Prupe.4G199000_v2.0.a1

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000053
Physical Location & Seq
Forward (+)
12240240 .. 12242495
2256 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G199000.1

Sequence Viewer

Length: 1695 bp
ATGGCTCTAGAAGCTGGTTCAACTGCTCAATCACAAAATTCTAAGGCTGATCAAATTGTTCGACGGACAGCAAATTACCACCCGAGCATTTGGGGGGATCAGTTTATGAATTATGATTCAGTAGACATTATAACTTATGCCCATAAGCAACAAGAAGTTGAAGAATTGAAACTAGCAGTGAGGAGGGAAGTGTTCACAACCAGTGCAGATGATTTTTCAAATCAGATGAAGCTAATTGATGCAATCCAGCGCCTAGGCGTGGCGTACCACTTTGAAAAGGAAATAGAAGAAGCACTGGAACATATATATGCTGCAAATCATTTCCATGATGATGATGATGGTGATGGTGATCTATACAATGTTTCTCTTGGTTTTCGGCTACTAAGACAACATGGACACAATGTTTCATGCAGAATATTCAACAAGTTCAAAGATAGTAAAAATGGAGGCTTCAAGGAAAGCTTAATCGCTGATGTGTCGGGATTGCTAAGCTTTTATGAAGCTACGCATCTGAAGGTGCATGGAGAAGATATACTAGAAGAGGCTCTTGTTTTCACGACAAAACATCTGGAGTCGGCAACAACCCATGTAAGCTATCAACTAGCAGAACAAATAGCTCAAGCCCTAGAGAGACCGCTCCGAAAGAGTCTCGTGAGGTTATGCGCCCGTCGGTTCATGTCCATCTACCAAGATGAAGCTTCACATAATGAAGCTCTATTAAAACTTGCTAAGTCAGATTTCAATCTTGTTCAGTCTTTGCACAAACAGGAGCTCAGTGAGATTATTAGGTGGTGGAAAGAACTAGACTTTGGAAGGAAGCTGCCTTTTGCAAGAAACAGAATTGTGGAGTTATACTGCTGGATTTTGGGGGTCTATTTTGAGCCCCAATACCTTGTTGGAAGAAAATTTCTCACAAAAATTCTTGCCCTGATGTCTGTAATGGATGATATCTATGACGCATTCGGTACATTTGAAGAACTTGAGATCTTTACCGAAGCAATTCATCAAAGATGGCATGCCAATTGCATGGATGGACTGCCAGACTACATGCAAACATTCTTCCATGCGCTTTTGAATGTTTTCAATGAAATTGAGGAGGAGATGGTGAAGGAAGGAAGAGCATACCGAGCTCACTATGCAAAAGAAGCGTGGAAAACTATTACCAAAGCTTACTTTGATGAGGCCAAATGGTTCCACGAAGGATGCATCCCAAGCATGGAGGAGTATATGCGTGTTGCTGCAACTTCTGCTGCTAGCTTCGCACTTTCAACTACATCTTTAGTTGGCATGGGAGATATTGTAACCAAGGAGTCCTTTGAGTGGTTGTTCAATGACCCTAAAATTCTTAAAGCTTCCAATATCATTGTTAGGCTCATGGATGACATCGTTTCAAGCAAGTTTGAAAAAGAGAGGGGCCATGTTGCTTGTGCTATTGATTGTTACATGAAGCAATATGGGGTCTCAGATGAGCAAGAGATAATTGATGTCTTCAACAAACAAATTGTGGATTCGTGGAAGGATATCAACGAGGAGTTCCTTAGACCAACTTCTATGCCGATGCCTATCCTTGAACGTATTGTTAATTTAACAAGAGTGGTGGATCTCCTCTACAAAAAATATGATGCATACACACATGGTGGAAAAGTGATGAACGATTGCATCGCTTCGTATTTGATTGATCTGGCACCAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

565

Amino Acids

65.17

Weight (kDa)

5.36

Isoelectric Point (pI)

