Rmu_sc0001299.1_g000020

Tesmin/TSO1-like CXC domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001299.1
OG0004241
Physical Location & Seq
Reverse (-)
114318 .. 120024
5707 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2784 bp
atggacgggtcaccggaaacgaatcgggttgccgccgccgccgcctcatcgccggcccaagcctcgtctccggctgtgcaagtaactattcggttcttaatatctaagtgtgctgtaaacgccgtcgttttggagtcgcctttctccaatttccttagcaatctcactcctatcaatacagtgaagagtgatagttattcacaaagattagtaggagccttcttcccaacgccaccagttgtctttacatctccccatattgatctggagcgcgaaaccagtttcttggaaaggaatgatatagttgaagcaggttataaagaatgtaatacaactatattccagaaccctagttttgagaaggaggtccagttgtgcagtgcatcagggtgtattgatgaatatttggctgaccctgcgaaggtagactgtacaggttctgctgatatacagagcagtatcaatgccggcaaagaatccaatacggaagttcatgaggttattgatggtagtacaaagagtgaaccccttccaacattgaatcaagctgaagtgggtctgcctttaccttcactgacacagattgaaaccacaatcattgagaaggttgataaaaatagtgtagatcttgtcacccgagcacaaagccgtggacagaatacagctaaggagtcgcctttctccaatttccttagcaatctcactcctatcaatacagtgaagagtgatagttattcacaaagattagtaggagccttcttcccaacgccaccagttgtctttacatctccccatattgatctggagcgcgaaaccagtttcttggaaaggaatgatatagttgaagcaggttataaagaatgtaatacaactatattccagaaccctagttttgagaaggaggtccagttgtgcagtgcatcagggtgtattgatgaatatttggctgaccctgcgaaggtagactgtacaggttctgctgatatacagagcagtatcaatgccggcaaagaatccaatacggaagttcatgaggttattgatggtagtacaaagagtgaaccccttccaacattgaatcaagctgaagtgggtctgcctttaccttcactgacacagattgaaaccacaatcattgagaaggttgataaaaatagtgtagatcttgtcacccgagcacaaagccgtggacagaatacagctaaggaggttagtcaacaccaccgtggtatacgtaggcatctccaatttgagactgcagtagcccagaagagttctaactttggtaaccatcgaagtctttgcagtctgacgcatgatactgacaatttaaggtcacaatccaaactaacaaacttgaagactctagcttcttctcatttagatagcaaagccttcagcagtccgcagggtgttagctgtgacacttcacaattgtcttcttgcctctgtgagtcagttagatccagccaaattggtggaaactcttccacctcagctcctattcgttctggtattggtttacatctgaatagcattactcgatctacatccatgagctcagatgtattgctaagcaggaaagcaactggttgtctgagtacaccggaccagatgttggagcatgacaacagaaacaacatagaacatgagtcaagtagtagctttatttcagttgtacttgggcaaaattattcttctgtgggcaatgatcaacaagaaagtcaaaccgctgcgcacactactttcactttttattctactgatggtgtgccataccttccatgtgattctatgcatcagatactggttgaccagcactcagttccttctggagtattttgtttggattcatgtgcatgtgaaaactgctacaacaagcctgagtttgaggacactgtttttgatgcacgtcagcaaattgaatcccgtaatccgctggcatttgctcccaaagttgtgaagcatgaaatcaactcttcgcctaacattatggaagaagcagactggacgaccccatcatcagctaggcacaagagaggttgcaattgcaagaagtcaaagtgcctgaaaaaatactgtgaatgcttccaggctaatgttggatgttctggcgcatgtagatgtgattgttgccaaaatccgtgtggcactaaggcagaccatgaatacaaccgagctgaaaagtgggaaacccaccctgctgagaagctggatactattaagggtgggaatgattgcatcaaggccttggatatggatccgtactctccatgggaaggactttctgttataagcaatctcacgccattgtcaaatccttgctcaagtacaagggtttcttcagcttcatcaagtatgagaaaccgcacaaaaatttcgcaagcccaattacaatcaagtaggcttcagccatcaggtactggtcacttgaaaaggggacggtcacctgtcatatttaccccacaagtatttgaaagcaaggggccctctcagctcagttctgatggtgccagttatgaaagaatggatgatgatatacctgagttactgaaggaaccttctatcccccccaaggttgtcaaaggttcaccaaaccagaaacgtgtgtcacctccacaatcccgagctgagaagttgcggtcaaggtctcctaaaggcttgagaagtggccgcaaattcattttgcaggctatgccttctttccctcctctctccccgtacagcgattctaaagctatcaatgatactaggaatgaccataaaggtacccagaacaattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04218 map04218
Pfam Domains
Protein Families
TCR

Protein Analysis

927

Amino Acids

100.95

Weight (kDa)

5.88

Isoelectric Point (pI)

55.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TCR PF03638 679 - 714 4.8e-10 Tesmin/TSO1-like CXC domain, cysteine-rich domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 318, 855, 2294
Acc16I TGCGCA 1 cut(s) 1737
Acc36I ACCTGC 2 cut(s) 302, 839
Acc65I GGTACC 1 cut(s) 2768
AccB1I GGYRCC 2 cut(s) 2510, 2768
AccB7I CCANNNNNTGG 1 cut(s) 1618
AccI GTMKAC 3 cut(s) 426, 963, 1231
AccII CGCG 2 cut(s) 273, 810
AclWI GGATC 3 cut(s) 1458, 2255, 2268
AcoI YGGCCR 1 cut(s) 2671
AcsI RAATTY 2 cut(s) 2376, 2678
AcuI CTGAAG 6 cut(s) 570, 1107, 1381, 2328, 2393, 2573
AfiI CCNNNNNNNGG 5 cut(s) 421, 958, 1618, 2279, 2575
AflIII ACRYGT 1 cut(s) 2605
AjiI CACGTC 1 cut(s) 1913
AjnI CCWGG 1 cut(s) 2091
AleI CACNNNNGTG 1 cut(s) 1224
AloI GAACNNNNNNTCC 2 cut(s) 2550, 2582
Alw21I GWGCWC 3 cut(s) 643, 1180, 1562
Alw26I GTCTC 3 cut(s) 72, 1247, 2655
AlwI GGATC 3 cut(s) 1458, 2255, 2268
AlwNI CAGNNNCTG 4 cut(s) 440, 977, 1807, 2423
Ama87I CYCGRG 3 cut(s) 636, 1173, 2625
AoxI GGCC 4 cut(s) 54, 2247, 2486, 2671
ApaI GGGCCC 1 cut(s) 2490
ApeKI GCWGC 1 cut(s) 1733
ApoI RAATTY 2 cut(s) 2376, 2678
ArsI GACNNNNNNTTYG 2 cut(s) 1886, 1918
Asp700I GAANNNNTTC 3 cut(s) 528, 1065, 1486
Asp718I GGTACC 1 cut(s) 2768
AspLEI GCGC 4 cut(s) 273, 810, 1738, 2117
AspS9I GGNCC 6 cut(s) 55, 367, 904, 1609, 2486, 2487
AsuHPI GGTGA 6 cut(s) 3, 625, 1162, 2439, 2583, 2604
AvaI CYCGRG 3 cut(s) 636, 1173, 2625
AvaII GGWCC 3 cut(s) 367, 904, 1609
BaeGI GKGCMC 1 cut(s) 2490
BamHI GGATCC 1 cut(s) 2260
BanI GGYRCC 2 cut(s) 2510, 2768
