Rh5BG253600

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0013641
Physical Location & Seq
Reverse (-)
31512781 .. 31514493
1713 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG253600.1

Sequence Viewer

Length: 1713 bp
ATGGACTCGCGCCAGCGTTTTGGTTTCAGTGAAACTGGTGCAGGCTATTTTGGAGGTTCTCATCCCACTGCTCCTAACAGGTTGCTTGGGTCTTTGAAATTAGATATTGGTAACTCACCAAATTCACACTTTTCAACTCACTTTGATTCTGATACTGTTACTACGCTGAGTGACTGCCAGGAGCAACTTAGCTCCACAGAAAATCTCTCAGGAGTCAGCCATTCCTCTAACTCTCCATTTGAAACCAACAGTTATATTTCTCGGTTAAGCTTAAGCCCTTCTGTGGATTGTCGTCGAGACAGTCTGCAGCTCTCTTCTGGTAGGGCATCTTTGTTACAGGATGCAAATTGTAGCCCCAATATAAAATACGCTTTGCAGGAATTGGAGAGTGCTTTAATGGGGCCTGATGAAGAAGAGGTCACCGCAACAAACATTTTTGGGGAAACTAGCAGGCGGAAGGGACAGAGATCTCTCCAGGAGCGCGCGGGTGCACATGTGATGCAGCGCCAGACTTCCTTTGTTTCAGGGCATAGACAGTCAGAGAAACGTCACAAGGTGCTTGATGAAGCATCTCCACAGGGTTTTCTGGCTGATAACCTGCGACAATTGCTGATTGCATGTGCTGAAGCACTTTCTGACAACAATATGGATGGTTTTGAGAAGTTGATTGAAAAGGCTAGAGGTGCTGTGTCTATCAGTGGAGAACCAATTCAGCGTCTTGGAGCTTACATGGTAGAAGGGCTGGTTGCAAGGAAGGAGGCTTCGGGCTCCAATTTCTACCGTGCCCTCAGGTGCAGGGAGCCTGAAAGCGATGACTTGCTCTCATACATGCACATTCTGTATGAGATCTGCCCCTATTTAAAGTTCGGTTACATGGCAGCCAATGGGGCCATTGCTGACGCCTGCAGAAATGAGGACCGTATCCACATTATAGACTTCCAGATTGCTCAAGGTACTCAATGGGTGACTCTCCTTCAAGCTCTTGCAGCAAGACCCAGTGGGGCTCCCCATGTACGAATTACAGGTATCGATGACCCTGTTTCTAAGTATGCTCGTGGTGATGGCTTGGAGGCAGTAGGAAGACGGTTGAAAGCAATCTCTGAGAAATTTAACATCCCAGTTGAATTTCACGGGGTTCCAGTTTTTGCACCTGATGTCACACCGGACATGCTTGATGTCAGGCCTGGTGAGGCTCTAGCAGTGAACTTTCCCCTGCAGCTTCACCACACACCAGATGAAAGCGTTGATGTGAACAATCCAAGGGATGGACTTCTGAGGATAGTCAAGTCACTATCTCCAAAGGTGACTACTTTGGTTGAGCAAGAATCAAACACGAATACAACCCCTTTCTTGAATCGGTTTATAGAGACTCTAGACTACTATTTGGCAATGTTTGAGTCTATTGACGTGACTCTGCCGAGGAACAGCAAGGAGAGGATCAACGTGGAGCAGCATTGTCTGGCTCGAGATATTGTAAATGTGATTGCCTGCGAAGGAAAGGAAAGGGTGGAACGCCACGAGCTCTTTGGGAAGTGGAAGTCCAGGTTGACAATGGCAGGATTTCGGCAATACCCATTAAGCTCATATGTCAACTCTGTCATAAGGAGTCTACTGAGGTGTTACTCAGAGCATTATAAGCTGGAGGAGAAGGATGGTGCTATGCTGTTGGGATGGAAGGAGAGGAACTTGGTATCAGCTTCTGCGTGGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

570

Amino Acids

63.52

Weight (kDa)

5.98

Isoelectric Point (pI)

