Rmu_sc0001250.1_g000040

Putative lipopolysaccharide-modifying enzyme.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001250.1
OG0008951
Physical Location & Seq
Forward (+)
194437 .. 196937
2501 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1215 bp
atgttgcgcgggtctcgttttgtcaaaccttttcagcttcaggctgcaacaaattggcaatcctctttgataaagggacctccagctgatactatcttcttcttgggttttctaatcattgcagccgttctatcttctttctggattcacctttggacttttcagattgatcattctacaaaccaaacaatagtgatcaacgacaagtatgagataacccccaaaataatcgagtttccactcaactgttctatcggcatcaatcaaacacaaacgtgtccaacaaattaccctatagcctttactagtgatcgtcttgacccatcgtcaaacggtacatgcccggactatttccggtttatccacgaagatctaaagccatggagaaccacaggaatcacaagggacatggtggagaggggaaagaagacggcacattttcggctagtgattgttaaaggcaaggcttatattgacaggtacaagaagtccatacagacaagggatatgtttaccatatggggtattttgcagcttcttaggaggtaccccgggaaagtacctgacttggagctgatgtttgactgtgatgaccttccggttatccgatcacgggactaccggggacagaactcgacgcaggtgccaccgctgttccggtactgtggtgatagatggacaaaagacattgtgttccctgattggtctttctggggttgggctgagataaacataaaaccgtggggaagtttgttgaaagatgtgaagaaaggtaacgagaggagcaaatggatggaaagagaaccttatgcatactggaaagggaacccctttgtagctgaatcaaggagagacctcctcaaatgcaacgtctcggatacacaggactggaatgctcgcttattcatccaggactggatccttgaatctcaacaaggcttcaagcattcagatgtaacaaaccaatgcacacatagacttcagaacttcagatcggtggtggaggaatctcccggtctccagtcttcagtctccagtctcactctcccggtctccagccaccgagctacctcctcggctcctccgccaccgtccactgagccacatccaaggctgacggctccactgggacctgcggactgcggtctgcatctctggattccctgcgtcatgcgactcagccgactcctgcaatcctgctccttgaactcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
Glyco_transf_90

Protein Analysis

404

Amino Acids

46.59

Weight (kDa)

9.1

Isoelectric Point (pI)

