FvH4_6g06540

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000053
Physical Location & Seq
Forward (+)
3834623 .. 3839563
4941 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g06540.t1

Sequence Viewer

Length: 1713 bp
ATGTCAATGATTACAGAACCTTCCCTATCAAGTCCAGTTCCACAGATTGCTATGCCTGAAATTGTTCGCCCCATAGCCAATTTTCAACCAAGCATTTGGGGAGATCAGTTCCTCAACTATGATTCTCAAGACATTAAGACTGGGGCCTTTTGGCAACAACAGGTCGAGGAGCTGAAAGTAAACGTGAAGAGTAAAGTGTTTACAAATGAATGTGATTTTGCGCTTCGACTCAAGTTGATCGATGCAATCCAACGCCTTGGAGTGGCATACCATTTTGAAGAAGAGATAGCAGATGCATTGCGACACATCCATGCTACATATCGTGATCAGGATCACTTCAATGATGATAGCGATCTTTCCACTGTTGCATTATGTTTTCGTTTGCTAAGGCAACATGGATATGAAATCTCATCCGATATGTTCAACAAGTTCAAAGACGAAAATGGTAGCTTCAAAGAAGGGTTAGTTGCCGATGGGTGTGCTATGCTGAGCCTCTACGAAGCTGCACATCTTAAGGTCCATGGAGAAAACATACTAGAAGAGGCTCTTGTTTTCACCACTGCTCACCTCGAGTCAGCAAAATCCAGTGCATGTTATTCAACACAAATGACTGAACAAATAACTCAAGCCTTGGTGAGACCTCTGCGAACAAGTCTAGAGAGGATATGCGCCAAGCGTCACATGTCAGTCTATCAACTCGAAGATGACGCCGCACTCATCAAACACAACGAAACATTACTGAAACTTGCAAAGATGGATTTCAATCTTGTTCAGTTCTTACACAAGAAGGAGTTGTGTGAGATTACTAGGTGGTGGAAAGAACTAGACTTTGAAAGGAAATTACCTTTTGCAAGAGACAGGAAGGTGGAGTTGTTCTTTTGGATAGTGGGAGTATATTTTGAACCACAGTACTCTATTGGCAGGATCTTTATGACCAAAGTGGCTATCCTGCTAACTGTTATGGATGATATCTATGATGCATATGGTACATTTGAAGAACTCGTCGTCTTCACTGAAGCAATTGACAGGTGGGATGTCAAATACATAGATGAACTGCCGGACTATTTGAAAATATTCTATTACGAACTTTTGAATGTTTTTCATGAAATGGACACAGTGTTGGCGAAAGAAGGAAGATCTTACCGAGTTTGCTACGCAATACAAGCTATGAAAGATCAAGCCCGATCGTATTTCAACGAAGCTCGGTGGTTGCACGAAGGACGCACTCCAAGCATGGAAGAATATATGAGTGTTGCAACGGTTTCTATTAGTTACACTTTTCTAACAACCATTTCATTACTTGGCATGGGGGATATTGTTACAAAGGATTCATTTGAGTGGTTGTCGAATGGCCCGAAAATTGTTCGAGCTTCTAATATCATTTTTAGGCTCATGGATGACATTGTTTCCACCAAGTTTGAGAAAGAGAGAGGAGGGCATGCACCTTCTAGCATTGATTGCTATACGAAGCAATATGGTGTTTCAGAACAAGAGGCGATTGATGTGTTCAACAAACAAATTGTGGAATCATGGAAGGATATAAACGAGGAATTTCTTAAACCCACTGCTGTGCCGATGCCGGTTCTTATGCGCGTTCTCAACTTAACAAGAGTAGCAGATCTCCTCTACAAAGGAGAAGATGGGTTCACACGGGTTGGGAAGATGACTAAAGATAGTGTTGCTGCAGTTATTATCGATTCAGTGCCACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

571

Amino Acids

65.79

Weight (kDa)

5.18

Isoelectric Point (pI)

