synthase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 1502, 1534 |
| Acc16I | TGCGCA | 1 cut(s) | 954 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 99 |
| AccI | GTMKAC | 3 cut(s) | 531, 702, 726 |
| AciI | CCGC | 3 cut(s) | 482, 1042, 1232 |
| AclWI | GGATC | 1 cut(s) | 307 |
| AcsI | RAATTY | 2 cut(s) | 289, 936 |
| AcuI | CTGAAG | 2 cut(s) | 60, 864 |
| AdeI | CACNNNGTG | 1 cut(s) | 639 |
| AfaI | GTAC | 2 cut(s) | 162, 1451 |
| AfeI | AGCGCT | 1 cut(s) | 251 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 99, 753, 1164 |
| AflIII | ACRYGT | 2 cut(s) | 157, 1074 |
| AgeI | ACCGGT | 1 cut(s) | 1094 |
| AhlI | ACTAGT | 1 cut(s) | 1513 |
| AjiI | CACGTC | 1 cut(s) | 658 |
| AjnI | CCWGG | 1 cut(s) | 534 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 572, 604 |
| AluBI | AGCT | 7 cut(s) | 226, 333, 835, 848, 949, 1145, 1529 |
| AluI | AGCT | 7 cut(s) | 226, 333, 835, 848, 949, 1145, 1529 |
| Alw21I | GWGCWC | 1 cut(s) | 837 |
| Alw26I | GTCTC | 2 cut(s) | 678, 871 |
| AlwI | GGATC | 1 cut(s) | 307 |
| Ama87I | CYCGRG | 1 cut(s) | 1431 |
| Aor51HI | AGCGCT | 1 cut(s) | 251 |
| AoxI | GGCC | 2 cut(s) | 65, 1302 |
| ApeKI | GCWGC | 1 cut(s) | 1101 |
| ApoI | RAATTY | 2 cut(s) | 289, 936 |
| AsiGI | ACCGGT | 1 cut(s) | 1094 |
| AspLEI | GCGC | 2 cut(s) | 252, 955 |
| AspS9I | GGNCC | 1 cut(s) | 1302 |
| AsuHPI | GGTGA | 2 cut(s) | 116, 543 |
| AsuII | TTCGAA | 1 cut(s) | 487 |
| AvaI | CYCGRG | 1 cut(s) | 1431 |
| AxyI | CCTNAGG | 1 cut(s) | 353 |
| BanII | GRGCYC | 1 cut(s) | 837 |
| BauI | CACGAG | 1 cut(s) | 1088 |
| Bbv12I | GWGCWC | 1 cut(s) | 837 |
| BbvI | GCAGC | 1 cut(s) | 1088 |
| BccI | CCATC | 4 cut(s) | 618, 953, 1066, 1457 |
| BciT130I | CCWGG | 1 cut(s) | 536 |
| BciVI | GTATCC | 2 cut(s) | 351, 1460 |
| BcoDI | GTCTC | 2 cut(s) | 678, 871 |
| BcuI | ACTAGT | 1 cut(s) | 1513 |
| BfaI | CTAG | 3 cut(s) | 377, 753, 1514 |
| BfmI | CTRYAG | 1 cut(s) | 1524 |
| BfoI | RGCGCY | 1 cut(s) | 253 |
| BfuI | GTATCC | 2 cut(s) | 351, 1460 |
| BglII | AGATCT | 1 cut(s) | 1495 |
| BisI | GCNGC | 2 cut(s) | 482, 1102 |
| BlsI | GCNGC | 2 cut(s) | 483, 1103 |
| Bme1390I | CCNGG | 1 cut(s) | 536 |
| BmeT110I | CYCGRG | 1 cut(s) | 1431 |
| BmgBI | CACGTC | 1 cut(s) | 658 |
| BmgT120I | GGNCC | 1 cut(s) | 1302 |
| BmiI | GGNNCC | 1 cut(s) | 1303 |
| BmrFI | CCNGG | 1 cut(s) | 536 |
| BmsI | GCATC | 2 cut(s) | 130, 984 |
| BpmI | CTGGAG | 2 cut(s) | 851, 1167 |
| Bpu10I | CCTNAGC | 2 cut(s) | 69, 1479 |
| Bpu14I | TTCGAA | 1 cut(s) | 487 |
| Bsa29I | ATCGAT | 1 cut(s) | 795 |
| BsaBI | GATNNNNATC | 2 cut(s) | 28, 60 |
| BsaJI | CCNNGG | 1 cut(s) | 500 |
