Rmu_ssc0000062.1_g000038

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000062.1
OG0000053
Physical Location & Seq
Reverse (-)
216694 .. 218901
2208 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000062.1_g000038.1.cds

Sequence Viewer

Length: 1668 bp
atgtcaatgtctttccaagcagctgttctttcatcaaatgctaagcctgaaattattcgcaggacggcaaattatcaccctagtatttggggagatcggttcatcaactgcgattccaaagacaatataagtcatgcccgtaggcaacagcaagttgaggagctgaaagcagtagtgaagagagaagtattcaaaaatctggcaactgagttttcacatcaactgaagttaatcgatgcaatccagtgccttggtgtggcttaccattttgaaaccgaaatagaagaagccctagagcatatgcatgccaaatatcatgactatgatggtgacctatacaatgttgctcttggatttcggctactcaggcagcaaggatacagtgtttcatgtgatatattcaacaaattcaaagatgaaaatggtagcttcaaggaatgtttaatcgatgatgtgtcgggtatgctaagcttttacgaagcagcacatcttagggtgcggggagaagagatactagacgaggctgttgttttcaccaccactaaccttgagtcggaaacaattggtgtaaggtacccactagcagcacaagtaagtcaggccttacatagacctctccggaaagatctagagagggtttgtgctaggcgttacatgtcaatataccaagacgaagcctcacacaatgaagctttgctgaaacttgccaagttggatttcaatctcgttcaatctttgcacaaagaggagctcagtgagattactaagtggtggaaggagctagacttcgccaggaagctgtcatttgcaagagataggatggtggagttgtacttttggatagtgggagtctactttgaaccccactactcttctgcacgaaaaattctaacaaaagtgattgcgctggtatcagtaatggatgatatctacgatgcattcgctacctttgaagaattggagatatttactgcagctatggagaggtgggatgtcaaatgcatggatgacctcccagactatatgcgaatattttatagtgcacttttgaatgtggtcactgaaattgaggaagagatggcaaaggaaggaagatcataccgagtttattatgtaaaggaatctttgaaaggtgtcgctcgagcttactttgaagaggccaaatggttcaaagacgagcgaatcccaaccgtggaggagtatttacgttatgcaatattttcaactggttaccctatgctggcaactgtatccttggtcggcatgggtgatattgtaacagaggagacatttcaatggctgttcaatgatcctaaaattgtcatagcttccacaacactttttcgactcatggatgacgtagtcaccaccaagtttgagaaagaacgagggcatgttgcttcgagtattgattgctacatgaaacaacatggggtctcagaggaagaggtgattgaggtgtttaacaagcaggttgtggattactggaaggatataaacgaagagtttctaaggccaactgctgttccaatgcctgtgcttttgcgtgttcttaacttaacaaaagtagctgatctgctttacaaaggagaagacggctacacccgtgttggaaaagttatgaaagaatgtgttgcttcacatttcattgatcctgtgccactatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

555

Amino Acids

64.48

Weight (kDa)

5.25

Isoelectric Point (pI)

49.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1462