BanII GRGCYC 2 cut(s) 1562, 2490
BbsI GAAGAC 2 cut(s) 1367, 1431
Bbv12I GWGCWC 3 cut(s) 643, 1180, 1562
BbvCI CCTCAGC 1 cut(s) 1495
BbvI GCAGC 1 cut(s) 1720
BccI CCATC 7 cut(s) 500, 1037, 1299, 1760, 2026, 2422, 2501
BceAI ACGGC 3 cut(s) 107, 633, 1170
BcgI CGANNNNNNTGC 2 cut(s) 1284, 1318
BciT130I CCWGG 1 cut(s) 2093
BciVI GTATCC 1 cut(s) 2209
BclI TGATCA 1 cut(s) 1711
BcoDI GTCTC 3 cut(s) 72, 1247, 2655
BfaI CTAG 5 cut(s) 351, 888, 1367, 2028, 2751
BfmI CTRYAG 1 cut(s) 1257
BfuAI ACCTGC 2 cut(s) 302, 839
BfuI GTATCC 1 cut(s) 2209
BglII AGATCT 2 cut(s) 625, 1162
BisI GCNGC 6 cut(s) 33, 36, 39, 42, 1734, 2674
BlpI GCTNAGC 1 cut(s) 1574
BlsI GCNGC 6 cut(s) 34, 37, 40, 43, 1735, 2675
Bme1390I CCNGG 1 cut(s) 2093
Bme18I GGWCC 3 cut(s) 367, 904, 1609
BmeT110I CYCGRG 3 cut(s) 636, 1173, 2625
BmgBI CACGTC 1 cut(s) 1913
BmgT120I GGNCC 6 cut(s) 55, 367, 904, 1609, 2486, 2487
BmiI GGNNCC 8 cut(s) 217, 754, 2262, 2487, 2488, 2512, 2559, 2770
BmrFI CCNGG 1 cut(s) 2093
BmsI GCATC 6 cut(s) 392, 929, 1249, 1807, 1897, 2250
BpiI GAAGAC 2 cut(s) 1367, 1431
BpmI CTGGAG 3 cut(s) 287, 824, 1854
Bpu10I CCTNAGC 5 cut(s) 155, 666, 692, 1203, 1495
Bpu1102I GCTNAGC 1 cut(s) 1574
BpuEI CTTGAG 2 cut(s) 2311, 2683
BsaAI YACGTR 1 cut(s) 1235
BsaBI GATNNNNATC 2 cut(s) 1549, 2259
BsaI GGTCTC 1 cut(s) 2655
BsaJI CCNNGG 6 cut(s) 649, 1186, 1225, 2250, 2273, 2574
BsaWI WCCGGW 2 cut(s) 13, 1606
BsaXI ACNNNNNCTCC 6 cut(s) 356, 386, 893, 923, 1483, 1513
Bsc4I CCNNNNNNNGG 5 cut(s) 421, 958, 1618, 2279, 2575
Bse118I RCCGGY 3 cut(s) 52, 467, 1004
Bse3DI GCAATG 1 cut(s) 1714
Bse8I GATNNNNATC 2 cut(s) 1549, 2259
BseBI CCWGG 1 cut(s) 2093
BseDI CCNNGG 6 cut(s) 649, 1186, 1225, 2250, 2273, 2574
BseGI GGATG 3 cut(s) 1550, 2111, 2536
BseJI GATNNNNATC 2 cut(s) 1549, 2259
BseLI CCNNNNNNNGG 5 cut(s) 421, 958, 1618, 2279, 2575
BseMI GCAATG 1 cut(s) 1714
BseRI GAGGAG 1 cut(s) 2700
BseSI GKGCMC 1 cut(s) 2490
BseXI GCAGC 1 cut(s) 1720
BsgI GTGCAG 2 cut(s) 397, 934
Bsh1236I CGCG 2 cut(s) 273, 810
BshFI GGCC 4 cut(s) 56, 2249, 2488, 2673
BshNI GGYRCC 2 cut(s) 2510, 2768
BsiHKAI GWGCWC 3 cut(s) 643, 1180, 1562
BsiHKCI CYCGRG 3 cut(s) 636, 1173, 2625
BsiSI CCGG 6 cut(s) 14, 53, 71, 468, 1005, 1607
BslFI GGGAC 1 cut(s) 2454
BslI CCNNNNNNNGG 5 cut(s) 421, 958, 1618, 2279, 2575
BsmAI GTCTC 3 cut(s) 72, 1247, 2655
BsmBI CGTCTC 1 cut(s) 72
BsmFI GGGAC 1 cut(s) 2454
BsmI GAATGC 1 cut(s) 2090
