Rorug01G0060200

Ubiquitin-binding WIYLD domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000053
Physical Location & Seq
Forward (+)
9940636 .. 9944843
4208 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0060200.1

Sequence Viewer

Length: 3183 bp
ATGGGATTTTTGTCTGTGAAATTGCTTTTGATATCCTCAGTTTTTAATGCAATATTCATTTTCTGTTCTAGCTCCTCCATGCAACTAAACCATCCCTTAACCACGAGACTTGGGGGGAATGAGACGGATAGGCTGGCACTGCTAGCAATCAAAGCTCAAATAAAACACGATCCCAACCAGGTCACAAGCTCATGGAATGAATCCCTTCATTTTTGCCTGTGGCACGGCATCACCTGCAGTCGACGACATCGCCAAAGGGTGACGAAGCTGGAGCTGCTCTCTCTAGCTTTGGGGGGATCCATATCTCCACACATAGGAAATCTAAGCTTCTTAAGAGTGCTAAATCTCGAGAACAATAGCTTCACTCATCAAATCCCTCCTCAAATTGGGCATTTGCATAGATTGCAGGTATTGCGTCTAAAGGAAAACTCACTTACTGGCACCATTCCTGCCAATATATCAAATTGCTTCAAACTCAGCACTCTCCATCTTGGTCACAACAATCTAGTGGGTAAAATTCCTCCCAAACTGAGTTCTTTGTCAAAGCTTCAGTTATTTTCTTTGCAATTGAACAATTTAACAGGAGAGATCCCTCCTTCTTTGGGAAACCTTTCATCTATGGAGAAATTTGGTTTAGGTCCTAACTACTTAGAAGGAAGCATCCCTAGTTCTCTATGCCAGTTGAAAAAATTAACAGTATTCTTATTGTCCGTAAATAGGTTGTCTGGCATCATCCCTTCCTGCATTTATAACCTCTCTGGTATAGTTGAGTTTGACGTAATACAAAACCAAATTCATGGAAGTCTTCCATCAAATTTGAACCATGCTTTTACCAACCTTCAATTCTTTTCCATTAGTTTTAATCAATTTACTGGTACCATCCCTTCGTCAATATCCAATGCGACAAATCTTATGACGTTTCAATGTAGTTCTAACATACTCACTGGACATGTGCCAAATTTACGAAATCATCATAACCTTGTGACATTCAACGTCGCCAATAATAATCTTGGAAGCGGTAAACTAGGTGACTTGAGTTTTGTCTCAGACTTGATCAATGCCACACAGCTGACAACGTTGGATTACGGCTACAACAAATTTGGAGGAACATTTCCAACATCAATATTCAATCTCTCAACCAATCTCCAAAAGCTTGCAATTGGAGGAAACTACCTACATGGAAGCATCCCAACTGGGTTAGGGAATCTTGGGAACTTGCTAGTACTCGGTATGGAGAAAAACAACTTCAGAGGTAGCATCCCCGCTGACATTGGGAAAGTTTTAAGGCTTGGGTTAATTTCTTTTTATAGCAACAAATTATCAGGGAGCATTCCATCTTCTCTTGGAAATTTAACAATGTTAAACAGTCTTTACTTGGATAGAAATAATCTCAATGGCAGCATCCCTTCAAGCCTAGGAGAATGTCACGGGTTGCAAGAGTTGGATCTTTCTGAAAACAGCTTAGAAGGCAAAATACCTCAACAATTACTTAATGGTCTCCTCTCTTTATCAATTTCTTTGAACTTGTCAACAAACCACTTTACTGGTTCCCTTCCCGTTGAGATTGGAAAGTTGAAGGCTCTAGGTAAGCTAGACATTTCTAACAACATGTTATCTGGAGAACTCCCTAGTAGCCTCGGTAGTTGTCAAAGTTTAGAAGTCCTACACTTGCAAGGCAACTTCTTCAACGGGCTCATCCCTTCATCTATGAAGGAATTGAGAGGGATTCAAGATTTAGACCTTTCTCGCAACAATTTCTCTGGAGATATTCCACAGTTCTTCGAGGGCTTTCGAAACTTGGAGAATCTCAATCTATCCTTCAATCAATTTTGGGGAGCAGTACCAATTGATGGTGTTTTTAAGAATGCAAGTGCGACTTCAGTTGCTGGAAACACCAGACTCTGTGGCGGTATTGCTAACCTTCGACTTCCTGTGTGTAAGTCCCATGGAGGAGGATTGTCCCGTGAAATGAAACTAATGATCTCTTTGGTCTCTGGGTTCACACTTTTGGGATTGGCTATGGTGCTGTCTTTTTTCCTTCTTTGTAAGAAAATGAAAGAAACTAAATTAAGCACTTTGGAGAACTCTATTTTGCAAGTTTCATACGCTACACTCTTGAAAGCTACTGATGGTTTTTCTTCAACTAATTTGATTGGTGTGGGTGCTTTTGGGTCTGTGTACAAAGGAATTCTTGTTGATGATAGAGTTGTCGCTGTGAAGGTACTTAACATGTTACACCAAGGAGCATCTAAGAGTTTCATGGCTGAATGTAAGGCTTTGAGAAATATCAGGCATCGAAATCTGGCCAAGATCTTAACTGTATGTTCAAGTAATGATTTCAGCGGCAATGATTTTAGGGCTCTTGTTTACGAGTTCATGGACAACGGGAGCTTAGATGAGTGGTTGCATCCATCCACTAGAAATGAAGAGGTGATAGAAGCACCCAAAAGTTTAAGCCTTGTTCAGAGGCTCAACATTGCCATTGAGGTTGCTTGTGCATTGGATTATCTTCACAACCACTGTGAAACACCAATAGTTCATTGTGATCTCAAGCCAAGTAATGTTCTTTTGGACAATGACCTGACTGGACATGTTTCTGACTTTGGGCTTTCAAGGTTTCTCTCGGAACCAACCAATAATGTTTCTATAAATCAATCAAGCTCCATCGGAATCAAAGGATCTGTTGGTTATGCTGCTCCAGAGTATGGTATGGGAAGTGGAGTGTCGACATATGGGGATGTCTACAGCTTTGGCATTCTCTTGTTAGAAATGTTTACAGGGAAGAGACCCACTGATCCCATGTTCAGTGACGGCTTGAATCTTCATTATTTTGTGAAGACAGCTCTCCCTGAAGGTGTTGCAGACATTGCAGATTTGCTTGTTCTTCAAGGACGCAATATAAACGTCGAAGAAGCCCACACACAGAGTATGAGAGAACAAAAAATTGATGGGTGCTTGACTTTGATATTTGAAATTGGAATTGCCTGTTCTGCCGAATCCCCAACAAACCGAAAGGATATTAGTGATGTTGCATCTGAATTGCACGCAATTAGGGGTCAGAATCCTCTAAATGATTCCAGCCTTGGAGGCCAAGTCTACTTGATGCCTAAGCTTAGCCTCTGTAATCTCTTTGTAAATCTGTGTTTAATACTTTCCGTACTACATGTATCTGGTGTAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WIYLD

Protein Analysis

1060

Amino Acids

115.08

Weight (kDa)

7.59

Isoelectric Point (pI)

31.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 41 - 80 6.8e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 96 - 231 3.5e-11 Leucine-rich repeat region
LRR_14 PF23598 471 - 604 3.8e-10 Leucine-rich repeat region
LRR_8 PF13855 551 - 610 4.4e-09 Leucine rich repeat
Pkinase PF00069 714 - 932 1.7e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 716 - 1014 5.1e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 750
AarI CACCTGC 1 cut(s) 242
Acc36I ACCTGC 2 cut(s) 242, 397
Acc65I GGTACC 1 cut(s) 875
AccB1I GGYRCC 2 cut(s) 440, 875
AccB7I CCANNNNNTGG 2 cut(s) 1850, 2705
AccI GTMKAC 4 cut(s) 241, 2726, 2742, 3096
AciI CCGC 4 cut(s) 1017, 1263, 1908, 2343
