Rh5DG288200

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0019934
Physical Location & Seq
Reverse (-)
35858747 .. 35864715
5969 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG288200.1

Sequence Viewer

Length: 951 bp
ATGGAGAGGAAGAGCGTCCACCACAAGATGAATTCATCATCAGCAGAAACCGTAGCCAACATCGAAGAACTGCTACAACAGATCCTTGTGCGGCTGCCAGCTCTATCTGTGCTTCGTTTCAAGTGCGTCTCCAAGCACTGGCTCTCTCTTATCTCAACCCCCCAATTCTGTCACCTTCACACCCTCCAAAACCCAAACCCTAAAATCTCCGGTTTCTTCGCTAGCCGTACCTACTGCTCCACTTTCATCCCTCTTCAAAACCAATCTGGGACGACTAATCCTTTTAACGCTCTTAATGATTTCGTCCATGCTGACGACCCAGATACGTTAGGGTTAGATATTCTTCAGTCCTGCAATGGTCTCTTTCTCTGCCGCCATAGAGTTGGTCTCTTTCTCTGCCACCATAGAGTTGCAGACTTGAGAAAACGTCATCCCGTATATGTTGTCAATCCCACAACCAACCAATTCTCGGTTCTTTCCTCCCCAAGAGTTGGGAAAGATCCATTCTTTATTCTCTATGCTTTGGCTTTTGACCCTTCCAAATCGCCTCATTACAAGGTGGTCTGCCTTAATGACCACCAAATAGACATATATTCATCTGAGACTCGAGCTTGGAAGCTTCTGGAGACTTCTTTCAAAACAGAAGACTGGGGCAGTAGTAGGGAAGGCGGAGTATACTGCAATGGCGCAGTTCATTGGATAAGAGACCACACAAAGCTGGTGGATAGGGAGATATCATCTCATACTACAAAATATGGAAAGAACTGCTCCAGGAAATATAGACTAAGGGAGGAAACAAAGATAAAGGAAAAACAAAAAAAAGAAGGATGTGAAATACAAATGGTGTCGATTATGGAGATCCAAACTGTTATAAGAAGGGCTCACCTTCAAGTGTCTCATTTTCAATTGGCCTATGTAAAGCAGGCTATGATGGATCGCAAGCACACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

316

Amino Acids

36.42

Weight (kDa)

9.31

Isoelectric Point (pI)