41.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_90 PF05686 110 - 328 2.6e-104 Glycosyl transferase family 90
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 631
Acc36I ACCTGC 2 cut(s) 631, 1141
Acc65I GGTACC 1 cut(s) 546
AccB1I GGYRCC 2 cut(s) 546, 643
AccII CGCG 1 cut(s) 9
AciI CCGC 5 cut(s) 9, 650, 1085, 1136, 1143
AclWI GGATC 2 cut(s) 913, 926
AcuI CTGAAG 4 cut(s) 23, 965, 973, 1011
AfaI GTAC 5 cut(s) 337, 482, 548, 561, 662
AfiI CCNNNNNNNGG 1 cut(s) 613
AflIII ACRYGT 1 cut(s) 275
AgsI TTSAA 4 cut(s) 757, 926, 943, 1207
AhdI GACNNNNNGTC 1 cut(s) 325
AhlI ACTAGT 1 cut(s) 305
AjnI CCWGG 1 cut(s) 909
AleI CACNNNNGTG 1 cut(s) 274
AluBI AGCT 6 cut(s) 37, 86, 535, 574, 839, 1067
AluI AGCT 6 cut(s) 37, 86, 535, 574, 839, 1067
Alw26I GTCTC 7 cut(s) 18, 846, 877, 1022, 1036, 1043, 1057
AlwI GGATC 2 cut(s) 913, 926
Ama87I CYCGRG 1 cut(s) 551
ApeKI GCWGC 3 cut(s) 44, 122, 532
ArsI GACNNNNNNTTYG 2 cut(s) 855, 887
Asp718I GGTACC 1 cut(s) 546
AspLEI GCGC 1 cut(s) 9
AspS9I GGNCC 2 cut(s) 77, 1130
AsuC2I CCSGG 6 cut(s) 344, 552, 553, 623, 1014, 1049
AsuHPI GGTGA 2 cut(s) 140, 680
AvaI CYCGRG 1 cut(s) 551
AvaII GGWCC 2 cut(s) 77, 1130
BaeI ACNNNNGTAYC 2 cut(s) 652, 685
BamHI GGATCC 1 cut(s) 918
BanI GGYRCC 2 cut(s) 546, 643
BbsI GAAGAC 2 cut(s) 434, 1017
BbvI GCAGC 3 cut(s) 31, 134, 544
BccI CCATC 3 cut(s) 331, 669, 787
BceAI ACGGC 3 cut(s) 110, 447, 1134
BcgI CGANNNNNNTGC 2 cut(s) 625, 659
BciT130I CCWGG 1 cut(s) 911
BciVI GTATCC 1 cut(s) 871
BclI TGATCA 2 cut(s) 169, 195
BcnI CCSGG 6 cut(s) 344, 552, 553, 623, 1014, 1049
BcoDI GTCTC 7 cut(s) 18, 846, 877, 1022, 1036, 1043, 1057
BcuI ACTAGT 1 cut(s) 305
BfaI CTAG 2 cut(s) 306, 446
BfmI CTRYAG 1 cut(s) 294
BfuAI ACCTGC 2 cut(s) 631, 1141
BfuI GTATCC 1 cut(s) 871
BglII AGATCT 1 cut(s) 370
BisI GCNGC 3 cut(s) 45, 123, 533
BlsI GCNGC 3 cut(s) 46, 124, 534
Bme1390I CCNGG 7 cut(s) 344, 552, 553, 623, 911, 1014, 1049
Bme18I GGWCC 2 cut(s) 77, 1130
BmeRI GACNNNNNGTC 1 cut(s) 325
BmeT110I CYCGRG 1 cut(s) 551
BmgT120I GGNCC 2 cut(s) 77, 1130
BmiI GGNNCC 8 cut(s) 78, 548, 645, 827, 920, 1080, 1122, 1131
BmrFI CCNGG 7 cut(s) 344, 552, 553, 623, 911, 1014, 1049
BmrI ACTGGG 1 cut(s) 1136
BmsI GCATC 2 cut(s) 267, 1159
BmuI ACTGGG 1 cut(s) 1136
BoxI GACNNNNGTC 1 cut(s) 1143
BpiI GAAGAC 2 cut(s) 434, 1017
BplI GAGNNNNNCTC 2 cut(s) 843, 875
BpmI CTGGAG 4 cut(s) 66, 1004, 1018, 1039
BpuMI CCSGG 6 cut(s) 344, 552, 553, 623, 1014, 1049
BsaI GGTCTC 4 cut(s) 18, 846, 1022, 1057
BsaJI CCNNGG 7 cut(s) 380, 550, 551, 622, 740, 1074, 1109
BsaWI WCCGGW 3 cut(s) 354, 598, 657
BsaXI ACNNNNNCTCC 2 cut(s) 563, 593
