Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0000050.1

Orthogroup ID

OG0012229

Physical Location & Seq

Genomic Coordinates Forward (+)

113673 .. 114848

Gene Structure 1176 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1176 bp

atgcctccgtcgcttgctgcctctgtcttcttcttcttcttctcagttcttggcctcatttcctcagtccactcgcacttatgctcaccgcaccatcaatctctctgcccaccctttacttcaactcctccttttcccttctctttctcacctggctgtggccacccctctttccaaatcaaatgctacgcaaatcactccataatcaccattaacaacctcgctttctccctcctccactacgatcccaactccacatctctccttctctccccctacatctccaactcgacaattaaagctaaatcccttagcactagcactagcactagcaactgctcatcccctcactttctttcaatccccagccgctccatcaacctctcctcttcccccttccgcttctccgacggttcctgcaaccgcctctctgttctcaaaccttgctccccgccaaatctccctaattgcagccattgtccctgggaatgcaagctcataaagagcccggtcaagctactccctgactgtggatctagtcgtagcgctgttcccaaccagggctgccaagccgatgtcttgggatatcttgacaacttcctccaactcgggatcgagctcgagtgggacgaagctcaagattcctacttgtcaagctgcagagattgcgaagccaacaacggcttctgcggcttcaattcctccgacccgtcacggcaattcctgtgtctccacaccaaagctcagctctcacccccatggatgagcaagcacaacccccatcgcgtcgccattttgtgctcggtcttggcagtgacttgtttgatggttctggtttcagtttctttggccataatcaggtccagaagaagactacactccgccgccacagaagaagacccgaccacattgttcctccagcgccaccgctccgccagtctcctcccgccggtgttcacgtacgaagagctggaatcatccaccaacaaattcgacaccaagcgcaaaatcggcgacggcgggttcgggtcagtctacctgggccagctctacgacagccgcgtcgtcgccgtaaagtacctccactccgccgcctccggcaaggccttctccaacaagtgcttctgcaacgaaatcctgatcctctcctccattgaccatcctaagagtcgataa

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

GUB_WAK_bind Pkinase Pkinase_Tyr WAK_assoc

Protein Analysis

391

Amino Acids

42.88

Weight (kDa)

8.55

Isoelectric Point (pI)