Acc65I GGTACC 1 cut(s) 573
AccB1I GGYRCC 1 cut(s) 573
AccI GTMKAC 1 cut(s) 852
AccIII TCCGGA 1 cut(s) 618
AciI CCGC 1 cut(s) 499
AclWI GGATC 2 cut(s) 1304, 1646
AcsI RAATTY 2 cut(s) 407, 885
AcuI CTGAAG 1 cut(s) 245
AfaI GTAC 2 cut(s) 575, 833
AfiI CCNNNNNNNGG 4 cut(s) 256, 553, 1192, 1240
AflIII ACRYGT 1 cut(s) 654
AjnI CCWGG 1 cut(s) 791
AjuI GAANNNNNNNTTGG 4 cut(s) 9, 41, 701, 733
Alw21I GWGCWC 2 cut(s) 753, 1045
Alw26I GTCTC 2 cut(s) 1280, 1441
Alw44I GTGCAC 1 cut(s) 1041
AlwI GGATC 2 cut(s) 1304, 1646
Ama87I CYCGRG 1 cut(s) 1140
Aor13HI TCCGGA 1 cut(s) 618
AoxI GGCC 3 cut(s) 600, 1158, 1514
ApaLI GTGCAC 1 cut(s) 1041
ApeKI GCWGC 5 cut(s) 20, 370, 482, 584, 974
ApoI RAATTY 2 cut(s) 407, 885
Asp700I GAANNNNTTC 2 cut(s) 54, 1506
Asp718I GGTACC 1 cut(s) 573
AspLEI GCGC 1 cut(s) 907
AsuHPI GGTGA 6 cut(s) 68, 341, 526, 1280, 1357, 1462
AvaI CYCGRG 1 cut(s) 1140
BaeGI GKGCMC 1 cut(s) 1045
BanI GGYRCC 1 cut(s) 573
BanII GRGCYC 1 cut(s) 753
BbsI GAAGAC 1 cut(s) 1599
Bbv12I GWGCWC 2 cut(s) 753, 1045
BbvI GCAGC 5 cut(s) 32, 382, 494, 596, 986
BccI CCATC 3 cut(s) 320, 814, 1072
BceAI ACGGC 2 cut(s) 81, 1612
BciT130I CCWGG 1 cut(s) 793
BciVI GTATCC 2 cut(s) 371, 1261
BcoDI GTCTC 2 cut(s) 1280, 1441
BfaI CTAG 7 cut(s) 81, 293, 515, 581, 629, 645, 782
BfmI CTRYAG 1 cut(s) 972
BfuAI ACCTGC 1 cut(s) 1462
BfuI GTATCC 2 cut(s) 371, 1261
BglII AGATCT 1 cut(s) 625
BisI GCNGC 5 cut(s) 21, 371, 483, 585, 975
BlpI GCTNAGC 2 cut(s) 42, 467
BlsI GCNGC 5 cut(s) 22, 372, 484, 586, 976
Bme1390I CCNGG 1 cut(s) 793
BmeT110I CYCGRG 1 cut(s) 1140
BmiI GGNNCC 1 cut(s) 575
BmrFI CCNGG 1 cut(s) 793
BmsI GCATC 2 cut(s) 226, 925
BpiI GAAGAC 1 cut(s) 1599
Bpu1102I GCTNAGC 2 cut(s) 42, 467
BpuEI CTTGAG 1 cut(s) 569
Bsa29I ATCGAT 2 cut(s) 234, 447
BsaBI GATNNNNATC 1 cut(s) 720
BsaI GGTCTC 1 cut(s) 1441
BsaJI CCNNGG 3 cut(s) 250, 1191, 1254
BsaWI WCCGGW 1 cut(s) 618
Bsc4I CCNNNNNNNGG 4 cut(s) 256, 553, 1192, 1240
Bse1I ACTGG 3 cut(s) 244, 1231, 1490
Bse8I GATNNNNATC 1 cut(s) 720
BseAI TCCGGA 1 cut(s) 618
BseBI CCWGG 1 cut(s) 793
BseCI ATCGAT 2 cut(s) 234, 447
BseDI CCNNGG 3 cut(s) 250, 1191, 1254