51.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 200 - 569 3.6e-135 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1635
Acc36I ACCTGC 1 cut(s) 606
AccB7I CCANNNNNTGG 1 cut(s) 1265
AccI GTMKAC 1 cut(s) 1609
AccII CGCG 3 cut(s) 10, 483, 485
AciI CCGC 3 cut(s) 423, 454, 485
AclWI GGATC 1 cut(s) 1445
AcsI RAATTY 3 cut(s) 121, 1106, 1124
AcuI CTGAAG 1 cut(s) 645
AcyI GRCGYC 1 cut(s) 900
AdeI CACNNNGTG 1 cut(s) 556
AfaI GTAC 2 cut(s) 955, 1014
AfiI CCNNNNNNNGG 2 cut(s) 283, 1265
AflII CTTAAG 1 cut(s) 271
AflIII ACRYGT 1 cut(s) 493
AgsI TTSAA 8 cut(s) 97, 135, 242, 671, 977, 1090, 1124, 1354
AjiI CACGTC 1 cut(s) 1408
AjnI CCWGG 4 cut(s) 177, 474, 1183, 1541
Alw21I GWGCWC 2 cut(s) 493, 1524
Alw26I GTCTC 2 cut(s) 291, 1361
Alw44I GTGCAC 1 cut(s) 489
AlwI GGATC 1 cut(s) 1445
AlwNI CAGNNNCTG 1 cut(s) 1700
Ama87I CYCGRG 1 cut(s) 1464
AoxI GGCC 3 cut(s) 401, 888, 1181
ApaLI GTGCAC 1 cut(s) 489
ApeKI GCWGC 6 cut(s) 307, 502, 878, 986, 1216, 1450
ApoI RAATTY 3 cut(s) 121, 1106, 1124
Asp700I GAANNNNTTC 1 cut(s) 708
AspLEI GCGC 4 cut(s) 12, 483, 485, 507
AspS9I GGNCC 3 cut(s) 401, 888, 916
AsuHPI GGTGA 7 cut(s) 108, 412, 976, 1070, 1199, 1214, 1315
AvaI CYCGRG 1 cut(s) 1464
AvaII GGWCC 1 cut(s) 916
AxyI CCTNAGG 1 cut(s) 788
BaeGI GKGCMC 2 cut(s) 493, 787
BanII GRGCYC 3 cut(s) 770, 1006, 1524
BauI CACGAG 2 cut(s) 1053, 1517
BbsI GAAGAC 1 cut(s) 1087
Bbv12I GWGCWC 2 cut(s) 493, 1524
BbvI GCAGC 6 cut(s) 319, 514, 890, 998, 1228, 1462
BccI CCATC 5 cut(s) 644, 1055, 1259, 1646, 1665
BciT130I CCWGG 4 cut(s) 179, 476, 1185, 1543
BciVI GTATCC 1 cut(s) 932
BcoDI GTCTC 2 cut(s) 291, 1361
BfaI CTAG 4 cut(s) 447, 678, 1196, 1373
BfmI CTRYAG 3 cut(s) 305, 904, 1214
BfoI RGCGCY 1 cut(s) 508
BfrI CTTAAG 1 cut(s) 271
BfuAI ACCTGC 1 cut(s) 606
BfuI GTATCC 1 cut(s) 932
BglI GCCNNNNNGGC 1 cut(s) 887
BglII AGATCT 2 cut(s) 467, 846
BisI GCNGC 6 cut(s) 308, 503, 879, 987, 1217, 1451
BlsI GCNGC 6 cut(s) 309, 504, 880, 988, 1218, 1452
Bme1390I CCNGG 4 cut(s) 179, 476, 1185, 1543
Bme18I GGWCC 1 cut(s) 916
BmeT110I CYCGRG 1 cut(s) 1464
BmgBI CACGTC 1 cut(s) 1408
