653, 656, 657 |
| Mox20I | TGGCCA | 1 cut(s) | 437 |
| MscI | TGGCCA | 1 cut(s) | 437 |
| MseI | TTAA | 5 cut(s) | 54, 75, 350, 687, 759 |
| Msp20I | TGGCCA | 1 cut(s) | 437 |
| MspA1I | CMGCKG | 1 cut(s) | 539 |
| MspCI | CTTAAG | 1 cut(s) | 758 |
| MunI | CAATTG | 1 cut(s) | 99 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 310 |
| NdeII | GATC | 1 cut(s) | 139 |
| NlaIII | CATG | 6 cut(s) | 487, 556, 621, 699, 706, 749 |
| NlaIV | GGNNCC | 1 cut(s) | 568 |
| NmuCI | GTSAC | 1 cut(s) | 611 |
| NspI | RCATGY | 1 cut(s) | 699 |
| PagI | TCATGA | 1 cut(s) | 745 |
| PfeI | GAWTC | 1 cut(s) | 635 |
| PkrI | GCNGC | 1 cut(s) | 466 |
| PleI | GAGTC | 2 cut(s) | 413, 474 |
| PpsI | GAGTC | 2 cut(s) | 413, 474 |
| PshBI | ATTAAT | 1 cut(s) | 350 |
| PsiI | TTATAA | 1 cut(s) | 171 |
| PspN4I | GGNNCC | 1 cut(s) | 568 |
| PspPI | GGNCC | 1 cut(s) | 499 |
| PstI | CTGCAG | 1 cut(s) | 469 |
| PsuI | RGATCY | 1 cut(s) | 139 |
| PvuII | CAGCTG | 1 cut(s) | 539 |
| RsaI | GTAC | 2 cut(s) | 245, 722 |
| RsaNI | GTAC | 2 cut(s) | 244, 721 |
| SaqAI | TTAA | 5 cut(s) | 54, 75, 350, 687, 759 |
| SatI | GCNGC | 1 cut(s) | 465 |
| Sau3AI | GATC | 1 cut(s) | 139 |
| Sau96I | GGNCC | 1 cut(s) | 499 |
| SchI | GAGTC | 2 cut(s) | 413, 474 |
| SfcI | CTRYAG | 2 cut(s) | 465, 540 |
| SinI | GGWCC | 1 cut(s) | 499 |
| SmlI | CTYRAG | 1 cut(s) | 758 |
| SmoI | CTYRAG | 1 cut(s) | 758 |
| Sse9I | AATT | 6 cut(s) | 99, 198, 227, 277, 471, 681 |
| SsiI | CCGC | 2 cut(s) | 20, 497 |
| SspI | AATATT | 1 cut(s) | 406 |
| SspMI | CTAG | 1 cut(s) | 344 |
| StyI | CCWWGG | 1 cut(s) | 447 |
| TaaI | ACNGT | 2 cut(s) | 180, 754 |
| TaiI | ACGT | 1 cut(s) | 129 |
| TaqI | TCGA | 2 cut(s) | 426, 679 |
| TasI | AATT | 6 cut(s) | 99, 198, 227, 277, 471, 681 |
| TfiI | GAWTC | 1 cut(s) | 635 |
| Tru1I | TTAA | 5 cut(s) | 54, 75, 350, 687, 759 |
| Tru9I | TTAA | 5 cut(s) | 54, 75, 350, 687, 759 |
| TscAI | CASTG | 1 cut(s) | 183 |
| TseFI | GTSAC | 1 cut(s) | 611 |
| TseI | GCWGC | 1 cut(s) | 464 |
| Tsp45I | GTSAC | 1 cut(s) | 611 |
| TspDTI | ATGAA | 4 cut(s) | 173, 275, 569, 648 |
| TspRI | CASTG | 1 cut(s) | 183 |
| Vha464I | CTTAAG | 1 cut(s) | 758 |
| VpaK11BI | GGWCC | 1 cut(s) | 499 |
| VspI | ATTAAT | 1 cut(s) | 350 |
| XceI | RCATGY | 1 cut(s) | 699 |
| XmiI | GTMKAC | 1 cut(s) | 105 |
| XspI | CTAG | 1 cut(s) | 344 |
| ZraI | GACGTC | 1 cut(s) | 127 |