Rmu_sc0000659.1_g000043

Protein of unknown function (DUF565)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000659.1
OG0012174
Physical Location & Seq
Forward (+)
205469 .. 206977
1509 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 582 bp
atgtgtgaaattcattttgagctccctcagcctttccatttcgttgaggcgttcctgtctggattggcaggcactcttgttagtttcagtagtggagtcgtgttgaatataccaatggcttgttcgtttagtttgccatcaacaggcctccataaatttggattgagtcctcaaagcagagtttcgcacatgaaatatggagttttcactttgagatcctcacctgggtttcttcgtgtccgggctgtgcaagagaatggagggtcccgcagactagtggatataatcagaaccgtgccagagctctcgaggaactactttcgaagtccagctcgtagggctctctttgggggaatatctttgttgggtgggttttatgttgcccaaacaatatctctttcatttggagctttaggagtcaatgatgttattgctgcagttgtgtgtgttctcctgacagaatatgtaaccaaattttattacagcagaccaaaagtaacattccctgttgctcttctcaacaatttcaaaatgggattcacttatggtctgttcatagatgcttttaagcttgccagttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF565

Protein Analysis

193

Amino Acids

21.21

Weight (kDa)

9.84

Isoelectric Point (pI)

59.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF565 PF04483 139 - 193 2.3e-19 Protein of unknown function (DUF565)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012174)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43050
fragaria_vesca FvH4_2g06230
malus_domestica MD05G1079400.v1.1
prunus_persica Prupe.8G123300_v2.0.a1 Prupe.8G123300_v2.0.a1 Prupe.8G123300_v2.0.a1 Prupe.8G123300_v2.0.a1
pyrus_communis pycom05g07250
rosa_chinensis RchiOBHm_Chr6g0256951
rosa_laevigata RLG00000014664
rosa_multiflora Rmu_co8075354.1_g000001 Rmu_sc0000659.1_g000043
rosa_roxburghii Rroxscaffold_7G00208360
rosa_rugosa Rorug05G0576200
rosa_samantha Rh6AG094800 Rh6BG086000 Rh6CG082100 Rh6DG077800
rosa_wichuraiana Rw6G008100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 268
AclWI GGATC 1 cut(s) 210
AcsI RAATTY 3 cut(s) 9, 155, 473
AfiI CCNNNNNNNGG 2 cut(s) 143, 225
AgsI TTSAA 2 cut(s) 106, 529
AhlI ACTAGT 1 cut(s) 274
AjnI CCWGG 1 cut(s) 223
AjuI GAANNNNNNNTTGG 2 cut(s) 106, 138
AluBI AGCT 5 cut(s) 22, 304, 332, 410, 571
AluI AGCT 5 cut(s) 22, 304, 332, 410, 571
Alw21I GWGCWC 2 cut(s) 24, 306
AlwI GGATC 1 cut(s) 210
Ama87I CYCGRG 1 cut(s) 307
AoxI GGCC 1 cut(s) 145
ApeKI GCWGC 1 cut(s) 434
ApoI RAATTY 3 cut(s) 9, 155, 473
AspS9I GGNCC 1 cut(s) 264
AsuC2I CCSGG 1 cut(s) 242
AsuHPI GGTGA 1 cut(s) 213
AsuII TTCGAA 1 cut(s) 322
AvaI CYCGRG 1 cut(s) 307
AvaII GGWCC 1 cut(s) 264
BanII GRGCYC 3 cut(s) 24, 306, 343
Bbv12I GWGCWC 2 cut(s) 24, 306
BbvCI CCTCAGC 1 cut(s) 27
BbvI GCAGC 1 cut(s) 421
BccI CCATC 1 cut(s) 145
BciT130I CCWGG 1 cut(s) 225
BcnI CCSGG 1 cut(s) 242
BcuI ACTAGT 1 cut(s) 274