BmgT120I GGNCC 3 cut(s) 401, 888, 916
BmiI GGNNCC 6 cut(s) 402, 769, 801, 889, 1005, 1137
BmrFI CCNGG 4 cut(s) 179, 476, 1185, 1543
BmrI ACTGGG 2 cut(s) 990, 1112
BmsI GCATC 4 cut(s) 331, 335, 489, 578
BmuI ACTGGG 2 cut(s) 990, 1112
BpiI GAAGAC 1 cut(s) 1087
BpmI CTGGAG 2 cut(s) 458, 1661
BpuEI CTTGAG 1 cut(s) 933
Bsa29I ATCGAT 1 cut(s) 1029
BsaHI GRCGYC 1 cut(s) 900
BsaJI CCNNGG 2 cut(s) 1259, 1418
BsaWI WCCGGW 1 cut(s) 1162
Bsc4I CCNNNNNNNGG 2 cut(s) 283, 1265
Bse1I ACTGG 4 cut(s) 40, 996, 1118, 1139
Bse21I CCTNAGG 1 cut(s) 788
Bse3DI GCAATG 2 cut(s) 891, 1395
BseBI CCWGG 4 cut(s) 179, 476, 1185, 1543
BseCI ATCGAT 1 cut(s) 1029
BseDI CCNNGG 2 cut(s) 1259, 1418
BseGI GGATG 7 cut(s) 61, 346, 655, 1113, 1270, 1657, 1676
BseLI CCNNNNNNNGG 2 cut(s) 283, 1265
BseMI GCAATG 2 cut(s) 891, 1395
BseMII CTCAG 7 cut(s) 158, 222, 802, 1092, 1265, 1604, 1638
BseNI ACTGG 4 cut(s) 40, 996, 1118, 1139
BsePI GCGCGC 1 cut(s) 481
BseRI GAGGAG 1 cut(s) 1658
BseSI GKGCMC 2 cut(s) 493, 787
BseXI GCAGC 6 cut(s) 319, 514, 890, 998, 1228, 1462
BsgI GTGCAG 2 cut(s) 60, 814
Bsh1236I CGCG 3 cut(s) 10, 483, 485
BshFI GGCC 3 cut(s) 403, 890, 1183
BshVI ATCGAT 1 cut(s) 1029
BsiHKAI GWGCWC 2 cut(s) 493, 1524
BsiHKCI CYCGRG 1 cut(s) 1464
BsiSI CCGG 1 cut(s) 1163
BslFI GGGAC 1 cut(s) 474
BslI CCNNNNNNNGG 2 cut(s) 283, 1265
BsmAI GTCTC 2 cut(s) 291, 1361
BsmFI GGGAC 1 cut(s) 474
BsnI GGCC 3 cut(s) 403, 890, 1183
BsoBI CYCGRG 1 cut(s) 1464
Bsp1286I GDGCHC 5 cut(s) 493, 770, 787, 1006, 1524
Bsp143I GATC 3 cut(s) 467, 846, 1437
BspACI CCGC 3 cut(s) 423, 454, 485
BspANI GGCC 3 cut(s) 403, 890, 1183
BspCNI CTCAG 7 cut(s) 159, 221, 801, 1093, 1266, 1605, 1637
BspDI ATCGAT 1 cut(s) 1029
BspFNI CGCG 3 cut(s) 10, 483, 485
BspLI GGNNCC 6 cut(s) 402, 769, 801, 889, 1005, 1137
BspMAI CTGCAG 3 cut(s) 309, 908, 1218
BspMI ACCTGC 1 cut(s) 606
BspPI GGATC 1 cut(s) 1445
BspTI CTTAAG 1 cut(s) 271
BsrDI GCAATG 2 cut(s) 891, 1395
BsrI ACTGG 4 cut(s) 40, 996, 1118, 1139
BssECI CCNNGG 2 cut(s) 1259, 1418
BssHII GCGCGC 1 cut(s) 481
BssMI GATC 3 cut(s) 467, 846, 1437
