MD03G1214900.v1.1

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000053
Physical Location & Seq
Forward (+)
29537010 .. 29541297
4288 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1214900.v1.1.491

Sequence Viewer

Length: 1683 bp
ATGCCTATCCCAATTTGTGCAGGAGTCCTTTCGACAAATGCTAAGCCCGAAATTGTTCGTCCGACGGCAAATTTCCCACCAAGCATTTGGGGAGATCGGTTCATCAACTATGATTCCCAAGACATTACAACTCATGCCCGTAAGAAACAAGAAATCGACGAGCTGAAAGAAGTAGTGAGGAGTGAAGTATTCAAAATTATGGCAAGTGATTTTTCACATCAGCTGAAGTTGATCGATGCAATCCAACGCCTTGGCGTGGCGTACCATTTCGCAAGTGAAATCGAAGAAACACTGGAACGTATGCATGGCACGTTTAATGACCATGAAGATGTTGGTGGTGATCTTCACATTGTTGCTCTTGGTTTTCGGCTACTAAGACAACACGGATATAATGTTTCATGTGATATATTCAACAAGTTCAAAGATGCAAATGGCAAATTCATGGAATGCTTAACTGCCGACATTCCGGGCATGCTAAGCCTTTATGAAGCAGCACATCTAGGGGTCCGGGGTGAAGATATACTTGATGAGGCTCTTGTTTTCACCACCACTCACCTTGAGTCAGCTTCAACAACCAATGTAAGCTATCAGCTGGCAGAACAAACAACTCAAGCCTTAGAGCGACCTTTGCAAAAGGATCTAGAGAGGGTATGTGCCAGGCGTCACATGTCAATCTACCAAGATGAAGCTGCACATAACGAAGCTCTACTGAAACTTGCAAAGTTAGATTTCAATCTGGTTCAATCTTTACACAAAAAGGAGCTTAGTGAGATTACTAGGTGGTGGAAAGAACTAGACTTTGAAAAGAAGCTGCCTTTTGCAAGAGATAGGATTGTGGAGTTGTACCTTTGGATAGTGGGAGTATATTTTGAACCCCAATACGATAGAGCAAGAAAGTTTCTTACAAAAATTATTGCCCTGGTATCAGTGATGGATGATATCTACGACGCATATGCTACATTCAAAGAACTCGAGATCTTTACCGGGGCAATTGAGAGGTGGGATATGAATTGCTTGGATGAACTCCCGGACTATATGCAAATATTTTATCATACACTTTTGAGTGTTATCAATGAAATTGAGGAAGAGATGGTCAAGGAAGGAAGATCATACCGAGTTTACTATACAAAGGAATCCTTGAAATGTGTTGCTCGAGCGTACTTTGAAGAGGCTAAATGGTTAAACGAAGGATACACCCCAACTATGGAGGAGTATTTGGCTGTTGCTATTGTGTCTACTGGCTACCCAATGCTTTCAACCGTATCCTTGGTTGGTATGGGAGATATCGTAACCAAGGAGACATTTGAGTGGCTCTTCAATGACGCCAAAATTGTTAGAGCTTCAACAACCCTTTTCAGGTTCATAGATGATATGGTTACGACCAAGTTTGAGCAAGAGAGAGGGCATGTTGCTTCTAGTGTTGATTGCTACATGAAGCAATATGGGGTGTCGGAGCAAGAGACGATCAAGGTTTTTAACAAACAAGTCGTGAATCTGTGGAAGGATATAAACGAGGAGTTCCACAGATCAACTGCAGTGCCAATGCCCGTCCTCATGCGTGTTCTCAATTTAACAAGGGTAGTAGATCTCCTCTACAAAGGTGACGATGGCTACACTCGTGTTGGTAAAGTGGTGAAGGATATTATTGCTGCACTTTTTATTGATCCAGTGCCAATTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

561

Amino Acids

64.59

Weight (kDa)

5.19

Isoelectric Point (pI)

