Prupe.4G194400_v2.0.a1

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000053
Physical Location & Seq
Forward (+)
11801075 .. 11803032
1958 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G194400.1

Sequence Viewer

Length: 1374 bp
ATGTCTGTCCAAGTTGGTTCAGCAGCTCAACCACAAAATGCTAAGCCAGATGAAATTGCTCGTAGGACAGCAAATTTTCACCCAAGCATTTGGGGAGATCAGTTCATCAATTATGATGATTCACAAGACATGATAACTCATGCTCATAAGCAAGGGCAGGTTGACGAACTAAGAGAAGTTGTGAGGAGGGAAGTATTCACAACTAGTGCAGGTGATCTTTCACATCAGCTCAAGCTAATCGATGCAATCCAGCGCCTTGGCGTGGCATACCATTTCGAAAGAGAAATAGAAGAAGCACTGGAATGCATGCATACTACACTTCATGACCATGACAGTGATGATGATGGTGATCTATACAATGTTGCTCTTTGTTTTCGACTACTCAGACAACATGGACATAATGTTTCATGTGATATATTCAACAAGTTCAAAGATGAAAATGGTAGCTTCAAAGAAAGCTTAATTGCTGATATGTCTGGCATGCTAAGCTTTTATGAAGCAACACATCTGAGGTCCGCAACAACCCCTGTAAGCAGTCCATTGGCTGCACAAATAACTCAGGCCTTAGCGAGACCTCTGAGAAAAACTCTAGAGAGATTAGGTGCCAGGCGTTACATATCGATCTACCAAGACGAAGCTTCACATAATGAGTGCTTGTTGAAACTTGCAAAGTTAGATTTCAATCTTGTTCAGCCTTTACACAAAAAGGAGCTACAAGATATTACTAGGTGGTGTCGAGCACTTGACCTTGAAAGGAAGCTGCCATTTGCAAGAGATAGGATGGTGGAGTTGTACTTTTGGATAGTGGGAGTGTATTTTGAGCCTCAGTATTCTGTGGGGAGAAAAATTATGACGAAAGTGAGTGTCTTGCTAACGATATTGGATGATATCTACGATGCATTTGGCACATTTGAAGAACTAGTGATCTTTACTGAAGCAATTGACAGGTGGGATCTCAATTGCAGTAATGAACTCCCGGACTACATGAAAATATTTTACCAGGCACTCTTGAATCTTTTCAATGAAATTGAGGAGGAGATGGTCAAGGAAGGAAGATCATACCGAGTTCCTTATGCAATACAAGCTATGAAAGATCAAGCTAGATCCTATTTCAATGAGGCCCGGTGGCTCCACGAAGGCCGAATTCCAAGCATGGAGGAGTATATGAGTGTTGCAACGGTTTCTATAAGTTACACCTTCCTTACAACCATCTCTTTACTTGGCATGGGAGATATTGTAACGAATGAGTCATTTGAGTGGTTGCTCAATGACCCCAAAATTGTCAGAGCTGCAAACACTATTTTCAGGCTCATGGATGACATTATTTCGACCAACATGCTAAAATATGAATATGGTTTGAATGTATTAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

458

Amino Acids

52.82

Weight (kDa)

5.17

Isoelectric Point (pI)

