Rw5G023220

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0013641
Physical Location & Seq
Reverse (-)
32690831 .. 32694096
3266 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G023220.1

Sequence Viewer

Length: 1722 bp
ATGGACTCGCGCCAGCGTTTTGGTTTCAGTGAAACTGGTGCAGGCTATTTTGGAGGTTCTCATCCCACTGCTCCTAACAGGTTGCTTGGGTCTTTGAAATTAGATATTGGTAACTCACCAAATTCACACTTTTCAACTCACTTTGATTCTGATACTGTTACTACGCTGAGTGACTGCCAGGAGCAACTTAGCTCCACAGAAAATCTCTCAGGAGTCAGCCATTCCTCTAACTCGCCATTTGAAACCAATAGTTATATTTCTCGGTTAAGCTTAAGCCCTTCTGTGGATTGTCGTCGAGACAGTCTGCAGCTCTCTTCTGGTAGGGCATCTTTGTTACAGGATGCAAATTGTAGCCCCAATATAAAATACGCTTTGCAGGAATTGGAGAGTGCTTTAATGGGGCCTGATGAAGAAGAGGTCACCGCAACAAACATTTTTGGGGAAACTAGCAGGCGGAAGGGACAGAGATCTCTGTCATGGAACCAGGAGCGCGCGGGTGCACATGTGATGCAGCGCCAGACTTCCTTTGTTTCAGGGCATAGACAGTCAGAGAAACGTCACAAGGTGCTTGATGAAGCATCTCCACAGGGTTTTCCGGCTGATAACCTGCGACATTTGCTGATTGCATGTGCTGAAGCACTTTCTGACAACAATATGGATGGTTTTGAGAAGTTGATTGAAAAGGCTAGAGGTGCTGTGTCTATCAGTGGAGAACCAATGCAGCGTCTTGGAGCTTACATGGTAGAAGGGCTGGTTGCAAGGAAGGAGGCTTCGGGCTCCAATATCTACCGTGCCCTCAGGTGCAGGGAGCCTGAAAGCGATGACTTGCTCTCATACATGCACATTCTGTATGAGATCTGCCCCTATTTAAAGTTCGGTTACATGGCAGCCAATGGGGCCATTGCTGACGCCTGCAGAAATGAGGACCGTATCCACATTATAGACTTCCAGATTGCTCAAGGTACTCAATGGGTGACTCTCCTTCAAGCTCTTGCAGCAAGACCCAGTGGGGCTCCCCATGTACGAATTACAGGTATCGATGACCCTGTTTCTAAATATGCTCGTGGTGATGGCTTGGAGGCGGTAGGAAGACGGTTGAAAGCAATCTCTGAGAAATTTAACATCCCTGTTGAATTTCACGGGGTTCCAGTTTTTGCACCTGATGTCACACCGGACATGCTTGATGTCAGGCCTGGTGAGGCTCTAGCAGTGAACTTTCCCCTGCAGCTTCACCACACACCAGATGAAAGCGTTGATGTGAACAATCCAAGGGATGGGCTTCTGAGGATAGTCAAGTCACTATCTCCAAAGGTGACTACTTTGGTTGAGCAAGAATCAAACACGAATACAACCCCTTTCTTGAATCGGTTTATAGAGACTCTAGACTACTATTTGGCAATGTTTGAGTCTATTGACGTGACTCTGCCGAGGAACAGCAAGGAGAGGATCAACGTGGAGCAGCATTGTCTGGCTCGAGATATTGTAAATGTGATTGCCTGCGAAGGAAAGGAAAGGGTGGAACGCCACGAGCTCTTTGGGAAGTGGAAGTCCAGGTTGACAATGGCAGGATTTCGGCAATACCCATTAAGCTCATATGTCAACTCTGTCATAAGGAGTCTACTGAGGTGTTACTCAGAGCATTATAAGCTGGAGGAGAAGGATGGTGCTATGCTGTTGGGATGGAAGGAGAGGAACTTGGTATCAGCTTCTGCGTGGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

573

Amino Acids

63.88

Weight (kDa)

6.01

Isoelectric Point (pI)

