Rmu_sc0000448.1_g000002

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000448.1
OG0020063
Physical Location & Seq
Forward (+)
16042 .. 18809
2768 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000448.1_g000002.1.cds

Sequence Viewer

Length: 792 bp
atgactgcaaacctcgactccttgatcacagtcaagagctctaaagaaagagttgtgcctctttcaagttgctggtccctgaaaacattgactcctcgcctcttcaatgcacggtacaggtggtctgtgaaacccttgcgggtttctccggtcctcccaaccttttcttattggaaacctctgacccgcaagtcgtttgatccaaagacagaatttagggttcaagaatgggttggagatttattgttgttctcctttgaaccagtgtgtgaaaccaacaaggtacttcgaggtgggggttggtgttttgatcgagccctggttttctttgaagaatatgacagtgtcaaacctcttcatgaagatcttgcagttattgccctcaatcccaggaacccgaaaccgacggcgccacccccagaaccggccggaatccacctgaaccggccgttggaagtaactgaaccggcctcaaagaagaatcctttggtttcctacctggttcacatcctttcggaccacttttcacagccatcttctgtcagaagttgcaacaccactccagattccgggaaccggaaaaatccttcccggaaccggccaccaaaatcccgaaccggccagcagtatcactgcatccttgaaccaccaccgagctgccctattctttgttccagtcagcccaagacagtaacaaagcccagaggaaaacagaagcccagaagcccagttgcaacagaagcccaaaagcccacgtgccaagaagaaagggaacagcacctggcccactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

263

Amino Acids

29.77

Weight (kDa)

9.58

Isoelectric Point (pI)

