Rmu_sc0000552.1_g000023

WUSCHEL-related homeobox

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000552.1
OG0016616
Physical Location & Seq
Reverse (-)
66610 .. 73250
6641 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1062 bp
atggagcgcgaagatcagcacgtaaagaacggtggattggcacatggagtggggctggagaacacgacgaggtggaatccgaccaaggagcagataactatgctggagaatctgtatgagcaaggggtgaggactcccagtgcggaattcatacaggcggtaacggcgaggctcagggcttatggtcacattgaggggaagaatgtgttttattggtttcagaatcacaaagcgaggcagagacagaaggagaagcaggaggctagcttggcttatttcaaccgctttctccataaggcggcggcctctcagtacccactatatcctcctccccaatatccttgcgcaaatgtgccaagtgaaatagggttctattcacaatctcatcccaaggtgcctccacaatctgttggggtaaagaggagaccaagaattgagaaactggacgtgccaacaagaacttgcagtggcggtggtagtggagggtatgagccaatgcgccatggatatggcagagggggctgtattgatgtcaatgatgccactaacagattaatgagcaactctgatgatccagaaacactgccactgtttcctttgacacccacaggcctcctacagggcagagatgaaggcccatctacttcagatggtaattatgctcagaattcgactgctctcgttagctcttctggtatcgtagagggtcatgagcggcgaggagtcttcttctcagtgcaggctccggttctttctggtcgaggaggcgccgaggccgaggaacaggcaagcttgaccgcccctgcgatgggatgggggactggccggccaccggacacggaggtggaggcagtctggcagctgaagcctagggtttcagccttggtagtttgggtttgggcttgggcctttgggcctgggctcaaacttgggttactggggcaggggcctgcgcttgggctgctgggcctgtttgattttgtttttgtttctgctagacaagactggacttgggcttgggcctacgggcttgggctcgggtccaggccaatggggccctaa

Protein Analysis

353

Amino Acids

38.67

Weight (kDa)

8.35

Isoelectric Point (pI)

