MD11G1232700.v1.1

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000053
Physical Location & Seq
Reverse (-)
33917616 .. 33920150
2535 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1232700.v1.1.491

Sequence Viewer

Length: 1737 bp
ATGATCGTCCACGCATTATTCTTCATACTGACCAAAATTCTCCGACAGGAAAGCATGGCATTAATCCAGGATTCAGCAGAAGCTCAATCACAAAATGCGAAGCCTGAAGTTGCTCGGCGGACGGCAAATTTTCACCCAAGCGTTTGGGGAGACCAGTTCATAAATTATGATGATTCCCAAGACATTATTACTCAAGCTCAAAGTCAACAACAGGTTGATGAACTCAAAGAAATTGTGAGGGAAGCATTCACAACTAGTGCAACTGATTTTTCACTTCAGCTAAAGTTGATTGATGCAATTCAGCGCCTTGGCTTGGCATACCATTTTGAAAGAGAAATCGGAGAAGCACTTGAACGTATGCATTCTACGTTTGATGATGACCATGGCAACGATGATGTTGATCTATACACTGTTGCTCTTGGTTTCCGGCTGCTAAGACAACATGGATATAATGTTTCATGTGCCATATTCAACAATTTCAAAGATGAAAGTAGTAACTTCAAGGAAAGCTTGATTGATGACATGTCTGGTATGCTAAGCTTTTATGAAGCAACACATCTGAGGGTGCATGGAGAAGACATATTAGAAAAGGCTCTTGTATTCACCACCACTCACCTTGAGTCAGCAGCAACCCGTGTAAGCAATCCACAGGCTACACAAATTACTCAAGCCCTAGAGAGACCTCTGCGAAAAAGTCTAGAGAGATTATCTGCACGACGTTACATTTCGATCTACCAAGAAGAAGCTTCACATAATGAATCTCTTCTGAAACTTGCAAAGTTAGACTTCAATCTTGTCCAGCCTTTGCACAAAAAGGAGCTCCAAGAGATCACTAGGTGGTGGAGAGCACTAGACTTTGAAAGGAAGCTGCCATTTGCAAGAGATAGGATGGTAGAGTTGTACTTTTGGATCGTCGGAGTGTATTTTGAACCCCAGTACTCTGTTGGGAGAAAAATTATGACACAAGTGAGTGTCTTGCTGACAATATTGGATGATATCTACGACGCATTTGGTACATTTGAAGAGCTCGTAATCTTTACTGAAGCGATTGACAGGTGGGATGTCAATTGCATTGATGAACTCCCGGAATATATGCAAACATTTTATCTTGCACTTTTAAATCTCTACAATGATATTGAGGCAGAGATGGCGAAGGAAGGAAGATCATACCGAGTTCCATATGCAATACAAGCTATGAAAGATCAAGCTCGATCCTATTTTAATGAGGCCCGATGGTTGCACGAGGGACGAGTTCCAAGCATGGAGGAGTATATGCGTGTTGCAACAGTTTCAATTAGTTACACCTTTCTTACAACCATATCTTTACTTGGCATGGGAGATGTTGTGACAAAGGAAGCATTTGAGTGGTTGTTCACTGACCCCAAAATTGTTAGAGCTGCAAATACCATTTTTAGGCTCATGGATGACATTGTTTCCACAGACTTTGAGAAAGAGAGAGGGCATGCTGCTTCTAGTGTTGACTGCTACATGAAACAATATGGGGTGTCAGTGCAAGAGACGGTTGATGTTTTTCAGAAGCAAATTATGGATTTGTGGAAGGATATAAACGAGGAGTTCCTTAGACCAACTTTTGTGCCAATGTCTGTTCTTATGCGCGTTCTCAATTTAACAAGAGTAACTGATCTTCTTTACAAAGGGGAAGATGGATTCACACGTGTGGGAAAGGTGACGAGAGATAGTATTACTTCAATCTGTATCGATCCAGTGCCATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

579

Amino Acids

67.0

Weight (kDa)

4.97

Isoelectric Point (pI)

