Rw5G040050

(-)-germacrene D synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000053
Physical Location & Seq
Reverse (-)
70749861 .. 70752152
2292 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G040050.1

Sequence Viewer

Length: 1674 bp
ATGTCTCTCAACGGGGTCAATATAGCTTCTCAAGCCCCTCCAACACTGAATGCCTCTACTGATGCCGATAGTCGACGTTCGGCAAATTTCCATCCCAGCATTTGGGGAGACCAGTTTCTGTCTTGTAACAACACTATGGAAACTGACATAAATGGTGAGCAACATGTCCAACAACTGAAGGAAGAAGTGAAGAGAGTGCTAATGGCTCCGCCAGTTGAAACTTGTTTAGGAAAATTGGAGTTGATTGATGAGATTCAACGCTTGGGCGTGTCTTACCATTTTGAATACGAGATTGACCAAACTATGCAGCAAATTCACGACAATTTTAATGGTGACTGTGATGGTGATCTGCACACTTGTGCTCTTCGTTTTCGCTTGTTGAGGCAGCATGGTTACAATGTTTCATGCGATATGTTCAACAAGTTTGACTACACATCGCTTCATGATGATGTAGTGGGGCTACAAAGTTTGTATGAAGCCACACATCTTAGAGTACGAGGAGAGGATTTTCTAGAAGAAGCACTAACCTTCACCACCACACATCTAGTGTCGGCAGCGCTAGCAAACCGTTTAAGCCCCCCGCTTTCGAAACAAGTAATGCATGCCTTGAATCAACCACTCCGGAAGGGCTTACCGAGGCTAGAAGCGAGGCATTACATGTCTCTCCACCAGGAACTACACGGATCACGTAACCAAATTTTGCTGAGTTTTGCAAAGCTGGATTTCAACCTGCTCCAACAACTGCATCAGAAAGAACTAAGTGATATTGCAAGGTGGTGGAAGGAGTTGGACTTTGCAAGCAAGCTACCTTTTGCAAGAGACAGAGTAATTGAGTGCTACTTCTGGATTTTGGGAGTCTACTTTGAGCCTGAGTATTACTTTGCCAGGAGGACACTAACCAAAGTTATTGCCATGACGTCCATTATCGATGACATTTATGATGTGTATGGCACGCTTGAAGAACTAGACCTCTTTACTAGAGCAATTGAGAGGTGGGACATCTCAGCCATGGATGAACTACCGGAGTATATGAAAGTCTGTTACGAGGCACTGTTAGATGTCTATGCTGAAACAGAACAGGAGCTTGCAAGCAAGGGACAATTATATCGTATCAATTACGCAAAAGAAGCGATGAAGAGACAGGTTAGGGCATACGATGCTGAAGCAAAATGGTTTCACAAGAACTACACACCAACAATGGATGAATACATGGAAGTAGCCCTTGTCACTTCAGCCTACTCAATGCTAGCAACCACATCATTTGTTGGAATGGGAGATATCGTTACAAAAGACTCCTTTGAGTGGATCTTCAGTGACCCTAAGATTGTAAAGGCTTCAGCAGTAGTTTGCAGACTCATGGATGACATTGTATCCCACAAGTTTGAGCAAAAGAGAGGACATGTTGCCTCGGCTGTGGAATGTTACATGAAACAATACGGTGCGACAGAAGAAGAAACCATAATTGAATTTCGTAGACAAGTGAGTGATGCATGGAAGGACATAAATGAAGAGTGCCTCCACCCTACACCTCTTGACATGTCCCTCTTAATGCGAGTTCTGAATCTCACACGCGTGATAGATGTAGTATACAAGTCTGAAGATGGCTACACTCACGCTGGAACCATTCTCAAAGATTTTGTAGCTTCTCTACTTATCGAACCTGTGCCTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

557

Amino Acids

64.26

Weight (kDa)

5.14

Isoelectric Point (pI)

