Rh6DG124300

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000053
Physical Location & Seq
Forward (+)
17469972 .. 17472531
2560 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG124300.1

Sequence Viewer

Length: 1698 bp
ATGTCGTCTATGCCAACTAGTTCTGTATCATTTCCGGTACCACAAATTGCAAAGCCTGAAATCATTCGCCGGACAGCAAATTTTCAACCAAGCATTTTGGGAGATCAGTTCATCAACTATGATTCCCAAAAAACTAAGACTAATGCCCTTTGGCATCAGCAAGTTGAAAAATTGAAAACAGTAGTAAAGAGGGAACTCTTTACTAATGAAGGTGATTTTTCACATCGACTCAAGTTAATTGATGCAATCCAACAACTTGGCGTGGCATATCATTTTGAACACGAAATAGAAGAGTCACTGCAACATATCCATGCAACATATCGTGATCATGATCTGTACCGTGACGATGGCCATCTTTACGATGTTGCCCTATTGTTTCGGCTTCTAAGGCAACATGGATATCAAATTTCATCTGATATATTCAACAAGTTCAAAGATTCAAATGGTAGCTTCAAAGAATGCTTAATTTCTGACGCCTCTGGTATGCTAAGCCTTTATGAAGCAACACATCTTAGGGTTAATGGAGAAGATATACTTGAAGAGGCTCTTCTGTTCACCACCACTCAACTCAAGCACGTGCTCAAAAGCAATGTAAGCTACCCACCAGTACAGAAGGAACAAATAACTGAAGCTTTGACAAGACCTCTTCGAAAAACTCTAGAGAGATTATGCGCCAAGCGTTACATTTCAGTCTACCAAGATGAAGCTTCACACAATGAATCATTGCTGAAACTTGCAAAGTTGGATTTCAATCTTGTTCAGTCTTTACACCAAAATGAGCTCTCTGATATTACTAGGTGGTGGAAAGAACTAGACTTTGAAAGGAAATTGCCTTTTTCCAGAGACAGGAAGGTGGAGTTGTTCTTTTGGATAGTGGGAGTATATTTTGAACCCCAGTACTCTATTGGCAGAATCATTATGACAAAAGTGGCTATCCTCCTAACTGTTTTGGATGATATCTATGATGCATATGGTACATTTGAAGAACTTGTGATCTTCACTGAAGCAATTGACAGGTGGAATGTCAAATGCATAGATGGACTCCCAGACTATTTGAAAATATTCTATCATGAACTTTTGAATCTTTTCAATGAAATTGACAAAGTGATGTCAAAGGAAGGAAGATCATATCGAACTTGCTATGCAATACAAGCTATGAAAGTTCAAGCTCGATCGTATTTCAATGAGGCCCAATGGTTGCACGAAGGACACACTCCGAGCATGGAGGAGTATATGAGTGTTGCTACAGTTTCCATTACTTACACTTTTCTAACAACTATTTCATTACTTGGCATGGGAGATATTGTAACGAAGGATGCATTTGAGTGGTTGTTGGATGATCCGAAAATTGTTAGAGCTTCCAATATCATTTTTAGGCTCATGGATGACATTGTTTCCAGCAAGTTTGAGAAAGAGAGAGGGCATACACCTTCTAGCATTGATTGCTATATGAAGCAATATGGTGTTTCAGAGCAAGAGACGATTGAAGTTTTCGAGAAACAAATTGCAAAATCATGGAAGGATATAAATGAGGAGTTTCTTAAACCAACAGCTGTGCCAATGCCGGTTCTTATGCGTGTTCTCAATTTAATGAGAGTAGTGGATCTTCTTTACAAGGGAGAAGATGGATTCACACGGGTTGGGAAGATGACCAAAGATAGTGTTGCTGCAGTTATTATCGACTCAGTGCCACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

565

Amino Acids

65.63

Weight (kDa)

5.71

Isoelectric Point (pI)

43.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 34 - 212 6.5e-55 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 269 - 508 2.2e-97 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 309 - 508 4.6e-58 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 37
AccB1I GGYRCC 1 cut(s) 37
AccI GTMKAC 1 cut(s) 693
AclWI GGATC 2 cut(s) 1334, 1611
AcoI YGGCCR 1 cut(s) 349
AcsI RAATTY 2 cut(s) 79, 405
AcuI CTGAAG 2 cut(s) 648, 1023
AcvI CACGTG 1 cut(s) 577
AcyI GRCGYC 1 cut(s) 474
AfaI GTAC 5 cut(s) 39, 338, 609, 899, 976
AfiI CCNNNNNNNGG 1 cut(s) 846
AhlI ACTAGT 1 cut(s) 17
AluBI AGCT 9 cut(s) 450, 597, 632, 707, 781, 1154, 1169, 1358, 1553
AluI AGCT 9 cut(s) 450, 597, 632, 707, 781, 1154, 1169, 1358, 1553
Alw21I GWGCWC 2 cut(s) 582, 783
Alw26I GTCTC 2 cut(s) 837, 1472
AlwI GGATC 2 cut(s) 1334, 1611
AoxI GGCC 2 cut(s) 349, 1188
ApeKI GCWGC 1 cut(s) 1667
ApoI RAATTY 2 cut(s) 79, 405
Asp700I GAANNNNTTC 3 cut(s) 63, 1061, 1085
Asp718I GGTACC 1 cut(s) 37
AspLEI GCGC 1 cut(s) 674
AspS9I GGNCC 1 cut(s) 1189
AsuHPI GGTGA 2 cut(s) 224, 547
AsuII TTCGAA 1 cut(s) 649
BalI TGGCCA 1 cut(s) 351
BanI GGYRCC 1 cut(s) 37
BanII GRGCYC 1 cut(s) 783
BarI GAAGNNNNNNTAC 2 cut(s) 1590, 1622
BbrPI CACGTG 1 cut(s) 577
Bbv12I GWGCWC 2 cut(s) 582, 783
BbvI GCAGC 1 cut(s) 1654
BccI CCATC 4 cut(s) 341, 360, 1031, 1619
BclI TGATCA 1 cut(s) 325
BcoDI GTCTC 2 cut(s) 837, 1472
BcuI ACTAGT 1 cut(s) 17
BfaI CTAG 5 cut(s) 18, 659, 795, 812, 1434
BfmI CTRYAG 2 cut(s) 1245, 1668
BisI GCNGC 1 cut(s) 1668
BlpI GCTNAGC 1 cut(s) 488
BlsI GCNGC 1 cut(s) 1669
BmcAI AGTACT 1 cut(s) 899
BmgT120I GGNCC 1 cut(s) 1189
BmiI GGNNCC 1 cut(s) 39
BmrI ACTGGG 1 cut(s) 889
BmsI GCATC 4 cut(s) 163, 232, 955, 1306
BmuI ACTGGG 1 cut(s) 889
Bpu1102I GCTNAGC 1 cut(s) 488
Bpu14I TTCGAA 1 cut(s) 649
BpuEI CTTGAG 2 cut(s) 215, 554
BsaAI YACGTR 1 cut(s) 577
BsaBI GATNNNNATC 3 cut(s) 330, 351, 750
BsaHI GRCGYC 1 cut(s) 474
BsaWI WCCGGW 1 cut(s) 34
BsaXI ACNNNNNCTCC 2 cut(s) 1290, 1320
Bsc4I CCNNNNNNNGG 1 cut(s) 846
Bse118I RCCGGY 1 cut(s) 1564
Bse1I ACTGG 2 cut(s) 605, 895
Bse3DI GCAATG 2 cut(s) 595, 722
Bse8I GATNNNNATC 3 cut(s) 330, 351, 750
BseGI GGATG 4 cut(s) 958, 1321, 1342, 1390
BseJI GATNNNNATC 3 cut(s) 330, 351, 750
BseLI CCNNNNNNNGG 1 cut(s) 846
BseMI GCAATG 2 cut(s) 595, 722
BseMII CTCAG 1 cut(s) 1698
BseNI ACTGG 2 cut(s) 605, 895
BseRI GAGGAG 2 cut(s) 1241, 1547
BseXI GCAGC 1 cut(s) 1654
Bsh1285I CGRYCG 1 cut(s) 1175
BshFI GGCC 2 cut(s) 351, 1190
BshNI GGYRCC 1 cut(s) 37
BsiEI CGRYCG 1 cut(s) 1175
