FvH4_3g21560

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000053
Physical Location & Seq
Reverse (-)
14654572 .. 14657421
2850 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g21560.t1

Sequence Viewer

Length: 1539 bp
ATGTCTTTCCAAGCAGCTATTGTGACACCAAATGCTAAGCCTGAAATTGTTCACAGGACGGCAAATTATCACCCTAGTATTTGGGGAGATCGTTTCATCAAATGCGATTCCGAAGACAACATAACTCGTGTCCTTAGGCAACAACAAGTTGAGGAGCTGAAAGCAGTAGTAAAGAGAGAAGTATTCAAAAATCAGGCAACTGATTTTTCACATCAACTGAAGTTAATCGATGCGATCCAGCAGCATGGATACAATGTTTCATGTGATATATTCAACAAGTTCAAGGATGCAAATGGTAGCTTCAACGAATGCTTAATCGATGATGTGCCGGGTATGCTAAGCTTGTATGAAGCAGCACATCTTAGGGTGCGGGGAGAAGAGATACTAGATGAGGCTGTTGTTTTCACCACCACTAACCTTGAGAAAGTAACAACTGGTGTAAGCTACCCACTGGCAGCGCAAGTAACTCAGGCCTTACATAGACCTCTCCGGAAAGATCTCGAGAGGGTTTGTGCTAGGCGTTACATGTCAATATACCAAAACGAAGCTTCACACAATGAAGCTTTGCTGAAACTTGCTAAGTTGGATTTCAATCTTGTTCAGTCTTTGCACAAAGAGGAGCTCAGTGAGATCACCAAGTGGTGGAAGGAGCTAGACTTTGCAAGGAAGCTGTCATTTGCAAGAGATAGGATGGTGGAGTTGTACTTCTGGATAGTGGGAGTTTACTTTGAACCCCACTACTCTTCTGCTCGAAAAATTCTTACAAAAGTGATTGCGCTGGTATCAGTAATGGATGATATCTACGATGCATTTGCTACCTTTGAAGAATTGGATATCTTTACTGCAGCTATGGAGAGGTGGGATATCAAATGCATGGATGACCTCCCAGACTATATGCAAATATTTTATAGTGCACTTTTGAATGTGGTCAGTGAAATTGAGGAAGAATTGGCAAAGGAAGGAAAATCATACCGAGTTTATTATGTTAAGGAATCATTGAAAGGTGTGGCTCGAGCATACTTTGAAGAGGCCAAATGGTTCAAAGACGAGCGAATCCCAACCGTGGAGGAGTATTTACGTTCTGCAATATTTTCAACTGGTTATCCTATGCTTGCAACTGTATCCTTGGTTGGCATGGGGGATATTGTAACAGAGGAGACATTTCAATGGCTGTTCAATGATCCTAAAATCGTCATAGCTTCCACAACACTTTTTCGACTCATGGACGACATTGTCACCACCAAGTTTGAGAAAGAGCGAGGTCATGTTGCTTCGAGTATCGATTGCTACATGAAACAACATGGGGTCTCAGAGGAAGAGGTGATCGAGGTGTTTAACAAGCAGGTTGTGGATTACTGGAAGGATATAAACGAAGAGTTTCTAAGGCCAACTGCTGTTCCAATGCCTGTGCTTCTGCGTGTTCTTAACTTGACAAAAGTAGTTGATCTGCTTTACAAAGGAGAAGATGGCTACACTCGTGTTGGAAAAGTTATGAAAGAATGTGTTGCTACACATTTTATTGATCCTGTGCCACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

513

Amino Acids

59.24

Weight (kDa)

5.27

Isoelectric Point (pI)

40.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 80 - 159 1.1e-27 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 216 - 455 2.1e-99 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 256 - 455 9e-56 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1232