BsnI GGCC 4 cut(s) 56, 2249, 2488, 2673
Bso31I GGTCTC 1 cut(s) 2655
BsoBI CYCGRG 3 cut(s) 636, 1173, 2625
Bsp120I GGGCCC 1 cut(s) 2486
Bsp1286I GDGCHC 4 cut(s) 643, 1180, 1562, 2490
Bsp1407I TGTACA 2 cut(s) 431, 968
Bsp143I GATC 8 cut(s) 262, 625, 799, 1162, 1463, 1544, 1711, 2260
Bsp1720I GCTNAGC 1 cut(s) 1574
Bsp19I CCATGG 1 cut(s) 2273
BspANI GGCC 4 cut(s) 56, 2249, 2488, 2673
BspFNI CGCG 2 cut(s) 273, 810
BspHI TCATGA 2 cut(s) 493, 1030
BspLI GGNNCC 8 cut(s) 217, 754, 2262, 2487, 2488, 2512, 2559, 2770
BspMAI CTGCAG 1 cut(s) 1261
BspMI ACCTGC 2 cut(s) 302, 839
BspPI GGATC 3 cut(s) 1458, 2255, 2268
BspT107I GGYRCC 2 cut(s) 2510, 2768
BspTNI GGTCTC 1 cut(s) 2655
BsrDI GCAATG 1 cut(s) 1714
BsrFI RCCGGY 3 cut(s) 52, 467, 1004
BsrGI TGTACA 2 cut(s) 431, 968
BssAI RCCGGY 3 cut(s) 52, 467, 1004
BssECI CCNNGG 6 cut(s) 649, 1186, 1225, 2250, 2273, 2574
BssMI GATC 8 cut(s) 262, 625, 799, 1162, 1463, 1544, 1711, 2260
BssNAI GTATAC 1 cut(s) 1232
BssT1I CCWWGG 3 cut(s) 2250, 2273, 2574
Bst1107I GTATAC 1 cut(s) 1232
Bst2UI CCWGG 1 cut(s) 2093
Bst4CI ACNGT 8 cut(s) 181, 431, 718, 968, 1226, 1900, 2081, 2445
Bst6I CTCTTC 5 cut(s) 179, 716, 1265, 1492, 1984
BstAPI GCANNNNNTGC 1 cut(s) 2695
BstAUI TGTACA 2 cut(s) 431, 968
BstBAI YACGTR 1 cut(s) 1235
BstC8I GCNNGC 6 cut(s) 54, 469, 1006, 1941, 2385, 2691
BstDSI CCRYGG 4 cut(s) 649, 1186, 1225, 2273
BstEII GGTNACC 3 cut(s) 9, 1286, 2445
BstF5I GGATG 3 cut(s) 1550, 2111, 2536
BstFNI CGCG 2 cut(s) 273, 810
BstHHI GCGC 4 cut(s) 273, 810, 1738, 2117
BstKTI GATC 8 cut(s) 265, 628, 802, 1165, 1466, 1547, 1714, 2263
BstMAI GTCTC 3 cut(s) 72, 1247, 2655
BstMBI GATC 8 cut(s) 262, 625, 799, 1162, 1463, 1544, 1711, 2260
BstMWI GCNNNNNNNGC 7 cut(s) 38, 41, 119, 416, 953, 2494, 2695
BstNI CCWGG 1 cut(s) 2093
BstNSI RCATGY 2 cut(s) 1863, 2121
BstPI GGTNACC 3 cut(s) 9, 1286, 2445
BstSCI CCNGG 1 cut(s) 2091
BstSFI CTRYAG 1 cut(s) 1257
BstSLI GKGCMC 1 cut(s) 2490
BstSNI TACGTA 1 cut(s) 1235
BstUI CGCG 2 cut(s) 273, 810
BstV1I GCAGC 1 cut(s) 1720
BstV2I GAAGAC 2 cut(s) 1367, 1431
BstX2I RGATCY 4 cut(s) 625, 1162, 1463, 2260
BstXI CCANNNNNNTGG 3 cut(s) 286, 823, 1478
BstYI RGATCY 4 cut(s) 625, 1162, 1463, 2260
BstZ17I GTATAC 1 cut(s) 1232
BsuI GTATCC 1 cut(s) 2209
BsuRI GGCC 4 cut(s) 56, 2249, 2488, 2673
BtgI CCRYGG 4 cut(s) 649, 1186, 1225, 2273
BtgZI GCGATG 1 cut(s) 33
BtrI CACGTC 1 cut(s) 1913
BtsCI GGATG 3 cut(s) 1550, 2111, 2536