51.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 203 - 572 2.4e-136 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1644
Acc36I ACCTGC 1 cut(s) 615
AccB7I CCANNNNNTGG 1 cut(s) 1274
AccI GTMKAC 1 cut(s) 1618
AccII CGCG 3 cut(s) 10, 492, 494
AciI CCGC 4 cut(s) 423, 454, 494, 1082
AclWI GGATC 1 cut(s) 1454
AcsI RAATTY 3 cut(s) 121, 1115, 1133
AcuI CTGAAG 1 cut(s) 654
AcyI GRCGYC 1 cut(s) 909
AdeI CACNNNGTG 1 cut(s) 565
AfaI GTAC 2 cut(s) 964, 1023
AfiI CCNNNNNNNGG 2 cut(s) 283, 1274
AflII CTTAAG 1 cut(s) 271
AflIII ACRYGT 1 cut(s) 502
AgsI TTSAA 8 cut(s) 97, 135, 242, 680, 986, 1099, 1133, 1363
AjiI CACGTC 1 cut(s) 1417
AjnI CCWGG 4 cut(s) 177, 483, 1192, 1550
Alw21I GWGCWC 2 cut(s) 502, 1533
Alw26I GTCTC 2 cut(s) 291, 1370
Alw44I GTGCAC 1 cut(s) 498
AlwI GGATC 1 cut(s) 1454
AlwNI CAGNNNCTG 1 cut(s) 1709
Ama87I CYCGRG 1 cut(s) 1473
AoxI GGCC 3 cut(s) 401, 897, 1190
ApaLI GTGCAC 1 cut(s) 498
ApeKI GCWGC 7 cut(s) 307, 511, 721, 887, 995, 1225, 1459
ApoI RAATTY 3 cut(s) 121, 1115, 1133
AspLEI GCGC 4 cut(s) 12, 492, 494, 516
AspS9I GGNCC 3 cut(s) 401, 897, 925
AsuHPI GGTGA 7 cut(s) 108, 412, 985, 1079, 1208, 1223, 1324
AvaI CYCGRG 1 cut(s) 1473
AvaII GGWCC 1 cut(s) 925
AxyI CCTNAGG 1 cut(s) 797
BaeGI GKGCMC 2 cut(s) 502, 796
BanII GRGCYC 3 cut(s) 779, 1015, 1533
BauI CACGAG 2 cut(s) 1062, 1526
BbsI GAAGAC 1 cut(s) 1096
Bbv12I GWGCWC 2 cut(s) 502, 1533
BbvI GCAGC 7 cut(s) 319, 523, 733, 899, 1007, 1237, 1471
BccI CCATC 5 cut(s) 653, 1064, 1268, 1655, 1674
BciT130I CCWGG 4 cut(s) 179, 485, 1194, 1552
BciVI GTATCC 1 cut(s) 941
BcoDI GTCTC 2 cut(s) 291, 1370
BfaI CTAG 4 cut(s) 447, 687, 1205, 1382
BfmI CTRYAG 3 cut(s) 305, 913, 1223
BfoI RGCGCY 1 cut(s) 517
BfrI CTTAAG 1 cut(s) 271
BfuAI ACCTGC 1 cut(s) 615
BfuI GTATCC 1 cut(s) 941
BglI GCCNNNNNGGC 1 cut(s) 896
BglII AGATCT 2 cut(s) 467, 855
BisI GCNGC 7 cut(s) 308, 512, 722, 888, 996, 1226, 1460
BlsI GCNGC 7 cut(s) 309, 513, 723, 889, 997, 1227, 1461
Bme1390I CCNGG 4 cut(s) 179, 485, 1194, 1552
Bme18I GGWCC 1 cut(s) 925
BmeT110I CYCGRG 1 cut(s) 1473
BmgBI CACGTC 1 cut(s) 1417