Acc36I ACCTGC 1 cut(s) 1333
AccB7I CCANNNNNTGG 1 cut(s) 642
AccIII TCCGGA 1 cut(s) 489
AciI CCGC 1 cut(s) 370
AclWI GGATC 3 cut(s) 229, 1175, 1517
AcsI RAATTY 1 cut(s) 756
AcuI CTGAAG 1 cut(s) 239
AdeI CACNNNGTG 1 cut(s) 639
AfaI GTAC 1 cut(s) 704
AfiI CCNNNNNNNGG 2 cut(s) 642, 1063
AflIII ACRYGT 1 cut(s) 525
Alw21I GWGCWC 2 cut(s) 624, 916
Alw26I GTCTC 2 cut(s) 1151, 1312
Alw44I GTGCAC 1 cut(s) 912
AlwI GGATC 3 cut(s) 229, 1175, 1517
Ama87I CYCGRG 2 cut(s) 500, 1011
Aor13HI TCCGGA 1 cut(s) 489
AoxI GGCC 3 cut(s) 471, 1029, 1385
ApaLI GTGCAC 1 cut(s) 912
ApeKI GCWGC 5 cut(s) 14, 241, 353, 455, 845
ApoI RAATTY 1 cut(s) 756
Asp700I GAANNNNTTC 2 cut(s) 48, 1377
AspLEI GCGC 2 cut(s) 460, 778
AsuC2I CCSGG 1 cut(s) 330
AsuHPI GGTGA 5 cut(s) 62, 397, 625, 1228, 1333
AvaI CYCGRG 2 cut(s) 500, 1011
AxyI CCTNAGG 1 cut(s) 134
BaeGI GKGCMC 1 cut(s) 916
BanII GRGCYC 1 cut(s) 624
BauI CACGAG 2 cut(s) 126, 1476
BbsI GAAGAC 1 cut(s) 120
Bbv12I GWGCWC 2 cut(s) 624, 916
BbvI GCAGC 5 cut(s) 26, 253, 365, 467, 857
BccI CCATC 2 cut(s) 685, 1460
BceAI ACGGC 1 cut(s) 75
BcgI CGANNNNNNTGC 2 cut(s) 794, 828
BciVI GTATCC 2 cut(s) 242, 1132
BcnI CCSGG 1 cut(s) 330
BcoDI GTCTC 2 cut(s) 1151, 1312
BfaI CTAG 4 cut(s) 75, 386, 516, 653
BfmI CTRYAG 1 cut(s) 843
BfuAI ACCTGC 1 cut(s) 1333
BfuI GTATCC 2 cut(s) 242, 1132
BglII AGATCT 1 cut(s) 496
BisI GCNGC 5 cut(s) 15, 242, 354, 456, 846
BlpI GCTNAGC 2 cut(s) 36, 338
BlsI GCNGC 5 cut(s) 16, 243, 355, 457, 847
Bme1390I CCNGG 1 cut(s) 330
BmeT110I CYCGRG 2 cut(s) 500, 1011
BmrFI CCNGG 1 cut(s) 330
BmsI GCATC 3 cut(s) 220, 277, 796
BpiI GAAGAC 1 cut(s) 120
Bpu1102I GCTNAGC 2 cut(s) 36, 338
BpuEI CTTGAG 1 cut(s) 440
BpuMI CCSGG 1 cut(s) 330
Bsa29I ATCGAT 3 cut(s) 228, 318, 1281
BsaBI GATNNNNATC 1 cut(s) 591
BsaI GGTCTC 1 cut(s) 1312
BsaJI CCNNGG 2 cut(s) 1062, 1125
BsaWI WCCGGW 1 cut(s) 489
Bsc4I CCNNNNNNNGG 2 cut(s) 642, 1063
Bse1I ACTGG 4 cut(s) 439, 456, 1102, 1361
Bse21I CCTNAGG 1 cut(s) 134
Bse8I GATNNNNATC 1 cut(s) 591