BseGI GGATG 5 cut(s) 825, 928, 997, 1012, 1360
BseJI GATNNNNATC 1 cut(s) 720
BseLI CCNNNNNNNGG 4 cut(s) 256, 553, 1192, 1240
BseMII CTCAG 4 cut(s) 198, 379, 766, 1452
BseNI ACTGG 3 cut(s) 244, 1231, 1490
BseRI GAGGAG 4 cut(s) 173, 761, 1211, 1298
BseSI GKGCMC 1 cut(s) 1045
BseXI GCAGC 5 cut(s) 32, 382, 494, 596, 986
BsgI GTGCAG 1 cut(s) 861
BshFI GGCC 3 cut(s) 602, 1160, 1516
BshNI GGYRCC 1 cut(s) 573
BshVI ATCGAT 2 cut(s) 234, 447
BsiHKAI GWGCWC 2 cut(s) 753, 1045
BsiHKCI CYCGRG 1 cut(s) 1140
BsiSI CCGG 1 cut(s) 619
BslI CCNNNNNNNGG 4 cut(s) 256, 553, 1192, 1240
BsmAI GTCTC 2 cut(s) 1280, 1441
BsmI GAATGC 1 cut(s) 938
BsnI GGCC 3 cut(s) 602, 1160, 1516
Bso31I GGTCTC 1 cut(s) 1441
BsoBI CYCGRG 1 cut(s) 1140
Bsp1286I GDGCHC 2 cut(s) 753, 1045
Bsp13I TCCGGA 1 cut(s) 618
Bsp143I GATC 6 cut(s) 94, 625, 1094, 1309, 1573, 1651
Bsp1720I GCTNAGC 2 cut(s) 42, 467
BspACI CCGC 1 cut(s) 499
BspANI GGCC 3 cut(s) 602, 1160, 1516
BspCNI CTCAG 4 cut(s) 199, 378, 765, 1451
BspDI ATCGAT 2 cut(s) 234, 447
BspEI TCCGGA 1 cut(s) 618
BspHI TCATGA 1 cut(s) 316
BspLI GGNNCC 1 cut(s) 575
BspMAI CTGCAG 1 cut(s) 976
BspMI ACCTGC 1 cut(s) 1462
BspPI GGATC 2 cut(s) 1304, 1646
BspT107I GGYRCC 1 cut(s) 573
BspTNI GGTCTC 1 cut(s) 1441
BsrI ACTGG 3 cut(s) 244, 1231, 1490
BssECI CCNNGG 3 cut(s) 250, 1191, 1254
BssMI GATC 6 cut(s) 94, 625, 1094, 1309, 1573, 1651
BssT1I CCWWGG 2 cut(s) 250, 1254
Bst2UI CCWGG 1 cut(s) 793
Bst4CI ACNGT 3 cut(s) 383, 1192, 1249
Bst6I CTCTTC 7 cut(s) 173, 501, 877, 1068, 1149, 1440, 1497
BstC8I GCNNGC 2 cut(s) 306, 1242
BstDEI CTNAG 9 cut(s) 42, 207, 365, 467, 491, 752, 765, 1438, 1511
BstDSI CCRYGG 1 cut(s) 1191
BstEII GGTNACC 2 cut(s) 329, 1229
BstF5I GGATG 5 cut(s) 825, 928, 997, 1012, 1360
BstHHI GCGC 1 cut(s) 907
BstKTI GATC 6 cut(s) 97, 628, 1097, 1312, 1576, 1654
BstMAI GTCTC 2 cut(s) 1280, 1441
BstMBI GATC 6 cut(s) 94, 625, 1094, 1309, 1573, 1651
BstMWI GCNNNNNNNGC 1 cut(s) 367
BstNI CCWGG 1 cut(s) 793
BstNSI RCATGY 3 cut(s) 308, 658, 1397
BstPI GGTNACC 2 cut(s) 329, 1229
BstSCI CCNGG 1 cut(s) 791
BstSFI CTRYAG 1 cut(s) 972
BstSLI GKGCMC 1 cut(s) 1045
