FvH4_5g35700

(-)-germacrene D synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000053
Physical Location & Seq
Forward (+)
26148083 .. 26151136
3054 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g35700.t1

Sequence Viewer

Length: 1665 bp
ATGTCTCTTCAAGTTTTAGCATCTCAATCTCAAACGCCAAATGCCAGGCCTACAGCTAATTACACTCCAAGCATTTGGGGAGATCACTTTCTCTCTTACGCTACGGCGGAAGTGGACTTCAGTCACAAGCAACAATTCCAAGAACTCAAAAGACAAGTGAAAATGATGCTAATGGTTCCAGTGAAAGAGCCTTCAGAAAAATTGAACTTAGTTGATGACATTCAGCGCCTAGGAGTGTCCTATCATTTTGAAAATGAGATTGACGAAGTTCTGGAACAAATTTATCAGGCCACTCACGGTAATGATATGGAAAATGATGATGATCTTTATACTACTGCTTTGCGTTTTCGGTTGCTTAGACAACAAGGTTATAATGTTTCTTGCGATATATTCAATAAGTTCAAGGAAGGAAGTGATCAGACATTTAAGCAAACTCTTCAGAGTGATGTATCAGGACTATTAAGCTTGTACGAAGCCACCCATCTCAGGATACACGGAGAAGACATACTCGAGGAGGCACTAGACTTCACCACCACTCATCTTGAGTCGATCAAACACAGTTTAAGCCACTCACTCTCAAAACAAGTATCTCATTCCTTGAATCAGCCACTTCGAAAGGGCATCATCAGGGTGGAAGCAAGATATTACTTATCGATCTACCACGAATGTGATTCACATAATGAAACTCTCCTGACATTTGCCAAGTTGGATTTCAACCTGTTGCAGCAAGTTCATCAAATAGAACTTTGTGAAATTACAAGGTGGTGGAAGGACTTGGATGTGAGAAACGAGCTGCCGTTTACAAGAGATAGAGTTACTGAGGTCTACTTCTGCTGGGCTTTATCAATCTGCTTTGAACCTGAATATGCCTTTGCTAGGAGGACTTTATGCAAAGTTACTGCCCTGACCTCCATTCTCGATGACATTTATGACTTGTATGGTACACTCGAAGAACTAGAGCTCTTTACTGAAGCTATTGAGAGGTGGGATATTCGTGTCATCGATGCACTGCCAAACTATATGAAAGTCTGTTATAAGGCACTGTTAGACGTGTATACTGAAGTTGAAGAAGAGCTTGCAAAGGAGAGGAAGTTGTATCTGATCCACTATGCCAGAGAAGCTATGAAACTGCAAGCTAGAAGTTACTATCTTGAAGCCAAATGGTTCCACCAGAAATACATACCGACAATGGATGAATACATTGCCCTATCATCACTGACCTCAGGCTATCCACTGCTTATAACCACCACTTTTGTTACCATGGAAGAAATTACTACACAAGACCCATTTGACTGGTTGGCCACTTATCCCAAATCTGTGAAGGGGTCTGCAGTAGTTGCCCGACTTATGGACGACTTGGTTTCCCACAAGTTTGAGCAAAAGAGAGGACATGTTGCATCAGCTGTGGAGTGCTACATGAAAGAATTTGGAGCCACAGAAGAAGAAACAATAATCGAATTGCGTAGTAAAGTGAGTGACGCATGGAAGGACATAAACGAATCGTTCCTGGTCCCTAATTATGTTTCAAGGCCACTGCTGACTCGAATTCTTAATTTTGCATGTGTTATGGATGTTGTTTACAAGTATGAAGATGGTTATACTACTCAGCATGGCATGCTCAAGGATCTGATAACTTCTGTACTTATCGAACAAGTGCCTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

555

Amino Acids

64.57

Weight (kDa)

5.09

Isoelectric Point (pI)

50.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 25 - 200 1.1e-60 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 257 - 497 1.4e-91 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 298 - 497 8.5e-58 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 372, 1035, 1241