BmgT120I GGNCC 3 cut(s) 401, 897, 925
BmiI GGNNCC 7 cut(s) 402, 482, 778, 810, 898, 1014, 1146
BmrFI CCNGG 4 cut(s) 179, 485, 1194, 1552
BmrI ACTGGG 1 cut(s) 999
BmsI GCATC 4 cut(s) 331, 335, 498, 587
BmuI ACTGGG 1 cut(s) 999
BpiI GAAGAC 1 cut(s) 1096
BpmI CTGGAG 1 cut(s) 1670
BpuEI CTTGAG 1 cut(s) 942
Bsa29I ATCGAT 1 cut(s) 1038
BsaHI GRCGYC 1 cut(s) 909
BsaJI CCNNGG 2 cut(s) 1268, 1427
BsaWI WCCGGW 1 cut(s) 1171
Bsc4I CCNNNNNNNGG 2 cut(s) 283, 1274
Bse1I ACTGG 3 cut(s) 40, 1005, 1148
Bse21I CCTNAGG 1 cut(s) 797
Bse3DI GCAATG 2 cut(s) 900, 1404
BseBI CCWGG 4 cut(s) 179, 485, 1194, 1552
BseCI ATCGAT 1 cut(s) 1038
BseDI CCNNGG 2 cut(s) 1268, 1427
BseGI GGATG 7 cut(s) 61, 346, 664, 1122, 1279, 1666, 1685
BseLI CCNNNNNNNGG 2 cut(s) 283, 1274
BseMI GCAATG 2 cut(s) 900, 1404
BseMII CTCAG 7 cut(s) 158, 222, 811, 1101, 1274, 1613, 1647
BseNI ACTGG 3 cut(s) 40, 1005, 1148
BsePI GCGCGC 1 cut(s) 490
BseRI GAGGAG 1 cut(s) 1667
BseSI GKGCMC 2 cut(s) 502, 796
BseXI GCAGC 7 cut(s) 319, 523, 733, 899, 1007, 1237, 1471
BsgI GTGCAG 2 cut(s) 60, 823
Bsh1236I CGCG 3 cut(s) 10, 492, 494
BshFI GGCC 3 cut(s) 403, 899, 1192
BshVI ATCGAT 1 cut(s) 1038
BsiHKAI GWGCWC 2 cut(s) 502, 1533
BsiHKCI CYCGRG 1 cut(s) 1473
BsiSI CCGG 2 cut(s) 596, 1172
BslFI GGGAC 1 cut(s) 474
BslI CCNNNNNNNGG 2 cut(s) 283, 1274
BsmAI GTCTC 2 cut(s) 291, 1370
BsmFI GGGAC 1 cut(s) 474
BsnI GGCC 3 cut(s) 403, 899, 1192
BsoBI CYCGRG 1 cut(s) 1473
Bsp1286I GDGCHC 5 cut(s) 502, 779, 796, 1015, 1533
Bsp143I GATC 3 cut(s) 467, 855, 1446
BspACI CCGC 4 cut(s) 423, 454, 494, 1082
BspANI GGCC 3 cut(s) 403, 899, 1192
BspCNI CTCAG 7 cut(s) 159, 221, 810, 1102, 1275, 1614, 1646
BspDI ATCGAT 1 cut(s) 1038
BspFNI CGCG 3 cut(s) 10, 492, 494
BspLI GGNNCC 7 cut(s) 402, 482, 778, 810, 898, 1014, 1146
BspMAI CTGCAG 3 cut(s) 309, 917, 1227
BspMI ACCTGC 1 cut(s) 615
BspPI GGATC 1 cut(s) 1454
BspTI CTTAAG 1 cut(s) 271
BsrDI GCAATG 2 cut(s) 900, 1404
BsrI ACTGG 3 cut(s) 40, 1005, 1148
BssECI CCNNGG 2 cut(s) 1268, 1427
BssHII GCGCGC 1 cut(s) 490
BssMI GATC 3 cut(s) 467, 855, 1446
BssNI GRCGYC 1 cut(s) 909