43.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 22 - 57 5.6e-07 F-box domain
FBA_1 PF07734 115 - 237 4.2e-10 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019934)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0041051
rosa_laevigata RLG00000034021
rosa_multiflora Rmu_co8329617.1_g000001 Rmu_sc0002531.1_g000065
rosa_roxburghii Rroxscaffold_1G00039680
rosa_samantha Rh5AG275100 Rh5DG288200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 872
AccB7I CCANNNNNTGG 2 cut(s) 138, 491
AccI GTMKAC 1 cut(s) 675
AciI CCGC 3 cut(s) 91, 373, 669
AclWI GGATC 4 cut(s) 76, 494, 853, 942
AcsI RAATTY 1 cut(s) 31
AcuI CTGAAG 1 cut(s) 329
AfaI GTAC 1 cut(s) 229
AfiI CCNNNNNNNGG 3 cut(s) 138, 469, 491
AgsI TTSAA 5 cut(s) 121, 257, 637, 890, 905
AjnI CCWGG 1 cut(s) 770
AjuI GAANNNNNNNTTGG 2 cut(s) 456, 488
AluBI AGCT 4 cut(s) 101, 611, 619, 718
AluI AGCT 4 cut(s) 101, 611, 619, 718
Alw26I GTCTC 7 cut(s) 133, 365, 392, 596, 620, 699, 900
AlwI GGATC 4 cut(s) 76, 494, 853, 942
Ama87I CYCGRG 1 cut(s) 606
AoxI GGCC 1 cut(s) 909
ApeKI GCWGC 1 cut(s) 94
ApoI RAATTY 1 cut(s) 31
AspLEI GCGC 1 cut(s) 689
AsuHPI GGTGA 2 cut(s) 164, 875
AsuNHI GCTAGC 1 cut(s) 221
AvaI CYCGRG 1 cut(s) 606
BanII GRGCYC 1 cut(s) 883
BarI GAAGNNNNNNTAC 2 cut(s) 57, 89
BbsI GAAGAC 1 cut(s) 651
BbvI GCAGC 1 cut(s) 81
BccI CCATC 1 cut(s) 925
BceAI ACGGC 1 cut(s) 210
BciT130I CCWGG 1 cut(s) 772
BcoDI GTCTC 7 cut(s) 133, 365, 392, 596, 620, 699, 900
BfaI CTAG 1 cut(s) 222
BisI GCNGC 3 cut(s) 92, 95, 373
BlsI GCNGC 3 cut(s) 93, 96, 374
Bme1390I CCNGG 1 cut(s) 772
BmeT110I CYCGRG 1 cut(s) 606
BmrFI CCNGG 1 cut(s) 772
BmrI ACTGGG 1 cut(s) 658
BmtI GCTAGC 1 cut(s) 225
BmuI ACTGGG 1 cut(s) 658
BpiI GAAGAC 1 cut(s) 651
BplI GAGNNNNNCTC 2 cut(s) 372, 404
BpmI CTGGAG 2 cut(s) 644, 754
BpuEI CTTGAG 1 cut(s) 439
BsaI GGTCTC 3 cut(s) 365, 392, 699
BsaWI WCCGGW 1 cut(s) 209
Bsc4I CCNNNNNNNGG 3 cut(s) 138, 469, 491
Bse1I ACTGG 2 cut(s) 143, 653
Bse3DI GCAATG 2 cut(s) 361, 688
BseBI CCWGG 1 cut(s) 772
BseGI GGATG 3 cut(s) 246, 430, 833
BseLI CCNNNNNNNGG 3 cut(s) 138, 469, 491
BseMI GCAATG 2 cut(s) 361, 688
BseMII CTCAG 1 cut(s) 591
BseNI ACTGG 2 cut(s) 143, 653
BseXI GCAGC 1 cut(s) 81
BshFI GGCC 1 cut(s) 911
BsiHKCI CYCGRG 1 cut(s) 606
BsiSI CCGG 1 cut(s) 210
BslFI GGGAC 1 cut(s) 283
BslI CCNNNNNNNGG 3 cut(s) 138, 469, 491
BsmAI GTCTC 7 cut(s) 133, 365, 392, 596, 620, 699, 900
BsmBI CGTCTC 1 cut(s) 133
BsmFI GGGAC 1 cut(s) 283
BsnI GGCC 1 cut(s) 911
Bso31I GGTCTC 3 cut(s) 365, 392, 699