40.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 29 - 206 9e-59 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 263 - 502 5.8e-100 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 303 - 502 4.2e-57 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1235
AclWI GGATC 2 cut(s) 645, 1658
AcsI RAATTY 2 cut(s) 70, 437
AcuI CTGAAG 1 cut(s) 245
AcyI GRCGYC 2 cut(s) 661, 1323
AfaI GTAC 3 cut(s) 263, 845, 1160
AfiI CCNNNNNNNGG 3 cut(s) 256, 1204, 1356
AflIII ACRYGT 1 cut(s) 666
AjnI CCWGG 2 cut(s) 656, 918
Alw26I GTCTC 2 cut(s) 1292, 1454
AlwI GGATC 2 cut(s) 645, 1658
Ama87I CYCGRG 2 cut(s) 971, 1152
ApeKI GCWGC 4 cut(s) 491, 689, 811, 1649
ApoI RAATTY 2 cut(s) 70, 437
Asp700I GAANNNNTTC 1 cut(s) 54
AspS9I GGNCC 1 cut(s) 505
AsuC2I CCSGG 4 cut(s) 468, 509, 985, 1028
AsuHPI GGTGA 6 cut(s) 350, 524, 535, 545, 1613, 1645
AvaI CYCGRG 2 cut(s) 971, 1152
AvaII GGWCC 1 cut(s) 505
BauI CACGAG 1 cut(s) 1617
BbvI GCAGC 4 cut(s) 503, 676, 798, 1636
BccI CCATC 3 cut(s) 925, 1084, 1601
BceAI ACGGC 1 cut(s) 81
BcgI CGANNNNNNTGC 2 cut(s) 935, 969
BciT130I CCWGG 2 cut(s) 658, 920
BciVI GTATCC 2 cut(s) 1184, 1273
BcnI CCSGG 4 cut(s) 468, 509, 985, 1028
BcoDI GTCTC 2 cut(s) 1292, 1454
BfaI CTAG 5 cut(s) 500, 641, 777, 794, 1416
BfmI CTRYAG 1 cut(s) 1533
BfuI GTATCC 2 cut(s) 1184, 1273
BglII AGATCT 2 cut(s) 975, 1585
BisI GCNGC 4 cut(s) 492, 690, 812, 1650
BlpI GCTNAGC 2 cut(s) 42, 476
BlsI GCNGC 4 cut(s) 493, 691, 813, 1651
Bme1390I CCNGG 6 cut(s) 468, 509, 658, 920, 985, 1028
Bme18I GGWCC 1 cut(s) 505
BmeT110I CYCGRG 2 cut(s) 971, 1152
BmgT120I GGNCC 1 cut(s) 505
BmiI GGNNCC 1 cut(s) 506
BmrFI CCNGG 6 cut(s) 468, 509, 658, 920, 985, 1028
BmsI GCATC 2 cut(s) 226, 415
Bpu1102I GCTNAGC 2 cut(s) 42, 476
BpuEI CTTGAG 2 cut(s) 578, 594
BpuMI CCSGG 4 cut(s) 468, 509, 985, 1028
Bsa29I ATCGAT 1 cut(s) 234
BsaBI GATNNNNATC 1 cut(s) 732
BsaHI GRCGYC 2 cut(s) 661, 1323
BsaJI CCNNGG 6 cut(s) 250, 508, 918, 984, 1266, 1293
BsaXI ACNNNNNCTCC 2 cut(s) 1272, 1302
Bsc4I CCNNNNNNNGG 3 cut(s) 256, 1204, 1356
Bse1I ACTGG 3 cut(s) 297, 1243, 1667
Bse8I GATNNNNATC 1 cut(s) 732
BseBI CCWGG 2 cut(s) 658, 920
BseCI ATCGAT 1 cut(s) 234
BseDI CCNNGG 6 cut(s) 250, 508, 918, 984, 1266, 1293
