Rmu_sc0000332.1_g000062

starch synthase 4, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000332.1
OG0018725
Physical Location & Seq
Reverse (-)
295037 .. 301038
6002 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2040 bp
atgaaggcggcgttgatcagcaccgccattccttggcagcctccgccgcaccataagcctctcaaacccctttgttgctccaaggttaacggagcctcctcctcgccgtcgccgtcgccgtcatcctcctcacaaatcaatttcaggttgcaaggcagcgaaaatgctgagctccctcagcctcggattcagtctggggaagcgaaggagactgacgtttggcgactcttcagagaagctcagaagaatattctgcatttgaaccagcatcggcttaaggccgtagaggatctgaacaaaacaaacagtgagaagcagctgctgattgataagattgagcagttggagcgggaaaagcaggcttctgttggaaagcctcaagataggctctcaagttgttgggaagtactccttcggatagactcaatggtcctcactagaatgattgctgccggggaggcatctgatttgagaaggctggtcatggatcataaagttagtctagctgaagtttttgatggcaccttgcagaagacagatgccgagcttctagctgaactgagacacttctttgcaagaagccgaaggaatggctttcacattgtgcacatatgtacggaaatggcaccactggtctctgttggatctttggcatcatatgtgactggcttgtctcgtgcactacagaggaaggggcatttggtggaggtgatacttccaaagtattcaagcctggacctagatgaaattcaagggctacaagagattgaggcagagtccttttcatatttcaatggtcaacttcatggaaacagaatttggactggtgttgtctatggcattggagttaccttaattcaacctctagagtattcagagtttttcaatcgtgaaaaggtgtatggctactcagatgactttgaaaggtttacctatttctctcgtgcttcattagattatattgtaaagtgtggaaagcaacctgatgtgatacatatccacaactgggagactgctattattgggccacttttctgggatatttttgctaaccagggacttgaaggtacgagaatcttattgacatgccatgacctgaattcacagtgcctcgagcatccggataaactagcgttatgcggacttgatccttcttcacttcaccgtcctgatcgcttgcaagataatactaagtcacatttggttaatattttgaagggtggagttgtttactccaataatgttgtcataatgtcttccatactatcaaaaggcagggttatacatactctgagtcatgggttggaccctaccttaaacattcacaagaacaagttggttgtttctccttgtggatttgacaattccacctgggatccatccaaggacagctttcttccccaaaatttcaatgtcgaagatatggaagggaaagcagtttgcaaagctgcattgcagcagcaccttgggttatcggagcatgcttctaccattcttattggatgcattgtgtctgaagatgttgatctggagaacatgagggcagtaatcatgaaagcttgcaagagcgatgtacagttcatcatcatgggtactagcaaattatcaagtatacacaagattggatcattgcaggaaccactgaaggatgaacaagtgaaattcgtaaatgagtgtgatgcagcaataacacatttggtttttgccggatctgatattatcttgtgccaatcttttcatgaccctgttctccaagttccattaaaggctttaaagtacggagctgctccaattgcattaaactcctcccatgacaacttcaggacctttgcagaacatgactatcagactactaatttctcacgtttcatcagctctacctttggaaatatgtccataagcgaagtgctggatgagatgaaaaaccacccctcgaagtggaagagaaaaatactggacgccatggaaatggacttctcatgggatgctgagtgctgtgacgtccactgttctgcttacactgctataaagaaatcatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko01110 ko02026 map00500 map01100 map01110 map02026