BssNI GRCGYC 1 cut(s) 900
BssSI CACGAG 2 cut(s) 1053, 1517
BssT1I CCWWGG 1 cut(s) 1259
Bst2BI CACGAG 2 cut(s) 1053, 1517
Bst2UI CCWGG 4 cut(s) 179, 476, 1185, 1543
Bst4CI ACNGT 7 cut(s) 157, 251, 302, 537, 782, 920, 1086
Bst6I CTCTTC 2 cut(s) 319, 408
BstACI GRCGYC 1 cut(s) 900
BstAFI CTTAAG 1 cut(s) 271
BstC8I GCNNGC 6 cut(s) 14, 43, 452, 483, 904, 1489
BstDEI CTNAG 9 cut(s) 167, 188, 208, 788, 1044, 1101, 1274, 1613, 1624
BstEII GGTNACC 1 cut(s) 418
BstF5I GGATG 7 cut(s) 61, 346, 655, 1113, 1270, 1657, 1676
BstFNI CGCG 3 cut(s) 10, 483, 485
BstH2I RGCGCY 1 cut(s) 508
BstHHI GCGC 4 cut(s) 12, 483, 485, 507
BstKTI GATC 3 cut(s) 470, 849, 1440
BstMAI GTCTC 2 cut(s) 291, 1361
BstMBI GATC 3 cut(s) 467, 846, 1437
BstMWI GCNNNNNNNGC 5 cut(s) 607, 683, 887, 986, 1636
BstNI CCWGG 4 cut(s) 179, 476, 1185, 1543
BstNSI RCATGY 4 cut(s) 497, 621, 832, 1171
BstPI GGTNACC 1 cut(s) 418
BstSCI CCNGG 4 cut(s) 177, 474, 1183, 1541
BstSFI CTRYAG 3 cut(s) 305, 904, 1214
BstSLI GKGCMC 2 cut(s) 493, 787
BstUI CGCG 3 cut(s) 10, 483, 485
BstV1I GCAGC 6 cut(s) 319, 514, 890, 998, 1228, 1462
BstV2I GAAGAC 1 cut(s) 1087
BstX2I RGATCY 2 cut(s) 467, 846
BstXI CCANNNNNNTGG 1 cut(s) 20
BstYI RGATCY 2 cut(s) 467, 846
Bsu15I ATCGAT 1 cut(s) 1029
Bsu36I CCTNAGG 1 cut(s) 788
BsuI GTATCC 1 cut(s) 932
BsuRI GGCC 3 cut(s) 403, 890, 1183
BsuTUI ATCGAT 1 cut(s) 1029
BtgZI GCGATG 1 cut(s) 825
BtrI CACGTC 1 cut(s) 1408
BtsCI GGATG 7 cut(s) 61, 346, 655, 1113, 1270, 1657, 1676
BtsI GCAGTG 2 cut(s) 66, 1206
BtsIMutI CAGTG 6 cut(s) 34, 66, 703, 1003, 1206, 1708
BveI ACCTGC 1 cut(s) 606
Cac8I GCNNGC 6 cut(s) 14, 43, 452, 483, 904, 1489
CaiI CAGNNNCTG 1 cut(s) 1700
CfoI GCGC 4 cut(s) 12, 483, 485, 507
Cfr13I GGNCC 3 cut(s) 401, 888, 916
ClaI ATCGAT 1 cut(s) 1029
CseI GACGC 2 cut(s) 704, 908
Csp6I GTAC 2 cut(s) 954, 1013
CviAII CATG 7 cut(s) 494, 618, 730, 829, 874, 1010, 1168
CviQI GTAC 2 cut(s) 954, 1013
DdeI CTNAG 9 cut(s) 167, 188, 208, 788, 1044, 1101, 1274, 1613, 1624
DpnI GATC 3 cut(s) 469, 848, 1439
DpnII GATC 3 cut(s) 467, 846, 1437
DraI TTTAAA 1 cut(s) 861
DraIII CACNNNGTG 1 cut(s) 556
