MD11G1232100.v1.1

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000053
Physical Location & Seq
Reverse (-)
33831363 .. 33833855
2493 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1232100.v1.1.491

Sequence Viewer

Length: 1683 bp
ATGGCATTAATCCAGGATTCAGCAGAAGCTCAATCACAAAATGCTATGCCTGACGTTGCTCGGCGGACGGCAAATTTTCACCCAAGCGTTTGGGGAGATCAGTTCATAAATTATGATGATTCCCAAGACACTATTACTCAAGCTCAAAGTCAACAACAGGTTGATGAACTCAAAGAAATTGTGAGGGAAGCATTCACAACTAGTGCAACTGATTTTTCACTTCAGCTAAAGTTGATTGATGCAATTCAGCGCCTTGGCTTGGCATACCATTTTGAAAGAGAAATCAGAGAAGCACTTGAATGTATGCATTCTACGTTTGATGATGACCATGGCAACGATGATGTTGATCTATACACTGTTGCTTTAGGTTTCCGGCTGCTAAGACAACATGGATATAATGTTTCCTGTGCCATATTCAACAATTTCAAAGATGAAAGTAGTAACTTCAAGGAAAGCTTGATTGATGACATGTCTGGTATGCTAAGCTTTTATGAAGCAACACATCTGAGGGTGCATGGAGAAGACATATTAGAAGAGGCTCTAGTATTCACCACCACTCACCTTGAGTCAGCAGCAACCCGTGTAAGCAATCCACTAGCTACACAAATAACTCAAGCCCTAGAGAGACCTCTGCGAAAAAGTCTAGAGAGATTATCTGCGCGACGTTACATTTCAATCTACCAAGAAGAAGCTTCACATAATGAATCTCTTCTGAAACTTGCAAAGTTAGACTTCAATCTCGTCCAGCCCTTGCACAAAAAGGAGCTCCAAGAGATCACTAGGTGGTGGAGAGCACTAGACTTTGAAAGGAAGCTGCCATTTGCAAGAGATAGGATGGTAGAGTTGTACTTTTGGATCGTCGGAGTGTATTTTGAACCCCAGTACTCTGTTGGGAGAAAAATTATGACAAAAGTGAGTGTCTTGCTGACAATATTGGATGATATCTACGACGCATTTGGTACATTTGAAGAGCTCGTAGTCTTTACTGAAGCGATTGACAGGTGGGATGTCAATTGCATTGATGAACTCCCGGAATATATGCAAACATTTTATCATGCACTTTTAAATCTCTACAATGATATTGAGGCAGAGATGGTGAAGGAAGGAAGATCATACCGAGTTCCATATGCAATACAAGCTATGAAAGATCAAGCTCGATCCTATTTTAATGAGGCCCAATGGTTGCACGAGGGACGAGTTCCAAGCATGGAGGAGTATATGCGTGTTGCAACAGTTTCGATTAGTTACACCTTTCTTACAACCATATCTTTACTTGGCATGGGAGATGTTGTGACAAAGGAAGCATTTGAGTGGTTGTTCACTGACCCCAAAATTGTTAGAGCTGCAAATACAATTTTTAGGCTCATGGATGACATTGTTTCCACAGACTTTGAGAAAGAGAGAGGGCATGCTGCTTCTAGTGTTGACTGCTACATGAAGCAATATGGGGTGTCAGAGCAAGAGACAGTTGATGTTTTTCAGAAGCAAATTATGGATTTGTGGAAGGATATAAACGAGGAGTTCCTTAGACCAACTTCTGTGCCAATGCCTGTTCTTATGCGCGTTCTCAATTTAACAAGAGTAACTGATCTTCTTTACAAAGGGGAAGATGGATTCACACTTGTGGGAAAGGTGACGAGAGATAGTGTTACTTCAGTCTGTATCGATCCAGTGCCACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

561

Amino Acids

64.78

Weight (kDa)

4.8

Isoelectric Point (pI)