42.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 32 - 211 1.5e-56 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 273 - 513 1.1e-95 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 313 - 513 2.6e-56 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1593
AciI CCGC 1 cut(s) 711
AclWI GGATC 2 cut(s) 339, 932
AcsI RAATTY 1 cut(s) 1550
AcuI CTGAAG 1 cut(s) 1035
AcyI GRCGYC 1 cut(s) 708
AfaI GTAC 2 cut(s) 911, 988
AfiI CCNNNNNNNGG 1 cut(s) 262
AflII CTTAAG 1 cut(s) 512
AflIII ACRYGT 1 cut(s) 681
AleI CACNNNNGTG 1 cut(s) 1568
AluBI AGCT 6 cut(s) 172, 450, 503, 1166, 1202, 1370
AluI AGCT 6 cut(s) 172, 450, 503, 1166, 1202, 1370
Alw26I GTCTC 2 cut(s) 631, 849
AlwI GGATC 2 cut(s) 339, 932
Ama87I CYCGRG 1 cut(s) 569
AoxI GGCC 2 cut(s) 144, 1351
ApeKI GCWGC 2 cut(s) 503, 1682
ApoI RAATTY 1 cut(s) 1550
Asp700I GAANNNNTTC 2 cut(s) 63, 1073
AspLEI GCGC 3 cut(s) 223, 671, 1593
AspS9I GGNCC 3 cut(s) 144, 517, 1352
AsuHPI GGTGA 3 cut(s) 547, 557, 646
AvaI CYCGRG 1 cut(s) 569
AvaII GGWCC 1 cut(s) 517
BbsI GAAGAC 1 cut(s) 1000
BbvI GCAGC 2 cut(s) 490, 1669
BccI CCATC 3 cut(s) 467, 748, 1634
BcgI CGANNNNNNTGC 2 cut(s) 461, 495
BclI TGATCA 1 cut(s) 325
BcoDI GTCTC 2 cut(s) 631, 849
BfaI CTAG 5 cut(s) 536, 656, 807, 824, 1449
BfmI CTRYAG 1 cut(s) 1683
BfrI CTTAAG 1 cut(s) 512
BglII AGATCT 2 cut(s) 1136, 1618
BisI GCNGC 3 cut(s) 504, 711, 1683
BlpI GCTNAGC 1 cut(s) 488
BlsI GCNGC 3 cut(s) 505, 712, 1684
BmcAI AGTACT 1 cut(s) 911
Bme18I GGWCC 1 cut(s) 517
BmeT110I CYCGRG 1 cut(s) 569
BmgT120I GGNCC 3 cut(s) 144, 517, 1352
BmiI GGNNCC 1 cut(s) 145
BmrI ACTGGG 1 cut(s) 150
BmsI GCATC 4 cut(s) 232, 283, 967, 1566
BmuI ACTGGG 1 cut(s) 150
BpiI GAAGAC 1 cut(s) 1000
Bpu10I CCTNAGC 1 cut(s) 386
Bpu1102I GCTNAGC 1 cut(s) 488
BpuEI CTTGAG 3 cut(s) 111, 215, 609
Bsa29I ATCGAT 2 cut(s) 240, 1695
BsaBI GATNNNNATC 3 cut(s) 330, 351, 762
BsaHI GRCGYC 1 cut(s) 708
BsaI GGTCTC 1 cut(s) 631
BsaJI CCNNGG 3 cut(s) 256, 520, 630
Bsc4I CCNNNNNNNGG 1 cut(s) 262
Bse118I RCCGGY 1 cut(s) 1579
Bse1I ACTGG 3 cut(s) 35, 145, 585
Bse3DI GCAATG 1 cut(s) 296
Bse8I GATNNNNATC 3 cut(s) 330, 351, 762
BseCI ATCGAT 2 cut(s) 240, 1695
BseDI CCNNGG 3 cut(s) 256, 520, 630
BseGI GGATG 5 cut(s) 306, 410, 970, 1039, 1402
BseJI GATNNNNATC 3 cut(s) 330, 351, 762
BseLI CCNNNNNNNGG 1 cut(s) 262
BseMI GCAATG 1 cut(s) 296
BseMII CTCAG 1 cut(s) 479
BseNI ACTGG 3 cut(s) 35, 145, 585