Pfam Domains
Protein Families
Glyco_transf_5

Protein Analysis

679

Amino Acids

76.11

Weight (kDa)

6.28

Isoelectric Point (pI)

46.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_5 PF08323 200 - 422 1.2e-39 Starch synthase catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018725)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66595
rosa_chinensis RchiOBHm_Chr2g0109671
rosa_multiflora Rmu_sc0000332.1_g000062 Rmu_sc0027285.1_g000001
rosa_rugosa Rorug02G0161800
rosa_samantha Rh2AG214100 Rh2BG224400 Rh2CG216400 Rh2DG219700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 428, 670
AatII GACGTC 1 cut(s) 2004
AccB1I GGYRCC 2 cut(s) 521, 625
AccB7I CCANNNNNTGG 1 cut(s) 33
AccBSI CCGCTC 1 cut(s) 349
AccI GTMKAC 1 cut(s) 1612
AccIII TCCGGA 1 cut(s) 1120
AciI CCGC 6 cut(s) 8, 24, 44, 47, 349, 1140
AclWI GGATC 8 cut(s) 297, 495, 652, 1142, 1370, 1383, 1633, 1717
AcsI RAATTY 5 cut(s) 747, 816, 1099, 1405, 1661
AcuI CTGAAG 5 cut(s) 214, 528, 1536, 1664, 1804
AcyI GRCGYC 2 cut(s) 1959, 2001
AdeI CACNNNGTG 1 cut(s) 604
AfaI GTAC 6 cut(s) 408, 616, 1069, 1575, 1594, 1778
AfiI CCNNNNNNNGG 3 cut(s) 33, 1006, 1938
AflII CTTAAG 1 cut(s) 275
AjnI CCWGG 3 cut(s) 732, 1053, 1370
AjuI GAANNNNNNNTTGG 4 cut(s) 683, 715, 802, 834
Alw21I GWGCWC 3 cut(s) 174, 609, 682
Alw26I GTCTC 5 cut(s) 203, 556, 640, 678, 1004
Alw44I GTGCAC 2 cut(s) 605, 678
AlwI GGATC 8 cut(s) 297, 495, 652, 1142, 1370, 1383, 1633, 1717
AlwNI CAGNNNCTG 1 cut(s) 322
Ama87I CYCGRG 1 cut(s) 1112
Aor13HI TCCGGA 1 cut(s) 1120
AoxI GGCC 2 cut(s) 279, 1025
ApaLI GTGCAC 2 cut(s) 605, 678
ApoI RAATTY 5 cut(s) 747, 816, 1099, 1405, 1661
AspS9I GGNCC 5 cut(s) 430, 736, 1025, 1306, 1824
AsuC2I CCSGG 1 cut(s) 454
AsuHPI GGTGA 2 cut(s) 721, 1154
AvaI CYCGRG 1 cut(s) 1112
AvaII GGWCC 4 cut(s) 430, 736, 1306, 1824
BaeGI GKGCMC 2 cut(s) 609, 682
BamHI GGATCC 1 cut(s) 1375
BanI GGYRCC 2 cut(s) 521, 625
BanII GRGCYC 1 cut(s) 174