BsoBI CYCGRG 1 cut(s) 606
Bsp1286I GDGCHC 1 cut(s) 883
Bsp143I GATC 4 cut(s) 81, 499, 858, 934
BspACI CCGC 3 cut(s) 91, 373, 669
BspANI GGCC 1 cut(s) 911
BspCNI CTCAG 1 cut(s) 592
BspOI GCTAGC 1 cut(s) 225
BspPI GGATC 4 cut(s) 76, 494, 853, 942
BspQI GCTCTTC 1 cut(s) 5
BspTNI GGTCTC 3 cut(s) 365, 392, 699
BsrDI GCAATG 2 cut(s) 361, 688
BsrI ACTGG 2 cut(s) 143, 653
BssMI GATC 4 cut(s) 81, 499, 858, 934
BssNAI GTATAC 1 cut(s) 676
Bst1107I GTATAC 1 cut(s) 676
Bst2UI CCWGG 1 cut(s) 772
Bst4CI ACNGT 2 cut(s) 52, 868
Bst6I CTCTTC 2 cut(s) 5, 258
BstC8I GCNNGC 4 cut(s) 99, 223, 924, 941
BstDEI CTNAG 2 cut(s) 600, 785
BstF5I GGATG 3 cut(s) 246, 430, 833
BstHHI GCGC 1 cut(s) 689
BstKTI GATC 4 cut(s) 84, 502, 861, 937
BstMAI GTCTC 7 cut(s) 133, 365, 392, 596, 620, 699, 900
BstMBI GATC 4 cut(s) 81, 499, 858, 934
BstNI CCWGG 1 cut(s) 772
BstSCI CCNGG 1 cut(s) 770
BstV1I GCAGC 1 cut(s) 81
BstV2I GAAGAC 1 cut(s) 651
BstX2I RGATCY 3 cut(s) 81, 499, 858
BstXI CCANNNNNNTGG 1 cut(s) 383
BstYI RGATCY 3 cut(s) 81, 499, 858
BstZ17I GTATAC 1 cut(s) 676
BsuRI GGCC 1 cut(s) 911
BtsCI GGATG 3 cut(s) 246, 430, 833
BtsIMutI CAGTG 1 cut(s) 136
Cac8I GCNNGC 4 cut(s) 99, 223, 924, 941
CfoI GCGC 1 cut(s) 689
CseI GACGC 2 cut(s) 4, 115
Csp6I GTAC 1 cut(s) 228
CspCI CAANNNNNGTGG 2 cut(s) 702, 737
CviAII CATG 1 cut(s) 308
CviQI GTAC 1 cut(s) 228
DdeI CTNAG 2 cut(s) 600, 785
DpnI GATC 4 cut(s) 83, 501, 860, 936
DpnII GATC 4 cut(s) 81, 499, 858, 934
Eam1104I CTCTTC 2 cut(s) 5, 258
EarI CTCTTC 2 cut(s) 5, 258
EciI GGCGGA 1 cut(s) 684
Eco24I GRGCYC 1 cut(s) 883
Eco31I GGTCTC 3 cut(s) 365, 392, 699
Eco32I GATATC 1 cut(s) 735
Eco57I CTGAAG 1 cut(s) 329
Eco88I CYCGRG 1 cut(s) 606
EcoRI GAATTC 1 cut(s) 31
EcoRII CCWGG 1 cut(s) 770
EcoRV GATATC 1 cut(s) 735
EcoT38I GRGCYC 1 cut(s) 883
Esp3I CGTCTC 1 cut(s) 133
FaeI CATG 1 cut(s) 311
FaqI GGGAC 1 cut(s) 283
FatI CATG 1 cut(s) 307
FblI GTMKAC 1 cut(s) 675
Fnu4HI GCNGC 3 cut(s) 92, 95, 373
FokI GGATG 3 cut(s) 233, 417, 840
FriOI GRGCYC 1 cut(s) 883
Fsp4HI GCNGC 3 cut(s) 92, 95, 373
FspBI CTAG 1 cut(s) 222
GlaI GCGC 1 cut(s) 688
GluI GCNGC 3 cut(s) 92, 95, 373
GsuI CTGGAG 2 cut(s) 644, 754
HaeIII GGCC 1 cut(s) 911
HapII CCGG 1 cut(s) 210
HgaI GACGC 2 cut(s) 4, 115
HhaI GCGC 1 cut(s) 689
Hin1II CATG 1 cut(s) 311
Hin6I GCGC 1 cut(s) 687
HinP1I GCGC 1 cut(s) 687
HindIII AAGCTT 1 cut(s) 617
HinfI GANTC 1 cut(s) 604
HpaII CCGG 1 cut(s) 210
HphI GGTGA 2 cut(s) 164, 875
Hpy166II GTNNAC 2 cut(s) 19, 676