Bsc4I CCNNNNNNNGG 1 cut(s) 613
Bse1I ACTGG 6 cut(s) 821, 893, 920, 1021, 1035, 1131
Bse3DI GCAATG 1 cut(s) 117
BseBI CCWGG 1 cut(s) 911
BseDI CCNNGG 7 cut(s) 380, 550, 551, 622, 740, 1074, 1109
BseGI GGATG 3 cut(s) 798, 906, 1105
BseLI CCNNNNNNNGG 1 cut(s) 613
BseMI GCAATG 1 cut(s) 117
BseMII CTCAG 3 cut(s) 714, 1089, 1192
BseNI ACTGG 6 cut(s) 821, 893, 920, 1021, 1035, 1131
BseRI GAGGAG 4 cut(s) 796, 848, 1063, 1071
BseXI GCAGC 3 cut(s) 31, 134, 544
Bsh1236I CGCG 1 cut(s) 9
BshNI GGYRCC 2 cut(s) 546, 643
BsiHKCI CYCGRG 1 cut(s) 551
BsiSI CCGG 8 cut(s) 344, 355, 552, 599, 622, 658, 1014, 1049
BslFI GGGAC 5 cut(s) 90, 419, 629, 639, 1143
BslI CCNNNNNNNGG 1 cut(s) 613
BsmAI GTCTC 7 cut(s) 18, 846, 877, 1022, 1036, 1043, 1057
BsmBI CGTCTC 1 cut(s) 877
BsmFI GGGAC 5 cut(s) 90, 419, 629, 639, 1143
BsmI GAATGC 2 cut(s) 898, 946
Bso31I GGTCTC 4 cut(s) 18, 846, 1022, 1057
BsoBI CYCGRG 1 cut(s) 551
Bsp143I GATC 7 cut(s) 169, 195, 310, 370, 608, 918, 992
Bsp19I CCATGG 1 cut(s) 380
BspACI CCGC 5 cut(s) 9, 650, 1085, 1136, 1143
BspCNI CTCAG 3 cut(s) 715, 1090, 1191
BspFNI CGCG 1 cut(s) 9
BspLI GGNNCC 8 cut(s) 78, 548, 645, 827, 920, 1080, 1122, 1131
BspMI ACCTGC 2 cut(s) 631, 1141
BspPI GGATC 2 cut(s) 913, 926
BspT107I GGYRCC 2 cut(s) 546, 643
BspTNI GGTCTC 4 cut(s) 18, 846, 1022, 1057
BsrDI GCAATG 1 cut(s) 117
BsrI ACTGG 6 cut(s) 821, 893, 920, 1021, 1035, 1131
BssECI CCNNGG 7 cut(s) 380, 550, 551, 622, 740, 1074, 1109
BssMI GATC 7 cut(s) 169, 195, 310, 370, 608, 918, 992
BssT1I CCWWGG 2 cut(s) 380, 1109
Bst2UI CCWGG 1 cut(s) 911
Bst4CI ACNGT 6 cut(s) 248, 335, 587, 665, 741, 1092
BstC8I GCNNGC 1 cut(s) 898
BstDEI CTNAG 4 cut(s) 539, 723, 1098, 1178
BstDSI CCRYGG 2 cut(s) 380, 740
BstF5I GGATG 3 cut(s) 798, 906, 1105
BstFNI CGCG 1 cut(s) 9
BstHHI GCGC 1 cut(s) 9
BstKTI GATC 7 cut(s) 172, 198, 313, 373, 611, 921, 995
BstMAI GTCTC 7 cut(s) 18, 846, 877, 1022, 1036, 1043, 1057
BstMBI GATC 7 cut(s) 169, 195, 310, 370, 608, 918, 992
BstNI CCWGG 1 cut(s) 911
BstNSI RCATGY 1 cut(s) 342
BstPAI GACNNNNGTC 1 cut(s) 1143
BstSCI CCNGG 7 cut(s) 342, 550, 551, 621, 909, 1012, 1047
BstSFI CTRYAG 1 cut(s) 294
BstUI CGCG 1 cut(s) 9
BstV1I GCAGC 3 cut(s) 31, 134, 544
BstV2I GAAGAC 2 cut(s) 434, 1017
BstX2I RGATCY 2 cut(s) 370, 918
BstYI RGATCY 2 cut(s) 370, 918
BsuI GTATCC 1 cut(s) 871
BtgI CCRYGG 2 cut(s) 380, 740
BtsCI GGATG 3 cut(s) 798, 906, 1105
BtsIMutI CAGTG 2 cut(s) 1095, 1124
BveI ACCTGC 2 cut(s) 631, 1141
Cac8I GCNNGC 1 cut(s) 898
CfoI GCGC 1 cut(s) 9
Cfr13I GGNCC 2 cut(s) 77, 1130
Cfr9I CCCGGG 1 cut(s) 551
CseI GACGC 2 cut(s) 646, 1156
