Rmu_sc0000756.1_g000011

Pleckstrin homology domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000756.1
OG0018784
Physical Location & Seq
Forward (+)
61251 .. 62178
928 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 411 bp
atgtttcttgattccggcgacgaggcagtagccgtacgagcggccgcgcgtgccttcgtcgaaggccttgacgatgatccccttgcagccgccgtagtgaccttggaggaggatggagaggagattcagactgagctgaccggtgcgaagaaggactccgccgtcacgcgcgcgtcgaggacacgcggcgtgatcccgttggccacgtgcctggtgaagggcgcggaggacgtgctgaacaagcagtacgcgttcgagctctccacgcgctccgagaccatgtacttcatcgccgactccgagaaggagaaggaggactggatcaactcgatcggacggtcgatcgtgcagcactccaggtccgtcacagactccgaggtcgtcgattacgatagcaacaacaagaactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005543 GO:0008289 GO:0032266 GO:0035091 GO:0043167 GO:0043168 GO:1901981
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PH

Protein Analysis

136

Amino Acids

14.86

Weight (kDa)

4.43

Isoelectric Point (pI)

48.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH PF00169 54 - 115 4.7e-08 PH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018784)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22321 FvH4_3g30191 FvH4_3g31746 FvH4_3g31746
pyrus_communis pycom09g05880
rosa_chinensis RchiOBHm_Chr7g0220901
rosa_multiflora Rmu_sc0000756.1_g000011
rosa_roxburghii Rroxscaffold_2G00094810
rosa_samantha Rh5BG308800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 161, 377
AccBSI CCGCTC 1 cut(s) 41
AccII CGCG 9 cut(s) 47, 49, 169, 171, 173, 186, 224, 251, 268
AciI CCGC 6 cut(s) 41, 45, 90, 159, 186, 224
AclWI GGATC 3 cut(s) 71, 187, 329
AcoI YGGCCR 2 cut(s) 42, 201
AcvI CACGTG 1 cut(s) 207
AfaI GTAC 3 cut(s) 36, 248, 284
AfiI CCNNNNNNNGG 1 cut(s) 217
AflIII ACRYGT 1 cut(s) 249
AgeI ACCGGT 1 cut(s) 140
AjiI CACGTC 1 cut(s) 232
AjnI CCWGG 2 cut(s) 210, 356
AluBI AGCT 2 cut(s) 136, 259
AluI AGCT 2 cut(s) 136, 259
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 1 cut(s) 269
AlwI GGATC 3 cut(s) 71, 187, 329
AoxI GGCC 3 cut(s) 42, 64, 201
ApeKI GCWGC 2 cut(s) 86, 349
AsiGI ACCGGT 1 cut(s) 140
AspLEI GCGC 5 cut(s) 49, 171, 173, 224, 270
AspS9I GGNCC 1 cut(s) 360
AsuHPI GGTGA 1 cut(s) 226
AvaII GGWCC 1 cut(s) 360
BalI TGGCCA 1 cut(s) 203
BanII GRGCYC 1 cut(s) 261
BbrPI CACGTG 1 cut(s) 207
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 2 cut(s) 98, 361
BccI CCATC 1 cut(s) 107
BceAI ACGGC 3 cut(s) 17, 77, 146
BciT130I CCWGG 2 cut(s) 212, 358
BcoDI GTCTC 1 cut(s) 269
BisI GCNGC 6 cut(s) 42, 45, 87, 90, 187, 350
