MD12G1163000.v1.1

synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000053
Physical Location & Seq
Reverse (-)
24435618 .. 24438777
3160 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1163000.v1.1.491

Sequence Viewer

Length: 1662 bp
ATGTCTTCTCTGGAAGTTCCACTATCTCAATCTTCAAGCCCAAATGACACTTTTGATCCTAAGCCTTCAGCCACTTTTTCTCCCAGCATTTGGGGTGACCATTTTCTGTCCTATGCTTCTTTGGAAGTGGATGTTGAACTTGAACAACATGTACAAGAACTGAAGGAAGAAGTGAGAAGGATGCTAATGACTTCAGTTGAAAATGTTTCACAAAAGCTGAACTTGATTGATGACATTCAGCGCTTAGGTGTGTCCTACCATTTTGGAAATGAGATTGAAGAAATTTTACAGAAAATTCACGAGAACTCATATGATTTGGATGACCTGTACACCACTGCTCTTCATTTTCGACTGCTTAGACAACAAGGTTATAACGTTTCATGCGACTTATTCAACAAATTCAAGGACGGTGATGGAAAATTCAAGGAAACACTCGTTGATGATGTTGTAGGACTACTAAGCTTGTATGAAGCCACACACCTTAGAATACATGGAGAGGAGATACTAGATGAGGCACTAACCTTCACAACCACGCAGCTTGAGTTGACTATATACCGTCTAAGCCCCCCACTTGCAAAAACAGTAACCCATGCCTTAAATCAGCAGCTCCGAAAGGGTTTACCAAGGGTTGAAGCAAGGTATTACTTGTCTGTCTACGAGGAACTAAGAGAATCACCAAATGAAACTCTCCTAACGTTCGCCAAGTTGGATTTCAACCGACTGCAACGAGTCCATCAGAAAGAACTAAGCGAAATCACAAGGTGGTGGAAGGACTTGGACGTGCCAAACAAGCTACCTTTCGCAAGAGACAGATTGGTTGAGGTCTACTTCTGCTGGAGTATGTCTGTCTACTTTCAGCCTCAGTATTCCTTTGCTAGGAGGACATCATGCAAAGTTACCGCTATAACATCCATTATGGATGACATTTATGATACACATGGAAAATTTGAAGAACTAGAGCTCTTTACTGAAGCTATTGAGAGGTGGGATGTCTCAGCTATAGATCAACTACCAGAGTATATGAAACTTTGCTATCGTGCATTGTTGAATATATACAGTGAAATTCATGAAAAGCTTGCGCACGAGGGGAAATTATATCGCATCGACCATGCAAGAGAAGCGATGAAGAACCAAGTTAGAGGCTTCTTCGATGAAGCGAAATTGTTCCGCCAGAATCACATGCCATCATTGGATGAATACATGTCTGTATCACTCATGACCTGTGGCTACCCGTTGCTAATAACCACATCGTTTGTTGGAATGGAAGAAGCTACAATAGACTCCTTTGATTGGTTGTTAACTTCCCCTCAAGCAGTGAAGGCTGCATCAACAGTTACCAGACTTATGGATGACATAGCGGACCATAAGCTTGAGCAAGAGAGAGAACATTTTGCCTCAGCTGTGAACTGTTACATGAGAAAATATGGTGCCACAGAAGAAGAAGCGATAATCGAACTTCGAAGACAAGTGAATAATGCATGGAAGGACATAAACGAAGCGTGCCTCCACCCTACCGCTGTCGCCATGCCCCTGCTAATTCGTATTCTCAATTTTGCTCGGGTCATGGATGTTGTGTACAAGTGCGAAGATGGCTACAATAATGCTGACGGTGGGCTAAAAGATTTTATAGTTTCTACACTAGTGGAACCCGTGGCTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

554

Amino Acids

64.05

Weight (kDa)

5.04

Isoelectric Point (pI)