41.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 30 - 207 2.4e-59 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 266 - 503 6.8e-99 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 304 - 503 1.1e-60 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 661, 1563
AciI CCGC 1 cut(s) 64
AclWI GGATC 3 cut(s) 863, 1152, 1661
AcsI RAATTY 1 cut(s) 73
AcuI CTGAAG 3 cut(s) 206, 1008, 1638
AdeI CACNNNGTG 1 cut(s) 783
AfaI GTAC 3 cut(s) 848, 884, 961
AfiI CCNNNNNNNGG 1 cut(s) 259
AflIII ACRYGT 1 cut(s) 468
AhlI ACTAGT 1 cut(s) 200
AjnI CCWGG 1 cut(s) 12
AleI CACNNNNGTG 1 cut(s) 1622
Alw21I GWGCWC 3 cut(s) 768, 796, 975
Alw26I GTCTC 2 cut(s) 619, 1457
AlwI GGATC 3 cut(s) 863, 1152, 1661
AoxI GGCC 1 cut(s) 1173
ApeKI GCWGC 5 cut(s) 376, 572, 814, 1343, 1412
ApoI RAATTY 1 cut(s) 73
AseI ATTAAT 1 cut(s) 8
Asp700I GAANNNNTTC 1 cut(s) 708
AspLEI GCGC 3 cut(s) 252, 661, 1563
AspS9I GGNCC 1 cut(s) 1174
AsuC2I CCSGG 1 cut(s) 1031
AsuHPI GGTGA 5 cut(s) 71, 541, 551, 1108, 1645
BanII GRGCYC 2 cut(s) 768, 975
BarI GAAGNNNNNNTAC 2 cut(s) 1575, 1607
BauI CACGAG 1 cut(s) 1187
BbsI GAAGAC 1 cut(s) 528
Bbv12I GWGCWC 3 cut(s) 768, 796, 975
BbvI GCAGC 5 cut(s) 363, 584, 801, 1330, 1399
BccI CCATC 3 cut(s) 829, 1087, 1604
BceAI ACGGC 1 cut(s) 84
BcgI CGANNNNNNTGC 2 cut(s) 1218, 1252
BciT130I CCWGG 1 cut(s) 14
BcnI CCSGG 1 cut(s) 1031
BcoDI GTCTC 2 cut(s) 619, 1457
BcuI ACTAGT 1 cut(s) 200
BfaI CTAG 8 cut(s) 201, 542, 596, 620, 644, 780, 797, 1419
BfoI RGCGCY 1 cut(s) 253
BisI GCNGC 5 cut(s) 377, 573, 815, 1344, 1413
BlpI GCTNAGC 1 cut(s) 482
BlsI GCNGC 5 cut(s) 378, 574, 816, 1345, 1414
BmcAI AGTACT 1 cut(s) 884
Bme1390I CCNGG 2 cut(s) 14, 1031
BmgT120I GGNCC 1 cut(s) 1174
BmrFI CCNGG 2 cut(s) 14, 1031
BmrI ACTGGG 1 cut(s) 874
BmsI GCATC 1 cut(s) 229
BmuI ACTGGG 1 cut(s) 874
BpiI GAAGAC 1 cut(s) 528
Bpu1102I GCTNAGC 1 cut(s) 482
BpuEI CTTGAG 3 cut(s) 123, 584, 597
BpuMI CCSGG 1 cut(s) 1031
Bsa29I ATCGAT 1 cut(s) 1665
BsaBI GATNNNNATC 1 cut(s) 345
BsaI GGTCTC 1 cut(s) 619
BsaJI CCNNGG 2 cut(s) 253, 328
BsaXI ACNNNNNCTCC 2 cut(s) 1275, 1305
Bsc4I CCNNNNNNNGG 1 cut(s) 259
Bse1I ACTGG 2 cut(s) 880, 1670
Bse8I GATNNNNATC 1 cut(s) 345
BseBI CCWGG 1 cut(s) 14
BseCI ATCGAT 1 cut(s) 1665
BseDI CCNNGG 2 cut(s) 253, 328
BseGI GGATG 4 cut(s) 840, 943, 1012, 1375
BseJI GATNNNNATC 1 cut(s) 345
BseLI CCNNNNNNNGG 1 cut(s) 259
BseMII CTCAG 1 cut(s) 497
BseNI ACTGG 2 cut(s) 880, 1670
BseRI GAGGAG 2 cut(s) 1226, 1532
BseXI GCAGC 5 cut(s) 363, 584, 801, 1330, 1399
Bsh1236I CGCG 2 cut(s) 661, 1563
BshFI GGCC 1 cut(s) 1175