58.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 190
AccB1I GGYRCC 1 cut(s) 409
AciI CCGC 2 cut(s) 139, 187
AclWI GGATC 1 cut(s) 194
AcoI YGGCCR 4 cut(s) 426, 446, 599, 619
AcsI RAATTY 1 cut(s) 212
AcvI CACGTG 1 cut(s) 756
AcyI GRCGYC 1 cut(s) 410
AfaI GTAC 2 cut(s) 116, 285
AfiI CCNNNNNNNGG 5 cut(s) 424, 451, 569, 576, 597
AgsI TTSAA 6 cut(s) 66, 106, 224, 260, 332, 644
AjnI CCWGG 4 cut(s) 318, 389, 498, 780
AjuI GAANNNNNNNTTGG 4 cut(s) 434, 466, 470, 502
AluBI AGCT 2 cut(s) 39, 657
AluI AGCT 2 cut(s) 39, 657
Alw21I GWGCWC 1 cut(s) 41
AlwI GGATC 1 cut(s) 194
AlwNI CAGNNNCTG 1 cut(s) 781
AoxI GGCC 6 cut(s) 426, 446, 468, 599, 619, 783
ApeKI GCWGC 1 cut(s) 657
ApoI RAATTY 1 cut(s) 212
AspLEI GCGC 1 cut(s) 412
AspS9I GGNCC 4 cut(s) 75, 151, 517, 784
AsuC2I CCSGG 2 cut(s) 571, 592
AvaII GGWCC 3 cut(s) 75, 151, 517
BanI GGYRCC 1 cut(s) 409
BanII GRGCYC 2 cut(s) 41, 319
BbrPI CACGTG 1 cut(s) 756
Bbv12I GWGCWC 1 cut(s) 41
BbvI GCAGC 1 cut(s) 644
BccI CCATC 1 cut(s) 541
BceAI ACGGC 2 cut(s) 423, 433
BciT130I CCWGG 4 cut(s) 320, 391, 500, 782
BclI TGATCA 1 cut(s) 24
BcnI CCSGG 2 cut(s) 571, 592
BfoI RGCGCY 1 cut(s) 413
BglII AGATCT 1 cut(s) 364
BisI GCNGC 1 cut(s) 658
BlsI GCNGC 1 cut(s) 659
Bme1390I CCNGG 6 cut(s) 320, 391, 500, 571, 592, 782
Bme18I GGWCC 3 cut(s) 75, 151, 517
BmgT120I GGNCC 4 cut(s) 75, 151, 517, 784
BmiI GGNNCC 5 cut(s) 77, 395, 411, 575, 596
BmrFI CCNGG 6 cut(s) 320, 391, 500, 571, 592, 782
BmrI ACTGGG 1 cut(s) 722
BmsI GCATC 1 cut(s) 645
BmuI ACTGGG 1 cut(s) 722
BpmI CTGGAG 1 cut(s) 546
BpuMI CCSGG 2 cut(s) 571, 592
BsaAI YACGTR 1 cut(s) 756
BsaHI GRCGYC 1 cut(s) 410
BsaJI CCNNGG 2 cut(s) 318, 389
BsaWI WCCGGW 2 cut(s) 148, 576
BsaXI ACNNNNNCTCC 3 cut(s) 32, 76, 106
Bsc4I CCNNNNNNNGG 5 cut(s) 424, 451, 569, 576, 597
Bse118I RCCGGY 5 cut(s) 424, 444, 466, 597, 617
Bse1I ACTGG 3 cut(s) 263, 675, 728
BseBI CCWGG 4 cut(s) 320, 391, 500, 782
BseDI CCNNGG 2 cut(s) 318, 389
BseGI GGATG 2 cut(s) 507, 636
BseLI CCNNNNNNNGG 5 cut(s) 424, 451, 569, 576, 597
BseNI ACTGG 3 cut(s) 263, 675, 728
BseRI GAGGAG 1 cut(s) 84
BseX3I CGGCCG 2 cut(s) 426, 446
BseXI GCAGC 1 cut(s) 644
Bsh1285I CGRYCG 2 cut(s) 429, 449
BshFI GGCC 6 cut(s) 428, 448, 470, 601, 621, 785
BshNI GGYRCC 1 cut(s) 409
BsiEI CGRYCG 2 cut(s) 429, 449
BsiHKAI GWGCWC 1 cut(s) 41
BslFI GGGAC 1 cut(s) 61
BslI CCNNNNNNNGG 5 cut(s) 424, 451, 569, 576, 597
BsmFI GGGAC 1 cut(s) 61
BsnI GGCC 6 cut(s) 428, 448, 470, 601, 621, 785
Bsp1286I GDGCHC 2 cut(s) 41, 319
Bsp143I GATC 4 cut(s) 24, 199, 310, 364
BspACI CCGC 2 cut(s) 139, 187
BspANI GGCC 6 cut(s) 428, 448, 470, 601, 621, 785
BspHI TCATGA 1 cut(s) 358
BspLI GGNNCC 5 cut(s) 77, 395, 411, 575, 596
BspPI GGATC 1 cut(s) 194
