Rroxscaffold_1G00043910

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0013641
Physical Location & Seq
Forward (+)
62657973 .. 62661234
3262 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00043910.1

Sequence Viewer

Length: 1725 bp
ATGGACTCGCGCCAGCATTTTGGTTTGAGTGAAACTGGTGCAGGCTATTTTGTAGGTTCTCATCCCACTGTTCCTAACAGGTTGCTTGGGTCTTTGAAATTTGATATTGGTAACTCACCAAATTCACCCTTTTCAACTCACTTTGATTCTGATACTGTTACTACGGTGAGTGACTGCCAGGAGCAACTTAGCTCCACAGAAAATCTCTCAGGAGTCAGCCATTCCTCTAACTCTCCATTTGAAACCAACAGTTATATTTCTCGGTTAAGCTTAAGCCCGTCTGTGGATTGTCGTCGAGACAGTCTGCAGCTCTCTTCTGGTAGGGCATCTTTGTTACAGGATGCAAATTGTAGCCCCAATATAAAATACGCTTTGCAGGAATTGGAGAGTGCTTTAATGGGGCCTGATGAAGAAGAAAAGGTCACCGCGACAAACATTTTTGGGGAAACTAGCAGGCGGAAGGGACAGAGATCTCTGTCATGGAACCAGGAGCGCGCAGGTACACATGTGATGCAGCGCCAGACTTCCTTTGTTTCAGGGCATAGACAGTCAGAGAAACGTCACAAGGTGCTTGATGAAGCATCTCCACAGGGTTTTCCGGCTGATAACCTGCGACAATTGCTGATTGCATGTGCTGAAGCACTTTCTGACAACAATATGGATGGTTTTAAGAAGTTGATTGAAAAGGCTAGAGGTGCTGTGTCTATCAGTGGAGAACCAATTCAGCGTCTTGGAGCTTACATGGTAGAAGGGCTGGTTGCAAGGAAGGAGGCTTCGGGCTCCAATATCTACCGTGCCCTCAGGTGCATGGAGCCTGAAAGCGATGACTTGCTCTCATACATGCACATTCTGTATGAGATCTGCCCCTATTTAAAGTTCGGTTACATGGCAGCCAATGGGGCCATTGCTGACGCCTGCAGAAATGAGGACCGTATCCACATTATAGACTTCCAGATTGCTCAGGGTACTCAATGGGTGACTCTCCTTCAAGCTCTTGCAGCAAGACCCAGTGGGGCTCCCCATGTACGAATTACAGGTATCGATGACCCTGTTTCTAAATATGCTCGTGGTGATGGCTTGGAGGCAGTAGGAAGACGGTTGAAAGCAATCTCTGAGAAATTTAACATCCCAGTTGAATTTCACGGGGTTCCAGTTTTTGCACCTGATGTCACACCGGACATGCTTGATGTCAGGCCTGGTGAGGCTCTAGCAGTGAACTTTCCCCTGCAGCTTCACCACACACCAGATGAAAGCGTTGATGTGAACAATCCAAGGGATGGGCTTCTGAGGATAGTCAAGTCACTATCTCCAAAGGTGACTACTTTGGTTGAGCAAGAATCAAACACGAATACGACCCCTTTCTTGAATCGGTTTATAGAGACTCTAGACTACTATTTGGCAATGTTTGAGTCTATTGACGTGACCCTGCCGAGGAACAGCAAGGAGAGGATCAATGTGGAGCAGCATTGTCTGGCTCGAGATATTGTAAATGTGATTGCCTGCGAAGGAAAGGAAAGGGTGGAACGCCACGAGCTCTTTGGAAAGTGGAAGTCCAGGTTGACAATGGCAGGATTTCGGCAATACCCATTAAGCTCATATGTCAACTCTGTCATAAGGAGTCTACTGAAGTGTTACTCAGAGCATTATAAGCTGGAGGAGAAGGATGGTGCTATGCTGTTGGGATGGAAGGAGAGGAACTTGGTATCAGCTTCTGCGTGGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

574

Amino Acids

63.96

Weight (kDa)

6.05

Isoelectric Point (pI)