BseGI GGATG 2 cut(s) 940, 1024
BseJI GATNNNNATC 1 cut(s) 732
BseLI CCNNNNNNNGG 3 cut(s) 256, 1204, 1356
BseNI ACTGG 3 cut(s) 297, 1243, 1667
BseRI GAGGAG 4 cut(s) 193, 1223, 1529, 1580
BseXI GCAGC 4 cut(s) 503, 676, 798, 1636
BsgI GTGCAG 3 cut(s) 39, 675, 1635
BshVI ATCGAT 1 cut(s) 234
BsiHKCI CYCGRG 2 cut(s) 971, 1152
BsiSI CCGG 4 cut(s) 467, 508, 984, 1028
BslI CCNNNNNNNGG 3 cut(s) 256, 1204, 1356
BsmAI GTCTC 2 cut(s) 1292, 1454
BsmBI CGTCTC 1 cut(s) 1454
BsmI GAATGC 1 cut(s) 452
BsoBI CYCGRG 2 cut(s) 971, 1152
Bsp1720I GCTNAGC 2 cut(s) 42, 476
BspDI ATCGAT 1 cut(s) 234
BspLI GGNNCC 1 cut(s) 506
BspMAI CTGCAG 1 cut(s) 1537
BspPI GGATC 2 cut(s) 645, 1658
BspQI GCTCTTC 1 cut(s) 1319
BsrI ACTGG 3 cut(s) 297, 1243, 1667
BssECI CCNNGG 6 cut(s) 250, 508, 918, 984, 1266, 1293
BssNI GRCGYC 2 cut(s) 661, 1323
BssSI CACGAG 1 cut(s) 1617
BssT1I CCWWGG 3 cut(s) 250, 1266, 1293
Bst2BI CACGAG 1 cut(s) 1617
Bst2UI CCWGG 2 cut(s) 658, 920
Bst4CI ACNGT 1 cut(s) 1261
Bst6I CTCTTC 3 cut(s) 1080, 1161, 1319
BstACI GRCGYC 2 cut(s) 661, 1323
BstC8I GCNNGC 2 cut(s) 473, 594
BstDEI CTNAG 5 cut(s) 42, 374, 476, 616, 764
BstF5I GGATG 2 cut(s) 940, 1024
BstMAI GTCTC 2 cut(s) 1292, 1454
BstMWI GCNNNNNNNGC 2 cut(s) 477, 628
BstNI CCWGG 2 cut(s) 658, 920
BstNSI RCATGY 3 cut(s) 475, 670, 1409
BstSCI CCNGG 6 cut(s) 466, 507, 656, 918, 983, 1026
BstSFI CTRYAG 1 cut(s) 1533
BstV1I GCAGC 4 cut(s) 503, 676, 798, 1636
BstX2I RGATCY 3 cut(s) 637, 975, 1585
BstXI CCANNNNNNTGG 2 cut(s) 87, 251
BstYI RGATCY 3 cut(s) 637, 975, 1585
Bsu15I ATCGAT 1 cut(s) 234
BsuI GTATCC 2 cut(s) 1184, 1273
BsuTUI ATCGAT 1 cut(s) 234
BtsCI GGATG 2 cut(s) 940, 1024
BtsI GCAGTG 1 cut(s) 1542
BtsIMutI CAGTG 4 cut(s) 290, 933, 1542, 1674
Cac8I GCNNGC 2 cut(s) 473, 594
Cfr13I GGNCC 1 cut(s) 505
ClaI ATCGAT 1 cut(s) 234
CseI GACGC 3 cut(s) 650, 956, 1331
Csp6I GTAC 3 cut(s) 262, 844, 1159
CspCI CAANNNNNGTGG 2 cut(s) 538, 573
CviQI GTAC 3 cut(s) 262, 844, 1159
DdeI CTNAG 5 cut(s) 42, 374, 476, 616, 764
Eam1104I CTCTTC 3 cut(s) 1080, 1161, 1319
EarI CTCTTC 3 cut(s) 1080, 1161, 1319
Eco130I CCWWGG 3 cut(s) 250, 1266, 1293
Eco32I GATATC 2 cut(s) 940, 1285
Eco47I GGWCC 1 cut(s) 505
Eco57I CTGAAG 1 cut(s) 245
