RchiOBHm_Chr5g0059541

(-)-germacrene D synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000053
Physical Location & Seq
Reverse (-)
64506797 .. 64511895
5099 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33614

Sequence Viewer

Length: 1635 bp
ATGTCTTCACAAGTTTTAGCATCTCAATCTCAAGCTCCATATACCAGACCTTCAGCAAATTACACTCCAAGCATTTGGGGTGATCATTTTCTCTCTTATGCTAAGGCGGAAGTGGACTTTAATCTCGAGCAACGAGTCCAAGAACTTAAAAGAGAAGTGAAAATGATCCTCATGGTGCCAGCAAAAGAGCCTTCACAAAAATTGGACTTAGTTGATGACATTCAGCGCCTGGGCATATCTTACCATTTTGAAAATGAGATCGATGAAATTCTGAAACAAATTTACCAGACTACTTACGGTAGTGATGTGGAATATGATGAGGACCTTTACACTACTGCTCTTTGTTTTCGGTTACTTAGACAACACGGTTATAATGTTTCAAGTGATATGTTCAACAAGTACTTCAAGGAGGGTAATGATGAGAAATTTAAGGAATCTCTTTTGAGTGATGTATCAGGACTATTAAGTTTGTACGAAGCCACCCATCTTAGGATACACGGAGAAGACATACTCGAAGAGGCACTAGCCTTCACCACCACACATCTCGAGTCGATGAAAGACAATTTAAGCCCTCCACTTTCGAAACAAGTAGCCCATTCCTTAAATCAGCCACTACGTAAGGGTATCACTAGGGTGGAAGCAAGATATTACTTGTCAATCTACCACGAATGCGATTCACATAATGAAACTCTCCTGACTTTTGCCAAGTTGGATTTCAACCTGTTGCAGTGGTGGAAGGACTTGGACGTGAGAAACGAGCTGCCGTTTACAAGAGACAGAGTTACCGAAGTTTACTTCTGCTGGGCTTCATCAGTCTGCTTTGAACCTGAATATTCCTTTGCTAGGAGGACATTATGCAAAGTTACTGCCCTGGCATCCATTCTCGATGACATTTATGATTTGTATGGCACATTCGAAGAACTAGAGCTCTTTACTAAAGCTATTGAGAGGTGGGATATATGTTTCATGGATGCACTGCCAAGCTATATGAAAGTCTATTATACGGCACTGTTAGACGTGTATACTGTAGTTGAAGAAGAGCTTGCAAAGGAGGGAAAATTGTATCGAATCCACTATGCCATAGAAGCTATGAAAAAGCTAGCTAAATGTTACTTTCTTGAAGCGAAATGGTTCCACCAGAAACATGTACCAACAGCGGATGAGTATATTGCCCTATCATCACTGACCTCAGGCTACCCGCTGCTTGTAACAACCTCTTTTGTTACAATGGGAGATATTGCTACACAAGATTCCTTTGACTGGTTGGCCACTTATCCCAAACCTGTGAAAGGGTGTGCAGTAGTTGCCAGACTTATGGATGACTTAGTGTCTCACAAGTTTGAGCAAAAAAGAGGACATGTTGCGTCAGCTGTAGTGTGCTACATGAAAGAATTTGGTGCCACAGAAGAAGAAGCAATAATTGCAATGCGTAGAAAAGTGAGTGGCGCATGGAAGGACATAAACGAATCGTTCCTCTTCCCCAATGCCATTTCAAGGCCACTACTGACTCGAATTCTTAATTTAGCATGCGTCATGGATGTTGTTTACAAGAATGAAGATGGTTACACTACTGATCAGCATGGTATACTCAAGGATCTTATAACTTCTACGCTTGTCCAACAAGTGCCTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

544

Amino Acids

62.75

Weight (kDa)

5.19

Isoelectric Point (pI)

44.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 25 - 201 7.2e-61 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 246 - 486 6.3e-90 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 287 - 486 1.9e-58 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 372, 1601