AccI GTMKAC 2 cut(s) 825, 1055
AciI CCGC 1 cut(s) 107
AclWI GGATC 2 cut(s) 1096, 1632
AcoI YGGCCR 1 cut(s) 1299
AcsI RAATTY 3 cut(s) 279, 1424, 1545
AcuI CTGAAG 5 cut(s) 103, 177, 422, 990, 1080
AfaI GTAC 3 cut(s) 470, 943, 1641
AfiI CCNNNNNNNGG 3 cut(s) 486, 876, 1348
AflIII ACRYGT 2 cut(s) 1050, 1390
AjiI CACGTC 1 cut(s) 1051
AjnI CCWGG 2 cut(s) 44, 1506
AjuI GAANNNNNNNTTGG 2 cut(s) 695, 727
AleI CACNNNNGTG 1 cut(s) 666
AluBI AGCT 9 cut(s) 56, 465, 793, 961, 974, 1075, 1121, 1136, 1403
AluI AGCT 9 cut(s) 56, 465, 793, 961, 974, 1075, 1121, 1136, 1403
Alw21I GWGCWC 1 cut(s) 963
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 2 cut(s) 1096, 1632
Ama87I CYCGRG 1 cut(s) 509
AoxI GGCC 4 cut(s) 47, 288, 1299, 1529
ApeKI GCWGC 2 cut(s) 724, 793
ApoI RAATTY 3 cut(s) 279, 1424, 1545
AspA2I CCTAGG 1 cut(s) 229
AspLEI GCGC 1 cut(s) 228
AspS9I GGNCC 1 cut(s) 1510
AsuHPI GGTGA 1 cut(s) 520
AsuII TTCGAA 1 cut(s) 613
AvaI CYCGRG 1 cut(s) 509
AvaII GGWCC 1 cut(s) 1510
AvrII CCTAGG 1 cut(s) 229
AxyI CCTNAGG 1 cut(s) 1222
BalI TGGCCA 1 cut(s) 1301
BanII GRGCYC 1 cut(s) 963
BbsI GAAGAC 1 cut(s) 507
Bbv12I GWGCWC 1 cut(s) 963
BbvI GCAGC 2 cut(s) 736, 780
BccI CCATC 2 cut(s) 489, 1586
BceAI ACGGC 2 cut(s) 120, 781
BciT130I CCWGG 2 cut(s) 46, 1508
BciVI GTATCC 1 cut(s) 483
BclI TGATCA 1 cut(s) 415
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 5 cut(s) 230, 521, 876, 956, 1137
BfmI CTRYAG 2 cut(s) 51, 1329
BfoI RGCGCY 1 cut(s) 229
BfuI GTATCC 1 cut(s) 483
BisI GCNGC 2 cut(s) 725, 794
BlnI CCTAGG 1 cut(s) 229
BlsI GCNGC 2 cut(s) 726, 795
Bme1390I CCNGG 2 cut(s) 46, 1508
Bme18I GGWCC 1 cut(s) 1510
BmeT110I CYCGRG 1 cut(s) 509
BmgBI CACGTC 1 cut(s) 1051
BmgT120I GGNCC 1 cut(s) 1510
BmiI GGNNCC 4 cut(s) 177, 1166, 1432, 1512
BmrFI CCNGG 2 cut(s) 46, 1508
BmsI GCATC 5 cut(s) 29, 156, 630, 994, 1406
BoxI GACNNNNGTC 1 cut(s) 120
BpiI GAAGAC 1 cut(s) 507
Bpu14I TTCGAA 1 cut(s) 613
BpuEI CTTGAG 2 cut(s) 563, 1604
Bsa29I ATCGAT 2 cut(s) 653, 1002
BsaBI GATNNNNATC 1 cut(s) 321
BsaJI CCNNGG 2 cut(s) 229, 1260
BsaXI ACNNNNNCTCC 2 cut(s) 1076, 1106
Bsc4I CCNNNNNNNGG 3 cut(s) 486, 876, 1348
Bse1I ACTGG 2 cut(s) 179, 1298
Bse21I CCTNAGG 1 cut(s) 1222