AclI AACGTT 1 cut(s) 1076
AclWI GGATC 7 cut(s) 164, 291, 304, 583, 1452, 2686, 2789
AcoI YGGCCR 1 cut(s) 2304
AcsI RAATTY 8 cut(s) 516, 626, 792, 814, 958, 1097, 1348, 2187
AcuI CTGAAG 4 cut(s) 533, 1231, 1863, 2871
AfaI GTAC 6 cut(s) 877, 1224, 1842, 2180, 2223, 3159
AfiI CCNNNNNNNGG 6 cut(s) 314, 386, 602, 717, 1850, 2705
AflII CTTAAG 1 cut(s) 331
AflIII ACRYGT 5 cut(s) 949, 1608, 2229, 2590, 3163
AjnI CCWGG 1 cut(s) 177
AjuI GAANNNNNNNTTGG 2 cut(s) 827, 859
Alw26I GTCTC 6 cut(s) 100, 116, 1048, 1502, 1996, 2779
AlwI GGATC 7 cut(s) 164, 291, 304, 583, 1452, 2686, 2789
AlwNI CAGNNNCTG 2 cut(s) 1886, 1902
Ama87I CYCGRG 1 cut(s) 347
AoxI GGCC 2 cut(s) 2304, 3088
ApeKI GCWGC 3 cut(s) 274, 1398, 2693
ApoI RAATTY 8 cut(s) 516, 626, 792, 814, 958, 1097, 1348, 2187
ArsI GACNNNNNNTTYG 2 cut(s) 1022, 1054
Asp700I GAANNNNTTC 2 cut(s) 204, 610
Asp718I GGTACC 1 cut(s) 875
AspA2I CCTAGG 1 cut(s) 1414
AspS9I GGNCC 1 cut(s) 638
AsuHPI GGTGA 4 cut(s) 223, 271, 1040, 2443
AsuII TTCGAA 1 cut(s) 1792
AsuNHI GCTAGC 1 cut(s) 142
AvaI CYCGRG 1 cut(s) 347
AvaII GGWCC 1 cut(s) 638
AvrII CCTAGG 1 cut(s) 1414
BalI TGGCCA 1 cut(s) 2306
BamHI GGATCC 1 cut(s) 296
BanI GGYRCC 2 cut(s) 440, 875
BanII GRGCYC 2 cut(s) 1695, 2362
BarI GAAGNNNNNNTAC 4 cut(s) 868, 900, 1458, 1490
BauI CACGAG 1 cut(s) 103
BbsI GAAGAC 2 cut(s) 797, 2843
BbvI GCAGC 3 cut(s) 261, 1410, 2680
BceAI ACGGC 3 cut(s) 241, 1102, 2827
BciT130I CCWGG 1 cut(s) 179
BclI TGATCA 1 cut(s) 1053
BcoDI GTCTC 6 cut(s) 100, 116, 1048, 1502, 1996, 2779
BfmI CTRYAG 2 cut(s) 235, 2743
BfrI CTTAAG 1 cut(s) 331
BfuAI ACCTGC 2 cut(s) 242, 397
BglI GCCNNNNNGGC 1 cut(s) 3087
BglII AGATCT 1 cut(s) 2310
BisI GCNGC 4 cut(s) 275, 1399, 2344, 2694
BlnI CCTAGG 1 cut(s) 1414
BlpI GCTNAGC 1 cut(s) 3113
BlsI GCNGC 4 cut(s) 276, 1400, 2345, 2695
BmcAI AGTACT 1 cut(s) 1224
Bme1390I CCNGG 1 cut(s) 179
Bme18I GGWCC 1 cut(s) 638
BmeT110I CYCGRG 1 cut(s) 347
BmgT120I GGNCC 1 cut(s) 638
BmiI GGNNCC 5 cut(s) 298, 442, 877, 1549, 2628
BmrFI CCNGG 1 cut(s) 179
BmrI ACTGGG 1 cut(s) 1203
BmtI GCTAGC 1 cut(s) 146
BmuI ACTGGG 1 cut(s) 1203
BpiI GAAGAC 2 cut(s) 797, 2843
BplI GAGNNNNNCTC 2 cut(s) 263, 295