BtsI GCAGTG 2 cut(s) 385, 922
BtsIMutI CAGTG 7 cut(s) 186, 385, 572, 723, 922, 1109, 1896
BveI ACCTGC 2 cut(s) 302, 839
Cac8I GCNNGC 6 cut(s) 54, 469, 1006, 1941, 2385, 2691
CaiI CAGNNNCTG 4 cut(s) 440, 977, 1807, 2423
CciI TCATGA 2 cut(s) 493, 1030
CfoI GCGC 4 cut(s) 273, 810, 1738, 2117
Cfr10I RCCGGY 3 cut(s) 52, 467, 1004
Cfr13I GGNCC 6 cut(s) 55, 367, 904, 1609, 2486, 2487
CseI GACGC 1 cut(s) 1321
CspCI CAANNNNNGTGG 4 cut(s) 580, 615, 1117, 1152
DpnI GATC 8 cut(s) 264, 627, 801, 1164, 1465, 1546, 1713, 2262
DpnII GATC 8 cut(s) 262, 625, 799, 1162, 1463, 1544, 1711, 2260
EaeI YGGCCR 1 cut(s) 2671
Eam1104I CTCTTC 5 cut(s) 179, 716, 1265, 1492, 1984
EarI CTCTTC 5 cut(s) 179, 716, 1265, 1492, 1984
Ecl136II GAGCTC 1 cut(s) 1560
Eco105I TACGTA 1 cut(s) 1235
Eco130I CCWWGG 3 cut(s) 2250, 2273, 2574
Eco147I AGGCCT 1 cut(s) 2249
Eco24I GRGCYC 2 cut(s) 1562, 2490
Eco31I GGTCTC 1 cut(s) 2655
Eco47I GGWCC 3 cut(s) 367, 904, 1609
Eco53kI GAGCTC 1 cut(s) 1560
Eco57I CTGAAG 6 cut(s) 570, 1107, 1381, 2328, 2393, 2573
Eco88I CYCGRG 3 cut(s) 636, 1173, 2625
Eco91I GGTNACC 3 cut(s) 9, 1286, 2445
EcoICRI GAGCTC 1 cut(s) 1560
EcoO109I RGGNCCY 2 cut(s) 2486, 2487
EcoO65I GGTNACC 3 cut(s) 9, 1286, 2445
EcoRII CCWGG 1 cut(s) 2091
EcoT14I CCWWGG 3 cut(s) 2250, 2273, 2574
EcoT22I ATGCAT 1 cut(s) 1800
EcoT38I GRGCYC 2 cut(s) 1562, 2490
ErhI CCWWGG 3 cut(s) 2250, 2273, 2574
Esp3I CGTCTC 1 cut(s) 72
FalI AAGNNNNNCTT 2 cut(s) 2326, 2358
FaqI GGGAC 1 cut(s) 2454
FbaI TGATCA 1 cut(s) 1711
FblI GTMKAC 3 cut(s) 426, 963, 1231
Fnu4HI GCNGC 6 cut(s) 33, 36, 39, 42, 1734, 2674
FokI GGATG 3 cut(s) 1537, 2118, 2543
FriOI GRGCYC 2 cut(s) 1562, 2490
Fsp4HI GCNGC 6 cut(s) 33, 36, 39, 42, 1734, 2674
FspBI CTAG 5 cut(s) 351, 888, 1367, 2028, 2751
FspI TGCGCA 1 cut(s) 1737
GlaI GCGC 4 cut(s) 272, 809, 1737, 2116
GluI GCNGC 6 cut(s) 33, 36, 39, 42, 1734, 2674
GsuI CTGGAG 3 cut(s) 287, 824, 1854
HaeIII GGCC 4 cut(s) 56, 2249, 2488, 2673
HapII CCGG 6 cut(s) 14, 53, 71, 468, 1005, 1607
HgaI GACGC 1 cut(s) 1321
HhaI GCGC 4 cut(s) 273, 810, 1738, 2117
Hin6I GCGC 4 cut(s) 271, 808, 1736, 2115
HinP1I GCGC 4 cut(s) 271, 808, 1736, 2115
HincII GTYRAC 2 cut(s) 1217, 1813
HindII GTYRAC 2 cut(s) 1217, 1813
HpaII CCGG 6 cut(s) 14, 53, 71, 468, 1005, 1607
HphI GGTGA 6 cut(s) 3, 625, 1162, 2439, 2583, 2604
Hpy188I TCNGA 6 cut(s) 1311, 1530, 1564, 1599, 1803, 2506