Bse3DI GCAATG 1 cut(s) 1200
Bse8I GATNNNNATC 1 cut(s) 321
BseBI CCWGG 2 cut(s) 46, 1508
BseCI ATCGAT 2 cut(s) 653, 1002
BseDI CCNNGG 2 cut(s) 229, 1260
BseGI GGATG 3 cut(s) 784, 1198, 1576
BseJI GATNNNNATC 1 cut(s) 321
BseLI CCNNNNNNNGG 3 cut(s) 486, 876, 1348
BseMI GCAATG 1 cut(s) 1200
BseMII CTCAG 4 cut(s) 499, 810, 1236, 1619
BseNI ACTGG 2 cut(s) 179, 1298
BseRI GAGGAG 1 cut(s) 527
BseXI GCAGC 2 cut(s) 736, 780
BseYI CCCAGC 1 cut(s) 834
BshFI GGCC 4 cut(s) 49, 290, 1301, 1531
BshVI ATCGAT 2 cut(s) 653, 1002
BsiHKAI GWGCWC 1 cut(s) 963
BsiHKCI CYCGRG 1 cut(s) 509
BslFI GGGAC 1 cut(s) 1496
BslI CCNNNNNNNGG 3 cut(s) 486, 876, 1348
BsmAI GTCTC 1 cut(s) 9
BsmFI GGGAC 1 cut(s) 1496
BsnI GGCC 4 cut(s) 49, 290, 1301, 1531
BsoBI CYCGRG 1 cut(s) 509
Bsp119I TTCGAA 1 cut(s) 613
Bsp1286I GDGCHC 1 cut(s) 963
Bsp143I GATC 7 cut(s) 82, 322, 415, 549, 654, 1101, 1624
Bsp19I CCATGG 1 cut(s) 1260
BspACI CCGC 1 cut(s) 107
BspANI GGCC 4 cut(s) 49, 290, 1301, 1531
BspCNI CTCAG 4 cut(s) 498, 811, 1235, 1618
BspDI ATCGAT 2 cut(s) 653, 1002
BspLI GGNNCC 4 cut(s) 177, 1166, 1432, 1512
BspMAI CTGCAG 1 cut(s) 1333
BspPI GGATC 2 cut(s) 1096, 1632
BspQI GCTCTTC 1 cut(s) 1065
BspT104I TTCGAA 1 cut(s) 613
BsrDI GCAATG 1 cut(s) 1200
BsrI ACTGG 2 cut(s) 179, 1298
BssECI CCNNGG 2 cut(s) 229, 1260
BssMI GATC 7 cut(s) 82, 322, 415, 549, 654, 1101, 1624
BssNAI GTATAC 1 cut(s) 1056
BssT1I CCWWGG 2 cut(s) 229, 1260
Bst1107I GTATAC 1 cut(s) 1056
Bst2UI CCWGG 2 cut(s) 46, 1508
Bst4CI ACNGT 3 cut(s) 299, 560, 1044
Bst6I CTCTTC 3 cut(s) 12, 441, 1065
BstAPI GCANNNNNTGC 2 cut(s) 1337, 1615
BstBI TTCGAA 1 cut(s) 613
BstC8I GCNNGC 3 cut(s) 1077, 1134, 1616
BstDEI CTNAG 6 cut(s) 208, 356, 485, 819, 1222, 1605
BstDSI CCRYGG 1 cut(s) 1260
BstENI CCTNNNNNAGG 1 cut(s) 874
BstF5I GGATG 3 cut(s) 784, 1198, 1576
BstH2I RGCGCY 1 cut(s) 229
BstHHI GCGC 1 cut(s) 228
BstKTI GATC 7 cut(s) 85, 325, 418, 552, 657, 1104, 1627
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 7 cut(s) 82, 322, 415, 549, 654, 1101, 1624
BstMWI GCNNNNNNNGC 3 cut(s) 1118, 1337, 1615
BstNI CCWGG 2 cut(s) 46, 1508
BstNSI RCATGY 3 cut(s) 1394, 1563, 1618
BstPAI GACNNNNGTC 1 cut(s) 120
BstSCI CCNGG 2 cut(s) 44, 1506
BstSFI CTRYAG 2 cut(s) 51, 1329
BstV1I GCAGC 2 cut(s) 736, 780
