RLG00000034021

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0019934
Physical Location & Seq
Reverse (-)
35453214 .. 35455821
2608 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034021

Sequence Viewer

Length: 1236 bp
ATGAATTCATCATCAGCAGAAACTGTAGCCAACATCGAAGAACTGCTACAACAGATCCTTGTGCGGCTGCCAGCTCTATCTTTGCTTCGTTTCAAGTGCGTCTCCAAGCACTGGCTCTCTCTTATCTCAACCCCCCAATTCTGTCACCTCCACACCCTCCAAAACCAAAACCCTAAAATCTCCGGTTTCTTCGCTAGCCGTACCTACTGCTCCACTTTCATCCCTCTTCGAAACCTATCTGGGACAACTAATCCTTTTAACGCTCTTAATGATTTCGTCCATGCTGACGACCCAGATTCGTTAGGGTTAGATATTCTTCAGTCCTGCAATGGTCTCTTTCTCTGCCACCATAGAGCTGCAGAGAGAAAAAGTCATCCCGTATATGTTGTCAATCCCACAACCAACCAATTCTCGGTTCTTTCCTCCCCAAGAGTTGGGAAAGATCCATTCTTTATTCGCTATGCTTTGGCTTTTGACCCTTCCAAATCGCCTCATTACAAGGTGGTCTGCCTTAATAAACACCAAATAGACATATATTCATCTGAGACTCGAGCTTGGAAGCTTCTGGAGACTTCTTTCAGAACAGAAGACTGGGTCAGTAGGAGTAGGGGAGGCGGAGTATACTGCAATGGCGCAGTTCATTGGATCAGAGACCACACACAGTTGGTGAATAGGAGAGTAAGAGATGATAGTGATGTGTTGCACTACTATGTTATTGGAGAAGATAGTTTGCGACTTGCCGTTTCTAGTCCTCCTCTGGTTGTAACTGCTCCGTATTCGGATTGCTTGTCGCGGATTCCAGATCATAGATATTTTGGGGAGTCTGGTGGCCATTTGTGTTTGATTGATATTTATTGGGGTAGTAAAAGGAATACTCAATTTTATGTGAAGGAGATGAGGAGGGACTACTCTGGCTGGTTTGTCAAGTACCATGTGGATCTTAATCCGTTAGTCTCATTCTCATGTCGTTTTCGTTTCTTGCTTCTTGGTGTAGTGGAAAATGATGATGAAGATCAGCACCACCAGAATGAGGAGGAGGGTTCTTCTTCTCTTCTGTTGCATGTTCCTGGTACTAGCTACTTTCATCCTCCTCAGTCTAACAAGCATTGGGGCAATTTAGATATTTTGGAGTCTCGTGGTCGATCATTTGCATTCTTCTTGATAGTCATAGACCATGACTTACATGATGGAAATGGGGTGGGACTTTTCTGTGTGGTTTGTCGAGTACCACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

412

Amino Acids

46.9

Weight (kDa)

6.88

Isoelectric Point (pI)