42.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 23 - 82 1.5e-12 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016616)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G59340
fragaria_vesca FvH4_3g35730
malus_domestica MD11G1111700.v1.1
prunus_persica Prupe.6G080100_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0063891
rosa_laevigata RLG00000035688
rosa_multiflora Rmu_sc0000552.1_g000023
rosa_roxburghii Rroxscaffold_1G00016880
rosa_rugosa Rorug05G0361000
rosa_samantha Rh5AG419500 Rh5BG435400 Rh5CG458500 Rh5DG448500
rosa_wichuraiana Rw5G039430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 346
AccB1I GGYRCC 2 cut(s) 394, 767
AccBSI CCGCTC 1 cut(s) 715
AccII CGCG 1 cut(s) 9
AciI CCGC 8 cut(s) 143, 158, 283, 299, 302, 471, 715, 798
AclWI GGATC 1 cut(s) 566
AcoI YGGCCR 2 cut(s) 823, 827
AcsI RAATTY 2 cut(s) 146, 667
AcuI CTGAAG 2 cut(s) 630, 884
AcyI GRCGYC 1 cut(s) 768
AfaI GTAC 1 cut(s) 314
AfiI CCNNNNNNNGG 6 cut(s) 298, 619, 808, 809, 832, 956
AgsI TTSAA 1 cut(s) 280
AjiI CACGTC 1 cut(s) 448
AjnI CCWGG 2 cut(s) 916, 1043
AjuI GAANNNNNNNTTGG 2 cut(s) 20, 52
AleI CACNNNNGTG 1 cut(s) 842
AluBI AGCT 4 cut(s) 267, 687, 792, 862
AluI AGCT 4 cut(s) 267, 687, 792, 862
Alw26I GTCTC 2 cut(s) 235, 418
AlwI GGATC 1 cut(s) 566
Ama87I CYCGRG 1 cut(s) 1037
ApaI GGGCCC 1 cut(s) 1059
ApeKI GCWGC 2 cut(s) 859, 961
ApoI RAATTY 2 cut(s) 146, 667
AseI ATTAAT 1 cut(s) 554
AspA2I CCTAGG 1 cut(s) 869
AspLEI GCGC 5 cut(s) 9, 347, 501, 770, 955
AspS9I GGNCC 9 cut(s) 635, 906, 914, 947, 967, 1020, 1041, 1055, 1056
AsuHPI GGTGA 1 cut(s) 139
AsuNHI GCTAGC 1 cut(s) 263
AvaI CYCGRG 1 cut(s) 1037
AvaII GGWCC 1 cut(s) 1041
AvrII CCTAGG 1 cut(s) 869
BaeGI GKGCMC 1 cut(s) 1059
BanI GGYRCC 2 cut(s) 394, 767
BanII GRGCYC 3 cut(s) 924, 1038, 1059
BbsI GAAGAC 1 cut(s) 718
BbvI GCAGC 2 cut(s) 871, 948
BccI CCATC 4 cut(s) 644, 646, 802, 807
BceAI ACGGC 1 cut(s) 180
BciT130I CCWGG 2 cut(s) 918, 1045
BcoDI GTCTC 2 cut(s) 235, 418
BfaI CTAG 3 cut(s) 264, 870, 996
BfmI CTRYAG 1 cut(s) 617
BfoI RGCGCY 1 cut(s) 771
BglI GCCNNNNNGGC 1 cut(s) 1054
BisI GCNGC 5 cut(s) 300, 303, 716, 860, 962
BlnI CCTAGG 1 cut(s) 869
BlsI GCNGC 5 cut(s) 301, 304, 717, 861, 963
Bme1390I CCNGG 2 cut(s) 918, 1045
Bme18I GGWCC 1 cut(s) 1041
BmeT110I CYCGRG 1 cut(s) 1037
BmgBI CACGTC 1 cut(s) 448
BmgT120I GGNCC 9 cut(s) 635, 906, 914, 947, 967, 1020, 1041, 1055, 1056
BmiI GGNNCC 7 cut(s) 396, 744, 769, 948, 1042, 1056, 1057
BmrFI CCNGG 2 cut(s) 918, 1045
BmrI ACTGGG 2 cut(s) 132, 947
BmsI GCATC 1 cut(s) 529
BmtI GCTAGC 1 cut(s) 267
BmuI ACTGGG 2 cut(s) 132, 947
BpiI GAAGAC 1 cut(s) 718
BpmI CTGGAG 2 cut(s) 77, 125
Bpu10I CCTNAGC 1 cut(s) 173
BsaAI YACGTR 1 cut(s) 22
BsaHI GRCGYC 1 cut(s) 768
BsaI GGTCTC 1 cut(s) 418
BsaJI CCNNGG 8 cut(s) 84, 390, 502, 771, 777, 869, 882, 917
BsaWI WCCGGW 2 cut(s) 745, 832