| BsaWI | WCCGGW | 1 cut(s) | 1094 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1165, 1195 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 99, 753, 1164 |
| Bse118I | RCCGGY | 1 cut(s) | 1094 |
| Bse1I | ACTGG | 4 cut(s) | 696, 834, 1068, 1184 |
| Bse21I | CCTNAGG | 1 cut(s) | 353 |
| Bse8I | GATNNNNATC | 2 cut(s) | 28, 60 |
| BseBI | CCWGG | 1 cut(s) | 536 |
| BseCI | ATCGAT | 1 cut(s) | 795 |
| BseDI | CCNNGG | 1 cut(s) | 500 |
| BseGI | GGATG | 6 cut(s) | 145, 975, 1072, 1121, 1228, 1447 |
| BseJI | GATNNNNATC | 2 cut(s) | 28, 60 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 99, 753, 1164 |
| BseMII | CTCAG | 2 cut(s) | 882, 1225 |
| BseNI | ACTGG | 4 cut(s) | 696, 834, 1068, 1184 |
| BseRI | GAGGAG | 1 cut(s) | 383 |
| BseXI | GCAGC | 1 cut(s) | 1088 |
| BseYI | CCCAGC | 2 cut(s) | 92, 711 |
| BshFI | GGCC | 2 cut(s) | 67, 1304 |
| BshTI | ACCGGT | 1 cut(s) | 1094 |
| BshVI | ATCGAT | 1 cut(s) | 795 |
| BsiHKAI | GWGCWC | 1 cut(s) | 837 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1431 |
| BsiSI | CCGG | 1 cut(s) | 1095 |
| BslFI | GGGAC | 1 cut(s) | 1379 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 99, 753, 1164 |
| BsmAI | GTCTC | 2 cut(s) | 678, 871 |
| BsmFI | GGGAC | 1 cut(s) | 1379 |
| BsmI | GAATGC | 1 cut(s) | 195 |
| BsnI | GGCC | 2 cut(s) | 67, 1304 |
| BsoBI | CYCGRG | 1 cut(s) | 1431 |
| Bsp119I | TTCGAA | 1 cut(s) | 487 |
| Bsp1286I | GDGCHC | 1 cut(s) | 837 |
| Bsp1407I | TGTACA | 2 cut(s) | 160, 1449 |
| Bsp143I | GATC | 4 cut(s) | 23, 299, 877, 1495 |
| BspACI | CCGC | 3 cut(s) | 482, 1042, 1232 |
| BspANI | GGCC | 2 cut(s) | 67, 1304 |
| BspCNI | CTCAG | 2 cut(s) | 881, 1224 |
| BspDI | ATCGAT | 1 cut(s) | 795 |
| BspHI | TCATGA | 1 cut(s) | 940 |
| BspLI | GGNNCC | 1 cut(s) | 1303 |
| BspPI | GGATC | 1 cut(s) | 307 |
| BspT104I | TTCGAA | 1 cut(s) | 487 |
| BsrFI | RCCGGY | 1 cut(s) | 1094 |
| BsrGI | TGTACA | 2 cut(s) | 160, 1449 |
| BsrI | ACTGG | 4 cut(s) | 696, 834, 1068, 1184 |
| BssAI | RCCGGY | 1 cut(s) | 1094 |
| BssECI | CCNNGG | 1 cut(s) | 500 |
| BssMI | GATC | 4 cut(s) | 23, 299, 877, 1495 |
| BssSI | CACGAG | 1 cut(s) | 1088 |
| BssT1I | CCWWGG | 1 cut(s) | 500 |
| Bst2BI | CACGAG | 1 cut(s) | 1088 |
| Bst2UI | CCWGG | 1 cut(s) | 536 |
| Bst4CI | ACNGT | 3 cut(s) | 281, 724, 1283 |
| BstAUI | TGTACA | 2 cut(s) | 160, 1449 |
| BstBI | TTCGAA | 1 cut(s) | 487 |
| BstC8I | GCNNGC | 2 cut(s) | 951, 1375 |
| BstDEI | CTNAG | 9 cut(s) | 69, 253, 329, 353, 542, 623, 868, 1211, 1479 |
| BstEII | GGTNACC | 1 cut(s) | 104 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 751 |
| BstF5I | GGATG | 6 cut(s) | 145, 975, 1072, 1121, 1228, 1447 |
| BstH2I | RGCGCY | 1 cut(s) | 253 |