40.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 48 - 225 7.6e-58 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 284 - 521 4.6e-98 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 323 - 521 3.9e-60 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1617
AciI CCGC 1 cut(s) 118
AclWI GGATC 3 cut(s) 917, 1206, 1715
AcsI RAATTY 2 cut(s) 36, 127
AcuI CTGAAG 3 cut(s) 126, 260, 1062
AcvI CACGTG 1 cut(s) 1676
AdeI CACNNNGTG 1 cut(s) 837
AfaI GTAC 3 cut(s) 902, 938, 1015
AfiI CCNNNNNNNGG 2 cut(s) 313, 1413
AflIII ACRYGT 3 cut(s) 522, 1673, 1675
AhlI ACTAGT 1 cut(s) 254
AjnI CCWGG 1 cut(s) 66
AleI CACNNNNGTG 1 cut(s) 1676
Alw21I GWGCWC 3 cut(s) 822, 850, 1029
Alw26I GTCTC 3 cut(s) 144, 673, 1511
AlwI GGATC 3 cut(s) 917, 1206, 1715
AoxI GGCC 1 cut(s) 1227
ApeKI GCWGC 5 cut(s) 430, 626, 868, 1397, 1466
ApoI RAATTY 2 cut(s) 36, 127
AseI ATTAAT 1 cut(s) 62
Asp700I GAANNNNTTC 1 cut(s) 762
AspLEI GCGC 2 cut(s) 306, 1617
AspS9I GGNCC 1 cut(s) 1228
AsuC2I CCSGG 1 cut(s) 1085
AsuHPI GGTGA 4 cut(s) 125, 595, 605, 1699
BanII GRGCYC 2 cut(s) 822, 1029
BarI GAAGNNNNNNTAC 2 cut(s) 1629, 1661
BauI CACGAG 1 cut(s) 1241
BbrPI CACGTG 1 cut(s) 1676
BbsI GAAGAC 1 cut(s) 582
Bbv12I GWGCWC 3 cut(s) 822, 850, 1029
BbvI GCAGC 5 cut(s) 417, 638, 855, 1384, 1453
BccI CCATC 4 cut(s) 883, 1141, 1227, 1658
BceAI ACGGC 1 cut(s) 138
BciT130I CCWGG 1 cut(s) 68
BcnI CCSGG 1 cut(s) 1085
BcoDI GTCTC 3 cut(s) 144, 673, 1511
BcuI ACTAGT 1 cut(s) 254
BfaI CTAG 6 cut(s) 255, 674, 698, 834, 851, 1473
BfoI RGCGCY 1 cut(s) 307
BisI GCNGC 5 cut(s) 431, 627, 869, 1398, 1467
BlpI GCTNAGC 1 cut(s) 536
BlsI GCNGC 5 cut(s) 432, 628, 870, 1399, 1468
BmcAI AGTACT 1 cut(s) 938
Bme1390I CCNGG 2 cut(s) 68, 1085
BmgT120I GGNCC 1 cut(s) 1228
BmrFI CCNGG 2 cut(s) 68, 1085
BmrI ACTGGG 1 cut(s) 928
BmsI GCATC 1 cut(s) 283
BmuI ACTGGG 1 cut(s) 928
BpiI GAAGAC 1 cut(s) 582
Bpu1102I GCTNAGC 1 cut(s) 536
BpuEI CTTGAG 3 cut(s) 177, 638, 651
BpuMI CCSGG 1 cut(s) 1085
Bsa29I ATCGAT 1 cut(s) 1719
BsaAI YACGTR 1 cut(s) 1676
BsaBI GATNNNNATC 1 cut(s) 399
BsaI GGTCTC 2 cut(s) 144, 673
BsaJI CCNNGG 2 cut(s) 307, 382
BsaXI ACNNNNNCTCC 2 cut(s) 1329, 1359
Bsc4I CCNNNNNNNGG 2 cut(s) 313, 1413
Bse1I ACTGG 3 cut(s) 154, 934, 1724
Bse8I GATNNNNATC 1 cut(s) 399
BseBI CCWGG 1 cut(s) 68
BseCI ATCGAT 1 cut(s) 1719
BseDI CCNNGG 2 cut(s) 307, 382
BseGI GGATG 4 cut(s) 894, 997, 1066, 1429
BseJI GATNNNNATC 1 cut(s) 399
BseLI CCNNNNNNNGG 2 cut(s) 313, 1413