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bsc4I CCNNNNNNNGG 6 cut(s) 298, 619, 808, 809, 832, 956
Bse118I RCCGGY 1 cut(s) 825
Bse1I ACTGG 5 cut(s) 138, 447, 826, 942, 1010
BseBI CCWGG 2 cut(s) 918, 1045
BseDI CCNNGG 8 cut(s) 84, 390, 502, 771, 777, 869, 882, 917
BseGI GGATG 2 cut(s) 385, 818
BseLI CCNNNNNNNGG 6 cut(s) 298, 619, 808, 809, 832, 956
BseMII CTCAG 4 cut(s) 187, 323, 677, 747
BseNI ACTGG 5 cut(s) 138, 447, 826, 942, 1010
BseRI GAGGAG 4 cut(s) 318, 436, 735, 777
BseSI GKGCMC 1 cut(s) 1059
BseXI GCAGC 2 cut(s) 871, 948
BseYI CCCAGC 1 cut(s) 964
BsgI GTGCAG 1 cut(s) 758
Bsh1236I CGCG 1 cut(s) 9
BshNI GGYRCC 2 cut(s) 394, 767
BsiHKCI CYCGRG 1 cut(s) 1037
BsiSI CCGG 3 cut(s) 746, 826, 833
BslFI GGGAC 1 cut(s) 832
BslI CCNNNNNNNGG 6 cut(s) 298, 619, 808, 809, 832, 956
BsmAI GTCTC 2 cut(s) 235, 418
BsmFI GGGAC 1 cut(s) 832
Bso31I GGTCTC 1 cut(s) 418
BsoBI CYCGRG 1 cut(s) 1037
Bsp120I GGGCCC 1 cut(s) 1055
Bsp1286I GDGCHC 3 cut(s) 924, 1038, 1059
Bsp143I GATC 2 cut(s) 13, 571
Bsp19I CCATGG 1 cut(s) 502
BspACI CCGC 8 cut(s) 143, 158, 283, 299, 302, 471, 715, 798
BspCNI CTCAG 4 cut(s) 186, 322, 676, 746
BspFNI CGCG 1 cut(s) 9
BspHI TCATGA 1 cut(s) 709
BspLI GGNNCC 7 cut(s) 396, 744, 769, 948, 1042, 1056, 1057
BspOI GCTAGC 1 cut(s) 267
BspPI GGATC 1 cut(s) 566
BspQI GCTCTTC 1 cut(s) 694
BspT107I GGYRCC 2 cut(s) 394, 767
BspTNI GGTCTC 1 cut(s) 418
BsrBI CCGCTC 1 cut(s) 715
BsrFI RCCGGY 1 cut(s) 825
BsrI ACTGG 5 cut(s) 138, 447, 826, 942, 1010
BssAI RCCGGY 1 cut(s) 825
BssECI CCNNGG 8 cut(s) 84, 390, 502, 771, 777, 869, 882, 917
BssMI GATC 2 cut(s) 13, 571
BssNI GRCGYC 1 cut(s) 768
BssT1I CCWWGG 5 cut(s) 84, 390, 502, 869, 882
Bst2UI CCWGG 2 cut(s) 918, 1045
Bst4CI ACNGT 2 cut(s) 32, 591
Bst6I CTCTTC 1 cut(s) 694
BstACI GRCGYC 1 cut(s) 768
BstBAI YACGTR 1 cut(s) 22
BstC8I GCNNGC 5 cut(s) 265, 741, 790, 827, 951
BstDEI CTNAG 4 cut(s) 173, 309, 663, 733
BstDSI CCRYGG 1 cut(s) 502
BstENI CCTNNNNNAGG 1 cut(s) 617
BstF5I GGATG 2 cut(s) 385, 818
BstFNI CGCG 1 cut(s) 9
BstH2I RGCGCY 1 cut(s) 771
BstHHI GCGC 5 cut(s) 9, 347, 501, 770, 955
BstKTI GATC 2 cut(s) 16, 574
BstMAI GTCTC 2 cut(s) 235, 418
BstMBI GATC 2 cut(s) 13, 571
BstMWI GCNNNNNNNGC 6 cut(s) 164, 269, 519, 865, 961, 1054
BstNI CCWGG 2 cut(s) 918, 1045
BstSCI CCNGG 2 cut(s) 916, 1043
BstSFI CTRYAG 1 cut(s) 617
BstSLI GKGCMC 1 cut(s) 1059
BstUI CGCG 1 cut(s) 9
BstV1I GCAGC 2 cut(s) 871, 948
BstV2I GAAGAC 1 cut(s) 718
BstXI CCANNNNNNTGG 2 cut(s) 509, 1051
BtgI CCRYGG 1 cut(s) 502
BtgZI GCGATG 1 cut(s) 821
BtrI CACGTC 1 cut(s) 448
BtsCI GGATG 2 cut(s) 385, 818
BtsI GCAGTG 2 cut(s) 472, 581
BtsIMutI CAGTG 5 cut(s) 145, 472, 581, 587, 741
Cac8I GCNNGC 5 cut(s) 265, 741, 790, 827, 951
CciI TCATGA 1 cut(s) 709
CfoI GCGC 5 cut(s) 9, 347, 501, 770, 955
Cfr10I RCCGGY 1 cut(s) 825