52.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 204 - 573 3.5e-135 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1647
Acc36I ACCTGC 2 cut(s) 488, 618
AccB7I CCANNNNNTGG 1 cut(s) 1277
AccI GTMKAC 1 cut(s) 1621
AccII CGCG 3 cut(s) 10, 428, 495
AciI CCGC 2 cut(s) 426, 457
AclWI GGATC 1 cut(s) 1457
AcsI RAATTY 4 cut(s) 98, 121, 1118, 1136
AcuI CTGAAG 2 cut(s) 657, 1646
AcyI GRCGYC 1 cut(s) 912
AdeI CACNNNGTG 1 cut(s) 568
AfaI GTAC 3 cut(s) 502, 967, 1026
AfiI CCNNNNNNNGG 3 cut(s) 283, 1277, 1431
AflII CTTAAG 1 cut(s) 271
AflIII ACRYGT 1 cut(s) 505
AgsI TTSAA 8 cut(s) 97, 135, 242, 683, 989, 1102, 1136, 1366
AjiI CACGTC 1 cut(s) 1420
AjnI CCWGG 4 cut(s) 177, 486, 1195, 1553
Alw21I GWGCWC 1 cut(s) 1536
Alw26I GTCTC 2 cut(s) 291, 1373
AlwI GGATC 1 cut(s) 1457
AlwNI CAGNNNCTG 1 cut(s) 1712
Ama87I CYCGRG 1 cut(s) 1476
AoxI GGCC 3 cut(s) 401, 900, 1193
ApeKI GCWGC 6 cut(s) 307, 514, 890, 998, 1228, 1462
ApoI RAATTY 4 cut(s) 98, 121, 1118, 1136
Asp700I GAANNNNTTC 1 cut(s) 720
AspLEI GCGC 4 cut(s) 12, 495, 497, 519
AspS9I GGNCC 3 cut(s) 401, 900, 928
AsuHPI GGTGA 9 cut(s) 108, 117, 178, 415, 988, 1082, 1211, 1226, 1327
AvaI CYCGRG 1 cut(s) 1476
AvaII GGWCC 1 cut(s) 928
AxyI CCTNAGG 1 cut(s) 800
BaeGI GKGCMC 1 cut(s) 799
BaeI ACNNNNGTAYC 2 cut(s) 492, 525
BanII GRGCYC 3 cut(s) 782, 1018, 1536
BauI CACGAG 2 cut(s) 1065, 1529
BbsI GAAGAC 1 cut(s) 1099
Bbv12I GWGCWC 1 cut(s) 1536
BbvI GCAGC 6 cut(s) 319, 526, 902, 1010, 1240, 1474
BccI CCATC 5 cut(s) 656, 1067, 1271, 1658, 1677
BciT130I CCWGG 4 cut(s) 179, 488, 1197, 1555
BciVI GTATCC 1 cut(s) 944
BcoDI GTCTC 2 cut(s) 291, 1373
BfaI CTAG 4 cut(s) 450, 690, 1208, 1385
BfmI CTRYAG 3 cut(s) 305, 916, 1226
BfoI RGCGCY 1 cut(s) 520
BfrI CTTAAG 1 cut(s) 271
BfuAI ACCTGC 2 cut(s) 488, 618
BfuI GTATCC 1 cut(s) 944
BglI GCCNNNNNGGC 1 cut(s) 899
BglII AGATCT 2 cut(s) 470, 858
BisI GCNGC 6 cut(s) 308, 515, 891, 999, 1229, 1463
BlsI GCNGC 6 cut(s) 309, 516, 892, 1000, 1230, 1464
Bme1390I CCNGG 4 cut(s) 179, 488, 1197, 1555
Bme18I GGWCC 1 cut(s) 928
BmeT110I CYCGRG 1 cut(s) 1476
BmgBI CACGTC 1 cut(s) 1420
BmgT120I GGNCC 3 cut(s) 401, 900, 928