BssSI CACGAG 2 cut(s) 1062, 1526
BssT1I CCWWGG 1 cut(s) 1268
Bst2BI CACGAG 2 cut(s) 1062, 1526
Bst2UI CCWGG 4 cut(s) 179, 485, 1194, 1552
Bst4CI ACNGT 6 cut(s) 157, 302, 546, 791, 929, 1095
Bst6I CTCTTC 2 cut(s) 319, 408
BstACI GRCGYC 1 cut(s) 909
BstAFI CTTAAG 1 cut(s) 271
BstC8I GCNNGC 6 cut(s) 14, 43, 452, 492, 913, 1498
BstDEI CTNAG 8 cut(s) 167, 188, 208, 797, 1110, 1283, 1622, 1633
BstEII GGTNACC 1 cut(s) 418
BstF5I GGATG 7 cut(s) 61, 346, 664, 1122, 1279, 1666, 1685
BstFNI CGCG 3 cut(s) 10, 492, 494
BstH2I RGCGCY 1 cut(s) 517
BstHHI GCGC 4 cut(s) 12, 492, 494, 516
BstKTI GATC 3 cut(s) 470, 858, 1449
BstMAI GTCTC 2 cut(s) 291, 1370
BstMBI GATC 3 cut(s) 467, 855, 1446
BstMWI GCNNNNNNNGC 5 cut(s) 616, 692, 896, 995, 1645
BstNI CCWGG 4 cut(s) 179, 485, 1194, 1552
BstNSI RCATGY 4 cut(s) 506, 630, 841, 1180
BstPI GGTNACC 1 cut(s) 418
BstSCI CCNGG 4 cut(s) 177, 483, 1192, 1550
BstSFI CTRYAG 3 cut(s) 305, 913, 1223
BstSLI GKGCMC 2 cut(s) 502, 796
BstUI CGCG 3 cut(s) 10, 492, 494
BstV1I GCAGC 7 cut(s) 319, 523, 733, 899, 1007, 1237, 1471
BstV2I GAAGAC 1 cut(s) 1096
BstX2I RGATCY 2 cut(s) 467, 855
BstXI CCANNNNNNTGG 1 cut(s) 20
BstYI RGATCY 2 cut(s) 467, 855
Bsu15I ATCGAT 1 cut(s) 1038
Bsu36I CCTNAGG 1 cut(s) 797
BsuI GTATCC 1 cut(s) 941
BsuRI GGCC 3 cut(s) 403, 899, 1192
BsuTUI ATCGAT 1 cut(s) 1038
BtgZI GCGATG 1 cut(s) 834
BtrI CACGTC 1 cut(s) 1417
BtsCI GGATG 7 cut(s) 61, 346, 664, 1122, 1279, 1666, 1685
BtsI GCAGTG 2 cut(s) 66, 1215
BtsIMutI CAGTG 6 cut(s) 34, 66, 712, 1012, 1215, 1717
BveI ACCTGC 1 cut(s) 615
Cac8I GCNNGC 6 cut(s) 14, 43, 452, 492, 913, 1498
CaiI CAGNNNCTG 1 cut(s) 1709
CfoI GCGC 4 cut(s) 12, 492, 494, 516
Cfr13I GGNCC 3 cut(s) 401, 897, 925
ClaI ATCGAT 1 cut(s) 1038
CseI GACGC 2 cut(s) 713, 917
Csp6I GTAC 2 cut(s) 963, 1022
CviAII CATG 8 cut(s) 477, 503, 627, 739, 838, 883, 1019, 1177
CviQI GTAC 2 cut(s) 963, 1022
DdeI CTNAG 8 cut(s) 167, 188, 208, 797, 1110, 1283, 1622, 1633
DpnI GATC 3 cut(s) 469, 857, 1448
DpnII GATC 3 cut(s) 467, 855, 1446
DraI TTTAAA 1 cut(s) 870
DraIII CACNNNGTG 1 cut(s) 565
Eam1104I CTCTTC 2 cut(s) 319, 408
