Rh5AG275100

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0019934
Physical Location & Seq
Reverse (-)
36879327 .. 36884662
5336 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG275100.1

Sequence Viewer

Length: 936 bp
ATGAATTCATCATCAGCAGAAACCGTAGCCAACATCGAAGAACTGCTACAACAGATCCTTGTGCGGCTTCCAGCTCTATCTGTGCTTCGTTTCAAGTGCGTCTCCAAGCACTGGCTCTCTCTTATCTCAACCCCCCAATTCTGTCACCTTCACACCCTCCAAAACCCAAACCCTAAAATCTCCGGTTTCTTCGCTAGCAGTACCTACTGCTCCACTTTCATCCCTCTTCAAAACCAATCTGGGACGACTAATCCTTTTAACGCTCTTAATGATTTCGTCCATGCTGACGACCCAGATACGTTAGGGTTAGATATTCTTCAGTCCTGCAATGGTCTCTTTCTCTGCCGCCATAGAGTTGTTCCCTTTCTCTGCCACCATAGAGTTGCAGACTTGAGAAAACGTCATCCCGTATATGTTGTCAATCCCACAACCAACCAATTCTCGGTTCTTTCCTCCCCAAGAGTTGGGAAAGATCCATTCTATGCTTTGGCTTTTGACCCTTCCAAATCGCCTCATTACAAGGTGGTCTGCCTTGATGACCACCAAATAGACATATATTCATCTGAGACTCGAGCTTGGAAGCTTCTGGAGACTTCTTTCAAAACAGAAGACTGGGGCAGTAGTAGGGAAGGCGGAGTATACTGCAATGGCGCAGTTCATTGGATAAGAGACCACACACAGCTGGTGGATAGGTGGGTAAGAGATGATAGTGATGTGTTGCACTACTATGTTATTGGAGAAGATAGTTTGCGACTTGCCGTTTCTAGTCCTCCTCTGGTTGTAACTGCTCCGTATTCGGATTGCTTCTCGCGGATTCCAGATCATAGATATTTTGGGGAGTCTGGTCTTGGTTTGAAGAAGACTTGGCACAAGAGGGAACATAGATATCTTGGGAAGTCTAGTAGCCATGTGCACCTTTTTGATAATTTGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

311

Amino Acids

35.43

Weight (kDa)

7.29

Isoelectric Point (pI)