BshVI ATCGAT 1 cut(s) 1665
BsiHKAI GWGCWC 3 cut(s) 768, 796, 975
BsiSI CCGG 2 cut(s) 373, 1031
BslFI GGGAC 1 cut(s) 1206
BslI CCNNNNNNNGG 1 cut(s) 259
BsmAI GTCTC 2 cut(s) 619, 1457
BsmFI GGGAC 1 cut(s) 1206
BsmI GAATGC 2 cut(s) 191, 307
BsnI GGCC 1 cut(s) 1175
Bso31I GGTCTC 1 cut(s) 619
Bsp1286I GDGCHC 3 cut(s) 768, 796, 975
Bsp143I GATC 9 cut(s) 97, 346, 774, 855, 1109, 1147, 1157, 1588, 1666
Bsp1720I GCTNAGC 1 cut(s) 482
Bsp19I CCATGG 1 cut(s) 328
BspACI CCGC 1 cut(s) 64
BspANI GGCC 1 cut(s) 1175
BspCNI CTCAG 1 cut(s) 498
BspDI ATCGAT 1 cut(s) 1665
BspFNI CGCG 2 cut(s) 661, 1563
BspPI GGATC 3 cut(s) 863, 1152, 1661
BspQI GCTCTTC 1 cut(s) 963
BspTNI GGTCTC 1 cut(s) 619
BsrI ACTGG 2 cut(s) 880, 1670
BssECI CCNNGG 2 cut(s) 253, 328
BssMI GATC 9 cut(s) 97, 346, 774, 855, 1109, 1147, 1157, 1588, 1666
BssSI CACGAG 1 cut(s) 1187
BssT1I CCWWGG 2 cut(s) 253, 328
Bst2BI CACGAG 1 cut(s) 1187
Bst2UI CCWGG 1 cut(s) 14
Bst4CI ACNGT 3 cut(s) 358, 1234, 1468
Bst6I CTCTTC 3 cut(s) 528, 714, 963
BstC8I GCNNGC 1 cut(s) 1410
BstDEI CTNAG 4 cut(s) 380, 482, 506, 1526
BstDSI CCRYGG 1 cut(s) 328
BstF5I GGATG 4 cut(s) 840, 943, 1012, 1375
BstFNI CGCG 2 cut(s) 661, 1563
BstH2I RGCGCY 1 cut(s) 253
BstHHI GCGC 3 cut(s) 252, 661, 1563
BstKTI GATC 9 cut(s) 100, 349, 777, 858, 1112, 1150, 1160, 1591, 1669
BstMAI GTCTC 2 cut(s) 619, 1457
BstMBI GATC 9 cut(s) 97, 346, 774, 855, 1109, 1147, 1157, 1588, 1666
BstMWI GCNNNNNNNGC 1 cut(s) 1136
BstNI CCWGG 1 cut(s) 14
BstNSI RCATGY 2 cut(s) 472, 1412
BstSCI CCNGG 2 cut(s) 12, 1029
BstUI CGCG 2 cut(s) 661, 1563
BstV1I GCAGC 5 cut(s) 363, 584, 801, 1330, 1399
BstV2I GAAGAC 1 cut(s) 528
BstXI CCANNNNNNTGG 1 cut(s) 90
Bsu15I ATCGAT 1 cut(s) 1665
BsuRI GGCC 1 cut(s) 1175
BsuTUI ATCGAT 1 cut(s) 1665
BtgI CCRYGG 1 cut(s) 328
BtsCI GGATG 4 cut(s) 840, 943, 1012, 1375
BtsIMutI CAGTG 3 cut(s) 354, 1320, 1677
Cac8I GCNNGC 1 cut(s) 1410
CfoI GCGC 3 cut(s) 252, 661, 1563
Cfr13I GGNCC 1 cut(s) 1174
ClaI ATCGAT 1 cut(s) 1665
CseI GACGC 1 cut(s) 959
Csp6I GTAC 3 cut(s) 847, 883, 960
CspCI CAANNNNNGTGG 4 cut(s) 544, 579, 1372, 1407
CviQI GTAC 3 cut(s) 847, 883, 960
DdeI CTNAG 4 cut(s) 380, 482, 506, 1526
DpnI GATC 9 cut(s) 99, 348, 776, 857, 1111, 1149, 1159, 1590, 1668
DpnII GATC 9 cut(s) 97, 346, 774, 855, 1109, 1147, 1157, 1588, 1666
DraI TTTAAA 1 cut(s) 1065
DraIII CACNNNGTG 1 cut(s) 783
Eam1104I CTCTTC 3 cut(s) 528, 714, 963
EarI CTCTTC 3 cut(s) 528, 714, 963
EciI GGCGGA 1 cut(s) 79
Ecl136II GAGCTC 2 cut(s) 766, 973
Eco130I CCWWGG 2 cut(s) 253, 328
Eco24I GRGCYC 2 cut(s) 768, 975