EarI CTCTTC 2 cut(s) 319, 408
EciI GGCGGA 1 cut(s) 469
Ecl136II GAGCTC 1 cut(s) 1531
Eco130I CCWWGG 1 cut(s) 1268
Eco147I AGGCCT 1 cut(s) 1192
Eco24I GRGCYC 3 cut(s) 779, 1015, 1533
Eco47I GGWCC 1 cut(s) 925
Eco53kI GAGCTC 1 cut(s) 1531
Eco57I CTGAAG 1 cut(s) 654
Eco81I CCTNAGG 1 cut(s) 797
Eco88I CYCGRG 1 cut(s) 1473
Eco91I GGTNACC 1 cut(s) 418
EcoICRI GAGCTC 1 cut(s) 1531
EcoO109I RGGNCCY 1 cut(s) 401
EcoO65I GGTNACC 1 cut(s) 418
EcoRII CCWGG 4 cut(s) 177, 483, 1192, 1550
EcoT14I CCWWGG 1 cut(s) 1268
EcoT38I GRGCYC 3 cut(s) 779, 1015, 1533
ErhI CCWWGG 1 cut(s) 1268
FaeI CATG 8 cut(s) 480, 506, 630, 742, 841, 886, 1022, 1180
FaqI GGGAC 1 cut(s) 474
FatI CATG 8 cut(s) 476, 502, 626, 738, 837, 882, 1018, 1176
FauI CCCGC 1 cut(s) 487
FauNDI CATATG 1 cut(s) 1594
FblI GTMKAC 1 cut(s) 1618
Fnu4HI GCNGC 7 cut(s) 308, 512, 722, 888, 996, 1226, 1460
FokI GGATG 7 cut(s) 48, 353, 671, 1109, 1286, 1673, 1692
FriOI GRGCYC 3 cut(s) 779, 1015, 1533
Fsp4HI GCNGC 7 cut(s) 308, 512, 722, 888, 996, 1226, 1460
FspBI CTAG 4 cut(s) 447, 687, 1205, 1382
GlaI GCGC 4 cut(s) 11, 491, 493, 515
GluI GCNGC 7 cut(s) 308, 512, 722, 888, 996, 1226, 1460
GsuI CTGGAG 1 cut(s) 1670
HaeII RGCGCY 1 cut(s) 517
HaeIII GGCC 3 cut(s) 403, 899, 1192
HapII CCGG 2 cut(s) 596, 1172
HgaI GACGC 2 cut(s) 713, 917
HhaI GCGC 4 cut(s) 12, 492, 494, 516
Hin1I GRCGYC 1 cut(s) 909
Hin1II CATG 8 cut(s) 480, 506, 630, 742, 841, 886, 1022, 1180
Hin6I GCGC 4 cut(s) 10, 490, 492, 514
HinP1I GCGC 4 cut(s) 10, 490, 492, 514
HincII GTYRAC 2 cut(s) 1557, 1600
HindII GTYRAC 2 cut(s) 1557, 1600
HindIII AAGCTT 1 cut(s) 268
HpaII CCGG 2 cut(s) 596, 1172
HphI GGTGA 7 cut(s) 108, 412, 985, 1079, 1208, 1223, 1324
Hpy166II GTNNAC 6 cut(s) 500, 1213, 1261, 1557, 1600, 1619
Hpy188I TCNGA 6 cut(s) 151, 550, 646, 1111, 1284, 1636
Hpy188III TCNNGA 6 cut(s) 210, 296, 949, 1360, 1382, 1475
Hpy8I GTNNAC 6 cut(s) 500, 1213, 1261, 1557, 1600, 1619
Hpy99I CGWCG 1 cut(s) 297
HpyAV CCTTC 8 cut(s) 288, 451, 740, 757, 992, 1496, 1651, 1678
HpyCH4III ACNGT 6 cut(s) 157, 302, 546, 791, 929, 1095
HpyCH4IV ACGT 3 cut(s) 556, 1416, 1452