Eam1104I CTCTTC 2 cut(s) 319, 408
EarI CTCTTC 2 cut(s) 319, 408
EciI GGCGGA 1 cut(s) 469
Ecl136II GAGCTC 1 cut(s) 1522
Eco130I CCWWGG 1 cut(s) 1259
Eco147I AGGCCT 1 cut(s) 1183
Eco24I GRGCYC 3 cut(s) 770, 1006, 1524
Eco47I GGWCC 1 cut(s) 916
Eco53kI GAGCTC 1 cut(s) 1522
Eco57I CTGAAG 1 cut(s) 645
Eco81I CCTNAGG 1 cut(s) 788
Eco88I CYCGRG 1 cut(s) 1464
Eco91I GGTNACC 1 cut(s) 418
EcoICRI GAGCTC 1 cut(s) 1522
EcoO109I RGGNCCY 1 cut(s) 401
EcoO65I GGTNACC 1 cut(s) 418
EcoRII CCWGG 4 cut(s) 177, 474, 1183, 1541
EcoT14I CCWWGG 1 cut(s) 1259
EcoT38I GRGCYC 3 cut(s) 770, 1006, 1524
ErhI CCWWGG 1 cut(s) 1259
FaeI CATG 7 cut(s) 497, 621, 733, 832, 877, 1013, 1171
FaqI GGGAC 1 cut(s) 474
FatI CATG 7 cut(s) 493, 617, 729, 828, 873, 1009, 1167
FauI CCCGC 1 cut(s) 478
FauNDI CATATG 1 cut(s) 1585
FblI GTMKAC 1 cut(s) 1609
Fnu4HI GCNGC 6 cut(s) 308, 503, 879, 987, 1217, 1451
FokI GGATG 7 cut(s) 48, 353, 662, 1100, 1277, 1664, 1683
FriOI GRGCYC 3 cut(s) 770, 1006, 1524
Fsp4HI GCNGC 6 cut(s) 308, 503, 879, 987, 1217, 1451
FspBI CTAG 4 cut(s) 447, 678, 1196, 1373
GlaI GCGC 4 cut(s) 11, 482, 484, 506
GluI GCNGC 6 cut(s) 308, 503, 879, 987, 1217, 1451
GsuI CTGGAG 2 cut(s) 458, 1661
HaeII RGCGCY 1 cut(s) 508
HaeIII GGCC 3 cut(s) 403, 890, 1183
HapII CCGG 1 cut(s) 1163
HgaI GACGC 2 cut(s) 704, 908
HhaI GCGC 4 cut(s) 12, 483, 485, 507
Hin1I GRCGYC 1 cut(s) 900
Hin1II CATG 7 cut(s) 497, 621, 733, 832, 877, 1013, 1171
Hin6I GCGC 4 cut(s) 10, 481, 483, 505
HinP1I GCGC 4 cut(s) 10, 481, 483, 505
HincII GTYRAC 2 cut(s) 1548, 1591
HindII GTYRAC 2 cut(s) 1548, 1591
HindIII AAGCTT 1 cut(s) 268
HpaII CCGG 1 cut(s) 1163
HphI GGTGA 7 cut(s) 108, 412, 976, 1070, 1199, 1214, 1315
Hpy166II GTNNAC 6 cut(s) 491, 1204, 1252, 1548, 1591, 1610
Hpy188I TCNGA 6 cut(s) 151, 541, 637, 1102, 1275, 1627
Hpy188III TCNNGA 6 cut(s) 210, 296, 940, 1351, 1373, 1466
Hpy8I GTNNAC 6 cut(s) 491, 1204, 1252, 1548, 1591, 1610
Hpy99I CGWCG 1 cut(s) 297
HpyAV CCTTC 8 cut(s) 288, 451, 731, 748, 983, 1487, 1642, 1669
HpyCH4III ACNGT 7 cut(s) 157, 251, 302, 537, 782, 920, 1086
