Rroxscaffold_1G00016230

(-)-germacrene D synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000053
Physical Location & Seq
Forward (+)
20229698 .. 20232097
2400 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00016230.1

Sequence Viewer

Length: 1536 bp
ATGTCTCTCAACGGGGTCAATATAGCTTCTCAAGCCCCTCCAACACTGAATGCCTCTACTGATGATGCCGATAGTCGACGTTCAGCAAATTTCCATCCCAGCATTTGGGGAGACCAGTTTCTGTCTTGTAACAACACTATGGAAACTAACATAAATGGTGAGCAACATGTCCAACAACTGAAGGAAGAAGTGAAGAGAGTGCTAATGGCTCCGACAGTTGAAACTTGTTTGGGAAAACTGGAGTTGATTGATGACATTCAACGCTTGGGCGTGTCTTACCATTTTGAATACGAGATTGACCAAACTATGCAGCAAATTCACGACAATTTTAATGGTTACTGTGATGGTGATCTGCACAGTACTTGTGCTCTTCGTTTTCGCTTGTTGAGGCAACATGGTTACAATGTTTCATGCGATATGTTCAACAAGTTCGACTACACATTGCTTCACGATGATGTAGTGGGGCTACAAAGCTTGTATGAAGCCACACATCTTAGAGTACGAGGAGAGGATTTTCTAGAAGAAGCACTAGCCTTCACCACCGCACATCTAGAGTCGGCAGAGCTAGCAAACCGTTTAAGCCCCCGCTTTCGAAACAAGTGGTGGAAGGAGTTGGACTTTGCCAGCAAGCTACCTTTCGCAAGAGACAGAGTAATTGAGTGCTACTTCTGGATTTTGGGAGTCTACTTTGAGCCTGAGTATTACTTTGCCAGGAGGACACTAACCAAAGTTATTGCCATGACGTCCATTATCGATGATATTTATGATGTGTATGGCACGCTTGAAGAACTAGACCTCTTTACTAGAGCAATTGAGAGGTGGGACATCTCAGCCATGGATGAACTACCGGAGTATATGAAAGTCTGTTACGAGGCACTGTTAGATGTCTATGCTGAAACAGAACACGAGCTTGCAAGCAAGGGACGATTATATCGTATCAATTACGCAAAAGAAGCGATGAAGAGACAGGTTACGGCATACCATGCTGAAGCAAAATGGTTTCACAAGAACTACACACCAACAATGGATGAATACATGGAAGTAGCCCTTGTCACTTCAGCCTACTCAATGCTAGCAACCACATCCTTTGTTGGAATGGGAGATATCGTTACAAAAGACTCCTTTGAGTGGATCTTCAGTGACCCTAAGATTGTAAAGGCTTCAGCAGTAGTTTGCAGACTCATGGATGACATTGTATCCCACAAGTTTGAGCAAAAGAGAGGACATGTTGCCTCGGCTGTGGAATGTTACATGAAACAATACGGTGCGACAGAAGAAGAAACCATAATTGAATTTCGTAAACAAGTGAGTGATGCATGGAAGGACATAAATCAAGAGTGCCTCCACCCTACACCTCTTGACATGCCCCTCTTAATGCGAGTTCTGAATCTCACACGCGTGATAGATGTAGTATACAAGTCTGAAGATGGCTACACTCATGCTGGAACCATTCTCAAAGATTTTGTAGCTTTCTCTACTTATCGAACCTGTGCCTGTATAAGCGGAAAAACTGGGAACTTGTTTTATTTATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

511

Amino Acids

59.04

Weight (kDa)

5.12

Isoelectric Point (pI)