48.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 13 - 48 3e-07 F-box domain
FBA_1 PF07734 95 - 219 2.4e-13 F-box associated beta propeller domain
FBA_3 PF08268 96 - 218 1e-07 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019934)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0041051
rosa_laevigata RLG00000034021
rosa_multiflora Rmu_co8329617.1_g000001 Rmu_sc0002531.1_g000065
rosa_roxburghii Rroxscaffold_1G00039680
rosa_samantha Rh5AG275100 Rh5DG288200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 111, 434
AccI GTMKAC 1 cut(s) 621
AccII CGCG 1 cut(s) 793
AciI CCGC 3 cut(s) 64, 615, 793
AclWI GGATC 4 cut(s) 49, 437, 653, 945
AcoI YGGCCR 1 cut(s) 829
AcsI RAATTY 1 cut(s) 4
AcuI CTGAAG 1 cut(s) 302
AfaI GTAC 4 cut(s) 202, 929, 1072, 1227
AfiI CCNNNNNNNGG 4 cut(s) 111, 412, 434, 1030
AgsI TTSAA 1 cut(s) 94
AjnI CCWGG 1 cut(s) 1066
AjuI GAANNNNNNNTTGG 2 cut(s) 399, 431
AluBI AGCT 5 cut(s) 74, 356, 554, 562, 1077
AluI AGCT 5 cut(s) 74, 356, 554, 562, 1077
Alw26I GTCTC 7 cut(s) 106, 338, 539, 563, 645, 958, 1137
AlwI GGATC 4 cut(s) 49, 437, 653, 945
AlwNI CAGNNNCTG 1 cut(s) 23
Ama87I CYCGRG 1 cut(s) 549
AoxI GGCC 1 cut(s) 829
ApeKI GCWGC 2 cut(s) 67, 356
ApoI RAATTY 1 cut(s) 4
AspLEI GCGC 1 cut(s) 635
AsuHPI GGTGA 2 cut(s) 137, 679
AsuII TTCGAA 1 cut(s) 229
AsuNHI GCTAGC 1 cut(s) 194
AvaI CYCGRG 1 cut(s) 549
BalI TGGCCA 1 cut(s) 831
BarI GAAGNNNNNNTAC 2 cut(s) 30, 62
BauI CACGAG 1 cut(s) 1134
BbsI GAAGAC 1 cut(s) 594
BbvI GCAGC 2 cut(s) 54, 343
BccI CCATC 1 cut(s) 1181
BceAI ACGGC 2 cut(s) 183, 725
BciT130I CCWGG 1 cut(s) 1068
BcoDI GTCTC 7 cut(s) 106, 338, 539, 563, 645, 958, 1137
BfaI CTAG 3 cut(s) 195, 747, 1074
BfmI CTRYAG 2 cut(s) 24, 357
BisI GCNGC 3 cut(s) 65, 68, 357
BlsI GCNGC 3 cut(s) 66, 69, 358
Bme1390I CCNGG 1 cut(s) 1068
BmeT110I CYCGRG 1 cut(s) 549
BmrFI CCNGG 1 cut(s) 1068
BmrI ACTGGG 1 cut(s) 601
BmtI GCTAGC 1 cut(s) 198
BmuI ACTGGG 1 cut(s) 601
BpiI GAAGAC 1 cut(s) 594
BpmI CTGGAG 1 cut(s) 587
Bpu14I TTCGAA 1 cut(s) 229
BsaBI GATNNNNATC 2 cut(s) 942, 1011
BsaI GGTCTC 2 cut(s) 338, 645
BsaWI WCCGGW 1 cut(s) 182
BsaXI ACNNNNNCTCC 2 cut(s) 603, 633
Bsc4I CCNNNNNNNGG 4 cut(s) 111, 412, 434, 1030
Bse1I ACTGG 2 cut(s) 116, 596
Bse3DI GCAATG 2 cut(s) 334, 634