62.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WAK_assoc	PF14380	193 - 245	2.4e-06	Wall-associated receptor kinase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012229)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69910
fragaria_vesca	FvH4_4g35620
malus_domestica	MD13G1081800.v1.1 MD16G1080900.v1.1
prunus_persica	Prupe.1G265100_v2.0.a1
pyrus_communis	pycom13g07060 pycom16g06990
rosa_chinensis	RchiOBHm_Chr4g0445241
rosa_laevigata	RLG00000005756
rosa_multiflora	Rmu_co8464497.1_g000001 Rmu_sc0000050.1_g000028 Rmu_sc0000050.1_g000029
rosa_roxburghii	Rroxscaffold_5G00385740
rosa_rugosa	Rorug04G0360000
rosa_samantha	Rh4AG421100 Rh4BG433000 Rh4CG447600 Rh4DG429000
rosa_wichuraiana	Rw4G036110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1061
AccBSI	CCGCTC	2 cut(s)	372, 930
AccI	GTMKAC	1 cut(s)	1035
AccII	CGCG	2 cut(s)	786, 1062
AclWI	GGATC	4 cut(s)	239, 541, 620, 1135
AcoI	YGGCCR	2 cut(s)	160, 849
AcsI	RAATTY	1 cut(s)	989
AfaI	GTAC	2 cut(s)	962, 1079
AfeI	AGCGCT	1 cut(s)	547
AfiI	CCNNNNNNNGG	5 cut(s)	158, 530, 560, 858, 949
AgsI	TTSAA	3 cut(s)	123, 360, 697
AjnI	CCWGG	4 cut(s)	151, 482, 558, 1038
AjuI	GAANNNNNNNTTGG	4 cut(s)	668, 700, 1106, 1138
Alw21I	GWGCWC	2 cut(s)	621, 803
Alw26I	GTCTC	2 cut(s)	734, 944
AlwI	GGATC	4 cut(s)	239, 541, 620, 1135
Ama87I	CYCGRG	2 cut(s)	608, 620
Aor51HI	AGCGCT	1 cut(s)	547
AoxI	GGCC	5 cut(s)	52, 160, 849, 1042, 1104
ApeKI	GCWGC	4 cut(s)	17, 471, 564, 657
ApoI	RAATTY	1 cut(s)	989
ArsI	GACNNNNNNTTYG	2 cut(s)	1007, 1039
AspLEI	GCGC	3 cut(s)	548, 924, 1005
AspS9I	GGNCC	2 cut(s)	861, 1042
AsuC2I	CCSGG	1 cut(s)	509
AsuHPI	GGTGA	4 cut(s)	78, 141, 199, 744
AvaI	CYCGRG	2 cut(s)	608, 620
AvaII	GGWCC	1 cut(s)	861
BalI	TGGCCA	2 cut(s)	162, 851
BanII	GRGCYC	2 cut(s)	509, 621
BbsI	GAAGAC	3 cut(s)	19, 877, 903
Bbv12I	GWGCWC	2 cut(s)	621, 803
BbvI	GCAGC	4 cut(s)	4, 483, 551, 644
BccI	CCATC	5 cut(s)	102, 383, 789, 820, 1167
BceAI	ACGGC	4 cut(s)	697, 731, 1033, 1055
BciT130I	CCWGG	4 cut(s)	153, 484, 560, 1040
BcnI	CCSGG	1 cut(s)	509
BcoDI	GTCTC	2 cut(s)	734, 944
BfaI	CTAG	4 cut(s)	318, 324, 330, 537
BfmI	CTRYAG	1 cut(s)	658
BfoI	RGCGCY	2 cut(s)	549, 925
BisI	GCNGC	9 cut(s)	18, 370, 472, 565, 658, 691, 885, 1060, 1092