50.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 13 - 48 5.5e-07 F-box domain
FBA_1 PF07734 97 - 224 2.4e-10 F-box associated beta propeller domain
FBA_3 PF08268 101 - 224 9.4e-06 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019934)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0041051
rosa_laevigata RLG00000034021
rosa_multiflora Rmu_co8329617.1_g000001 Rmu_sc0002531.1_g000065
rosa_roxburghii Rroxscaffold_1G00039680
rosa_samantha Rh5AG275100 Rh5DG288200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 111, 464
AccI GTMKAC 1 cut(s) 639
AccII CGCG 1 cut(s) 811
AciI CCGC 4 cut(s) 64, 346, 633, 811
AclWI GGATC 2 cut(s) 49, 467
AcsI RAATTY 1 cut(s) 4
AcuI CTGAAG 1 cut(s) 302
AfaI GTAC 1 cut(s) 202
AfiI CCNNNNNNNGG 3 cut(s) 111, 442, 464
AgsI TTSAA 4 cut(s) 94, 230, 601, 856
AjuI GAANNNNNNNTTGG 2 cut(s) 429, 461
AluBI AGCT 4 cut(s) 74, 575, 583, 682
AluI AGCT 4 cut(s) 74, 575, 583, 682
Alw21I GWGCWC 1 cut(s) 915
Alw26I GTCTC 5 cut(s) 106, 338, 560, 584, 663
Alw44I GTGCAC 1 cut(s) 911
AlwI GGATC 2 cut(s) 49, 467
Ama87I CYCGRG 1 cut(s) 570
ApaLI GTGCAC 1 cut(s) 911
ApoI RAATTY 1 cut(s) 4
AspLEI GCGC 1 cut(s) 653
AsuHPI GGTGA 1 cut(s) 137
AsuNHI GCTAGC 1 cut(s) 194
AvaI CYCGRG 1 cut(s) 570
BaeGI GKGCMC 1 cut(s) 915
BarI GAAGNNNNNNTAC 2 cut(s) 30, 62
BbsI GAAGAC 2 cut(s) 615, 866
Bbv12I GWGCWC 1 cut(s) 915
BceAI ACGGC 1 cut(s) 743
BcoDI GTCTC 5 cut(s) 106, 338, 560, 584, 663
BfaI CTAG 3 cut(s) 195, 765, 900
BisI GCNGC 2 cut(s) 65, 346
BlsI GCNGC 2 cut(s) 66, 347
BmeT110I CYCGRG 1 cut(s) 570
BmrI ACTGGG 1 cut(s) 622
BmtI GCTAGC 1 cut(s) 198
BmuI ACTGGG 1 cut(s) 622
BpiI GAAGAC 2 cut(s) 615, 866
BpmI CTGGAG 1 cut(s) 608
BpuEI CTTGAG 1 cut(s) 412
BsaI GGTCTC 2 cut(s) 338, 663
BsaWI WCCGGW 1 cut(s) 182
Bsc4I CCNNNNNNNGG 3 cut(s) 111, 442, 464
Bse1I ACTGG 2 cut(s) 116, 617
Bse3DI GCAATG 2 cut(s) 334, 652
BseGI GGATG 2 cut(s) 219, 403
BseLI CCNNNNNNNGG 3 cut(s) 111, 442, 464
BseMI GCAATG 2 cut(s) 334, 652
BseMII CTCAG 1 cut(s) 555
BseNI ACTGG 2 cut(s) 116, 617
BseRI GAGGAG 1 cut(s) 762
BseSI GKGCMC 1 cut(s) 915
Bsh1236I CGCG 1 cut(s) 811
BsiHKAI GWGCWC 1 cut(s) 915
BsiHKCI CYCGRG 1 cut(s) 570
BsiSI CCGG 1 cut(s) 183
BslFI GGGAC 1 cut(s) 256
BslI CCNNNNNNNGG 3 cut(s) 111, 442, 464
BsmAI GTCTC 5 cut(s) 106, 338, 560, 584, 663
BsmBI CGTCTC 1 cut(s) 106
BsmFI GGGAC 1 cut(s) 256
Bso31I GGTCTC 2 cut(s) 338, 663
BsoBI CYCGRG 1 cut(s) 570
Bsp1286I GDGCHC 1 cut(s) 915
Bsp143I GATC 3 cut(s) 54, 472, 820
BspACI CCGC 4 cut(s) 64, 346, 633, 811
BspCNI CTCAG 1 cut(s) 556
BspFNI CGCG 1 cut(s) 811
BspOI GCTAGC 1 cut(s) 198
BspPI GGATC 2 cut(s) 49, 467
BspTNI GGTCTC 2 cut(s) 338, 663
BsrDI GCAATG 2 cut(s) 334, 652
BsrI ACTGG 2 cut(s) 116, 617
BssMI GATC 3 cut(s) 54, 472, 820
BssNAI GTATAC 1 cut(s) 640
Bst1107I GTATAC 1 cut(s) 640
Bst4CI ACNGT 1 cut(s) 25
Bst6I CTCTTC 1 cut(s) 231
BstC8I GCNNGC 1 cut(s) 196
BstDEI CTNAG 1 cut(s) 564
BstF5I GGATG 2 cut(s) 219, 403
BstFNI CGCG 1 cut(s) 811
BstHHI GCGC 1 cut(s) 653
BstKTI GATC 3 cut(s) 57, 475, 823
BstMAI GTCTC 5 cut(s) 106, 338, 560, 584, 663
BstMBI GATC 3 cut(s) 54, 472, 820
BstSLI GKGCMC 1 cut(s) 915
BstUI CGCG 1 cut(s) 811
BstV2I GAAGAC 2 cut(s) 615, 866
BstX2I RGATCY 2 cut(s) 54, 472
BstYI RGATCY 2 cut(s) 54, 472
BstZ17I GTATAC 1 cut(s) 640
BtsCI GGATG 2 cut(s) 219, 403
BtsIMutI CAGTG 1 cut(s) 109
Cac8I GCNNGC 1 cut(s) 196
CfoI GCGC 1 cut(s) 653
CseI GACGC 1 cut(s) 88
Csp6I GTAC 1 cut(s) 201
CviAII CATG 2 cut(s) 281, 908
CviJI RGCY 9 cut(s) 29, 67, 74, 115, 491, 575, 583, 682, 906