BauI CACGAG 2 cut(s) 675, 942
BbsI GAAGAC 2 cut(s) 539, 1248
Bbv12I GWGCWC 3 cut(s) 174, 609, 682
BbvCI CCTCAGC 1 cut(s) 177
BccI CCATC 2 cut(s) 512, 1387
BceAI ACGGC 4 cut(s) 91, 97, 103, 266
BciT130I CCWGG 3 cut(s) 734, 1055, 1372
BclI TGATCA 1 cut(s) 15
BcnI CCSGG 1 cut(s) 454
BcoDI GTCTC 5 cut(s) 203, 556, 640, 678, 1004
BfaI CTAG 7 cut(s) 438, 503, 551, 740, 866, 1130, 1596
BfmI CTRYAG 1 cut(s) 683
BfrI CTTAAG 1 cut(s) 275
BglI GCCNNNNNGGC 1 cut(s) 458
BlpI GCTNAGC 1 cut(s) 168
BmcAI AGTACT 1 cut(s) 408
Bme1390I CCNGG 4 cut(s) 454, 734, 1055, 1372
Bme18I GGWCC 4 cut(s) 430, 736, 1306, 1824
BmeT110I CYCGRG 1 cut(s) 1112
BmgT120I GGNCC 5 cut(s) 430, 736, 1025, 1306, 1824
BmiI GGNNCC 6 cut(s) 94, 523, 627, 1308, 1377, 1638
BmrFI CCNGG 4 cut(s) 454, 734, 1055, 1372
BmrI ACTGGG 1 cut(s) 1015
BmsI GCATC 8 cut(s) 277, 470, 529, 662, 1126, 1493, 1669, 1975
BmuI ACTGGG 1 cut(s) 1015
BpiI GAAGAC 2 cut(s) 539, 1248
BpmI CTGGAG 1 cut(s) 1550
Bpu10I CCTNAGC 1 cut(s) 177
Bpu1102I GCTNAGC 1 cut(s) 168
BpuEI CTTGAG 2 cut(s) 363, 376
BpuMI CCSGG 1 cut(s) 454
BsaBI GATNNNNATC 2 cut(s) 995, 1524
BsaHI GRCGYC 2 cut(s) 1959, 2001
BsaI GGTCTC 1 cut(s) 640
BsaJI CCNNGG 9 cut(s) 32, 81, 182, 453, 1054, 1371, 1383, 1465, 1962
BsaWI WCCGGW 1 cut(s) 1120
BsaXI ACNNNNNCTCC 2 cut(s) 80, 110
Bsc4I CCNNNNNNNGG 3 cut(s) 33, 1006, 1938
Bse1I ACTGG 5 cut(s) 636, 670, 829, 1010, 1959
Bse3DI GCAATG 2 cut(s) 1451, 1628
Bse8I GATNNNNATC 2 cut(s) 995, 1524
BseAI TCCGGA 1 cut(s) 1120
BseBI CCWGG 3 cut(s) 734, 1055, 1372
BseDI CCNNGG 9 cut(s) 32, 81, 182, 453, 1054, 1371, 1383, 1465, 1962
BseGI GGATG 7 cut(s) 122, 1117, 1379, 1508, 1654, 1918, 1990
BseJI GATNNNNATC 2 cut(s) 995, 1524
BseLI CCNNNNNNNGG 3 cut(s) 33, 1006, 1938
BseMI GCAATG 2 cut(s) 1451, 1628
BseMII CTCAG 7 cut(s) 159, 191, 254, 551, 924, 1283, 1980
BseNI ACTGG 5 cut(s) 636, 670, 829, 1010, 1959
BseRI GAGGAG 4 cut(s) 88, 91, 118, 1795
BseSI GKGCMC 2 cut(s) 609, 682
BshFI GGCC 2 cut(s) 281, 1027
BshNI GGYRCC 2 cut(s) 521, 625
BsiHKAI GWGCWC 3 cut(s) 174, 609, 682
BsiHKCI CYCGRG 1 cut(s) 1112
BsiSI CCGG 3 cut(s) 453, 1121, 1707
BslFI GGGAC 1 cut(s) 1071