Eco31I GGTCTC 1 cut(s) 619
Eco32I GATATC 1 cut(s) 943
Eco53kI GAGCTC 2 cut(s) 766, 973
Eco57I CTGAAG 3 cut(s) 206, 1008, 1638
EcoICRI GAGCTC 2 cut(s) 766, 973
EcoRII CCWGG 1 cut(s) 12
EcoRV GATATC 1 cut(s) 943
EcoT14I CCWWGG 2 cut(s) 253, 328
EcoT22I ATGCAT 1 cut(s) 309
EcoT38I GRGCYC 2 cut(s) 768, 975
ErhI CCWWGG 2 cut(s) 253, 328
FalI AAGNNNNNCTT 4 cut(s) 440, 472, 716, 748
FaqI GGGAC 1 cut(s) 1206
FauNDI CATATG 1 cut(s) 1126
Fnu4HI GCNGC 5 cut(s) 377, 573, 815, 1344, 1413
FokI GGATG 4 cut(s) 847, 950, 1019, 1382
FriOI GRGCYC 2 cut(s) 768, 975
Fsp4HI GCNGC 5 cut(s) 377, 573, 815, 1344, 1413
FspBI CTAG 8 cut(s) 201, 542, 596, 620, 644, 780, 797, 1419
GlaI GCGC 3 cut(s) 251, 660, 1562
GluI GCNGC 5 cut(s) 377, 573, 815, 1344, 1413
HaeII RGCGCY 1 cut(s) 253
HaeIII GGCC 1 cut(s) 1175
HapII CCGG 2 cut(s) 373, 1031
HgaI GACGC 1 cut(s) 959
HhaI GCGC 3 cut(s) 252, 661, 1563
Hin6I GCGC 3 cut(s) 250, 659, 1561
HinP1I GCGC 3 cut(s) 250, 659, 1561
HincII GTYRAC 2 cut(s) 152, 1426
HindII GTYRAC 2 cut(s) 152, 1426
HindIII AAGCTT 3 cut(s) 454, 484, 690
HinfI GANTC 5 cut(s) 17, 119, 566, 704, 1614
HpaII CCGG 2 cut(s) 373, 1031
HphI GGTGA 5 cut(s) 71, 541, 551, 1108, 1645
Hpy166II GTNNAC 3 cut(s) 152, 1320, 1426
Hpy188I TCNGA 6 cut(s) 287, 507, 714, 863, 1456, 1482
Hpy188III TCNNGA 1 cut(s) 644
Hpy8I GTNNAC 3 cut(s) 152, 1320, 1426
Hpy99I CGWCG 3 cut(s) 666, 863, 953
HpyAV CCTTC 3 cut(s) 1093, 1097, 1498
HpyCH4III ACNGT 3 cut(s) 358, 1234, 1468
HpyCH4IV ACGT 3 cut(s) 54, 314, 664
HpyF10VI GCNNNNNNNGC 1 cut(s) 1136
HpyF3I CTNAG 4 cut(s) 380, 482, 506, 1526
HpySE526I ACGT 3 cut(s) 54, 314, 664
HspAI GCGC 3 cut(s) 250, 659, 1561
Kzo9I GATC 9 cut(s) 97, 346, 774, 855, 1109, 1147, 1157, 1588, 1666
LguI GCTCTTC 1 cut(s) 963
LmnI GCTCC 2 cut(s) 763, 771
Lsp1109I GCAGC 5 cut(s) 363, 584, 801, 1330, 1399
LweI GCATC 1 cut(s) 229
MaeI CTAG 8 cut(s) 201, 542, 596, 620, 644, 780, 797, 1419
MaeII ACGT 3 cut(s) 54, 314, 664
MaeIII GTNAC 7 cut(s) 440, 665, 1244, 1291, 1582, 1633, 1648
MalI GATC 9 cut(s) 99, 348, 776, 857, 1111, 1149, 1159, 1590, 1668
MboI GATC 9 cut(s) 97, 346, 774, 855, 1109, 1147, 1157, 1588, 1666
MboII GAAGA 8 cut(s) 533, 545, 698, 701, 980, 1119, 1583, 1619
MfeI CAATTG 1 cut(s) 1012
MhlI GDGCHC 3 cut(s) 768, 796, 975
MlyI GAGTC 1 cut(s) 575
MmeI TCCRAC 1 cut(s) 841
Mph1103I ATGCAT 1 cut(s) 309
MroXI GAANNNNTTC 1 cut(s) 708
MseI TTAA 4 cut(s) 8, 1064, 1167, 1574
MslI CAYNNNNRTG 3 cut(s) 298, 1309, 1622
MspI CCGG 2 cut(s) 373, 1031
MspR9I CCNGG 2 cut(s) 14, 1031
MunI CAATTG 1 cut(s) 1012