BlpI	GCTNAGC	1 cut(s)	744
BlsI	GCNGC	9 cut(s)	19, 371, 473, 566, 659, 692, 886, 1061, 1093
Bme1390I	CCNGG	5 cut(s)	153, 484, 509, 560, 1040
Bme18I	GGWCC	1 cut(s)	861
BmeT110I	CYCGRG	2 cut(s)	608, 620
BmgT120I	GGNCC	2 cut(s)	861, 1042
BmiI	GGNNCC	1 cut(s)	415
BmrFI	CCNGG	5 cut(s)	153, 484, 509, 560, 1040
BpiI	GAAGAC	3 cut(s)	19, 877, 903
BpmI	CTGGAG	1 cut(s)	902
Bpu10I	CCTNAGC	1 cut(s)	311
Bpu1102I	GCTNAGC	1 cut(s)	744
BpuEI	CTTGAG	1 cut(s)	621
BpuMI	CCSGG	1 cut(s)	509
BsaAI	YACGTR	1 cut(s)	960
BsaJI	CCNNGG	5 cut(s)	482, 483, 559, 758, 1039
BsaXI	ACNNNNNCTCC	4 cut(s)	109, 139, 1070, 1100
Bsc4I	CCNNNNNNNGG	5 cut(s)	158, 530, 560, 858, 949
Bse118I	RCCGGY	1 cut(s)	949
Bse1I	ACTGG	1 cut(s)	936
BseBI	CCWGG	4 cut(s)	153, 484, 560, 1040
BseDI	CCNNGG	5 cut(s)	482, 483, 559, 758, 1039
BseGI	GGATG	4 cut(s)	341, 768, 977, 1159
BseLI	CCNNNNNNNGG	5 cut(s)	158, 530, 560, 858, 949
BseMII	CTCAG	3 cut(s)	57, 78, 758
BseNI	ACTGG	1 cut(s)	936
BseRI	GAGGAG	5 cut(s)	117, 224, 376, 932, 1138
BseXI	GCAGC	4 cut(s)	4, 483, 551, 644
BseYI	CCCAGC	1 cut(s)	365
Bsh1236I	CGCG	2 cut(s)	786, 1062
BshFI	GGCC	5 cut(s)	54, 162, 851, 1044, 1106
BsiHKAI	GWGCWC	2 cut(s)	621, 803
BsiHKCI	CYCGRG	2 cut(s)	608, 620
BsiSI	CCGG	3 cut(s)	509, 950, 1098
BsiWI	CGTACG	1 cut(s)	960
BslFI	GGGAC	2 cut(s)	465, 641
BslI	CCNNNNNNNGG	5 cut(s)	158, 530, 560, 858, 949
BsmAI	GTCTC	2 cut(s)	734, 944
BsmFI	GGGAC	2 cut(s)	465, 641
BsmI	GAATGC	1 cut(s)	494
BsnI	GGCC	5 cut(s)	54, 162, 851, 1044, 1106
BsoBI	CYCGRG	2 cut(s)	608, 620
Bsp1286I	GDGCHC	3 cut(s)	509, 621, 803
Bsp143I	GATC	4 cut(s)	244, 533, 612, 1140
Bsp1720I	GCTNAGC	1 cut(s)	744
Bsp19I	CCATGG	1 cut(s)	758
BspANI	GGCC	5 cut(s)	54, 162, 851, 1044, 1106
BspCNI	CTCAG	3 cut(s)	56, 77, 757
BspFNI	CGCG	2 cut(s)	786, 1062
BspLI	GGNNCC	1 cut(s)	415
BspMAI	CTGCAG	1 cut(s)	662
BspPI	GGATC	4 cut(s)	239, 541, 620, 1135
BspQI	GCTCTTC	1 cut(s)	960
BsrBI	CCGCTC	2 cut(s)	372, 930
BsrFI	RCCGGY	1 cut(s)	949
BsrI	ACTGG	1 cut(s)	936
BssAI	RCCGGY	1 cut(s)	949
BssECI	CCNNGG	5 cut(s)	482, 483, 559, 758, 1039
BssMI	GATC	4 cut(s)	244, 533, 612, 1140
BssT1I	CCWWGG	1 cut(s)	758
Bst2UI	CCWGG	4 cut(s)	153, 484, 560, 1040
Bst4CI	ACNGT	2 cut(s)	413, 530
Bst6I	CTCTTC	2 cut(s)	394, 960
BstAPI	GCANNNNNTGC	1 cut(s)	666