BsiHKAI GWGCWC 2 cut(s) 582, 783
BsiSI CCGG 3 cut(s) 35, 70, 1565
BslI CCNNNNNNNGG 1 cut(s) 846
BsmAI GTCTC 2 cut(s) 837, 1472
BsmBI CGTCTC 1 cut(s) 1472
BsmI GAATGC 1 cut(s) 464
BsnI GGCC 2 cut(s) 351, 1190
Bsp119I TTCGAA 1 cut(s) 649
Bsp1286I GDGCHC 2 cut(s) 582, 783
Bsp143I GATC 8 cut(s) 103, 325, 331, 993, 1124, 1172, 1339, 1603
Bsp1720I GCTNAGC 1 cut(s) 488
BspANI GGCC 2 cut(s) 351, 1190
BspCNI CTCAG 1 cut(s) 1697
BspHI TCATGA 2 cut(s) 328, 1069
BspLI GGNNCC 1 cut(s) 39
BspMAI CTGCAG 1 cut(s) 1672
BspPI GGATC 2 cut(s) 1334, 1611
BspQI GCTCTTC 1 cut(s) 552
BspT104I TTCGAA 1 cut(s) 649
BspT107I GGYRCC 1 cut(s) 37
BsrDI GCAATG 2 cut(s) 595, 722
BsrFI RCCGGY 1 cut(s) 1564
BsrI ACTGG 2 cut(s) 605, 895
BssAI RCCGGY 1 cut(s) 1564
BssMI GATC 8 cut(s) 103, 325, 331, 993, 1124, 1172, 1339, 1603
BssNI GRCGYC 1 cut(s) 474
Bst4CI ACNGT 5 cut(s) 181, 341, 946, 1249, 1695
Bst6I CTCTTC 4 cut(s) 285, 534, 552, 651
BstACI GRCGYC 1 cut(s) 474
BstAPI GCANNNNNTGC 1 cut(s) 1443
BstBAI YACGTR 1 cut(s) 577
BstBI TTCGAA 1 cut(s) 649
BstDEI CTNAG 5 cut(s) 135, 386, 488, 512, 1684
BstF5I GGATG 4 cut(s) 958, 1321, 1342, 1390
BstHHI GCGC 1 cut(s) 674
BstKTI GATC 8 cut(s) 106, 328, 334, 996, 1127, 1175, 1342, 1606
BstMAI GTCTC 2 cut(s) 837, 1472
BstMBI GATC 8 cut(s) 103, 325, 331, 993, 1124, 1172, 1339, 1603
BstMCI CGRYCG 1 cut(s) 1175
BstMWI GCNNNNNNNGC 4 cut(s) 388, 594, 1151, 1443
BstSFI CTRYAG 2 cut(s) 1245, 1668
BstV1I GCAGC 1 cut(s) 1654
BstX2I RGATCY 1 cut(s) 1603
BstXI CCANNNNNNTGG 1 cut(s) 257
BstYI RGATCY 1 cut(s) 1603
BsuRI GGCC 2 cut(s) 351, 1190
BtsCI GGATG 4 cut(s) 958, 1321, 1342, 1390
BtsI GCAGTG 1 cut(s) 296
BtsIMutI CAGTG 4 cut(s) 296, 999, 1691, 1692
CciI TCATGA 2 cut(s) 328, 1069
CfoI GCGC 1 cut(s) 674
Cfr10I RCCGGY 1 cut(s) 1564
Cfr13I GGNCC 1 cut(s) 1189
CseI GACGC 1 cut(s) 482
Csp6I GTAC 5 cut(s) 38, 337, 608, 898, 975
CviAII CATG 8 cut(s) 311, 329, 395, 1070, 1222, 1294, 1381, 1515
CviQI GTAC 5 cut(s) 38, 337, 608, 898, 975
DdeI CTNAG 5 cut(s) 135, 386, 488, 512, 1684
DpnI GATC 8 cut(s) 105, 327, 333, 995, 1126, 1174, 1341, 1605
DpnII GATC 8 cut(s) 103, 325, 331, 993, 1124, 1172, 1339, 1603
EaeI YGGCCR 1 cut(s) 349
Eam1104I CTCTTC 4 cut(s) 285, 534, 552, 651
EarI CTCTTC 4 cut(s) 285, 534, 552, 651
Ecl136II GAGCTC 1 cut(s) 781
Eco24I GRGCYC 1 cut(s) 783
Eco32I GATATC 2 cut(s) 401, 958
Eco53kI GAGCTC 1 cut(s) 781
Eco57I CTGAAG 2 cut(s) 648, 1023
Eco72I CACGTG 1 cut(s) 577
EcoICRI GAGCTC 1 cut(s) 781
EcoRV GATATC 2 cut(s) 401, 958