BslI CCNNNNNNNGG 3 cut(s) 33, 1006, 1938
BsmAI GTCTC 5 cut(s) 203, 556, 640, 678, 1004
BsmFI GGGAC 1 cut(s) 1071
BsnI GGCC 2 cut(s) 281, 1027
Bso31I GGTCTC 1 cut(s) 640
BsoBI CYCGRG 1 cut(s) 1112
Bsp1286I GDGCHC 3 cut(s) 174, 609, 682
Bsp13I TCCGGA 1 cut(s) 1120
Bsp1407I TGTACA 1 cut(s) 1573
Bsp1720I GCTNAGC 1 cut(s) 168
Bsp19I CCATGG 1 cut(s) 1962
BspACI CCGC 6 cut(s) 8, 24, 44, 47, 349, 1140
BspANI GGCC 2 cut(s) 281, 1027
BspCNI CTCAG 7 cut(s) 160, 190, 253, 552, 923, 1284, 1981
BspEI TCCGGA 1 cut(s) 1120
BspHI TCATGA 3 cut(s) 1551, 1738, 2036
BspLI GGNNCC 6 cut(s) 94, 523, 627, 1308, 1377, 1638
BspPI GGATC 8 cut(s) 297, 495, 652, 1142, 1370, 1383, 1633, 1717
BspT107I GGYRCC 2 cut(s) 521, 625
BspTI CTTAAG 1 cut(s) 275
BspTNI GGTCTC 1 cut(s) 640
BsrBI CCGCTC 1 cut(s) 349
BsrDI GCAATG 2 cut(s) 1451, 1628
BsrGI TGTACA 1 cut(s) 1573
BsrI ACTGG 5 cut(s) 636, 670, 829, 1010, 1959
BssECI CCNNGG 9 cut(s) 32, 81, 182, 453, 1054, 1371, 1383, 1465, 1962
BssNAI GTATAC 1 cut(s) 1613
BssNI GRCGYC 2 cut(s) 1959, 2001
BssSI CACGAG 2 cut(s) 675, 942
BssT1I CCWWGG 5 cut(s) 32, 81, 1383, 1465, 1962
Bst1107I GTATAC 1 cut(s) 1613
Bst2BI CACGAG 2 cut(s) 675, 942
Bst2UI CCWGG 3 cut(s) 734, 1055, 1372
Bst4CI ACNGT 5 cut(s) 308, 1107, 1166, 1578, 2009
Bst6I CTCTTC 2 cut(s) 233, 1937
BstACI GRCGYC 2 cut(s) 1959, 2001
BstAFI CTTAAG 1 cut(s) 275
BstAUI TGTACA 1 cut(s) 1573
BstC8I GCNNGC 4 cut(s) 360, 1178, 1482, 1561
BstDEI CTNAG 8 cut(s) 168, 177, 240, 560, 910, 1191, 1292, 1989
BstDSI CCRYGG 1 cut(s) 1962
BstF5I GGATG 7 cut(s) 122, 1117, 1379, 1508, 1654, 1918, 1990
BstMAI GTCTC 5 cut(s) 203, 556, 640, 678, 1004
BstMWI GCNNNNNNNGC 9 cut(s) 43, 46, 55, 178, 346, 355, 458, 1793, 2021
BstNI CCWGG 3 cut(s) 734, 1055, 1372
BstNSI RCATGY 2 cut(s) 1089, 1484
BstSCI CCNGG 4 cut(s) 452, 732, 1053, 1370
BstSFI CTRYAG 1 cut(s) 683
BstSLI GKGCMC 2 cut(s) 609, 682
BstV2I GAAGAC 2 cut(s) 539, 1248
BstX2I RGATCY 4 cut(s) 289, 644, 1375, 1709
BstXI CCANNNNNNTGG 2 cut(s) 1035, 1969
BstYI RGATCY 4 cut(s) 289, 644, 1375, 1709
BstZ17I GTATAC 1 cut(s) 1613
BsuRI GGCC 2 cut(s) 281, 1027
BtgI CCRYGG 1 cut(s) 1962
BtgZI GCGATG 1 cut(s) 1584
