RLG00000023920

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000053
Physical Location & Seq
Reverse (-)
30051083 .. 30053469
2387 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023920

Sequence Viewer

Length: 1656 bp
ATGCCTGTCCAAACTATTCCAGCAGCTGCATCCCAGATCAATGCTAAGCCAGAAGTTGTTCGGCGCACAGCAAATTTTAAACCTAGCGTTTGGGGAGATCGGTTTCCTAACTATGCTGAAGACATTGTAACTCAATCTCATATGCAAAAACAAGTTGAGGAGCTGAAACAAGTAGTGAGGAAGGAAGTATTCACTGAGGCTGTTGATGATTTTTCACATCAACTGAAGCTAATTGATGCAATCCAGCGCCCTGGTGTGGCTTACCATTTTGAAGCTGAAATAGAACAAGCCCTGGAACGTATACATGCTACCTATCAAGATATTAATGATGATGATGGTGATCTGTACAATGTTGCTCTTCGTTTTCGACTACTCAGACGACATGGATATAATGTTTCCTGCGATGTATTCAACAAATTCAGAGATGTTAATGGTGACTTCAAGGAAAGCTTAGTTACTGACGTTTCTGGCATGCTAAGCTTCTATAAGGCAGCCCATCTAATGGTGCATGGAGAAAAATTACTAGAAGAGGCTCTCGTTTTTACCACCACTCATCTCGAGACTGCAACAACTTTAACCGGTGCAAGCTCTCTTCTGAAAGCAGAAATAACTGAAGCCCTAGAGAGACCTCTACTAAAAACTGTAGAGAGGTTAGGTGCTCGGCGTTACATGTCAATATACCAAGATGAAGCTTCACACAATAAAGACTTACTGAAACTTGCCAAAGTTAGATTTCAATGTTGTTCAGTGTTTACACAAAAAGGAACTCAATGGTATAAGGAACTAGACTTTGCAAGGAAGATGCCTTTTGCTCGAGATAGGCTCGTGGAATTGTTCTTTTGGATTATGGGAATATATTTTGAACCCCAATACGTGTTCGGGAGAAACATTCTGACGAAGCTGATTGAGATAGTAACAGTAATGGATGATATGTATGAAGCATTCGGTACATTTGAAGAACTCGTCATCTTAACTGAAACAATTGAGAGGTGGGATGCTAGTTGCATGGATCAACTGCCAGACTATATGCAACCATTTTATCTAACACTTCTGGATGTTGTCAATGAAGTTGGGGAGGAACTGATAAAGCAAGGAAGATCATACCGACTTTACTATGTGAAAGAAATTATGAAGAATCAAGCTCGGCTCTACTTTGCTGAGGCCAGATGGTTCCACGAAGGATGCACCCCAAGAGTGGATGAGTATATGCGTGTTGCGGCAACTTCTGTCGGTAACACTATGCTTTCAGTGGTATCTTTAGTAGGCATGGGAGACATTATAACAAAAGAGACATTTGAGTGGTTGACCAATGATCCTAAAATTCTTAGAGCTTCGAATACCATATTTAGGCTTATGGATGACATTGCTGGGCACAAGTTTGAGAAAGAGAGAGGGCATGTTGCTTCTAGTATCGATTGCTACATGAATGAATATGGGGTCTCAGAGCAAGAGACAATTGATGTCTTTAACAAACGAATTGTGGATTCGTGGAAGGATATAAACGAAGAGTTTCTGAGACCCACTGCTGCGCCAGTGCCTGTGCTCAATCGTGTTCTTAACCCAACTCGAGTGGTTGATCTCCTTTACAAAAAGGGGAGATGCCTTCACGCATGTTGGAAAACTGATGAAAGACACTATTGCTTCACTGTTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

552

Amino Acids

64.02

Weight (kDa)

5.59

Isoelectric Point (pI)