Eco88I CYCGRG 2 cut(s) 971, 1152
EcoRII CCWGG 2 cut(s) 656, 918
EcoRV GATATC 2 cut(s) 940, 1285
EcoT14I CCWWGG 3 cut(s) 250, 1266, 1293
EcoT22I ATGCAT 1 cut(s) 306
ErhI CCWWGG 3 cut(s) 250, 1266, 1293
Esp3I CGTCTC 1 cut(s) 1454
FalI AAGNNNNNCTT 4 cut(s) 507, 539, 1121, 1153
FauNDI CATATG 1 cut(s) 952
FblI GTMKAC 1 cut(s) 1235
Fnu4HI GCNGC 4 cut(s) 492, 690, 812, 1650
FokI GGATG 2 cut(s) 947, 1031
Fsp4HI GCNGC 4 cut(s) 492, 690, 812, 1650
FspBI CTAG 5 cut(s) 500, 641, 777, 794, 1416
GluI GCNGC 4 cut(s) 492, 690, 812, 1650
HapII CCGG 4 cut(s) 467, 508, 984, 1028
HgaI GACGC 3 cut(s) 650, 956, 1331
Hin1I GRCGYC 2 cut(s) 661, 1323
HinfI GANTC 5 cut(s) 24, 113, 560, 1133, 1492
HpaII CCGG 4 cut(s) 467, 508, 984, 1028
HphI GGTGA 6 cut(s) 350, 524, 535, 545, 1613, 1645
Hpy166II GTNNAC 2 cut(s) 1120, 1236
Hpy188I TCNGA 2 cut(s) 63, 1453
Hpy188III TCNNGA 3 cut(s) 641, 973, 1489
Hpy8I GTNNAC 2 cut(s) 1120, 1236
Hpy99I CGWCG 3 cut(s) 67, 161, 950
HpyAV CCTTC 4 cut(s) 1094, 1181, 1495, 1630
HpyCH4III ACNGT 1 cut(s) 1261
HpyCH4IV ACGT 2 cut(s) 298, 311
HpyF10VI GCNNNNNNNGC 2 cut(s) 477, 628
HpyF3I CTNAG 5 cut(s) 42, 374, 476, 616, 764
HpySE526I ACGT 2 cut(s) 298, 311
Hsp92I GRCGYC 2 cut(s) 661, 1323
LguI GCTCTTC 1 cut(s) 1319
LmnI GCTCC 2 cut(s) 760, 1453
Lsp1109I GCAGC 4 cut(s) 503, 676, 798, 1636
LweI GCATC 2 cut(s) 226, 415
MaeI CTAG 5 cut(s) 500, 641, 777, 794, 1416
MaeII ACGT 2 cut(s) 298, 311
MaeIII GTNAC 4 cut(s) 662, 1288, 1375, 1601
MboII GAAGA 8 cut(s) 296, 335, 338, 527, 1097, 1116, 1178, 1306
MfeI CAATTG 1 cut(s) 990
MflI RGATCY 3 cut(s) 637, 975, 1585
MlyI GAGTC 2 cut(s) 33, 569
MmeI TCCRAC 3 cut(s) 86, 268, 1431
Mph1103I ATGCAT 1 cut(s) 306
MroXI GAANNNNTTC 1 cut(s) 54
MseI TTAA 5 cut(s) 315, 452, 1181, 1476, 1571
MslI CAYNNNNRTG 2 cut(s) 327, 1306
MspA1I CMGCKG 2 cut(s) 223, 592
MspI CCGG 4 cut(s) 467, 508, 984, 1028
MspR9I CCNGG 6 cut(s) 468, 509, 658, 920, 985, 1028
MunI CAATTG 1 cut(s) 990
Mva1269I GAATGC 1 cut(s) 452
MvaI CCWGG 2 cut(s) 658, 920
MwoI GCNNNNNNNGC 2 cut(s) 477, 628
NciI CCSGG 4 cut(s) 468, 509, 985, 1028
NdeI CATATG 1 cut(s) 952
NlaIV GGNNCC 1 cut(s) 506
NmuCI GTSAC 2 cut(s) 662, 1601
NsiI ATGCAT 1 cut(s) 306