AccB1I GGYRCC 2 cut(s) 175, 1397
AccI GTMKAC 2 cut(s) 1022, 1585
AciI CCGC 3 cut(s) 107, 1157, 1199
AclWI GGATC 2 cut(s) 160, 1602
AcoI YGGCCR 1 cut(s) 1266
AcsI RAATTY 5 cut(s) 267, 279, 425, 1391, 1512
AcuI CTGAAG 1 cut(s) 36
AfaI GTAC 3 cut(s) 401, 473, 1149
AfiI CCNNNNNNNGG 5 cut(s) 489, 843, 1260, 1289, 1494
AflIII ACRYGT 3 cut(s) 1017, 1144, 1357
AgsI TTSAA 9 cut(s) 251, 381, 394, 406, 718, 824, 1034, 1121, 1494
AhdI GACNNNNNGTC 1 cut(s) 1327
AjiI CACGTC 2 cut(s) 748, 1018
AjnI CCWGG 2 cut(s) 228, 870
AjuI GAANNNNNNNTTGG 2 cut(s) 698, 730
AleI CACNNNNGTG 1 cut(s) 632
Alw21I GWGCWC 1 cut(s) 930
Alw26I GTCTC 2 cut(s) 768, 1335
AlwI GGATC 2 cut(s) 160, 1602
AlwNI CAGNNNCTG 1 cut(s) 229
Ama87I CYCGRG 2 cut(s) 125, 545
AoxI GGCC 2 cut(s) 1266, 1496
ApeKI GCWGC 2 cut(s) 760, 1201
ApoI RAATTY 5 cut(s) 267, 279, 425, 1391, 1512
Asp700I GAANNNNTTC 1 cut(s) 1130
AspLEI GCGC 2 cut(s) 228, 1448
AspS9I GGNCC 1 cut(s) 322
AsuHPI GGTGA 2 cut(s) 92, 523
AsuII TTCGAA 2 cut(s) 581, 915
AsuNHI GCTAGC 1 cut(s) 1099
AvaI CYCGRG 2 cut(s) 125, 545
AvaII GGWCC 1 cut(s) 322
AxyI CCTNAGG 1 cut(s) 1189
BalI TGGCCA 1 cut(s) 1268
BanI GGYRCC 2 cut(s) 175, 1397
BanII GRGCYC 1 cut(s) 930
BbsI GAAGAC 1 cut(s) 510
Bbv12I GWGCWC 1 cut(s) 930
BbvI GCAGC 2 cut(s) 747, 1188
BccI CCATC 2 cut(s) 492, 1553
BceAI ACGGC 2 cut(s) 748, 1020
BciT130I CCWGG 2 cut(s) 230, 872
BciVI GTATCC 1 cut(s) 486
BclI TGATCA 2 cut(s) 82, 1573
BcoDI GTCTC 2 cut(s) 768, 1335
BfaI CTAG 5 cut(s) 524, 630, 843, 923, 1100
BfmI CTRYAG 2 cut(s) 1026, 1371
BfoI RGCGCY 1 cut(s) 229
BfuI GTATCC 1 cut(s) 486
BisI GCNGC 2 cut(s) 761, 1202
BlsI GCNGC 2 cut(s) 762, 1203
BmcAI AGTACT 1 cut(s) 401
Bme1390I CCNGG 2 cut(s) 230, 872
Bme18I GGWCC 1 cut(s) 322
BmeRI GACNNNNNGTC 1 cut(s) 1327
BmeT110I CYCGRG 2 cut(s) 125, 545
BmgBI CACGTC 2 cut(s) 748, 1018
BmgT120I GGNCC 1 cut(s) 322
BmiI GGNNCC 3 cut(s) 177, 1133, 1399
BmrFI CCNGG 2 cut(s) 230, 872
BmsI GCATC 3 cut(s) 29, 884, 961
BmtI GCTAGC 1 cut(s) 1103
BpiI GAAGAC 1 cut(s) 510
Bpu10I CCTNAGC 1 cut(s) 102
Bpu14I TTCGAA 2 cut(s) 581, 915
BpuEI CTTGAG 2 cut(s) 15, 1574
Bsa29I ATCGAT 1 cut(s) 261
BsaAI YACGTR 1 cut(s) 617
BsaJI CCNNGG 2 cut(s) 229, 870
Bsc4I CCNNNNNNNGG 5 cut(s) 489, 843, 1260, 1289, 1494