BstV1I GCAGC 5 cut(s) 32, 382, 494, 596, 986
BstV2I GAAGAC 1 cut(s) 1599
BstX2I RGATCY 1 cut(s) 625
BstXI CCANNNNNNTGG 1 cut(s) 251
BstYI RGATCY 1 cut(s) 625
Bsu15I ATCGAT 2 cut(s) 234, 447
BsuI GTATCC 2 cut(s) 371, 1261
BsuRI GGCC 3 cut(s) 602, 1160, 1516
BsuTUI ATCGAT 2 cut(s) 234, 447
BtgI CCRYGG 1 cut(s) 1191
BtsCI GGATG 5 cut(s) 825, 928, 997, 1012, 1360
BtsIMutI CAGTG 4 cut(s) 251, 388, 760, 1059
BveI ACCTGC 1 cut(s) 1462
Cac8I GCNNGC 2 cut(s) 306, 1242
CciI TCATGA 1 cut(s) 316
CfoI GCGC 1 cut(s) 907
ClaI ATCGAT 2 cut(s) 234, 447
Csp6I GTAC 2 cut(s) 574, 832
CspCI CAANNNNNGTGG 4 cut(s) 529, 564, 1357, 1392
CviQI GTAC 2 cut(s) 574, 832
DdeI CTNAG 9 cut(s) 42, 207, 365, 467, 491, 752, 765, 1438, 1511
DpnI GATC 6 cut(s) 96, 627, 1096, 1311, 1575, 1653
DpnII GATC 6 cut(s) 94, 625, 1094, 1309, 1573, 1651
Eam1104I CTCTTC 7 cut(s) 173, 501, 877, 1068, 1149, 1440, 1497
EarI CTCTTC 7 cut(s) 173, 501, 877, 1068, 1149, 1440, 1497
Ecl136II GAGCTC 1 cut(s) 751
Eco130I CCWWGG 2 cut(s) 250, 1254
Eco147I AGGCCT 1 cut(s) 602
Eco24I GRGCYC 1 cut(s) 753
Eco31I GGTCTC 1 cut(s) 1441
Eco32I GATATC 1 cut(s) 928
Eco53kI GAGCTC 1 cut(s) 751
Eco57I CTGAAG 1 cut(s) 245
Eco88I CYCGRG 1 cut(s) 1140
Eco91I GGTNACC 2 cut(s) 329, 1229
EcoICRI GAGCTC 1 cut(s) 751
EcoO65I GGTNACC 2 cut(s) 329, 1229
EcoRII CCWGG 1 cut(s) 791
EcoRV GATATC 1 cut(s) 928
EcoT14I CCWWGG 2 cut(s) 250, 1254
EcoT22I ATGCAT 3 cut(s) 306, 940, 1004
EcoT38I GRGCYC 1 cut(s) 753
ErhI CCWWGG 2 cut(s) 250, 1254
FauI CCCGC 1 cut(s) 492
FauNDI CATATG 1 cut(s) 300
FblI GTMKAC 1 cut(s) 852
Fnu4HI GCNGC 5 cut(s) 21, 371, 483, 585, 975
FokI GGATG 5 cut(s) 832, 935, 1004, 1019, 1367
FriOI GRGCYC 1 cut(s) 753
Fsp4HI GCNGC 5 cut(s) 21, 371, 483, 585, 975
FspBI CTAG 7 cut(s) 81, 293, 515, 581, 629, 645, 782
GlaI GCGC 1 cut(s) 906
GluI GCNGC 5 cut(s) 21, 371, 483, 585, 975
HaeIII GGCC 3 cut(s) 602, 1160, 1516
HapII CCGG 1 cut(s) 619
HhaI GCGC 1 cut(s) 907
Hin6I GCGC 1 cut(s) 905
HinP1I GCGC 1 cut(s) 905
HindIII AAGCTT 2 cut(s) 469, 690
HinfI GANTC 6 cut(s) 113, 551, 849, 1121, 1182, 1347
HpaII CCGG 1 cut(s) 619
HphI GGTGA 6 cut(s) 68, 341, 526, 1280, 1357, 1462