51.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 34 - 203 1.1e-51 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 261 - 500 4.5e-106 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 301 - 500 9.1e-64 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 920
Acc36I ACCTGC 1 cut(s) 738
AccB7I CCANNNNNTGG 1 cut(s) 102
AccI GTMKAC 4 cut(s) 73, 858, 1474, 1587
AccII CGCG 1 cut(s) 1572
AccIII TCCGGA 1 cut(s) 621
AciI CCGC 2 cut(s) 209, 581
AclWI GGATC 2 cut(s) 691, 1313
AcsI RAATTY 4 cut(s) 85, 312, 696, 1466
AcuI CTGAAG 6 cut(s) 197, 1182, 1215, 1294, 1320, 1617
AcyI GRCGYC 1 cut(s) 917
AfaI GTAC 1 cut(s) 495
AfeI AGCGCT 1 cut(s) 558
AfiI CCNNNNNNNGG 2 cut(s) 102, 1302
AflIII ACRYGT 5 cut(s) 163, 657, 1399, 1536, 1570
AgsI TTSAA 8 cut(s) 218, 257, 284, 418, 610, 727, 959, 1466
AjnI CCWGG 2 cut(s) 669, 884
AleI CACNNNNGTG 2 cut(s) 357, 1571
AluBI AGCT 5 cut(s) 26, 718, 805, 1084, 1643
AluI AGCT 5 cut(s) 26, 718, 805, 1084, 1643
Alw21I GWGCWC 1 cut(s) 364
Alw26I GTCTC 5 cut(s) 9, 102, 666, 813, 1132
AlwI GGATC 2 cut(s) 691, 1313
AlwNI CAGNNNCTG 1 cut(s) 118
Aor13HI TCCGGA 1 cut(s) 621
Aor51HI AGCGCT 1 cut(s) 558
ApeKI GCWGC 3 cut(s) 307, 385, 554
ApoI RAATTY 4 cut(s) 85, 312, 696, 1466
AspLEI GCGC 1 cut(s) 559
AsuHPI GGTGA 4 cut(s) 167, 344, 356, 523
AsuII TTCGAA 1 cut(s) 587
AsuNHI GCTAGC 2 cut(s) 559, 1246
Bbv12I GWGCWC 1 cut(s) 364
BbvI GCAGC 3 cut(s) 319, 397, 566
BccI CCATC 3 cut(s) 99, 335, 1595
BciT130I CCWGG 2 cut(s) 671, 886
BciVI GTATCC 1 cut(s) 1381
BcoDI GTCTC 5 cut(s) 9, 102, 666, 813, 1132
BfaI CTAG 7 cut(s) 512, 545, 560, 641, 965, 978, 1247
BfoI RGCGCY 1 cut(s) 560
BfuAI ACCTGC 1 cut(s) 738
BfuI GTATCC 1 cut(s) 1381
BisI GCNGC 3 cut(s) 308, 386, 555
BlsI GCNGC 3 cut(s) 309, 387, 556
Bme1390I CCNGG 2 cut(s) 671, 886
BmiI GGNNCC 2 cut(s) 207, 1621
BmrFI CCNGG 2 cut(s) 671, 886
BmsI GCATC 4 cut(s) 52, 754, 1147, 1477
BmtI GCTAGC 2 cut(s) 563, 1250
Bpu14I TTCGAA 1 cut(s) 587
BpuEI CTTGAG 1 cut(s) 15
Bsa29I ATCGAT 1 cut(s) 927
BsaAI YACGTR 1 cut(s) 689
BsaBI GATNNNNATC 1 cut(s) 345
BsaHI GRCGYC 1 cut(s) 917
BsaI GGTCTC 1 cut(s) 102