Bse1I ACTGG 1 cut(s) 1265
Bse21I CCTNAGG 1 cut(s) 1189
Bse3DI GCAATG 1 cut(s) 1431
BseBI CCWGG 2 cut(s) 230, 872
BseCI ATCGAT 1 cut(s) 261
BseDI CCNNGG 2 cut(s) 229, 870
BseGI GGATG 5 cut(s) 875, 976, 1165, 1324, 1543
BseLI CCNNNNNNNGG 5 cut(s) 489, 843, 1260, 1289, 1494
BseMI GCAATG 1 cut(s) 1431
BseMII CTCAG 1 cut(s) 1203
BseNI ACTGG 1 cut(s) 1265
BseXI GCAGC 2 cut(s) 747, 1188
BseYI CCCAGC 1 cut(s) 801
BsgI GTGCAG 1 cut(s) 1317
BshFI GGCC 2 cut(s) 1268, 1498
BshNI GGYRCC 2 cut(s) 175, 1397
BshVI ATCGAT 1 cut(s) 261
BsiHKAI GWGCWC 1 cut(s) 930
BsiHKCI CYCGRG 2 cut(s) 125, 545
BslI CCNNNNNNNGG 5 cut(s) 489, 843, 1260, 1289, 1494
BsmAI GTCTC 2 cut(s) 768, 1335
BsmI GAATGC 1 cut(s) 674
BsnI GGCC 2 cut(s) 1268, 1498
BsoBI CYCGRG 2 cut(s) 125, 545
Bsp119I TTCGAA 2 cut(s) 581, 915
Bsp1286I GDGCHC 1 cut(s) 930
Bsp143I GATC 5 cut(s) 82, 165, 258, 1573, 1594
BspACI CCGC 3 cut(s) 107, 1157, 1199
BspANI GGCC 2 cut(s) 1268, 1498
BspCNI CTCAG 1 cut(s) 1202
BspDI ATCGAT 1 cut(s) 261
BspLI GGNNCC 3 cut(s) 177, 1133, 1399
BspOI GCTAGC 1 cut(s) 1103
BspPI GGATC 2 cut(s) 160, 1602
BspQI GCTCTTC 1 cut(s) 1032
BspT104I TTCGAA 2 cut(s) 581, 915
BspT107I GGYRCC 2 cut(s) 175, 1397
BsrDI GCAATG 1 cut(s) 1431
BsrI ACTGG 1 cut(s) 1265
BssECI CCNNGG 2 cut(s) 229, 870
BssMI GATC 5 cut(s) 82, 165, 258, 1573, 1594
BssNAI GTATAC 2 cut(s) 1023, 1586
Bst1107I GTATAC 2 cut(s) 1023, 1586
Bst2UI CCWGG 2 cut(s) 230, 872
Bst4CI ACNGT 4 cut(s) 299, 368, 1011, 1027
Bst6I CTCTTC 3 cut(s) 510, 1032, 1481
BstAPI GCANNNNNTGC 2 cut(s) 1304, 1421
BstBAI YACGTR 1 cut(s) 617
BstBI TTCGAA 2 cut(s) 581, 915
BstC8I GCNNGC 4 cut(s) 180, 1044, 1101, 1528
BstDEI CTNAG 6 cut(s) 102, 208, 356, 488, 1189, 1324
BstENI CCTNNNNNAGG 2 cut(s) 841, 1287
BstF5I GGATG 5 cut(s) 875, 976, 1165, 1324, 1543
BstH2I RGCGCY 1 cut(s) 229
BstHHI GCGC 2 cut(s) 228, 1448
BstKTI GATC 5 cut(s) 85, 168, 261, 1576, 1597
BstMAI GTCTC 2 cut(s) 768, 1335
BstMBI GATC 5 cut(s) 82, 165, 258, 1573, 1594
BstMWI GCNNNNNNNGC 3 cut(s) 1085, 1304, 1421
BstNI CCWGG 2 cut(s) 230, 872
BstNSI RCATGY 3 cut(s) 1148, 1361, 1530
BstSCI CCNGG 2 cut(s) 228, 870
BstSFI CTRYAG 2 cut(s) 1026, 1371
BstSNI TACGTA 1 cut(s) 617
BstV1I GCAGC 2 cut(s) 747, 1188
BstV2I GAAGAC 1 cut(s) 510
BstX2I RGATCY 1 cut(s) 1594