EcoT22I ATGCAT 3 cut(s) 970, 1034, 1321
EcoT38I GRGCYC 1 cut(s) 783
Esp3I CGTCTC 1 cut(s) 1472
FaeI CATG 8 cut(s) 314, 332, 398, 1073, 1225, 1297, 1384, 1518
FalI AAGNNNNNCTT 4 cut(s) 519, 551, 531, 563
FatI CATG 8 cut(s) 310, 328, 394, 1069, 1221, 1293, 1380, 1514
FauNDI CATATG 1 cut(s) 970
FbaI TGATCA 1 cut(s) 325
FblI GTMKAC 1 cut(s) 693
Fnu4HI GCNGC 1 cut(s) 1668
FokI GGATG 4 cut(s) 965, 1328, 1349, 1397
FriOI GRGCYC 1 cut(s) 783
Fsp4HI GCNGC 1 cut(s) 1668
FspBI CTAG 5 cut(s) 18, 659, 795, 812, 1434
GlaI GCGC 1 cut(s) 673
GluI GCNGC 1 cut(s) 1668
HaeIII GGCC 2 cut(s) 351, 1190
HapII CCGG 3 cut(s) 35, 70, 1565
HgaI GACGC 1 cut(s) 482
HhaI GCGC 1 cut(s) 674
Hin1I GRCGYC 1 cut(s) 474
Hin1II CATG 8 cut(s) 314, 332, 398, 1073, 1225, 1297, 1384, 1518
Hin6I GCGC 1 cut(s) 672
HinP1I GCGC 1 cut(s) 672
HindIII AAGCTT 2 cut(s) 630, 705
HpaII CCGG 3 cut(s) 35, 70, 1565
HphI GGTGA 2 cut(s) 224, 547
Hpy166II GTNNAC 2 cut(s) 555, 694
Hpy188I TCNGA 6 cut(s) 415, 472, 787, 1218, 1344, 1471
Hpy188III TCNNGA 6 cut(s) 323, 329, 659, 840, 1070, 1495
Hpy8I GTNNAC 2 cut(s) 555, 694
HpyAV CCTTC 8 cut(s) 203, 607, 844, 1112, 1199, 1306, 1440, 1513
HpyCH4III ACNGT 5 cut(s) 181, 341, 946, 1249, 1695
HpyCH4IV ACGT 1 cut(s) 576
HpyF10VI GCNNNNNNNGC 4 cut(s) 388, 594, 1151, 1443
HpyF3I CTNAG 5 cut(s) 135, 386, 488, 512, 1684
HpySE526I ACGT 1 cut(s) 576
Hsp92I GRCGYC 1 cut(s) 474
Hsp92II CATG 8 cut(s) 314, 332, 398, 1073, 1225, 1297, 1384, 1518
HspAI GCGC 1 cut(s) 672
KpnI GGTACC 1 cut(s) 41
Ksp22I TGATCA 1 cut(s) 325
Kzo9I GATC 8 cut(s) 103, 325, 331, 993, 1124, 1172, 1339, 1603
LguI GCTCTTC 1 cut(s) 552
Lsp1109I GCAGC 1 cut(s) 1654
LweI GCATC 4 cut(s) 163, 232, 955, 1306
MaeI CTAG 5 cut(s) 18, 659, 795, 812, 1434
MaeII ACGT 1 cut(s) 576
MaeIII GTNAC 4 cut(s) 294, 341, 680, 1306
MalI GATC 8 cut(s) 105, 327, 333, 995, 1126, 1174, 1341, 1605
MboI GATC 8 cut(s) 103, 325, 331, 993, 1124, 1172, 1339, 1603
MfeI CAATTG 1 cut(s) 1008
MflI RGATCY 1 cut(s) 1603
MhlI GDGCHC 2 cut(s) 582, 783
MlsI TGGCCA 1 cut(s) 351
MluNI TGGCCA 1 cut(s) 351
MlyI GAGTC 4 cut(s) 222, 302, 1035, 1676
MmeI TCCRAC 3 cut(s) 274, 723, 1314
MnlI CCTC 9 cut(s) 183, 487, 535, 654, 947, 1180, 1219, 1412, 1525
Mox20I TGGCCA 1 cut(s) 351
Mph1103I ATGCAT 3 cut(s) 970, 1034, 1321
MroXI GAANNNNTTC 3 cut(s) 63, 1061, 1085
MscI TGGCCA 1 cut(s) 351
MseI TTAA 5 cut(s) 236, 464, 519, 1542, 1589
MslI CAYNNNNRTG 3 cut(s) 309, 774, 1324
Msp20I TGGCCA 1 cut(s) 351
MspA1I CMGCKG 1 cut(s) 1553
MspI CCGG 3 cut(s) 35, 70, 1565