47.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 131
AccB1I GGYRCC 1 cut(s) 1684
AccBSI CCGCTC 1 cut(s) 637
AccI GTMKAC 1 cut(s) 123
AciI CCGC 1 cut(s) 635
AclWI GGATC 2 cut(s) 105, 1608
AcsI RAATTY 4 cut(s) 37, 905, 918, 1341
AcuI CTGAAG 1 cut(s) 533
AdeI CACNNNGTG 1 cut(s) 1637
AfaI GTAC 2 cut(s) 266, 967
AfiI CCNNNNNNNGG 3 cut(s) 259, 1216, 1320
AjuI GAANNNNNNNTTGG 2 cut(s) 792, 824
Alw21I GWGCWC 2 cut(s) 774, 1132
Alw26I GTCTC 3 cut(s) 625, 653, 1465
AlwI GGATC 2 cut(s) 105, 1608
Ama87I CYCGRG 1 cut(s) 82
AoxI GGCC 2 cut(s) 1182, 1414
ApeKI GCWGC 4 cut(s) 311, 820, 1238, 1250
ApoI RAATTY 4 cut(s) 37, 905, 918, 1341
Asp700I GAANNNNTTC 2 cut(s) 999, 1079
AspA2I CCTAGG 1 cut(s) 253
AspLEI GCGC 3 cut(s) 252, 665, 1069
AspS9I GGNCC 1 cut(s) 1414
AsuHPI GGTGA 3 cut(s) 353, 359, 1117
AsuNHI GCTAGC 1 cut(s) 1253
AvaI CYCGRG 1 cut(s) 82
AvrII CCTAGG 1 cut(s) 253
BanI GGYRCC 1 cut(s) 1684
BanII GRGCYC 3 cut(s) 774, 885, 1132
BauI CACGAG 1 cut(s) 650
BbsI GAAGAC 1 cut(s) 1480
Bbv12I GWGCWC 2 cut(s) 774, 1132
BbvI GCAGC 4 cut(s) 298, 807, 1225, 1237
BccI CCATC 6 cut(s) 332, 338, 689, 1005, 1025, 1096
BclI TGATCA 1 cut(s) 49
BcoDI GTCTC 3 cut(s) 625, 653, 1465
BfaI CTAG 8 cut(s) 8, 173, 254, 536, 602, 626, 803, 1254
BfoI RGCGCY 1 cut(s) 253
BglII AGATCT 1 cut(s) 984
BisI GCNGC 4 cut(s) 312, 821, 1239, 1251
BlnI CCTAGG 1 cut(s) 253
BlpI GCTNAGC 1 cut(s) 488
BlsI GCNGC 4 cut(s) 313, 822, 1240, 1252
BmeT110I CYCGRG 1 cut(s) 82
BmgT120I GGNCC 1 cut(s) 1414
BmiI GGNNCC 3 cut(s) 1193, 1415, 1686
BmsI GCATC 7 cut(s) 229, 517, 1193, 1215, 1548, 1612, 1668
BmtI GCTAGC 1 cut(s) 1257
BpiI GAAGAC 1 cut(s) 1480
BpmI CTGGAG 1 cut(s) 590
Bpu1102I GCTNAGC 1 cut(s) 488
BpuEI CTTGAG 2 cut(s) 603, 1001
BsaBI GATNNNNATC 3 cut(s) 348, 741, 1562
BsaI GGTCTC 2 cut(s) 625, 1465
BsaJI CCNNGG 2 cut(s) 253, 1305
BsaXI ACNNNNNCTCC 2 cut(s) 1284, 1314
Bsc4I CCNNNNNNNGG 3 cut(s) 259, 1216, 1320
Bse1I ACTGG 3 cut(s) 201, 300, 1688
Bse8I GATNNNNATC 3 cut(s) 348, 741, 1562
BseDI CCNNGG 2 cut(s) 253, 1305
BseGI GGATG 5 cut(s) 949, 1036, 1206, 1208, 1384
BseJI GATNNNNATC 3 cut(s) 348, 741, 1562
BseLI CCNNNNNNNGG 3 cut(s) 259, 1216, 1320
BseMII CTCAG 2 cut(s) 787, 1476
BseNI ACTGG 3 cut(s) 201, 300, 1688
BseRI GAGGAG 6 cut(s) 196, 1109, 1112, 1235, 1544, 1595
BseXI GCAGC 4 cut(s) 298, 807, 1225, 1237
BsgI GTGCAG 1 cut(s) 225