Bse8I GATNNNNATC 2 cut(s) 942, 1011
BseBI CCWGG 1 cut(s) 1068
BseGI GGATG 3 cut(s) 219, 373, 1084
BseJI GATNNNNATC 2 cut(s) 942, 1011
BseLI CCNNNNNNNGG 4 cut(s) 111, 412, 434, 1030
BseMI GCAATG 2 cut(s) 334, 634
BseMII CTCAG 2 cut(s) 534, 1106
BseNI ACTGG 2 cut(s) 116, 596
BseRI GAGGAG 5 cut(s) 744, 913, 1046, 1049, 1080
BseXI GCAGC 2 cut(s) 54, 343
Bsh1236I CGCG 1 cut(s) 793
BshFI GGCC 1 cut(s) 831
BsiHKCI CYCGRG 1 cut(s) 549
BsiSI CCGG 1 cut(s) 183
BslFI GGGAC 3 cut(s) 256, 917, 1215
BslI CCNNNNNNNGG 4 cut(s) 111, 412, 434, 1030
BsmAI GTCTC 7 cut(s) 106, 338, 539, 563, 645, 958, 1137
BsmBI CGTCTC 1 cut(s) 106
BsmFI GGGAC 3 cut(s) 256, 917, 1215
BsmI GAATGC 1 cut(s) 1151
BsnI GGCC 1 cut(s) 831
Bso31I GGTCTC 2 cut(s) 338, 645
BsoBI CYCGRG 1 cut(s) 549
Bsp119I TTCGAA 1 cut(s) 229
Bsp143I GATC 7 cut(s) 54, 442, 645, 802, 937, 1012, 1142
BspACI CCGC 3 cut(s) 64, 615, 793
BspANI GGCC 1 cut(s) 831
BspCNI CTCAG 2 cut(s) 535, 1105
BspFNI CGCG 1 cut(s) 793
BspMAI CTGCAG 1 cut(s) 361
BspOI GCTAGC 1 cut(s) 198
BspPI GGATC 4 cut(s) 49, 437, 653, 945
BspT104I TTCGAA 1 cut(s) 229
BspTNI GGTCTC 2 cut(s) 338, 645
BsrDI GCAATG 2 cut(s) 334, 634
BsrI ACTGG 2 cut(s) 116, 596
BssMI GATC 7 cut(s) 54, 442, 645, 802, 937, 1012, 1142
BssNAI GTATAC 1 cut(s) 622
BssSI CACGAG 1 cut(s) 1134
Bst1107I GTATAC 1 cut(s) 622
Bst2BI CACGAG 1 cut(s) 1134
Bst2UI CCWGG 1 cut(s) 1068
Bst4CI ACNGT 2 cut(s) 25, 663
Bst6I CTCTTC 2 cut(s) 231, 1056
BstBI TTCGAA 1 cut(s) 229
BstC8I GCNNGC 2 cut(s) 72, 196
BstDEI CTNAG 2 cut(s) 543, 1092
BstF5I GGATG 3 cut(s) 219, 373, 1084
BstFNI CGCG 1 cut(s) 793
BstHHI GCGC 1 cut(s) 635
BstKTI GATC 7 cut(s) 57, 445, 648, 805, 940, 1015, 1145
BstMAI GTCTC 7 cut(s) 106, 338, 539, 563, 645, 958, 1137
BstMBI GATC 7 cut(s) 54, 442, 645, 802, 937, 1012, 1142
BstNI CCWGG 1 cut(s) 1068
BstNSI RCATGY 1 cut(s) 1064
BstSCI CCNGG 1 cut(s) 1066
BstSFI CTRYAG 2 cut(s) 24, 357
BstUI CGCG 1 cut(s) 793
BstV1I GCAGC 2 cut(s) 54, 343
BstV2I GAAGAC 1 cut(s) 594
BstX2I RGATCY 3 cut(s) 54, 442, 937
BstYI RGATCY 3 cut(s) 54, 442, 937
BstZ17I GTATAC 1 cut(s) 622
BsuRI GGCC 1 cut(s) 831
BtsCI GGATG 3 cut(s) 219, 373, 1084
BtsIMutI CAGTG 1 cut(s) 109
Cac8I GCNNGC 2 cut(s) 72, 196
CaiI CAGNNNCTG 1 cut(s) 23