BseRI GAGGAG 3 cut(s) 182, 1446, 1613
BseXI GCAGC 2 cut(s) 490, 1669
BsgI GTGCAG 1 cut(s) 489
Bsh1236I CGCG 1 cut(s) 1593
Bsh1285I CGRYCG 1 cut(s) 1187
BshFI GGCC 2 cut(s) 146, 1353
BshVI ATCGAT 2 cut(s) 240, 1695
BsiEI CGRYCG 1 cut(s) 1187
BsiHKCI CYCGRG 1 cut(s) 569
BsiSI CCGG 2 cut(s) 1058, 1580
BslI CCNNNNNNNGG 1 cut(s) 262
BsmAI GTCTC 2 cut(s) 631, 849
BsnI GGCC 2 cut(s) 146, 1353
Bso31I GGTCTC 1 cut(s) 631
BsoBI CYCGRG 1 cut(s) 569
Bsp1720I GCTNAGC 1 cut(s) 488
Bsp19I CCATGG 1 cut(s) 520
BspACI CCGC 1 cut(s) 711
BspANI GGCC 2 cut(s) 146, 1353
BspCNI CTCAG 1 cut(s) 480
BspDI ATCGAT 2 cut(s) 240, 1695
BspFNI CGCG 1 cut(s) 1593
BspHI TCATGA 1 cut(s) 1102
BspLI GGNNCC 1 cut(s) 145
BspMAI CTGCAG 1 cut(s) 1687
BspPI GGATC 2 cut(s) 339, 932
BspTI CTTAAG 1 cut(s) 512
BspTNI GGTCTC 1 cut(s) 631
BsrDI GCAATG 1 cut(s) 296
BsrFI RCCGGY 1 cut(s) 1579
BsrI ACTGG 3 cut(s) 35, 145, 585
BssAI RCCGGY 1 cut(s) 1579
BssECI CCNNGG 3 cut(s) 256, 520, 630
BssNI GRCGYC 1 cut(s) 708
BssT1I CCWWGG 3 cut(s) 256, 520, 630
Bst4CI ACNGT 6 cut(s) 364, 909, 958, 1117, 1261, 1710
Bst6I CTCTTC 3 cut(s) 182, 276, 534
BstACI GRCGYC 1 cut(s) 708
BstAFI CTTAAG 1 cut(s) 512
BstAPI GCANNNNNTGC 1 cut(s) 1458
BstC8I GCNNGC 1 cut(s) 1440
BstDEI CTNAG 2 cut(s) 386, 488
BstDSI CCRYGG 1 cut(s) 520
BstF5I GGATG 5 cut(s) 306, 410, 970, 1039, 1402
BstFNI CGCG 1 cut(s) 1593
BstHHI GCGC 3 cut(s) 223, 671, 1593
BstMAI GTCTC 2 cut(s) 631, 849
BstMCI CGRYCG 1 cut(s) 1187
BstMWI GCNNNNNNNGC 3 cut(s) 1163, 1230, 1458
BstNSI RCATGY 3 cut(s) 594, 685, 1442
BstSFI CTRYAG 1 cut(s) 1683
BstUI CGCG 1 cut(s) 1593
BstV1I GCAGC 2 cut(s) 490, 1669
BstV2I GAAGAC 1 cut(s) 1000
BstX2I RGATCY 3 cut(s) 924, 1136, 1618
BstXI CCANNNNNNTGG 2 cut(s) 96, 257
BstYI RGATCY 3 cut(s) 924, 1136, 1618
Bsu15I ATCGAT 2 cut(s) 240, 1695
BsuRI GGCC 2 cut(s) 146, 1353
BsuTUI ATCGAT 2 cut(s) 240, 1695
BtgI CCRYGG 1 cut(s) 520
BtsCI GGATG 5 cut(s) 306, 410, 970, 1039, 1402
BtsI GCAGTG 2 cut(s) 558, 1563
BtsIMutI CAGTG 8 cut(s) 360, 558, 592, 1011, 1122, 1563, 1706, 1707
Cac8I GCNNGC 1 cut(s) 1440
CciI TCATGA 1 cut(s) 1102
CfoI GCGC 3 cut(s) 223, 671, 1593
Cfr10I RCCGGY 1 cut(s) 1579
Cfr13I GGNCC 3 cut(s) 144, 517, 1352
ClaI ATCGAT 2 cut(s) 240, 1695
CseI GACGC 3 cut(s) 665, 716, 1230
Csp6I GTAC 2 cut(s) 910, 987
CspCI CAANNNNNGTGG 2 cut(s) 1399, 1434
CviQI GTAC 2 cut(s) 910, 987
DdeI CTNAG 2 cut(s) 386, 488
Eam1104I CTCTTC 3 cut(s) 182, 276, 534