HpyF10VI GCNNNNNNNGC 5 cut(s) 616, 692, 896, 995, 1645
HpyF3I CTNAG 8 cut(s) 167, 188, 208, 797, 1110, 1283, 1622, 1633
HpySE526I ACGT 3 cut(s) 556, 1416, 1452
Hsp92I GRCGYC 1 cut(s) 909
Hsp92II CATG 8 cut(s) 480, 506, 630, 742, 841, 886, 1022, 1180
HspAI GCGC 4 cut(s) 10, 490, 492, 514
Kzo9I GATC 3 cut(s) 467, 855, 1446
LmnI GCTCC 9 cut(s) 76, 181, 197, 487, 731, 782, 808, 1018, 1456
Lsp1109I GCAGC 7 cut(s) 319, 523, 733, 899, 1007, 1237, 1471
LweI GCATC 4 cut(s) 331, 335, 498, 587
MaeI CTAG 4 cut(s) 447, 687, 1205, 1382
MaeII ACGT 3 cut(s) 556, 1416, 1452
MalI GATC 3 cut(s) 469, 857, 1448
MboI GATC 3 cut(s) 467, 855, 1446
MboII GAAGA 4 cut(s) 306, 422, 425, 1101
MflI RGATCY 2 cut(s) 467, 855
MhlI GDGCHC 5 cut(s) 502, 779, 796, 1015, 1533
MluCI AATT 7 cut(s) 98, 121, 346, 380, 1026, 1115, 1133
MlyI GAGTC 6 cut(s) 222, 970, 1372, 1414, 1415, 1624
MseI TTAA 6 cut(s) 266, 272, 395, 869, 1119, 1586
MspCI CTTAAG 1 cut(s) 271
MspI CCGG 2 cut(s) 596, 1172
MspR9I CCNGG 4 cut(s) 179, 485, 1194, 1552
MvaI CCWGG 4 cut(s) 179, 485, 1194, 1552
MvnI CGCG 3 cut(s) 10, 492, 494
MwoI GCNNNNNNNGC 5 cut(s) 616, 692, 896, 995, 1645
NdeI CATATG 1 cut(s) 1594
NdeII GATC 3 cut(s) 467, 855, 1446
NlaIII CATG 8 cut(s) 480, 506, 630, 742, 841, 886, 1022, 1180
NlaIV GGNNCC 7 cut(s) 402, 482, 778, 810, 898, 1014, 1146
NmeAIII GCCGAG 1 cut(s) 1452
NmuCI GTSAC 8 cut(s) 170, 418, 557, 973, 1165, 1296, 1312, 1417
NspI RCATGY 4 cut(s) 506, 630, 841, 1180
PaeR7I CTCGAG 1 cut(s) 1473
PauI GCGCGC 1 cut(s) 490
PceI AGGCCT 1 cut(s) 1192
PciI ACATGT 1 cut(s) 502
PfeI GAWTC 3 cut(s) 146, 1334, 1363
PflMI CCANNNNNTGG 1 cut(s) 1274
PkrI GCNGC 7 cut(s) 309, 513, 723, 889, 997, 1227, 1461
PleI GAGTC 6 cut(s) 221, 970, 1372, 1414, 1414, 1623
PpsI GAGTC 6 cut(s) 221, 970, 1372, 1414, 1414, 1623
PscI ACATGT 1 cut(s) 502
PsiI TTATAA 1 cut(s) 1644
Psp124BI GAGCTC 1 cut(s) 1533
Psp6I CCWGG 4 cut(s) 177, 483, 1192, 1550
PspEI GGTNACC 1 cut(s) 418
PspGI CCWGG 4 cut(s) 177, 483, 1192, 1550
PspN4I GGNNCC 7 cut(s) 402, 482, 778, 810, 898, 1014, 1146
PspPI GGNCC 3 cut(s) 401, 897, 925