CfoI GCGC 1 cut(s) 635
CseI GACGC 1 cut(s) 88
Csp6I GTAC 4 cut(s) 201, 928, 1071, 1226
CviAII CATG 6 cut(s) 281, 932, 963, 1061, 1175, 1184
CviQI GTAC 4 cut(s) 201, 928, 1071, 1226
DdeI CTNAG 2 cut(s) 543, 1092
DpnI GATC 7 cut(s) 56, 444, 647, 804, 939, 1014, 1144
DpnII GATC 7 cut(s) 54, 442, 645, 802, 937, 1012, 1142
EaeI YGGCCR 1 cut(s) 829
Eam1104I CTCTTC 2 cut(s) 231, 1056
EarI CTCTTC 2 cut(s) 231, 1056
EciI GGCGGA 1 cut(s) 630
Eco31I GGTCTC 2 cut(s) 338, 645
Eco57I CTGAAG 1 cut(s) 302
Eco88I CYCGRG 1 cut(s) 549
EcoRI GAATTC 1 cut(s) 4
EcoRII CCWGG 1 cut(s) 1066
Esp3I CGTCTC 1 cut(s) 106
FaeI CATG 6 cut(s) 284, 935, 966, 1064, 1178, 1187
FaqI GGGAC 3 cut(s) 256, 917, 1215
FatI CATG 6 cut(s) 280, 931, 962, 1060, 1174, 1183
FblI GTMKAC 1 cut(s) 621
Fnu4HI GCNGC 3 cut(s) 65, 68, 357
FokI GGATG 3 cut(s) 206, 360, 1071
Fsp4HI GCNGC 3 cut(s) 65, 68, 357
FspBI CTAG 3 cut(s) 195, 747, 1074
GlaI GCGC 1 cut(s) 634
GluI GCNGC 3 cut(s) 65, 68, 357
GsuI CTGGAG 1 cut(s) 587
HaeIII GGCC 1 cut(s) 831
HapII CCGG 1 cut(s) 183
HgaI GACGC 1 cut(s) 88
HhaI GCGC 1 cut(s) 635
Hin1II CATG 6 cut(s) 284, 935, 966, 1064, 1178, 1187
Hin6I GCGC 1 cut(s) 633
HinP1I GCGC 1 cut(s) 633
HindIII AAGCTT 1 cut(s) 560
HinfI GANTC 5 cut(s) 296, 547, 796, 821, 1130
HpaII CCGG 1 cut(s) 183
HphI GGTGA 2 cut(s) 137, 679
Hpy166II GTNNAC 1 cut(s) 622
Hpy188I TCNGA 4 cut(s) 544, 581, 650, 781
Hpy188III TCNNGA 3 cut(s) 566, 800, 1159
Hpy8I GTNNAC 1 cut(s) 622
HpyAV CCTTC 2 cut(s) 489, 883
HpyCH4III ACNGT 2 cut(s) 25, 663
HpyCH4V TGCA 6 cut(s) 327, 359, 627, 703, 1060, 1151
HpyF3I CTNAG 2 cut(s) 543, 1092
Hsp92II CATG 6 cut(s) 284, 935, 966, 1064, 1178, 1187
HspAI GCGC 1 cut(s) 633
Kzo9I GATC 7 cut(s) 54, 442, 645, 802, 937, 1012, 1142
LmnI GCTCC 2 cut(s) 215, 775
Lsp1109I GCAGC 2 cut(s) 54, 343
MaeI CTAG 3 cut(s) 195, 747, 1074
MaeIII GTNAC 2 cut(s) 143, 763
MalI GATC 7 cut(s) 56, 444, 647, 804, 939, 1014, 1144
MboI GATC 7 cut(s) 54, 442, 645, 802, 937, 1012, 1142
MflI RGATCY 3 cut(s) 54, 442, 937
MlsI TGGCCA 1 cut(s) 831
MluCI AATT 5 cut(s) 4, 137, 407, 878, 1114
MluNI TGGCCA 1 cut(s) 831
MlyI GAGTC 3 cut(s) 541, 830, 1139
Mox20I TGGCCA 1 cut(s) 831
MscI TGGCCA 1 cut(s) 831
MseI TTAA 5 cut(s) 258, 267, 513, 942, 1234