Hpy188I TCNGA 1 cut(s) 601
Hpy188III TCNNGA 1 cut(s) 623
Hpy8I GTNNAC 2 cut(s) 19, 676
HpyAV CCTTC 6 cut(s) 185, 546, 659, 818, 870, 896
HpyCH4III ACNGT 2 cut(s) 52, 868
HpyCH4IV ACGT 2 cut(s) 326, 427
HpyCH4V TGCA 3 cut(s) 354, 413, 681
HpyF3I CTNAG 2 cut(s) 600, 785
HpySE526I ACGT 2 cut(s) 326, 427
Hsp92II CATG 1 cut(s) 311
HspAI GCGC 1 cut(s) 687
Kzo9I GATC 4 cut(s) 81, 499, 858, 934
LguI GCTCTTC 1 cut(s) 5
LmnI GCTCC 2 cut(s) 242, 773
Lsp1109I GCAGC 1 cut(s) 81
MaeI CTAG 1 cut(s) 222
MaeII ACGT 2 cut(s) 326, 427
MaeIII GTNAC 1 cut(s) 170
MalI GATC 4 cut(s) 83, 501, 860, 936
MboI GATC 4 cut(s) 81, 499, 858, 934
MboII GAAGA 6 cut(s) 22, 77, 208, 245, 335, 656
MfeI CAATTG 1 cut(s) 905
MflI RGATCY 3 cut(s) 81, 499, 858
MhlI GDGCHC 1 cut(s) 883
MluCI AATT 4 cut(s) 31, 164, 464, 905
MlyI GAGTC 1 cut(s) 598
MnlI CCTC 5 cut(s) 194, 261, 490, 558, 784
MseI TTAA 3 cut(s) 285, 294, 570
MspI CCGG 1 cut(s) 210
MspR9I CCNGG 1 cut(s) 772
MunI CAATTG 1 cut(s) 905
MvaI CCWGG 1 cut(s) 772
NdeII GATC 4 cut(s) 81, 499, 858, 934
NheI GCTAGC 1 cut(s) 221
NlaIII CATG 1 cut(s) 311
NmuCI GTSAC 1 cut(s) 170
PaeR7I CTCGAG 1 cut(s) 606
PciSI GCTCTTC 1 cut(s) 5
PflMI CCANNNNNTGG 2 cut(s) 138, 491
PfoI TCCNGGA 1 cut(s) 770
PkrI GCNGC 3 cut(s) 93, 96, 374
PleI GAGTC 1 cut(s) 598
PpsI GAGTC 1 cut(s) 598
PsiI TTATAA 1 cut(s) 872
Psp6I CCWGG 1 cut(s) 770
PspGI CCWGG 1 cut(s) 770
PspXI VCTCGAGB 1 cut(s) 606
PsuI RGATCY 3 cut(s) 81, 499, 858
RsaI GTAC 1 cut(s) 229
RsaNI GTAC 1 cut(s) 228
SapI GCTCTTC 1 cut(s) 5
SaqAI TTAA 3 cut(s) 285, 294, 570
SatI GCNGC 3 cut(s) 92, 95, 373
Sau3AI GATC 4 cut(s) 81, 499, 858, 934
SchI GAGTC 1 cut(s) 598
ScrFI CCNGG 1 cut(s) 772
SduI GDGCHC 1 cut(s) 883
Sfr274I CTCGAG 1 cut(s) 606
SlaI CTCGAG 1 cut(s) 606
SmlI CTYRAG 2 cut(s) 418, 606
SmoI CTYRAG 2 cut(s) 418, 606
Sse9I AATT 4 cut(s) 31, 164, 464, 905
SsiI CCGC 3 cut(s) 91, 373, 669
SspMI CTAG 1 cut(s) 222
StyD4I CCNGG 1 cut(s) 770
TaaI ACNGT 2 cut(s) 52, 868
TaiI ACGT 2 cut(s) 329, 430
TaqI TCGA 3 cut(s) 63, 607, 848
TasI AATT 4 cut(s) 31, 164, 464, 905
TauI GCSGC 2 cut(s) 94, 375
Tru1I TTAA 3 cut(s) 285, 294, 570
Tru9I TTAA 3 cut(s) 285, 294, 570
TscAI CASTG 1 cut(s) 143
TseFI GTSAC 1 cut(s) 170
TseI GCWGC 1 cut(s) 94
Tsp45I GTSAC 1 cut(s) 170
TspDTI ATGAA 5 cut(s) 24, 44, 235, 585, 683
TspRI CASTG 1 cut(s) 143
Van91I CCANNNNNTGG 2 cut(s) 138, 491
XapI RAATTY 1 cut(s) 31
XhoI CTCGAG 1 cut(s) 606
XmiI GTMKAC 1 cut(s) 675
XspI CTAG 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.