BseAI TCCGGA 1 cut(s) 489
BseCI ATCGAT 3 cut(s) 228, 318, 1281
BseDI CCNNGG 2 cut(s) 1062, 1125
BseGI GGATG 4 cut(s) 292, 696, 799, 883
BseJI GATNNNNATC 1 cut(s) 591
BseLI CCNNNNNNNGG 2 cut(s) 642, 1063
BseMII CTCAG 3 cut(s) 482, 637, 1323
BseNI ACTGG 4 cut(s) 439, 456, 1102, 1361
BseRI GAGGAG 4 cut(s) 167, 632, 1082, 1169
BseSI GKGCMC 1 cut(s) 916
BseXI GCAGC 5 cut(s) 26, 253, 365, 467, 857
BshFI GGCC 3 cut(s) 473, 1031, 1387
BshVI ATCGAT 3 cut(s) 228, 318, 1281
BsiHKAI GWGCWC 2 cut(s) 624, 916
BsiHKCI CYCGRG 2 cut(s) 500, 1011
BsiSI CCGG 2 cut(s) 329, 490
BslI CCNNNNNNNGG 2 cut(s) 642, 1063
BsmAI GTCTC 2 cut(s) 1151, 1312
BsmI GAATGC 1 cut(s) 314
BsnI GGCC 3 cut(s) 473, 1031, 1387
Bso31I GGTCTC 1 cut(s) 1312
BsoBI CYCGRG 2 cut(s) 500, 1011
Bsp1286I GDGCHC 2 cut(s) 624, 916
Bsp13I TCCGGA 1 cut(s) 489
Bsp143I GATC 8 cut(s) 88, 234, 496, 630, 1180, 1323, 1444, 1522
Bsp1720I GCTNAGC 2 cut(s) 36, 338
BspACI CCGC 1 cut(s) 370
BspANI GGCC 3 cut(s) 473, 1031, 1387
BspCNI CTCAG 3 cut(s) 481, 636, 1322
BspDI ATCGAT 3 cut(s) 228, 318, 1281
BspEI TCCGGA 1 cut(s) 489
BspMAI CTGCAG 1 cut(s) 847
BspMI ACCTGC 1 cut(s) 1333
BspPI GGATC 3 cut(s) 229, 1175, 1517
BspTNI GGTCTC 1 cut(s) 1312
BsrI ACTGG 4 cut(s) 439, 456, 1102, 1361
BssECI CCNNGG 2 cut(s) 1062, 1125
BssMI GATC 8 cut(s) 88, 234, 496, 630, 1180, 1323, 1444, 1522
BssSI CACGAG 2 cut(s) 126, 1476
BssT1I CCWWGG 1 cut(s) 1125
Bst2BI CACGAG 2 cut(s) 126, 1476
Bst4CI ACNGT 2 cut(s) 1063, 1120
Bst6I CTCTTC 5 cut(s) 372, 748, 1020, 1311, 1368
BstC8I GCNNGC 1 cut(s) 1113
BstDEI CTNAG 9 cut(s) 36, 134, 338, 362, 468, 579, 623, 1309, 1382
BstDSI CCRYGG 1 cut(s) 1062
BstF5I GGATG 4 cut(s) 292, 696, 799, 883
BstHHI GCGC 2 cut(s) 460, 778
BstKTI GATC 8 cut(s) 91, 237, 499, 633, 1183, 1326, 1447, 1525
BstMAI GTCTC 2 cut(s) 1151, 1312
BstMBI GATC 8 cut(s) 88, 234, 496, 630, 1180, 1323, 1444, 1522
BstMWI GCNNNNNNNGC 1 cut(s) 334
BstNSI RCATGY 1 cut(s) 529
BstSCI CCNGG 1 cut(s) 328
BstSFI CTRYAG 1 cut(s) 843
BstSLI GKGCMC 1 cut(s) 916
BstV1I GCAGC 5 cut(s) 26, 253, 365, 467, 857