42.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 35 - 189 1.7e-46 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 203 - 442 1.7e-106 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 243 - 442 1.2e-63 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1534
AatII GACGTC 1 cut(s) 746
AccB7I CCANNNNNTGG 1 cut(s) 105
AccI GTMKAC 3 cut(s) 76, 684, 1413
AccII CGCG 1 cut(s) 1398
AciI CCGC 3 cut(s) 543, 586, 1503
AclWI GGATC 1 cut(s) 1139
AcsI RAATTY 3 cut(s) 88, 315, 1292
AcuI CTGAAG 6 cut(s) 200, 1008, 1041, 1120, 1146, 1443
AcyI GRCGYC 1 cut(s) 743
AfaI GTAC 2 cut(s) 361, 501
AfiI CCNNNNNNNGG 2 cut(s) 105, 1128
AflIII ACRYGT 3 cut(s) 166, 1225, 1396
AgsI TTSAA 6 cut(s) 221, 260, 287, 424, 785, 1292
AjnI CCWGG 1 cut(s) 710
AleI CACNNNNGTG 1 cut(s) 1397
AluBI AGCT 6 cut(s) 26, 474, 565, 631, 910, 1469
AluI AGCT 6 cut(s) 26, 474, 565, 631, 910, 1469
Alw21I GWGCWC 1 cut(s) 370
Alw26I GTCTC 4 cut(s) 9, 105, 639, 958
AlwI GGATC 1 cut(s) 1139
AlwNI CAGNNNCTG 1 cut(s) 121
ApeKI GCWGC 1 cut(s) 310
ApoI RAATTY 3 cut(s) 88, 315, 1292
AsuHPI GGTGA 3 cut(s) 170, 359, 529
AsuII TTCGAA 1 cut(s) 592
AsuNHI GCTAGC 2 cut(s) 565, 1072
BauI CACGAG 1 cut(s) 905
Bbv12I GWGCWC 1 cut(s) 370
BbvI GCAGC 1 cut(s) 322
BccI CCATC 3 cut(s) 102, 338, 1421
BceAI ACGGC 1 cut(s) 990
BciT130I CCWGG 1 cut(s) 712
BciVI GTATCC 1 cut(s) 1207
BcoDI GTCTC 4 cut(s) 9, 105, 639, 958
BfaI CTAG 7 cut(s) 518, 530, 551, 566, 791, 804, 1073
BfuI GTATCC 1 cut(s) 1207
BisI GCNGC 1 cut(s) 311
BlsI GCNGC 1 cut(s) 312
BmcAI AGTACT 1 cut(s) 361
Bme1390I CCNGG 1 cut(s) 712
BmiI GGNNCC 2 cut(s) 210, 1447
BmrFI CCNGG 1 cut(s) 712
BmrI ACTGGG 1 cut(s) 1521
BmsI GCATC 2 cut(s) 55, 1303
BmtI GCTAGC 2 cut(s) 569, 1076
BmuI ACTGGG 1 cut(s) 1521
BpmI CTGGAG 1 cut(s) 260
Bpu14I TTCGAA 1 cut(s) 592
BpuEI CTTGAG 1 cut(s) 15
Bsa29I ATCGAT 1 cut(s) 753
BsaBI GATNNNNATC 1 cut(s) 348
BsaHI GRCGYC 1 cut(s) 743
BsaI GGTCTC 1 cut(s) 105
BsaJI CCNNGG 2 cut(s) 834, 1233
BsaWI WCCGGW 1 cut(s) 847
BsaXI ACNNNNNCTCC 2 cut(s) 1092, 1122
Bsc4I CCNNNNNNNGG 2 cut(s) 105, 1128
Bse1I ACTGG 3 cut(s) 115, 243, 1516
Bse3DI GCAATG 1 cut(s) 440
Bse8I GATNNNNATC 1 cut(s) 348
BseBI CCWGG 1 cut(s) 712
BseCI ATCGAT 1 cut(s) 753
BseDI CCNNGG 2 cut(s) 834, 1233
BseGI GGATG 5 cut(s) 94, 844, 1033, 1082, 1192
BseJI GATNNNNATC 1 cut(s) 348
BseLI CCNNNNNNNGG 2 cut(s) 105, 1128
BseMI GCAATG 1 cut(s) 440
BseMII CTCAG 2 cut(s) 687, 843