BmiI GGNNCC 7 cut(s) 402, 485, 781, 813, 901, 1017, 1149
BmrFI CCNGG 4 cut(s) 179, 488, 1197, 1555
BmrI ACTGGG 2 cut(s) 1002, 1124
BmsI GCATC 4 cut(s) 331, 335, 501, 590
BmuI ACTGGG 2 cut(s) 1002, 1124
BpiI GAAGAC 1 cut(s) 1099
BpmI CTGGAG 1 cut(s) 1673
Bpu10I CCTNAGC 1 cut(s) 960
Bsa29I ATCGAT 1 cut(s) 1041
BsaHI GRCGYC 1 cut(s) 912
BsaJI CCNNGG 2 cut(s) 1271, 1430
BsaWI WCCGGW 1 cut(s) 1174
Bsc4I CCNNNNNNNGG 3 cut(s) 283, 1277, 1431
Bse1I ACTGG 4 cut(s) 40, 1008, 1130, 1151
Bse21I CCTNAGG 1 cut(s) 800
Bse3DI GCAATG 2 cut(s) 903, 1407
BseBI CCWGG 4 cut(s) 179, 488, 1197, 1555
BseCI ATCGAT 1 cut(s) 1041
BseDI CCNNGG 2 cut(s) 1271, 1430
BseGI GGATG 7 cut(s) 61, 346, 667, 1125, 1282, 1669, 1688
BseLI CCNNNNNNNGG 3 cut(s) 283, 1277, 1431
BseMI GCAATG 2 cut(s) 903, 1407
BseMII CTCAG 6 cut(s) 222, 814, 974, 1104, 1277, 1650
BseNI ACTGG 4 cut(s) 40, 1008, 1130, 1151
BsePI GCGCGC 1 cut(s) 493
BseRI GAGGAG 1 cut(s) 1670
BseSI GKGCMC 1 cut(s) 799
BseXI GCAGC 6 cut(s) 319, 526, 902, 1010, 1240, 1474
BsgI GTGCAG 1 cut(s) 60
Bsh1236I CGCG 3 cut(s) 10, 428, 495
BshFI GGCC 3 cut(s) 403, 902, 1195
BshVI ATCGAT 1 cut(s) 1041
BsiHKAI GWGCWC 1 cut(s) 1536
BsiHKCI CYCGRG 1 cut(s) 1476
BsiSI CCGG 2 cut(s) 599, 1175
BslFI GGGAC 1 cut(s) 477
BslI CCNNNNNNNGG 3 cut(s) 283, 1277, 1431
BsmAI GTCTC 2 cut(s) 291, 1373
BsmFI GGGAC 1 cut(s) 477
BsnI GGCC 3 cut(s) 403, 902, 1195
BsoBI CYCGRG 1 cut(s) 1476
Bsp1286I GDGCHC 4 cut(s) 782, 799, 1018, 1536
Bsp143I GATC 3 cut(s) 470, 858, 1449
BspACI CCGC 2 cut(s) 426, 457
BspANI GGCC 3 cut(s) 403, 902, 1195
BspCNI CTCAG 6 cut(s) 221, 813, 973, 1105, 1278, 1649
BspDI ATCGAT 1 cut(s) 1041
BspFNI CGCG 3 cut(s) 10, 428, 495
BspLI GGNNCC 7 cut(s) 402, 485, 781, 813, 901, 1017, 1149
BspMAI CTGCAG 3 cut(s) 309, 920, 1230
BspMI ACCTGC 2 cut(s) 488, 618
BspPI GGATC 1 cut(s) 1457
BspTI CTTAAG 1 cut(s) 271
BsrDI GCAATG 2 cut(s) 903, 1407
BsrI ACTGG 4 cut(s) 40, 1008, 1130, 1151
BssECI CCNNGG 2 cut(s) 1271, 1430
BssHII GCGCGC 1 cut(s) 493
BssMI GATC 3 cut(s) 470, 858, 1449
BssNI GRCGYC 1 cut(s) 912
BssSI CACGAG 2 cut(s) 1065, 1529
BssT1I CCWWGG 1 cut(s) 1271