BstV2I GAAGAC 1 cut(s) 120
BstX2I RGATCY 1 cut(s) 496
BstXI CCANNNNNNTGG 1 cut(s) 245
BstYI RGATCY 1 cut(s) 496
Bsu15I ATCGAT 3 cut(s) 228, 318, 1281
Bsu36I CCTNAGG 1 cut(s) 134
BsuI GTATCC 2 cut(s) 242, 1132
BsuRI GGCC 3 cut(s) 473, 1031, 1387
BsuTUI ATCGAT 3 cut(s) 228, 318, 1281
BtgI CCRYGG 1 cut(s) 1062
BtsCI GGATG 4 cut(s) 292, 696, 799, 883
BtsIMutI CAGTG 3 cut(s) 449, 631, 937
BveI ACCTGC 1 cut(s) 1333
Cac8I GCNNGC 1 cut(s) 1113
CfoI GCGC 2 cut(s) 460, 778
ClaI ATCGAT 3 cut(s) 228, 318, 1281
Csp6I GTAC 1 cut(s) 703
CspCI CAANNNNNGTGG 4 cut(s) 400, 435, 1228, 1263
CviAII CATG 9 cut(s) 245, 261, 526, 874, 1135, 1222, 1265, 1291, 1301
CviQI GTAC 1 cut(s) 703
DdeI CTNAG 9 cut(s) 36, 134, 338, 362, 468, 579, 623, 1309, 1382
DpnI GATC 8 cut(s) 90, 236, 498, 632, 1182, 1325, 1446, 1524
DpnII GATC 8 cut(s) 88, 234, 496, 630, 1180, 1323, 1444, 1522
DraIII CACNNNGTG 1 cut(s) 639
DrdI GACNNNNNNGTC 1 cut(s) 1232
DseDI GACNNNNNNGTC 1 cut(s) 1232
Eam1104I CTCTTC 5 cut(s) 372, 748, 1020, 1311, 1368
EarI CTCTTC 5 cut(s) 372, 748, 1020, 1311, 1368
Ecl136II GAGCTC 1 cut(s) 622
Eco130I CCWWGG 1 cut(s) 1125
Eco147I AGGCCT 1 cut(s) 473
Eco24I GRGCYC 1 cut(s) 624
Eco31I GGTCTC 1 cut(s) 1312
Eco32I GATATC 3 cut(s) 799, 835, 865
Eco53kI GAGCTC 1 cut(s) 622
Eco57I CTGAAG 1 cut(s) 239
Eco81I CCTNAGG 1 cut(s) 134
Eco88I CYCGRG 2 cut(s) 500, 1011
EcoICRI GAGCTC 1 cut(s) 622
EcoRV GATATC 3 cut(s) 799, 835, 865
EcoT14I CCWWGG 1 cut(s) 1125
EcoT22I ATGCAT 2 cut(s) 811, 875
EcoT38I GRGCYC 1 cut(s) 624
ErhI CCWWGG 1 cut(s) 1125
FaeI CATG 9 cut(s) 248, 264, 529, 877, 1138, 1225, 1268, 1294, 1304
FatI CATG 9 cut(s) 244, 260, 525, 873, 1134, 1221, 1264, 1290, 1300
FauI CCCGC 1 cut(s) 363
Fnu4HI GCNGC 5 cut(s) 15, 242, 354, 456, 846
FokI GGATG 4 cut(s) 299, 703, 806, 890
FriOI GRGCYC 1 cut(s) 624
Fsp4HI GCNGC 5 cut(s) 15, 242, 354, 456, 846
FspBI CTAG 4 cut(s) 75, 386, 516, 653
GlaI GCGC 2 cut(s) 459, 777
GluI GCNGC 5 cut(s) 15, 242, 354, 456, 846
HaeIII GGCC 3 cut(s) 473, 1031, 1387
HapII CCGG 2 cut(s) 329, 490
HhaI GCGC 2 cut(s) 460, 778