BtsCI GGATG 7 cut(s) 122, 1117, 1379, 1508, 1654, 1918, 1990
BtsI GCAGTG 1 cut(s) 2019
BtsIMutI CAGTG 6 cut(s) 313, 629, 1112, 1640, 2005, 2019
Cac8I GCNNGC 4 cut(s) 360, 1178, 1482, 1561
CaiI CAGNNNCTG 1 cut(s) 322
CciI TCATGA 3 cut(s) 1551, 1738, 2036
Cfr13I GGNCC 5 cut(s) 430, 736, 1025, 1306, 1824
CseI GACGC 1 cut(s) 1967
Csp6I GTAC 6 cut(s) 407, 615, 1068, 1574, 1593, 1777
CviQI GTAC 6 cut(s) 407, 615, 1068, 1574, 1593, 1777
DdeI CTNAG 8 cut(s) 168, 177, 240, 560, 910, 1191, 1292, 1989
DraI TTTAAA 1 cut(s) 1773
DraIII CACNNNGTG 1 cut(s) 604
DrdI GACNNNNNNGTC 2 cut(s) 428, 670
DseDI GACNNNNNNGTC 2 cut(s) 428, 670
Eam1104I CTCTTC 2 cut(s) 233, 1937
EarI CTCTTC 2 cut(s) 233, 1937
EciI GGCGGA 1 cut(s) 33
Ecl136II GAGCTC 1 cut(s) 172
Eco130I CCWWGG 5 cut(s) 32, 81, 1383, 1465, 1962
Eco24I GRGCYC 1 cut(s) 174
Eco31I GGTCTC 1 cut(s) 640
Eco47I GGWCC 4 cut(s) 430, 736, 1306, 1824
Eco53kI GAGCTC 1 cut(s) 172
Eco57I CTGAAG 5 cut(s) 214, 528, 1536, 1664, 1804
Eco88I CYCGRG 1 cut(s) 1112
EcoICRI GAGCTC 1 cut(s) 172
EcoO109I RGGNCCY 1 cut(s) 1824
EcoRI GAATTC 1 cut(s) 1099
EcoRII CCWGG 3 cut(s) 732, 1053, 1370
EcoT14I CCWWGG 5 cut(s) 32, 81, 1383, 1465, 1962
EcoT22I ATGCAT 1 cut(s) 1508
EcoT38I GRGCYC 1 cut(s) 174
ErhI CCWWGG 5 cut(s) 32, 81, 1383, 1465, 1962
FalI AAGNNNNNCTT 4 cut(s) 396, 428, 1376, 1408
FaqI GGGAC 1 cut(s) 1071
FauI CCCGC 1 cut(s) 342
FauNDI CATATG 2 cut(s) 611, 658
FbaI TGATCA 1 cut(s) 15
FblI GTMKAC 1 cut(s) 1612
FokI GGATG 7 cut(s) 109, 1104, 1366, 1515, 1661, 1925, 1997
FriOI GRGCYC 1 cut(s) 174
FspBI CTAG 7 cut(s) 438, 503, 551, 740, 866, 1130, 1596
GsuI CTGGAG 1 cut(s) 1550
HaeIII GGCC 2 cut(s) 281, 1027
HapII CCGG 3 cut(s) 453, 1121, 1707
HgaI GACGC 1 cut(s) 1967
Hin1I GRCGYC 2 cut(s) 1959, 2001
HincII GTYRAC 2 cut(s) 88, 800
HindII GTYRAC 2 cut(s) 88, 800
HindIII AAGCTT 1 cut(s) 1557
HinfI GANTC 6 cut(s) 187, 225, 422, 776, 1074, 1294
HpaI GTTAAC 1 cut(s) 88
HpaII CCGG 3 cut(s) 453, 1121, 1707
HphI GGTGA 2 cut(s) 721, 1154
Hpy166II GTNNAC 8 cut(s) 88, 607, 680, 800, 930, 1231, 1613, 2005
Hpy8I GTNNAC 8 cut(s) 88, 607, 680, 800, 930, 1231, 1613, 2005
Hpy99I CGWCG 2 cut(s) 112, 118
HpyCH4III ACNGT 5 cut(s) 308, 1107, 1166, 1578, 2009