BshFI GGCC 2 cut(s) 1184, 1416
BshNI GGYRCC 1 cut(s) 1684
BsiHKAI GWGCWC 2 cut(s) 774, 1132
BsiHKCI CYCGRG 1 cut(s) 82
BslI CCNNNNNNNGG 3 cut(s) 259, 1216, 1320
BsmAI GTCTC 3 cut(s) 625, 653, 1465
BsmI GAATGC 1 cut(s) 959
BsnI GGCC 2 cut(s) 1184, 1416
Bso31I GGTCTC 2 cut(s) 625, 1465
BsoBI CYCGRG 1 cut(s) 82
Bsp1286I GDGCHC 3 cut(s) 774, 885, 1132
Bsp143I GATC 6 cut(s) 49, 97, 349, 984, 1600, 1678
Bsp1720I GCTNAGC 1 cut(s) 488
BspACI CCGC 1 cut(s) 635
BspANI GGCC 2 cut(s) 1184, 1416
BspCNI CTCAG 2 cut(s) 786, 1475
BspLI GGNNCC 3 cut(s) 1193, 1415, 1686
BspOI GCTAGC 1 cut(s) 1257
BspPI GGATC 2 cut(s) 105, 1608
BspQI GCTCTTC 1 cut(s) 1111
BspT107I GGYRCC 1 cut(s) 1684
BspTNI GGTCTC 2 cut(s) 625, 1465
BsrBI CCGCTC 1 cut(s) 637
BsrI ACTGG 3 cut(s) 201, 300, 1688
BssECI CCNNGG 2 cut(s) 253, 1305
BssMI GATC 6 cut(s) 49, 97, 349, 984, 1600, 1678
BssSI CACGAG 1 cut(s) 650
BssT1I CCWWGG 2 cut(s) 253, 1305
Bst2BI CACGAG 1 cut(s) 650
Bst6I CTCTTC 2 cut(s) 534, 1111
BstAPI GCANNNNNTGC 1 cut(s) 1247
BstC8I GCNNGC 2 cut(s) 1017, 1255
BstDEI CTNAG 7 cut(s) 42, 383, 488, 729, 773, 1462, 1538
BstF5I GGATG 5 cut(s) 949, 1036, 1206, 1208, 1384
BstH2I RGCGCY 1 cut(s) 253
BstHHI GCGC 3 cut(s) 252, 665, 1069
BstKTI GATC 6 cut(s) 52, 100, 352, 987, 1603, 1681
BstMAI GTCTC 3 cut(s) 625, 653, 1465
BstMBI GATC 6 cut(s) 49, 97, 349, 984, 1600, 1678
BstMWI GCNNNNNNNGC 7 cut(s) 11, 1127, 1136, 1145, 1212, 1247, 1259
BstNSI RCATGY 2 cut(s) 1019, 1051
BstV1I GCAGC 4 cut(s) 298, 807, 1225, 1237
BstV2I GAAGAC 1 cut(s) 1480
BstX2I RGATCY 2 cut(s) 984, 1600
BstXI CCANNNNNNTGG 1 cut(s) 1027
BstYI RGATCY 2 cut(s) 984, 1600
BsuRI GGCC 2 cut(s) 1184, 1416
BtgZI GCGATG 1 cut(s) 1645
BtsCI GGATG 5 cut(s) 949, 1036, 1206, 1208, 1384
BtsI GCAGTG 1 cut(s) 183
BtsIMutI CAGTG 4 cut(s) 183, 208, 293, 781
Cac8I GCNNGC 2 cut(s) 1017, 1255
CfoI GCGC 3 cut(s) 252, 665, 1069
Cfr13I GGNCC 1 cut(s) 1414
CseI GACGC 1 cut(s) 965
Csp6I GTAC 2 cut(s) 265, 966
CspCI CAANNNNNGTGG 2 cut(s) 1578, 1613
CviQI GTAC 2 cut(s) 265, 966
DdeI CTNAG 7 cut(s) 42, 383, 488, 729, 773, 1462, 1538
DpnI GATC 6 cut(s) 51, 99, 351, 986, 1602, 1680
DpnII GATC 6 cut(s) 49, 97, 349, 984, 1600, 1678
DraIII CACNNNGTG 1 cut(s) 1637
Eam1104I CTCTTC 2 cut(s) 534, 1111
EarI CTCTTC 2 cut(s) 534, 1111
Ecl136II GAGCTC 2 cut(s) 772, 1130
Eco130I CCWWGG 2 cut(s) 253, 1305
Eco24I GRGCYC 3 cut(s) 774, 885, 1132