Hin1II CATG 9 cut(s) 248, 264, 529, 877, 1138, 1225, 1268, 1294, 1304
Hin6I GCGC 2 cut(s) 458, 776
HinP1I GCGC 2 cut(s) 458, 776
HindIII AAGCTT 3 cut(s) 340, 546, 561
HinfI GANTC 4 cut(s) 107, 992, 1053, 1218
HpaII CCGG 2 cut(s) 329, 490
HphI GGTGA 5 cut(s) 62, 397, 625, 1228, 1333
Hpy166II GTNNAC 3 cut(s) 52, 724, 914
Hpy188I TCNGA 2 cut(s) 112, 1312
Hpy188III TCNNGA 4 cut(s) 490, 500, 502, 709
Hpy8I GTNNAC 3 cut(s) 52, 724, 914
HpyAV CCTTC 3 cut(s) 640, 953, 1354
HpyCH4III ACNGT 2 cut(s) 1063, 1120
HpyCH4IV ACGT 1 cut(s) 1078
HpyF10VI GCNNNNNNNGC 1 cut(s) 334
HpyF3I CTNAG 9 cut(s) 36, 134, 338, 362, 468, 579, 623, 1309, 1382
HpySE526I ACGT 1 cut(s) 1078
Hsp92II CATG 9 cut(s) 248, 264, 529, 877, 1138, 1225, 1268, 1294, 1304
HspAI GCGC 2 cut(s) 458, 776
Kpn2I TCCGGA 1 cut(s) 489
Kzo9I GATC 8 cut(s) 88, 234, 496, 630, 1180, 1323, 1444, 1522
LmnI GCTCC 3 cut(s) 154, 619, 649
Lsp1109I GCAGC 5 cut(s) 26, 253, 365, 467, 857
LweI GCATC 3 cut(s) 220, 277, 796
MaeI CTAG 4 cut(s) 75, 386, 516, 653
MaeII ACGT 1 cut(s) 1078
MaeIII GTNAC 6 cut(s) 22, 427, 463, 521, 1147, 1234
MalI GATC 8 cut(s) 90, 236, 498, 632, 1182, 1325, 1446, 1524
MboI GATC 8 cut(s) 88, 234, 496, 630, 1180, 1323, 1444, 1522
MboII GAAGA 9 cut(s) 125, 389, 735, 836, 956, 1037, 1328, 1385, 1475
MflI RGATCY 1 cut(s) 496
MhlI GDGCHC 2 cut(s) 624, 916
MluCI AATT 6 cut(s) 45, 64, 756, 827, 936, 947
MlyI GAGTC 1 cut(s) 1212
MmeI TCCRAC 2 cut(s) 564, 1462
Mph1103I ATGCAT 2 cut(s) 811, 875
MroI TCCGGA 1 cut(s) 489
MroXI GAANNNNTTC 2 cut(s) 48, 1377
MseI TTAA 5 cut(s) 224, 314, 987, 1335, 1425
MslI CAYNNNNRTG 1 cut(s) 1165
MspI CCGG 2 cut(s) 329, 490
MspR9I CCNGG 1 cut(s) 330
Mva1269I GAATGC 1 cut(s) 314
MwoI GCNNNNNNNGC 1 cut(s) 334
NciI CCSGG 1 cut(s) 330
NdeII GATC 8 cut(s) 88, 234, 496, 630, 1180, 1323, 1444, 1522
NlaIII CATG 9 cut(s) 248, 264, 529, 877, 1138, 1225, 1268, 1294, 1304
NmuCI GTSAC 2 cut(s) 22, 1234
NsiI ATGCAT 2 cut(s) 811, 875
NspI RCATGY 1 cut(s) 529
PaeR7I CTCGAG 2 cut(s) 500, 1011
PceI AGGCCT 1 cut(s) 473
PciI ACATGT 1 cut(s) 525
PctI GAATGC 1 cut(s) 314
PdmI GAANNNNTTC 2 cut(s) 48, 1377