BseMII CTCAG 1 cut(s) 551
BseNI ACTGG 3 cut(s) 154, 934, 1724
BseRI GAGGAG 2 cut(s) 1280, 1586
BseXI GCAGC 5 cut(s) 417, 638, 855, 1384, 1453
BsgI GTGCAG 1 cut(s) 696
Bsh1236I CGCG 1 cut(s) 1617
BshFI GGCC 1 cut(s) 1229
BshVI ATCGAT 1 cut(s) 1719
BsiHKAI GWGCWC 3 cut(s) 822, 850, 1029
BsiSI CCGG 2 cut(s) 427, 1085
BslFI GGGAC 1 cut(s) 1260
BslI CCNNNNNNNGG 2 cut(s) 313, 1413
BsmAI GTCTC 3 cut(s) 144, 673, 1511
BsmBI CGTCTC 1 cut(s) 1511
BsmFI GGGAC 1 cut(s) 1260
BsmI GAATGC 2 cut(s) 245, 361
BsnI GGCC 1 cut(s) 1229
Bso31I GGTCTC 2 cut(s) 144, 673
Bsp1286I GDGCHC 3 cut(s) 822, 850, 1029
Bsp1720I GCTNAGC 1 cut(s) 536
Bsp19I CCATGG 1 cut(s) 382
BspACI CCGC 1 cut(s) 118
BspANI GGCC 1 cut(s) 1229
BspCNI CTCAG 1 cut(s) 552
BspDI ATCGAT 1 cut(s) 1719
BspFNI CGCG 1 cut(s) 1617
BspPI GGATC 3 cut(s) 917, 1206, 1715
BspQI GCTCTTC 1 cut(s) 1017
BspTNI GGTCTC 2 cut(s) 144, 673
BsrI ACTGG 3 cut(s) 154, 934, 1724
BssECI CCNNGG 2 cut(s) 307, 382
BssSI CACGAG 1 cut(s) 1241
BssT1I CCWWGG 2 cut(s) 307, 382
Bst2BI CACGAG 1 cut(s) 1241
Bst2UI CCWGG 1 cut(s) 68
Bst4CI ACNGT 3 cut(s) 412, 1288, 1522
Bst6I CTCTTC 2 cut(s) 768, 1017
BstBAI YACGTR 1 cut(s) 1676
BstC8I GCNNGC 1 cut(s) 1464
BstDEI CTNAG 4 cut(s) 434, 536, 560, 1580
BstDSI CCRYGG 1 cut(s) 382
BstF5I GGATG 4 cut(s) 894, 997, 1066, 1429
BstFNI CGCG 1 cut(s) 1617
BstH2I RGCGCY 1 cut(s) 307
BstHHI GCGC 2 cut(s) 306, 1617
BstMAI GTCTC 3 cut(s) 144, 673, 1511
BstMWI GCNNNNNNNGC 2 cut(s) 1148, 1190
BstNI CCWGG 1 cut(s) 68
BstNSI RCATGY 2 cut(s) 526, 1466
BstSCI CCNGG 2 cut(s) 66, 1083
BstUI CGCG 1 cut(s) 1617
BstV1I GCAGC 5 cut(s) 417, 638, 855, 1384, 1453
BstV2I GAAGAC 1 cut(s) 582
BstXI CCANNNNNNTGG 1 cut(s) 144
Bsu15I ATCGAT 1 cut(s) 1719
BsuRI GGCC 1 cut(s) 1229
BsuTUI ATCGAT 1 cut(s) 1719
BtgI CCRYGG 1 cut(s) 382
BtsCI GGATG 4 cut(s) 894, 997, 1066, 1429
BtsIMutI CAGTG 4 cut(s) 408, 1374, 1515, 1731
Cac8I GCNNGC 1 cut(s) 1464
CfoI GCGC 2 cut(s) 306, 1617
Cfr13I GGNCC 1 cut(s) 1228
ClaI ATCGAT 1 cut(s) 1719
CseI GACGC 1 cut(s) 1013
Csp6I GTAC 3 cut(s) 901, 937, 1014
CspCI CAANNNNNGTGG 4 cut(s) 598, 633, 1426, 1461
CviQI GTAC 3 cut(s) 901, 937, 1014
DdeI CTNAG 4 cut(s) 434, 536, 560, 1580
DraI TTTAAA 1 cut(s) 1119
DraIII CACNNNGTG 1 cut(s) 837
Eam1104I CTCTTC 2 cut(s) 768, 1017
EarI CTCTTC 2 cut(s) 768, 1017
EciI GGCGGA 1 cut(s) 133
Ecl136II GAGCTC 2 cut(s) 820, 1027
Eco130I CCWWGG 2 cut(s) 307, 382
Eco24I GRGCYC 2 cut(s) 822, 1029
Eco31I GGTCTC 2 cut(s) 144, 673