Csp6I GTAC 5 cut(s) 336, 481, 547, 560, 661
CviAII CATG 4 cut(s) 339, 381, 409, 1171
CviQI GTAC 5 cut(s) 336, 481, 547, 560, 661
DdeI CTNAG 4 cut(s) 539, 723, 1098, 1178
DpnI GATC 7 cut(s) 171, 197, 312, 372, 610, 920, 994
DpnII GATC 7 cut(s) 169, 195, 310, 370, 608, 918, 992
DriI GACNNNNNGTC 1 cut(s) 325
Eam1105I GACNNNNNGTC 1 cut(s) 325
EciI GGCGGA 1 cut(s) 1074
Eco130I CCWWGG 2 cut(s) 380, 1109
Eco31I GGTCTC 4 cut(s) 18, 846, 1022, 1057
Eco47I GGWCC 2 cut(s) 77, 1130
Eco57I CTGAAG 4 cut(s) 23, 965, 973, 1011
Eco88I CYCGRG 1 cut(s) 551
EcoO109I RGGNCCY 2 cut(s) 77, 1130
EcoRII CCWGG 1 cut(s) 909
EcoT14I CCWWGG 2 cut(s) 380, 1109
EcoT22I ATGCAT 1 cut(s) 814
ErhI CCWWGG 2 cut(s) 380, 1109
Esp3I CGTCTC 1 cut(s) 877
FaeI CATG 4 cut(s) 342, 384, 412, 1174
FalI AAGNNNNNCTT 2 cut(s) 790, 822
FaqI GGGAC 5 cut(s) 90, 419, 629, 639, 1143
FatI CATG 4 cut(s) 338, 380, 408, 1170
FauI CCCGC 1 cut(s) 2
FauNDI CATATG 1 cut(s) 518
FbaI TGATCA 2 cut(s) 169, 195
Fnu4HI GCNGC 3 cut(s) 45, 123, 533
FokI GGATG 3 cut(s) 805, 893, 1092
Fsp4HI GCNGC 3 cut(s) 45, 123, 533
FspBI CTAG 2 cut(s) 306, 446
GlaI GCGC 1 cut(s) 8
GluI GCNGC 3 cut(s) 45, 123, 533
GsuI CTGGAG 4 cut(s) 66, 1004, 1018, 1039
HapII CCGG 8 cut(s) 344, 355, 552, 599, 622, 658, 1014, 1049
HgaI GACGC 2 cut(s) 646, 1156
HhaI GCGC 1 cut(s) 9
Hin1II CATG 4 cut(s) 342, 384, 412, 1174
Hin6I GCGC 1 cut(s) 7
HinP1I GCGC 1 cut(s) 7
HinfI GANTC 8 cut(s) 145, 396, 842, 926, 1007, 1159, 1176, 1185
HpaII CCGG 8 cut(s) 344, 355, 552, 599, 622, 658, 1014, 1049
HphI GGTGA 2 cut(s) 140, 680
Hpy166II GTNNAC 2 cut(s) 513, 1095
Hpy188I TCNGA 6 cut(s) 165, 608, 877, 952, 984, 992
Hpy188III TCNNGA 4 cut(s) 142, 317, 1156, 1212
Hpy8I GTNNAC 2 cut(s) 513, 1095
Hpy99I CGWCG 1 cut(s) 640
HpyAV CCTTC 1 cut(s) 605
HpyCH4III ACNGT 6 cut(s) 248, 335, 587, 665, 741, 1092
HpyCH4IV ACGT 2 cut(s) 275, 870
HpyCH4V TGCA 8 cut(s) 47, 122, 532, 812, 867, 969, 1150, 1192
HpyF3I CTNAG 4 cut(s) 539, 723, 1098, 1178
HpySE526I ACGT 2 cut(s) 275, 870
Hsp92II CATG 4 cut(s) 342, 384, 412, 1174
HspAI GCGC 1 cut(s) 7
KpnI GGTACC 1 cut(s) 550
Ksp22I TGATCA 2 cut(s) 169, 195
Kzo9I GATC 7 cut(s) 169, 195, 310, 370, 608, 918, 992
LmnI GCTCC 5 cut(s) 571, 783, 1084, 1126, 1205
Lsp1109I GCAGC 3 cut(s) 31, 134, 544
LweI GCATC 2 cut(s) 267, 1159
MaeI CTAG 2 cut(s) 306, 446
MaeII ACGT 2 cut(s) 275, 870
MaeIII GTNAC 2 cut(s) 773, 955
MalI GATC 7 cut(s) 171, 197, 312, 372, 610, 920, 994
MboI GATC 7 cut(s) 169, 195, 310, 370, 608, 918, 992
MboII GAAGA 7 cut(s) 88, 91, 126, 380, 439, 778, 1017
MflI RGATCY 2 cut(s) 370, 918
MluCI AATT 2 cut(s) 52, 286