BsaJI CCNNGG 3 cut(s) 635, 1008, 1407
BsaWI WCCGGW 2 cut(s) 621, 1021
BsaXI ACNNNNNCTCC 2 cut(s) 1266, 1296
Bsc4I CCNNNNNNNGG 2 cut(s) 102, 1302
Bse1I ACTGG 2 cut(s) 112, 212
Bse8I GATNNNNATC 1 cut(s) 345
BseAI TCCGGA 1 cut(s) 621
BseBI CCWGG 2 cut(s) 671, 886
BseCI ATCGAT 1 cut(s) 927
BseDI CCNNGG 3 cut(s) 635, 1008, 1407
BseGI GGATG 4 cut(s) 91, 1018, 1207, 1366
BseJI GATNNNNATC 1 cut(s) 345
BseLI CCNNNNNNNGG 2 cut(s) 102, 1302
BseMII CTCAG 3 cut(s) 695, 861, 1017
BseNI ACTGG 2 cut(s) 112, 212
BseRI GAGGAG 1 cut(s) 513
BseXI GCAGC 3 cut(s) 319, 397, 566
BseYI CCCAGC 1 cut(s) 95
BsgI GTGCAG 1 cut(s) 335
Bsh1236I CGCG 1 cut(s) 1572
BshVI ATCGAT 1 cut(s) 927
BsiHKAI GWGCWC 1 cut(s) 364
BsiSI CCGG 2 cut(s) 622, 1022
BslFI GGGAC 3 cut(s) 1010, 1110, 1525
BslI CCNNNNNNNGG 2 cut(s) 102, 1302
BsmAI GTCTC 5 cut(s) 9, 102, 666, 813, 1132
BsmFI GGGAC 3 cut(s) 1010, 1110, 1525
BsmI GAATGC 1 cut(s) 55
Bso31I GGTCTC 1 cut(s) 102
Bsp119I TTCGAA 1 cut(s) 587
Bsp1286I GDGCHC 1 cut(s) 364
Bsp13I TCCGGA 1 cut(s) 621
Bsp143I GATC 3 cut(s) 346, 683, 1305
Bsp19I CCATGG 1 cut(s) 1008
BspACI CCGC 2 cut(s) 209, 581
BspCNI CTCAG 3 cut(s) 696, 862, 1016
BspDI ATCGAT 1 cut(s) 927
BspEI TCCGGA 1 cut(s) 621
BspFNI CGCG 1 cut(s) 1572
BspHI TCATGA 1 cut(s) 442
BspLI GGNNCC 2 cut(s) 207, 1621
BspMI ACCTGC 1 cut(s) 738
BspOI GCTAGC 2 cut(s) 563, 1250
BspPI GGATC 2 cut(s) 691, 1313
BspQI GCTCTTC 1 cut(s) 369
BspT104I TTCGAA 1 cut(s) 587
BspTNI GGTCTC 1 cut(s) 102
BsrI ACTGG 2 cut(s) 112, 212
BssECI CCNNGG 3 cut(s) 635, 1008, 1407
BssMI GATC 3 cut(s) 346, 683, 1305
BssNAI GTATAC 1 cut(s) 1588
BssNI GRCGYC 1 cut(s) 917
BssT1I CCWWGG 1 cut(s) 1008
Bst1107I GTATAC 1 cut(s) 1588
Bst2UI CCWGG 2 cut(s) 671, 886
Bst4CI ACNGT 4 cut(s) 338, 569, 1053, 1439
Bst6I CTCTTC 4 cut(s) 185, 369, 1130, 1503
BstACI GRCGYC 1 cut(s) 917
BstAPI GCANNNNNTGC 1 cut(s) 1157
BstBAI YACGTR 1 cut(s) 689
BstBI TTCGAA 1 cut(s) 587
BstC8I GCNNGC 8 cut(s) 561, 603, 799, 803, 953, 1086, 1090, 1248
BstDEI CTNAG 6 cut(s) 488, 704, 758, 870, 1003, 1320
BstDSI CCRYGG 1 cut(s) 1008