Eco32I GATATC 1 cut(s) 997
Eco53kI GAGCTC 2 cut(s) 820, 1027
Eco57I CTGAAG 3 cut(s) 126, 260, 1062
Eco72I CACGTG 1 cut(s) 1676
EcoICRI GAGCTC 2 cut(s) 820, 1027
EcoRII CCWGG 1 cut(s) 66
EcoRV GATATC 1 cut(s) 997
EcoT14I CCWWGG 2 cut(s) 307, 382
EcoT22I ATGCAT 1 cut(s) 363
EcoT38I GRGCYC 2 cut(s) 822, 1029
ErhI CCWWGG 2 cut(s) 307, 382
Esp3I CGTCTC 1 cut(s) 1511
FalI AAGNNNNNCTT 4 cut(s) 494, 526, 770, 802
FaqI GGGAC 1 cut(s) 1260
FauNDI CATATG 1 cut(s) 1180
Fnu4HI GCNGC 5 cut(s) 431, 627, 869, 1398, 1467
FokI GGATG 4 cut(s) 901, 1004, 1073, 1436
FriOI GRGCYC 2 cut(s) 822, 1029
Fsp4HI GCNGC 5 cut(s) 431, 627, 869, 1398, 1467
FspBI CTAG 6 cut(s) 255, 674, 698, 834, 851, 1473
GlaI GCGC 2 cut(s) 305, 1616
GluI GCNGC 5 cut(s) 431, 627, 869, 1398, 1467
HaeII RGCGCY 1 cut(s) 307
HaeIII GGCC 1 cut(s) 1229
HapII CCGG 2 cut(s) 427, 1085
HgaI GACGC 1 cut(s) 1013
HhaI GCGC 2 cut(s) 306, 1617
Hin6I GCGC 2 cut(s) 304, 1615
HinP1I GCGC 2 cut(s) 304, 1615
HincII GTYRAC 2 cut(s) 206, 1480
HindII GTYRAC 2 cut(s) 206, 1480
HindIII AAGCTT 3 cut(s) 508, 538, 744
HinfI GANTC 5 cut(s) 71, 173, 620, 758, 1668
HpaII CCGG 2 cut(s) 427, 1085
HphI GGTGA 4 cut(s) 125, 595, 605, 1699
Hpy166II GTNNAC 4 cut(s) 10, 206, 1374, 1480
Hpy188I TCNGA 6 cut(s) 44, 341, 561, 768, 917, 1536
Hpy188III TCNNGA 1 cut(s) 698
Hpy8I GTNNAC 4 cut(s) 10, 206, 1374, 1480
Hpy99I CGWCG 3 cut(s) 720, 917, 1007
HpyAV CCTTC 3 cut(s) 1147, 1151, 1552
HpyCH4III ACNGT 3 cut(s) 412, 1288, 1522
HpyCH4IV ACGT 4 cut(s) 355, 368, 718, 1675
HpyF10VI GCNNNNNNNGC 2 cut(s) 1148, 1190
HpyF3I CTNAG 4 cut(s) 434, 536, 560, 1580
HpySE526I ACGT 4 cut(s) 355, 368, 718, 1675
HspAI GCGC 2 cut(s) 304, 1615
LguI GCTCTTC 1 cut(s) 1017
LmnI GCTCC 2 cut(s) 817, 825
Lsp1109I GCAGC 5 cut(s) 417, 638, 855, 1384, 1453
LweI GCATC 1 cut(s) 283
MaeI CTAG 6 cut(s) 255, 674, 698, 834, 851, 1473
MaeII ACGT 4 cut(s) 355, 368, 718, 1675
MaeIII GTNAC 6 cut(s) 494, 719, 1298, 1345, 1636, 1687
MboII GAAGA 8 cut(s) 13, 587, 752, 755, 1034, 1173, 1637, 1673
MfeI CAATTG 1 cut(s) 1066
MhlI GDGCHC 3 cut(s) 822, 850, 1029
MlyI GAGTC 1 cut(s) 629
MmeI TCCRAC 2 cut(s) 67, 895
MnlI CCTC 9 cut(s) 231, 555, 693, 1132, 1219, 1237, 1258, 1451, 1564
Mph1103I ATGCAT 1 cut(s) 363
MroXI GAANNNNTTC 1 cut(s) 762
MseI TTAA 4 cut(s) 62, 1118, 1221, 1628
MslI CAYNNNNRTG 2 cut(s) 1363, 1676
MspI CCGG 2 cut(s) 427, 1085
MspR9I CCNGG 2 cut(s) 68, 1085
MunI CAATTG 1 cut(s) 1066
Mva1269I GAATGC 2 cut(s) 245, 361