Bst2BI CACGAG 2 cut(s) 1065, 1529
Bst2UI CCWGG 4 cut(s) 179, 488, 1197, 1555
Bst4CI ACNGT 9 cut(s) 70, 157, 166, 251, 302, 549, 794, 932, 1098
Bst6I CTCTTC 1 cut(s) 319
BstACI GRCGYC 1 cut(s) 912
BstAFI CTTAAG 1 cut(s) 271
BstC8I GCNNGC 6 cut(s) 14, 43, 455, 495, 916, 1501
BstDEI CTNAG 7 cut(s) 188, 208, 800, 960, 1113, 1286, 1636
BstEII GGTNACC 1 cut(s) 421
BstF5I GGATG 7 cut(s) 61, 346, 667, 1125, 1282, 1669, 1688
BstFNI CGCG 3 cut(s) 10, 428, 495
BstH2I RGCGCY 1 cut(s) 520
BstHHI GCGC 4 cut(s) 12, 495, 497, 519
BstKTI GATC 3 cut(s) 473, 861, 1452
BstMAI GTCTC 2 cut(s) 291, 1373
BstMBI GATC 3 cut(s) 470, 858, 1449
BstMWI GCNNNNNNNGC 5 cut(s) 619, 695, 899, 998, 1648
BstNI CCWGG 4 cut(s) 179, 488, 1197, 1555
BstNSI RCATGY 4 cut(s) 509, 633, 844, 1183
BstPI GGTNACC 1 cut(s) 421
BstSCI CCNGG 4 cut(s) 177, 486, 1195, 1553
BstSFI CTRYAG 3 cut(s) 305, 916, 1226
BstSLI GKGCMC 1 cut(s) 799
BstUI CGCG 3 cut(s) 10, 428, 495
BstV1I GCAGC 6 cut(s) 319, 526, 902, 1010, 1240, 1474
BstV2I GAAGAC 1 cut(s) 1099
BstX2I RGATCY 2 cut(s) 470, 858
BstXI CCANNNNNNTGG 1 cut(s) 20
BstYI RGATCY 2 cut(s) 470, 858
Bsu15I ATCGAT 1 cut(s) 1041
Bsu36I CCTNAGG 1 cut(s) 800
BsuI GTATCC 1 cut(s) 944
BsuRI GGCC 3 cut(s) 403, 902, 1195
BsuTUI ATCGAT 1 cut(s) 1041
BtgZI GCGATG 1 cut(s) 837
BtrI CACGTC 1 cut(s) 1420
BtsCI GGATG 7 cut(s) 61, 346, 667, 1125, 1282, 1669, 1688
BtsI GCAGTG 1 cut(s) 1218
BtsIMutI CAGTG 5 cut(s) 66, 715, 1015, 1218, 1720
BveI ACCTGC 2 cut(s) 488, 618
Cac8I GCNNGC 6 cut(s) 14, 43, 455, 495, 916, 1501
CaiI CAGNNNCTG 1 cut(s) 1712
CfoI GCGC 4 cut(s) 12, 495, 497, 519
Cfr13I GGNCC 3 cut(s) 401, 900, 928
ClaI ATCGAT 1 cut(s) 1041
CseI GACGC 2 cut(s) 716, 920
Csp6I GTAC 3 cut(s) 501, 966, 1025
CviAII CATG 9 cut(s) 480, 506, 630, 742, 808, 841, 886, 1022, 1180
CviQI GTAC 3 cut(s) 501, 966, 1025
DdeI CTNAG 7 cut(s) 188, 208, 800, 960, 1113, 1286, 1636
DpnI GATC 3 cut(s) 472, 860, 1451
DpnII GATC 3 cut(s) 470, 858, 1449
DraI TTTAAA 1 cut(s) 873
DraIII CACNNNGTG 1 cut(s) 568
Eam1104I CTCTTC 1 cut(s) 319
EarI CTCTTC 1 cut(s) 319
EciI GGCGGA 1 cut(s) 472
Ecl136II GAGCTC 1 cut(s) 1534