49.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 30 - 199 1.6e-60 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 257 - 496 5.9e-92 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 298 - 496 4.1e-54 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 372, 1660
Acc16I TGCGCA 1 cut(s) 1080
AccB1I GGYRCC 1 cut(s) 1427
AccB7I CCANNNNNTGG 1 cut(s) 90
AccI GTMKAC 3 cut(s) 654, 825, 849
AciI CCGC 4 cut(s) 900, 1168, 1358, 1515
AclI AACGTT 2 cut(s) 375, 695
AclWI GGATC 1 cut(s) 50
AcsI RAATTY 6 cut(s) 282, 294, 398, 419, 944, 1062
AcuI CTGAAG 4 cut(s) 51, 177, 182, 990
AdeI CACNNNGTG 1 cut(s) 762
AfaI GTAC 3 cut(s) 153, 329, 1577
AfeI AGCGCT 1 cut(s) 242
AfiI CCNNNNNNNGG 3 cut(s) 90, 876, 1290
AflIII ACRYGT 2 cut(s) 148, 1200
AhlI ACTAGT 1 cut(s) 1639
AjiI CACGTC 1 cut(s) 781
AjuI GAANNNNNNNTTGG 2 cut(s) 695, 727
Alw21I GWGCWC 1 cut(s) 963
Alw26I GTCTC 2 cut(s) 801, 997
AlwI GGATC 1 cut(s) 50
Ama87I CYCGRG 1 cut(s) 1557
Aor51HI AGCGCT 1 cut(s) 242
ApeKI GCWGC 3 cut(s) 535, 604, 1322
ApoI RAATTY 6 cut(s) 282, 294, 398, 419, 944, 1062
Asp700I GAANNNNTTC 1 cut(s) 1163
AspLEI GCGC 2 cut(s) 243, 1081
AspS9I GGNCC 1 cut(s) 1360
AsuHPI GGTGA 3 cut(s) 107, 422, 666
AsuII TTCGAA 1 cut(s) 1459
AvaI CYCGRG 1 cut(s) 1557
AvaII GGWCC 1 cut(s) 1360
BanI GGYRCC 1 cut(s) 1427
BanII GRGCYC 1 cut(s) 963
BarI GAAGNNNNNNTAC 2 cut(s) 270, 302
BauI CACGAG 2 cut(s) 299, 1082
BbsI GAAGAC 1 cut(s) 1468
Bbv12I GWGCWC 1 cut(s) 963
BbvCI CCTCAGC 1 cut(s) 1396
BbvI GCAGC 3 cut(s) 547, 616, 1309
BccI CCATC 4 cut(s) 407, 741, 1192, 1583
BcoDI GTCTC 2 cut(s) 801, 997
BcuI ACTAGT 1 cut(s) 1639
BfaI CTAG 4 cut(s) 506, 876, 956, 1640
BfmI CTRYAG 1 cut(s) 999
BfoI RGCGCY 1 cut(s) 244
BisI GCNGC 3 cut(s) 536, 605, 1323
BlsI GCNGC 3 cut(s) 537, 606, 1324
Bme18I GGWCC 1 cut(s) 1360
BmeT110I CYCGRG 1 cut(s) 1557
BmgBI CACGTC 1 cut(s) 781
BmgT120I GGNCC 1 cut(s) 1360
BmiI GGNNCC 2 cut(s) 1429, 1647
BmsI GCATC 3 cut(s) 171, 1110, 1334
BpiI GAAGAC 1 cut(s) 1468
BpmI CTGGAG 1 cut(s) 856
Bpu10I CCTNAGC 3 cut(s) 60, 244, 1396
Bpu14I TTCGAA 1 cut(s) 1459
BpuEI CTTGAG 3 cut(s) 560, 1293, 1391
BsaJI CCNNGG 2 cut(s) 623, 1650
BsaXI ACNNNNNCTCC 2 cut(s) 64, 94
Bsc4I CCNNNNNNNGG 3 cut(s) 90, 876, 1290
BseDI CCNNGG 2 cut(s) 623, 1650
BseGI GGATG 9 cut(s) 136, 186, 325, 908, 925, 994, 1198, 1354, 1573
BseLI CCNNNNNNNGG 3 cut(s) 90, 876, 1290