PstI CTGCAG 3 cut(s) 309, 917, 1227
PstNI CAGNNNCTG 1 cut(s) 1709
PsuI RGATCY 2 cut(s) 467, 855
PteI GCGCGC 1 cut(s) 490
RsaI GTAC 2 cut(s) 964, 1023
RsaNI GTAC 2 cut(s) 963, 1022
SacI GAGCTC 1 cut(s) 1533
SaqAI TTAA 6 cut(s) 266, 272, 395, 869, 1119, 1586
SatI GCNGC 7 cut(s) 308, 512, 722, 888, 996, 1226, 1460
Sau3AI GATC 3 cut(s) 467, 855, 1446
Sau96I GGNCC 3 cut(s) 401, 897, 925
SchI GAGTC 6 cut(s) 222, 970, 1372, 1414, 1415, 1624
ScrFI CCNGG 4 cut(s) 179, 485, 1194, 1552
SduI GDGCHC 5 cut(s) 502, 779, 796, 1015, 1533
SfaNI GCATC 4 cut(s) 331, 335, 498, 587
SfcI CTRYAG 3 cut(s) 305, 913, 1223
Sfr274I CTCGAG 1 cut(s) 1473
SinI GGWCC 1 cut(s) 925
SlaI CTCGAG 1 cut(s) 1473
SmlI CTYRAG 3 cut(s) 271, 957, 1473
SmoI CTYRAG 3 cut(s) 271, 957, 1473
Sse9I AATT 7 cut(s) 98, 121, 346, 380, 1026, 1115, 1133
SseBI AGGCCT 1 cut(s) 1192
SsiI CCGC 4 cut(s) 423, 454, 494, 1082
SspMI CTAG 4 cut(s) 447, 687, 1205, 1382
SstI GAGCTC 1 cut(s) 1533
StuI AGGCCT 1 cut(s) 1192
StyD4I CCNGG 4 cut(s) 177, 483, 1192, 1550
StyI CCWWGG 1 cut(s) 1268
TaaI ACNGT 6 cut(s) 157, 302, 546, 791, 929, 1095
TaiI ACGT 3 cut(s) 559, 1419, 1455
TaqI TCGA 3 cut(s) 295, 1038, 1474
TasI AATT 7 cut(s) 98, 121, 346, 380, 1026, 1115, 1133
TfiI GAWTC 3 cut(s) 146, 1334, 1363
Tru1I TTAA 6 cut(s) 266, 272, 395, 869, 1119, 1586
Tru9I TTAA 6 cut(s) 266, 272, 395, 869, 1119, 1586
TscAI CASTG 5 cut(s) 34, 73, 712, 1012, 1215
TseFI GTSAC 8 cut(s) 170, 418, 557, 973, 1165, 1296, 1312, 1417
TseI GCWGC 7 cut(s) 307, 511, 721, 887, 995, 1225, 1459
Tsp45I GTSAC 8 cut(s) 170, 418, 557, 973, 1165, 1296, 1312, 1417
TspDTI ATGAA 3 cut(s) 423, 588, 1260
TspRI CASTG 5 cut(s) 34, 73, 712, 1012, 1215
Van91I CCANNNNNTGG 1 cut(s) 1274
Vha464I CTTAAG 1 cut(s) 271
VneI GTGCAC 1 cut(s) 498
VpaK11BI GGWCC 1 cut(s) 925
XapI RAATTY 3 cut(s) 121, 1115, 1133
XbaI TCTAGA 1 cut(s) 1381
XceI RCATGY 4 cut(s) 506, 630, 841, 1180
XcmI CCANNNNNNNNNTGG 2 cut(s) 1532, 1558
XhoI CTCGAG 1 cut(s) 1473
XmiI GTMKAC 1 cut(s) 1618
XspI CTAG 4 cut(s) 447, 687, 1205, 1382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.