44.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 31 - 207 1.2e-55 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 260 - 499 1.2e-94 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 300 - 499 8e-56 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1280
AccB7I CCANNNNNTGG 1 cut(s) 502
AccI GTMKAC 1 cut(s) 301
AciI CCGC 1 cut(s) 1217
AclWI GGATC 2 cut(s) 1017, 1307
AcsI RAATTY 3 cut(s) 73, 416, 1320
AcuI CTGAAG 3 cut(s) 138, 245, 633
AfaI GTAC 2 cut(s) 347, 949
AfiI CCNNNNNNNGG 4 cut(s) 256, 502, 1195, 1347
AflIII ACRYGT 2 cut(s) 669, 873
AgeI ACCGGT 1 cut(s) 578
AgsI TTSAA 6 cut(s) 272, 412, 442, 737, 863, 956
AjnI CCWGG 2 cut(s) 250, 291
Alw21I GWGCWC 2 cut(s) 661, 1545
Alw26I GTCTC 7 cut(s) 554, 619, 1266, 1283, 1444, 1445, 1510
AlwI GGATC 2 cut(s) 1017, 1307
AlwNI CAGNNNCTG 2 cut(s) 26, 1538
Ama87I CYCGRG 3 cut(s) 557, 813, 1566
AoxI GGCC 1 cut(s) 1161
ApeKI GCWGC 4 cut(s) 23, 26, 491, 1526
ApoI RAATTY 3 cut(s) 73, 416, 1320
AseI ATTAAT 1 cut(s) 324
AsiGI ACCGGT 1 cut(s) 578
Asp700I GAANNNNTTC 2 cut(s) 57, 1509
AspLEI GCGC 3 cut(s) 66, 249, 1531
AsuHPI GGTGA 2 cut(s) 350, 446
AsuII TTCGAA 1 cut(s) 1334
AvaI CYCGRG 3 cut(s) 557, 813, 1566
BaeGI GKGCMC 1 cut(s) 1374
BauI CACGAG 1 cut(s) 824
BbsI GAAGAC 1 cut(s) 126
Bbv12I GWGCWC 2 cut(s) 661, 1545
BbvCI CCTCAGC 1 cut(s) 1158
BbvI GCAGC 4 cut(s) 13, 35, 503, 1513
BccI CCATC 3 cut(s) 329, 504, 1161
BciT130I CCWGG 2 cut(s) 252, 293
BcoDI GTCTC 7 cut(s) 554, 619, 1266, 1283, 1444, 1445, 1510
BfaI CTAG 6 cut(s) 84, 524, 620, 785, 999, 1407
BfmI CTRYAG 1 cut(s) 642
BfoI RGCGCY 1 cut(s) 250
BisI GCNGC 5 cut(s) 24, 27, 492, 1218, 1527
BlpI GCTNAGC 2 cut(s) 45, 476
BlsI GCNGC 5 cut(s) 25, 28, 493, 1219, 1528
Bme1390I CCNGG 2 cut(s) 252, 293
BmeT110I CYCGRG 3 cut(s) 557, 813, 1566
BmiI GGNNCC 1 cut(s) 1172
BmrFI CCNGG 2 cut(s) 252, 293
BmsI GCATC 6 cut(s) 38, 226, 792, 985, 1172, 1589
BpiI GAAGAC 1 cut(s) 126
BplI GAGNNNNNCTC 2 cut(s) 807, 839
Bpu10I CCTNAGC 1 cut(s) 1158
Bpu1102I GCTNAGC 2 cut(s) 45, 476
Bpu14I TTCGAA 1 cut(s) 1334
Bsa29I ATCGAT 1 cut(s) 1413
BsaAI YACGTR 1 cut(s) 874
BsaBI GATNNNNATC 1 cut(s) 339
BsaI GGTCTC 3 cut(s) 619, 1444, 1510
BsaJI CCNNGG 2 cut(s) 250, 291