BlsI GCNGC 6 cut(s) 43, 46, 88, 91, 188, 351
Bme1390I CCNGG 2 cut(s) 212, 358
Bme18I GGWCC 1 cut(s) 360
BmgBI CACGTC 1 cut(s) 232
BmgT120I GGNCC 1 cut(s) 360
BmrFI CCNGG 2 cut(s) 212, 358
BpmI CTGGAG 1 cut(s) 340
BsaAI YACGTR 1 cut(s) 207
BsaI GGTCTC 1 cut(s) 269
BsaJI CCNNGG 2 cut(s) 102, 375
BsaWI WCCGGW 1 cut(s) 140
Bsc4I CCNNNNNNNGG 1 cut(s) 217
Bse118I RCCGGY 1 cut(s) 140
Bse1I ACTGG 1 cut(s) 323
BseBI CCWGG 2 cut(s) 212, 358
BseDI CCNNGG 2 cut(s) 102, 375
BseGI GGATG 1 cut(s) 118
BseLI CCNNNNNNNGG 1 cut(s) 217
BseMII CTCAG 1 cut(s) 123
BseNI ACTGG 1 cut(s) 323
BsePI GCGCGC 1 cut(s) 169
BseRI GAGGAG 2 cut(s) 122, 134
BseX3I CGGCCG 1 cut(s) 42
BseXI GCAGC 2 cut(s) 98, 361
BsgI GTGCAG 1 cut(s) 368
Bsh1236I CGCG 9 cut(s) 47, 49, 169, 171, 173, 186, 224, 251, 268
Bsh1285I CGRYCG 4 cut(s) 45, 333, 341, 345
BshFI GGCC 3 cut(s) 44, 66, 203
BshTI ACCGGT 1 cut(s) 140
BsiEI CGRYCG 4 cut(s) 45, 333, 341, 345
BsiHKAI GWGCWC 1 cut(s) 261
BsiSI CCGG 2 cut(s) 15, 141
BsiWI CGTACG 1 cut(s) 34
BslI CCNNNNNNNGG 1 cut(s) 217
BsmAI GTCTC 1 cut(s) 269
BsnI GGCC 3 cut(s) 44, 66, 203
Bso31I GGTCTC 1 cut(s) 269
Bsp1286I GDGCHC 1 cut(s) 261
Bsp143I GATC 5 cut(s) 76, 192, 321, 330, 342
BspACI CCGC 6 cut(s) 41, 45, 90, 159, 186, 224
BspANI GGCC 3 cut(s) 44, 66, 203
BspCNI CTCAG 1 cut(s) 124
BspFNI CGCG 9 cut(s) 47, 49, 169, 171, 173, 186, 224, 251, 268
BspPI GGATC 3 cut(s) 71, 187, 329
BspTNI GGTCTC 1 cut(s) 269
BsrBI CCGCTC 1 cut(s) 41
BsrFI RCCGGY 1 cut(s) 140
BsrI ACTGG 1 cut(s) 323
BssAI RCCGGY 1 cut(s) 140
BssECI CCNNGG 2 cut(s) 102, 375
BssHII GCGCGC 1 cut(s) 169
BssMI GATC 5 cut(s) 76, 192, 321, 330, 342
BssT1I CCWWGG 1 cut(s) 102
Bst2UI CCWGG 2 cut(s) 212, 358
Bst4CI ACNGT 1 cut(s) 339
BstBAI YACGTR 1 cut(s) 207
BstC8I GCNNGC 2 cut(s) 51, 171
BstDEI CTNAG 1 cut(s) 132
BstENI CCTNNNNNAGG 1 cut(s) 215
BstF5I GGATG 1 cut(s) 118
BstFNI CGCG 9 cut(s) 47, 49, 169, 171, 173, 186, 224, 251, 268
BstHHI GCGC 5 cut(s) 49, 171, 173, 224, 270
BstKTI GATC 5 cut(s) 79, 195, 324, 333, 345
BstMAI GTCTC 1 cut(s) 269
BstMBI GATC 5 cut(s) 76, 192, 321, 330, 342
BstMCI CGRYCG 4 cut(s) 45, 333, 341, 345
BstMWI GCNNNNNNNGC 4 cut(s) 38, 50, 241, 265
BstNI CCWGG 2 cut(s) 212, 358
BstSCI CCNGG 2 cut(s) 210, 356
BstUI CGCG 9 cut(s) 47, 49, 169, 171, 173, 186, 224, 251, 268
BstV1I GCAGC 2 cut(s) 98, 361
BstXI CCANNNNNNTGG 1 cut(s) 211
BstZI CGGCCG 1 cut(s) 42
BsuRI GGCC 3 cut(s) 44, 66, 203
BtgZI GCGATG 1 cut(s) 274