BstV2I GAAGAC 1 cut(s) 507
BstX2I RGATCY 1 cut(s) 1624
BstXI CCANNNNNNTGG 2 cut(s) 75, 1293
BstYI RGATCY 1 cut(s) 1624
BstZ17I GTATAC 1 cut(s) 1056
Bsu15I ATCGAT 2 cut(s) 653, 1002
Bsu36I CCTNAGG 1 cut(s) 1222
BsuI GTATCC 1 cut(s) 483
BsuRI GGCC 4 cut(s) 49, 290, 1301, 1531
BsuTUI ATCGAT 2 cut(s) 653, 1002
BtgI CCRYGG 1 cut(s) 1260
BtrI CACGTC 1 cut(s) 1051
BtsCI GGATG 3 cut(s) 784, 1198, 1576
BtsI GCAGTG 3 cut(s) 1007, 1232, 1532
BtsIMutI CAGTG 6 cut(s) 186, 1007, 1040, 1214, 1232, 1532
Cac8I GCNNGC 3 cut(s) 1077, 1134, 1616
CfoI GCGC 1 cut(s) 228
Cfr13I GGNCC 1 cut(s) 1510
ClaI ATCGAT 2 cut(s) 653, 1002
CseI GACGC 1 cut(s) 1487
Csp6I GTAC 3 cut(s) 469, 942, 1640
CspCI CAANNNNNGTGG 4 cut(s) 523, 558, 1234, 1269
CviAII CATG 7 cut(s) 1261, 1391, 1417, 1482, 1560, 1610, 1615
CviQI GTAC 3 cut(s) 469, 942, 1640
DdeI CTNAG 6 cut(s) 208, 356, 485, 819, 1222, 1605
DpnI GATC 7 cut(s) 84, 324, 417, 551, 656, 1103, 1626
DpnII GATC 7 cut(s) 82, 322, 415, 549, 654, 1101, 1624
EaeI YGGCCR 1 cut(s) 1299
Eam1104I CTCTTC 3 cut(s) 12, 441, 1065
EarI CTCTTC 3 cut(s) 12, 441, 1065
EciI GGCGGA 1 cut(s) 122
Ecl136II GAGCTC 1 cut(s) 961
Eco130I CCWWGG 2 cut(s) 229, 1260
Eco147I AGGCCT 1 cut(s) 49
Eco24I GRGCYC 1 cut(s) 963
Eco47I GGWCC 1 cut(s) 1510
Eco53kI GAGCTC 1 cut(s) 961
Eco57I CTGAAG 5 cut(s) 103, 177, 422, 990, 1080
Eco81I CCTNAGG 1 cut(s) 1222
Eco88I CYCGRG 1 cut(s) 509
EcoICRI GAGCTC 1 cut(s) 961
EcoNI CCTNNNNNAGG 1 cut(s) 874
EcoRI GAATTC 1 cut(s) 1545
EcoRII CCWGG 2 cut(s) 44, 1506
EcoT14I CCWWGG 2 cut(s) 229, 1260
EcoT38I GRGCYC 1 cut(s) 963
ErhI CCWWGG 2 cut(s) 229, 1260
FaeI CATG 7 cut(s) 1264, 1394, 1420, 1485, 1563, 1613, 1618
FalI AAGNNNNNCTT 2 cut(s) 1059, 1091
FaqI GGGAC 1 cut(s) 1496
FatI CATG 7 cut(s) 1260, 1390, 1416, 1481, 1559, 1609, 1614
FbaI TGATCA 1 cut(s) 415
FblI GTMKAC 2 cut(s) 825, 1055
Fnu4HI GCNGC 2 cut(s) 725, 794
FokI GGATG 3 cut(s) 791, 1205, 1583
FriOI GRGCYC 1 cut(s) 963
Fsp4HI GCNGC 2 cut(s) 725, 794
FspBI CTAG 5 cut(s) 230, 521, 876, 956, 1137
GlaI GCGC 1 cut(s) 227
GluI GCNGC 2 cut(s) 725, 794
GsaI CCCAGC 1 cut(s) 838
HaeII RGCGCY 1 cut(s) 229
HaeIII GGCC 4 cut(s) 49, 290, 1301, 1531
HgaI GACGC 1 cut(s) 1487
HhaI GCGC 1 cut(s) 228
Hin1II CATG 7 cut(s) 1264, 1394, 1420, 1485, 1563, 1613, 1618