BsaWI WCCGGW 1 cut(s) 578
Bsc4I CCNNNNNNNGG 4 cut(s) 256, 502, 1195, 1347
Bse118I RCCGGY 1 cut(s) 578
Bse1I ACTGG 1 cut(s) 1532
Bse3DI GCAATG 1 cut(s) 1362
Bse8I GATNNNNATC 1 cut(s) 339
BseBI CCWGG 2 cut(s) 252, 293
BseCI ATCGAT 1 cut(s) 1413
BseDI CCNNGG 2 cut(s) 250, 291
BseGI GGATG 7 cut(s) 29, 931, 1000, 1060, 1187, 1204, 1363
BseJI GATNNNNATC 1 cut(s) 339
BseLI CCNNNNNNNGG 4 cut(s) 256, 502, 1195, 1347
BseMI GCAATG 1 cut(s) 1362
BseMII CTCAG 5 cut(s) 186, 388, 1149, 1455, 1505
BseNI ACTGG 1 cut(s) 1532
BseRI GAGGAG 1 cut(s) 173
BseSI GKGCMC 1 cut(s) 1374
BseXI GCAGC 4 cut(s) 13, 35, 503, 1513
BseYI CCCAGC 1 cut(s) 1367
BshFI GGCC 1 cut(s) 1163
BshTI ACCGGT 1 cut(s) 578
BshVI ATCGAT 1 cut(s) 1413
BsiHKAI GWGCWC 2 cut(s) 661, 1545
BsiHKCI CYCGRG 3 cut(s) 557, 813, 1566
BsiSI CCGG 1 cut(s) 579
BslI CCNNNNNNNGG 4 cut(s) 256, 502, 1195, 1347
BsmAI GTCTC 7 cut(s) 554, 619, 1266, 1283, 1444, 1445, 1510
BsmI GAATGC 1 cut(s) 941
BsnI GGCC 1 cut(s) 1163
Bso31I GGTCTC 3 cut(s) 619, 1444, 1510
BsoBI CYCGRG 3 cut(s) 557, 813, 1566
Bsp119I TTCGAA 1 cut(s) 1334
Bsp1286I GDGCHC 3 cut(s) 661, 1374, 1545
Bsp1407I TGTACA 1 cut(s) 345
Bsp143I GATC 7 cut(s) 36, 97, 340, 1009, 1097, 1312, 1576
Bsp1720I GCTNAGC 2 cut(s) 45, 476
BspACI CCGC 1 cut(s) 1217
BspANI GGCC 1 cut(s) 1163
BspCNI CTCAG 5 cut(s) 187, 387, 1150, 1454, 1506
BspDI ATCGAT 1 cut(s) 1413
BspLI GGNNCC 1 cut(s) 1172
BspPI GGATC 2 cut(s) 1017, 1307
BspQI GCTCTTC 1 cut(s) 363
BspT104I TTCGAA 1 cut(s) 1334
BspTNI GGTCTC 3 cut(s) 619, 1444, 1510
BsrDI GCAATG 1 cut(s) 1362
BsrFI RCCGGY 1 cut(s) 578
BsrGI TGTACA 1 cut(s) 345
BsrI ACTGG 1 cut(s) 1532
BssAI RCCGGY 1 cut(s) 578
BssECI CCNNGG 2 cut(s) 250, 291
BssMI GATC 7 cut(s) 36, 97, 340, 1009, 1097, 1312, 1576
BssNAI GTATAC 1 cut(s) 302
BssSI CACGAG 1 cut(s) 824
Bst1107I GTATAC 1 cut(s) 302
Bst2BI CACGAG 1 cut(s) 824
Bst2UI CCWGG 2 cut(s) 252, 293
Bst4CI ACNGT 3 cut(s) 643, 919, 1648
Bst6I CTCTTC 4 cut(s) 363, 522, 597, 1500
BstAUI TGTACA 1 cut(s) 345
BstBAI YACGTR 1 cut(s) 874
BstBI TTCGAA 1 cut(s) 1334
BstC8I GCNNGC 2 cut(s) 473, 586