BfaI CTAG 1 cut(s) 275
BfmI CTRYAG 1 cut(s) 435
BisI GCNGC 1 cut(s) 435
BlsI GCNGC 1 cut(s) 436
Bme1390I CCNGG 2 cut(s) 225, 242
Bme18I GGWCC 1 cut(s) 264
BmeT110I CYCGRG 1 cut(s) 307
BmgT120I GGNCC 1 cut(s) 264
BmiI GGNNCC 2 cut(s) 265, 266
BmrFI CCNGG 2 cut(s) 225, 242
BmsI GCATC 1 cut(s) 550
Bpu10I CCTNAGC 1 cut(s) 27
Bpu14I TTCGAA 1 cut(s) 322
BpuMI CCSGG 1 cut(s) 242
BsaJI CCNNGG 1 cut(s) 224
Bsc4I CCNNNNNNNGG 2 cut(s) 143, 225
Bse1I ACTGG 1 cut(s) 576
BseBI CCWGG 1 cut(s) 225
BseDI CCNNGG 1 cut(s) 224
BseLI CCNNNNNNNGG 2 cut(s) 143, 225
BseMII CTCAG 1 cut(s) 41
BseNI ACTGG 1 cut(s) 576
BseXI GCAGC 1 cut(s) 421
BshFI GGCC 1 cut(s) 147
BsiHKAI GWGCWC 2 cut(s) 24, 306
BsiHKCI CYCGRG 1 cut(s) 307
BsiSI CCGG 1 cut(s) 241
BslFI GGGAC 1 cut(s) 250
BslI CCNNNNNNNGG 2 cut(s) 143, 225
BsmFI GGGAC 1 cut(s) 250
BsnI GGCC 1 cut(s) 147
BsoBI CYCGRG 1 cut(s) 307
Bsp119I TTCGAA 1 cut(s) 322
Bsp1286I GDGCHC 3 cut(s) 24, 306, 343
Bsp143I GATC 1 cut(s) 215
BspACI CCGC 1 cut(s) 268
BspANI GGCC 1 cut(s) 147
BspCNI CTCAG 1 cut(s) 40
BspLI GGNNCC 2 cut(s) 265, 266
BspMAI CTGCAG 1 cut(s) 439
BspPI GGATC 1 cut(s) 210
BspQI GCTCTTC 1 cut(s) 519
BspT104I TTCGAA 1 cut(s) 322
BsrI ACTGG 1 cut(s) 576
BssECI CCNNGG 1 cut(s) 224
BssMI GATC 1 cut(s) 215
Bst2UI CCWGG 1 cut(s) 225
Bst4CI ACNGT 1 cut(s) 295
Bst6I CTCTTC 1 cut(s) 519
BstBI TTCGAA 1 cut(s) 322
BstC8I GCNNGC 2 cut(s) 70, 573
BstDEI CTNAG 1 cut(s) 27
BstKTI GATC 1 cut(s) 218
BstMBI GATC 1 cut(s) 215
BstMWI GCNNNNNNNGC 2 cut(s) 28, 338
BstNI CCWGG 1 cut(s) 225
BstSCI CCNGG 2 cut(s) 223, 240
BstSFI CTRYAG 1 cut(s) 435
BstV1I GCAGC 1 cut(s) 421
BstX2I RGATCY 1 cut(s) 215
BstXI CCANNNNNNTGG 1 cut(s) 158
BstYI RGATCY 1 cut(s) 215
BsuRI GGCC 1 cut(s) 147
Cac8I GCNNGC 2 cut(s) 70, 573
Cfr13I GGNCC 1 cut(s) 264
CviAII CATG 1 cut(s) 190
DdeI CTNAG 1 cut(s) 27
DpnI GATC 1 cut(s) 217
DpnII GATC 1 cut(s) 215
Eam1104I CTCTTC 1 cut(s) 519
EarI CTCTTC 1 cut(s) 519
Ecl136II GAGCTC 2 cut(s) 22, 304
Eco147I AGGCCT 1 cut(s) 147
Eco24I GRGCYC 3 cut(s) 24, 306, 343
Eco47I GGWCC 1 cut(s) 264
Eco53kI GAGCTC 2 cut(s) 22, 304
Eco88I CYCGRG 1 cut(s) 307
EcoICRI GAGCTC 2 cut(s) 22, 304
EcoO109I RGGNCCY 1 cut(s) 264
EcoRII CCWGG 1 cut(s) 223
EcoT38I GRGCYC 3 cut(s) 24, 306, 343
FaeI CATG 1 cut(s) 193
FaiI YATR 9 cut(s) 110, 153, 191, 198, 284, 378, 465, 546, 557
FaqI GGGAC 1 cut(s) 250
FatI CATG 1 cut(s) 189
FauI CCCGC 1 cut(s) 275
Fnu4HI GCNGC 1 cut(s) 435
FriOI GRGCYC 3 cut(s) 24, 306, 343
Fsp4HI GCNGC 1 cut(s) 435
FspBI CTAG 1 cut(s) 275
GluI GCNGC 1 cut(s) 435
HaeIII GGCC 1 cut(s) 147
HapII CCGG 1 cut(s) 241
Hin1II CATG 1 cut(s) 193
HindIII AAGCTT 1 cut(s) 569