BstF5I GGATG 4 cut(s) 91, 1018, 1207, 1366
BstFNI CGCG 1 cut(s) 1572
BstH2I RGCGCY 1 cut(s) 560
BstHHI GCGC 1 cut(s) 559
BstKTI GATC 3 cut(s) 349, 686, 1308
BstMAI GTCTC 5 cut(s) 9, 102, 666, 813, 1132
BstMBI GATC 3 cut(s) 346, 683, 1305
BstMWI GCNNNNNNNGC 4 cut(s) 32, 560, 1127, 1157
BstNI CCWGG 2 cut(s) 671, 886
BstNSI RCATGY 5 cut(s) 167, 605, 661, 1403, 1540
BstSCI CCNGG 2 cut(s) 669, 884
BstUI CGCG 1 cut(s) 1572
BstV1I GCAGC 3 cut(s) 319, 397, 566
BstX2I RGATCY 1 cut(s) 1305
BstYI RGATCY 1 cut(s) 1305
BstZ17I GTATAC 1 cut(s) 1588
Bsu15I ATCGAT 1 cut(s) 927
BsuI GTATCC 1 cut(s) 1381
BsuTUI ATCGAT 1 cut(s) 927
BtgI CCRYGG 1 cut(s) 1008
BtgZI GCGATG 2 cut(s) 420, 1145
BtsCI GGATG 4 cut(s) 91, 1018, 1207, 1366
BtsIMutI CAGTG 3 cut(s) 44, 1049, 1318
BveI ACCTGC 1 cut(s) 738
Cac8I GCNNGC 8 cut(s) 561, 603, 799, 803, 953, 1086, 1090, 1248
CaiI CAGNNNCTG 1 cut(s) 118
CciI TCATGA 1 cut(s) 442
CfoI GCGC 1 cut(s) 559
ClaI ATCGAT 1 cut(s) 927
Csp6I GTAC 1 cut(s) 494
CspCI CAANNNNNGTGG 2 cut(s) 1243, 1278
CviQI GTAC 1 cut(s) 494
DdeI CTNAG 6 cut(s) 488, 704, 758, 870, 1003, 1320
DpnI GATC 3 cut(s) 348, 685, 1307
DpnII GATC 3 cut(s) 346, 683, 1305
Eam1104I CTCTTC 4 cut(s) 185, 369, 1130, 1503
EarI CTCTTC 4 cut(s) 185, 369, 1130, 1503
EciI GGCGGA 1 cut(s) 198
Eco130I CCWWGG 1 cut(s) 1008
Eco31I GGTCTC 1 cut(s) 102
Eco32I GATATC 1 cut(s) 1279
Eco47III AGCGCT 1 cut(s) 558
Eco57I CTGAAG 6 cut(s) 197, 1182, 1215, 1294, 1320, 1617
EcoRII CCWGG 2 cut(s) 669, 884
EcoRV GATATC 1 cut(s) 1279
EcoT14I CCWWGG 1 cut(s) 1008
EcoT22I ATGCAT 2 cut(s) 603, 1492
ErhI CCWWGG 1 cut(s) 1008
FalI AAGNNNNNCTT 2 cut(s) 1206, 1238
FaqI GGGAC 3 cut(s) 1010, 1110, 1525
FauI CCCGC 1 cut(s) 588
FblI GTMKAC 4 cut(s) 73, 858, 1474, 1587
Fnu4HI GCNGC 3 cut(s) 308, 386, 555
FokI GGATG 4 cut(s) 78, 1025, 1214, 1373
Fsp4HI GCNGC 3 cut(s) 308, 386, 555
FspBI CTAG 7 cut(s) 512, 545, 560, 641, 965, 978, 1247
GlaI GCGC 1 cut(s) 558
GluI GCNGC 3 cut(s) 308, 386, 555
GsaI CCCAGC 1 cut(s) 99
HaeII RGCGCY 1 cut(s) 560
HapII CCGG 2 cut(s) 622, 1022
HhaI GCGC 1 cut(s) 559