CviKI_1 RGCY 9 cut(s) 29, 67, 74, 115, 491, 575, 583, 682, 906
CviQI GTAC 1 cut(s) 201
DdeI CTNAG 1 cut(s) 564
DpnI GATC 3 cut(s) 56, 474, 822
DpnII GATC 3 cut(s) 54, 472, 820
Eam1104I CTCTTC 1 cut(s) 231
EarI CTCTTC 1 cut(s) 231
EciI GGCGGA 1 cut(s) 648
Eco31I GGTCTC 2 cut(s) 338, 663
Eco32I GATATC 1 cut(s) 887
Eco57I CTGAAG 1 cut(s) 302
Eco88I CYCGRG 1 cut(s) 570
EcoRI GAATTC 1 cut(s) 4
EcoRV GATATC 1 cut(s) 887
Esp3I CGTCTC 1 cut(s) 106
FaeI CATG 2 cut(s) 284, 911
FaqI GGGAC 1 cut(s) 256
FatI CATG 2 cut(s) 280, 907
FblI GTMKAC 1 cut(s) 639
Fnu4HI GCNGC 2 cut(s) 65, 346
FokI GGATG 2 cut(s) 206, 390
Fsp4HI GCNGC 2 cut(s) 65, 346
FspBI CTAG 3 cut(s) 195, 765, 900
GlaI GCGC 1 cut(s) 652
GluI GCNGC 2 cut(s) 65, 346
GsuI CTGGAG 1 cut(s) 608
HapII CCGG 1 cut(s) 183
HgaI GACGC 1 cut(s) 88
HhaI GCGC 1 cut(s) 653
Hin1II CATG 2 cut(s) 284, 911
Hin6I GCGC 1 cut(s) 651
HinP1I GCGC 1 cut(s) 651
HindIII AAGCTT 1 cut(s) 581
HinfI GANTC 3 cut(s) 568, 814, 839
HpaII CCGG 1 cut(s) 183
HphI GGTGA 1 cut(s) 137
Hpy166II GTNNAC 2 cut(s) 640, 913
Hpy188I TCNGA 2 cut(s) 565, 799
Hpy188III TCNNGA 2 cut(s) 587, 818
Hpy8I GTNNAC 2 cut(s) 640, 913
HpyAV CCTTC 3 cut(s) 158, 510, 623
HpyCH4III ACNGT 1 cut(s) 25
HpyCH4IV ACGT 2 cut(s) 299, 400
HpyCH4V TGCA 5 cut(s) 327, 386, 645, 721, 913
HpyF3I CTNAG 1 cut(s) 564
HpySE526I ACGT 2 cut(s) 299, 400
Hsp92II CATG 2 cut(s) 284, 911
HspAI GCGC 1 cut(s) 651
Kzo9I GATC 3 cut(s) 54, 472, 820
LmnI GCTCC 2 cut(s) 215, 793
MaeI CTAG 3 cut(s) 195, 765, 900
MaeII ACGT 2 cut(s) 299, 400
MaeIII GTNAC 2 cut(s) 143, 781
MalI GATC 3 cut(s) 56, 474, 822
MboI GATC 3 cut(s) 54, 472, 820
MboII GAAGA 8 cut(s) 50, 181, 218, 308, 620, 752, 868, 871
MflI RGATCY 2 cut(s) 54, 472
MhlI GDGCHC 1 cut(s) 915
MluCI AATT 4 cut(s) 4, 137, 437, 925
MlyI GAGTC 2 cut(s) 562, 848
MnlI CCTC 7 cut(s) 167, 234, 463, 522, 780, 783, 867
MseI TTAA 2 cut(s) 258, 267
MslI CAYNNNNRTG 1 cut(s) 726
MspA1I CMGCKG 1 cut(s) 682
MspI CCGG 1 cut(s) 183
MvnI CGCG 1 cut(s) 811
NdeII GATC 3 cut(s) 54, 472, 820
NheI GCTAGC 1 cut(s) 194
NlaIII CATG 2 cut(s) 284, 911
NmuCI GTSAC 1 cut(s) 143
PaeR7I CTCGAG 1 cut(s) 570
PfeI GAWTC 1 cut(s) 814
PflMI CCANNNNNTGG 2 cut(s) 111, 464
PkrI GCNGC 2 cut(s) 66, 347
PleI GAGTC 2 cut(s) 562, 847
PpsI GAGTC 2 cut(s) 562, 847
PspXI VCTCGAGB 1 cut(s) 570
PsuI RGATCY 2 cut(s) 54, 472
PvuII CAGCTG 1 cut(s) 682
RsaI GTAC 1 cut(s) 202
RsaNI GTAC 1 cut(s) 201
RseI CAYNNNNRTG 1 cut(s) 726
SaqAI TTAA 2 cut(s) 258, 267
SatI GCNGC 2 cut(s) 65, 346
Sau3AI GATC 3 cut(s) 54, 472, 820
SchI GAGTC 2 cut(s) 562, 848
SduI GDGCHC 1 cut(s) 915
Sfr274I CTCGAG 1 cut(s) 570
SlaI CTCGAG 1 cut(s) 570
SmiMI CAYNNNNRTG 1 cut(s) 726
SmlI CTYRAG 2 cut(s) 391, 570
SmoI CTYRAG 2 cut(s) 391, 570
Sse9I AATT 4 cut(s) 4, 137, 437, 925
SsiI CCGC 4 cut(s) 64, 346, 633, 811
SspMI CTAG 3 cut(s) 195, 765, 900
TaaI ACNGT 1 cut(s) 25
TaiI ACGT 2 cut(s) 302, 403
TaqI TCGA 2 cut(s) 36, 571
TasI AATT 4 cut(s) 4, 137, 437, 925
TauI GCSGC 2 cut(s) 67, 348
TfiI GAWTC 1 cut(s) 814
Tru1I TTAA 2 cut(s) 258, 267
Tru9I TTAA 2 cut(s) 258, 267
TscAI CASTG 1 cut(s) 116
TseFI GTSAC 1 cut(s) 143
Tsp45I GTSAC 1 cut(s) 143
TspDTI ATGAA 4 cut(s) 17, 208, 549, 647
TspGWI ACGGA 1 cut(s) 780
TspRI CASTG 1 cut(s) 116
Van91I CCANNNNNTGG 2 cut(s) 111, 464
VneI GTGCAC 1 cut(s) 911
XapI RAATTY 1 cut(s) 4
XhoI CTCGAG 1 cut(s) 570
XmiI GTMKAC 1 cut(s) 639
XspI CTAG 3 cut(s) 195, 765, 900
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.