47.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 200
Acc36I ACCTGC 2 cut(s) 148, 200
AccB1I GGYRCC 1 cut(s) 602
AciI CCGC 1 cut(s) 516
AclWI GGATC 2 cut(s) 960, 1098
AcsI RAATTY 2 cut(s) 73, 1143
AcuI CTGAAG 1 cut(s) 954
AfaI GTAC 1 cut(s) 794
AfiI CCNNNNNNNGG 1 cut(s) 262
AhlI ACTAGT 2 cut(s) 203, 919
AjnI CCWGG 2 cut(s) 605, 999
Alw21I GWGCWC 1 cut(s) 742
Alw26I GTCTC 1 cut(s) 565
AlwI GGATC 2 cut(s) 960, 1098
AoxI GGCC 3 cut(s) 561, 1119, 1138
ApeKI GCWGC 4 cut(s) 23, 545, 760, 1289
ApoI RAATTY 2 cut(s) 73, 1143
Asp700I GAANNNNTTC 1 cut(s) 1016
AspLEI GCGC 1 cut(s) 255
AspS9I GGNCC 2 cut(s) 513, 1120
AsuC2I CCSGG 2 cut(s) 977, 1123
AsuHPI GGTGA 3 cut(s) 71, 224, 359
AsuII TTCGAA 1 cut(s) 276
AvaII GGWCC 1 cut(s) 513
BanI GGYRCC 1 cut(s) 602
Bbv12I GWGCWC 1 cut(s) 742
BbvI GCAGC 4 cut(s) 35, 532, 747, 1276
BccI CCATC 4 cut(s) 338, 775, 1033, 1217
BcgI CGANNNNNNTGC 2 cut(s) 1318, 1352
BciT130I CCWGG 2 cut(s) 607, 1001
BcnI CCSGG 2 cut(s) 977, 1123
BcoDI GTCTC 1 cut(s) 565
BcuI ACTAGT 2 cut(s) 203, 919
BfaI CTAG 5 cut(s) 204, 590, 726, 920, 1101
BfoI RGCGCY 1 cut(s) 256
BfuAI ACCTGC 2 cut(s) 148, 200
BisI GCNGC 4 cut(s) 24, 546, 761, 1290
BlpI GCTNAGC 2 cut(s) 42, 485
BlsI GCNGC 4 cut(s) 25, 547, 762, 1291
Bme1390I CCNGG 4 cut(s) 607, 977, 1001, 1123
Bme18I GGWCC 1 cut(s) 513
BmgT120I GGNCC 2 cut(s) 513, 1120
BmiI GGNNCC 2 cut(s) 604, 1130
BmrFI CCNGG 4 cut(s) 607, 977, 1001, 1123
BmsI GCATC 2 cut(s) 232, 886
BplI GAGNNNNNCTC 2 cut(s) 571, 603
Bpu10I CCTNAGC 1 cut(s) 565
Bpu1102I GCTNAGC 2 cut(s) 42, 485
Bpu14I TTCGAA 1 cut(s) 276
BpuEI CTTGAG 1 cut(s) 215
BpuMI CCSGG 2 cut(s) 977, 1123
Bsa29I ATCGAT 2 cut(s) 240, 620
BsaBI GATNNNNATC 2 cut(s) 348, 681
BsaI GGTCTC 1 cut(s) 565
BsaJI CCNNGG 1 cut(s) 256
BsaXI ACNNNNNCTCC 2 cut(s) 1221, 1251
Bsc4I CCNNNNNNNGG 1 cut(s) 262
Bse1I ACTGG 1 cut(s) 303
Bse8I GATNNNNATC 2 cut(s) 348, 681
BseBI CCWGG 2 cut(s) 607, 1001
BseCI ATCGAT 2 cut(s) 240, 620
BseDI CCNNGG 1 cut(s) 256
BseGI GGATG 3 cut(s) 786, 889, 1321
BseJI GATNNNNATC 2 cut(s) 348, 681
BseLI CCNNNNNNNGG 1 cut(s) 262
BseMII CTCAG 5 cut(s) 397, 500, 569, 572, 839
BseNI ACTGG 1 cut(s) 303
BseRI GAGGAG 4 cut(s) 199, 1046, 1049, 1172