BspT107I GGYRCC 1 cut(s) 409
BsrFI RCCGGY 5 cut(s) 424, 444, 466, 597, 617
BsrI ACTGG 3 cut(s) 263, 675, 728
BssAI RCCGGY 5 cut(s) 424, 444, 466, 597, 617
BssECI CCNNGG 2 cut(s) 318, 389
BssMI GATC 4 cut(s) 24, 199, 310, 364
BssNI GRCGYC 1 cut(s) 410
Bst2UI CCWGG 4 cut(s) 320, 391, 500, 782
Bst4CI ACNGT 4 cut(s) 31, 114, 344, 691
Bst6I CTCTTC 2 cut(s) 107, 360
BstACI GRCGYC 1 cut(s) 410
BstAPI GCANNNNNTGC 1 cut(s) 377
BstBAI YACGTR 1 cut(s) 756
BstC8I GCNNGC 1 cut(s) 623
BstF5I GGATG 2 cut(s) 507, 636
BstH2I RGCGCY 1 cut(s) 413
BstHHI GCGC 1 cut(s) 412
BstKTI GATC 4 cut(s) 27, 202, 313, 367
BstMBI GATC 4 cut(s) 24, 199, 310, 364
BstMCI CGRYCG 2 cut(s) 429, 449
BstMWI GCNNNNNNNGC 2 cut(s) 377, 740
BstNI CCWGG 4 cut(s) 320, 391, 500, 782
BstSCI CCNGG 6 cut(s) 318, 389, 498, 569, 590, 780
BstV1I GCAGC 1 cut(s) 644
BstX2I RGATCY 1 cut(s) 364
BstYI RGATCY 1 cut(s) 364
BstZI CGGCCG 2 cut(s) 426, 446
BsuRI GGCC 6 cut(s) 428, 448, 470, 601, 621, 785
BtsCI GGATG 2 cut(s) 507, 636
BtsI GCAGTG 1 cut(s) 631
BtsIMutI CAGTG 4 cut(s) 270, 349, 631, 787
Cac8I GCNNGC 1 cut(s) 623
CaiI CAGNNNCTG 1 cut(s) 781
CciI TCATGA 1 cut(s) 358
CfoI GCGC 1 cut(s) 412
Cfr10I RCCGGY 5 cut(s) 424, 444, 466, 597, 617
Cfr13I GGNCC 4 cut(s) 75, 151, 517, 784
CsiI ACCWGGT 1 cut(s) 498
Csp6I GTAC 2 cut(s) 115, 284
CviAII CATG 1 cut(s) 359
CviQI GTAC 2 cut(s) 115, 284
DinI GGCGCC 1 cut(s) 411
DpnI GATC 4 cut(s) 26, 201, 312, 366
DpnII GATC 4 cut(s) 24, 199, 310, 364
DrdI GACNNNNNNGTC 1 cut(s) 190
DseDI GACNNNNNNGTC 1 cut(s) 190
EaeI YGGCCR 4 cut(s) 426, 446, 599, 619
EagI CGGCCG 2 cut(s) 426, 446
Eam1104I CTCTTC 2 cut(s) 107, 360
EarI CTCTTC 2 cut(s) 107, 360
Ecl136II GAGCTC 1 cut(s) 39
EclXI CGGCCG 2 cut(s) 426, 446
Eco24I GRGCYC 2 cut(s) 41, 319
Eco47I GGWCC 3 cut(s) 75, 151, 517
Eco52I CGGCCG 2 cut(s) 426, 446
Eco53kI GAGCTC 1 cut(s) 39
Eco72I CACGTG 1 cut(s) 756
EcoICRI GAGCTC 1 cut(s) 39
EcoRII CCWGG 4 cut(s) 318, 389, 498, 780
EcoT38I GRGCYC 2 cut(s) 41, 319
EgeI GGCGCC 1 cut(s) 411
EheI GGCGCC 1 cut(s) 411
FaeI CATG 1 cut(s) 362
FaiI YATR 2 cut(s) 339, 360
FaqI GGGAC 1 cut(s) 61
FatI CATG 1 cut(s) 358
FauI CCCGC 2 cut(s) 132, 194
FbaI TGATCA 1 cut(s) 24
Fnu4HI GCNGC 1 cut(s) 658
FokI GGATG 2 cut(s) 494, 623
FriOI GRGCYC 2 cut(s) 41, 319
Fsp4HI GCNGC 1 cut(s) 658
GlaI GCGC 1 cut(s) 411
GluI GCNGC 1 cut(s) 658
GsuI CTGGAG 1 cut(s) 546
HaeII RGCGCY 1 cut(s) 413
HaeIII GGCC 6 cut(s) 428, 448, 470, 601, 621, 785
HhaI GCGC 1 cut(s) 412
Hin1I GRCGYC 1 cut(s) 410
Hin1II CATG 1 cut(s) 362
Hin6I GCGC 1 cut(s) 410
HinP1I GCGC 1 cut(s) 410
HinfI GANTC 5 cut(s) 17, 91, 432, 481, 566
Hpy166II GTNNAC 1 cut(s) 505
Hpy188I TCNGA 3 cut(s) 183, 517, 545
Hpy188III TCNNGA 5 cut(s) 34, 224, 359, 563, 612