Hpy166II GTNNAC 2 cut(s) 853, 1043
Hpy188I TCNGA 2 cut(s) 556, 1441
Hpy188III TCNNGA 3 cut(s) 317, 619, 629
Hpy8I GTNNAC 2 cut(s) 853, 1043
HpyAV CCTTC 3 cut(s) 769, 1082, 1483
HpyCH4III ACNGT 3 cut(s) 383, 1192, 1249
HpyCH4IV ACGT 2 cut(s) 1207, 1359
HpyF10VI GCNNNNNNNGC 1 cut(s) 367
HpyF3I CTNAG 9 cut(s) 42, 207, 365, 467, 491, 752, 765, 1438, 1511
HpySE526I ACGT 2 cut(s) 1207, 1359
HspAI GCGC 1 cut(s) 905
Kpn2I TCCGGA 1 cut(s) 618
KpnI GGTACC 1 cut(s) 577
Kzo9I GATC 6 cut(s) 94, 625, 1094, 1309, 1573, 1651
LmnI GCTCC 3 cut(s) 160, 748, 778
Lsp1109I GCAGC 5 cut(s) 32, 382, 494, 596, 986
LweI GCATC 2 cut(s) 226, 925
MaeI CTAG 7 cut(s) 81, 293, 515, 581, 629, 645, 782
MaeII ACGT 2 cut(s) 1207, 1359
MaeIII GTNAC 6 cut(s) 329, 650, 1057, 1229, 1276, 1363
MalI GATC 6 cut(s) 96, 627, 1096, 1311, 1575, 1653
MboI GATC 6 cut(s) 94, 625, 1094, 1309, 1573, 1651
MfeI CAATTG 1 cut(s) 561
MflI RGATCY 1 cut(s) 625
MhlI GDGCHC 2 cut(s) 753, 1045
MluCI AATT 8 cut(s) 51, 70, 407, 561, 885, 956, 1065, 1317
MlyI GAGTC 3 cut(s) 560, 858, 1341
MmeI TCCRAC 3 cut(s) 534, 693, 1591
Mph1103I ATGCAT 3 cut(s) 306, 940, 1004
MroI TCCGGA 1 cut(s) 618
MroXI GAANNNNTTC 2 cut(s) 54, 1506
MseI TTAA 5 cut(s) 230, 443, 1464, 1554, 1559
MslI CAYNNNNRTG 3 cut(s) 303, 321, 1294
MspA1I CMGCKG 1 cut(s) 23
MspI CCGG 1 cut(s) 619
MspR9I CCNGG 1 cut(s) 793
MunI CAATTG 1 cut(s) 561
Mva1269I GAATGC 1 cut(s) 938
MvaI CCWGG 1 cut(s) 793
MwoI GCNNNNNNNGC 1 cut(s) 367
NdeI CATATG 1 cut(s) 300
NdeII GATC 6 cut(s) 94, 625, 1094, 1309, 1573, 1651
NlaIV GGNNCC 1 cut(s) 575
NmuCI GTSAC 3 cut(s) 329, 1057, 1363
NsiI ATGCAT 3 cut(s) 306, 940, 1004
NspI RCATGY 3 cut(s) 308, 658, 1397
PaeI GCATGC 1 cut(s) 308
PaeR7I CTCGAG 1 cut(s) 1140
PagI TCATGA 1 cut(s) 316
PceI AGGCCT 1 cut(s) 602
PciI ACATGT 1 cut(s) 654
PctI GAATGC 1 cut(s) 938
PdmI GAANNNNTTC 2 cut(s) 54, 1506
PfeI GAWTC 3 cut(s) 113, 1121, 1182
PflFI GACNNNGTC 1 cut(s) 1361
PkrI GCNGC 5 cut(s) 22, 372, 484, 586, 976
PleI GAGTC 3 cut(s) 559, 857, 1341
PpsI GAGTC 3 cut(s) 559, 857, 1341
PscI ACATGT 1 cut(s) 654
Psp124BI GAGCTC 1 cut(s) 753