Hin6I GCGC 1 cut(s) 226
HinP1I GCGC 1 cut(s) 226
HindIII AAGCTT 1 cut(s) 463
HinfI GANTC 5 cut(s) 545, 601, 671, 1499, 1540
HphI GGTGA 1 cut(s) 520
Hpy166II GTNNAC 6 cut(s) 115, 801, 826, 944, 1056, 1579
Hpy188I TCNGA 5 cut(s) 196, 420, 441, 1101, 1629
Hpy188III TCNNGA 7 cut(s) 272, 453, 487, 542, 691, 917, 1151
Hpy8I GTNNAC 6 cut(s) 115, 801, 826, 944, 1056, 1579
HpyAV CCTTC 5 cut(s) 201, 401, 763, 1315, 1480
HpyCH4III ACNGT 3 cut(s) 299, 560, 1044
HpyCH4IV ACGT 1 cut(s) 1050
HpyCH4V TGCA 8 cut(s) 724, 891, 1007, 1079, 1132, 1331, 1397, 1559
HpyF10VI GCNNNNNNNGC 3 cut(s) 1118, 1337, 1615
HpyF3I CTNAG 6 cut(s) 208, 356, 485, 819, 1222, 1605
HpySE526I ACGT 1 cut(s) 1050
Hsp92II CATG 7 cut(s) 1264, 1394, 1420, 1485, 1563, 1613, 1618
HspAI GCGC 1 cut(s) 226
Ksp22I TGATCA 1 cut(s) 415
Kzo9I GATC 7 cut(s) 82, 322, 415, 549, 654, 1101, 1624
LguI GCTCTTC 1 cut(s) 1065
LmnI GCTCC 1 cut(s) 1430
Lsp1109I GCAGC 2 cut(s) 736, 780
LweI GCATC 5 cut(s) 29, 156, 630, 994, 1406
MaeI CTAG 5 cut(s) 230, 521, 876, 956, 1137
MaeII ACGT 1 cut(s) 1050
MaeIII GTNAC 6 cut(s) 122, 814, 895, 1142, 1255, 1475
MalI GATC 7 cut(s) 84, 324, 417, 551, 656, 1103, 1626
MboI GATC 7 cut(s) 82, 322, 415, 549, 654, 1101, 1624
MboII GAAGA 9 cut(s) 428, 512, 962, 1079, 1082, 1277, 1451, 1454, 1601
MflI RGATCY 1 cut(s) 1624
MhlI GDGCHC 1 cut(s) 963
MlsI TGGCCA 1 cut(s) 1301
MluNI TGGCCA 1 cut(s) 1301
MlyI GAGTC 2 cut(s) 554, 1534
MmeI TCCRAC 1 cut(s) 687
MnlI CCTC 9 cut(s) 505, 508, 814, 873, 919, 975, 1080, 1231, 1379
Mox20I TGGCCA 1 cut(s) 1301
MscI TGGCCA 1 cut(s) 1301
MseI TTAA 4 cut(s) 426, 461, 563, 1551
MslI CAYNNNNRTG 3 cut(s) 300, 629, 666
Msp20I TGGCCA 1 cut(s) 1301
MspA1I CMGCKG 1 cut(s) 1403
MspR9I CCNGG 2 cut(s) 46, 1508
MvaI CCWGG 2 cut(s) 46, 1508
MwoI GCNNNNNNNGC 3 cut(s) 1118, 1337, 1615
NcoI CCATGG 1 cut(s) 1260
NdeII GATC 7 cut(s) 82, 322, 415, 549, 654, 1101, 1624
NlaIII CATG 7 cut(s) 1264, 1394, 1420, 1485, 1563, 1613, 1618
NlaIV GGNNCC 4 cut(s) 177, 1166, 1432, 1512
NmuCI GTSAC 2 cut(s) 122, 1475
NspI RCATGY 3 cut(s) 1394, 1563, 1618
NspV TTCGAA 1 cut(s) 613
OliI CACNNNNGTG 1 cut(s) 666
PaeI GCATGC 1 cut(s) 1618
PaeR7I CTCGAG 1 cut(s) 509
PceI AGGCCT 1 cut(s) 49
PciI ACATGT 1 cut(s) 1390
PciSI GCTCTTC 1 cut(s) 1065