BstDEI CTNAG 9 cut(s) 45, 195, 374, 451, 476, 1158, 1325, 1441, 1514
BstF5I GGATG 7 cut(s) 29, 931, 1000, 1060, 1187, 1204, 1363
BstH2I RGCGCY 1 cut(s) 250
BstHHI GCGC 3 cut(s) 66, 249, 1531
BstKTI GATC 7 cut(s) 39, 100, 343, 1012, 1100, 1315, 1579
BstMAI GTCTC 7 cut(s) 554, 619, 1266, 1283, 1444, 1445, 1510
BstMBI GATC 7 cut(s) 36, 97, 340, 1009, 1097, 1312, 1576
BstMWI GCNNNNNNNGC 1 cut(s) 477
BstNI CCWGG 2 cut(s) 252, 293
BstNSI RCATGY 5 cut(s) 308, 475, 673, 1400, 1614
BstSCI CCNGG 2 cut(s) 250, 291
BstSFI CTRYAG 1 cut(s) 642
BstSLI GKGCMC 1 cut(s) 1374
BstV1I GCAGC 4 cut(s) 13, 35, 503, 1513
BstV2I GAAGAC 1 cut(s) 126
BstXI CCANNNNNNTGG 1 cut(s) 251
BstZ17I GTATAC 1 cut(s) 302
Bsu15I ATCGAT 1 cut(s) 1413
BsuRI GGCC 1 cut(s) 1163
BsuTUI ATCGAT 1 cut(s) 1413
BtgZI GCGATG 1 cut(s) 417
BtsCI GGATG 7 cut(s) 29, 931, 1000, 1060, 1187, 1204, 1363
BtsI GCAGTG 1 cut(s) 1521
BtsIMutI CAGTG 6 cut(s) 192, 753, 1254, 1521, 1539, 1644
Cac8I GCNNGC 2 cut(s) 473, 586
CaiI CAGNNNCTG 2 cut(s) 26, 1538
CfoI GCGC 3 cut(s) 66, 249, 1531
Cfr10I RCCGGY 1 cut(s) 578
ClaI ATCGAT 1 cut(s) 1413
Csp6I GTAC 2 cut(s) 346, 948
CspAI ACCGGT 1 cut(s) 578
CspCI CAANNNNNGTGG 2 cut(s) 1551, 1586
CviQI GTAC 2 cut(s) 346, 948
DdeI CTNAG 9 cut(s) 45, 195, 374, 451, 476, 1158, 1325, 1441, 1514
DpnI GATC 7 cut(s) 38, 99, 342, 1011, 1099, 1314, 1578
DpnII GATC 7 cut(s) 36, 97, 340, 1009, 1097, 1312, 1576
DraI TTTAAA 1 cut(s) 79
Eam1104I CTCTTC 4 cut(s) 363, 522, 597, 1500
EarI CTCTTC 4 cut(s) 363, 522, 597, 1500
Eco31I GGTCTC 3 cut(s) 619, 1444, 1510
Eco57I CTGAAG 3 cut(s) 138, 245, 633
Eco88I CYCGRG 3 cut(s) 557, 813, 1566
EcoRII CCWGG 2 cut(s) 250, 291
FalI AAGNNNNNCTT 2 cut(s) 434, 466
FauNDI CATATG 1 cut(s) 141
FblI GTMKAC 1 cut(s) 301
Fnu4HI GCNGC 5 cut(s) 24, 27, 492, 1218, 1527
FokI GGATG 7 cut(s) 16, 938, 1007, 1067, 1194, 1211, 1370
Fsp4HI GCNGC 5 cut(s) 24, 27, 492, 1218, 1527
FspBI CTAG 6 cut(s) 84, 524, 620, 785, 999, 1407
GlaI GCGC 3 cut(s) 65, 248, 1530
GluI GCNGC 5 cut(s) 24, 27, 492, 1218, 1527
GsaI CCCAGC 1 cut(s) 1371
HaeII RGCGCY 1 cut(s) 250
HaeIII GGCC 1 cut(s) 1163
HapII CCGG 1 cut(s) 579