HinfI GANTC 4 cut(s) 96, 166, 417, 537
HpaII CCGG 1 cut(s) 241
HphI GGTGA 1 cut(s) 213
Hpy188I TCNGA 1 cut(s) 290
Hpy188III TCNNGA 3 cut(s) 60, 307, 454
HpyCH4III ACNGT 1 cut(s) 295
HpyCH4V TGCA 2 cut(s) 250, 437
HpyF10VI GCNNNNNNNGC 2 cut(s) 28, 338
HpyF3I CTNAG 1 cut(s) 27
Hsp92II CATG 1 cut(s) 193
KflI GGGWCCC 1 cut(s) 264
Kzo9I GATC 1 cut(s) 215
LguI GCTCTTC 1 cut(s) 519
LmnI GCTCC 2 cut(s) 27, 407
Lsp1109I GCAGC 1 cut(s) 421
LweI GCATC 1 cut(s) 550
MaeI CTAG 1 cut(s) 275
MaeIII GTNAC 2 cut(s) 466, 496
MalI GATC 1 cut(s) 217
MboI GATC 1 cut(s) 215
MboII GAAGA 2 cut(s) 224, 506
MflI RGATCY 1 cut(s) 215
MhlI GDGCHC 3 cut(s) 24, 306, 343
MluCI AATT 4 cut(s) 9, 155, 473, 523
MlyI GAGTC 3 cut(s) 105, 175, 426
MnlI CCTC 7 cut(s) 36, 40, 158, 180, 229, 254, 303
MseI TTAA 1 cut(s) 567
MspI CCGG 1 cut(s) 241
MspR9I CCNGG 2 cut(s) 225, 242
MvaI CCWGG 1 cut(s) 225
MwoI GCNNNNNNNGC 2 cut(s) 28, 338
NciI CCSGG 1 cut(s) 242
NdeII GATC 1 cut(s) 215
NlaIII CATG 1 cut(s) 193
NlaIV GGNNCC 2 cut(s) 265, 266
NspV TTCGAA 1 cut(s) 322
PaeR7I CTCGAG 1 cut(s) 307
PceI AGGCCT 1 cut(s) 147
PciSI GCTCTTC 1 cut(s) 519
PfeI GAWTC 1 cut(s) 537
PkrI GCNGC 1 cut(s) 436
PleI GAGTC 3 cut(s) 104, 174, 425
PpsI GAGTC 3 cut(s) 104, 174, 425
PpuMI RGGWCCY 1 cut(s) 264
Psp124BI GAGCTC 2 cut(s) 24, 306
Psp5II RGGWCCY 1 cut(s) 264
Psp6I CCWGG 1 cut(s) 223
PspGI CCWGG 1 cut(s) 223
PspN4I GGNNCC 2 cut(s) 265, 266
PspPI GGNCC 1 cut(s) 264
PspPPI RGGWCCY 1 cut(s) 264
PstI CTGCAG 1 cut(s) 439
PsuI RGATCY 1 cut(s) 215
SacI GAGCTC 2 cut(s) 24, 306
SapI GCTCTTC 1 cut(s) 519
SaqAI TTAA 1 cut(s) 567
SatI GCNGC 1 cut(s) 435
Sau3AI GATC 1 cut(s) 215
Sau96I GGNCC 1 cut(s) 264
SchI GAGTC 3 cut(s) 105, 175, 426
ScrFI CCNGG 2 cut(s) 225, 242
SduI GDGCHC 3 cut(s) 24, 306, 343
SetI ASST 6 cut(s) 24, 226, 306, 334, 412, 573
SfaNI GCATC 1 cut(s) 550
SfcI CTRYAG 1 cut(s) 435
Sfr274I CTCGAG 1 cut(s) 307
SfuI TTCGAA 1 cut(s) 322
SinI GGWCC 1 cut(s) 264
SlaI CTCGAG 1 cut(s) 307
SmlI CTYRAG 1 cut(s) 307
SmoI CTYRAG 1 cut(s) 307
SpeI ACTAGT 1 cut(s) 274
Sse9I AATT 4 cut(s) 9, 155, 473, 523
SseBI AGGCCT 1 cut(s) 147
SsiI CCGC 1 cut(s) 268
SspMI CTAG 1 cut(s) 275
SstI GAGCTC 2 cut(s) 24, 306
StuI AGGCCT 1 cut(s) 147
StyD4I CCNGG 2 cut(s) 223, 240
TaaI ACNGT 1 cut(s) 295
TaqI TCGA 2 cut(s) 308, 322
TasI AATT 4 cut(s) 9, 155, 473, 523
TfiI GAWTC 1 cut(s) 537
Tru1I TTAA 1 cut(s) 567
Tru9I TTAA 1 cut(s) 567
TseI GCWGC 1 cut(s) 434
TspDTI ATGAA 3 cut(s) 206, 390, 544
VpaK11BI GGWCC 1 cut(s) 264
XapI RAATTY 3 cut(s) 9, 155, 473
XhoI CTCGAG 1 cut(s) 307
XspI CTAG 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.