BseMII CTCAG 3 cut(s) 875, 1008, 1410
BseRI GAGGAG 1 cut(s) 512
BseXI GCAGC 3 cut(s) 547, 616, 1309
BseYI CCCAGC 1 cut(s) 83
BshNI GGYRCC 1 cut(s) 1427
BsiHKAI GWGCWC 1 cut(s) 963
BsiHKCI CYCGRG 1 cut(s) 1557
BslI CCNNNNNNNGG 3 cut(s) 90, 876, 1290
BsmAI GTCTC 2 cut(s) 801, 997
BsoBI CYCGRG 1 cut(s) 1557
Bsp119I TTCGAA 1 cut(s) 1459
Bsp1286I GDGCHC 1 cut(s) 963
Bsp1407I TGTACA 3 cut(s) 151, 327, 1575
Bsp143I GATC 2 cut(s) 55, 1003
BspACI CCGC 4 cut(s) 900, 1168, 1358, 1515
BspCNI CTCAG 3 cut(s) 874, 1007, 1409
BspHI TCATGA 2 cut(s) 1066, 1215
BspLI GGNNCC 2 cut(s) 1429, 1647
BspPI GGATC 1 cut(s) 50
BspQI GCTCTTC 1 cut(s) 345
BspT104I TTCGAA 1 cut(s) 1459
BspT107I GGYRCC 1 cut(s) 1427
BsrGI TGTACA 3 cut(s) 151, 327, 1575
BssECI CCNNGG 2 cut(s) 623, 1650
BssMI GATC 2 cut(s) 55, 1003
BssSI CACGAG 2 cut(s) 299, 1082
BssT1I CCWWGG 1 cut(s) 623
Bst2BI CACGAG 2 cut(s) 299, 1082
Bst4CI ACNGT 7 cut(s) 410, 557, 583, 1058, 1333, 1409, 1610
Bst6I CTCTTC 1 cut(s) 345
BstAUI TGTACA 3 cut(s) 151, 327, 1575
BstBI TTCGAA 1 cut(s) 1459
BstC8I GCNNGC 2 cut(s) 1077, 1501
BstDSI CCRYGG 1 cut(s) 1650
BstEII GGTNACC 1 cut(s) 95
BstENI CCTNNNNNAGG 1 cut(s) 874
BstF5I GGATG 9 cut(s) 136, 186, 325, 908, 925, 994, 1198, 1354, 1573
BstH2I RGCGCY 1 cut(s) 244
BstHHI GCGC 2 cut(s) 243, 1081
BstKTI GATC 2 cut(s) 58, 1006
BstMAI GTCTC 2 cut(s) 801, 997
BstMBI GATC 2 cut(s) 55, 1003
BstMWI GCNNNNNNNGC 4 cut(s) 790, 1118, 1319, 1590
BstNSI RCATGY 3 cut(s) 152, 1183, 1204
BstPI GGTNACC 1 cut(s) 95
BstSFI CTRYAG 1 cut(s) 999
BstV1I GCAGC 3 cut(s) 547, 616, 1309
BstV2I GAAGAC 1 cut(s) 1468
BstXI CCANNNNNNTGG 1 cut(s) 1345
BtgI CCRYGG 1 cut(s) 1650
BtgZI GCGATG 1 cut(s) 1136
BtrI CACGTC 1 cut(s) 781
BtsCI GGATG 9 cut(s) 136, 186, 325, 908, 925, 994, 1198, 1354, 1573
BtsI GCAGTG 2 cut(s) 333, 1320
BtsIMutI CAGTG 3 cut(s) 333, 1063, 1320
Cac8I GCNNGC 2 cut(s) 1077, 1501
CciI TCATGA 2 cut(s) 1066, 1215
CfoI GCGC 2 cut(s) 243, 1081
Cfr13I GGNCC 1 cut(s) 1360
Csp6I GTAC 3 cut(s) 152, 328, 1576
CspCI CAANNNNNGTGG 4 cut(s) 520, 555, 1234, 1269
CviQI GTAC 3 cut(s) 152, 328, 1576
DpnI GATC 2 cut(s) 57, 1005
DpnII GATC 2 cut(s) 55, 1003
DraIII CACNNNGTG 1 cut(s) 762
Eam1104I CTCTTC 1 cut(s) 345
EarI CTCTTC 1 cut(s) 345
EciI GGCGGA 1 cut(s) 1157
Ecl136II GAGCTC 1 cut(s) 961
Eco130I CCWWGG 1 cut(s) 623