Eco31I GGTCTC 2 cut(s) 625, 1465
Eco32I GATATC 2 cut(s) 949, 1522
Eco53kI GAGCTC 2 cut(s) 772, 1130
Eco57I CTGAAG 1 cut(s) 533
Eco88I CYCGRG 1 cut(s) 82
EcoICRI GAGCTC 2 cut(s) 772, 1130
EcoRV GATATC 2 cut(s) 949, 1522
EcoT14I CCWWGG 2 cut(s) 253, 1305
EcoT22I ATGCAT 2 cut(s) 1208, 1627
EcoT38I GRGCYC 3 cut(s) 774, 885, 1132
ErhI CCWWGG 2 cut(s) 253, 1305
FalI AAGNNNNNCTT 6 cut(s) 446, 478, 531, 563, 1298, 1330
FbaI TGATCA 1 cut(s) 49
FblI GTMKAC 1 cut(s) 123
Fnu4HI GCNGC 4 cut(s) 312, 821, 1239, 1251
FokI GGATG 5 cut(s) 956, 1043, 1193, 1215, 1391
FriOI GRGCYC 3 cut(s) 774, 885, 1132
Fsp4HI GCNGC 4 cut(s) 312, 821, 1239, 1251
FspBI CTAG 8 cut(s) 8, 173, 254, 536, 602, 626, 803, 1254
GlaI GCGC 3 cut(s) 251, 664, 1068
GluI GCNGC 4 cut(s) 312, 821, 1239, 1251
GsuI CTGGAG 1 cut(s) 590
HaeII RGCGCY 1 cut(s) 253
HaeIII GGCC 2 cut(s) 1184, 1416
HgaI GACGC 1 cut(s) 965
HhaI GCGC 3 cut(s) 252, 665, 1069
Hin6I GCGC 3 cut(s) 250, 663, 1067
HinP1I GCGC 3 cut(s) 250, 663, 1067
HindIII AAGCTT 5 cut(s) 460, 490, 696, 1167, 1350
HinfI GANTC 5 cut(s) 116, 572, 646, 1310, 1508
HphI GGTGA 3 cut(s) 353, 359, 1117
Hpy166II GTNNAC 2 cut(s) 124, 195
Hpy188I TCNGA 5 cut(s) 225, 513, 641, 736, 1465
Hpy188III TCNNGA 5 cut(s) 8, 480, 556, 569, 652
Hpy8I GTNNAC 2 cut(s) 124, 195
Hpy99I CGWCG 2 cut(s) 66, 672
HpyAV CCTTC 6 cut(s) 508, 807, 1102, 1106, 1193, 1510
HpyCH4IV ACGT 1 cut(s) 1573
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 1127, 1136, 1145, 1212, 1247, 1259
HpyF3I CTNAG 7 cut(s) 42, 383, 488, 729, 773, 1462, 1538
HpySE526I ACGT 1 cut(s) 1573
HspAI GCGC 3 cut(s) 250, 663, 1067
Ksp22I TGATCA 1 cut(s) 49
Kzo9I GATC 6 cut(s) 49, 97, 349, 984, 1600, 1678
LguI GCTCTTC 1 cut(s) 1111
LmnI GCTCC 2 cut(s) 642, 769
LpnPI CCDG 9 cut(s) 214, 260, 281, 554, 752, 844, 941, 1053, 1667
Lsp1109I GCAGC 4 cut(s) 298, 807, 1225, 1237
LweI GCATC 7 cut(s) 229, 517, 1193, 1215, 1548, 1612, 1668
MaeI CTAG 8 cut(s) 8, 173, 254, 536, 602, 626, 803, 1254
MaeII ACGT 1 cut(s) 1573
MaeIII GTNAC 2 cut(s) 1300, 1439
MalI GATC 6 cut(s) 51, 99, 351, 986, 1602, 1680
MbiI CCGCTC 1 cut(s) 637
MboI GATC 6 cut(s) 49, 97, 349, 984, 1600, 1678
MboII GAAGA 9 cut(s) 173, 299, 539, 551, 912, 986, 1051, 1128, 1480
MfeI CAATTG 1 cut(s) 1021
MflI RGATCY 2 cut(s) 984, 1600
MhlI GDGCHC 3 cut(s) 774, 885, 1132
MlyI GAGTC 3 cut(s) 581, 655, 1319