BtrI CACGTC 1 cut(s) 232
BtsCI GGATG 1 cut(s) 118
Cac8I GCNNGC 2 cut(s) 51, 171
CciNI GCGGCCGC 1 cut(s) 42
CfoI GCGC 5 cut(s) 49, 171, 173, 224, 270
Cfr10I RCCGGY 1 cut(s) 140
Cfr13I GGNCC 1 cut(s) 360
CseI GACGC 1 cut(s) 162
Csp6I GTAC 3 cut(s) 35, 247, 283
CspAI ACCGGT 1 cut(s) 140
CviAII CATG 1 cut(s) 280
CviJI RGCY 7 cut(s) 32, 44, 66, 89, 136, 203, 259
CviKI_1 RGCY 7 cut(s) 32, 44, 66, 89, 136, 203, 259
CviQI GTAC 3 cut(s) 35, 247, 283
DdeI CTNAG 1 cut(s) 132
DpnI GATC 5 cut(s) 78, 194, 323, 332, 344
DpnII GATC 5 cut(s) 76, 192, 321, 330, 342
DrdI GACNNNNNNGTC 2 cut(s) 161, 377
DseDI GACNNNNNNGTC 2 cut(s) 161, 377
EaeI YGGCCR 2 cut(s) 42, 201
EagI CGGCCG 1 cut(s) 42
EciI GGCGGA 1 cut(s) 148
Ecl136II GAGCTC 1 cut(s) 259
EclXI CGGCCG 1 cut(s) 42
Eco130I CCWWGG 1 cut(s) 102
Eco147I AGGCCT 1 cut(s) 66
Eco24I GRGCYC 1 cut(s) 261
Eco31I GGTCTC 1 cut(s) 269
Eco47I GGWCC 1 cut(s) 360
Eco52I CGGCCG 1 cut(s) 42
Eco53kI GAGCTC 1 cut(s) 259
Eco72I CACGTG 1 cut(s) 207
EcoICRI GAGCTC 1 cut(s) 259
EcoNI CCTNNNNNAGG 1 cut(s) 215
EcoRII CCWGG 2 cut(s) 210, 356
EcoT14I CCWWGG 1 cut(s) 102
EcoT38I GRGCYC 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 102
FaeI CATG 1 cut(s) 283
FaiI YATR 1 cut(s) 281
FatI CATG 1 cut(s) 279
Fnu4HI GCNGC 6 cut(s) 42, 45, 87, 90, 187, 350
FokI GGATG 1 cut(s) 125
FriOI GRGCYC 1 cut(s) 261
Fsp4HI GCNGC 6 cut(s) 42, 45, 87, 90, 187, 350
GlaI GCGC 5 cut(s) 48, 170, 172, 223, 269
GluI GCNGC 6 cut(s) 42, 45, 87, 90, 187, 350
GsuI CTGGAG 1 cut(s) 340
HaeIII GGCC 3 cut(s) 44, 66, 203
HapII CCGG 2 cut(s) 15, 141
HgaI GACGC 1 cut(s) 162
HhaI GCGC 5 cut(s) 49, 171, 173, 224, 270
Hin1II CATG 1 cut(s) 283
Hin6I GCGC 5 cut(s) 47, 169, 171, 222, 268
HinP1I GCGC 5 cut(s) 47, 169, 171, 222, 268
HinfI GANTC 5 cut(s) 11, 124, 155, 296, 371
HpaII CCGG 2 cut(s) 15, 141
HphI GGTGA 1 cut(s) 226
Hpy188I TCNGA 5 cut(s) 129, 274, 301, 335, 376
Hpy188III TCNNGA 1 cut(s) 8
Hpy99I CGWCG 4 cut(s) 23, 62, 178, 386
HpyAV CCTTC 6 cut(s) 56, 64, 145, 211, 298, 304
HpyCH4III ACNGT 1 cut(s) 339
HpyCH4IV ACGT 2 cut(s) 206, 231
HpyCH4V TGCA 2 cut(s) 86, 349
HpyF10VI GCNNNNNNNGC 4 cut(s) 38, 50, 241, 265
HpyF3I CTNAG 1 cut(s) 132
HpySE526I ACGT 2 cut(s) 206, 231
Hsp92II CATG 1 cut(s) 283
HspAI GCGC 5 cut(s) 47, 169, 171, 222, 268
Kzo9I GATC 5 cut(s) 76, 192, 321, 330, 342
LmnI GCTCC 1 cut(s) 275
LpnPI CCDG 7 cut(s) 28, 154, 197, 224, 304, 343, 370
Lsp1109I GCAGC 2 cut(s) 98, 361
MaeII ACGT 2 cut(s) 206, 231