PfeI GAWTC 3 cut(s) 601, 671, 1499
PkrI GCNGC 2 cut(s) 726, 795
PleI GAGTC 2 cut(s) 553, 1534
PpsI GAGTC 2 cut(s) 553, 1534
PscI ACATGT 1 cut(s) 1390
PshAI GACNNNNGTC 1 cut(s) 120
PsiI TTATAA 3 cut(s) 372, 1035, 1241
Psp124BI GAGCTC 1 cut(s) 963
Psp6I CCWGG 2 cut(s) 44, 1506
PspFI CCCAGC 1 cut(s) 834
PspGI CCWGG 2 cut(s) 44, 1506
PspN4I GGNNCC 4 cut(s) 177, 1166, 1432, 1512
PspPI GGNCC 1 cut(s) 1510
PspXI VCTCGAGB 1 cut(s) 509
PstI CTGCAG 1 cut(s) 1333
PsuI RGATCY 1 cut(s) 1624
PvuII CAGCTG 1 cut(s) 1403
RsaI GTAC 3 cut(s) 470, 943, 1641
RsaNI GTAC 3 cut(s) 469, 942, 1640
RseI CAYNNNNRTG 3 cut(s) 300, 629, 666
SacI GAGCTC 1 cut(s) 963
SapI GCTCTTC 1 cut(s) 1065
SaqAI TTAA 4 cut(s) 426, 461, 563, 1551
SatI GCNGC 2 cut(s) 725, 794
Sau3AI GATC 7 cut(s) 82, 322, 415, 549, 654, 1101, 1624
Sau96I GGNCC 1 cut(s) 1510
SchI GAGTC 2 cut(s) 554, 1534
ScrFI CCNGG 2 cut(s) 46, 1508
SduI GDGCHC 1 cut(s) 963
SfaNI GCATC 5 cut(s) 29, 156, 630, 994, 1406
SfcI CTRYAG 2 cut(s) 51, 1329
Sfr274I CTCGAG 1 cut(s) 509
SfuI TTCGAA 1 cut(s) 613
SinI GGWCC 1 cut(s) 1510
SlaI CTCGAG 1 cut(s) 509
SmiMI CAYNNNNRTG 3 cut(s) 300, 629, 666
SmlI CTYRAG 3 cut(s) 509, 542, 1619
SmoI CTYRAG 3 cut(s) 509, 542, 1619
SphI GCATGC 1 cut(s) 1618
SseBI AGGCCT 1 cut(s) 49
SsiI CCGC 1 cut(s) 107
SspMI CTAG 5 cut(s) 230, 521, 876, 956, 1137
SstI GAGCTC 1 cut(s) 963
StuI AGGCCT 1 cut(s) 49
StyD4I CCNGG 2 cut(s) 44, 1506
StyI CCWWGG 2 cut(s) 229, 1260
TaaI ACNGT 3 cut(s) 299, 560, 1044
TaiI ACGT 1 cut(s) 1053
TatI WGTACW 1 cut(s) 1639
TfiI GAWTC 3 cut(s) 601, 671, 1499
Tru1I TTAA 4 cut(s) 426, 461, 563, 1551
Tru9I TTAA 4 cut(s) 426, 461, 563, 1551
TscAI CASTG 6 cut(s) 186, 1014, 1047, 1221, 1239, 1539
TseFI GTSAC 2 cut(s) 122, 1475
TseI GCWGC 2 cut(s) 724, 793
Tsp45I GTSAC 2 cut(s) 122, 1475
TspDTI ATGAA 7 cut(s) 696, 722, 1037, 1139, 1209, 1433, 1602
TspGWI ACGGA 1 cut(s) 510
TspRI CASTG 6 cut(s) 186, 1014, 1047, 1221, 1239, 1539
VpaK11BI GGWCC 1 cut(s) 1510
XagI CCTNNNNNAGG 1 cut(s) 874
XapI RAATTY 3 cut(s) 279, 1424, 1545
XceI RCATGY 3 cut(s) 1394, 1563, 1618
XhoI CTCGAG 1 cut(s) 509
XmaJI CCTAGG 1 cut(s) 229
XmiI GTMKAC 2 cut(s) 825, 1055
XspI CTAG 5 cut(s) 230, 521, 876, 956, 1137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.