BstBAI	YACGTR	1 cut(s)	960
BstC8I	GCNNGC	4 cut(s)	15, 494, 770, 1046
BstDEI	CTNAG	5 cut(s)	43, 64, 311, 744, 1164
BstDSI	CCRYGG	1 cut(s)	758
BstF5I	GGATG	4 cut(s)	341, 768, 977, 1159
BstFNI	CGCG	2 cut(s)	786, 1062
BstH2I	RGCGCY	2 cut(s)	549, 925
BstHHI	GCGC	3 cut(s)	548, 924, 1005
BstKTI	GATC	4 cut(s)	247, 536, 615, 1143
BstMAI	GTCTC	2 cut(s)	734, 944
BstMBI	GATC	4 cut(s)	244, 533, 612, 1140
BstMWI	GCNNNNNNNGC	4 cut(s)	10, 666, 690, 1011
BstNI	CCWGG	4 cut(s)	153, 484, 560, 1040
BstSCI	CCNGG	5 cut(s)	151, 482, 507, 558, 1038
BstSFI	CTRYAG	1 cut(s)	658
BstUI	CGCG	2 cut(s)	786, 1062
BstV1I	GCAGC	4 cut(s)	4, 483, 551, 644
BstV2I	GAAGAC	3 cut(s)	19, 877, 903
BstX2I	RGATCY	1 cut(s)	533
BstYI	RGATCY	1 cut(s)	533
BsuRI	GGCC	5 cut(s)	54, 162, 851, 1044, 1106
BtgI	CCRYGG	1 cut(s)	758
BtgZI	GCGATG	1 cut(s)	767
BtsCI	GGATG	4 cut(s)	341, 768, 977, 1159
BtsI	GCAGTG	1 cut(s)	819
BtsIMutI	CAGTG	1 cut(s)	819
Cac8I	GCNNGC	4 cut(s)	15, 494, 770, 1046
CfoI	GCGC	3 cut(s)	548, 924, 1005
Cfr10I	RCCGGY	1 cut(s)	949
Cfr13I	GGNCC	2 cut(s)	861, 1042
CseI	GACGC	2 cut(s)	775, 1051
Csp6I	GTAC	2 cut(s)	961, 1078
CviAII	CATG	1 cut(s)	759
CviQI	GTAC	2 cut(s)	961, 1078
DdeI	CTNAG	5 cut(s)	43, 64, 311, 744, 1164
DpnI	GATC	4 cut(s)	246, 535, 614, 1142
DpnII	GATC	4 cut(s)	244, 533, 612, 1140
DrdI	GACNNNNNNGTC	1 cut(s)	1061
DseDI	GACNNNNNNGTC	1 cut(s)	1061
EaeI	YGGCCR	2 cut(s)	160, 849
Eam1104I	CTCTTC	2 cut(s)	394, 960
EarI	CTCTTC	2 cut(s)	394, 960
EciI	GGCGGA	3 cut(s)	871, 922, 1078
Ecl136II	GAGCTC	1 cut(s)	619
Eco130I	CCWWGG	1 cut(s)	758
Eco147I	AGGCCT	1 cut(s)	1106
Eco24I	GRGCYC	2 cut(s)	509, 621
Eco32I	GATATC	1 cut(s)	587
Eco47I	GGWCC	1 cut(s)	861
Eco47III	AGCGCT	1 cut(s)	547
Eco53kI	GAGCTC	1 cut(s)	619
Eco88I	CYCGRG	2 cut(s)	608, 620
EcoICRI	GAGCTC	1 cut(s)	619
EcoRII	CCWGG	4 cut(s)	151, 482, 558, 1038
EcoRV	GATATC	1 cut(s)	587
EcoT14I	CCWWGG	1 cut(s)	758
EcoT38I	GRGCYC	2 cut(s)	509, 621
ErhI	CCWWGG	1 cut(s)	758
FaeI	CATG	1 cut(s)	762
FaiI	YATR	5 cut(s)	82, 203, 500, 760, 854
FaqI	GGGAC	2 cut(s)	465, 641
FatI	CATG	1 cut(s)	758
FauI	CCCGC	3 cut(s)	459, 954, 1013
FblI	GTMKAC	1 cut(s)	1035
Fnu4HI	GCNGC	9 cut(s)	18, 370, 472, 565, 658, 691, 885, 1060, 1092
FokI	GGATG	4 cut(s)	328, 775, 964, 1146