HpyCH4IV ACGT 3 cut(s) 547, 1407, 1443
HpyF10VI GCNNNNNNNGC 5 cut(s) 607, 683, 887, 986, 1636
HpyF3I CTNAG 9 cut(s) 167, 188, 208, 788, 1044, 1101, 1274, 1613, 1624
HpySE526I ACGT 3 cut(s) 547, 1407, 1443
Hsp92I GRCGYC 1 cut(s) 900
Hsp92II CATG 7 cut(s) 497, 621, 733, 832, 877, 1013, 1171
HspAI GCGC 4 cut(s) 10, 481, 483, 505
Kzo9I GATC 3 cut(s) 467, 846, 1437
LmnI GCTCC 9 cut(s) 76, 181, 197, 478, 722, 773, 799, 1009, 1447
Lsp1109I GCAGC 6 cut(s) 319, 514, 890, 998, 1228, 1462
LweI GCATC 4 cut(s) 331, 335, 489, 578
MaeI CTAG 4 cut(s) 447, 678, 1196, 1373
MaeII ACGT 3 cut(s) 547, 1407, 1443
MalI GATC 3 cut(s) 469, 848, 1439
MboI GATC 3 cut(s) 467, 846, 1437
MboII GAAGA 4 cut(s) 306, 422, 425, 1092
MfeI CAATTG 1 cut(s) 605
MflI RGATCY 2 cut(s) 467, 846
MhlI GDGCHC 5 cut(s) 493, 770, 787, 1006, 1524
MlyI GAGTC 6 cut(s) 222, 961, 1363, 1405, 1406, 1615
MroXI GAANNNNTTC 1 cut(s) 708
MseI TTAA 6 cut(s) 266, 272, 395, 860, 1110, 1577
MspCI CTTAAG 1 cut(s) 271
MspI CCGG 1 cut(s) 1163
MspR9I CCNGG 4 cut(s) 179, 476, 1185, 1543
MunI CAATTG 1 cut(s) 605
MvaI CCWGG 4 cut(s) 179, 476, 1185, 1543
MvnI CGCG 3 cut(s) 10, 483, 485
MwoI GCNNNNNNNGC 5 cut(s) 607, 683, 887, 986, 1636
NdeI CATATG 1 cut(s) 1585
NdeII GATC 3 cut(s) 467, 846, 1437
NlaIII CATG 7 cut(s) 497, 621, 733, 832, 877, 1013, 1171
NlaIV GGNNCC 6 cut(s) 402, 769, 801, 889, 1005, 1137
NmeAIII GCCGAG 1 cut(s) 1443
NmuCI GTSAC 8 cut(s) 170, 418, 548, 964, 1156, 1287, 1303, 1408
NspI RCATGY 4 cut(s) 497, 621, 832, 1171
PaeR7I CTCGAG 1 cut(s) 1464
PauI GCGCGC 1 cut(s) 481
PceI AGGCCT 1 cut(s) 1183
PciI ACATGT 1 cut(s) 493
PdmI GAANNNNTTC 1 cut(s) 708
PfeI GAWTC 3 cut(s) 146, 1325, 1354
PflMI CCANNNNNTGG 1 cut(s) 1265
PfoI TCCNGGA 1 cut(s) 474
PkrI GCNGC 6 cut(s) 309, 504, 880, 988, 1218, 1452
PleI GAGTC 6 cut(s) 221, 961, 1363, 1405, 1405, 1614
PpsI GAGTC 6 cut(s) 221, 961, 1363, 1405, 1405, 1614
PscI ACATGT 1 cut(s) 493
PsiI TTATAA 1 cut(s) 1635
Psp124BI GAGCTC 1 cut(s) 1524
Psp6I CCWGG 4 cut(s) 177, 474, 1183, 1541
PspEI GGTNACC 1 cut(s) 418
PspGI CCWGG 4 cut(s) 177, 474, 1183, 1541