BseXI GCAGC 4 cut(s) 35, 532, 747, 1276
BsgI GTGCAG 2 cut(s) 228, 531
BshFI GGCC 3 cut(s) 563, 1121, 1140
BshNI GGYRCC 1 cut(s) 602
BshVI ATCGAT 2 cut(s) 240, 620
BsiHKAI GWGCWC 1 cut(s) 742
BsiSI CCGG 2 cut(s) 977, 1123
BslI CCNNNNNNNGG 1 cut(s) 262
BsmAI GTCTC 1 cut(s) 565
BsmI GAATGC 1 cut(s) 308
BsnI GGCC 3 cut(s) 563, 1121, 1140
Bso31I GGTCTC 1 cut(s) 565
Bsp119I TTCGAA 1 cut(s) 276
Bsp1286I GDGCHC 1 cut(s) 742
Bsp143I GATC 9 cut(s) 97, 214, 349, 621, 924, 952, 1055, 1093, 1103
Bsp1720I GCTNAGC 2 cut(s) 42, 485
BspACI CCGC 1 cut(s) 516
BspANI GGCC 3 cut(s) 563, 1121, 1140
BspCNI CTCAG 5 cut(s) 396, 501, 570, 571, 838
BspDI ATCGAT 2 cut(s) 240, 620
BspHI TCATGA 1 cut(s) 322
BspLI GGNNCC 2 cut(s) 604, 1130
BspMI ACCTGC 2 cut(s) 148, 200
BspPI GGATC 2 cut(s) 960, 1098
BspT104I TTCGAA 1 cut(s) 276
BspT107I GGYRCC 1 cut(s) 602
BspTNI GGTCTC 1 cut(s) 565
BsrI ACTGG 1 cut(s) 303
BssECI CCNNGG 1 cut(s) 256
BssMI GATC 9 cut(s) 97, 214, 349, 621, 924, 952, 1055, 1093, 1103
BssT1I CCWWGG 1 cut(s) 256
Bst2UI CCWGG 2 cut(s) 607, 1001
Bst4CI ACNGT 2 cut(s) 335, 1180
BstBI TTCGAA 1 cut(s) 276
BstC8I GCNNGC 2 cut(s) 308, 482
BstDEI CTNAG 9 cut(s) 42, 170, 383, 485, 509, 558, 565, 578, 825
BstF5I GGATG 3 cut(s) 786, 889, 1321
BstH2I RGCGCY 1 cut(s) 256
BstHHI GCGC 1 cut(s) 255
BstKTI GATC 9 cut(s) 100, 217, 352, 624, 927, 955, 1058, 1096, 1106
BstMAI GTCTC 1 cut(s) 565
BstMBI GATC 9 cut(s) 97, 214, 349, 621, 924, 952, 1055, 1093, 1103
BstMWI GCNNNNNNNGC 2 cut(s) 486, 1082
BstNI CCWGG 2 cut(s) 607, 1001
BstNSI RCATGY 3 cut(s) 310, 484, 1339
BstSCI CCNGG 4 cut(s) 605, 975, 999, 1121
BstV1I GCAGC 4 cut(s) 35, 532, 747, 1276
BstX2I RGATCY 2 cut(s) 952, 1103
BstXI CCANNNNNNTGG 2 cut(s) 90, 257
BstYI RGATCY 2 cut(s) 952, 1103
Bsu15I ATCGAT 2 cut(s) 240, 620
BsuRI GGCC 3 cut(s) 563, 1121, 1140
BsuTUI ATCGAT 2 cut(s) 240, 620
BtsCI GGATG 3 cut(s) 786, 889, 1321
BtsIMutI CAGTG 2 cut(s) 296, 340
BveI ACCTGC 2 cut(s) 148, 200
Cac8I GCNNGC 2 cut(s) 308, 482
CciI TCATGA 1 cut(s) 322
CfoI GCGC 1 cut(s) 255
Cfr13I GGNCC 2 cut(s) 513, 1120
ClaI ATCGAT 2 cut(s) 240, 620
Csp6I GTAC 1 cut(s) 793
CviQI GTAC 1 cut(s) 793
DdeI CTNAG 9 cut(s) 42, 170, 383, 485, 509, 558, 565, 578, 825