Hpy188III TCNNGA 8 cut(s) 266, 343, 494, 803, 880, 1031, 1833, 2625
Hpy99I CGWCG 1 cut(s) 128
HpyCH4III ACNGT 8 cut(s) 181, 431, 718, 968, 1226, 1900, 2081, 2445
HpyCH4IV ACGT 3 cut(s) 1234, 1912, 2605
HpyF10VI GCNNNNNNNGC 7 cut(s) 38, 41, 119, 416, 953, 2494, 2695
HpySE526I ACGT 3 cut(s) 1234, 1912, 2605
HspAI GCGC 4 cut(s) 271, 808, 1736, 2115
KpnI GGTACC 1 cut(s) 2772
KroI GCCGGC 3 cut(s) 52, 467, 1004
KroNI GCCGGC 3 cut(s) 54, 469, 1006
Ksp22I TGATCA 1 cut(s) 1711
Kzo9I GATC 8 cut(s) 262, 625, 799, 1162, 1463, 1544, 1711, 2260
LmnI GCTCC 7 cut(s) 215, 268, 752, 805, 1504, 1621, 1954
Lsp1109I GCAGC 1 cut(s) 1720
LweI GCATC 6 cut(s) 392, 929, 1249, 1807, 1897, 2250
MaeI CTAG 5 cut(s) 351, 888, 1367, 2028, 2751
MaeII ACGT 3 cut(s) 1234, 1912, 2605
MalI GATC 8 cut(s) 264, 627, 801, 1164, 1465, 1546, 1713, 2262
MboI GATC 8 cut(s) 262, 625, 799, 1162, 1463, 1544, 1711, 2260
MfeI CAATTG 2 cut(s) 1433, 2047
MflI RGATCY 4 cut(s) 625, 1162, 1463, 2260
MhlI GDGCHC 4 cut(s) 643, 1180, 1562, 2490
MlyI GAGTC 5 cut(s) 143, 680, 1357, 1463, 1661
MmeI TCCRAC 4 cut(s) 557, 1094, 1599, 2083
Mph1103I ATGCAT 1 cut(s) 1800
MroNI GCCGGC 3 cut(s) 52, 467, 1004
MroXI GAANNNNTTC 3 cut(s) 528, 1065, 1486
MseI TTAA 3 cut(s) 98, 1331, 2223
MslI CAYNNNNRTG 5 cut(s) 388, 925, 1224, 1858, 2122
MspA1I CMGCKG 2 cut(s) 1733, 1939
MspI CCGG 6 cut(s) 14, 53, 71, 468, 1005, 1607
MspR9I CCNGG 1 cut(s) 2093
MunI CAATTG 2 cut(s) 1433, 2047
Mva1269I GAATGC 1 cut(s) 2090
MvaI CCWGG 1 cut(s) 2093
MvnI CGCG 2 cut(s) 273, 810
MwoI GCNNNNNNNGC 7 cut(s) 38, 41, 119, 416, 953, 2494, 2695
NaeI GCCGGC 3 cut(s) 54, 469, 1006
NcoI CCATGG 1 cut(s) 2273
NdeII GATC 8 cut(s) 262, 625, 799, 1162, 1463, 1544, 1711, 2260
NgoMIV GCCGGC 3 cut(s) 52, 467, 1004
NlaIV GGNNCC 8 cut(s) 217, 754, 2262, 2487, 2488, 2512, 2559, 2770
NmuCI GTSAC 8 cut(s) 9, 631, 1168, 1335, 1421, 2426, 2445, 2610
NsbI TGCGCA 1 cut(s) 1737
NsiI ATGCAT 1 cut(s) 1800
NspI RCATGY 2 cut(s) 1863, 2121
OliI CACNNNNGTG 1 cut(s) 1224
PagI TCATGA 2 cut(s) 493, 1030
PceI AGGCCT 1 cut(s) 2249
PctI GAATGC 1 cut(s) 2090
PdiI GCCGGC 3 cut(s) 54, 469, 1006
PdmI GAANNNNTTC 3 cut(s) 528, 1065, 1486
PfeI GAWTC 9 cut(s) 22, 476, 541, 1013, 1078, 1790, 1850, 1925, 2729
PflMI CCANNNNNTGG 1 cut(s) 1618
PkrI GCNGC 6 cut(s) 34, 37, 40, 43, 1735, 2675
PleI GAGTC 5 cut(s) 142, 679, 1357, 1462, 1660
PpsI GAGTC 5 cut(s) 142, 679, 1357, 1462, 1660