EarI CTCTTC 3 cut(s) 182, 276, 534
Eco130I CCWWGG 3 cut(s) 256, 520, 630
Eco31I GGTCTC 1 cut(s) 631
Eco32I GATATC 1 cut(s) 970
Eco47I GGWCC 1 cut(s) 517
Eco57I CTGAAG 1 cut(s) 1035
Eco88I CYCGRG 1 cut(s) 569
EcoO109I RGGNCCY 1 cut(s) 144
EcoRV GATATC 1 cut(s) 970
EcoT14I CCWWGG 3 cut(s) 256, 520, 630
EcoT22I ATGCAT 2 cut(s) 298, 982
ErhI CCWWGG 3 cut(s) 256, 520, 630
FalI AAGNNNNNCTT 2 cut(s) 531, 563
FauNDI CATATG 1 cut(s) 982
FbaI TGATCA 1 cut(s) 325
Fnu4HI GCNGC 3 cut(s) 504, 711, 1683
FokI GGATG 5 cut(s) 293, 397, 977, 1046, 1409
Fsp4HI GCNGC 3 cut(s) 504, 711, 1683
FspBI CTAG 5 cut(s) 536, 656, 807, 824, 1449
GlaI GCGC 3 cut(s) 222, 670, 1592
GluI GCNGC 3 cut(s) 504, 711, 1683
HaeIII GGCC 2 cut(s) 146, 1353
HapII CCGG 2 cut(s) 1058, 1580
HgaI GACGC 3 cut(s) 665, 716, 1230
HhaI GCGC 3 cut(s) 223, 671, 1593
Hin1I GRCGYC 1 cut(s) 708
Hin6I GCGC 3 cut(s) 221, 669, 1591
HinP1I GCGC 3 cut(s) 221, 669, 1591
HinfI GANTC 6 cut(s) 122, 228, 572, 1328, 1526, 1697
HpaII CCGG 2 cut(s) 1058, 1580
HphI GGTGA 3 cut(s) 547, 557, 646
Hpy166II GTNNAC 3 cut(s) 181, 201, 1647
Hpy188I TCNGA 2 cut(s) 415, 1486
Hpy188III TCNNGA 5 cut(s) 128, 323, 329, 656, 1103
Hpy8I GTNNAC 3 cut(s) 181, 201, 1647
Hpy99I CGWCG 1 cut(s) 1007
HpyAV CCTTC 8 cut(s) 30, 452, 781, 856, 1124, 1211, 1455, 1528
HpyCH4III ACNGT 6 cut(s) 364, 909, 958, 1117, 1261, 1710
HpyCH4IV ACGT 1 cut(s) 183
HpyF10VI GCNNNNNNNGC 3 cut(s) 1163, 1230, 1458
HpyF3I CTNAG 2 cut(s) 386, 488
HpySE526I ACGT 1 cut(s) 183
Hsp92I GRCGYC 1 cut(s) 708
HspAI GCGC 3 cut(s) 221, 669, 1591
Ksp22I TGATCA 1 cut(s) 325
LmnI GCTCC 1 cut(s) 169
Lsp1109I GCAGC 2 cut(s) 490, 1669
LweI GCATC 4 cut(s) 232, 283, 967, 1566
MaeI CTAG 5 cut(s) 536, 656, 807, 824, 1449
MaeII ACGT 1 cut(s) 183
MaeIII GTNAC 3 cut(s) 677, 1271, 1318
MfeI CAATTG 1 cut(s) 1020
MflI RGATCY 3 cut(s) 924, 1136, 1618
MluCI AATT 7 cut(s) 60, 79, 839, 1020, 1359, 1518, 1550
MlyI GAGTC 2 cut(s) 222, 581
MmeI TCCRAC 1 cut(s) 274
Mph1103I ATGCAT 2 cut(s) 298, 982
MroXI GAANNNNTTC 2 cut(s) 63, 1073
MseI TTAA 4 cut(s) 135, 513, 1557, 1604
MslI CAYNNNNRTG 5 cut(s) 309, 339, 399, 1336, 1568
MspCI CTTAAG 1 cut(s) 512
MspI CCGG 2 cut(s) 1058, 1580
MunI CAATTG 1 cut(s) 1020
MvnI CGCG 1 cut(s) 1593
MwoI GCNNNNNNNGC 3 cut(s) 1163, 1230, 1458
NcoI CCATGG 1 cut(s) 520
NdeI CATATG 1 cut(s) 982
NlaIV GGNNCC 1 cut(s) 145
NmuCI GTSAC 1 cut(s) 677
NsiI ATGCAT 2 cut(s) 298, 982