DpnI GATC 9 cut(s) 99, 216, 351, 623, 926, 954, 1057, 1095, 1105
DpnII GATC 9 cut(s) 97, 214, 349, 621, 924, 952, 1055, 1093, 1103
Eco130I CCWWGG 1 cut(s) 256
Eco147I AGGCCT 1 cut(s) 563
Eco31I GGTCTC 1 cut(s) 565
Eco32I GATATC 1 cut(s) 889
Eco47I GGWCC 1 cut(s) 513
Eco57I CTGAAG 1 cut(s) 954
EcoRI GAATTC 1 cut(s) 1143
EcoRII CCWGG 2 cut(s) 605, 999
EcoRV GATATC 1 cut(s) 889
EcoT14I CCWWGG 1 cut(s) 256
EcoT22I ATGCAT 3 cut(s) 308, 312, 901
ErhI CCWWGG 1 cut(s) 256
Fnu4HI GCNGC 4 cut(s) 24, 546, 761, 1290
FokI GGATG 3 cut(s) 793, 896, 1328
Fsp4HI GCNGC 4 cut(s) 24, 546, 761, 1290
FspBI CTAG 5 cut(s) 204, 590, 726, 920, 1101
GlaI GCGC 1 cut(s) 254
GluI GCNGC 4 cut(s) 24, 546, 761, 1290
HaeII RGCGCY 1 cut(s) 256
HaeIII GGCC 3 cut(s) 563, 1121, 1140
HapII CCGG 2 cut(s) 977, 1123
HhaI GCGC 1 cut(s) 255
Hin6I GCGC 1 cut(s) 253
HinP1I GCGC 1 cut(s) 253
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HindIII AAGCTT 3 cut(s) 457, 487, 636
HinfI GANTC 3 cut(s) 119, 1012, 1247
HpaII CCGG 2 cut(s) 977, 1123
HphI GGTGA 3 cut(s) 71, 224, 359
Hpy166II GTNNAC 1 cut(s) 163
Hpy188I TCNGA 4 cut(s) 386, 510, 579, 1286
Hpy188III TCNNGA 3 cut(s) 323, 590, 1009
Hpy8I GTNNAC 1 cut(s) 163
HpyAV CCTTC 3 cut(s) 1043, 1130, 1207
HpyCH4III ACNGT 2 cut(s) 335, 1180
HpyF10VI GCNNNNNNNGC 2 cut(s) 486, 1082
HpyF3I CTNAG 9 cut(s) 42, 170, 383, 485, 509, 558, 565, 578, 825
HspAI GCGC 1 cut(s) 253
Kzo9I GATC 9 cut(s) 97, 214, 349, 621, 924, 952, 1055, 1093, 1103
LmnI GCTCC 2 cut(s) 709, 1134
Lsp1109I GCAGC 4 cut(s) 35, 532, 747, 1276
LweI GCATC 2 cut(s) 232, 886
MaeI CTAG 5 cut(s) 204, 590, 726, 920, 1101
MaeIII GTNAC 3 cut(s) 611, 1190, 1237
MalI GATC 9 cut(s) 99, 216, 351, 623, 926, 954, 1057, 1095, 1105
MboI GATC 9 cut(s) 97, 214, 349, 621, 924, 952, 1055, 1093, 1103
MboII GAAGA 3 cut(s) 302, 926, 1065
MfeI CAATTG 2 cut(s) 939, 958
MflI RGATCY 2 cut(s) 952, 1103
MhlI GDGCHC 1 cut(s) 742
MlyI GAGTC 1 cut(s) 1256
MnlI CCTC 9 cut(s) 177, 180, 504, 585, 834, 1024, 1027, 1111, 1150
Mph1103I ATGCAT 3 cut(s) 308, 312, 901
MroXI GAANNNNTTC 1 cut(s) 1016
MseI TTAA 2 cut(s) 461, 1372
MslI CAYNNNNRTG 4 cut(s) 301, 327, 333, 1255
MspI CCGG 2 cut(s) 977, 1123
MspR9I CCNGG 4 cut(s) 607, 977, 1001, 1123