BstXI CCANNNNNNTGG 2 cut(s) 75, 1315
BstYI RGATCY 1 cut(s) 1594
BstZ17I GTATAC 2 cut(s) 1023, 1586
Bsu15I ATCGAT 1 cut(s) 261
Bsu36I CCTNAGG 1 cut(s) 1189
BsuI GTATCC 1 cut(s) 486
BsuRI GGCC 2 cut(s) 1268, 1498
BsuTUI ATCGAT 1 cut(s) 261
BtrI CACGTC 2 cut(s) 748, 1018
BtsCI GGATG 5 cut(s) 875, 976, 1165, 1324, 1543
BtsI GCAGTG 2 cut(s) 734, 974
BtsIMutI CAGTG 4 cut(s) 734, 974, 1007, 1181
Cac8I GCNNGC 4 cut(s) 180, 1044, 1101, 1528
CaiI CAGNNNCTG 1 cut(s) 229
CfoI GCGC 2 cut(s) 228, 1448
Cfr13I GGNCC 1 cut(s) 322
ClaI ATCGAT 1 cut(s) 261
CseI GACGC 2 cut(s) 1353, 1519
Csp6I GTAC 3 cut(s) 400, 472, 1148
CviAII CATG 9 cut(s) 172, 967, 1145, 1358, 1384, 1449, 1527, 1534, 1580
CviQI GTAC 3 cut(s) 400, 472, 1148
DdeI CTNAG 6 cut(s) 102, 208, 356, 488, 1189, 1324
DpnI GATC 5 cut(s) 84, 167, 260, 1575, 1596
DpnII GATC 5 cut(s) 82, 165, 258, 1573, 1594
DriI GACNNNNNGTC 1 cut(s) 1327
EaeI YGGCCR 1 cut(s) 1266
Eam1104I CTCTTC 3 cut(s) 510, 1032, 1481
Eam1105I GACNNNNNGTC 1 cut(s) 1327
EarI CTCTTC 3 cut(s) 510, 1032, 1481
EciI GGCGGA 1 cut(s) 122
Ecl136II GAGCTC 1 cut(s) 928
Eco105I TACGTA 1 cut(s) 617
Eco24I GRGCYC 1 cut(s) 930
Eco47I GGWCC 1 cut(s) 322
Eco53kI GAGCTC 1 cut(s) 928
Eco57I CTGAAG 1 cut(s) 36
Eco81I CCTNAGG 1 cut(s) 1189
Eco88I CYCGRG 2 cut(s) 125, 545
EcoICRI GAGCTC 1 cut(s) 928
EcoNI CCTNNNNNAGG 2 cut(s) 841, 1287
EcoO109I RGGNCCY 1 cut(s) 322
EcoRI GAATTC 1 cut(s) 1512
EcoRII CCWGG 2 cut(s) 228, 870
EcoT38I GRGCYC 1 cut(s) 930
FaeI CATG 9 cut(s) 175, 970, 1148, 1361, 1387, 1452, 1530, 1537, 1583
FalI AAGNNNNNCTT 2 cut(s) 1026, 1058
FatI CATG 9 cut(s) 171, 966, 1144, 1357, 1383, 1448, 1526, 1533, 1579
FauI CCCGC 1 cut(s) 1206
FbaI TGATCA 2 cut(s) 82, 1573
FblI GTMKAC 2 cut(s) 1022, 1585
Fnu4HI GCNGC 2 cut(s) 761, 1202
FokI GGATG 5 cut(s) 862, 983, 1172, 1331, 1550
FriOI GRGCYC 1 cut(s) 930
Fsp4HI GCNGC 2 cut(s) 761, 1202
FspBI CTAG 5 cut(s) 524, 630, 843, 923, 1100
GlaI GCGC 2 cut(s) 227, 1447
GluI GCNGC 2 cut(s) 761, 1202
GsaI CCCAGC 1 cut(s) 805
HaeII RGCGCY 1 cut(s) 229
HaeIII GGCC 2 cut(s) 1268, 1498
HgaI GACGC 2 cut(s) 1353, 1519
HhaI GCGC 2 cut(s) 228, 1448
Hin1II CATG 9 cut(s) 175, 970, 1148, 1361, 1387, 1452, 1530, 1537, 1583
Hin6I GCGC 2 cut(s) 226, 1446
HinP1I GCGC 2 cut(s) 226, 1446