Eco130I CCWWGG 1 cut(s) 1271
Eco147I AGGCCT 1 cut(s) 1195
Eco24I GRGCYC 3 cut(s) 782, 1018, 1536
Eco47I GGWCC 1 cut(s) 928
Eco53kI GAGCTC 1 cut(s) 1534
Eco57I CTGAAG 2 cut(s) 657, 1646
Eco81I CCTNAGG 1 cut(s) 800
Eco88I CYCGRG 1 cut(s) 1476
Eco91I GGTNACC 1 cut(s) 421
EcoICRI GAGCTC 1 cut(s) 1534
EcoO109I RGGNCCY 1 cut(s) 401
EcoO65I GGTNACC 1 cut(s) 421
EcoRII CCWGG 4 cut(s) 177, 486, 1195, 1553
EcoT14I CCWWGG 1 cut(s) 1271
EcoT38I GRGCYC 3 cut(s) 782, 1018, 1536
ErhI CCWWGG 1 cut(s) 1271
FaeI CATG 9 cut(s) 483, 509, 633, 745, 811, 844, 889, 1025, 1183
FaqI GGGAC 1 cut(s) 477
FatI CATG 9 cut(s) 479, 505, 629, 741, 807, 840, 885, 1021, 1179
FauNDI CATATG 1 cut(s) 1597
FblI GTMKAC 1 cut(s) 1621
Fnu4HI GCNGC 6 cut(s) 308, 515, 891, 999, 1229, 1463
FokI GGATG 7 cut(s) 48, 353, 674, 1112, 1289, 1676, 1695
FriOI GRGCYC 3 cut(s) 782, 1018, 1536
Fsp4HI GCNGC 6 cut(s) 308, 515, 891, 999, 1229, 1463
FspBI CTAG 4 cut(s) 450, 690, 1208, 1385
GlaI GCGC 4 cut(s) 11, 494, 496, 518
GluI GCNGC 6 cut(s) 308, 515, 891, 999, 1229, 1463
GsuI CTGGAG 1 cut(s) 1673
HaeII RGCGCY 1 cut(s) 520
HaeIII GGCC 3 cut(s) 403, 902, 1195
HapII CCGG 2 cut(s) 599, 1175
HgaI GACGC 2 cut(s) 716, 920
HhaI GCGC 4 cut(s) 12, 495, 497, 519
Hin1I GRCGYC 1 cut(s) 912
Hin1II CATG 9 cut(s) 483, 509, 633, 745, 811, 844, 889, 1025, 1183
Hin6I GCGC 4 cut(s) 10, 493, 495, 517
HinP1I GCGC 4 cut(s) 10, 493, 495, 517
HincII GTYRAC 2 cut(s) 1560, 1603
HindII GTYRAC 2 cut(s) 1560, 1603
HindIII AAGCTT 1 cut(s) 268
HinfI GANTC 9 cut(s) 5, 146, 213, 979, 1337, 1366, 1381, 1409, 1618
HpaII CCGG 2 cut(s) 599, 1175
HphI GGTGA 9 cut(s) 108, 117, 178, 415, 988, 1082, 1211, 1226, 1327
Hpy166II GTNNAC 6 cut(s) 503, 1216, 1264, 1560, 1603, 1622
Hpy188I TCNGA 6 cut(s) 151, 553, 649, 1114, 1287, 1639
Hpy188III TCNNGA 6 cut(s) 210, 296, 952, 1363, 1385, 1478
Hpy8I GTNNAC 6 cut(s) 503, 1216, 1264, 1560, 1603, 1622
Hpy99I CGWCG 1 cut(s) 297
HpyAV CCTTC 7 cut(s) 454, 743, 760, 995, 1499, 1654, 1681
HpyCH4III ACNGT 9 cut(s) 70, 157, 166, 251, 302, 549, 794, 932, 1098
HpyCH4IV ACGT 2 cut(s) 559, 1419