HhaI GCGC 3 cut(s) 66, 249, 1531
Hin6I GCGC 3 cut(s) 64, 247, 1529
HinP1I GCGC 3 cut(s) 64, 247, 1529
HincII GTYRAC 1 cut(s) 1305
HindII GTYRAC 1 cut(s) 1305
HindIII AAGCTT 3 cut(s) 448, 478, 690
HinfI GANTC 2 cut(s) 1135, 1484
HpaII CCGG 1 cut(s) 579
HphI GGTGA 2 cut(s) 350, 446
Hpy166II GTNNAC 3 cut(s) 302, 753, 1305
Hpy188I TCNGA 6 cut(s) 377, 422, 597, 894, 1444, 1515
Hpy188III TCNNGA 6 cut(s) 317, 557, 559, 815, 880, 1052
Hpy8I GTNNAC 3 cut(s) 302, 753, 1305
HpyAV CCTTC 4 cut(s) 175, 1172, 1486, 1613
HpyCH4III ACNGT 3 cut(s) 643, 919, 1648
HpyCH4IV ACGT 3 cut(s) 298, 462, 873
HpyF10VI GCNNNNNNNGC 1 cut(s) 477
HpyF3I CTNAG 9 cut(s) 45, 195, 374, 451, 476, 1158, 1325, 1441, 1514
HpySE526I ACGT 3 cut(s) 298, 462, 873
HspAI GCGC 3 cut(s) 64, 247, 1529
Kzo9I GATC 7 cut(s) 36, 97, 340, 1009, 1097, 1312, 1576
LguI GCTCTTC 1 cut(s) 363
LmnI GCTCC 1 cut(s) 160
Lsp1109I GCAGC 4 cut(s) 13, 35, 503, 1513
LweI GCATC 6 cut(s) 38, 226, 792, 985, 1172, 1589
MaeI CTAG 6 cut(s) 84, 524, 620, 785, 999, 1407
MaeII ACGT 3 cut(s) 298, 462, 873
MaeIII GTNAC 6 cut(s) 127, 434, 454, 665, 913, 1232
MalI GATC 7 cut(s) 38, 99, 342, 1011, 1099, 1314, 1578
MboI GATC 7 cut(s) 36, 97, 340, 1009, 1097, 1312, 1576
MboII GAAGA 9 cut(s) 131, 350, 539, 584, 811, 968, 1107, 1144, 1517
MfeI CAATTG 2 cut(s) 981, 1455
MhlI GDGCHC 3 cut(s) 661, 1374, 1545
MmeI TCCRAC 1 cut(s) 1595
MroXI GAANNNNTTC 2 cut(s) 57, 1509
MseI TTAA 7 cut(s) 78, 324, 429, 575, 971, 1467, 1557
MslI CAYNNNNRTG 1 cut(s) 1297
MspA1I CMGCKG 1 cut(s) 26
MspI CCGG 1 cut(s) 579
MspR9I CCNGG 2 cut(s) 252, 293
MunI CAATTG 2 cut(s) 981, 1455
Mva1269I GAATGC 1 cut(s) 941
MvaI CCWGG 2 cut(s) 252, 293
MwoI GCNNNNNNNGC 1 cut(s) 477
NdeI CATATG 1 cut(s) 141
NdeII GATC 7 cut(s) 36, 97, 340, 1009, 1097, 1312, 1576
NlaIV GGNNCC 1 cut(s) 1172
NmeAIII GCCGAG 2 cut(s) 640, 1123
NmuCI GTSAC 1 cut(s) 434
NspI RCATGY 5 cut(s) 308, 475, 673, 1400, 1614
NspV TTCGAA 1 cut(s) 1334
PaeI GCATGC 1 cut(s) 475
PaeR7I CTCGAG 3 cut(s) 557, 813, 1566
PciI ACATGT 1 cut(s) 669
PciSI GCTCTTC 1 cut(s) 363
PctI GAATGC 1 cut(s) 941
PdmI GAANNNNTTC 2 cut(s) 57, 1509