NspI RCATGY 3 cut(s) 594, 685, 1442
OliI CACNNNNGTG 1 cut(s) 1568
PaeI GCATGC 1 cut(s) 1442
PaeR7I CTCGAG 1 cut(s) 569
PagI TCATGA 1 cut(s) 1102
PciI ACATGT 1 cut(s) 681
PcsI WCGNNNNNNNCGW 1 cut(s) 1352
PdmI GAANNNNTTC 2 cut(s) 63, 1073
PfeI GAWTC 4 cut(s) 122, 1328, 1526, 1697
PkrI GCNGC 3 cut(s) 505, 712, 1684
Ple19I CGATCG 1 cut(s) 1187
PleI GAGTC 2 cut(s) 222, 580
PpsI GAGTC 2 cut(s) 222, 580
PscI ACATGT 1 cut(s) 681
PspN4I GGNNCC 1 cut(s) 145
PspPI GGNCC 3 cut(s) 144, 517, 1352
PspXI VCTCGAGB 1 cut(s) 569
PstI CTGCAG 1 cut(s) 1687
PsuI RGATCY 3 cut(s) 924, 1136, 1618
PvuI CGATCG 1 cut(s) 1187
RsaI GTAC 2 cut(s) 911, 988
RsaNI GTAC 2 cut(s) 910, 987
RseI CAYNNNNRTG 5 cut(s) 309, 339, 399, 1336, 1568
SaqAI TTAA 4 cut(s) 135, 513, 1557, 1604
SatI GCNGC 3 cut(s) 504, 711, 1683
Sau96I GGNCC 3 cut(s) 144, 517, 1352
ScaI AGTACT 1 cut(s) 911
SchI GAGTC 2 cut(s) 222, 581
SfaNI GCATC 4 cut(s) 232, 283, 967, 1566
SfcI CTRYAG 1 cut(s) 1683
Sfr274I CTCGAG 1 cut(s) 569
SinI GGWCC 1 cut(s) 517
SlaI CTCGAG 1 cut(s) 569
SmiMI CAYNNNNRTG 5 cut(s) 309, 339, 399, 1336, 1568
SmlI CTYRAG 5 cut(s) 126, 230, 512, 569, 624
SmoI CTYRAG 5 cut(s) 126, 230, 512, 569, 624
SphI GCATGC 1 cut(s) 1442
Sse9I AATT 7 cut(s) 60, 79, 839, 1020, 1359, 1518, 1550
SsiI CCGC 1 cut(s) 711
SspI AATATT 1 cut(s) 1074
SspMI CTAG 5 cut(s) 536, 656, 807, 824, 1449
StyI CCWWGG 3 cut(s) 256, 520, 630
TaaI ACNGT 6 cut(s) 364, 909, 958, 1117, 1261, 1710
TaiI ACGT 1 cut(s) 186
TaqI TCGA 8 cut(s) 165, 226, 240, 570, 699, 1346, 1366, 1695
TasI AATT 7 cut(s) 60, 79, 839, 1020, 1359, 1518, 1550
TatI WGTACW 1 cut(s) 909
TauI GCSGC 1 cut(s) 713
TfiI GAWTC 4 cut(s) 122, 1328, 1526, 1697
Tru1I TTAA 4 cut(s) 135, 513, 1557, 1604
Tru9I TTAA 4 cut(s) 135, 513, 1557, 1604
TscAI CASTG 8 cut(s) 367, 565, 592, 1018, 1122, 1570, 1707, 1713
TseFI GTSAC 1 cut(s) 677
TseI GCWGC 2 cut(s) 503, 1682
Tsp45I GTSAC 1 cut(s) 677
TspDTI ATGAA 8 cut(s) 222, 417, 1065, 1091, 1119, 1184, 1284, 1320
TspRI CASTG 8 cut(s) 367, 565, 592, 1018, 1122, 1570, 1707, 1713
Vha464I CTTAAG 1 cut(s) 512
VpaK11BI GGWCC 1 cut(s) 517
XapI RAATTY 1 cut(s) 1550
XbaI TCTAGA 1 cut(s) 655
XceI RCATGY 3 cut(s) 594, 685, 1442
XhoI CTCGAG 1 cut(s) 569
XmnI GAANNNNTTC 2 cut(s) 63, 1073
XspI CTAG 5 cut(s) 536, 656, 807, 824, 1449
ZrmI AGTACT 1 cut(s) 911
Zsp2I ATGCAT 2 cut(s) 298, 982
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.