NspI RCATGY 3 cut(s) 475, 670, 1409
PaeI GCATGC 1 cut(s) 475
PaeR7I CTCGAG 2 cut(s) 971, 1152
PciI ACATGT 1 cut(s) 666
PciSI GCTCTTC 1 cut(s) 1319
PctI GAATGC 1 cut(s) 452
PdmI GAANNNNTTC 1 cut(s) 54
PfeI GAWTC 3 cut(s) 113, 1133, 1492
PfoI TCCNGGA 1 cut(s) 1026
PkrI GCNGC 4 cut(s) 493, 691, 813, 1651
PleI GAGTC 2 cut(s) 32, 568
PpsI GAGTC 2 cut(s) 32, 568
PscI ACATGT 1 cut(s) 666
Psp6I CCWGG 2 cut(s) 656, 918
PspGI CCWGG 2 cut(s) 656, 918
PspN4I GGNNCC 1 cut(s) 506
PspPI GGNCC 1 cut(s) 505
PspXI VCTCGAGB 1 cut(s) 1152
PstI CTGCAG 1 cut(s) 1537
PsuI RGATCY 3 cut(s) 637, 975, 1585
PvuII CAGCTG 2 cut(s) 223, 592
RsaI GTAC 3 cut(s) 263, 845, 1160
RsaNI GTAC 3 cut(s) 262, 844, 1159
RseI CAYNNNNRTG 2 cut(s) 327, 1306
SapI GCTCTTC 1 cut(s) 1319
SaqAI TTAA 5 cut(s) 315, 452, 1181, 1476, 1571
SatI GCNGC 4 cut(s) 492, 690, 812, 1650
Sau96I GGNCC 1 cut(s) 505
SchI GAGTC 2 cut(s) 33, 569
ScrFI CCNGG 6 cut(s) 468, 509, 658, 920, 985, 1028
SfaNI GCATC 2 cut(s) 226, 415
SfcI CTRYAG 1 cut(s) 1533
Sfr274I CTCGAG 2 cut(s) 971, 1152
SinI GGWCC 1 cut(s) 505
SlaI CTCGAG 2 cut(s) 971, 1152
SmiMI CAYNNNNRTG 2 cut(s) 327, 1306
SmlI CTYRAG 4 cut(s) 557, 609, 971, 1152
SmoI CTYRAG 4 cut(s) 557, 609, 971, 1152
SphI GCATGC 1 cut(s) 475
SspI AATATT 1 cut(s) 1044
SspMI CTAG 5 cut(s) 500, 641, 777, 794, 1416
StyD4I CCNGG 6 cut(s) 466, 507, 656, 918, 983, 1026
StyI CCWWGG 3 cut(s) 250, 1266, 1293
TaaI ACNGT 1 cut(s) 1261
TaiI ACGT 2 cut(s) 301, 314
TaqI TCGA 6 cut(s) 32, 156, 234, 282, 972, 1153
TfiI GAWTC 3 cut(s) 113, 1133, 1492
Tru1I TTAA 5 cut(s) 315, 452, 1181, 1476, 1571
Tru9I TTAA 5 cut(s) 315, 452, 1181, 1476, 1571
TscAI CASTG 4 cut(s) 297, 933, 1542, 1674
TseFI GTSAC 2 cut(s) 662, 1601
TseI GCWGC 4 cut(s) 491, 689, 811, 1649
Tsp45I GTSAC 2 cut(s) 662, 1601
TspGWI ACGGA 1 cut(s) 399
TspRI CASTG 4 cut(s) 297, 933, 1542, 1674
VpaK11BI GGWCC 1 cut(s) 505
XapI RAATTY 2 cut(s) 70, 437
XbaI TCTAGA 1 cut(s) 640
XceI RCATGY 3 cut(s) 475, 670, 1409
XcmI CCANNNNNNNNNTGG 2 cut(s) 84, 329
XhoI CTCGAG 2 cut(s) 971, 1152
XmiI GTMKAC 1 cut(s) 1235
XmnI GAANNNNTTC 1 cut(s) 54
XspI CTAG 5 cut(s) 500, 641, 777, 794, 1416
Zsp2I ATGCAT 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.