Cfr13I GGNCC 9 cut(s) 635, 906, 914, 947, 967, 1020, 1041, 1055, 1056
Csp6I GTAC 1 cut(s) 313
CviAII CATG 3 cut(s) 44, 503, 710
CviQI GTAC 1 cut(s) 313
DdeI CTNAG 4 cut(s) 173, 309, 663, 733
DinI GGCGCC 1 cut(s) 769
DpnI GATC 2 cut(s) 15, 573
DpnII GATC 2 cut(s) 13, 571
EaeI YGGCCR 2 cut(s) 823, 827
Eam1104I CTCTTC 1 cut(s) 694
EarI CTCTTC 1 cut(s) 694
Eco130I CCWWGG 5 cut(s) 84, 390, 502, 869, 882
Eco147I AGGCCT 1 cut(s) 612
Eco24I GRGCYC 3 cut(s) 924, 1038, 1059
Eco31I GGTCTC 1 cut(s) 418
Eco47I GGWCC 1 cut(s) 1041
Eco57I CTGAAG 2 cut(s) 630, 884
Eco88I CYCGRG 1 cut(s) 1037
EcoNI CCTNNNNNAGG 1 cut(s) 617
EcoO109I RGGNCCY 3 cut(s) 947, 1055, 1056
EcoRI GAATTC 2 cut(s) 146, 667
EcoRII CCWGG 2 cut(s) 916, 1043
EcoT14I CCWWGG 5 cut(s) 84, 390, 502, 869, 882
EcoT38I GRGCYC 3 cut(s) 924, 1038, 1059
EgeI GGCGCC 1 cut(s) 769
EheI GGCGCC 1 cut(s) 769
ErhI CCWWGG 5 cut(s) 84, 390, 502, 869, 882
FaeI CATG 3 cut(s) 47, 506, 713
FaqI GGGAC 1 cut(s) 832
FatI CATG 3 cut(s) 43, 502, 709
Fnu4HI GCNGC 5 cut(s) 300, 303, 716, 860, 962
FokI GGATG 2 cut(s) 372, 825
FriOI GRGCYC 3 cut(s) 924, 1038, 1059
FseI GGCCGGCC 1 cut(s) 829
Fsp4HI GCNGC 5 cut(s) 300, 303, 716, 860, 962
FspBI CTAG 3 cut(s) 264, 870, 996
FspI TGCGCA 1 cut(s) 346
GlaI GCGC 5 cut(s) 8, 346, 500, 769, 954
GluI GCNGC 5 cut(s) 300, 303, 716, 860, 962
GsaI CCCAGC 1 cut(s) 968
GsuI CTGGAG 2 cut(s) 77, 125
HaeII RGCGCY 1 cut(s) 771
HapII CCGG 3 cut(s) 746, 826, 833
HhaI GCGC 5 cut(s) 9, 347, 501, 770, 955
Hin1I GRCGYC 1 cut(s) 768
Hin1II CATG 3 cut(s) 47, 506, 713
Hin6I GCGC 5 cut(s) 7, 345, 499, 768, 953
HinP1I GCGC 5 cut(s) 7, 345, 499, 768, 953
HindIII AAGCTT 1 cut(s) 790
HinfI GANTC 5 cut(s) 76, 109, 133, 223, 723
HpaII CCGG 3 cut(s) 746, 826, 833
HphI GGTGA 1 cut(s) 139
Hpy188I TCNGA 5 cut(s) 81, 222, 568, 649, 666
Hpy188III TCNNGA 2 cut(s) 575, 710
Hpy99I CGWCG 1 cut(s) 70
HpyAV CCTTC 2 cut(s) 241, 626
HpyCH4III ACNGT 2 cut(s) 32, 591
HpyCH4IV ACGT 2 cut(s) 21, 447
HpyCH4V TGCA 2 cut(s) 465, 739
HpyF10VI GCNNNNNNNGC 6 cut(s) 164, 269, 519, 865, 961, 1054
HpyF3I CTNAG 4 cut(s) 173, 309, 663, 733
HpySE526I ACGT 2 cut(s) 21, 447
Hsp92I GRCGYC 1 cut(s) 768
Hsp92II CATG 3 cut(s) 47, 506, 713
HspAI GCGC 5 cut(s) 7, 345, 499, 768, 953
KasI GGCGCC 1 cut(s) 767
KroI GCCGGC 1 cut(s) 825
KroNI GCCGGC 1 cut(s) 827
Kzo9I GATC 2 cut(s) 13, 571
LguI GCTCTTC 1 cut(s) 694
LmnI GCTCC 3 cut(s) 4, 88, 748
Lsp1109I GCAGC 2 cut(s) 871, 948
LweI GCATC 1 cut(s) 529
MaeI CTAG 3 cut(s) 264, 870, 996
MaeII ACGT 2 cut(s) 21, 447
MaeIII GTNAC 3 cut(s) 160, 185, 933
MalI GATC 2 cut(s) 15, 573
MbiI CCGCTC 1 cut(s) 715
MboI GATC 2 cut(s) 13, 571
MboII GAAGA 5 cut(s) 23, 211, 681, 718, 721
MhlI GDGCHC 3 cut(s) 924, 1038, 1059
MluCI AATT 4 cut(s) 146, 432, 655, 667
Mly113I GGCGCC 1 cut(s) 768
MlyI GAGTC 2 cut(s) 127, 732
MmeI TCCRAC 1 cut(s) 104
MroNI GCCGGC 1 cut(s) 825