Ppu21I YACGTR 1 cut(s) 1235
PsiI TTATAA 3 cut(s) 318, 855, 2294
Psp124BI GAGCTC 1 cut(s) 1562
Psp6I CCWGG 1 cut(s) 2091
PspEI GGTNACC 3 cut(s) 9, 1286, 2445
PspGI CCWGG 1 cut(s) 2091
PspN4I GGNNCC 8 cut(s) 217, 754, 2262, 2487, 2488, 2512, 2559, 2770
PspOMI GGGCCC 1 cut(s) 2486
PspPI GGNCC 6 cut(s) 55, 367, 904, 1609, 2486, 2487
PstI CTGCAG 1 cut(s) 1261
PstNI CAGNNNCTG 4 cut(s) 440, 977, 1807, 2423
PsuI RGATCY 4 cut(s) 625, 1162, 1463, 2260
RseI CAYNNNNRTG 5 cut(s) 388, 925, 1224, 1858, 2122
SacI GAGCTC 1 cut(s) 1562
SaqAI TTAA 3 cut(s) 98, 1331, 2223
SatI GCNGC 6 cut(s) 33, 36, 39, 42, 1734, 2674
Sau3AI GATC 8 cut(s) 262, 625, 799, 1162, 1463, 1544, 1711, 2260
Sau96I GGNCC 6 cut(s) 55, 367, 904, 1609, 2486, 2487
SchI GAGTC 5 cut(s) 143, 680, 1357, 1463, 1661
ScrFI CCNGG 1 cut(s) 2093
SduI GDGCHC 4 cut(s) 643, 1180, 1562, 2490
SfaNI GCATC 6 cut(s) 392, 929, 1249, 1807, 1897, 2250
SfcI CTRYAG 1 cut(s) 1257
SinI GGWCC 3 cut(s) 367, 904, 1609
SmiMI CAYNNNNRTG 5 cut(s) 388, 925, 1224, 1858, 2122
SmlI CTYRAG 2 cut(s) 2326, 2662
SmoI CTYRAG 2 cut(s) 2326, 2662
SnaBI TACGTA 1 cut(s) 1235
SseBI AGGCCT 1 cut(s) 2249
SspI AATATT 2 cut(s) 404, 941
SspMI CTAG 5 cut(s) 351, 888, 1367, 2028, 2751
SstI GAGCTC 1 cut(s) 1562
StuI AGGCCT 1 cut(s) 2249
StyD4I CCNGG 1 cut(s) 2091
StyI CCWWGG 3 cut(s) 2250, 2273, 2574
TaaI ACNGT 8 cut(s) 181, 431, 718, 968, 1226, 1900, 2081, 2445
TaiI ACGT 3 cut(s) 1237, 1915, 2608
TaqI TCGA 2 cut(s) 1294, 1543
TatI WGTACW 7 cut(s) 431, 512, 968, 1049, 1601, 1678, 2330
TauI GCSGC 5 cut(s) 35, 38, 41, 44, 2676
TfiI GAWTC 9 cut(s) 22, 476, 541, 1013, 1078, 1790, 1850, 1925, 2729
Tru1I TTAA 3 cut(s) 98, 1331, 2223
Tru9I TTAA 3 cut(s) 98, 1331, 2223
TscAI CASTG 7 cut(s) 186, 385, 579, 723, 922, 1116, 1903
TseFI GTSAC 8 cut(s) 9, 631, 1168, 1335, 1421, 2426, 2445, 2610
TseI GCWGC 1 cut(s) 1733
Tsp45I GTSAC 8 cut(s) 9, 631, 1168, 1335, 1421, 2426, 2445, 2610
TspGWI ACGGA 4 cut(s) 500, 1037, 2133, 2253
TspRI CASTG 7 cut(s) 186, 385, 579, 723, 922, 1116, 1903
Van91I CCANNNNNTGG 1 cut(s) 1618
VpaK11BI GGWCC 3 cut(s) 367, 904, 1609
XapI RAATTY 2 cut(s) 2376, 2678
XceI RCATGY 2 cut(s) 1863, 2121
XcmI CCANNNNNNNNNTGG 2 cut(s) 2099, 2144
XmiI GTMKAC 3 cut(s) 426, 963, 1231
XmnI GAANNNNTTC 3 cut(s) 528, 1065, 1486
XspI CTAG 5 cut(s) 351, 888, 1367, 2028, 2751
Zsp2I ATGCAT 1 cut(s) 1800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.