Hin1I GRCGYC 1 cut(s) 917
Hin6I GCGC 1 cut(s) 557
HinP1I GCGC 1 cut(s) 557
HincII GTYRAC 1 cut(s) 74
HindII GTYRAC 1 cut(s) 74
HinfI GANTC 6 cut(s) 253, 610, 855, 1292, 1353, 1561
HpaII CCGG 2 cut(s) 622, 1022
HphI GGTGA 4 cut(s) 167, 344, 356, 523
Hpy166II GTNNAC 4 cut(s) 74, 859, 1475, 1588
Hpy188I TCNGA 3 cut(s) 750, 1560, 1597
Hpy188III TCNNGA 6 cut(s) 317, 443, 512, 622, 844, 1532
Hpy8I GTNNAC 4 cut(s) 74, 859, 1475, 1588
Hpy99I CGWCG 1 cut(s) 78
HpyAV CCTTC 5 cut(s) 172, 538, 619, 775, 1489
HpyCH4III ACNGT 4 cut(s) 338, 569, 1053, 1439
HpyCH4IV ACGT 3 cut(s) 76, 688, 917
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 560, 1127, 1157
HpyF3I CTNAG 6 cut(s) 488, 704, 758, 870, 1003, 1320
HpySE526I ACGT 3 cut(s) 76, 688, 917
Hsp92I GRCGYC 1 cut(s) 917
HspAI GCGC 1 cut(s) 557
Kpn2I TCCGGA 1 cut(s) 621
Kzo9I GATC 3 cut(s) 346, 683, 1305
LguI GCTCTTC 1 cut(s) 369
LmnI GCTCC 3 cut(s) 211, 738, 1081
Lsp1109I GCAGC 3 cut(s) 319, 397, 566
LweI GCATC 4 cut(s) 52, 754, 1147, 1477
MaeI CTAG 7 cut(s) 512, 545, 560, 641, 965, 978, 1247
MaeII ACGT 3 cut(s) 76, 688, 917
MaeIII GTNAC 9 cut(s) 125, 332, 392, 689, 1040, 1225, 1282, 1313, 1421
MalI GATC 3 cut(s) 348, 685, 1307
MboI GATC 3 cut(s) 346, 683, 1305
MfeI CAATTG 1 cut(s) 984
MflI RGATCY 1 cut(s) 1305
MhlI GDGCHC 1 cut(s) 364
MluI ACGCGT 1 cut(s) 1570
MlyI GAGTC 3 cut(s) 864, 1286, 1347
MmeI TCCRAC 5 cut(s) 65, 193, 760, 768, 1246
Mph1103I ATGCAT 2 cut(s) 603, 1492
MroI TCCGGA 1 cut(s) 621
MseI TTAA 3 cut(s) 327, 572, 1547
MslI CAYNNNNRTG 3 cut(s) 357, 447, 1571
MspI CCGG 2 cut(s) 622, 1022
MspR9I CCNGG 2 cut(s) 671, 886
MunI CAATTG 1 cut(s) 984
Mva1269I GAATGC 1 cut(s) 55
MvaI CCWGG 2 cut(s) 671, 886
MvnI CGCG 1 cut(s) 1572
MwoI GCNNNNNNNGC 4 cut(s) 32, 560, 1127, 1157
NcoI CCATGG 1 cut(s) 1008
NdeII GATC 3 cut(s) 346, 683, 1305
NheI GCTAGC 2 cut(s) 559, 1246
NlaIV GGNNCC 2 cut(s) 207, 1621
NmeAIII GCCGAG 1 cut(s) 1388
NmuCI GTSAC 3 cut(s) 332, 1225, 1313
NsiI ATGCAT 2 cut(s) 603, 1492
NspI RCATGY 5 cut(s) 167, 605, 661, 1403, 1540
NspV TTCGAA 1 cut(s) 587
OliI CACNNNNGTG 2 cut(s) 357, 1571