BpmI CTGGAG 4 cut(s) 290, 1638, 1782, 2682
Bpu10I CCTNAGC 1 cut(s) 3108
Bpu1102I GCTNAGC 1 cut(s) 3113
Bpu14I TTCGAA 1 cut(s) 1792
BpuEI CTTGAG 2 cut(s) 1054, 2534
BsaBI GATNNNNATC 1 cut(s) 301
BsaI GGTCTC 3 cut(s) 1502, 1996, 2779
BsaJI CCNNGG 5 cut(s) 1414, 1636, 1945, 2239, 3082
BsaXI ACNNNNNCTCC 2 cut(s) 3078, 3108
Bsc4I CCNNNNNNNGG 6 cut(s) 314, 386, 602, 717, 1850, 2705
Bse1I ACTGG 7 cut(s) 442, 679, 877, 949, 1198, 1549, 2590
Bse3DI GCAATG 3 cut(s) 2353, 2475, 2865
Bse8I GATNNNNATC 1 cut(s) 301
BseBI CCWGG 1 cut(s) 179
BseDI CCNNGG 5 cut(s) 1414, 1636, 1945, 2239, 3082
BseJI GATNNNNATC 1 cut(s) 301
BseLI CCNNNNNNNGG 6 cut(s) 314, 386, 602, 717, 1850, 2705
BseMI GCAATG 3 cut(s) 2353, 2475, 2865
BseMII CTCAG 4 cut(s) 51, 490, 521, 1059
BseNI ACTGG 7 cut(s) 442, 679, 877, 949, 1198, 1549, 2590
BseRI GAGGAG 4 cut(s) 64, 369, 1490, 1965
BseXI GCAGC 3 cut(s) 261, 1410, 2680
BshFI GGCC 2 cut(s) 2306, 3090
BshNI GGYRCC 2 cut(s) 440, 875
BsiHKCI CYCGRG 1 cut(s) 347
BslFI GGGAC 2 cut(s) 1927, 1945
BslI CCNNNNNNNGG 6 cut(s) 314, 386, 602, 717, 1850, 2705
BsmAI GTCTC 6 cut(s) 100, 116, 1048, 1502, 1996, 2779
BsmBI CGTCTC 1 cut(s) 116
BsmFI GGGAC 2 cut(s) 1927, 1945
BsmI GAATGC 3 cut(s) 1329, 1870, 2754
BsnI GGCC 2 cut(s) 2306, 3090
Bso31I GGTCTC 3 cut(s) 1502, 1996, 2779
BsoBI CYCGRG 1 cut(s) 347
Bsp119I TTCGAA 1 cut(s) 1792
Bsp1286I GDGCHC 2 cut(s) 1695, 2362
Bsp1407I TGTACA 1 cut(s) 2178
Bsp1720I GCTNAGC 1 cut(s) 3113
Bsp19I CCATGG 1 cut(s) 1945
BspACI CCGC 4 cut(s) 1017, 1263, 1908, 2343
BspANI GGCC 2 cut(s) 2306, 3090
BspCNI CTCAG 4 cut(s) 50, 489, 522, 1058
BspLI GGNNCC 5 cut(s) 298, 442, 877, 1549, 2628
BspMAI CTGCAG 1 cut(s) 239
BspMI ACCTGC 2 cut(s) 242, 397
BspOI GCTAGC 1 cut(s) 146
BspPI GGATC 7 cut(s) 164, 291, 304, 583, 1452, 2686, 2789
BspT104I TTCGAA 1 cut(s) 1792
BspT107I GGYRCC 2 cut(s) 440, 875
BspTI CTTAAG 1 cut(s) 331
BspTNI GGTCTC 3 cut(s) 1502, 1996, 2779
BsrDI GCAATG 3 cut(s) 2353, 2475, 2865
BsrGI TGTACA 1 cut(s) 2178
BsrI ACTGG 7 cut(s) 442, 679, 877, 949, 1198, 1549, 2590
BssECI CCNNGG 5 cut(s) 1414, 1636, 1945, 2239, 3082
BssSI CACGAG 1 cut(s) 103
BssT1I CCWWGG 4 cut(s) 1414, 1945, 2239, 3082