FriOI	GRGCYC	2 cut(s)	509, 621
Fsp4HI	GCNGC	9 cut(s)	18, 370, 472, 565, 658, 691, 885, 1060, 1092
FspBI	CTAG	4 cut(s)	318, 324, 330, 537
GlaI	GCGC	3 cut(s)	547, 923, 1004
GluI	GCNGC	9 cut(s)	18, 370, 472, 565, 658, 691, 885, 1060, 1092
GsaI	CCCAGC	1 cut(s)	369
GsuI	CTGGAG	1 cut(s)	902
HaeII	RGCGCY	2 cut(s)	549, 925
HaeIII	GGCC	5 cut(s)	54, 162, 851, 1044, 1106
HapII	CCGG	3 cut(s)	509, 950, 1098
HgaI	GACGC	2 cut(s)	775, 1051
HhaI	GCGC	3 cut(s)	548, 924, 1005
Hin1II	CATG	1 cut(s)	762
Hin6I	GCGC	3 cut(s)	546, 922, 1003
HinP1I	GCGC	3 cut(s)	546, 922, 1003
HinfI	GANTC	3 cut(s)	641, 974, 1168
HpaII	CCGG	3 cut(s)	509, 950, 1098
HphI	GGTGA	4 cut(s)	78, 141, 199, 744
Hpy166II	GTNNAC	3 cut(s)	70, 957, 1036
Hpy188I	TCNGA	2 cut(s)	409, 706
Hpy188III	TCNNGA	5 cut(s)	590, 610, 638, 864, 1138
Hpy8I	GTNNAC	3 cut(s)	70, 957, 1036
Hpy99I	CGWCG	6 cut(s)	13, 413, 791, 1019, 1067, 1070
HpyAV	CCTTC	4 cut(s)	148, 275, 406, 1117
HpyCH4III	ACNGT	2 cut(s)	413, 530
HpyCH4IV	ACGT	1 cut(s)	959
HpyCH4V	TGCA	5 cut(s)	420, 471, 492, 660, 1128
HpyF10VI	GCNNNNNNNGC	4 cut(s)	10, 666, 690, 1011
HpyF3I	CTNAG	5 cut(s)	43, 64, 311, 744, 1164
HpySE526I	ACGT	1 cut(s)	959
Hsp92II	CATG	1 cut(s)	762
HspAI	GCGC	3 cut(s)	546, 922, 1003
Kzo9I	GATC	4 cut(s)	244, 533, 612, 1140
LguI	GCTCTTC	1 cut(s)	960
LmnI	GCTCC	3 cut(s)	377, 452, 935
Lsp1109I	GCAGC	4 cut(s)	4, 483, 551, 644
MaeI	CTAG	4 cut(s)	318, 324, 330, 537
MaeII	ACGT	1 cut(s)	959
MaeIII	GTNAC	2 cut(s)	711, 814
MalI	GATC	4 cut(s)	246, 535, 614, 1142
MbiI	CCGCTC	2 cut(s)	372, 930
MboI	GATC	4 cut(s)	244, 533, 612, 1140
MflI	RGATCY	1 cut(s)	533
MhlI	GDGCHC	3 cut(s)	509, 621, 803
MlsI	TGGCCA	2 cut(s)	162, 851
MluCI	AATT	5 cut(s)	294, 466, 697, 719, 989
MluNI	TGGCCA	2 cut(s)	162, 851
MmeI	TCCRAC	5 cut(s)	309, 432, 628, 729, 1137
Mox20I	TGGCCA	2 cut(s)	162, 851
MscI	TGGCCA	2 cut(s)	162, 851
MseI	TTAA	2 cut(s)	213, 297
MslI	CAYNNNNRTG	1 cut(s)	757
Msp20I	TGGCCA	2 cut(s)	162, 851
MspI	CCGG	3 cut(s)	509, 950, 1098
MspR9I	CCNGG	5 cut(s)	153, 484, 509, 560, 1040
Mva1269I	GAATGC	1 cut(s)	494
MvaI	CCWGG	4 cut(s)	153, 484, 560, 1040
MvnI	CGCG	2 cut(s)	786, 1062
MwoI	GCNNNNNNNGC	4 cut(s)	10, 666, 690, 1011
NciI	CCSGG	1 cut(s)	509
NcoI	CCATGG	1 cut(s)	758