Psp6I CCWGG 1 cut(s) 791
PspEI GGTNACC 2 cut(s) 329, 1229
PspGI CCWGG 1 cut(s) 791
PspN4I GGNNCC 1 cut(s) 575
PspXI VCTCGAGB 1 cut(s) 1140
PstI CTGCAG 1 cut(s) 976
PsuI RGATCY 1 cut(s) 625
PsyI GACNNNGTC 1 cut(s) 1361
PvuII CAGCTG 1 cut(s) 23
RsaI GTAC 2 cut(s) 575, 833
RsaNI GTAC 2 cut(s) 574, 832
RseI CAYNNNNRTG 3 cut(s) 303, 321, 1294
SacI GAGCTC 1 cut(s) 753
SaqAI TTAA 5 cut(s) 230, 443, 1464, 1554, 1559
SatI GCNGC 5 cut(s) 21, 371, 483, 585, 975
Sau3AI GATC 6 cut(s) 94, 625, 1094, 1309, 1573, 1651
SchI GAGTC 3 cut(s) 560, 858, 1341
ScrFI CCNGG 1 cut(s) 793
SduI GDGCHC 2 cut(s) 753, 1045
SfaNI GCATC 2 cut(s) 226, 925
SfcI CTRYAG 1 cut(s) 972
Sfr274I CTCGAG 1 cut(s) 1140
SlaI CTCGAG 1 cut(s) 1140
SmiMI CAYNNNNRTG 3 cut(s) 303, 321, 1294
SmlI CTYRAG 2 cut(s) 548, 1140
SmoI CTYRAG 2 cut(s) 548, 1140
SphI GCATGC 1 cut(s) 308
Sse9I AATT 8 cut(s) 51, 70, 407, 561, 885, 956, 1065, 1317
SseBI AGGCCT 1 cut(s) 602
SsiI CCGC 1 cut(s) 499
SspI AATATT 2 cut(s) 1032, 1218
SspMI CTAG 7 cut(s) 81, 293, 515, 581, 629, 645, 782
SstI GAGCTC 1 cut(s) 753
StuI AGGCCT 1 cut(s) 602
StyD4I CCNGG 1 cut(s) 791
StyI CCWWGG 2 cut(s) 250, 1254
TaaI ACNGT 3 cut(s) 383, 1192, 1249
TaiI ACGT 2 cut(s) 1210, 1362
TaqI TCGA 5 cut(s) 234, 447, 1141, 1345, 1403
TasI AATT 8 cut(s) 51, 70, 407, 561, 885, 956, 1065, 1317
TatI WGTACW 1 cut(s) 831
TfiI GAWTC 3 cut(s) 113, 1121, 1182
Tru1I TTAA 5 cut(s) 230, 443, 1464, 1554, 1559
Tru9I TTAA 5 cut(s) 230, 443, 1464, 1554, 1559
TscAI CASTG 4 cut(s) 251, 388, 760, 1066
TseFI GTSAC 3 cut(s) 329, 1057, 1363
TseI GCWGC 5 cut(s) 20, 370, 482, 584, 974
Tsp45I GTSAC 3 cut(s) 329, 1057, 1363
TspDTI ATGAA 8 cut(s) 21, 91, 378, 432, 702, 1436, 1636, 1637
TspRI CASTG 4 cut(s) 251, 388, 760, 1066
Tth111I GACNNNGTC 1 cut(s) 1361
VneI GTGCAC 1 cut(s) 1041
XapI RAATTY 2 cut(s) 407, 885
XbaI TCTAGA 1 cut(s) 628
XceI RCATGY 3 cut(s) 308, 658, 1397
XhoI CTCGAG 1 cut(s) 1140
XmiI GTMKAC 1 cut(s) 852
XmnI GAANNNNTTC 2 cut(s) 54, 1506
XspI CTAG 7 cut(s) 81, 293, 515, 581, 629, 645, 782
Zsp2I ATGCAT 3 cut(s) 306, 940, 1004
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.