BseNI ACTGG 3 cut(s) 115, 243, 1516
BseRI GAGGAG 1 cut(s) 519
BseXI GCAGC 1 cut(s) 322
BseYI CCCAGC 1 cut(s) 98
BsgI GTGCAG 1 cut(s) 338
Bsh1236I CGCG 1 cut(s) 1398
BshVI ATCGAT 1 cut(s) 753
BsiHKAI GWGCWC 1 cut(s) 370
BsiSI CCGG 1 cut(s) 848
BslFI GGGAC 2 cut(s) 836, 936
BslI CCNNNNNNNGG 2 cut(s) 105, 1128
BsmAI GTCTC 4 cut(s) 9, 105, 639, 958
BsmFI GGGAC 2 cut(s) 836, 936
BsmI GAATGC 1 cut(s) 55
Bso31I GGTCTC 1 cut(s) 105
Bsp119I TTCGAA 1 cut(s) 592
Bsp1286I GDGCHC 1 cut(s) 370
Bsp143I GATC 2 cut(s) 349, 1131
Bsp19I CCATGG 1 cut(s) 834
BspACI CCGC 3 cut(s) 543, 586, 1503
BspCNI CTCAG 2 cut(s) 688, 842
BspDI ATCGAT 1 cut(s) 753
BspFNI CGCG 1 cut(s) 1398
BspLI GGNNCC 2 cut(s) 210, 1447
BspOI GCTAGC 2 cut(s) 569, 1076
BspPI GGATC 1 cut(s) 1139
BspQI GCTCTTC 1 cut(s) 375
BspT104I TTCGAA 1 cut(s) 592
BspTNI GGTCTC 1 cut(s) 105
BsrDI GCAATG 1 cut(s) 440
BsrI ACTGG 3 cut(s) 115, 243, 1516
BssECI CCNNGG 2 cut(s) 834, 1233
BssMI GATC 2 cut(s) 349, 1131
BssNAI GTATAC 1 cut(s) 1414
BssNI GRCGYC 1 cut(s) 743
BssSI CACGAG 1 cut(s) 905
BssT1I CCWWGG 1 cut(s) 834
Bst1107I GTATAC 1 cut(s) 1414
Bst2BI CACGAG 1 cut(s) 905
Bst2UI CCWGG 1 cut(s) 712
Bst4CI ACNGT 6 cut(s) 217, 341, 359, 575, 879, 1265
Bst6I CTCTTC 3 cut(s) 188, 375, 956
BstACI GRCGYC 1 cut(s) 743
BstAPI GCANNNNNTGC 1 cut(s) 983
BstBI TTCGAA 1 cut(s) 592
BstC8I GCNNGC 7 cut(s) 567, 625, 629, 779, 912, 916, 1074
BstDEI CTNAG 4 cut(s) 494, 696, 829, 1146
BstDSI CCRYGG 1 cut(s) 834
BstF5I GGATG 5 cut(s) 94, 844, 1033, 1082, 1192
BstFNI CGCG 1 cut(s) 1398
BstKTI GATC 2 cut(s) 352, 1134
BstMAI GTCTC 4 cut(s) 9, 105, 639, 958
BstMBI GATC 2 cut(s) 349, 1131
BstMWI GCNNNNNNNGC 4 cut(s) 32, 566, 953, 983
BstNI CCWGG 1 cut(s) 712
BstNSI RCATGY 3 cut(s) 170, 1229, 1366
BstSCI CCNGG 1 cut(s) 710
BstUI CGCG 1 cut(s) 1398
BstV1I GCAGC 1 cut(s) 322
BstX2I RGATCY 1 cut(s) 1131
BstYI RGATCY 1 cut(s) 1131
BstZ17I GTATAC 1 cut(s) 1414
Bsu15I ATCGAT 1 cut(s) 753
BsuI GTATCC 1 cut(s) 1207
BsuTUI ATCGAT 1 cut(s) 753
BtgI CCRYGG 1 cut(s) 834
BtgZI GCGATG 1 cut(s) 971
BtsCI GGATG 5 cut(s) 94, 844, 1033, 1082, 1192
BtsIMutI CAGTG 3 cut(s) 44, 875, 1144
Cac8I GCNNGC 7 cut(s) 567, 625, 629, 779, 912, 916, 1074
CaiI CAGNNNCTG 1 cut(s) 121
ClaI ATCGAT 1 cut(s) 753
Csp6I GTAC 2 cut(s) 360, 500
CspCI CAANNNNNGTGG 2 cut(s) 1069, 1104
CviQI GTAC 2 cut(s) 360, 500
DdeI CTNAG 4 cut(s) 494, 696, 829, 1146
DpnI GATC 2 cut(s) 351, 1133