NdeII	GATC	4 cut(s)	244, 533, 612, 1140
NlaIII	CATG	1 cut(s)	762
NlaIV	GGNNCC	1 cut(s)	415
NmuCI	GTSAC	2 cut(s)	711, 814
PaeR7I	CTCGAG	1 cut(s)	620
PasI	CCCWGGG	1 cut(s)	483
PceI	AGGCCT	1 cut(s)	1106
PciSI	GCTCTTC	1 cut(s)	960
PcsI	WCGNNNNNNNCGW	2 cut(s)	627, 1059
PctI	GAATGC	1 cut(s)	494
PfeI	GAWTC	2 cut(s)	641, 974
Pfl23II	CGTACG	1 cut(s)	960
PkrI	GCNGC	9 cut(s)	19, 371, 473, 566, 659, 692, 886, 1061, 1093
Ppu21I	YACGTR	1 cut(s)	960
Psp124BI	GAGCTC	1 cut(s)	621
Psp6I	CCWGG	4 cut(s)	151, 482, 558, 1038
PspFI	CCCAGC	1 cut(s)	365
PspGI	CCWGG	4 cut(s)	151, 482, 558, 1038
PspLI	CGTACG	1 cut(s)	960
PspN4I	GGNNCC	1 cut(s)	415
PspPI	GGNCC	2 cut(s)	861, 1042
PspXI	VCTCGAGB	1 cut(s)	620
PstI	CTGCAG	1 cut(s)	662
PsuI	RGATCY	1 cut(s)	533
RsaI	GTAC	2 cut(s)	962, 1079
RsaNI	GTAC	2 cut(s)	961, 1078
RseI	CAYNNNNRTG	1 cut(s)	757
SacI	GAGCTC	1 cut(s)	621
SapI	GCTCTTC	1 cut(s)	960
SaqAI	TTAA	2 cut(s)	213, 297
SatI	GCNGC	9 cut(s)	18, 370, 472, 565, 658, 691, 885, 1060, 1092
Sau3AI	GATC	4 cut(s)	244, 533, 612, 1140
Sau96I	GGNCC	2 cut(s)	861, 1042
ScrFI	CCNGG	5 cut(s)	153, 484, 509, 560, 1040
SduI	GDGCHC	3 cut(s)	509, 621, 803
SfcI	CTRYAG	1 cut(s)	658
Sfr274I	CTCGAG	1 cut(s)	620
SgrAI	CRCCGGYG	1 cut(s)	949
SinI	GGWCC	1 cut(s)	861
SlaI	CTCGAG	1 cut(s)	620
SmiMI	CAYNNNNRTG	1 cut(s)	757
SmlI	CTYRAG	2 cut(s)	620, 636
SmoI	CTYRAG	2 cut(s)	620, 636
Sse9I	AATT	5 cut(s)	294, 466, 697, 719, 989
SseBI	AGGCCT	1 cut(s)	1106
SspMI	CTAG	4 cut(s)	318, 324, 330, 537
SstI	GAGCTC	1 cut(s)	621
StuI	AGGCCT	1 cut(s)	1106
StyD4I	CCNGG	5 cut(s)	151, 482, 507, 558, 1038
StyI	CCWWGG	1 cut(s)	758
TaaI	ACNGT	2 cut(s)	413, 530
TaiI	ACGT	1 cut(s)	962
TaqI	TCGA	5 cut(s)	290, 615, 621, 993, 1171
TaqII	GACCGA	1 cut(s)	793
TasI	AATT	5 cut(s)	294, 466, 697, 719, 989
TauI	GCSGC	5 cut(s)	372, 693, 887, 1062, 1094
TfiI	GAWTC	2 cut(s)	641, 974
Tru1I	TTAA	2 cut(s)	213, 297
Tru9I	TTAA	2 cut(s)	213, 297
TscAI	CASTG	1 cut(s)	819
TseFI	GTSAC	2 cut(s)	711, 814
TseI	GCWGC	4 cut(s)	17, 471, 564, 657
Tsp45I	GTSAC	2 cut(s)	711, 814
TspRI	CASTG	1 cut(s)	819
VpaK11BI	GGWCC	1 cut(s)	861
XapI	RAATTY	1 cut(s)	989
XhoI	CTCGAG	1 cut(s)	620
XmiI	GTMKAC	1 cut(s)	1035
XspI	CTAG	4 cut(s)	318, 324, 330, 537

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.