HpyCH4IV ACGT 3 cut(s) 216, 1864, 2001
HpyF10VI GCNNNNNNNGC 9 cut(s) 43, 46, 55, 178, 346, 355, 458, 1793, 2021
HpyF3I CTNAG 8 cut(s) 168, 177, 240, 560, 910, 1191, 1292, 1989
HpySE526I ACGT 3 cut(s) 216, 1864, 2001
Hsp92I GRCGYC 2 cut(s) 1959, 2001
Kpn2I TCCGGA 1 cut(s) 1120
Ksp22I TGATCA 1 cut(s) 15
KspAI GTTAAC 1 cut(s) 88
LmnI GCTCC 7 cut(s) 83, 92, 177, 346, 1477, 1781, 1792
LweI GCATC 8 cut(s) 277, 470, 529, 662, 1126, 1493, 1669, 1975
MaeI CTAG 7 cut(s) 438, 503, 551, 740, 866, 1130, 1596
MaeII ACGT 3 cut(s) 216, 1864, 2001
MaeIII GTNAC 4 cut(s) 661, 847, 1194, 1997
MbiI CCGCTC 1 cut(s) 349
MboII GAAGA 9 cut(s) 220, 256, 544, 1146, 1248, 1388, 1430, 1529, 1954
MfeI CAATTG 1 cut(s) 1791
MflI RGATCY 4 cut(s) 289, 644, 1375, 1709
MhlI GDGCHC 3 cut(s) 174, 609, 682
MlyI GAGTC 4 cut(s) 219, 416, 785, 1303
MmeI TCCRAC 4 cut(s) 324, 349, 622, 1284
Mph1103I ATGCAT 1 cut(s) 1508
MroI TCCGGA 1 cut(s) 1120
MseI TTAA 8 cut(s) 87, 276, 854, 1206, 1316, 1763, 1772, 1799
MslI CAYNNNNRTG 3 cut(s) 440, 1586, 1967
MspA1I CMGCKG 1 cut(s) 319
MspCI CTTAAG 1 cut(s) 275
MspI CCGG 3 cut(s) 453, 1121, 1707
MspR9I CCNGG 4 cut(s) 454, 734, 1055, 1372
MunI CAATTG 1 cut(s) 1791
MvaI CCWGG 3 cut(s) 734, 1055, 1372
MwoI GCNNNNNNNGC 9 cut(s) 43, 46, 55, 178, 346, 355, 458, 1793, 2021
NciI CCSGG 1 cut(s) 454
NcoI CCATGG 1 cut(s) 1962
NdeI CATATG 2 cut(s) 611, 658
NlaIV GGNNCC 6 cut(s) 94, 523, 627, 1308, 1377, 1638
NmeAIII GCCGAG 1 cut(s) 568
NmuCI GTSAC 3 cut(s) 661, 1194, 1997
NsiI ATGCAT 1 cut(s) 1508
NspI RCATGY 2 cut(s) 1089, 1484
PaeI GCATGC 1 cut(s) 1484
PaeR7I CTCGAG 1 cut(s) 1112
PagI TCATGA 3 cut(s) 1551, 1738, 2036
PcsI WCGNNNNNNNCGW 2 cut(s) 110, 116
PfeI GAWTC 2 cut(s) 187, 1074
PflMI CCANNNNNTGG 1 cut(s) 33
PleI GAGTC 4 cut(s) 219, 416, 784, 1302
PpsI GAGTC 4 cut(s) 219, 416, 784, 1302
PpuMI RGGWCCY 1 cut(s) 1824
Psp124BI GAGCTC 1 cut(s) 174
Psp5II RGGWCCY 1 cut(s) 1824
Psp6I CCWGG 3 cut(s) 732, 1053, 1370
PspGI CCWGG 3 cut(s) 732, 1053, 1370
PspN4I GGNNCC 6 cut(s) 94, 523, 627, 1308, 1377, 1638
PspPI GGNCC 5 cut(s) 430, 736, 1025, 1306, 1824
PspPPI RGGWCCY 1 cut(s) 1824
PspXI VCTCGAGB 1 cut(s) 1112