HpyF10VI GCNNNNNNNGC 5 cut(s) 619, 695, 899, 998, 1648
HpyF3I CTNAG 7 cut(s) 188, 208, 800, 960, 1113, 1286, 1636
HpySE526I ACGT 2 cut(s) 559, 1419
Hsp92I GRCGYC 1 cut(s) 912
Hsp92II CATG 9 cut(s) 483, 509, 633, 745, 811, 844, 889, 1025, 1183
HspAI GCGC 4 cut(s) 10, 493, 495, 517
Kzo9I GATC 3 cut(s) 470, 858, 1449
LmnI GCTCC 8 cut(s) 181, 197, 490, 734, 785, 811, 1021, 1459
Lsp1109I GCAGC 6 cut(s) 319, 526, 902, 1010, 1240, 1474
LweI GCATC 4 cut(s) 331, 335, 501, 590
MaeI CTAG 4 cut(s) 450, 690, 1208, 1385
MaeII ACGT 2 cut(s) 559, 1419
MalI GATC 3 cut(s) 472, 860, 1451
MboI GATC 3 cut(s) 470, 858, 1449
MboII GAAGA 4 cut(s) 306, 422, 425, 1104
MfeI CAATTG 1 cut(s) 617
MflI RGATCY 2 cut(s) 470, 858
MhlI GDGCHC 4 cut(s) 782, 799, 1018, 1536
MluCI AATT 9 cut(s) 98, 121, 346, 380, 617, 720, 1029, 1118, 1136
MlyI GAGTC 5 cut(s) 222, 973, 1375, 1418, 1627
MroXI GAANNNNTTC 1 cut(s) 720
MseI TTAA 7 cut(s) 266, 272, 395, 669, 872, 1122, 1589
MspCI CTTAAG 1 cut(s) 271
MspI CCGG 2 cut(s) 599, 1175
MspR9I CCNGG 4 cut(s) 179, 488, 1197, 1555
MunI CAATTG 1 cut(s) 617
MvaI CCWGG 4 cut(s) 179, 488, 1197, 1555
MvnI CGCG 3 cut(s) 10, 428, 495
MwoI GCNNNNNNNGC 5 cut(s) 619, 695, 899, 998, 1648
NdeI CATATG 1 cut(s) 1597
NdeII GATC 3 cut(s) 470, 858, 1449
NlaIII CATG 9 cut(s) 483, 509, 633, 745, 811, 844, 889, 1025, 1183
NlaIV GGNNCC 7 cut(s) 402, 485, 781, 813, 901, 1017, 1149
NmeAIII GCCGAG 1 cut(s) 1455
NmuCI GTSAC 8 cut(s) 170, 421, 560, 976, 1168, 1299, 1315, 1420
NspI RCATGY 4 cut(s) 509, 633, 844, 1183
PaeR7I CTCGAG 1 cut(s) 1476
PauI GCGCGC 1 cut(s) 493
PceI AGGCCT 1 cut(s) 1195
PciI ACATGT 1 cut(s) 505
PdmI GAANNNNTTC 1 cut(s) 720
PfeI GAWTC 3 cut(s) 146, 1337, 1366
PflMI CCANNNNNTGG 1 cut(s) 1277
PkrI GCNGC 6 cut(s) 309, 516, 892, 1000, 1230, 1464
PleI GAGTC 5 cut(s) 221, 973, 1375, 1417, 1626
PpsI GAGTC 5 cut(s) 221, 973, 1375, 1417, 1626
PscI ACATGT 1 cut(s) 505
PsiI TTATAA 1 cut(s) 1647
Psp124BI GAGCTC 1 cut(s) 1536
Psp6I CCWGG 4 cut(s) 177, 486, 1195, 1553
PspEI GGTNACC 1 cut(s) 421
PspGI CCWGG 4 cut(s) 177, 486, 1195, 1553
PspN4I GGNNCC 7 cut(s) 402, 485, 781, 813, 901, 1017, 1149