Bst2BI CACGAG 1 cut(s) 103
Bst2UI CCWGG 1 cut(s) 179
Bst4CI ACNGT 5 cut(s) 697, 1367, 1776, 2320, 2522
Bst6I CTCTTC 2 cut(s) 2421, 2777
BstAFI CTTAAG 1 cut(s) 331
BstAPI GCANNNNNTGC 4 cut(s) 234, 403, 412, 2867
BstAUI TGTACA 1 cut(s) 2178
BstBI TTCGAA 1 cut(s) 1792
BstC8I GCNNGC 4 cut(s) 135, 144, 1155, 3045
BstDSI CCRYGG 1 cut(s) 1945
BstMAI GTCTC 6 cut(s) 100, 116, 1048, 1502, 1996, 2779
BstNI CCWGG 1 cut(s) 179
BstNSI RCATGY 5 cut(s) 953, 1612, 2233, 2594, 3167
BstSCI CCNGG 1 cut(s) 177
BstSFI CTRYAG 2 cut(s) 235, 2743
BstV1I GCAGC 3 cut(s) 261, 1410, 2680
BstV2I GAAGAC 2 cut(s) 797, 2843
BstX2I RGATCY 5 cut(s) 296, 588, 1444, 2310, 2678
BstXI CCANNNNNNTGG 2 cut(s) 797, 1544
BstYI RGATCY 5 cut(s) 296, 588, 1444, 2310, 2678
BsuRI GGCC 2 cut(s) 2306, 3090
BtgI CCRYGG 1 cut(s) 1945
BtgZI GCGATG 1 cut(s) 233
BtsI GCAGTG 1 cut(s) 137
BtsIMutI CAGTG 5 cut(s) 137, 942, 2518, 2790, 2812
BveI ACCTGC 2 cut(s) 242, 397
Cac8I GCNNGC 4 cut(s) 135, 144, 1155, 3045
CaiI CAGNNNCTG 2 cut(s) 1886, 1902
Cfr13I GGNCC 1 cut(s) 638
CseI GACGC 2 cut(s) 404, 2901
CsiI ACCWGGT 1 cut(s) 177
Csp6I GTAC 6 cut(s) 876, 1223, 1841, 2179, 2222, 3158
CspCI CAANNNNNGTGG 2 cut(s) 91, 126
CviQI GTAC 6 cut(s) 876, 1223, 1841, 2179, 2222, 3158
EaeI YGGCCR 1 cut(s) 2304
Eam1104I CTCTTC 2 cut(s) 2421, 2777
EarI CTCTTC 2 cut(s) 2421, 2777
Eco130I CCWWGG 4 cut(s) 1414, 1945, 2239, 3082
Eco24I GRGCYC 2 cut(s) 1695, 2362
Eco31I GGTCTC 3 cut(s) 1502, 1996, 2779
Eco32I GATATC 1 cut(s) 33
Eco47I GGWCC 1 cut(s) 638
Eco57I CTGAAG 4 cut(s) 533, 1231, 1863, 2871
Eco88I CYCGRG 1 cut(s) 347
EcoO109I RGGNCCY 1 cut(s) 638
EcoRI GAATTC 1 cut(s) 2187
EcoRII CCWGG 1 cut(s) 177
EcoRV GATATC 1 cut(s) 33
EcoT14I CCWWGG 4 cut(s) 1414, 1945, 2239, 3082
EcoT38I GRGCYC 2 cut(s) 1695, 2362
ErhI CCWWGG 4 cut(s) 1414, 1945, 2239, 3082
Esp3I CGTCTC 1 cut(s) 116
FalI AAGNNNNNCTT 4 cut(s) 1861, 1893, 2175, 2207
FaqI GGGAC 2 cut(s) 1927, 1945
FauI CCCGC 1 cut(s) 1270
FauNDI CATATG 1 cut(s) 2731
FbaI TGATCA 1 cut(s) 1053
FblI GTMKAC 4 cut(s) 241, 2726, 2742, 3096
Fnu4HI GCNGC 4 cut(s) 275, 1399, 2344, 2694
FriOI GRGCYC 2 cut(s) 1695, 2362