PfeI GAWTC 2 cut(s) 1135, 1484
PflMI CCANNNNNTGG 1 cut(s) 502
PinAI ACCGGT 1 cut(s) 578
PkrI GCNGC 5 cut(s) 25, 28, 493, 1219, 1528
Ppu21I YACGTR 1 cut(s) 874
PscI ACATGT 1 cut(s) 669
PshBI ATTAAT 1 cut(s) 324
PsiI TTATAA 1 cut(s) 1280
Psp6I CCWGG 2 cut(s) 250, 291
PspFI CCCAGC 1 cut(s) 1367
PspGI CCWGG 2 cut(s) 250, 291
PspN4I GGNNCC 1 cut(s) 1172
PspXI VCTCGAGB 1 cut(s) 1566
PstNI CAGNNNCTG 2 cut(s) 26, 1538
PvuII CAGCTG 1 cut(s) 26
RsaI GTAC 2 cut(s) 347, 949
RsaNI GTAC 2 cut(s) 346, 948
RseI CAYNNNNRTG 1 cut(s) 1297
SapI GCTCTTC 1 cut(s) 363
SaqAI TTAA 7 cut(s) 78, 324, 429, 575, 971, 1467, 1557
SatI GCNGC 5 cut(s) 24, 27, 492, 1218, 1527
Sau3AI GATC 7 cut(s) 36, 97, 340, 1009, 1097, 1312, 1576
ScrFI CCNGG 2 cut(s) 252, 293
SduI GDGCHC 3 cut(s) 661, 1374, 1545
SfaNI GCATC 6 cut(s) 38, 226, 792, 985, 1172, 1589
SfcI CTRYAG 1 cut(s) 642
Sfr274I CTCGAG 3 cut(s) 557, 813, 1566
SfuI TTCGAA 1 cut(s) 1334
SlaI CTCGAG 3 cut(s) 557, 813, 1566
SmiMI CAYNNNNRTG 1 cut(s) 1297
SmlI CTYRAG 3 cut(s) 557, 813, 1566
SmoI CTYRAG 3 cut(s) 557, 813, 1566
SphI GCATGC 1 cut(s) 475
SsiI CCGC 1 cut(s) 1217
SspMI CTAG 6 cut(s) 84, 524, 620, 785, 999, 1407
StyD4I CCNGG 2 cut(s) 250, 291
TaaI ACNGT 3 cut(s) 643, 919, 1648
TaiI ACGT 3 cut(s) 301, 465, 876
TaqI TCGA 6 cut(s) 367, 558, 814, 1334, 1413, 1567
TatI WGTACW 1 cut(s) 345
TauI GCSGC 1 cut(s) 1220
TfiI GAWTC 2 cut(s) 1135, 1484
Tru1I TTAA 7 cut(s) 78, 324, 429, 575, 971, 1467, 1557
Tru9I TTAA 7 cut(s) 78, 324, 429, 575, 971, 1467, 1557
TscAI CASTG 6 cut(s) 199, 753, 1254, 1528, 1539, 1651
TseFI GTSAC 1 cut(s) 434
TseI GCWGC 4 cut(s) 23, 26, 491, 1526
Tsp45I GTSAC 1 cut(s) 434
TspDTI ATGAA 7 cut(s) 702, 951, 1080, 1145, 1439, 1443, 1641
TspRI CASTG 6 cut(s) 199, 753, 1254, 1528, 1539, 1651
Van91I CCANNNNNTGG 1 cut(s) 502
VspI ATTAAT 1 cut(s) 324
XapI RAATTY 3 cut(s) 73, 416, 1320
XceI RCATGY 5 cut(s) 308, 475, 673, 1400, 1614
XhoI CTCGAG 3 cut(s) 557, 813, 1566
XmiI GTMKAC 1 cut(s) 301
XmnI GAANNNNTTC 2 cut(s) 57, 1509
XspI CTAG 6 cut(s) 84, 524, 620, 785, 999, 1407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.