MaeIII GTNAC 3 cut(s) 97, 163, 364
MalI GATC 5 cut(s) 78, 194, 323, 332, 344
MauBI CGCGCGCG 1 cut(s) 169
MbiI CCGCTC 1 cut(s) 41
MboI GATC 5 cut(s) 76, 192, 321, 330, 342
MboII GAAGA 1 cut(s) 160
MhlI GDGCHC 1 cut(s) 261
MlsI TGGCCA 1 cut(s) 203
MluI ACGCGT 1 cut(s) 249
MluNI TGGCCA 1 cut(s) 203
MlyI GAGTC 3 cut(s) 149, 290, 365
MnlI CCTC 8 cut(s) 16, 100, 103, 112, 171, 220, 307, 370
Mox20I TGGCCA 1 cut(s) 203
MscI TGGCCA 1 cut(s) 203
Msp20I TGGCCA 1 cut(s) 203
MspI CCGG 2 cut(s) 15, 141
MspR9I CCNGG 2 cut(s) 212, 358
MvaI CCWGG 2 cut(s) 212, 358
MvnI CGCG 9 cut(s) 47, 49, 169, 171, 173, 186, 224, 251, 268
MwoI GCNNNNNNNGC 4 cut(s) 38, 50, 241, 265
NdeII GATC 5 cut(s) 76, 192, 321, 330, 342
NlaIII CATG 1 cut(s) 283
NmuCI GTSAC 3 cut(s) 97, 163, 364
NotI GCGGCCGC 1 cut(s) 42
PauI GCGCGC 1 cut(s) 169
PceI AGGCCT 1 cut(s) 66
PcsI WCGNNNNNNNCGW 3 cut(s) 173, 297, 387
PfeI GAWTC 2 cut(s) 11, 124
Pfl23II CGTACG 1 cut(s) 34
PinAI ACCGGT 1 cut(s) 140
PkrI GCNGC 6 cut(s) 43, 46, 88, 91, 188, 351
Ple19I CGATCG 2 cut(s) 333, 345
PleI GAGTC 3 cut(s) 149, 290, 365
PmaCI CACGTG 1 cut(s) 207
PmlI CACGTG 1 cut(s) 207
PpsI GAGTC 3 cut(s) 149, 290, 365
Ppu21I YACGTR 1 cut(s) 207
Psp124BI GAGCTC 1 cut(s) 261
Psp6I CCWGG 2 cut(s) 210, 356
PspCI CACGTG 1 cut(s) 207
PspGI CCWGG 2 cut(s) 210, 356
PspLI CGTACG 1 cut(s) 34
PspPI GGNCC 1 cut(s) 360
PsrI GAACNNNNNNTAC 2 cut(s) 230, 262
PteI GCGCGC 1 cut(s) 169
PvuI CGATCG 2 cut(s) 333, 345
RsaI GTAC 3 cut(s) 36, 248, 284
RsaNI GTAC 3 cut(s) 35, 247, 283
SacI GAGCTC 1 cut(s) 261
SatI GCNGC 6 cut(s) 42, 45, 87, 90, 187, 350
Sau3AI GATC 5 cut(s) 76, 192, 321, 330, 342
Sau96I GGNCC 1 cut(s) 360
SchI GAGTC 3 cut(s) 149, 290, 365
ScrFI CCNGG 2 cut(s) 212, 358
SduI GDGCHC 1 cut(s) 261
SetI ASST 7 cut(s) 104, 138, 209, 234, 261, 362, 381
SinI GGWCC 1 cut(s) 360
SseBI AGGCCT 1 cut(s) 66
SsiI CCGC 6 cut(s) 41, 45, 90, 159, 186, 224
SstI GAGCTC 1 cut(s) 261
StuI AGGCCT 1 cut(s) 66
StyD4I CCNGG 2 cut(s) 210, 356
StyI CCWWGG 1 cut(s) 102
TaaI ACNGT 1 cut(s) 339
TaiI ACGT 2 cut(s) 209, 234
TaqI TCGA 6 cut(s) 60, 176, 255, 329, 341, 384
TatI WGTACW 1 cut(s) 282
TauI GCSGC 4 cut(s) 44, 47, 92, 189
TfiI GAWTC 2 cut(s) 11, 124
TseFI GTSAC 3 cut(s) 97, 163, 364
TseI GCWGC 2 cut(s) 86, 349
Tsp45I GTSAC 3 cut(s) 97, 163, 364
TspDTI ATGAA 1 cut(s) 277
TspGWI ACGGA 1 cut(s) 352
VpaK11BI GGWCC 1 cut(s) 360
XagI CCTNNNNNAGG 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.