MslI CAYNNNNRTG 3 cut(s) 708, 961, 1026
Msp20I TGGCCA 1 cut(s) 831
MspI CCGG 1 cut(s) 183
MspR9I CCNGG 1 cut(s) 1068
Mva1269I GAATGC 1 cut(s) 1151
MvaI CCWGG 1 cut(s) 1068
MvnI CGCG 1 cut(s) 793
NdeII GATC 7 cut(s) 54, 442, 645, 802, 937, 1012, 1142
NheI GCTAGC 1 cut(s) 194
NlaIII CATG 6 cut(s) 284, 935, 966, 1064, 1178, 1187
NmuCI GTSAC 1 cut(s) 143
NspI RCATGY 1 cut(s) 1064
NspV TTCGAA 1 cut(s) 229
PaeR7I CTCGAG 1 cut(s) 549
PctI GAATGC 1 cut(s) 1151
PfeI GAWTC 2 cut(s) 296, 796
PflFI GACNNNGTC 1 cut(s) 593
PflMI CCANNNNNTGG 2 cut(s) 111, 434
PkrI GCNGC 3 cut(s) 66, 69, 358
PleI GAGTC 3 cut(s) 541, 829, 1138
PpsI GAGTC 3 cut(s) 541, 829, 1138
Psp6I CCWGG 1 cut(s) 1066
PspGI CCWGG 1 cut(s) 1066
PspXI VCTCGAGB 1 cut(s) 549
PstI CTGCAG 1 cut(s) 361
PstNI CAGNNNCTG 1 cut(s) 23
PsuI RGATCY 3 cut(s) 54, 442, 937
PsyI GACNNNGTC 1 cut(s) 593
RsaI GTAC 4 cut(s) 202, 929, 1072, 1227
RsaNI GTAC 4 cut(s) 201, 928, 1071, 1226
RseI CAYNNNNRTG 3 cut(s) 708, 961, 1026
SaqAI TTAA 5 cut(s) 258, 267, 513, 942, 1234
SatI GCNGC 3 cut(s) 65, 68, 357
Sau3AI GATC 7 cut(s) 54, 442, 645, 802, 937, 1012, 1142
SchI GAGTC 3 cut(s) 541, 830, 1139
ScrFI CCNGG 1 cut(s) 1068
SfcI CTRYAG 2 cut(s) 24, 357
Sfr274I CTCGAG 1 cut(s) 549
SfuI TTCGAA 1 cut(s) 229
SlaI CTCGAG 1 cut(s) 549
SmiMI CAYNNNNRTG 3 cut(s) 708, 961, 1026
SmlI CTYRAG 1 cut(s) 549
SmoI CTYRAG 1 cut(s) 549
Sse9I AATT 5 cut(s) 4, 137, 407, 878, 1114
SsiI CCGC 3 cut(s) 64, 615, 793
SspMI CTAG 3 cut(s) 195, 747, 1074
StyD4I CCNGG 1 cut(s) 1066
TaaI ACNGT 2 cut(s) 25, 663
TaqI TCGA 5 cut(s) 36, 229, 550, 1141, 1222
TasI AATT 5 cut(s) 4, 137, 407, 878, 1114
TauI GCSGC 1 cut(s) 67
TfiI GAWTC 2 cut(s) 296, 796
Tru1I TTAA 5 cut(s) 258, 267, 513, 942, 1234
Tru9I TTAA 5 cut(s) 258, 267, 513, 942, 1234
TscAI CASTG 1 cut(s) 116
TseFI GTSAC 1 cut(s) 143
TseI GCWGC 2 cut(s) 67, 356
Tsp45I GTSAC 1 cut(s) 143
TspDTI ATGAA 6 cut(s) 17, 208, 528, 629, 1023, 1073
TspGWI ACGGA 2 cut(s) 762, 936
TspRI CASTG 1 cut(s) 116
Tth111I GACNNNGTC 1 cut(s) 593
Van91I CCANNNNNTGG 2 cut(s) 111, 434
XapI RAATTY 1 cut(s) 4
XceI RCATGY 1 cut(s) 1064
XhoI CTCGAG 1 cut(s) 549
XmiI GTMKAC 1 cut(s) 621
XspI CTAG 3 cut(s) 195, 747, 1074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.