Fsp4HI GCNGC 4 cut(s) 275, 1399, 2344, 2694
GluI GCNGC 4 cut(s) 275, 1399, 2344, 2694
GsuI CTGGAG 4 cut(s) 290, 1638, 1782, 2682
HaeIII GGCC 2 cut(s) 2306, 3090
HgaI GACGC 2 cut(s) 404, 2901
HincII GTYRAC 3 cut(s) 242, 1530, 2727
HindII GTYRAC 3 cut(s) 242, 1530, 2727
HindIII AAGCTT 4 cut(s) 325, 545, 1151, 3110
HphI GGTGA 4 cut(s) 223, 271, 1040, 2443
Hpy188I TCNGA 9 cut(s) 1048, 1250, 1453, 2466, 2599, 2626, 2669, 3037, 3060
Hpy188III TCNNGA 7 cut(s) 347, 349, 1617, 1730, 1761, 2116, 2699
Hpy99I CGWCG 3 cut(s) 246, 998, 2909
HpyCH4III ACNGT 5 cut(s) 697, 1367, 1776, 2320, 2522
HpyCH4IV ACGT 5 cut(s) 777, 917, 993, 1076, 2904
HpySE526I ACGT 5 cut(s) 777, 917, 993, 1076, 2904
KpnI GGTACC 1 cut(s) 879
Ksp22I TGATCA 1 cut(s) 1053
LmnI GCTCC 8 cut(s) 77, 271, 1326, 1835, 2243, 2388, 2666, 2701
Lsp1109I GCAGC 3 cut(s) 261, 1410, 2680
MabI ACCWGGT 1 cut(s) 177
MaeII ACGT 5 cut(s) 777, 917, 993, 1076, 2904
MaeIII GTNAC 8 cut(s) 181, 259, 494, 982, 1028, 1424, 2232, 2807
MfeI CAATTG 3 cut(s) 566, 1158, 1845
MflI RGATCY 5 cut(s) 296, 588, 1444, 2310, 2678
MhlI GDGCHC 2 cut(s) 1695, 2362
MlsI TGGCCA 1 cut(s) 2306
MluNI TGGCCA 1 cut(s) 2306
MlyI GAGTC 1 cut(s) 1893
MmeI TCCRAC 3 cut(s) 1059, 1139, 1422
Mox20I TGGCCA 1 cut(s) 2306
MroXI GAANNNNTTC 2 cut(s) 204, 610
MscI TGGCCA 1 cut(s) 2306
Msp20I TGGCCA 1 cut(s) 2306
MspA1I CMGCKG 3 cut(s) 1069, 1265, 2343
MspCI CTTAAG 1 cut(s) 331
MspR9I CCNGG 1 cut(s) 179
MunI CAATTG 3 cut(s) 566, 1158, 1845
Mva1269I GAATGC 3 cut(s) 1329, 1870, 2754
MvaI CCWGG 1 cut(s) 179
NcoI CCATGG 1 cut(s) 1945
NdeI CATATG 1 cut(s) 2731
NheI GCTAGC 1 cut(s) 142
NlaIV GGNNCC 5 cut(s) 298, 442, 877, 1549, 2628
NmuCI GTSAC 7 cut(s) 181, 259, 494, 982, 1028, 1424, 2807
NspI RCATGY 5 cut(s) 953, 1612, 2233, 2594, 3167
NspV TTCGAA 1 cut(s) 1792
PaeR7I CTCGAG 1 cut(s) 347
PaqCI CACCTGC 1 cut(s) 242
PciI ACATGT 5 cut(s) 949, 1608, 2229, 2590, 3163
PctI GAATGC 3 cut(s) 1329, 1870, 2754
PdmI GAANNNNTTC 2 cut(s) 204, 610
PfeI GAWTC 9 cut(s) 200, 1204, 1726, 1804, 2670, 2818, 2996, 3061, 3074
PflMI CCANNNNNTGG 2 cut(s) 1850, 2705
PkrI GCNGC 4 cut(s) 276, 1400, 2345, 2695
PleI GAGTC 1 cut(s) 1893
PpsI GAGTC 1 cut(s) 1893