Eco24I GRGCYC 1 cut(s) 963
Eco47I GGWCC 1 cut(s) 1360
Eco47III AGCGCT 1 cut(s) 242
Eco53kI GAGCTC 1 cut(s) 961
Eco57I CTGAAG 4 cut(s) 51, 177, 182, 990
Eco88I CYCGRG 1 cut(s) 1557
Eco91I GGTNACC 1 cut(s) 95
EcoICRI GAGCTC 1 cut(s) 961
EcoNI CCTNNNNNAGG 1 cut(s) 874
EcoO65I GGTNACC 1 cut(s) 95
EcoT14I CCWWGG 1 cut(s) 623
EcoT22I ATGCAT 1 cut(s) 1480
EcoT38I GRGCYC 1 cut(s) 963
ErhI CCWWGG 1 cut(s) 623
FalI AAGNNNNNCTT 2 cut(s) 206, 238
FauNDI CATATG 1 cut(s) 310
FblI GTMKAC 3 cut(s) 654, 825, 849
Fnu4HI GCNGC 3 cut(s) 536, 605, 1323
FokI GGATG 9 cut(s) 143, 193, 332, 895, 932, 1001, 1205, 1361, 1580
FriOI GRGCYC 1 cut(s) 963
Fsp4HI GCNGC 3 cut(s) 536, 605, 1323
FspBI CTAG 4 cut(s) 506, 876, 956, 1640
FspI TGCGCA 1 cut(s) 1080
GlaI GCGC 2 cut(s) 242, 1080
GluI GCNGC 3 cut(s) 536, 605, 1323
GsaI CCCAGC 1 cut(s) 87
GsuI CTGGAG 1 cut(s) 856
HaeII RGCGCY 1 cut(s) 244
HhaI GCGC 2 cut(s) 243, 1081
Hin6I GCGC 2 cut(s) 241, 1079
HinP1I GCGC 2 cut(s) 241, 1079
HincII GTYRAC 2 cut(s) 546, 1299
HindII GTYRAC 2 cut(s) 546, 1299
HindIII AAGCTT 3 cut(s) 460, 1073, 1367
HinfI GANTC 4 cut(s) 671, 729, 1174, 1280
HpaI GTTAAC 1 cut(s) 1299
HphI GGTGA 3 cut(s) 107, 422, 666
Hpy166II GTNNAC 9 cut(s) 330, 546, 620, 655, 826, 850, 1299, 1405, 1576
Hpy188I TCNGA 2 cut(s) 611, 738
Hpy188III TCNNGA 4 cut(s) 11, 299, 1067, 1216
Hpy8I GTNNAC 9 cut(s) 330, 546, 620, 655, 826, 850, 1299, 1405, 1576
HpyAV CCTTC 7 cut(s) 75, 157, 171, 532, 763, 1312, 1477
HpyCH4III ACNGT 7 cut(s) 410, 557, 583, 1058, 1333, 1409, 1610
HpyCH4IV ACGT 3 cut(s) 375, 695, 780
HpyCH4V TGCA 7 cut(s) 575, 724, 891, 1040, 1112, 1325, 1478
HpyF10VI GCNNNNNNNGC 4 cut(s) 790, 1118, 1319, 1590
HpySE526I ACGT 3 cut(s) 375, 695, 780
HspAI GCGC 2 cut(s) 241, 1079
KspAI GTTAAC 1 cut(s) 1299
Kzo9I GATC 2 cut(s) 55, 1003
LguI GCTCTTC 1 cut(s) 345
LmnI GCTCC 1 cut(s) 612
LpnPI CCDG 8 cut(s) 97, 338, 820, 1026, 1184, 1234, 1351, 1544
Lsp1109I GCAGC 3 cut(s) 547, 616, 1309
LweI GCATC 3 cut(s) 171, 1110, 1334
MaeI CTAG 4 cut(s) 506, 876, 956, 1640
MaeII ACGT 3 cut(s) 375, 695, 780
MaeIII GTNAC 5 cut(s) 95, 583, 895, 1333, 1409
MalI GATC 2 cut(s) 57, 1005
MboI GATC 2 cut(s) 55, 1003
MhlI GDGCHC 1 cut(s) 963
MlyI GAGTC 2 cut(s) 738, 1274
MmeI TCCRAC 2 cut(s) 687, 1237
Mph1103I ATGCAT 1 cut(s) 1480
MroXI GAANNNNTTC 1 cut(s) 1163
MseI TTAA 2 cut(s) 596, 1298