HinfI GANTC 8 cut(s) 135, 434, 548, 674, 1068, 1250, 1466, 1507
HphI GGTGA 2 cut(s) 92, 523
Hpy166II GTNNAC 6 cut(s) 115, 768, 793, 1023, 1546, 1586
Hpy188I TCNGA 1 cut(s) 273
Hpy188III TCNNGA 6 cut(s) 125, 456, 545, 694, 884, 1118
Hpy8I GTNNAC 6 cut(s) 115, 768, 793, 1023, 1546, 1586
HpyAV CCTTC 5 cut(s) 60, 201, 538, 730, 1447
HpyCH4III ACNGT 4 cut(s) 299, 368, 1011, 1027
HpyCH4IV ACGT 3 cut(s) 616, 747, 1017
HpyCH4V TGCA 6 cut(s) 727, 858, 974, 1046, 1298, 1424
HpyF10VI GCNNNNNNNGC 3 cut(s) 1085, 1304, 1421
HpyF3I CTNAG 6 cut(s) 102, 208, 356, 488, 1189, 1324
HpySE526I ACGT 3 cut(s) 616, 747, 1017
Hsp92II CATG 9 cut(s) 175, 970, 1148, 1361, 1387, 1452, 1530, 1537, 1583
HspAI GCGC 2 cut(s) 226, 1446
Ksp22I TGATCA 2 cut(s) 82, 1573
Kzo9I GATC 5 cut(s) 82, 165, 258, 1573, 1594
LguI GCTCTTC 1 cut(s) 1032
LmnI GCTCC 1 cut(s) 40
Lsp1109I GCAGC 2 cut(s) 747, 1188
LweI GCATC 3 cut(s) 29, 884, 961
MaeI CTAG 5 cut(s) 524, 630, 843, 923, 1100
MaeII ACGT 3 cut(s) 616, 747, 1017
MaeIII GTNAC 7 cut(s) 351, 781, 862, 1109, 1207, 1222, 1562
MalI GATC 5 cut(s) 84, 167, 260, 1575, 1596
MboI GATC 5 cut(s) 82, 165, 258, 1573, 1594
MboII GAAGA 9 cut(s) 515, 527, 929, 1046, 1049, 1418, 1421, 1468, 1568
MflI RGATCY 1 cut(s) 1594
MhlI GDGCHC 1 cut(s) 930
MlsI TGGCCA 1 cut(s) 1268
MluNI TGGCCA 1 cut(s) 1268
MlyI GAGTC 3 cut(s) 144, 557, 1501
MmeI TCCRAC 1 cut(s) 690
Mox20I TGGCCA 1 cut(s) 1268
MroXI GAANNNNTTC 1 cut(s) 1130
MscI TGGCCA 1 cut(s) 1268
MseI TTAA 7 cut(s) 120, 147, 429, 464, 566, 602, 1518
MslI CAYNNNNRTG 1 cut(s) 632
Msp20I TGGCCA 1 cut(s) 1268
MspA1I CMGCKG 3 cut(s) 1157, 1201, 1370
MspR9I CCNGG 2 cut(s) 230, 872
Mva1269I GAATGC 1 cut(s) 674
MvaI CCWGG 2 cut(s) 230, 872
MwoI GCNNNNNNNGC 3 cut(s) 1085, 1304, 1421
NdeII GATC 5 cut(s) 82, 165, 258, 1573, 1594
NheI GCTAGC 1 cut(s) 1099
NlaIII CATG 9 cut(s) 175, 970, 1148, 1361, 1387, 1452, 1530, 1537, 1583
NlaIV GGNNCC 3 cut(s) 177, 1133, 1399
NspI RCATGY 3 cut(s) 1148, 1361, 1530
NspV TTCGAA 2 cut(s) 581, 915
OliI CACNNNNGTG 1 cut(s) 632
PaeI GCATGC 1 cut(s) 1530
PaeR7I CTCGAG 2 cut(s) 125, 545
PciI ACATGT 2 cut(s) 1144, 1357
PciSI GCTCTTC 1 cut(s) 1032
PcsI WCGNNNNNNNCGW 1 cut(s) 753
PctI GAATGC 1 cut(s) 674
PdmI GAANNNNTTC 1 cut(s) 1130
PfeI GAWTC 5 cut(s) 434, 674, 1068, 1250, 1466
PkrI GCNGC 2 cut(s) 762, 1203