| BstHHI | GCGC | 2 cut(s) | 252, 955 |
| BstKTI | GATC | 4 cut(s) | 26, 302, 880, 1498 |
| BstMAI | GTCTC | 2 cut(s) | 678, 871 |
| BstMBI | GATC | 4 cut(s) | 23, 299, 877, 1495 |
| BstNI | CCWGG | 1 cut(s) | 536 |
| BstNSI | RCATGY | 3 cut(s) | 161, 1057, 1078 |
| BstPI | GGTNACC | 1 cut(s) | 104 |
| BstSCI | CCNGG | 1 cut(s) | 534 |
| BstSFI | CTRYAG | 1 cut(s) | 1524 |
| BstV1I | GCAGC | 1 cut(s) | 1088 |
| BstX2I | RGATCY | 1 cut(s) | 1495 |
| BstYI | RGATCY | 1 cut(s) | 1495 |
| Bsu15I | ATCGAT | 1 cut(s) | 795 |
| Bsu36I | CCTNAGG | 1 cut(s) | 353 |
| BsuI | GTATCC | 2 cut(s) | 351, 1460 |
| BsuRI | GGCC | 2 cut(s) | 67, 1304 |
| BsuTUI | ATCGAT | 1 cut(s) | 795 |
| BtrI | CACGTC | 1 cut(s) | 658 |
| BtsCI | GGATG | 6 cut(s) | 145, 975, 1072, 1121, 1228, 1447 |
| BtsIMutI | CAGTG | 1 cut(s) | 1061 |
| Cac8I | GCNNGC | 2 cut(s) | 951, 1375 |
| CciI | TCATGA | 1 cut(s) | 940 |
| CfoI | GCGC | 2 cut(s) | 252, 955 |
| Cfr10I | RCCGGY | 1 cut(s) | 1094 |
| Cfr13I | GGNCC | 1 cut(s) | 1302 |
| ClaI | ATCGAT | 1 cut(s) | 795 |
| Csp6I | GTAC | 2 cut(s) | 161, 1450 |
| CspAI | ACCGGT | 1 cut(s) | 1094 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 430, 465, 1108, 1143 |
| CviQI | GTAC | 2 cut(s) | 161, 1450 |
| DdeI | CTNAG | 9 cut(s) | 69, 253, 329, 353, 542, 623, 868, 1211, 1479 |
| DpnI | GATC | 4 cut(s) | 25, 301, 879, 1497 |
| DpnII | GATC | 4 cut(s) | 23, 299, 877, 1495 |
| DraIII | CACNNNGTG | 1 cut(s) | 639 |
| EciI | GGCGGA | 1 cut(s) | 1031 |
| Ecl136II | GAGCTC | 1 cut(s) | 835 |
| Eco130I | CCWWGG | 1 cut(s) | 500 |
| Eco24I | GRGCYC | 1 cut(s) | 837 |
| Eco47III | AGCGCT | 1 cut(s) | 251 |
| Eco53kI | GAGCTC | 1 cut(s) | 835 |
| Eco57I | CTGAAG | 2 cut(s) | 60, 864 |
| Eco81I | CCTNAGG | 1 cut(s) | 353 |
| Eco88I | CYCGRG | 1 cut(s) | 1431 |
| Eco91I | GGTNACC | 1 cut(s) | 104 |
| EcoICRI | GAGCTC | 1 cut(s) | 835 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 751 |
| EcoO65I | GGTNACC | 1 cut(s) | 104 |
| EcoRII | CCWGG | 1 cut(s) | 534 |
| EcoT14I | CCWWGG | 1 cut(s) | 500 |
| EcoT22I | ATGCAT | 1 cut(s) | 1354 |
| EcoT38I | GRGCYC | 1 cut(s) | 837 |
| ErhI | CCWWGG | 1 cut(s) | 500 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 215, 247 |
| FaqI | GGGAC | 1 cut(s) | 1379 |
| FblI | GTMKAC | 3 cut(s) | 531, 702, 726 |
| Fnu4HI | GCNGC | 2 cut(s) | 482, 1102 |
| FokI | GGATG | 6 cut(s) | 152, 962, 1079, 1108, 1235, 1454 |
| FriOI | GRGCYC | 1 cut(s) | 837 |
| Fsp4HI | GCNGC | 2 cut(s) | 482, 1102 |
| FspBI | CTAG | 3 cut(s) | 377, 753, 1514 |
| FspI | TGCGCA | 1 cut(s) | 954 |
| GlaI | GCGC | 2 cut(s) | 251, 954 |
| GluI | GCNGC | 2 cut(s) | 482, 1102 |
| GsaI | CCCAGC | 2 cut(s) | 96, 715 |