Hpy8I GTNNAC 1 cut(s) 505
Hpy99I CGWCG 1 cut(s) 409
HpyAV CCTTC 1 cut(s) 597
HpyCH4III ACNGT 4 cut(s) 31, 114, 344, 691
HpyCH4IV ACGT 1 cut(s) 755
HpyCH4V TGCA 6 cut(s) 8, 110, 371, 552, 636, 734
HpyF10VI GCNNNNNNNGC 2 cut(s) 377, 740
HpySE526I ACGT 1 cut(s) 755
Hsp92I GRCGYC 1 cut(s) 410
Hsp92II CATG 1 cut(s) 362
HspAI GCGC 1 cut(s) 410
KasI GGCGCC 1 cut(s) 409
Ksp22I TGATCA 1 cut(s) 24
Kzo9I GATC 4 cut(s) 24, 199, 310, 364
Lsp1109I GCAGC 1 cut(s) 644
LweI GCATC 1 cut(s) 645
MabI ACCWGGT 1 cut(s) 498
MaeII ACGT 1 cut(s) 755
MaeIII GTNAC 2 cut(s) 457, 691
MalI GATC 4 cut(s) 26, 201, 312, 366
MboI GATC 4 cut(s) 24, 199, 310, 364
MboII GAAGA 7 cut(s) 94, 344, 347, 374, 490, 528, 776
MflI RGATCY 1 cut(s) 364
MhlI GDGCHC 2 cut(s) 41, 319
MluCI AATT 1 cut(s) 212
Mly113I GGCGCC 1 cut(s) 410
MlyI GAGTC 2 cut(s) 11, 85
MmeI TCCRAC 2 cut(s) 214, 432
MspR9I CCNGG 6 cut(s) 320, 391, 500, 571, 592, 782
MvaI CCWGG 4 cut(s) 320, 391, 500, 782
MwoI GCNNNNNNNGC 2 cut(s) 377, 740
NarI GGCGCC 1 cut(s) 410
NciI CCSGG 2 cut(s) 571, 592
NdeII GATC 4 cut(s) 24, 199, 310, 364
NlaIII CATG 1 cut(s) 362
NlaIV GGNNCC 5 cut(s) 77, 395, 411, 575, 596
PagI TCATGA 1 cut(s) 358
PfeI GAWTC 3 cut(s) 432, 481, 566
PflFI GACNNNGTC 1 cut(s) 344
PfoI TCCNGGA 2 cut(s) 569, 590
PkrI GCNGC 1 cut(s) 659
PleI GAGTC 2 cut(s) 11, 85
PluTI GGCGCC 1 cut(s) 413
PmaCI CACGTG 1 cut(s) 756
PmlI CACGTG 1 cut(s) 756
PpsI GAGTC 2 cut(s) 11, 85
Ppu21I YACGTR 1 cut(s) 756
Psp124BI GAGCTC 1 cut(s) 41
Psp6I CCWGG 4 cut(s) 318, 389, 498, 780
PspCI CACGTG 1 cut(s) 756
PspGI CCWGG 4 cut(s) 318, 389, 498, 780
PspN4I GGNNCC 5 cut(s) 77, 395, 411, 575, 596
PspPI GGNCC 4 cut(s) 75, 151, 517, 784
PstNI CAGNNNCTG 1 cut(s) 781
PsuI RGATCY 1 cut(s) 364
PsyI GACNNNGTC 1 cut(s) 344
RsaI GTAC 2 cut(s) 116, 285
RsaNI GTAC 2 cut(s) 115, 284
SacI GAGCTC 1 cut(s) 41
SatI GCNGC 1 cut(s) 658
Sau3AI GATC 4 cut(s) 24, 199, 310, 364
Sau96I GGNCC 4 cut(s) 75, 151, 517, 784
SchI GAGTC 2 cut(s) 11, 85
ScrFI CCNGG 6 cut(s) 320, 391, 500, 571, 592, 782
SduI GDGCHC 2 cut(s) 41, 319
SexAI ACCWGGT 1 cut(s) 498
SfaNI GCATC 1 cut(s) 645
SfoI GGCGCC 1 cut(s) 411
SinI GGWCC 3 cut(s) 75, 151, 517
Sse9I AATT 1 cut(s) 212
SsiI CCGC 2 cut(s) 139, 187
SspDI GGCGCC 1 cut(s) 409
SstI GAGCTC 1 cut(s) 41
StyD4I CCNGG 6 cut(s) 318, 389, 498, 569, 590, 780
TaaI ACNGT 4 cut(s) 31, 114, 344, 691
TaiI ACGT 1 cut(s) 758
TaqI TCGA 3 cut(s) 15, 289, 313
TasI AATT 1 cut(s) 212
TfiI GAWTC 3 cut(s) 432, 481, 566
TscAI CASTG 3 cut(s) 270, 349, 638
TseI GCWGC 1 cut(s) 657
TspDTI ATGAA 2 cut(s) 347, 375
TspRI CASTG 3 cut(s) 270, 349, 638
Tth111I GACNNNGTC 1 cut(s) 344
VpaK11BI GGWCC 3 cut(s) 75, 151, 517
XapI RAATTY 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.