PspN4I GGNNCC 6 cut(s) 402, 769, 801, 889, 1005, 1137
PspPI GGNCC 3 cut(s) 401, 888, 916
PstI CTGCAG 3 cut(s) 309, 908, 1218
PstNI CAGNNNCTG 1 cut(s) 1700
PsuI RGATCY 2 cut(s) 467, 846
PteI GCGCGC 1 cut(s) 481
RsaI GTAC 2 cut(s) 955, 1014
RsaNI GTAC 2 cut(s) 954, 1013
SacI GAGCTC 1 cut(s) 1524
SaqAI TTAA 6 cut(s) 266, 272, 395, 860, 1110, 1577
SatI GCNGC 6 cut(s) 308, 503, 879, 987, 1217, 1451
Sau3AI GATC 3 cut(s) 467, 846, 1437
Sau96I GGNCC 3 cut(s) 401, 888, 916
SchI GAGTC 6 cut(s) 222, 961, 1363, 1405, 1406, 1615
ScrFI CCNGG 4 cut(s) 179, 476, 1185, 1543
SduI GDGCHC 5 cut(s) 493, 770, 787, 1006, 1524
SfaNI GCATC 4 cut(s) 331, 335, 489, 578
SfcI CTRYAG 3 cut(s) 305, 904, 1214
Sfr274I CTCGAG 1 cut(s) 1464
SinI GGWCC 1 cut(s) 916
SlaI CTCGAG 1 cut(s) 1464
SmlI CTYRAG 3 cut(s) 271, 948, 1464
SmoI CTYRAG 3 cut(s) 271, 948, 1464
SseBI AGGCCT 1 cut(s) 1183
SsiI CCGC 3 cut(s) 423, 454, 485
SspMI CTAG 4 cut(s) 447, 678, 1196, 1373
SstI GAGCTC 1 cut(s) 1524
StuI AGGCCT 1 cut(s) 1183
StyD4I CCNGG 4 cut(s) 177, 474, 1183, 1541
StyI CCWWGG 1 cut(s) 1259
TaaI ACNGT 7 cut(s) 157, 251, 302, 537, 782, 920, 1086
TaiI ACGT 3 cut(s) 550, 1410, 1446
TaqI TCGA 3 cut(s) 295, 1029, 1465
TfiI GAWTC 3 cut(s) 146, 1325, 1354
Tru1I TTAA 6 cut(s) 266, 272, 395, 860, 1110, 1577
Tru9I TTAA 6 cut(s) 266, 272, 395, 860, 1110, 1577
TscAI CASTG 5 cut(s) 34, 73, 703, 1003, 1206
TseFI GTSAC 8 cut(s) 170, 418, 548, 964, 1156, 1287, 1303, 1408
TseI GCWGC 6 cut(s) 307, 502, 878, 986, 1216, 1450
Tsp45I GTSAC 8 cut(s) 170, 418, 548, 964, 1156, 1287, 1303, 1408
TspDTI ATGAA 3 cut(s) 423, 579, 1251
TspRI CASTG 5 cut(s) 34, 73, 703, 1003, 1206
Van91I CCANNNNNTGG 1 cut(s) 1265
Vha464I CTTAAG 1 cut(s) 271
VneI GTGCAC 1 cut(s) 489
VpaK11BI GGWCC 1 cut(s) 916
XapI RAATTY 3 cut(s) 121, 1106, 1124
XbaI TCTAGA 1 cut(s) 1372
XceI RCATGY 4 cut(s) 497, 621, 832, 1171
XcmI CCANNNNNNNNNTGG 2 cut(s) 1523, 1549
XhoI CTCGAG 1 cut(s) 1464
XmiI GTMKAC 1 cut(s) 1609
XmnI GAANNNNTTC 1 cut(s) 708
XspI CTAG 4 cut(s) 447, 678, 1196, 1373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.