DpnII GATC 2 cut(s) 349, 1131
Eam1104I CTCTTC 3 cut(s) 188, 375, 956
EarI CTCTTC 3 cut(s) 188, 375, 956
Eco130I CCWWGG 1 cut(s) 834
Eco31I GGTCTC 1 cut(s) 105
Eco32I GATATC 1 cut(s) 1105
Eco57I CTGAAG 6 cut(s) 200, 1008, 1041, 1120, 1146, 1443
EcoRII CCWGG 1 cut(s) 710
EcoRV GATATC 1 cut(s) 1105
EcoT14I CCWWGG 1 cut(s) 834
EcoT22I ATGCAT 1 cut(s) 1318
ErhI CCWWGG 1 cut(s) 834
FalI AAGNNNNNCTT 2 cut(s) 1032, 1064
FaqI GGGAC 2 cut(s) 836, 936
FauI CCCGC 1 cut(s) 593
FblI GTMKAC 3 cut(s) 76, 684, 1413
Fnu4HI GCNGC 1 cut(s) 311
FokI GGATG 5 cut(s) 81, 851, 1040, 1069, 1199
Fsp4HI GCNGC 1 cut(s) 311
FspBI CTAG 7 cut(s) 518, 530, 551, 566, 791, 804, 1073
GluI GCNGC 1 cut(s) 311
GsaI CCCAGC 1 cut(s) 102
GsuI CTGGAG 1 cut(s) 260
HapII CCGG 1 cut(s) 848
Hin1I GRCGYC 1 cut(s) 743
HincII GTYRAC 1 cut(s) 77
HindII GTYRAC 1 cut(s) 77
HindIII AAGCTT 1 cut(s) 472
HinfI GANTC 5 cut(s) 554, 681, 1118, 1179, 1387
HpaII CCGG 1 cut(s) 848
HphI GGTGA 3 cut(s) 170, 359, 529
Hpy166II GTNNAC 4 cut(s) 77, 685, 1301, 1414
Hpy188I TCNGA 3 cut(s) 213, 1386, 1423
Hpy188III TCNNGA 7 cut(s) 320, 449, 518, 551, 670, 1334, 1358
Hpy8I GTNNAC 4 cut(s) 77, 685, 1301, 1414
Hpy99I CGWCG 1 cut(s) 81
HpyAV CCTTC 4 cut(s) 175, 544, 601, 1315
HpyCH4III ACNGT 6 cut(s) 217, 341, 359, 575, 879, 1265
HpyCH4IV ACGT 2 cut(s) 79, 743
HpyCH4V TGCA 5 cut(s) 310, 355, 914, 1176, 1316
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 566, 953, 983
HpyF3I CTNAG 4 cut(s) 494, 696, 829, 1146
HpySE526I ACGT 2 cut(s) 79, 743
Hsp92I GRCGYC 1 cut(s) 743
Kzo9I GATC 2 cut(s) 349, 1131
LguI GCTCTTC 1 cut(s) 375
LmnI GCTCC 1 cut(s) 214
Lsp1109I GCAGC 1 cut(s) 322
LweI GCATC 2 cut(s) 55, 1303
MaeI CTAG 7 cut(s) 518, 530, 551, 566, 791, 804, 1073
MaeII ACGT 2 cut(s) 79, 743
MaeIII GTNAC 9 cut(s) 128, 335, 398, 866, 970, 1051, 1108, 1139, 1247
MalI GATC 2 cut(s) 351, 1133
MboI GATC 2 cut(s) 349, 1131
MfeI CAATTG 1 cut(s) 810
MflI RGATCY 1 cut(s) 1131
MhlI GDGCHC 1 cut(s) 370
MluCI AATT 8 cut(s) 88, 315, 325, 654, 810, 940, 1287, 1292
MluI ACGCGT 1 cut(s) 1396
MlyI GAGTC 4 cut(s) 563, 690, 1112, 1173
MmeI TCCRAC 5 cut(s) 65, 196, 236, 594, 1072
Mph1103I ATGCAT 1 cut(s) 1318
MseI TTAA 3 cut(s) 330, 578, 1373
MslI CAYNNNNRTG 2 cut(s) 453, 1397
MspI CCGG 1 cut(s) 848
MspR9I CCNGG 1 cut(s) 712
MunI CAATTG 1 cut(s) 810
Mva1269I GAATGC 1 cut(s) 55
MvaI CCWGG 1 cut(s) 712
MvnI CGCG 1 cut(s) 1398