PstNI CAGNNNCTG 1 cut(s) 322
PsuI RGATCY 4 cut(s) 289, 644, 1375, 1709
PvuII CAGCTG 1 cut(s) 319
RsaI GTAC 6 cut(s) 408, 616, 1069, 1575, 1594, 1778
RsaNI GTAC 6 cut(s) 407, 615, 1068, 1574, 1593, 1777
RseI CAYNNNNRTG 3 cut(s) 440, 1586, 1967
SacI GAGCTC 1 cut(s) 174
SaqAI TTAA 8 cut(s) 87, 276, 854, 1206, 1316, 1763, 1772, 1799
Sau96I GGNCC 5 cut(s) 430, 736, 1025, 1306, 1824
ScaI AGTACT 1 cut(s) 408
SchI GAGTC 4 cut(s) 219, 416, 785, 1303
ScrFI CCNGG 4 cut(s) 454, 734, 1055, 1372
SduI GDGCHC 3 cut(s) 174, 609, 682
SfaNI GCATC 8 cut(s) 277, 470, 529, 662, 1126, 1493, 1669, 1975
SfcI CTRYAG 1 cut(s) 683
Sfr274I CTCGAG 1 cut(s) 1112
SinI GGWCC 4 cut(s) 430, 736, 1306, 1824
SlaI CTCGAG 1 cut(s) 1112
SmiMI CAYNNNNRTG 3 cut(s) 440, 1586, 1967
SmlI CTYRAG 4 cut(s) 275, 378, 391, 1112
SmoI CTYRAG 4 cut(s) 275, 378, 391, 1112
SphI GCATGC 1 cut(s) 1484
SsiI CCGC 6 cut(s) 8, 24, 44, 47, 349, 1140
SspI AATATT 2 cut(s) 250, 1210
SspMI CTAG 7 cut(s) 438, 503, 551, 740, 866, 1130, 1596
SstI GAGCTC 1 cut(s) 174
StyD4I CCNGG 4 cut(s) 452, 732, 1053, 1370
StyI CCWWGG 5 cut(s) 32, 81, 1383, 1465, 1962
TaaI ACNGT 5 cut(s) 308, 1107, 1166, 1578, 2009
TaiI ACGT 3 cut(s) 219, 1867, 2004
TaqI TCGA 3 cut(s) 1113, 1416, 1934
TatI WGTACW 2 cut(s) 406, 1573
TauI GCSGC 2 cut(s) 11, 49
TfiI GAWTC 2 cut(s) 187, 1074
Tru1I TTAA 8 cut(s) 87, 276, 854, 1206, 1316, 1763, 1772, 1799
Tru9I TTAA 8 cut(s) 87, 276, 854, 1206, 1316, 1763, 1772, 1799
TscAI CASTG 6 cut(s) 313, 636, 1112, 1647, 2012, 2026
TseFI GTSAC 3 cut(s) 661, 1194, 1997
Tsp45I GTSAC 3 cut(s) 661, 1194, 1997
TspGWI ACGGA 3 cut(s) 105, 632, 1794
TspRI CASTG 6 cut(s) 313, 636, 1112, 1647, 2012, 2026
Van91I CCANNNNNTGG 1 cut(s) 33
Vha464I CTTAAG 1 cut(s) 275
VneI GTGCAC 2 cut(s) 605, 678
VpaK11BI GGWCC 4 cut(s) 430, 736, 1306, 1824
XapI RAATTY 5 cut(s) 747, 816, 1099, 1405, 1661
XbaI TCTAGA 1 cut(s) 865
XceI RCATGY 2 cut(s) 1089, 1484
XhoI CTCGAG 1 cut(s) 1112
XmiI GTMKAC 1 cut(s) 1612
XspI CTAG 7 cut(s) 438, 503, 551, 740, 866, 1130, 1596
ZraI GACGTC 1 cut(s) 2002
ZrmI AGTACT 1 cut(s) 408
Zsp2I ATGCAT 1 cut(s) 1508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.