MseI TTAA 1 cut(s) 554
MslI CAYNNNNRTG 2 cut(s) 507, 842
MspA1I CMGCKG 1 cut(s) 862
MspI CCGG 3 cut(s) 746, 826, 833
MspR9I CCNGG 2 cut(s) 918, 1045
MvaI CCWGG 2 cut(s) 918, 1045
MvnI CGCG 1 cut(s) 9
MwoI GCNNNNNNNGC 6 cut(s) 164, 269, 519, 865, 961, 1054
NaeI GCCGGC 1 cut(s) 827
NarI GGCGCC 1 cut(s) 768
NcoI CCATGG 1 cut(s) 502
NdeII GATC 2 cut(s) 13, 571
NgoMIV GCCGGC 1 cut(s) 825
NheI GCTAGC 1 cut(s) 263
NlaIII CATG 3 cut(s) 47, 506, 713
NlaIV GGNNCC 7 cut(s) 396, 744, 769, 948, 1042, 1056, 1057
NmeAIII GCCGAG 2 cut(s) 796, 802
NmuCI GTSAC 1 cut(s) 185
NsbI TGCGCA 1 cut(s) 346
OliI CACNNNNGTG 1 cut(s) 842
PagI TCATGA 1 cut(s) 709
PceI AGGCCT 1 cut(s) 612
PciSI GCTCTTC 1 cut(s) 694
PdiI GCCGGC 1 cut(s) 827
PfeI GAWTC 3 cut(s) 76, 109, 223
PkrI GCNGC 5 cut(s) 301, 304, 717, 861, 963
PleI GAGTC 2 cut(s) 127, 731
PluTI GGCGCC 1 cut(s) 771
PpsI GAGTC 2 cut(s) 127, 731
Ppu21I YACGTR 1 cut(s) 22
PshBI ATTAAT 1 cut(s) 554
Psp6I CCWGG 2 cut(s) 916, 1043
PspFI CCCAGC 1 cut(s) 964
PspGI CCWGG 2 cut(s) 916, 1043
PspN4I GGNNCC 7 cut(s) 396, 744, 769, 948, 1042, 1056, 1057
PspOMI GGGCCC 1 cut(s) 1055
PspPI GGNCC 9 cut(s) 635, 906, 914, 947, 967, 1020, 1041, 1055, 1056
PvuII CAGCTG 1 cut(s) 862
RigI GGCCGGCC 1 cut(s) 829
RsaI GTAC 1 cut(s) 314
RsaNI GTAC 1 cut(s) 313
RseI CAYNNNNRTG 2 cut(s) 507, 842
SapI GCTCTTC 1 cut(s) 694
SaqAI TTAA 1 cut(s) 554
SatI GCNGC 5 cut(s) 300, 303, 716, 860, 962
Sau3AI GATC 2 cut(s) 13, 571
Sau96I GGNCC 9 cut(s) 635, 906, 914, 947, 967, 1020, 1041, 1055, 1056
SchI GAGTC 2 cut(s) 127, 732
ScrFI CCNGG 2 cut(s) 918, 1045
SduI GDGCHC 3 cut(s) 924, 1038, 1059
SetI ASST 9 cut(s) 24, 74, 269, 396, 450, 689, 794, 846, 864
SfaNI GCATC 1 cut(s) 529
SfcI CTRYAG 1 cut(s) 617
SfiI GGCCNNNNNGGCC 1 cut(s) 1054
SfoI GGCGCC 1 cut(s) 769
SinI GGWCC 1 cut(s) 1041
SmiMI CAYNNNNRTG 2 cut(s) 507, 842
Sse9I AATT 4 cut(s) 146, 432, 655, 667
SseBI AGGCCT 1 cut(s) 612
SsiI CCGC 8 cut(s) 143, 158, 283, 299, 302, 471, 715, 798
SspDI GGCGCC 1 cut(s) 767
SspMI CTAG 3 cut(s) 264, 870, 996
StuI AGGCCT 1 cut(s) 612
StyD4I CCNGG 2 cut(s) 916, 1043
StyI CCWWGG 5 cut(s) 84, 390, 502, 869, 882
TaaI ACNGT 2 cut(s) 32, 591
TaiI ACGT 2 cut(s) 24, 450
TaqI TCGA 2 cut(s) 671, 760
TasI AATT 4 cut(s) 146, 432, 655, 667
TauI GCSGC 3 cut(s) 302, 305, 718
TfiI GAWTC 3 cut(s) 76, 109, 223
Tru1I TTAA 1 cut(s) 554
Tru9I TTAA 1 cut(s) 554
TscAI CASTG 5 cut(s) 145, 472, 588, 594, 741
TseFI GTSAC 1 cut(s) 185
TseI GCWGC 2 cut(s) 859, 961
Tsp45I GTSAC 1 cut(s) 185
TspDTI ATGAA 2 cut(s) 139, 645
TspGWI ACGGA 1 cut(s) 854
TspRI CASTG 5 cut(s) 145, 472, 588, 594, 741
VpaK11BI GGWCC 1 cut(s) 1041
VspI ATTAAT 1 cut(s) 554
XagI CCTNNNNNAGG 1 cut(s) 617
XapI RAATTY 2 cut(s) 146, 667
XmaJI CCTAGG 1 cut(s) 869
XspI CTAG 3 cut(s) 264, 870, 996
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.