MwoI GCNNNNNNNGC 4 cut(s) 32, 566, 953, 983
NcoI CCATGG 1 cut(s) 834
NdeII GATC 2 cut(s) 349, 1131
NheI GCTAGC 2 cut(s) 565, 1072
NlaIV GGNNCC 2 cut(s) 210, 1447
NmeAIII GCCGAG 1 cut(s) 1214
NmuCI GTSAC 2 cut(s) 1051, 1139
NsiI ATGCAT 1 cut(s) 1318
NspI RCATGY 3 cut(s) 170, 1229, 1366
NspV TTCGAA 1 cut(s) 592
OliI CACNNNNGTG 1 cut(s) 1397
PciI ACATGT 2 cut(s) 166, 1225
PciSI GCTCTTC 1 cut(s) 375
PcsI WCGNNNNNNNCGW 1 cut(s) 931
PctI GAATGC 1 cut(s) 55
PfeI GAWTC 1 cut(s) 1387
PflMI CCANNNNNTGG 1 cut(s) 105
PkrI GCNGC 1 cut(s) 312
PleI GAGTC 4 cut(s) 562, 689, 1112, 1173
PpsI GAGTC 4 cut(s) 562, 689, 1112, 1173
PscI ACATGT 2 cut(s) 166, 1225
PsiI TTATAA 1 cut(s) 1534
Psp6I CCWGG 1 cut(s) 710
PspFI CCCAGC 1 cut(s) 98
PspGI CCWGG 1 cut(s) 710
PspN4I GGNNCC 2 cut(s) 210, 1447
PstNI CAGNNNCTG 1 cut(s) 121
PsuI RGATCY 1 cut(s) 1131
RsaI GTAC 2 cut(s) 361, 501
RsaNI GTAC 2 cut(s) 360, 500
RseI CAYNNNNRTG 2 cut(s) 453, 1397
SalI GTCGAC 1 cut(s) 75
SapI GCTCTTC 1 cut(s) 375
SaqAI TTAA 3 cut(s) 330, 578, 1373
SatI GCNGC 1 cut(s) 311
Sau3AI GATC 2 cut(s) 349, 1131
ScaI AGTACT 1 cut(s) 361
SchI GAGTC 4 cut(s) 563, 690, 1112, 1173
ScrFI CCNGG 1 cut(s) 712
SduI GDGCHC 1 cut(s) 370
SfaNI GCATC 2 cut(s) 55, 1303
SfuI TTCGAA 1 cut(s) 592
SmiMI CAYNNNNRTG 2 cut(s) 453, 1397
SmlI CTYRAG 1 cut(s) 30
SmoI CTYRAG 1 cut(s) 30
Sse9I AATT 8 cut(s) 88, 315, 325, 654, 810, 940, 1287, 1292
SsiI CCGC 3 cut(s) 543, 586, 1503
SspMI CTAG 7 cut(s) 518, 530, 551, 566, 791, 804, 1073
StyD4I CCNGG 1 cut(s) 710
StyI CCWWGG 1 cut(s) 834
TaaI ACNGT 6 cut(s) 217, 341, 359, 575, 879, 1265
TaiI ACGT 2 cut(s) 82, 746
TaqI TCGA 5 cut(s) 76, 432, 592, 753, 1483
TasI AATT 8 cut(s) 88, 315, 325, 654, 810, 940, 1287, 1292
TatI WGTACW 1 cut(s) 359
TfiI GAWTC 1 cut(s) 1387
Tru1I TTAA 3 cut(s) 330, 578, 1373
Tru9I TTAA 3 cut(s) 330, 578, 1373
TscAI CASTG 3 cut(s) 51, 882, 1144
TseFI GTSAC 2 cut(s) 1051, 1139
TseI GCWGC 1 cut(s) 310
Tsp45I GTSAC 2 cut(s) 1051, 1139
TspDTI ATGAA 7 cut(s) 399, 495, 855, 872, 974, 1044, 1268
TspRI CASTG 3 cut(s) 51, 882, 1144
Van91I CCANNNNNTGG 1 cut(s) 105
XapI RAATTY 3 cut(s) 88, 315, 1292
XbaI TCTAGA 2 cut(s) 517, 550
XceI RCATGY 3 cut(s) 170, 1229, 1366
XmiI GTMKAC 3 cut(s) 76, 684, 1413
XspI CTAG 7 cut(s) 518, 530, 551, 566, 791, 804, 1073
ZraI GACGTC 1 cut(s) 744
ZrmI AGTACT 1 cut(s) 361
Zsp2I ATGCAT 1 cut(s) 1318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.