MunI CAATTG 1 cut(s) 1008
Mva1269I GAATGC 1 cut(s) 464
MwoI GCNNNNNNNGC 4 cut(s) 388, 594, 1151, 1443
NdeI CATATG 1 cut(s) 970
NdeII GATC 8 cut(s) 103, 325, 331, 993, 1124, 1172, 1339, 1603
NlaIII CATG 8 cut(s) 314, 332, 398, 1073, 1225, 1297, 1384, 1518
NlaIV GGNNCC 1 cut(s) 39
NmuCI GTSAC 2 cut(s) 294, 341
NsiI ATGCAT 3 cut(s) 970, 1034, 1321
NspV TTCGAA 1 cut(s) 649
PagI TCATGA 2 cut(s) 328, 1069
PciSI GCTCTTC 1 cut(s) 552
PctI GAATGC 1 cut(s) 464
PdmI GAANNNNTTC 3 cut(s) 63, 1061, 1085
PfeI GAWTC 6 cut(s) 122, 437, 719, 912, 1081, 1629
PkrI GCNGC 1 cut(s) 1669
Ple19I CGATCG 1 cut(s) 1175
PleI GAGTC 4 cut(s) 222, 301, 1035, 1676
PmaCI CACGTG 1 cut(s) 577
PmlI CACGTG 1 cut(s) 577
PpsI GAGTC 4 cut(s) 222, 301, 1035, 1676
Ppu21I YACGTR 1 cut(s) 577
Psp124BI GAGCTC 1 cut(s) 783
PspCI CACGTG 1 cut(s) 577
PspN4I GGNNCC 1 cut(s) 39
PspPI GGNCC 1 cut(s) 1189
PstI CTGCAG 1 cut(s) 1672
PsuI RGATCY 1 cut(s) 1603
PvuI CGATCG 1 cut(s) 1175
PvuII CAGCTG 1 cut(s) 1553
RsaI GTAC 5 cut(s) 39, 338, 609, 899, 976
RsaNI GTAC 5 cut(s) 38, 337, 608, 898, 975
RseI CAYNNNNRTG 3 cut(s) 309, 774, 1324
SacI GAGCTC 1 cut(s) 783
SapI GCTCTTC 1 cut(s) 552
SaqAI TTAA 5 cut(s) 236, 464, 519, 1542, 1589
SatI GCNGC 1 cut(s) 1668
Sau3AI GATC 8 cut(s) 103, 325, 331, 993, 1124, 1172, 1339, 1603
Sau96I GGNCC 1 cut(s) 1189
ScaI AGTACT 1 cut(s) 899
SchI GAGTC 4 cut(s) 222, 302, 1035, 1676
SduI GDGCHC 2 cut(s) 582, 783
SfaNI GCATC 4 cut(s) 163, 232, 955, 1306
SfcI CTRYAG 2 cut(s) 1245, 1668
SfuI TTCGAA 1 cut(s) 649
SmiMI CAYNNNNRTG 3 cut(s) 309, 774, 1324
SmlI CTYRAG 2 cut(s) 230, 569
SmoI CTYRAG 2 cut(s) 230, 569
SpeI ACTAGT 1 cut(s) 17
SspI AATATT 1 cut(s) 1062
SspMI CTAG 5 cut(s) 18, 659, 795, 812, 1434
SstI GAGCTC 1 cut(s) 783
TaaI ACNGT 5 cut(s) 181, 341, 946, 1249, 1695
TaiI ACGT 1 cut(s) 579
TaqI TCGA 6 cut(s) 226, 649, 1132, 1171, 1494, 1680
TatI WGTACW 2 cut(s) 607, 897
TfiI GAWTC 6 cut(s) 122, 437, 719, 912, 1081, 1629
Tru1I TTAA 5 cut(s) 236, 464, 519, 1542, 1589
Tru9I TTAA 5 cut(s) 236, 464, 519, 1542, 1589
TscAI CASTG 4 cut(s) 303, 1006, 1692, 1698
TseFI GTSAC 2 cut(s) 294, 341
TseI GCWGC 1 cut(s) 1667
Tsp45I GTSAC 2 cut(s) 294, 341
TspRI CASTG 4 cut(s) 303, 1006, 1692, 1698
XapI RAATTY 2 cut(s) 79, 405
XbaI TCTAGA 1 cut(s) 658
XcmI CCANNNNNNNNNTGG 1 cut(s) 902
XmiI GTMKAC 1 cut(s) 693
XmnI GAANNNNTTC 3 cut(s) 63, 1061, 1085
XspI CTAG 5 cut(s) 18, 659, 795, 812, 1434
ZrmI AGTACT 1 cut(s) 899
Zsp2I ATGCAT 3 cut(s) 970, 1034, 1321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.