| GsuI | CTGGAG | 2 cut(s) | 851, 1167 |
| HaeII | RGCGCY | 1 cut(s) | 253 |
| HaeIII | GGCC | 2 cut(s) | 67, 1304 |
| HapII | CCGG | 1 cut(s) | 1095 |
| HhaI | GCGC | 2 cut(s) | 252, 955 |
| Hin6I | GCGC | 2 cut(s) | 250, 953 |
| HinP1I | GCGC | 2 cut(s) | 250, 953 |
| HincII | GTYRAC | 2 cut(s) | 727, 1203 |
| HindII | GTYRAC | 2 cut(s) | 727, 1203 |
| HindIII | AAGCTT | 2 cut(s) | 331, 947 |
| HinfI | GANTC | 6 cut(s) | 125, 413, 548, 606, 1154, 1215 |
| HpaII | CCGG | 1 cut(s) | 1095 |
| HphI | GGTGA | 2 cut(s) | 116, 543 |
| Hpy166II | GTNNAC | 7 cut(s) | 497, 532, 703, 727, 1203, 1279, 1450 |
| Hpy188I | TCNGA | 2 cut(s) | 615, 1214 |
| Hpy188III | TCNNGA | 3 cut(s) | 410, 941, 1090 |
| Hpy8I | GTNNAC | 7 cut(s) | 497, 532, 703, 727, 1203, 1279, 1450 |
| HpyAV | CCTTC | 6 cut(s) | 84, 166, 403, 640, 1186, 1351 |
| HpyCH4III | ACNGT | 3 cut(s) | 281, 724, 1283 |
| HpyCH4IV | ACGT | 3 cut(s) | 657, 924, 1199 |
| HpyCH4V | TGCA | 5 cut(s) | 452, 601, 680, 986, 1352 |
| HpyF3I | CTNAG | 9 cut(s) | 69, 253, 329, 353, 542, 623, 868, 1211, 1479 |
| HpySE526I | ACGT | 3 cut(s) | 657, 924, 1199 |
| HspAI | GCGC | 2 cut(s) | 250, 953 |
| Kzo9I | GATC | 4 cut(s) | 23, 299, 877, 1495 |
| LmnI | GCTCC | 1 cut(s) | 832 |
| Lsp1109I | GCAGC | 1 cut(s) | 1088 |
| LweI | GCATC | 2 cut(s) | 130, 984 |
| MaeI | CTAG | 3 cut(s) | 377, 753, 1514 |
| MaeII | ACGT | 3 cut(s) | 657, 924, 1199 |
| MaeIII | GTNAC | 5 cut(s) | 104, 460, 772, 1090, 1283 |
| MalI | GATC | 4 cut(s) | 25, 301, 879, 1497 |
| MboI | GATC | 4 cut(s) | 23, 299, 877, 1495 |
| MboII | GAAGA | 8 cut(s) | 24, 188, 198, 1151, 1303, 1322, 1325, 1472 |
| MflI | RGATCY | 1 cut(s) | 1495 |
| MhlI | GDGCHC | 1 cut(s) | 837 |
| MluCI | AATT | 9 cut(s) | 167, 289, 936, 965, 1034, 1206, 1410, 1423, 1503 |
| MlyI | GAGTC | 3 cut(s) | 615, 1148, 1209 |
| MmeI | TCCRAC | 3 cut(s) | 564, 900, 1111 |
| MnlI | CCTC | 7 cut(s) | 361, 691, 747, 750, 1007, 1191, 1388 |
| Mph1103I | ATGCAT | 1 cut(s) | 1354 |
| MseI | TTAA | 3 cut(s) | 431, 852, 1172 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1461 |
| MspI | CCGG | 1 cut(s) | 1095 |
| MspR9I | CCNGG | 1 cut(s) | 536 |
| Mva1269I | GAATGC | 1 cut(s) | 195 |
| MvaI | CCWGG | 1 cut(s) | 536 |
| NdeII | GATC | 4 cut(s) | 23, 299, 877, 1495 |
| NlaIV | GGNNCC | 1 cut(s) | 1303 |
| NmuCI | GTSAC | 2 cut(s) | 104, 1090 |
| NsbI | TGCGCA | 1 cut(s) | 954 |
| NsiI | ATGCAT | 1 cut(s) | 1354 |
| NspI | RCATGY | 3 cut(s) | 161, 1057, 1078 |
| NspV | TTCGAA | 1 cut(s) | 487 |
| PagI | TCATGA | 1 cut(s) | 940 |
| PciI | ACATGT | 2 cut(s) | 157, 1074 |
| PctI | GAATGC | 1 cut(s) | 195 |