MspA1I CMGCKG 2 cut(s) 1400, 1517
MwoI GCNNNNNNNGC 4 cut(s) 790, 1118, 1319, 1590
NdeI CATATG 1 cut(s) 310
NdeII GATC 2 cut(s) 55, 1003
NlaIV GGNNCC 2 cut(s) 1429, 1647
NmuCI GTSAC 1 cut(s) 95
NsbI TGCGCA 1 cut(s) 1080
NsiI ATGCAT 1 cut(s) 1480
NspI RCATGY 3 cut(s) 152, 1183, 1204
NspV TTCGAA 1 cut(s) 1459
PagI TCATGA 2 cut(s) 1066, 1215
PciI ACATGT 2 cut(s) 148, 1200
PciSI GCTCTTC 1 cut(s) 345
PcsI WCGNNNNNNNCGW 1 cut(s) 381
PdmI GAANNNNTTC 1 cut(s) 1163
PfeI GAWTC 2 cut(s) 671, 1174
PflMI CCANNNNNTGG 1 cut(s) 90
PkrI GCNGC 3 cut(s) 537, 606, 1324
PleI GAGTC 2 cut(s) 737, 1274
PpsI GAGTC 2 cut(s) 737, 1274
PscI ACATGT 2 cut(s) 148, 1200
PsiI TTATAA 2 cut(s) 372, 1660
Psp124BI GAGCTC 1 cut(s) 963
Psp1406I AACGTT 2 cut(s) 375, 695
PspEI GGTNACC 1 cut(s) 95
PspFI CCCAGC 1 cut(s) 83
PspN4I GGNNCC 2 cut(s) 1429, 1647
PspPI GGNCC 1 cut(s) 1360
PsrI GAACNNNNNNTAC 2 cut(s) 135, 167
PvuII CAGCTG 1 cut(s) 1400
RsaI GTAC 3 cut(s) 153, 329, 1577
RsaNI GTAC 3 cut(s) 152, 328, 1576
SacI GAGCTC 1 cut(s) 963
SapI GCTCTTC 1 cut(s) 345
SaqAI TTAA 2 cut(s) 596, 1298
SatI GCNGC 3 cut(s) 536, 605, 1323
Sau3AI GATC 2 cut(s) 55, 1003
Sau96I GGNCC 1 cut(s) 1360
SchI GAGTC 2 cut(s) 738, 1274
SduI GDGCHC 1 cut(s) 963
SfaNI GCATC 3 cut(s) 171, 1110, 1334
SfcI CTRYAG 1 cut(s) 999
SfuI TTCGAA 1 cut(s) 1459
SinI GGWCC 1 cut(s) 1360
SmlI CTYRAG 3 cut(s) 539, 1308, 1370
SmoI CTYRAG 3 cut(s) 539, 1308, 1370
SpeI ACTAGT 1 cut(s) 1639
SsiI CCGC 4 cut(s) 900, 1168, 1358, 1515
SspMI CTAG 4 cut(s) 506, 876, 956, 1640
SstI GAGCTC 1 cut(s) 963
StyI CCWWGG 1 cut(s) 623
TaaI ACNGT 7 cut(s) 410, 557, 583, 1058, 1333, 1409, 1610
TaiI ACGT 3 cut(s) 378, 698, 783
TaqI TCGA 5 cut(s) 349, 1104, 1149, 1452, 1459
TatI WGTACW 3 cut(s) 151, 327, 1575
TfiI GAWTC 2 cut(s) 671, 1174
Tru1I TTAA 2 cut(s) 596, 1298
Tru9I TTAA 2 cut(s) 596, 1298
TscAI CASTG 3 cut(s) 340, 1063, 1320
TseFI GTSAC 1 cut(s) 95
TseI GCWGC 3 cut(s) 535, 604, 1322
Tsp45I GTSAC 1 cut(s) 95
TspRI CASTG 3 cut(s) 340, 1063, 1320
Van91I CCANNNNNTGG 1 cut(s) 90
VpaK11BI GGWCC 1 cut(s) 1360
XagI CCTNNNNNAGG 1 cut(s) 874
XapI RAATTY 6 cut(s) 282, 294, 398, 419, 944, 1062
XceI RCATGY 3 cut(s) 152, 1183, 1204
XmiI GTMKAC 3 cut(s) 654, 825, 849
XmnI GAANNNNTTC 1 cut(s) 1163
XspI CTAG 4 cut(s) 506, 876, 956, 1640
Zsp2I ATGCAT 1 cut(s) 1480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.