PfeI GAWTC 3 cut(s) 107, 992, 1053
PflFI GACNNNGTC 1 cut(s) 1232
PflMI CCANNNNNTGG 1 cut(s) 642
PkrI GCNGC 5 cut(s) 16, 243, 355, 457, 847
PleI GAGTC 1 cut(s) 1212
PpsI GAGTC 1 cut(s) 1212
PscI ACATGT 1 cut(s) 525
Psp124BI GAGCTC 1 cut(s) 624
PspXI VCTCGAGB 1 cut(s) 1011
PstI CTGCAG 1 cut(s) 847
PsuI RGATCY 1 cut(s) 496
PsyI GACNNNGTC 1 cut(s) 1232
RsaI GTAC 1 cut(s) 704
RsaNI GTAC 1 cut(s) 703
RseI CAYNNNNRTG 1 cut(s) 1165
SacI GAGCTC 1 cut(s) 624
SaqAI TTAA 5 cut(s) 224, 314, 987, 1335, 1425
SatI GCNGC 5 cut(s) 15, 242, 354, 456, 846
Sau3AI GATC 8 cut(s) 88, 234, 496, 630, 1180, 1323, 1444, 1522
SchI GAGTC 1 cut(s) 1212
ScrFI CCNGG 1 cut(s) 330
SduI GDGCHC 2 cut(s) 624, 916
SfaNI GCATC 3 cut(s) 220, 277, 796
SfcI CTRYAG 1 cut(s) 843
Sfr274I CTCGAG 2 cut(s) 500, 1011
SlaI CTCGAG 2 cut(s) 500, 1011
SmiMI CAYNNNNRTG 1 cut(s) 1165
SmlI CTYRAG 3 cut(s) 419, 500, 1011
SmoI CTYRAG 3 cut(s) 419, 500, 1011
Sse9I AATT 6 cut(s) 45, 64, 756, 827, 936, 947
SseBI AGGCCT 1 cut(s) 473
SsiI CCGC 1 cut(s) 370
SspI AATATT 2 cut(s) 903, 1089
SspMI CTAG 4 cut(s) 75, 386, 516, 653
SstI GAGCTC 1 cut(s) 624
StuI AGGCCT 1 cut(s) 473
StyD4I CCNGG 1 cut(s) 328
StyI CCWWGG 1 cut(s) 1125
TaaI ACNGT 2 cut(s) 1063, 1120
TaiI ACGT 1 cut(s) 1081
TaqI TCGA 9 cut(s) 228, 318, 501, 751, 1012, 1216, 1274, 1281, 1326
TasI AATT 6 cut(s) 45, 64, 756, 827, 936, 947
TatI WGTACW 1 cut(s) 702
TfiI GAWTC 3 cut(s) 107, 992, 1053
Tru1I TTAA 5 cut(s) 224, 314, 987, 1335, 1425
Tru9I TTAA 5 cut(s) 224, 314, 987, 1335, 1425
TscAI CASTG 3 cut(s) 456, 631, 937
TseFI GTSAC 2 cut(s) 22, 1234
TseI GCWGC 5 cut(s) 14, 241, 353, 455, 845
Tsp45I GTSAC 2 cut(s) 22, 1234
TspDTI ATGAA 6 cut(s) 85, 249, 363, 573, 1307, 1508
TspRI CASTG 3 cut(s) 456, 631, 937
Tth111I GACNNNGTC 1 cut(s) 1232
Van91I CCANNNNNTGG 1 cut(s) 642
VneI GTGCAC 1 cut(s) 912
XapI RAATTY 1 cut(s) 756
XceI RCATGY 1 cut(s) 529
XhoI CTCGAG 2 cut(s) 500, 1011
XmnI GAANNNNTTC 2 cut(s) 48, 1377
XspI CTAG 4 cut(s) 75, 386, 516, 653
Zsp2I ATGCAT 2 cut(s) 811, 875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.