PleI GAGTC 3 cut(s) 143, 556, 1501
PpsI GAGTC 3 cut(s) 143, 556, 1501
Ppu21I YACGTR 1 cut(s) 617
PpuMI RGGWCCY 1 cut(s) 322
PscI ACATGT 2 cut(s) 1144, 1357
PsiI TTATAA 2 cut(s) 372, 1601
Psp124BI GAGCTC 1 cut(s) 930
Psp5II RGGWCCY 1 cut(s) 322
Psp6I CCWGG 2 cut(s) 228, 870
PspFI CCCAGC 1 cut(s) 801
PspGI CCWGG 2 cut(s) 228, 870
PspN4I GGNNCC 3 cut(s) 177, 1133, 1399
PspPI GGNCC 1 cut(s) 322
PspPPI RGGWCCY 1 cut(s) 322
PstNI CAGNNNCTG 1 cut(s) 229
PsuI RGATCY 1 cut(s) 1594
PvuII CAGCTG 1 cut(s) 1370
RsaI GTAC 3 cut(s) 401, 473, 1149
RsaNI GTAC 3 cut(s) 400, 472, 1148
RseI CAYNNNNRTG 1 cut(s) 632
SacI GAGCTC 1 cut(s) 930
SapI GCTCTTC 1 cut(s) 1032
SaqAI TTAA 7 cut(s) 120, 147, 429, 464, 566, 602, 1518
SatI GCNGC 2 cut(s) 761, 1202
Sau3AI GATC 5 cut(s) 82, 165, 258, 1573, 1594
Sau96I GGNCC 1 cut(s) 322
ScaI AGTACT 1 cut(s) 401
SchI GAGTC 3 cut(s) 144, 557, 1501
ScrFI CCNGG 2 cut(s) 230, 872
SduI GDGCHC 1 cut(s) 930
SfaNI GCATC 3 cut(s) 29, 884, 961
SfcI CTRYAG 2 cut(s) 1026, 1371
Sfr274I CTCGAG 2 cut(s) 125, 545
SfuI TTCGAA 2 cut(s) 581, 915
SinI GGWCC 1 cut(s) 322
SlaI CTCGAG 2 cut(s) 125, 545
SmiMI CAYNNNNRTG 1 cut(s) 632
SmlI CTYRAG 4 cut(s) 30, 125, 545, 1589
SmoI CTYRAG 4 cut(s) 30, 125, 545, 1589
SnaBI TACGTA 1 cut(s) 617
SphI GCATGC 1 cut(s) 1530
SsiI CCGC 3 cut(s) 107, 1157, 1199
SspI AATATT 1 cut(s) 833
SspMI CTAG 5 cut(s) 524, 630, 843, 923, 1100
SstI GAGCTC 1 cut(s) 930
StyD4I CCNGG 2 cut(s) 228, 870
TaaI ACNGT 4 cut(s) 299, 368, 1011, 1027
TaiI ACGT 3 cut(s) 619, 750, 1020
TatI WGTACW 1 cut(s) 399
TfiI GAWTC 5 cut(s) 434, 674, 1068, 1250, 1466
Tru1I TTAA 7 cut(s) 120, 147, 429, 464, 566, 602, 1518
Tru9I TTAA 7 cut(s) 120, 147, 429, 464, 566, 602, 1518
TscAI CASTG 4 cut(s) 734, 981, 1014, 1188
TseI GCWGC 2 cut(s) 760, 1201
TspDTI ATGAA 9 cut(s) 279, 569, 699, 798, 955, 1004, 1106, 1400, 1569
TspGWI ACGGA 1 cut(s) 513
TspRI CASTG 4 cut(s) 734, 981, 1014, 1188
VpaK11BI GGWCC 1 cut(s) 322
XagI CCTNNNNNAGG 2 cut(s) 841, 1287
XapI RAATTY 5 cut(s) 267, 279, 425, 1391, 1512
XceI RCATGY 3 cut(s) 1148, 1361, 1530
XhoI CTCGAG 2 cut(s) 125, 545
XmiI GTMKAC 2 cut(s) 1022, 1585
XmnI GAANNNNTTC 1 cut(s) 1130
XspI CTAG 5 cut(s) 524, 630, 843, 923, 1100
ZrmI AGTACT 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.