PaeI GCATGC 1 cut(s) 605
PagI TCATGA 1 cut(s) 442
PciI ACATGT 4 cut(s) 163, 657, 1399, 1536
PciSI GCTCTTC 1 cut(s) 369
PctI GAATGC 1 cut(s) 55
PfeI GAWTC 3 cut(s) 253, 610, 1561
PflMI CCANNNNNTGG 1 cut(s) 102
PkrI GCNGC 3 cut(s) 309, 387, 556
PleI GAGTC 3 cut(s) 863, 1286, 1347
PpsI GAGTC 3 cut(s) 863, 1286, 1347
Ppu21I YACGTR 1 cut(s) 689
PscI ACATGT 4 cut(s) 163, 657, 1399, 1536
Psp6I CCWGG 2 cut(s) 669, 884
PspFI CCCAGC 1 cut(s) 95
PspGI CCWGG 2 cut(s) 669, 884
PspN4I GGNNCC 2 cut(s) 207, 1621
PstNI CAGNNNCTG 1 cut(s) 118
PsuI RGATCY 1 cut(s) 1305
RsaI GTAC 1 cut(s) 495
RsaNI GTAC 1 cut(s) 494
RseI CAYNNNNRTG 3 cut(s) 357, 447, 1571
SalI GTCGAC 1 cut(s) 72
SapI GCTCTTC 1 cut(s) 369
SaqAI TTAA 3 cut(s) 327, 572, 1547
SatI GCNGC 3 cut(s) 308, 386, 555
Sau3AI GATC 3 cut(s) 346, 683, 1305
SchI GAGTC 3 cut(s) 864, 1286, 1347
ScrFI CCNGG 2 cut(s) 671, 886
SduI GDGCHC 1 cut(s) 364
SfaNI GCATC 4 cut(s) 52, 754, 1147, 1477
SfuI TTCGAA 1 cut(s) 587
SmiMI CAYNNNNRTG 3 cut(s) 357, 447, 1571
SmlI CTYRAG 1 cut(s) 30
SmoI CTYRAG 1 cut(s) 30
SphI GCATGC 1 cut(s) 605
SsiI CCGC 2 cut(s) 209, 581
SspMI CTAG 7 cut(s) 512, 545, 560, 641, 965, 978, 1247
StyD4I CCNGG 2 cut(s) 669, 884
StyI CCWWGG 1 cut(s) 1008
TaaI ACNGT 4 cut(s) 338, 569, 1053, 1439
TaiI ACGT 3 cut(s) 79, 691, 920
TaqI TCGA 4 cut(s) 73, 587, 927, 1656
TfiI GAWTC 3 cut(s) 253, 610, 1561
Tru1I TTAA 3 cut(s) 327, 572, 1547
Tru9I TTAA 3 cut(s) 327, 572, 1547
TscAI CASTG 3 cut(s) 51, 1056, 1318
TseFI GTSAC 3 cut(s) 332, 1225, 1313
TseI GCWGC 3 cut(s) 307, 385, 554
Tsp45I GTSAC 3 cut(s) 332, 1225, 1313
TspDTI ATGAA 9 cut(s) 393, 431, 489, 1029, 1046, 1148, 1218, 1442, 1521
TspGWI ACGGA 1 cut(s) 696
TspRI CASTG 3 cut(s) 51, 1056, 1318
Van91I CCANNNNNTGG 1 cut(s) 102
XapI RAATTY 4 cut(s) 85, 312, 696, 1466
XbaI TCTAGA 1 cut(s) 511
XceI RCATGY 5 cut(s) 167, 605, 661, 1403, 1540
XmiI GTMKAC 4 cut(s) 73, 858, 1474, 1587
XspI CTAG 7 cut(s) 512, 545, 560, 641, 965, 978, 1247
ZraI GACGTC 1 cut(s) 918
Zsp2I ATGCAT 2 cut(s) 603, 1492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.