MmeI TCCRAC 1 cut(s) 877
Mph1103I ATGCAT 2 cut(s) 1208, 1627
MroXI GAANNNNTTC 2 cut(s) 999, 1079
MseI TTAA 5 cut(s) 464, 719, 1347, 1581, 1586
MslI CAYNNNNRTG 4 cut(s) 306, 324, 330, 1690
MunI CAATTG 1 cut(s) 1021
Mva1269I GAATGC 1 cut(s) 959
MwoI GCNNNNNNNGC 7 cut(s) 11, 1127, 1136, 1145, 1212, 1247, 1259
NdeII GATC 6 cut(s) 49, 97, 349, 984, 1600, 1678
NheI GCTAGC 1 cut(s) 1253
NlaIV GGNNCC 3 cut(s) 1193, 1415, 1686
NsiI ATGCAT 2 cut(s) 1208, 1627
NspI RCATGY 2 cut(s) 1019, 1051
PaeI GCATGC 1 cut(s) 1019
PciSI GCTCTTC 1 cut(s) 1111
PctI GAATGC 1 cut(s) 959
PdmI GAANNNNTTC 2 cut(s) 999, 1079
PfeI GAWTC 2 cut(s) 116, 1508
PkrI GCNGC 4 cut(s) 313, 822, 1240, 1252
PleI GAGTC 3 cut(s) 580, 654, 1318
PpsI GAGTC 3 cut(s) 580, 654, 1318
PsiI TTATAA 1 cut(s) 131
Psp124BI GAGCTC 2 cut(s) 774, 1132
PspN4I GGNNCC 3 cut(s) 1193, 1415, 1686
PspPI GGNCC 1 cut(s) 1414
PsuI RGATCY 2 cut(s) 984, 1600
RsaI GTAC 2 cut(s) 266, 967
RsaNI GTAC 2 cut(s) 265, 966
RseI CAYNNNNRTG 4 cut(s) 306, 324, 330, 1690
SacI GAGCTC 2 cut(s) 774, 1132
SapI GCTCTTC 1 cut(s) 1111
SaqAI TTAA 5 cut(s) 464, 719, 1347, 1581, 1586
SatI GCNGC 4 cut(s) 312, 821, 1239, 1251
Sau3AI GATC 6 cut(s) 49, 97, 349, 984, 1600, 1678
Sau96I GGNCC 1 cut(s) 1414
SchI GAGTC 3 cut(s) 581, 655, 1319
SduI GDGCHC 3 cut(s) 774, 885, 1132
SfaNI GCATC 7 cut(s) 229, 517, 1193, 1215, 1548, 1612, 1668
SmiMI CAYNNNNRTG 4 cut(s) 306, 324, 330, 1690
SmlI CTYRAG 2 cut(s) 618, 980
SmoI CTYRAG 2 cut(s) 618, 980
SphI GCATGC 1 cut(s) 1019
SsiI CCGC 1 cut(s) 635
SspI AATATT 1 cut(s) 417
SspMI CTAG 8 cut(s) 8, 173, 254, 536, 602, 626, 803, 1254
SstI GAGCTC 2 cut(s) 774, 1132
StyI CCWWGG 2 cut(s) 253, 1305
TaiI ACGT 1 cut(s) 1576
TaqI TCGA 1 cut(s) 61
TfiI GAWTC 2 cut(s) 116, 1508
Tru1I TTAA 5 cut(s) 464, 719, 1347, 1581, 1586
Tru9I TTAA 5 cut(s) 464, 719, 1347, 1581, 1586
TscAI CASTG 4 cut(s) 183, 208, 300, 781
TseI GCWGC 4 cut(s) 311, 820, 1238, 1250
TspGWI ACGGA 1 cut(s) 79
TspRI CASTG 4 cut(s) 183, 208, 300, 781
XapI RAATTY 4 cut(s) 37, 905, 918, 1341
XbaI TCTAGA 1 cut(s) 7
XceI RCATGY 2 cut(s) 1019, 1051
XcmI CCANNNNNNNNNTGG 1 cut(s) 893
XmaJI CCTAGG 1 cut(s) 253
XmiI GTMKAC 1 cut(s) 123
XmnI GAANNNNTTC 2 cut(s) 999, 1079
XspI CTAG 8 cut(s) 8, 173, 254, 536, 602, 626, 803, 1254
Zsp2I ATGCAT 2 cut(s) 1208, 1627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.