MvaI CCWGG 1 cut(s) 68
MvnI CGCG 1 cut(s) 1617
MwoI GCNNNNNNNGC 2 cut(s) 1148, 1190
NciI CCSGG 1 cut(s) 1085
NcoI CCATGG 1 cut(s) 382
NdeI CATATG 1 cut(s) 1180
NmeAIII GCCGAG 1 cut(s) 94
NmuCI GTSAC 2 cut(s) 1345, 1687
NsiI ATGCAT 1 cut(s) 363
NspI RCATGY 2 cut(s) 526, 1466
OliI CACNNNNGTG 1 cut(s) 1676
PaeI GCATGC 1 cut(s) 1466
PciI ACATGT 1 cut(s) 522
PciSI GCTCTTC 1 cut(s) 1017
PctI GAATGC 2 cut(s) 245, 361
PdmI GAANNNNTTC 1 cut(s) 762
PfeI GAWTC 4 cut(s) 71, 173, 758, 1668
PfoI TCCNGGA 2 cut(s) 66, 1083
PkrI GCNGC 5 cut(s) 432, 628, 870, 1399, 1468
PleI GAGTC 1 cut(s) 628
PmaCI CACGTG 1 cut(s) 1676
PmlI CACGTG 1 cut(s) 1676
PpsI GAGTC 1 cut(s) 628
Ppu21I YACGTR 1 cut(s) 1676
PscI ACATGT 1 cut(s) 522
PshBI ATTAAT 1 cut(s) 62
Psp124BI GAGCTC 2 cut(s) 822, 1029
Psp6I CCWGG 1 cut(s) 66
PspCI CACGTG 1 cut(s) 1676
PspGI CCWGG 1 cut(s) 66
PspPI GGNCC 1 cut(s) 1228
RsaI GTAC 3 cut(s) 902, 938, 1015
RsaNI GTAC 3 cut(s) 901, 937, 1014
RseI CAYNNNNRTG 2 cut(s) 1363, 1676
SacI GAGCTC 2 cut(s) 822, 1029
SapI GCTCTTC 1 cut(s) 1017
SaqAI TTAA 4 cut(s) 62, 1118, 1221, 1628
SatI GCNGC 5 cut(s) 431, 627, 869, 1398, 1467
Sau96I GGNCC 1 cut(s) 1228
ScaI AGTACT 1 cut(s) 938
SchI GAGTC 1 cut(s) 629
ScrFI CCNGG 2 cut(s) 68, 1085
SduI GDGCHC 3 cut(s) 822, 850, 1029
SfaNI GCATC 1 cut(s) 283
SmiMI CAYNNNNRTG 2 cut(s) 1363, 1676
SmlI CTYRAG 3 cut(s) 192, 617, 666
SmoI CTYRAG 3 cut(s) 192, 617, 666
SpeI ACTAGT 1 cut(s) 254
SphI GCATGC 1 cut(s) 1466
SsiI CCGC 1 cut(s) 118
SspI AATATT 1 cut(s) 987
SspMI CTAG 6 cut(s) 255, 674, 698, 834, 851, 1473
SstI GAGCTC 2 cut(s) 822, 1029
StyD4I CCNGG 2 cut(s) 66, 1083
StyI CCWWGG 2 cut(s) 307, 382
TaaI ACNGT 3 cut(s) 412, 1288, 1522
TaiI ACGT 4 cut(s) 358, 371, 721, 1678
TaqI TCGA 3 cut(s) 728, 1210, 1719
TatI WGTACW 2 cut(s) 900, 936
TfiI GAWTC 4 cut(s) 71, 173, 758, 1668
Tru1I TTAA 4 cut(s) 62, 1118, 1221, 1628
Tru9I TTAA 4 cut(s) 62, 1118, 1221, 1628
TscAI CASTG 4 cut(s) 415, 1381, 1515, 1731
TseFI GTSAC 2 cut(s) 1345, 1687
TseI GCWGC 5 cut(s) 430, 626, 868, 1397, 1466
Tsp45I GTSAC 2 cut(s) 1345, 1687
TspRI CASTG 4 cut(s) 415, 1381, 1515, 1731
VspI ATTAAT 1 cut(s) 62
XapI RAATTY 2 cut(s) 36, 127
XbaI TCTAGA 1 cut(s) 697
XceI RCATGY 2 cut(s) 526, 1466
XcmI CCANNNNNNNNNTGG 1 cut(s) 941
XmnI GAANNNNTTC 1 cut(s) 762
XspI CTAG 6 cut(s) 255, 674, 698, 834, 851, 1473
ZrmI AGTACT 1 cut(s) 938
Zsp2I ATGCAT 1 cut(s) 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.