MlyI GAGTC 2 cut(s) 1170, 1179
MmeI TCCRAC 1 cut(s) 305
Mph1103I ATGCAT 1 cut(s) 814
MseI TTAA 1 cut(s) 456
MslI CAYNNNNRTG 2 cut(s) 274, 951
MspA1I CMGCKG 2 cut(s) 86, 652
MspI CCGG 8 cut(s) 344, 355, 552, 599, 622, 658, 1014, 1049
MspR9I CCNGG 7 cut(s) 344, 552, 553, 623, 911, 1014, 1049
Mva1269I GAATGC 2 cut(s) 898, 946
MvaI CCWGG 1 cut(s) 911
MvnI CGCG 1 cut(s) 9
NciI CCSGG 6 cut(s) 344, 552, 553, 623, 1014, 1049
NcoI CCATGG 1 cut(s) 380
NdeI CATATG 1 cut(s) 518
NdeII GATC 7 cut(s) 169, 195, 310, 370, 608, 918, 992
NlaIII CATG 4 cut(s) 342, 384, 412, 1174
NlaIV GGNNCC 8 cut(s) 78, 548, 645, 827, 920, 1080, 1122, 1131
NmeAIII GCCGAG 1 cut(s) 1055
NsiI ATGCAT 1 cut(s) 814
NspI RCATGY 1 cut(s) 342
OliI CACNNNNGTG 1 cut(s) 274
PaqCI CACCTGC 1 cut(s) 631
PctI GAATGC 2 cut(s) 898, 946
PfeI GAWTC 6 cut(s) 145, 396, 842, 926, 1007, 1159
PfoI TCCNGGA 1 cut(s) 909
PkrI GCNGC 3 cut(s) 46, 124, 534
PleI GAGTC 2 cut(s) 1170, 1179
PpsI GAGTC 2 cut(s) 1170, 1179
PpuMI RGGWCCY 2 cut(s) 77, 1130
PshAI GACNNNNGTC 1 cut(s) 1143
Psp5II RGGWCCY 2 cut(s) 77, 1130
Psp6I CCWGG 1 cut(s) 909
PspGI CCWGG 1 cut(s) 909
PspN4I GGNNCC 8 cut(s) 78, 548, 645, 827, 920, 1080, 1122, 1131
PspPI GGNCC 2 cut(s) 77, 1130
PspPPI RGGWCCY 2 cut(s) 77, 1130
PsuI RGATCY 2 cut(s) 370, 918
PvuII CAGCTG 1 cut(s) 86
RsaI GTAC 5 cut(s) 337, 482, 548, 561, 662
RsaNI GTAC 5 cut(s) 336, 481, 547, 560, 661
RseI CAYNNNNRTG 2 cut(s) 274, 951
SaqAI TTAA 1 cut(s) 456
SatI GCNGC 3 cut(s) 45, 123, 533
Sau3AI GATC 7 cut(s) 169, 195, 310, 370, 608, 918, 992
Sau96I GGNCC 2 cut(s) 77, 1130
SchI GAGTC 2 cut(s) 1170, 1179
ScrFI CCNGG 7 cut(s) 344, 552, 553, 623, 911, 1014, 1049
SfaNI GCATC 2 cut(s) 267, 1159
SfcI CTRYAG 1 cut(s) 294
SinI GGWCC 2 cut(s) 77, 1130
SmaI CCCGGG 1 cut(s) 553
SmiMI CAYNNNNRTG 2 cut(s) 274, 951
SpeI ACTAGT 1 cut(s) 305
Sse9I AATT 2 cut(s) 52, 286
SsiI CCGC 5 cut(s) 9, 650, 1085, 1136, 1143
SspMI CTAG 2 cut(s) 306, 446
StyD4I CCNGG 7 cut(s) 342, 550, 551, 621, 909, 1012, 1047
StyI CCWWGG 2 cut(s) 380, 1109
TaaI ACNGT 6 cut(s) 248, 335, 587, 665, 741, 1092
TaiI ACGT 2 cut(s) 278, 873
TaqI TCGA 2 cut(s) 231, 635
TasI AATT 2 cut(s) 52, 286
TfiI GAWTC 6 cut(s) 145, 396, 842, 926, 1007, 1159
Tru1I TTAA 1 cut(s) 456
Tru9I TTAA 1 cut(s) 456
TscAI CASTG 2 cut(s) 1102, 1131
TseI GCWGC 3 cut(s) 44, 122, 532
TspDTI ATGAA 1 cut(s) 895
TspMI CCCGGG 1 cut(s) 551
TspRI CASTG 2 cut(s) 1102, 1131
VpaK11BI GGWCC 2 cut(s) 77, 1130
XceI RCATGY 1 cut(s) 342
XmaI CCCGGG 1 cut(s) 551
XspI CTAG 2 cut(s) 306, 446
Zsp2I ATGCAT 1 cut(s) 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.