PpuMI RGGWCCY 1 cut(s) 638
PscI ACATGT 5 cut(s) 949, 1608, 2229, 2590, 3163
PsiI TTATAA 1 cut(s) 750
Psp1406I AACGTT 1 cut(s) 1076
Psp5II RGGWCCY 1 cut(s) 638
Psp6I CCWGG 1 cut(s) 177
PspGI CCWGG 1 cut(s) 177
PspN4I GGNNCC 5 cut(s) 298, 442, 877, 1549, 2628
PspPI GGNCC 1 cut(s) 638
PspPPI RGGWCCY 1 cut(s) 638
PstI CTGCAG 1 cut(s) 239
PstNI CAGNNNCTG 2 cut(s) 1886, 1902
PsuI RGATCY 5 cut(s) 296, 588, 1444, 2310, 2678
PvuII CAGCTG 1 cut(s) 1069
RsaI GTAC 6 cut(s) 877, 1224, 1842, 2180, 2223, 3159
RsaNI GTAC 6 cut(s) 876, 1223, 1841, 2179, 2222, 3158
SalI GTCGAC 2 cut(s) 240, 2725
SatI GCNGC 4 cut(s) 275, 1399, 2344, 2694
Sau96I GGNCC 1 cut(s) 638
ScaI AGTACT 1 cut(s) 1224
SchI GAGTC 1 cut(s) 1893
ScrFI CCNGG 1 cut(s) 179
SduI GDGCHC 2 cut(s) 1695, 2362
SexAI ACCWGGT 1 cut(s) 177
SfcI CTRYAG 2 cut(s) 235, 2743
Sfr274I CTCGAG 1 cut(s) 347
SfuI TTCGAA 1 cut(s) 1792
SinI GGWCC 1 cut(s) 638
SlaI CTCGAG 1 cut(s) 347
SmlI CTYRAG 4 cut(s) 331, 347, 1033, 2549
SmoI CTYRAG 4 cut(s) 331, 347, 1033, 2549
SsiI CCGC 4 cut(s) 1017, 1263, 1908, 2343
SspI AATATT 2 cut(s) 54, 1125
StyD4I CCNGG 1 cut(s) 177
StyI CCWWGG 4 cut(s) 1414, 1945, 2239, 3082
TaaI ACNGT 5 cut(s) 697, 1367, 1776, 2320, 2522
TaiI ACGT 5 cut(s) 780, 920, 996, 1079, 2907
TaqI TCGA 8 cut(s) 241, 348, 1782, 1792, 1924, 2296, 2726, 2907
TatI WGTACW 2 cut(s) 1222, 2178
TauI GCSGC 1 cut(s) 2346
TfiI GAWTC 9 cut(s) 200, 1204, 1726, 1804, 2670, 2818, 2996, 3061, 3074
TscAI CASTG 5 cut(s) 144, 949, 2525, 2797, 2812
TseFI GTSAC 7 cut(s) 181, 259, 494, 982, 1028, 1424, 2807
TseI GCWGC 3 cut(s) 274, 1398, 2693
Tsp45I GTSAC 7 cut(s) 181, 259, 494, 982, 1028, 1424, 2807
TspGWI ACGGA 3 cut(s) 140, 700, 3145
TspRI CASTG 5 cut(s) 144, 949, 2525, 2797, 2812
Van91I CCANNNNNTGG 2 cut(s) 1850, 2705
Vha464I CTTAAG 1 cut(s) 331
VpaK11BI GGWCC 1 cut(s) 638
XapI RAATTY 8 cut(s) 516, 626, 792, 814, 958, 1097, 1348, 2187
XceI RCATGY 5 cut(s) 953, 1612, 2233, 2594, 3167
XhoI CTCGAG 1 cut(s) 347
XmaJI CCTAGG 1 cut(s) 1414
XmiI GTMKAC 4 cut(s) 241, 2726, 2742, 3096
XmnI GAANNNNTTC 2 cut(s) 204, 610
ZrmI AGTACT 1 cut(s) 1224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.