PspPI GGNCC 3 cut(s) 401, 900, 928
PstI CTGCAG 3 cut(s) 309, 920, 1230
PstNI CAGNNNCTG 1 cut(s) 1712
PsuI RGATCY 2 cut(s) 470, 858
PteI GCGCGC 1 cut(s) 493
RsaI GTAC 3 cut(s) 502, 967, 1026
RsaNI GTAC 3 cut(s) 501, 966, 1025
SacI GAGCTC 1 cut(s) 1536
SaqAI TTAA 7 cut(s) 266, 272, 395, 669, 872, 1122, 1589
SatI GCNGC 6 cut(s) 308, 515, 891, 999, 1229, 1463
Sau3AI GATC 3 cut(s) 470, 858, 1449
Sau96I GGNCC 3 cut(s) 401, 900, 928
SchI GAGTC 5 cut(s) 222, 973, 1375, 1418, 1627
ScrFI CCNGG 4 cut(s) 179, 488, 1197, 1555
SduI GDGCHC 4 cut(s) 782, 799, 1018, 1536
SfaNI GCATC 4 cut(s) 331, 335, 501, 590
SfcI CTRYAG 3 cut(s) 305, 916, 1226
Sfr274I CTCGAG 1 cut(s) 1476
SinI GGWCC 1 cut(s) 928
SlaI CTCGAG 1 cut(s) 1476
SmlI CTYRAG 2 cut(s) 271, 1476
SmoI CTYRAG 2 cut(s) 271, 1476
Sse9I AATT 9 cut(s) 98, 121, 346, 380, 617, 720, 1029, 1118, 1136
SseBI AGGCCT 1 cut(s) 1195
SsiI CCGC 2 cut(s) 426, 457
SspMI CTAG 4 cut(s) 450, 690, 1208, 1385
SstI GAGCTC 1 cut(s) 1536
StuI AGGCCT 1 cut(s) 1195
StyD4I CCNGG 4 cut(s) 177, 486, 1195, 1553
StyI CCWWGG 1 cut(s) 1271
TaaI ACNGT 9 cut(s) 70, 157, 166, 251, 302, 549, 794, 932, 1098
TaiI ACGT 2 cut(s) 562, 1422
TaqI TCGA 3 cut(s) 295, 1041, 1477
TasI AATT 9 cut(s) 98, 121, 346, 380, 617, 720, 1029, 1118, 1136
TfiI GAWTC 3 cut(s) 146, 1337, 1366
Tru1I TTAA 7 cut(s) 266, 272, 395, 669, 872, 1122, 1589
Tru9I TTAA 7 cut(s) 266, 272, 395, 669, 872, 1122, 1589
TscAI CASTG 4 cut(s) 73, 715, 1015, 1218
TseFI GTSAC 8 cut(s) 170, 421, 560, 976, 1168, 1299, 1315, 1420
TseI GCWGC 6 cut(s) 307, 514, 890, 998, 1228, 1462
Tsp45I GTSAC 8 cut(s) 170, 421, 560, 976, 1168, 1299, 1315, 1420
TspDTI ATGAA 3 cut(s) 423, 591, 1263
TspRI CASTG 4 cut(s) 73, 715, 1015, 1218
Van91I CCANNNNNTGG 1 cut(s) 1277
Vha464I CTTAAG 1 cut(s) 271
VpaK11BI GGWCC 1 cut(s) 928
XapI RAATTY 4 cut(s) 98, 121, 1118, 1136
XbaI TCTAGA 1 cut(s) 1384
XceI RCATGY 4 cut(s) 509, 633, 844, 1183
XcmI CCANNNNNNNNNTGG 2 cut(s) 1535, 1561
XhoI CTCGAG 1 cut(s) 1476
XmiI GTMKAC 1 cut(s) 1621
XmnI GAANNNNTTC 1 cut(s) 720
XspI CTAG 4 cut(s) 450, 690, 1208, 1385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.