| PfeI | GAWTC | 3 cut(s) | 125, 413, 548 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 99 |
| PinAI | ACCGGT | 1 cut(s) | 1094 |
| PkrI | GCNGC | 2 cut(s) | 483, 1103 |
| PleI | GAGTC | 3 cut(s) | 614, 1148, 1209 |
| PpsI | GAGTC | 3 cut(s) | 614, 1148, 1209 |
| PscI | ACATGT | 2 cut(s) | 157, 1074 |
| PsiI | TTATAA | 2 cut(s) | 1502, 1534 |
| Psp124BI | GAGCTC | 1 cut(s) | 837 |
| Psp6I | CCWGG | 1 cut(s) | 534 |
| PspEI | GGTNACC | 1 cut(s) | 104 |
| PspFI | CCCAGC | 2 cut(s) | 92, 711 |
| PspGI | CCWGG | 1 cut(s) | 534 |
| PspN4I | GGNNCC | 1 cut(s) | 1303 |
| PspPI | GGNCC | 1 cut(s) | 1302 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 144, 176 |
| PsuI | RGATCY | 1 cut(s) | 1495 |
| RsaI | GTAC | 2 cut(s) | 162, 1451 |
| RsaNI | GTAC | 2 cut(s) | 161, 1450 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1461 |
| SacI | GAGCTC | 1 cut(s) | 837 |
| SalI | GTCGAC | 1 cut(s) | 725 |
| SaqAI | TTAA | 3 cut(s) | 431, 852, 1172 |
| SatI | GCNGC | 2 cut(s) | 482, 1102 |
| Sau3AI | GATC | 4 cut(s) | 23, 299, 877, 1495 |
| Sau96I | GGNCC | 1 cut(s) | 1302 |
| SchI | GAGTC | 3 cut(s) | 615, 1148, 1209 |
| ScrFI | CCNGG | 1 cut(s) | 536 |
| SduI | GDGCHC | 1 cut(s) | 837 |
| SfaNI | GCATC | 2 cut(s) | 130, 984 |
| SfcI | CTRYAG | 1 cut(s) | 1524 |
| SfuI | TTCGAA | 1 cut(s) | 487 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1461 |
| SpeI | ACTAGT | 1 cut(s) | 1513 |
| Sse9I | AATT | 9 cut(s) | 167, 289, 936, 965, 1034, 1206, 1410, 1423, 1503 |
| SsiI | CCGC | 3 cut(s) | 482, 1042, 1232 |
| SspI | AATATT | 1 cut(s) | 743 |
| SspMI | CTAG | 3 cut(s) | 377, 753, 1514 |
| SstI | GAGCTC | 1 cut(s) | 837 |
| StyD4I | CCNGG | 1 cut(s) | 534 |
| StyI | CCWWGG | 1 cut(s) | 500 |
| TaaI | ACNGT | 3 cut(s) | 281, 724, 1283 |
| TaiI | ACGT | 3 cut(s) | 660, 927, 1202 |
| TaqI | TCGA | 5 cut(s) | 411, 487, 726, 795, 1023 |
| TasI | AATT | 9 cut(s) | 167, 289, 936, 965, 1034, 1206, 1410, 1423, 1503 |
| TatI | WGTACW | 2 cut(s) | 160, 1449 |
| TauI | GCSGC | 1 cut(s) | 484 |
| TfiI | GAWTC | 3 cut(s) | 125, 413, 548 |
| Tru1I | TTAA | 3 cut(s) | 431, 852, 1172 |
| Tru9I | TTAA | 3 cut(s) | 431, 852, 1172 |
| TscAI | CASTG | 1 cut(s) | 1068 |
| TseFI | GTSAC | 2 cut(s) | 104, 1090 |
| TseI | GCWGC | 1 cut(s) | 1101 |
| Tsp45I | GTSAC | 2 cut(s) | 104, 1090 |
| TspGWI | ACGGA | 1 cut(s) | 416 |
| TspRI | CASTG | 1 cut(s) | 1068 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 99 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 751 |
| XapI | RAATTY | 2 cut(s) | 289, 936 |
| XceI | RCATGY | 3 cut(s) | 161, 1057, 1078 |
| XmiI | GTMKAC | 3 cut(s) | 531, 702, 726 |
| XspI | CTAG | 3 cut(s) | 377, 753, 1514 |
| Zsp2I | ATGCAT | 1 cut(s) | 1354 |