MunI CAATTG 2 cut(s) 939, 958
Mva1269I GAATGC 1 cut(s) 308
MvaI CCWGG 2 cut(s) 607, 1001
MwoI GCNNNNNNNGC 2 cut(s) 486, 1082
NciI CCSGG 2 cut(s) 977, 1123
NdeII GATC 9 cut(s) 97, 214, 349, 621, 924, 952, 1055, 1093, 1103
NlaIV GGNNCC 2 cut(s) 604, 1130
NsiI ATGCAT 3 cut(s) 308, 312, 901
NspI RCATGY 3 cut(s) 310, 484, 1339
NspV TTCGAA 1 cut(s) 276
PaeI GCATGC 2 cut(s) 310, 484
PagI TCATGA 1 cut(s) 322
PaqCI CACCTGC 1 cut(s) 200
PceI AGGCCT 1 cut(s) 563
PctI GAATGC 1 cut(s) 308
PdmI GAANNNNTTC 1 cut(s) 1016
PfeI GAWTC 2 cut(s) 119, 1012
PfoI TCCNGGA 1 cut(s) 975
PkrI GCNGC 4 cut(s) 25, 547, 762, 1291
PleI GAGTC 1 cut(s) 1255
PpsI GAGTC 1 cut(s) 1255
Psp6I CCWGG 2 cut(s) 605, 999
PspGI CCWGG 2 cut(s) 605, 999
PspN4I GGNNCC 2 cut(s) 604, 1130
PspPI GGNCC 2 cut(s) 513, 1120
PsuI RGATCY 2 cut(s) 952, 1103
RsaI GTAC 1 cut(s) 794
RsaNI GTAC 1 cut(s) 793
RseI CAYNNNNRTG 4 cut(s) 301, 327, 333, 1255
SaqAI TTAA 2 cut(s) 461, 1372
SatI GCNGC 4 cut(s) 24, 546, 761, 1290
Sau3AI GATC 9 cut(s) 97, 214, 349, 621, 924, 952, 1055, 1093, 1103
Sau96I GGNCC 2 cut(s) 513, 1120
SchI GAGTC 1 cut(s) 1256
ScrFI CCNGG 4 cut(s) 607, 977, 1001, 1123
SduI GDGCHC 1 cut(s) 742
SfaNI GCATC 2 cut(s) 232, 886
SfuI TTCGAA 1 cut(s) 276
SinI GGWCC 1 cut(s) 513
SmiMI CAYNNNNRTG 4 cut(s) 301, 327, 333, 1255
SmlI CTYRAG 1 cut(s) 230
SmoI CTYRAG 1 cut(s) 230
SpeI ACTAGT 2 cut(s) 203, 919
SphI GCATGC 2 cut(s) 310, 484
SseBI AGGCCT 1 cut(s) 563
SsiI CCGC 1 cut(s) 516
SspI AATATT 1 cut(s) 993
SspMI CTAG 5 cut(s) 204, 590, 726, 920, 1101
StuI AGGCCT 1 cut(s) 563
StyD4I CCNGG 4 cut(s) 605, 975, 999, 1121
StyI CCWWGG 1 cut(s) 256
TaaI ACNGT 2 cut(s) 335, 1180
TaqI TCGA 6 cut(s) 240, 276, 376, 620, 736, 1328
TatI WGTACW 1 cut(s) 792
TfiI GAWTC 2 cut(s) 119, 1012
Tru1I TTAA 2 cut(s) 461, 1372
Tru9I TTAA 2 cut(s) 461, 1372
TscAI CASTG 2 cut(s) 303, 340
TseI GCWGC 4 cut(s) 23, 545, 760, 1289
TspRI CASTG 2 cut(s) 303, 340
VpaK11BI GGWCC 1 cut(s) 513
XapI RAATTY 2 cut(s) 73, 1143
XbaI TCTAGA 1 cut(s) 589
XceI RCATGY 3 cut(s) 310, 484, 1339
XmnI GAANNNNTTC 1 cut(s) 1016
XspI CTAG 5 cut(s) 204, 590, 726, 920, 1101
Zsp2I ATGCAT 3 cut(s) 308, 312, 901
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.