Mva1269I GAATGC 2 cut(s) 191, 307
MvaI CCWGG 1 cut(s) 14
MvnI CGCG 2 cut(s) 661, 1563
MwoI GCNNNNNNNGC 1 cut(s) 1136
NciI CCSGG 1 cut(s) 1031
NcoI CCATGG 1 cut(s) 328
NdeI CATATG 1 cut(s) 1126
NdeII GATC 9 cut(s) 97, 346, 774, 855, 1109, 1147, 1157, 1588, 1666
NmeAIII GCCGAG 1 cut(s) 40
NmuCI GTSAC 2 cut(s) 1291, 1633
NsiI ATGCAT 1 cut(s) 309
NspI RCATGY 2 cut(s) 472, 1412
OliI CACNNNNGTG 1 cut(s) 1622
PaeI GCATGC 1 cut(s) 1412
PciI ACATGT 1 cut(s) 468
PciSI GCTCTTC 1 cut(s) 963
PctI GAATGC 2 cut(s) 191, 307
PdmI GAANNNNTTC 1 cut(s) 708
PfeI GAWTC 4 cut(s) 17, 119, 704, 1614
PfoI TCCNGGA 2 cut(s) 12, 1029
PkrI GCNGC 5 cut(s) 378, 574, 816, 1345, 1414
PleI GAGTC 1 cut(s) 574
PpsI GAGTC 1 cut(s) 574
PscI ACATGT 1 cut(s) 468
PshBI ATTAAT 1 cut(s) 8
Psp124BI GAGCTC 2 cut(s) 768, 975
Psp6I CCWGG 1 cut(s) 12
PspGI CCWGG 1 cut(s) 12
PspPI GGNCC 1 cut(s) 1174
RsaI GTAC 3 cut(s) 848, 884, 961
RsaNI GTAC 3 cut(s) 847, 883, 960
RseI CAYNNNNRTG 3 cut(s) 298, 1309, 1622
SacI GAGCTC 2 cut(s) 768, 975
SapI GCTCTTC 1 cut(s) 963
SaqAI TTAA 4 cut(s) 8, 1064, 1167, 1574
SatI GCNGC 5 cut(s) 377, 573, 815, 1344, 1413
Sau3AI GATC 9 cut(s) 97, 346, 774, 855, 1109, 1147, 1157, 1588, 1666
Sau96I GGNCC 1 cut(s) 1174
ScaI AGTACT 1 cut(s) 884
SchI GAGTC 1 cut(s) 575
ScrFI CCNGG 2 cut(s) 14, 1031
SduI GDGCHC 3 cut(s) 768, 796, 975
SfaNI GCATC 1 cut(s) 229
SmiMI CAYNNNNRTG 3 cut(s) 298, 1309, 1622
SmlI CTYRAG 3 cut(s) 138, 563, 612
SmoI CTYRAG 3 cut(s) 138, 563, 612
SpeI ACTAGT 1 cut(s) 200
SphI GCATGC 1 cut(s) 1412
SsiI CCGC 1 cut(s) 64
SspI AATATT 1 cut(s) 933
SspMI CTAG 8 cut(s) 201, 542, 596, 620, 644, 780, 797, 1419
SstI GAGCTC 2 cut(s) 768, 975
StyD4I CCNGG 2 cut(s) 12, 1029
StyI CCWWGG 2 cut(s) 253, 328
TaaI ACNGT 3 cut(s) 358, 1234, 1468
TaiI ACGT 3 cut(s) 57, 317, 667
TaqI TCGA 3 cut(s) 1156, 1238, 1665
TatI WGTACW 2 cut(s) 846, 882
TfiI GAWTC 4 cut(s) 17, 119, 704, 1614
Tru1I TTAA 4 cut(s) 8, 1064, 1167, 1574
Tru9I TTAA 4 cut(s) 8, 1064, 1167, 1574
TscAI CASTG 3 cut(s) 361, 1327, 1677
TseFI GTSAC 2 cut(s) 1291, 1633
TseI GCWGC 5 cut(s) 376, 572, 814, 1343, 1412
Tsp45I GTSAC 2 cut(s) 1291, 1633
TspDTI ATGAA 8 cut(s) 94, 180, 447, 507, 717, 1038, 1157, 1451
TspRI CASTG 3 cut(s) 361, 1327, 1677
VspI ATTAAT 1 cut(s) 8
XapI RAATTY 1 cut(s) 73
XbaI TCTAGA 1 cut(s) 643
XceI RCATGY 2 cut(s) 472, 1412
XcmI CCANNNNNNNNNTGG 1 cut(s) 887
XmnI GAANNNNTTC 1 cut(s) 708
XspI CTAG 8 cut(s) 201, 542, 596, 620, 644, 780, 797, 1419
ZrmI AGTACT 1 cut(s) 884
Zsp2I ATGCAT 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.